المؤلفون: Nadia Baalbaki, Jelle M. Blankestijn, Mahmoud I. Abdel-Aziz, Jan de Backer, Somayeh Bazdar, Inés Beekers, Rosanne J. H. C. G. Beijers, Joop P. van den Bergh, Lizan D. Bloemsma, Harm Jan Bogaard, Job J. M. H. van Bragt, Vera van den Brink, Jean Paul Charbonnier, Merel E. B. Cornelissen, Yennece Dagelet, Elin Haf Davies, Anne M. van der Does, George S. Downward, Cornelis M. van Drunen, Debbie Gach, J. J. Miranda Geelhoed, Jorrit Glastra, Kornel Golebski, Irene H. Heijink, Judith C. S. Holtjer, Sebastiaan Holverda, Laura Houweling, John J. L. Jacobs, Renée Jonker, Renate Kos, Ramon C. J. Langen, Ivo van der Lee, Asabi Leliveld, Firdaus A. A. Mohamed Hoesein, Anne H. Neerincx, Lieke Noij, Johan Olsson, Marianne van de Pol, Simon D. Pouwels, Emiel Rolink, Michael Rutgers, Havva Șahin, Daphne Schaminee, Annemie M. W. J. Schols, Lisanne Schuurman, Gitte Slingers, Olie Smeenk, Brigitte Sondermeijer, Paul J. Skipp, Marisca Tamarit, Inge Verkouter, Roel Vermeulen, Rianne de Vries, Els J. M. Weersink, Marco van de Werken, Yolanda de Wit-van Wijck, Stewart Young, Esther J. Nossent, Anke H. Maitland-van der Zee

المصدر: Journal of Personalized Medicine, Vol 13, Iss 7, p 1060 (2023)

مصطلحات موضوعية: precision medicine, long COVID, infectious disease, prospective observational cohort study, Medicine

الوصف: Introduction: The coronavirus disease 2019 (COVID-19) pandemic has led to the death of almost 7 million people, however, with a cumulative incidence of 0.76 billion, most people survive COVID-19. Several studies indicate that the acute phase of COVID-19 may be followed by persistent symptoms including fatigue, dyspnea, headache, musculoskeletal symptoms, and pulmonary functional-and radiological abnormalities. However, the impact of COVID-19 on long-term health outcomes remains to be elucidated. Aims: The Precision Medicine for more Oxygen (P4O2) consortium COVID-19 extension aims to identify long COVID patients that are at risk for developing chronic lung disease and furthermore, to identify treatable traits and innovative personalized therapeutic strategies for prevention and treatment. This study aims to describe the study design and first results of the P4O2 COVID-19 cohort. Methods: The P4O2 COVID-19 study is a prospective multicenter cohort study that includes nested personalized counseling intervention trial. Patients, aged 40–65 years, were recruited from outpatient post-COVID clinics from five hospitals in The Netherlands. During study visits at 3–6 and 12–18 months post-COVID-19, data from medical records, pulmonary function tests, chest computed tomography scans and biological samples were collected and questionnaires were administered. Furthermore, exposome data was collected at the patient’s home and state-of-the-art imaging techniques as well as multi-omics analyses will be performed on collected data. Results: 95 long COVID patients were enrolled between May 2021 and September 2022. The current study showed persistence of clinical symptoms and signs of pulmonary function test/radiological abnormalities in post-COVID patients at 3–6 months post-COVID. The most commonly reported symptoms included respiratory symptoms (78.9%), neurological symptoms (68.4%) and fatigue (67.4%). Female sex and infection with the Delta, compared with the Beta, SARS-CoV-2 variant were significantly associated with more persisting symptom categories. Conclusions: The P4O2 COVID-19 study contributes to our understanding of the long-term health impacts of COVID-19. Furthermore, P4O2 COVID-19 can lead to the identification of different phenotypes of long COVID patients, for example those that are at risk for developing chronic lung disease. Understanding the mechanisms behind the different phenotypes and identifying these patients at an early stage can help to develop and optimize prevention and treatment strategies.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2075-4426/13/7/1060Test; https://doaj.org/toc/2075-4426Test

الوصول الحر: https://doaj.org/article/bc9d0c88602545a6b36cff2a76d5244aTest

المؤلفون: Ben Henderson, Gitte Slingers, Michele Pedrotti, Giovanni Pugliese, Michaela Malaskova, Luke Bryant, Tommaso Lomonaco, Silvia Ghimenti, Sergi Moreno, Rebecca Cordell, Frans JM Harren, Jochen Schubert, Chris A Mayhew, Michael Wilde, Fabio Di Francesco, Gudrun Koppen, Jonathan D Beauchamp, Simona M Cristescu

