المؤلفون: Alessandro Garcia Lopes, Gildásio Castello de Almeida Júnior, Ronan Marques Teixeira, Luiz Carlos de Mattos, Cinara Cássia Brandão de Mattos, Lilian Castiglioni

المصدر: BMC Research Notes, Vol 13, Iss 1, Pp 1-7 (2020)

مصطلحات موضوعية: Keratoconus, Cornea, Superoxide Dismutase-1 gene, Corneal Diseases, Polymorphism, Genetic, Medicine, Biology (General), QH301-705.5, Science (General), Q1-390

الوصف: Abstract Objective To determine the presence of the 7-bp deletion c.169+50delTAAACAG in intron 2 of Superoxide Dismutase-1 gene in keratoconic patients from the State of São Paulo, Brazil, which promotes splicing variations, resulting in non-functional Superoxide Dismutase-1 antioxidant proteins, which may damage the corneal structure. Results A group of 35 keratoconic patients, from whom 35 peripheral blood samples and 58 samples of corneal fragments were evaluated, and a control group of 89 individuals, from whom 41 blood samples and 149 samples of corneal fragments were collected. After the amplification of DNA fragments by polymerase chain reaction, mutational screening analysis was performed by enzymatic digestion, followed by direct sequencing. The absence of the 7-bp c.169+50delTAAACAG mutation in intron 2 of Superoxide Dismutase-1 gene was detected in the analyzed subjects of the 2 groups, both in the cornea and peripheral blood samples. Then, according to our results, there is no involvement of c.169+50delTAAACAG deletion in the pathogenesis of keratoconus in this population, once it was not detected. But we emphasize that studies involving this deletion must be continued in an attempt to elucidate this issue.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s13104-020-05166-3Test; https://doaj.org/toc/1756-0500Test

الوصول الحر: https://doaj.org/article/dc66ac4a1b884284bb734df4fd46cc6eTest

المؤلفون: Amanda Pires Barbosa, Gildásio Castello de Almeida Júnior, Marta Ferrari Teixeira, José Carlos Barbosa

المصدر: Revista Brasileira de Oftalmologia, Vol 71, Iss 6, Pp 353-357 (2012)

مصطلحات موضوعية: Transplante de córnea, Ceratoplastia penetrante, Doenças da córnea, Opacidade da córnea, Estudos retrospectivos, Ophthalmology, RE1-994

الوصف: OBJETIVO: Determinar os diagnósticos clínicos corneanos na indicação da Ceratoplastia Penetrante (CP) de pacientes inscritos no Banco de Olhos do Hospital de Base de São José do Rio Preto, correlacionando-os com outras variáveis, como a faixa etária, sexo e situação sócioeconômica. MÉTODOS: Realizou-se análise retrospectiva de 1085 fichas de inscrição de pacientes submetidos à Ceratoplastia Penetrante, no Hospital de Base de São José do Rio Preto, no período de 2000 a 2009. Os diagnósticos para indicação das cirurgias foram posteriormente correlacionados com a faixa etária, o sexo e o nível socioeconômico dos pacientes, por meio do teste do Qui-quadrado. RESULTADOS: Do total de Ceratoplastias Penetrantes, 57,4% foram realizadas em pacientes do sexo masculino, e a média de idade encontrada foi de 52,3 anos (DP=21,42). Verificou-se que em 90,8% dos casos a cirurgia ocorreu por meio do Sistema Único de Saúde (SUS), enquanto nos demais (9,2%) os pacientes possuíam convênio ou custearam todo o procedimento. A indicação mais frequentemente encontrada foi o leucoma (23%), o qual ocorreu em 14% das CPs realizadas em homens e em 9% das ocorridas em mulheres. Em relação às CPs com este diagnóstico, 40% ocorreram em pacientes com idade superior a 60 anos, com porcentagem decrescendo com as faixas etárias mais jovens, e 94,4% efetuaram-se através do SUS, enquanto que apenas 5,6% dos casos se encaixaram no quesito convênio/particular. CONCLUSÃO: A principal indicação à Ceratoplastia Penetrante encontrada foi o leucoma, o qual predominou em homens, nas idades acima de 60 anos, em pacientes do SUS.

