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المؤلفون: Gisela Paola Lazzarino
المصدر: FABICIB; Vol. 24 (2020): FABICIB
FABICIB; Vol 24 (2020): FABICIB
Biblioteca Virtual (UNL)
Universidad Nacional del Litoral
instacron:UNLمصطلحات موضوعية: Biology, Humanities
الوصف: Fil: Lazzarino, Gisela Paola. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas; Argentina.
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f7223dfc4740adef089136ae06a4c18Test
https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FABICIB/article/view/10755Test -
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المصدر: Journal of neuroimmunology. 342
مصطلحات موضوعية: Immunology, 03 medical and health sciences, 0302 clinical medicine, medicine, Immunology and Allergy, Receptor, Interleukin 6, Immune cell infiltration, Demyelination, Interleukin-6, Microglia, Multiple sclerosis, EAE, Endothelial cells, biology, business.industry, Cell adhesion molecule, Experimental autoimmune encephalomyelitis, Neurosciences, Interleukin, medicine.disease, medicine.anatomical_structure, Neurology, biology.protein, Neurology (clinical), business, Neurovetenskaper, 030217 neurology & neurosurgery, 030215 immunology
الوصف: Experimental autoimmune encephalomyelitis (EAE) is the most common model for studying the molecular mechanisms of multiple sclerosis (MS). Here, we examined the CNS-restricted effects of classical interleukin (IL)6 signaling on the development of EAE, using mice with cell-type specific deletion of the IL-6 receptor (IL-6R). We found that IL-6R deletion in CNS vascular endothelial cells, but not in microglia, ameliorated symptoms of EAE. The milder clinical symptoms in the gene-deleted mice were associated with less demyelination and immune cell infiltration/activation, and lower mRNA levels of the cytokines IL-17 and IL-1 beta, as well as the cell adhesion molecules VCAM-1, ICAM-1 and ICAM-2 than what was seen in WT mice. These findings demonstrate that classical IL-6 signaling via endothelial cells of the CNS contributes substantially to the development of MS-like pathology, which should be taken into consideration when conceptualizing future therapeutic approaches. Postprint version uploaded on author’s personal web page: https://liu.se/medarbetare/andbl47FundingTest Agencies|Swedish Research CouncilSwedish Research Council [2016-01301]; Swedish Brain Foundation [FO2019-0033]; Foundation for Parkinson Research at Linkoping University
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f08e48947607d14880348dc71a7ca65Test
https://pubmed.ncbi.nlm.nih.gov/32182451Test -
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المؤلفون: Maria Florencia Andreoli, María Florencia Rossetti, Rocio Schumacher, Jorge G. Ramos, Cora Stoker, Jorgelina Varayoud, María Paula Gastiazoro, Gisela Paola Lazzarino
مصطلحات موضوعية: Male, 0301 basic medicine, medicine.medical_specialty, CIENCIAS MÉDICAS Y DE LA SALUD, Dietary Sugars, Offspring, RAT OFFSPRING, Ciencias de la Salud, DOPAMINE-RELATED GENES, Biology, Nucleus Accumbens, Epigenesis, Genetic, 03 medical and health sciences, 0302 clinical medicine, Pregnancy, Dopamine, Internal medicine, medicine, Animals, Rats, Wistar, MATERNAL CAFETERIA DIET, Dopamine transporter, EPIGENETIC, Receptors, Dopamine D2, Dopaminergic Neurons, General Neuroscience, Dopaminergic, Maternal Nutritional Physiological Phenomena, Dietary Fats, Rats, Ventral tegmental area, Otras Ciencias de la Salud, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, Animals, Newborn, Dopamine receptor, DNA methylation, biology.protein, Female, Ghrelin, Energy Intake, 030217 neurology & neurosurgery, medicine.drug
الوصف: Dopamine is a neurotransmitter crucial for motor, motivational, and reward-related functions. Our aim was to determine the effect of a palatable maternal diet on the transcriptional regulation of dopaminergic-related genes during perinatal development of the offspring. For that, female offspring from dams fed with a control (CON) or a cafeteria (CAF) diet were sacrificed on embryonic day 21 (E21) and postnatal day 10 (PND10). Using micropunch techniques, ventral tegmental area (VTA) and nucleus accumbens (NAc) were isolated from brain´s offspring. Bioinformatic analysis of the promoter regions, mRNA quantification and methylation studies were done. The increase in tyroxine hidroxylase (TH), dopamine receptor (DRD) 1 and ghrelin receptor (GHSR) expression in VTA and NAc from E21 to PND10 was correlated with changes in DNA methylation of