مصطلحات موضوعية: Uncategorized, Science & Technology, Life Sciences & Biomedicine, Biochemical Research Methods, Respiratory System, Biochemistry & Molecular Biology, standardization, breathomics, peppermint, benchmark, PTR-MS, SIFT-MS, pharmacokinetics, SUITABILITY, METABOLISM, BAGS

الوصف: A major challenge for breath research is the lack of standardization in sampling and analysis. To address this, a test that utilizes a standardized intervention and a defined study protocol has been proposed to explore disparities in breath research across different analytical platforms and to provide benchmark values for comparison. Specifically, the Peppermint Experiment involves the targeted analysis in exhaled breath of volatile constituents of peppermint oil after ingestion of the encapsulated oil. Data from the Peppermint Experiment performed by proton transfer reaction mass spectrometry (PTR-MS) and selected ion flow tube mass spectrometry (SIFT-MS) are presented and discussed herein, including the product ions associated with the key peppermint volatiles, namely limonene, α- and β-pinene, 1,8-cineole, menthol, menthone and menthofuran. The breath washout profiles of these compounds from 65 individuals were collected, comprising datasets from five PTR-MS and two SIFT-MS instruments. The washout profiles of these volatiles were evaluated by comparing the log-fold change over time of the product ion intensities associated with each volatile. Benchmark values were calculated from the lower 95% confidence interval of the linear time-to-washout regression analysis for all datasets combined. Benchmark washout values from PTR-MS analysis were 353 min for the sum of monoterpenes and 1,8-cineole (identical product ions), 173 min for menthol, 330 min for menthofuran, and 218 min for menthone; from SIFT-MS analysis values were 228 min for the sum of monoterpenes, 281 min for the sum of monoterpenes and 1,8-cineole, and 370 min for menthone plus 1,8-cineole. Large inter- and intra-dataset variations were observed, whereby the latter suggests that biological variability plays a key role in how the compounds are absorbed, metabolized and excreted from the body via breath. This variability seems large compared to the influence of sampling and analytical procedures, but further investigations are recommended to clarify ...

العلاقة: 2381/16817980.v1; https://figshare.com/articles/journal_contribution/The_peppermint_breath_test_benchmark_for_PTR-MS_and_SIFT-MS/16817980Test

الإتاحة: https://figshare.com/articles/journal_contribution/The_peppermint_breath_test_benchmark_for_PTR-MS_and_SIFT-MS/16817980Test

المؤلفون: M Benthe Muntinghe-Wagenaar, Hanneke Kievit, Lucie B. Hijmering-Kappelle, Birgitta I. Hiddinga, J Fred Ubbels, Robin Wijsman, Mechteld F. Brasz, Gitte Slingers, Rianne de Vries, Milou M. Schuurbiers, Harry J. Groen, Huib A. Kerstjens, Anthonie J. van der Wekken, Michel M. van den Heuvel, T Jeroen Hiltermann