وصف الملف: electronic resource

العلاقة: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-72802012000600002&lng=en&tlng=enTest; https://doaj.org/toc/1982-8551Test

الوصول الحر: https://doaj.org/article/228cc1ed8d03433bb3f1bce43281f9e6Test

المؤلفون: Gildásio Castello de Almeida Júnior, Ronan Marques Teixeira, Alessandro Garcia Lopes, Lilian Castiglioni, Cinara Cássia Brandão de Mattos, Luiz Carlos de Mattos

المساهمون: Universidade Estadual Paulista (Unesp), Faculdade de Medicina de São José Do Rio Preto (FAMERP), Fundação Faculdade Regional de Medicina (HB-FUNFARME)

المصدر: BMC Research Notes
BMC Research Notes, Vol 13, Iss 1, Pp 1-7 (2020)
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP

مصطلحات موضوعية: 0301 basic medicine, Male, lcsh:Medicine, medicine.disease_cause, Polymerase Chain Reaction, law.invention, Corneal Diseases, Cornea, 0302 clinical medicine, Superoxide Dismutase-1, law, lcsh:QH301-705.5, Polymerase chain reaction, Sequence Deletion, education.field_of_study, Mutation, General Medicine, Middle Aged, Research Note, medicine.anatomical_structure, Female, Brazil, Polymorphism, Restriction Fragment Length, Adult, Keratoconus, Population, SOD1, Superoxide Dismutase-1 gene, Biology, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Young Adult, Genetic, medicine, Humans, Polymorphism, education, lcsh:Science (General), Gene, lcsh:R, Intron, Sequence Analysis, DNA, medicine.disease, Molecular biology, Introns, 030104 developmental biology, lcsh:Biology (General), 030221 ophthalmology & optometry, lcsh:Q1-390

الوصف: Made available in DSpace on 2020-12-12T02:14:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-07-09 Objective: To determine the presence of the 7-bp deletion c.169+50delTAAACAG in intron 2 of Superoxide Dismutase-1 gene in keratoconic patients from the State of São Paulo, Brazil, which promotes splicing variations, resulting in non-functional Superoxide Dismutase-1 antioxidant proteins, which may damage the corneal structure. Results: A group of 35 keratoconic patients, from whom 35 peripheral blood samples and 58 samples of corneal fragments were evaluated, and a control group of 89 individuals, from whom 41 blood samples and 149 samples of corneal fragments were collected. After the amplification of DNA fragments by polymerase chain reaction, mutational screening analysis was performed by enzymatic digestion, followed by direct sequencing. The absence of the 7-bp c.169+50delTAAACAG mutation in intron 2 of Superoxide Dismutase-1 gene was detected in the analyzed subjects of the 2 groups, both in the cornea and peripheral blood samples. Then, according to our results, there is no involvement of c.169+50delTAAACAG deletion in the pathogenesis of keratoconus in this population, once it was not detected. But we emphasize that studies involving this deletion must be continued in an attempt to elucidate this issue. Biology Department Instituto de Biociências Letras e Ciências Exatas IBILCE-UNESP Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Cristóvão Colombo, 2265 Immunogenetics Laboratory Molecular Biology Department Faculdade de Medicina de São José Do Rio Preto (FAMERP), Avenida Brigadeiro Faria Lima, 5416, Vila São Pedro Ophthalmology Outpatient Clinic Hospital de Base de São José Do Rio Preto Fundação Faculdade Regional de Medicina (HB-FUNFARME), Avenida Brigadeiro Faria Lima, 5544 Epidemiology and Health Department Faculdade de Medicina de São José Do Rio Preto (FAMERP), Avenida Brigadeiro Faria Lima, 5416, Vila São Pedro Biology Department Instituto de Biociências Letras e Ciências Exatas IBILCE-UNESP Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Cristóvão Colombo, 2265

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35c3b567ce586bf2a427d1036df1999bTest
https://pubmed.ncbi.nlm.nih.gov/32646478Test

المؤلفون: Luiz Carlos de Mattos, Fernando H.A. Murata, Aparecida Perpétuo Silveira-Carvalho, Alessandro Garcia Lopes, Amanda Pires Barbosa, Rubens Camargo Siqueira, Christiane Maria Ayo, Fábio Batista Frederico, Cinara Cássia Brandão de Mattos, Mariana Previato, Amanda Priscila de Oliveira, Geraldo Magela de Faria Junior, Gildásio Castello de Almeida Júnior