their promoter regions. Maternal diet did not affect the expressionpatternsin E21. At PND10, maternal CAF diet decreased the transcription of TH, GHSR, DRD2 and dopamine transporter (DAT) in VTA. Interestingly, the changes in TH, DRD2 and DAT expression were related to the methylation status of their promoters. In NAc, maternal CAF diet reduced DRD1, DRD2 and DAT expression in the offspring at PND10, although alternations in the methylation patterns were only detected in DAT promoter. These results show the importance of maternal nutrition and provide novel insights into the mechanisms through which maternal junk-food feeding can affect reward system during development and early postnatal life. Particularly important is the expression decline of DRD2 given its physiological implication in obesity and addiction. Fil: Rossetti, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Schumacher, Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Gastiazoro, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Lazzarino, Gisela Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Andreoli, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Stoker, Cora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Varayoud, Jorgelina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Ramos, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c8b83ffb00987271c5b0eeecb17bd9dTest
https://linkinghub.elsevier.com/retrieve/pii/S030645221930661XTest -
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المؤلفون: Maria Florencia Andreoli, Guillermina Canesini, Gisela Paola Lazzarino, Ana Paula García, Rocio Schumacher, Cora Stoker, Jorge G. Ramos, María Florencia Rossetti
المصدر: Molecular and Cellular Endocrinology. 522:111123
مصطلحات موضوعية: Male, 0301 basic medicine, endocrine system, medicine.medical_specialty, Pro-Opiomelanocortin, Time Factors, 030209 endocrinology & metabolism, Biology, Biochemistry, Cocaine and amphetamine regulated transcript, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Internal medicine, medicine, Animals, Homeostasis, Weaning, RNA, Messenger, Rats, Wistar, Promoter Regions, Genetic, Receptor, Molecular Biology, Epididymis, Leptin, Body Weight, Neuropeptides, digestive, oral, and skin physiology, Arcuate Nucleus of Hypothalamus, Gene Expression Regulation, Developmental, Feeding Behavior, DNA Methylation, Neuropeptide Y receptor, 030104 developmental biology, Adipose Tissue, Animals, Newborn, DNA methylation, Metabolome, Developmental plasticity, CpG Islands, Ghrelin, hormones, hormone substitutes, and hormone antagonists
الوصف: A small litter (SL) model was used to determine how neonatal overfeeding affects the homeostatic control of food intake in male rats at weaning and postnatal day (PND) 90. At PND4, litters were reduced to small (4 pups/dam) or normal (10 pups/dam) litters. At weaning, SL rats showed higher body weight and characteristic features of the metabolic syndrome. Gene expression of pro-opiomelanocortin (POMC), cocaine and amphetamine regulated transcript, neuropeptide Y (NPY) and leptin and ghrelin (GHSR) receptors were increased and POMC promoter was hypomethylated in arcuate nucleus, indicating that the early development of obesity may involve the GHSR/NPY system and changes in POMC methylation state. At PND90, body weight, metabolic parameters and gene expression were restored; however, POMC methylation state remained altered. This work provides insight into the effects of neonatal overfeeding, showing the importance of developmental plasticity in restoring early changes in central pathways involved in metabolic programming.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4698d6b1f2812523e98399360f46a4c1Test
https://doi.org/10.1016/j.mce.2020.111123Test -
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المؤلفون: Cora Stoker, Jorge G. Ramos, María V. Tschopp, María Florencia Rossetti, Gisela Paola Lazzarino, Maria Florencia Andreoli, Enrique H. Luque
المصدر: Molecular and Cellular Endocrinology. 450:113-125