المصدر: Cancer Research. 82:666-666

مصطلحات موضوعية: Cancer Research, Oncology

الوصف: It is increasingly understood that cancers can be recognized by the immune system and that inflammation relates to response. Combining stereotactic radiotherapy (SBRT) to increase release of cancer cell antigens with an anti-CTLA-4 and PD-L1 inhibitor may lead to increased response rates. Due to tumor heterogeneity it may be important to irradiate the primary tumor (addressing trunk mutations), instead of its metastasis (branch mutations). No solid predictive biomarker for response and/or immunotherapy related adverse events (TRAEs) is available. Electronic nose (eNose) technology measures the complete mixture of volatile organic compounds in exhaled breath and can detect lung cancer based on pattern recognition of the breath profile. We hypothesize that eNose is able to predict TRAEs and response in patients with NSCLC. Methods: In 3 sequential cohorts, immunotherapy regimes combined with SBRT were studied in stage IIIB/IV NSCLC patients progressing on chemotherapy. All patients were irradiated on the primary tumor (1x20 Gy on 9cc) 1 week after the 1st dose of immunotherapy. The 1st cohort (n=3) received durvalumab. The 2nd and 3rd cohort (both n=6) received a combination of durvalumab and tremelimumab followed by durvalumab monotherapy. Duplicate eNose measurements were performed by using the SpiroNose that contains 7 metal oxide semiconductor sensors. TRAEs were categorized using NCI CTCAE version 4.3. Descriptive statistics were used to summarize baseline characteristics and TRAEs. The relationship between breath profiles and response and TRAEs was analyzed with advanced signal processing, ambient correction and Mann-Whitney U test. Linear discriminant and receiver operating characteristics analysis followed. Pearson correlation and regression assessed duration of response. Findings: Fifteen patients were included as described above. Baseline characteristics of the groups were comparable. Median progression free survival was 2 months, overall survival 10 months (immature). No statistical difference was found in (duration of) response. There was 1 low grade TRAE (CTC 1-2) in cohort 1 and 10 in cohort 2/3. High grade TRAEs (CTC 3) were only present in cohort 2/3 (n=3) and 1 patient discontinued treatment. There was one dose limiting toxicity. At baseline, 12/15 patients performed eNose measurements; 7 with TRAEs and 5 without. The TRAE group had a significant higher sensor 7 signal compared to those without TRAE (p=.042). The cross-validated accuracy for detecting TRAE was 67%. The ROC-AUC was .857 [.638-1]. Interpretation: eNose breath profiles at baseline may predict which patients will develop TRAEs. The small sample size increases the risk of overfitting, therefore another cohort of 34 immuno-monotherapy patients is being analyzed (15 with and 19 without TRAEs, pending). We demonstrated no new safety data. Disclosure: This study was sponsored by a research grant from AstraZeneca. Citation Format: M Benthe Muntinghe-Wagenaar, Hanneke Kievit, Lucie B. Hijmering-Kappelle, Birgitta I. Hiddinga, J Fred Ubbels, Robin Wijsman, Mechteld F. Brasz, Gitte Slingers, Rianne de Vries, Milou M. Schuurbiers, Harry J. Groen, Huib A. Kerstjens, Anthonie J. van der Wekken, Michel M. van den Heuvel, T Jeroen Hiltermann. A phase 1 study to detect adverse events after SBRT and immunotherapy by electronic nose in advanced NSCLC [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 666.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::dca855ccfcca915ca09f5d137ec2bc65Test
https://doi.org/10.1158/1538-7445.am2022-666Test

المؤلفون: Gudrun Koppen, Maarten Spruyt, Marc Raes, Jill Lindekens, Eddy Goelen, Gitte Slingers, Martin Vanden Eede

المصدر: Rapid communications in mass spectrometry

مصطلحات موضوعية: Adult, Male, Analyte, Sorbent, Sample (material), Thermal desorption, 01 natural sciences, Mass Spectrometry, Analytical Chemistry, Young Adult, Desorption, Humans, Biology, Spectroscopy, Volatile Organic Compounds, Chromatography, Chemistry, 010401 analytical chemistry, Organic Chemistry, Direct sampling, Exhalation, Middle Aged, 0104 chemical sciences, Breath Tests, Female, Selected-ion flow-tube mass spectrometry

الوصف: Rationale Selected ion flow tube mass spectrometry (SIFT-MS) is versatile, rapidly provides result output and determines a wide range of volatiles, making it suitable for biomedical applications. When direct sampling into the SIFT-MS instrument is impractical, combining thermal desorption (TD) and SIFT-MS might offer a solution as it allows sample storage on sorbent tubes for later analysis. This work compares off-line TD SIFT-MS and real-time SIFT-MS for the quantification of selected breath volatiles. Methods Ten healthy non-smoking individuals provided 60 breath samples per method. For off-line analysis, breath was collected onto sorbent tubes via a breath sampler provided with filtered inspiratory air. After TD, samples were re-collected in Tedlar bags which were then connected to the SIFT-MS instrument. For real-time analysis, breath was sampled directly into the instrument. In both cases the analytical method included a total of 155 product ions, and 14 selected volatiles were quantified. The agreement between the methods was assessed using Pearson correlation coefficients and Bland-Altman plots. Results Overall, correlations between real-time and off-line analysis were moderate to very strong (r = 0.43-0.92) depending on the volatile of interest, except for 2,3-butanedione and styrene. The difference between real-time and off-line measured breath concentrations (average bias) ranged between -14.57 and 20.48 ppbv. For acetone and isoprene, it was 251.53 and 31.9 ppbv, respectively. Conclusions Real-time SIFT-MS and off-line TD SIFT-MS for quantification of selected breath volatiles did not show optimal agreement. Analyzing a multitude of analytes in breath via direct exhalation into a SIFT-MS instrument for real-time analysis is challenging. On the other hand, off-line analysis using a breath collection device also has its issues such as possible sample losses due to selective absorption depending on the sorbent used or during desorption and transfer to the instrument. Despite these drawbacks, both methods were moderately well correlated.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4845c2da98b0fe2b99087322f884befTest
https://doi.org/10.1002/rcm.8994Test