المساهمون: Faculdade de Medicina de São José do Rio Preto (FAMERP), Ophthalmology Outpatient Clinic of Hospital de Base da Fundação Faculdade Regional de Medicina de São José do Rio Preto (HB– FUNFARME), Universidade Estadual Paulista (Unesp), Medical Specialties Outpatient Clinic of Hospital Estadual 'João Paulo II' (AME)

المصدر: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP

مصطلحات موضوعية: Male, 0301 basic medicine, Aging, Chemokine receptor CCR5, Ocular toxoplasmosis, CCR5 receptor, Polymerase Chain Reaction, Gastroenterology, Serology, Chemokine receptor, 0302 clinical medicine, Risk Factors, Genotype, Aged, 80 and over, biology, Middle Aged, Infectious Diseases, Female, Chemokines, Toxoplasma, Polymorphism, Restriction Fragment Length, Adult, medicine.medical_specialty, Adolescent, Receptors, CCR5, Veterinary (miscellaneous), Toxoplasma gondii, Young Adult, 03 medical and health sciences, Internal medicine, medicine, Humans, Genetic Predisposition to Disease, Toxoplasmosis, Ocular, Aged, Genetic polymorphism, Polymorphism, Genetic, business.industry, biology.organism_classification, medicine.disease, Chemokine Receptor Gene, Toxoplasmosis, 030104 developmental biology, Case-Control Studies, Insect Science, 030221 ophthalmology & optometry, biology.protein, Parasitology, CC chemokine receptors, business

الوصف: Made available in DSpace on 2018-12-11T17:16:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-02-01 C–C chemokine receptor type 5 (CCR5) is a chemokine receptor that influences the immune response to infectious and parasitic diseases. This study aimed to determine whether the CCR5Δ32 and CCR5 59029 A/G polymorphisms are associated with the development of ocular toxoplasmosis in humans. Patients with positive serology for Toxoplasma gondii were analyzed and grouped as ‘with ocular toxoplasmosis’ (G1: n = 160) or ‘without ocular toxoplasmosis’ (G2: n = 160). A control group (G3) consisted of 160 individuals with negative serology. The characterization of the CCR5Δ32 and CCR5 59029 A/G polymorphisms was by PCR and by PCR-RFLP, respectively. The difference between the groups with respect to the mean age (G1: mean age: 47.3, SD ± 19.3, median: 46 [range: 18–95]; G2: mean age: 61.3, SD ± 13.7, median: 61 [range: 21–87]; G3: mean age: 38.8, SD ± 17.9, median: 34 [range: 18–80]) was statistically significant (G1 vs.G2: p-value

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a9cf6e1d7611a1ed5b398f8e6db5ca0Test
https://doi.org/10.1016/j.actatropica.2017.12.012Test

المؤلفون: Margarete Teresa Gottardo de Almeida, Leandro Fernandes Coelho, Maicon Santos Gonçalves, Gildásio Castello de Almeida Júnior, Vinicius Targa Villas Bôas

المصدر: Arquivos Brasileiros de Oftalmologia v.81 n.5 2018
Arquivos brasileiros de oftalmologia
Conselho Brasileiro de Oftalmologia (CBO)
instacron:CBO
Arquivos Brasileiros de Oftalmologia, Vol 81, Iss 5, Pp 371-375

مصطلحات موضوعية: Microbiological culture, Contact Lenses, Fungal contamination, Lentes de contato, Statistical difference, Dentistry, Lens (geology), Gram-Positive Bacteria, Keratitis, 03 medical and health sciences, Hospital, 0302 clinical medicine, lcsh:Ophthalmology, Surveys and Questionnaires, Gram-Negative Bacteria, medicine, Humans, Contact lenses, business.industry, Potential risk, Microbiota, Fungi, Ceratite, General Medicine, Eye infection, medicine.disease, Contact lens, Ophthalmology, lcsh:RE1-994, 030221 ophthalmology & optometry, Equipment Contamination, business, 030217 neurology & neurosurgery