مصطلحات موضوعية: 0301 basic medicine, Transcription, Genetic, medicine.medical_treatment, Receptors, Cytoplasmic and Nuclear, Biochemistry, Eating, Endocrinology, Kisspeptin, Receptor, DNA METHYLATION, digestive, oral, and skin physiology, Organ Size, Adipose Tissue, Hormone receptor, NEUROSTEROIDOGENIC ENZYMES, DNA methylation, Female, Steroids, CIENCIAS NATURALES Y EXACTAS, hormones, hormone substitutes, and hormone antagonists, medicine.drug, medicine.medical_specialty, NEUROPEPTIDES, Otras Ciencias Biológicas, Hypothalamus, Neuropeptide, Biology, Ciencias Biológicas, HORMONE RECEPTORS, 03 medical and health sciences, Internal medicine, Orexigenic, medicine, Animals, Computer Simulation, RNA, Messenger, Epigenetics, Rats, Wistar, Molecular Biology, Binding Sites, Body Weight, Neuropeptides, Arcuate Nucleus of Hypothalamus, DNA Methylation, Glucose Tolerance Test, Diet, Steroid hormone, 030104 developmental biology, Gene Expression Regulation, Ventromedial Hypothalamic Nucleus, CAFETERIA DIET, Energy Intake, Paraventricular Hypothalamic Nucleus
الوصف: We evaluated the effect of cafeteria diet (CAF) on the mRNA levels and DNA methylation state of feeding-related neuropeptides, and neurosteroidogenic enzymes in discrete hypothalamic nuclei. Besides, the expression of steroid hormone receptors was analyzed. Female rats fed with CAF from weaning increased their energy intake, body weight, and fat depots, but did not develop metabolic syndrome. The increase in energy intake was related to an orexigenic signal of paraventricular (PVN) and ventromedial (VMN) nuclei, given principally by upregulation of AgRP and NPY. This was mildly counteracted by the arcuate nucleus, with decreased AgRP expression and increased POMC and kisspeptin expression. CAF altered the transcription of neurosteroidogenic enzymes in PVN and VMN, and epigenetic mechanisms associated with differential promoter methylation were involved. The changes observed in the hypothalamic nuclei studied could add information about their differential role in food intake control and how their action is disrupted in obesity. Fil: Lazzarino, Gisela Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Andreoli, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Rossetti, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Stoker, Cora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Tschopp, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Ramos, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ddcf7bbe1291fcf5ccc072b5e037ca14Test
https://doi.org/10.1016/j.mce.2017.05.005Test -
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المؤلفون: Maria Florencia Andreoli, Jorge G. Ramos, Cora Stoker, Gisela Paola Lazzarino, Enrique H. Luque, María Florencia Rossetti
المصدر: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICETمصطلحات موضوعية: Male, 0301 basic medicine, Cholestenone 5 alpha-Reductase, Endocrinology, Diabetes and Metabolism, Gene Expression, Estrogen receptor, Eating, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, Neurotransmitter Agents, Nutrition and Dietetics, Steroidogenic acute regulatory protein, food and beverages, purl.org/becyt/ford/3.1 [https], Medicina Básica, Hypothalamus, purl.org/becyt/ford/3 [https], Signal Transduction, endocrine system, medicine.medical_specialty, 3-Hydroxysteroid Dehydrogenases, CIENCIAS MÉDICAS Y DE LA SALUD, Neuroactive steroid, Neurociencias, Alpha (ethology), Phytoestrogens, Biology, Diet, High-Fat, 03 medical and health sciences, Aromatase, Internal medicine, medicine, Animals, Obesity, Rats, Wistar, Steroidogenic Enzymes, Appetite Regulation, urogenital system, Cholesterol side-chain cleavage enzyme, Allopregnanolone, Estrogen Receptor alpha, Diet, 030104 developmental biology, chemistry, Energy Intake, 030217 neurology & neurosurgery, Erα
الوصف: Removing dietary phytoestrogens causes obesity and diabetes in adult male rats. Based on the facts that hypothalamic food intake control is disrupted in phytoestrogen-deprived animals and that several steroids affect food intake, we hypothesized that phytoestrogen withdrawal alters the expression of hypothalamic steroidogenic enzymes. Male Wistar rats fed with a high-phytoestrogen diet from conception to adulthood were subjected to phytoestrogen withdrawal by feeding them a low-phytoestrogen diet or a high-phytoestrogen, high-fat diet. Withdrawal of dietary phytoestrogens increased 3β-hydroxysteroid dehydrogenase and P450 aromatase gene expression and decreased those of 5α-reductase-1. This is a direct effect of the lack of dietary phytoestrogens and not a consequence of obesity, as it was not observed in high-fat–fed rats. Phytoestrogen withdrawal and high-fat diet intake reduced hypothalamic expression of estrogen receptor (ER)α correlated with low levels of ERα-O, ERα-OS, and ERα-OT transcripts. Variations in gene expression of steroidogenic enzymes may affect the content of neurosteroids. As neurosteroids are related to food intake control, the changes observed