المؤلفون: Simona M. Cristescu, Silvia Ghimenti, Frans J. M. Harren, Chris A. Mayhew, Jonathan Beauchamp, Gudrun Koppen, Gitte Slingers, Sergi Moreno, Luke Bryant, Giovanni Pugliese, Fabio Di Francesco, Michele Pedrotti, Ben Henderson, Michael Wilde, Tommaso Lomonaco, Jochen K. Schubert, Rebecca Cordell, Michaela Malásková

المساهمون: Pedrotti, Michele/0000-0002-9383-9504, Pugliese, Giovanni/0000-0002-2869-3588, Lomonaco, Tommaso/0000-0002-1822-7399

المصدر: Journal of Breath Research, 15, 4, pp. 1-14
Journal of Breath Research, 15(4)
Journal of Breath Research 15 (2021) 4
Journal of Breath Research, 15, 1-14

مصطلحات موضوعية: Pulmonary and Respiratory Medicine, Menthofuran, Settore CHIM/01 - CHIMICA ANALITICA, SIFT-MS, Mass Spectrometry, breathomics, chemistry.chemical_compound, benchmark, medicine, Humans, Proton-transfer-reaction mass spectrometry, VLAG, peppermint, standardization, Breath test, Limonene, Chromatography, medicine.diagnostic_test, Washout, Mentha piperita, Menthone, PTR-MS, Benchmarking, Food Quality and Design, chemistry, Breath Tests, Selected-ion flow-tube mass spectrometry, Post Harvest Technology, Molecular and Laser Physics, Protons, Menthol, pharmacokinetics

الوصف: A major challenge for breath research is the lack of standardization in sampling and analysis. To address this, a test that utilizes a standardized intervention and a defined study protocol has been proposed to explore disparities in breath research across different analytical platforms and to provide benchmark values for comparison. Specifically, the Peppermint Experiment involves the targeted analysis in exhaled breath of volatile constituents of peppermint oil after ingestion of the encapsulated oil. Data from the Peppermint Experiment performed by proton transfer reaction mass spectrometry (PTR-MS) and selected ion flow tube mass spectrometry (SIFT-MS) are presented and discussed herein, including the product ions associated with the key peppermint volatiles, namely limonene, alpha- and beta-pinene, 1,8-cineole, menthol, menthone and menthofuran. The breath washout profiles of these compounds from 65 individuals were collected, comprising datasets from five PTR-MS and two SIFT-MS instruments. The washout profiles of these volatiles were evaluated by comparing the log-fold change over time of the product ion intensities associated with each volatile. Benchmark values were calculated from the lower 95% confidence interval of the linear time-to-washout regression analysis for all datasets combined. Benchmark washout values from PTR-MS analysis were 353 min for the sum of monoterpenes and 1,8-cineole (identical product ions), 173 min for menthol, 330 min for menthofuran, and 218 min for menthone; from SIFT-MS analysis values were 228 min for the sum of monoterpenes, 281 min for the sum of monoterpenes and 1,8-cineole, and 370 min for menthone plus 1,8-cineole. Large inter- and intra-dataset variations were observed, whereby the latter suggests that biological variability plays a key role in how the compounds are absorbed, metabolized and excreted from the body via breath. This variability seems large compared to the influence of sampling and analytical procedures, but further investigations are recommended to clarify the effects of these factors. Marie Skodowska-Curie Actions Innovative Training Network: Ion-Molecule Processes for Analytical Chemistry Technologies (IMPACT) through the European Commission's HORIZON 2020 Programme [674911]; UK's National Physical Laboratory

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d4127bc39b19fe9d51bdc5633ed3dd1Test
https://repository.ubn.ru.nl/handle/2066/237394Test

المؤلفون: Joline L.M. Millen, Gitte Slingers, Marc Raes, Ibbo Willems, Gudrun Koppen, Sabine A. S. Langie

المساهمون: Millen, Joline/0000-0002-1670-6820, RS: NUTRIM - R3 - Respiratory & Age-related Health, Farmacologie en Toxicologie

المصدر: Clinical and Experimental Immunology
Clinical and Experimental Immunology, 203(2), 183-193. Wiley

مصطلحات موضوعية: Male, 0301 basic medicine, Allergy, Respiratory Tract Diseases, medicine.disease_cause, Immunoglobulin E, immunoglobulin E, 0302 clinical medicine, Allergen, respiratory allergies, Immunology and Allergy, Multiplex, Immunoassay, Air Pollutants, biology, medicine.diagnostic_test, SKIN-PRICK, Middle Aged, Female, Original Article, Antibody, Adult, multiplex immunoassay, Immunology, high throughput, Sensitivity and Specificity, Young Adult, 03 medical and health sciences, Hypersensitivity, medicine, Humans, allergens, House dust mite, Reproducibility, business.industry, Reproducibility of Results, RHINITIS, immunocap, Original Articles, asthma, biology.organism_classification, medicine.disease, High-Throughput Screening Assays, 030104 developmental biology, biology.protein, business, 030215 immunology