الوصف: Purpose: The aim of this study was to analyze the bacterial and fungal microbiota found in contact lens cases among two groups of patients to correlate the data on the habits of contact lens users and to evaluate whether there is a difference in the culture results between users of ridged and nonridged contact lens cases. Methods: Two groups (35 patients per group) were included, consisting of hospital workers and those who had not visited a hospital in the past 30 days, and a questionnaire regarding epidemiological data and habits related to contact lens and lens case use was administered. In addition, 140 samples collected from the right and left compartments of each lens case by swabbing the bottom of the wells were tested using bacterioscopy as well as fungal and bacterial cultures via computerized identification of the species. Results: No fungal growth was identified in any of the 70 contact lens cases; however, bacteria were found in 39 cases, and there was no statistical difference between the groups. Most bacteria (>85%) were gram-negative bacilli. Contamination inone compartment of the contact lens case elevated the risk of contamination of the other side (>80%). Moreover, contamination was statistically higher in the ridged cases than in nonridged cases (p=0.0149). Conclusion: The types of bacteria contaminating the cases are generally not seen in eye diseases associated with contact lens use, suggesting that other decisive variables are involved in eye infection from a contaminated lens or case. Fungal contamination of contact lens cases appears to be an exception. Ridged cases are commonly used by contact lens wearers and present a potential risk to eye health. In addition, the results of bacterial tests between hospital workers and those who did not visit a hospital were not significantly different.

وصف الملف: text/html

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d04f189eb81fead4d132a1903158920Test
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492018000500371Test

المؤلفون: Afonso Kamimura, Magali Ineko Takata, Ana Carolina Moraes Fernandes, João Paulo Neves, Marco Túlio Chater Viegas, Valquise Yumi Murata, Maurício Lacerda Nogueira, Gildásio Castello de Almeida Júnior

المصدر: Arquivos Brasileiros de Oftalmologia, Vol 71, Iss 6, Pp 827-830 (2008)

مصطلحات موضوعية: Córnea, Keratitis, Cornea, Herpes simplex, Keratitis, herpetic, lcsh:Ophthalmology, lcsh:RE1-994, Reação em cadeia da polimerase, Ceratite, Herpes simples, Ceratite herpética, Polymerase chain reaction

الوصف: OBJETIVO: Avaliação dos pacientes com o quadro clínico de ceratite herpética (CH) típicas e atípicas, pela reação em cadeia da polimerase (PCR) correlacionando com o diagnóstico clínico. MÉTODOS: Foi realizada a PCR em 28 pacientes com ceratite herpética típica e atípica. RESULTADOS: A PCR foi positiva em 57,14% (n=16) do total (n=28). Nos casos de CH típica a positividade foi de 60,00% (n=12) em 20 casos. Para CH epitelial a positividade foi de 69,23% (n=9), sendo 77,78% (n=7) apenas para as lesões epiteliais dendríticas. Os casos de CH atípica apresentaram positividade de 50% (n=4) em oito casos. CONCLUSÃO: Quadro clínico típico de CH teve boa correlação com o resultado positivo observado na PCR. Entretanto, metade dos pacientes com o quadro de CH atípica apresentou PCR positivo, portanto, o exame do PCR é teste importante para o auxílio e diagnóstico da CH. No caso de CH estromal, foi demonstrado que a técnica da PCR conseguiu identificar o vírus HSV.PURPOSE: To evaluate patients with clinically typical and atypical herpetic keratitis (HK) by means of polymerase chain reaction (PCR) as compared with the clinical diagnosis. METHODS: PCR in 28 patients with clinical symptoms of typical and atypical HK was performed. RESULTS: PCR was positive in 57.14% (n=16) of the total cases (n=28). The test was positive in 60.0% (n=12) of the 20 typical HK cases. For epithelial HK, the test was positive in 69.23% (n=9), and 77.78% (n=7) only for dendritic injuries. Atypical HK presented a positive test in 50% (n=4) of eight cases. CONCLUSION: Clinical typical picture of HK had a good correlation with the positive result of PCR, mainly for epithelial injury of the dendritic type. However, 50% of the patients with atypical HK presented positive PCR. This result showed that PCR test can provide an effective HK diagnosis. In the stromal case of HK, PCR was a useful technique to identify HSV virus.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doajarticles::71b3d696adda3ab429d3e48ba92ce224Test
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492008000600012Test