may be a novel mechanism in the regulation of energy balance in obese phytoestrogen-deprived animals. In rats, steroidogenesis and ER signaling appear to be altered by phytoestrogen withdrawal in the rat. The ubiquity of phytoestrogens in the diet and changing intakes or withdrawal suggest that aspects of human health could be affected based on the rat and warrant further research. Fil: Andreoli, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Stoker, Cora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Rossetti, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Lazzarino, Gisela Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Ramos, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f441875b62b458e97673ea146d086fd7Test
https://doi.org/10.1016/j.nutres.2016.05.003Test -
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المؤلفون: María Florencia Rossetti, Rocio Schumacher, Jorgelina Varayoud, Ayelen L. Gomez, Gisela Paola Lazzarino, Jorge G. Ramos
مصطلحات موضوعية: 0301 basic medicine, Aldosterone synthase, medicine.medical_specialty, 030209 endocrinology & metabolism, Biochemistry, Hippocampus, SENSORY ENRICHMENT, Epigenesis, Genetic, Running, Ciencias Biológicas, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, 3-Oxo-5-alpha-Steroid 4-Dehydrogenase, Internal medicine, Gene expression, medicine, Animals, Cytochrome P-450 CYP11B2, Epigenetics, Rats, Wistar, Promoter Regions, Genetic, Molecular Biology, Gene, DNA METHYLATION, Environmental enrichment, biology, Cytochrome P450, Steroid 17-alpha-Hydroxylase, STEROIDOGENIC ENZYMES, Methylation, DNA Methylation, Bioquímica y Biología Molecular, 3-alpha-Hydroxysteroid Dehydrogenase (B-Specific), Housing, Animal, MOTOR ENRICHMENT, Biosynthetic Pathways, Rats, Up-Regulation, 030104 developmental biology, DNA methylation, biology.protein, HIPPOCAMPUS, Female, Photic Stimulation, CIENCIAS NATURALES Y EXACTAS
الوصف: In the present study, we analyzed the effects of a short-term environmental enrichment on the mRNA expression and DNA methylation of steroidogenic enzymes in the hippocampus. Thus, young adult (80-day-old) and middle-aged (350-day-old) Wistar female rats were exposed to sensory (SE) or motor (ME) enrichment during 10 days and compared to animals housed under standard conditions. SE was provided by an assortment of objects that included plastic tubes and toys; for ME, rodent wheels were provided. In young adult animals, SE and ME increased the mRNA expression of cytochrome P450 17α-hydroxylase/c17,20-lyase, steroid 5α-reductase type 1 (5αR-1) and 3α-hydroxysteroid dehydrogenase and decreased the methylation levels of 5αR-1 gene. In middle-aged rats, ME and SE upregulated the gene expression of aldosterone synthase and decreased the methylation state of its promoter. These results propose that SE and ME differentially regulate the transcription of neurosteroidogenic enzymes through epigenetic mechanisms in young and aged rats. Fil: Rossetti, María Florencia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Schumacher, Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Varayoud, Jorgelina Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Fisiología Humana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Ramos, Jorge Guillermo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bed3f86a529a855ecef60c8ffc799af8Test
https://www.sciencedirect.com/science/article/pii/S0303720719300371?via=ihubTest -
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المؤلفون: Maria Florencia Andreoli, Marlise Guerrero-Schimpf, María Paula Gastiazoro, Oliver Zierau, Jorgelina Varayoud, Gisela Paola Lazzarino, Jorge G. Ramos, Enrique H. Luque, Milena Durando
مصطلحات موضوعية: 0301 basic medicine, Leptin, medicine.medical_specialty, CIENCIAS MÉDICAS Y DE LA SALUD, Endpoint Determination, medicine.medical_treatment, Uterus, Uterine hyperplasia, Estrogen receptor, Endocrine System, Biochemistry, UTERUS, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Internal medicine, medicine, Animals, Rats, Wistar, HYPERPLASIA, Molecular Biology, Cell Proliferation, ESTROGEN RECEPTOR, Leptin receptor, Hyperplasia, business.industry, Insulin, Body Weight, Estrogen Receptor alpha, Patología, Feeding Behavior, medicine.disease, Hormones, Endometrial hyperplasia, Diet, Medicina Básica, 030104 developmental biology, medicine.anatomical_structure, Adipose Tissue, 030220 oncology & carcinogenesis, Female, medicine.symptom, CAFETERIA DIET, business, ENDOMETRIAL PROLIFERATION