الوصف: The performance of the MSD immunoassay, as an allergy blood test, was compared to the skin prick test (SPT; golden standard in clinical practices) and ImmunoCAP™ assay (most commonly used blood test). The MSD immunoassay, detecting sIgE against respiratory allergens, showed high sensitivity and specificity (compared to the ImmunoCAP™ assay or SPT), and low inter‐ and intra‐assay variation with good reproducibility.
Summary Allergic sensitization is commonly assessed in patients by performing the skin prick test (SPT) or determining specific immunoglobulin (IgE) levels in blood samples with the ImmunoCAP™ assay, which measures each allergen and sample separately. This paper explores the possibility to investigate respiratory allergies with a high throughput method, the Meso Scale Discovery (MSD) multiplex immunoassay, measuring IgE levels in low volumes of blood. The MSD multiplex immunoassay, developed and optimized with standards and allergens from Radim Diagnostics, was validated against the SPT and the ImmunoCAP assay. For 18 adults (15 respiratory allergy patients and three controls), blood collection and the SPT were performed within the same hour. Pearson correlations and Bland–Altman analysis showed high comparability of the MSD multiplex immunoassay with the SPT and the ImmunoCAP assay, except for house dust mite. The sensitivity of the MSD multiplexed assay was ≥78% for most allergens compared to the SPT and ImmunoCAP assay. Additionally, the specificity of the MSD multiplex immunoassay was ≥ 87% – the majority showing 100% specificity. Only the rye allergen had a low specificity when compared to the SPT, probably due to cross‐reactivity. The reproducibility of the MSD multiplex immunoassay, assessed as intra‐ and interassay reproducibility and biological variability between different sampling moments, showed significantly high correlations (r = 0·943–1) for all tested subjects (apart from subject 13; r = 0·65–0·99). The MSD multiplex immunoassay is a reliable method to detect specific IgE levels against respiratory allergens in a multiplexed and high‐throughput manner, using blood samples as small as from a finger prick.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ae5f68ebc3cebd9306e92e2718d733dTest
https://doi.org/10.22541/au.159183067.72852643Test

المؤلفون: Simona M. Cristescu, Andria Hadjithekli, Dorota Ruszkiewicz, C. L. Paul Thomas, Dahlia Salman, Olaf Holz, Gitte Slingers, Tommaso Lomonaco, Fabio Di Francesco, Flavio A. Franchina, Stanislas Grassin-Delyle, Jonathan Beauchamp, Max Wilkinson, Jens Langejürgen, Renato Zenobi, Stephen J. Fowler, Michael Wilde, Michaela Malásková, Chris A. Mayhew, Jean-François Focant, Michele Pedrotti, Delphine Zanella, Ben Henderson, Giovanni Pugliese, Gudrun Koppen, Pablo Martinez-Lozano Sinues, Pierre-Hugues Stefanuto, Sergi Moreno

المساهمون: Radboud University Medical Centre [Nijmegen, The Netherlands], Loughborough University, Manchester Academic Health Science Centre (MAHSC), University of Manchester [Manchester], Fraunhofer-Institut für Verfahrenstechnik und Verpackung IVV (Fraunhofer IVV), University of Pisa - Università di Pisa, VITO, Partenaires INRAE, German Center for Lung Research, National Physical Laboratory [Teddington] (NPL), Fondazione Edmund Mach - Edmund Mach Foundation [Italie] (FEM), University Children's Hospital [Basel, Switzerland]], Hasselt University (UHasselt), University of Leicester, Molecular Systems Research Unit, University of Liège, Université de Liège, Department of Chemistry and Applied Biosciences [ETH Zürich] (D-CHAB), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Infection et inflammation (2I), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Foch [Suresnes], University of Innsbruck, University of Rostock, University of Birmingham [Birmingham], European Project: 674911,H2020,H2020-MSCA-ITN-2015,IMPACT(2016), Beauchamp, Jonathan/0000-0003-1405-7625, Salman, Dr, Dahlia/0000-0002-5354-2407, Di Francesco, Fabio/0000-0002-9285-1595, Franchina, Flavio Antonio/0000-0001-7236-4266, Slingers, Gitte/0000-0002-7021-5125, Zanella, Delphine/0000-0002-0131-2837, Wilkinson, Max/0000-0002-4924-612X, Radboud University Medical Center [Nijmegen], Leopold Franzens Universität Innsbruck - University of Innsbruck, Publica