المؤلفون: Reinaldo Azoubel, Gildásio Castello de Almeida Júnior, Patrícia Maluf Cury, Luciano Arakawa, Milton Ruiz Alves, Dalisio de Santi Neto, Acácio Alves de Souza Lima Filho, Sidney Júlio de Faria e Sousa

المصدر: Arquivos Brasileiros de Oftalmologia, Volume: 78, Issue: 1, Pages: 1-5, Published: FEB 2015
Arquivos Brasileiros de Oftalmologia, Vol 78, Iss 1, Pp 1-5 (2015)
Arquivos Brasileiros de Oftalmologia v.78 n.1 2015
Arquivos brasileiros de oftalmologia
Conselho Brasileiro de Oftalmologia (CBO)
instacron:CBO

مصطلحات موضوعية: Male, Conjunctiva/ surgery, Anti-allergic agents/therapeutic use, Pterygium, law.invention, Randomized controlled trial, lcsh:Ophthalmology, law, Transforming Growth Factor beta, Recurrence, Secondary Prevention, Medicine, ortho-Aminobenzoates, Postoperative Period, Prospective Studies, Prospective cohort study, Autografts, biology, Antialérgicos/uso terapêutico, General Medicine, Middle Aged, Treatment Outcome, Pterygium/therapy, Transplantation, autologous, Granuloma, Fator de crescimento transformador beta 1, Female, Injections, Intraocular, Conjunctiva, medicine.drug, Adult, medicine.medical_specialty, Tranilast, Mitomycin, Transplante autólogo, Urology, Fibrin Tissue Adhesive, Preoperative care, Transplantation, Autologous, Fibrin, Preoperative Care, Humans, Pterígio/terapia, Autoenxertos, business.industry, Mitomycin C, Epithelial Cells, medicine.disease, Transplantation, Ophthalmology, Túnica conjuntiva/cirurgia, lcsh:RE1-994, biology.protein, Transforming growth factor beta 1, business, Follow-Up Studies, Recidiva

الوصف: Purpose: To determine the efficacy of tranilast as an adjunctive therapy in conjunctival autograft. Methods: Twenty-nine patients were randomly allocated to the Tranilast Group (n=15) or the Control Group (n=14). The Tranilast Group received a subconjunctival injection of 0.5% tranilast 30 days prior to surgery. Conjunctival autograft was performed in both groups using fibrin sealant and 0.02% subconjunctival mitomycin C at the end of the surgery. After the resection of the pterygium, immunohistochemistry was performed with 100 cells to identify epithelial cells positive for transforming growth factor-β (TGF-β). Subjective symptoms were evaluated using a 5-point scale, and the recurrence rate was assessed. Results: Both groups showed improvements in their symptoms and similar clinical results. Compared with the Control Group, the Tranilast Group failed to show a decreased recurrence rate (p=0.59). However, the number of epithelial cells expressing TGF-β was lower in the Tranilast Group (5 cells; 95% CI: 2.56-13.15; Control Group, 16 cells, 95% CI: 11.53-24.76; p=0.01). Minimal but reversible complications, including glaucoma secondary to corticosteroids and granuloma, occurred during the study. Conclusion: Tranilast was effective in decreasing the number of pterygium epithelial cells expressing TGF-β. Objetivo: Determinar a eficácia do tranilast, como terapia auxiliar no transplante autólogo de conjuntiva. Métodos: Vinte e nove pacientes foram randomizados em dois grupos: Grupo Tratado (15) e Grupo Controle (14). Trinta dias antes da cirurgia, o Grupo Tratado recebeu uma injeção subconjuntival de tranilast a 0,5%. O transplante autólogo de conjuntiva foi realizado em ambos os grupos, usando-se a cola de fibrina e a mitomicina 0,02% subconjuntival, ao final da cirurgia. Cada paciente foi examinado por 12 meses de acompanhamento. A imuno-histoquímica foi realizada, mediante um total de 100 células, a fim de que se contassem as células epiteliais positivas, para o fator de crescimento transformador beta (TGF-β), após a cirurgia do pterígio. Os sintomas subjetivos foram avaliados usando-se uma escala de cinco pontos, e a taxa de recorrência foi avaliada. Resultados: Os 2 grupos apresentaram melhora dos sintomas e com resultados clínicos similares. Quando comparado com o Grupo Controle, o Grupo Tratado falhou em mostrar uma diminuição da taxa de recorrência (p=0,59). Entretanto o número de células epiteliais expressando o TGF-β foi menor no Grupo Tratado (5 células; 95% CI=2,56-13,15; Grupo Controle, 16 células; 95% CI: 11,53-24,76, p=0,01). Complicações mínimas, mas reversíveis, ocorreram durante o estudo, incluindo glaucoma secundário ao uso de corticoide e granuloma. Conclusão: O tranilast foi efetivo em diminuir o número células epiteliais do pterígio expressando o TGF-β.