الوصف: Our aim was to evaluate whether chronic administration of CAF affects the uterus and induces the morphological and molecular changes associated with endometrial hyperplasia. Female Wistar rats exposed to CAF from weaning for 20 weeks displayed increased energy intake, body weight and fat depots, but did not develop metabolic syndrome. The adult uteri showed an increase in glandular volume fraction and stromal area. The epithelial proliferation rate and protein expression of oestrogen receptor alpha (ERα) were also increased. The CAF diet enhanced leptin serum levels and the long form of leptin receptor (Ob-Rb) mRNA expression in the uterus. No changes were detected in either insulin serum levels or those of insulin growth factor I (IGF-I) mRNA expression. However the levels of IGF-I receptor (IGF-IR) mRNA were lower in CAF-fed animals. Overall, the results indicate that our rat model of the CAF diet produces morphological and molecular changes associated with uterine hyperplasia and could predispose to endometrial carcinogenesis. Fil: Gastiazoro, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Guerrero Schimpf, Marlise Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Durando, Milena de Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Lazzarino, Gisela Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Andreoli, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Zierau, Oliver. Technische Universität Dresden; Fil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Ramos, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Varayoud, Jorgelina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57b6ed73bf1c56fad02a2a9dabf15546Test
https://linkinghub.elsevier.com/retrieve/pii/S030372071830193XTest -
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المؤلفون: María Florencia Rossetti, Jorge G. Ramos, Gisela Paola Lazzarino, Jorgelina Varayoud, Enrique H. Luque
المصدر: Molecular and Cellular Endocrinology. 429:73-83
مصطلحات موضوعية: 0301 basic medicine, Aldosterone synthase, Aging, Hippocampus, Biochemistry, MATERNAL EXPERIENCE, AGING, 0302 clinical medicine, Endocrinology, Pregnancy, Lactation, Gene expression, Promoter Regions, Genetic, DNA METHYLATION, Regulation of gene expression, Microfilament Proteins, Methylation, Bioquímica y Biología Molecular, medicine.anatomical_structure, NEUROSTEROIDOGENIC ENZYMES, DNA methylation, Female, Steroids, CIENCIAS NATURALES Y EXACTAS, medicine.medical_specialty, Synaptophysin, Nerve Tissue Proteins, Biology, Real-Time Polymerase Chain Reaction, Ciencias Biológicas, 03 medical and health sciences, Aromatase, Internal medicine, medicine, Animals, Computer Simulation, Cholesterol Side-Chain Cleavage Enzyme, RNA, Messenger, Rats, Wistar, Molecular Biology, Brain-Derived Neurotrophic Factor, Cholesterol side-chain cleavage enzyme, Promoter, DNA Methylation, Biosynthetic Pathways, 030104 developmental biology, Gene Expression Regulation, HIPPOCAMPUS, biology.protein, 030217 neurology & neurosurgery
الوصف: In the present study, we examined the mRNA expression and DNA methylation state of steroidogenic enzymes in the hippocampus of young adult (90-days-old) and middle-aged (450-days-old) nulliparous rats, and middle-aged multiparous rats subjected to three pregnancies with and without lactation. Aging decreased the mRNA levels of steroidogenic-related genes, while pregnancy and lactation significantly reduced the effect of aging, maintaining high expression levels of cytochrome P450 side-chain cleavage (P450scc), steroid 5α-reductase-1 (5αR-1), cytochrome P450arom (P450arom) and aldosterone synthase (P450(11β)-2). In addition, pregnancy and lactation diminished the methylation state of the 5αR-1 promoter and increased the transcription of brain-derived neurotrophic factor, synaptophysin and spinophilin. Pregnancy without lactation increased P450scc and 5αR-1 gene expression and decreased the methylation of their promoters. We concluded that the age-related decrease in the mRNA expression of steroidogenic enzymes is differentially attenuated by pregnancy and lactation in the rat hippocampus and that differential methylation mechanisms could be involved. Fil: Rossetti, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Varayoud, Jorgelina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Lazzarino, Gisela Paola. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Ramos, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::223f07ea11099668deb30584018f3ba1Test
https://doi.org/10.1016/j.mce.2016.03.037Test