المصدر: Journal of Breath Research
Journal of Breath Research, IOP Science, 2020, 14 (4), ⟨10.1088/1752-7163/aba130⟩
Journal of Breath Research 14 (2020) 4
Journal of Breath Research, 14, 4, pp. 1-10
Journal of Breath Research, 14, 1-10
Journal of Breath Research, 14(4)
Journal of Breath Research, 2020, 14 (4), ⟨10.1088/1752-7163/aba130⟩

مصطلحات موضوعية: Male, Pulmonary and Respiratory Medicine, Settore CHIM/01 - CHIMICA ANALITICA, Repeated sampling, Sample (material), [SDV]Life Sciences [q-bio], 01 natural sciences, NO, 03 medical and health sciences, breathomics, 0302 clinical medicine, benchmark, Statistics, Humans, Pharmacokinetics, Protocol (science), peppermint, standardization, Volatile Organic Compounds, repeated sampling8pharmacokinetics, 010401 analytical chemistry, Breath sampling, Sampling (statistics), Mentha piperita, Standardization, Breathomics, Peppermint, Repeated sampling, Pharmacokinetics, Benchmark, Replicate, Benchmarking, 3. Good health, 0104 chemical sciences, Breath Tests, 030228 respiratory system, Breath gas analysis, Environmental science, Female, Post Harvest Technology, Molecular and Laser Physics, Gas chromatography–mass spectrometry

الوصف: Sampling of volatile organic compounds (VOCs) has shown promise for detection of a range of diseases but results have proved hard to replicate due to a lack of standardization. In this work we introduce the 'Peppermint Initiative'. The initiative seeks to disseminate a standardized experiment that allows comparison of breath sampling and data analysis methods. Further, it seeks to share a set of benchmark values for the measurement of VOCs in breath. Pilot data are presented to illustrate the standardized approach to the interpretation of results obtained from the Peppermint experiment. This pilot study was conducted to determine the washout profile of peppermint compounds in breath, identify appropriate sampling time points, and formalise the data analysis. Five and ten participants were recruited to undertake a standardized intervention by ingesting a peppermint oil capsule that engenders a predictable and controlled change in the VOC profile in exhaled breath. After collecting a pre-ingestion breath sample, five further samples are taken at 2, 4, 6, 8, and 10 h after ingestion. Samples were analysed using ion mobility spectrometry coupled to multi-capillary column and thermal desorption gas chromatography mass spectrometry. A regression analysis of the washout data was used to determine sampling times for the final peppermint protocol, and the time for the compound measurement to return to baseline levels was selected as a benchmark value. A measure of the quality of the data generated from a given technique is proposed by comparing data fidelity. This study protocol has been used for all subsequent measurements by the Peppermint Consortium (16 partners from seven countries). So far 1200 breath samples from 200 participants using a range of sampling and analytical techniques have been collected. The data from the consortium will be disseminated in subsequent technical notes focussing on results from individual platforms. The Marie Sklodowska-Curie Actions Innovative Training Network: Ion-Molecule Processes for Analytical Chemistry Technologies (IMPACT) (www.impact-h2020itn.com), which has supported BH, MM and GP as Early Stage Researchers through the European Commission's HORIZON 2020 Programme under Grant Agreement Number 674911. The EMBER consortium for support in the dissemination of the Peppermint Initiative; researchers involved in the application of the protocol to specific techniques (to be published in due course) Abigail Joyce, Amisha Manek, Bellagambi Francesca Giuseppa, Bonsignori Alessio, Ghimenti Silvia and Biagini Denise, Liesl Carr, Luke Bryant and Rebecca Cordell. MW and SJF are supported by the NIHR Manchester Biomedical Research Centre. The group from Li`ege University is supported by the FNRS through the EOS and the FRIA initiatives. Ruszkiewicz, DM (, orresponding author), Loughborough Univ, Ctr Analyt Sci, Dept Chem, Loughborough, Leics, England ; Univ Birmingham, Sch Phys & Astron, Mol Phys Grp, Birmingham, W Midlands, England. D.M.Ruszkiewicz2@lboro.ac.uk

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cdaa433b1043893f97c16daf5960b1f6Test
https://hal.archives-ouvertes.fr/hal-02984272Test