وصف الملف: text/html

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5bf9a63be407685054a0b0ad9e4821baTest
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492015000100002&lng=en&tlng=enTest

المؤلفون: Maurício Lacerda Nogueira, Marco Túlio Chater Viegas, Ana Carolina Moraes Fernandes, Gildásio Castello de Almeida Júnior, Afonso Kamimura, Magali Ineko Takata, João Paulo Neves, Valquise Yumi Murata

المصدر: Arquivos Brasileiros de Oftalmologia, Volume: 71, Issue: 6, Pages: 827-830, Published: DEC 2008
Arquivos Brasileiros de Oftalmologia v.71 n.6 2008
Arquivos brasileiros de oftalmologia
Conselho Brasileiro de Oftalmologia (CBO)
instacron:CBO

مصطلحات موضوعية: Pathology, medicine.medical_specialty, Herpes simplex, Reação em cadeia da polimerase, Herpes simples, medicine.disease_cause, Virus, Keratitis, law.invention, Cornea, Pcr test, law, medicine, In patient, Positive test, Ceratite herpética, Polymerase chain reaction, business.industry, General Medicine, Ceratite, medicine.disease, Córnea, Ophthalmology, Herpes simplex virus, Keratitis, herpetic, Clinical diagnosis, business

الوصف: OBJETIVO: Avaliação dos pacientes com o quadro clínico de ceratite herpética (CH) típicas e atípicas, pela reação em cadeia da polimerase (PCR) correlacionando com o diagnóstico clínico. MÉTODOS: Foi realizada a PCR em 28 pacientes com ceratite herpética típica e atípica. RESULTADOS: A PCR foi positiva em 57,14% (n=16) do total (n=28). Nos casos de CH típica a positividade foi de 60,00% (n=12) em 20 casos. Para CH epitelial a positividade foi de 69,23% (n=9), sendo 77,78% (n=7) apenas para as lesões epiteliais dendríticas. Os casos de CH atípica apresentaram positividade de 50% (n=4) em oito casos. CONCLUSÃO: Quadro clínico típico de CH teve boa correlação com o resultado positivo observado na PCR. Entretanto, metade dos pacientes com o quadro de CH atípica apresentou PCR positivo, portanto, o exame do PCR é teste importante para o auxílio e diagnóstico da CH. No caso de CH estromal, foi demonstrado que a técnica da PCR conseguiu identificar o vírus HSV. PURPOSE: To evaluate patients with clinically typical and atypical herpetic keratitis (HK) by means of polymerase chain reaction (PCR) as compared with the clinical diagnosis. METHODS: PCR in 28 patients with clinical symptoms of typical and atypical HK was performed. RESULTS: PCR was positive in 57.14% (n=16) of the total cases (n=28). The test was positive in 60.0% (n=12) of the 20 typical HK cases. For epithelial HK, the test was positive in 69.23% (n=9), and 77.78% (n=7) only for dendritic injuries. Atypical HK presented a positive test in 50% (n=4) of eight cases. CONCLUSION: Clinical typical picture of HK had a good correlation with the positive result of PCR, mainly for epithelial injury of the dendritic type. However, 50% of the patients with atypical HK presented positive PCR. This result showed that PCR test can provide an effective HK diagnosis. In the stromal case of HK, PCR was a useful technique to identify HSV virus.