المؤلفون: Gitte Slingers, Marc Raes, Joris J. T. H. Roelofs, Pouline M.P. van Oort, Stephen J. Fowler, Dennis C J J Bergmans, Ronny M. Schnabel, Royston Goodacre, Adrie Maas, Paul Brinkman, Lieuwe D. J. Bos, Gudrun Koppen, Marcus J. Schultz

المساهمون: Graduate School, ACS - Heart failure & arrhythmias, ARD - Amsterdam Reproduction and Development, Pathology, ACS - Diabetes & metabolism, ACS - Pulmonary hypertension & thrombosis, Intensive Care Medicine, AII - Infectious diseases, ACS - Microcirculation, MUMC+: MA Medische Staf IC (9), Surgery, RS: NUTRIM - R2 - Liver and digestive health, MUMC+: MA Arts Assistenten IC (9), Intensive Care

المصدر: American journal of physiology. Lung cellular and molecular physiology
van Oort, P M, Brinkman, P, Slingers, G, Koppen, G, Maas, A, Roelofs, J J, Schnabel, R, Bergmans, D C, Raes, M, Goodacre, R, Fowler, S J, Schultz, M J, Bos, L D & BreathDx Consortium 2019, ' Exhaled breath metabolomics reveals a pathogen-specific response in a rat pneumonia model for two human pathogenic bacteria: A proof-of-concept study ', American Journal of Physiology-Lung Cellular and Molecular Physiology, vol. 316, no. 5, pp. L751-L756 . https://doi.org/10.1152/ajplung.00449.2018Test
American journal of physiology. Lung cellular and molecular physiology, 316(5), L751-L756. American Physiological Society
American Journal of Physiology-Lung Cellular and Molecular Physiology, 316(5), L751-L756. American Physiological Society

مصطلحات موضوعية: Male, Pulmonary and Respiratory Medicine, Physiology, medicine.medical_treatment, VOLATILE ORGANIC-COMPOUNDS, medicine.disease_cause, 01 natural sciences, Gas Chromatography-Mass Spectrometry, Microbiology, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, Physiology (medical), Streptococcus pneumoniae, Animals, Humans, Metabolomics, Medicine, pneumonia, Pseudomonas Infections, Saline, Pathogen, business.industry, Pseudomonas aeruginosa, 010401 analytical chemistry, Respiratory infection, biomarkers, exhaled breath analysis, Pathogenic bacteria, Cell Biology, MASS-SPECTROMETRY, Pneumonia, Pneumococcal, medicine.disease, infection, Rats, 0104 chemical sciences, Disease Models, Animal, Pneumonia, Breath Tests, 030228 respiratory system, Breath gas analysis, Metabolome, business

الوصف: Volatile organic compounds in breath can reflect host and pathogen metabolism and might be used to diagnose pneumonia. We hypothesized that rats with Streptococcus pneumoniae ( SP) or Pseudomonas aeruginosa ( PA) pneumonia can be discriminated from uninfected controls by thermal desorption-gas chromatography-mass-spectrometry (TD-GC-MS) and selected ion flow tube-mass spectrometry (SIFT-MS) of exhaled breath. Male adult rats ( n = 50) received an intratracheal inoculation of 1) 200 µl saline, or 2) 1 × 107 colony-forming units of SP or 3) 1 × 107 CFU of PA. Twenty-four hours later the rats were anaesthetized, tracheotomized, and mechanically ventilated. Exhaled breath was analyzed via TD-GC-MS and SIFT-MS. Area under the receiver operating characteristic curves (AUROCCs) and correct classification rate (CCRs) were calculated after leave-one-out cross-validation of sparse partial least squares-discriminant analysis. Analysis of GC-MS data showed an AUROCC (95% confidence interval) of 0.85 (0.73–0.96) and CCR of 94.6% for infected versus noninfected animals, AUROCC of 0.98 (0.94–1) and CCR of 99.9% for SP versus PA, 0.92 (0.83–1.00), CCR of 98.1% for SP versus controls and 0.97 (0.92–1.00), and CCR of 99.9% for PA versus controls. For these comparisons the SIFT-MS data showed AUROCCs of 0.54, 0.89, 0.63, and 0.79, respectively. Exhaled breath analysis discriminated between respiratory infection and no infection but with even better accuracy between specific pathogens. Future clinical studies should not only focus on the presence of respiratory infection but also on the discrimination between specific pathogens.

وصف الملف: application/pdf; application/vnd.openxmlformats-officedocument.wordprocessingml.document

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aeabbb5b50c934caf5df80e3be258d83Test
http://livrepository.liverpool.ac.uk/3077711/1/ajplung.00449.2018.pdfTest

المؤلفون: Marcus J. Schultz, Ronny M. Schnabel, Adrie Maas, Paul Brinkman, Gudrun Koppen, Joris J. T. H. Roelofs, Dennis C J J Bergmans, Gitte Slingers, Pouline M.P. van Oort, Stephen J. Fowler, Roy Goodacre, Lieuwe D. J. Bos

المصدر: Respiratory infections.

مصطلحات موضوعية: Colony-forming unit, Lung, business.industry, Pseudomonas aeruginosa, Respiratory infection, medicine.disease, medicine.disease_cause, Microbiology, Pneumonia, medicine.anatomical_structure, Breath gas analysis, Streptococcus pneumoniae, medicine, business, Pathogen

الوصف: Volatile organic compounds (VOCs) in breath can reflect host and pathogen metabolism and might be used to diagnose pneumonia (Oort J Breath Res 2018). We hypothesized that rats with Streptococcus pneumoniae (SP) or Pseudomonas aeruginosa (PA) pneumonia can be discriminated from uninfected controls by gas-chromatography mass-spectrometry (GC-MS) of exhaled breath. Male adult rats (N = 50) received an intra-tracheal inoculation of 1) 200µL saline, 2) 1x107 colony forming units (cfu) of SP or 3) 1x107 cfu of PA. 24 hours later the rats were anaesthetized, tracheotomized and mechanically ventilated. Exhaled breath was analyzed via GC-MS. Area under the receiver operating characteristic curve (AUROCC) was calculated after leave-one-out cross-validation of sparse partial least squares discriminant analysis (sPLS-DA). The AUROCC was 0.85 (95%-CI: 0.73-0.96) for infected vs. non-infected animals. The AUROCC was 0.98 (95%-CI: 0.94-1) for SP vs. PA, 0.92 (95%-CI: 0.83-1) for SP vs. controls and 0.97 (95%-CI: 0.92-1) for PA vs. controls. Exhaled breath analysis discriminated with good accuracy between respiratory infection and no infection, but with much better accuracy between the specific pathogens. Clinical studies should focus more on the discrimination between specific pathogens, instead of the general presence of respiratory infection.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::0ce19ce75b58274a89a8d4bbb677ea4aTest
https://doi.org/10.1183/13993003.congress-2018.pa2659Test

المؤلفون: Marcus J. Schultz, Philippe G. Jorens, Marc Raes, Gudrun Koppen, Joris J. T. H. Roelofs, Adrie Maas, Gitte Slingers, Pouline M.P. van Oort, Lieuwe D. J. Bos

المصدر: Respiratory infections.

مصطلحات موضوعية: Colony-forming unit, Chromatography, Breath gas analysis, business.industry, Pseudomonas aeruginosa, Streptococcus pneumoniae, Rat model, Medicine, Selected-ion flow-tube mass spectrometry, Respiratory system, Gas chromatography–mass spectrometry, business, medicine.disease_cause

الوصف: Exhaled volatile organic compounds (VOCs) could potentially serve as non-invasive biomarkers for the diagnosis of respiratory infections. This study aims to discriminate rats infected with Pseudomonas aeruginosa (PA) or Streptococcus pneumoniae (SP) from non-infected rats. Exhaled breath was collected from 50 male rats 24h after intra-tracheal inoculation of 200µL saline, or 1x107 colony forming units of SP or PA. VOCs in exhaled breath trapped on sorbent tubes were analysed with Selected Ion Flow Tube Mass Spectrometry (SIFT-MS) after thermal desorption. The pattern of reaction ions with mass/charge ratio 15 to 200 was analysed using sparse partial least squares discriminant analysis. Area under the receiver operating characteristic curve (AUROCC) calculated after leave-one-out cross-validation of the multivariate discriminant analysis was 0.73 (95%CI: 0.57-0.89) for infected vs. non-infected animals. For SP vs. PA the AUROCC was 0.97 (95%CI: 0.93-1) while for SP vs. controls and PA vs. controls it was 0.67 (95%CI: 0.47-0.86) and 0.72 (95%CI: 0.53-0.91), respectively. Exhaled breath analysis discriminated with higher accuracy between animals infected with specific respiratory pathogens compared to infected vs. non-infected animals. Similar findings were observed for VOCs analysed with Gas Chromatography Mass Spectrometry (see submitted ERS abstract from P. M. van Oort et al.). Our results suggest that breath analysis in infected patients may be helpful in discriminating different pathogens. Furthermore, SIFT-MS has potential as a point-of-care tool to assess respiratory infections in clinical practice.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::d0a332b60b0b122c89a15d57325d7b83Test
https://doi.org/10.1183/13993003.congress-2018.pa2653Test