وصف الملف: text/html

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb3b84b123505354d8ff9040d96140ecTest
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492008000600012&lng=en&tlng=enTest

المؤلفون: Gildásio Castello de Almeida Júnior, Ângela Yuri Iquejiri, Tatiana Vanucci Garcia, Fábio Batista Frederico, Patrícia Maluf Cury, Karina Paula Watanabe, Reinaldo Azoubel, Sebastião Roberto Taboga

المساهمون: Faculdade de Medicina de São José do Rio Preto (FAMERP), Universidade Estadual Paulista (Unesp)

المصدر: Arquivos Brasileiros de Oftalmologia v.71 n.4 2008
Arquivos brasileiros de oftalmologia
Conselho Brasileiro de Oftalmologia (CBO)
instacron:CBO
SciELO
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Arquivos Brasileiros de Oftalmologia, Volume: 71, Issue: 4, Pages: 568-575, Published: AUG 2008

مصطلحات موضوعية: Pathology, Karyometry, Mitomycin/therapeutic use, Epithelial cells, Antígeno Ki-67, Cariometria, Pterygium, Corneal Transplantation, Recurrence, Secondary Prevention, Ki-67 antigen, Células epiteliais, Pterygium (conjunctiva), biology, General Medicine, Middle Aged, medicine.anatomical_structure, Treatment Outcome, Immunohistochemistry, Conjunctiva, Adult, medicine.medical_specialty, Antimetabolites, Antineoplastic, Mitomicina, Mitomycin, Pterygium/drug therapy, Statistics, Nonparametric, Antigen, Proliferating Cell Nuclear Antigen, medicine, Humans, Fibroblast, Cell Proliferation, business.industry, Mitomycin C, Epithelial Cells, Fibroblasts, medicine.disease, Fibroblastos, Proliferating cell nuclear antigen, Transplantation, Fibroblast Growth Factors, Ophthalmology, Ki-67 Antigen, Pterígio, biology.protein, Ophthalmic Solutions, business, Recidiva

الوصف: Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:46:10Z No. of bitstreams: 1 S0004-27492008000400019.pdf: 379652 bytes, checksum: 0f2c62bcfe155e5e888c250dbb1835c9 (MD5) Made available in DSpace on 2013-08-22T18:46:10Z (GMT). No. of bitstreams: 1 S0004-27492008000400019.pdf: 379652 bytes, checksum: 0f2c62bcfe155e5e888c250dbb1835c9 (MD5) Previous issue date: 2008-08-01 Made available in DSpace on 2013-09-30T19:35:21Z (GMT). No. of bitstreams: 2 S0004-27492008000400019.pdf: 379652 bytes, checksum: 0f2c62bcfe155e5e888c250dbb1835c9 (MD5) S0004-27492008000400019.pdf.txt: 42035 bytes, checksum: 2c6a87402ed8ff8cfe340ad549027522 (MD5) Previous issue date: 2008-08-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:09:32Z No. of bitstreams: 2 S0004-27492008000400019.pdf: 379652 bytes, checksum: 0f2c62bcfe155e5e888c250dbb1835c9 (MD5) S0004-27492008000400019.pdf.txt: 42035 bytes, checksum: 2c6a87402ed8ff8cfe340ad549027522 (MD5) Made available in DSpace on 2014-05-20T15:09:32Z (GMT). No. of bitstreams: 2 S0004-27492008000400019.pdf: 379652 bytes, checksum: 0f2c62bcfe155e5e888c250dbb1835c9 (MD5) S0004-27492008000400019.pdf.txt: 42035 bytes, checksum: 2c6a87402ed8ff8cfe340ad549027522 (MD5) Previous issue date: 2008-08-01 OBJETIVO: Avaliar a eficácia da mitomicina C (MMC) na prevenção da recorrência quando previamente utilizada no transplante autólogo de conjuntiva (TAC). A avaliação da proliferação celular epitelial pelo antígeno Ki-67 e a cariometria do núcleo dos fibroblastos foram usados como auxiliares na avaliação do tratamento. MÉTODOS: Vinte e nove pacientes com pterígio recidivado foram divididos em três grupos: Grupo (G) 1-TAC e colírio placebo (PLA); G2-TAC, MMC 0,015% subconjuntival e PLA; G3-TAC e colírio de MMC 0,02%. A imuno-histoquímica foi realizada no tecido excisado para o antígeno Ki-67, como a cariometria dos núcleos dos fibroblastos (divididos em lado nasal e temporal). A cariometria dos núcleos dos fibroblastos foi avaliada de acordo com os seguintes parâmetros: volume (Vl) e área (Ar) em pelos menos 50 células por paciente. RESULTADOS: A porcentagem das células epiteliais positivas para o antígeno Ki-67 no lado nasal e temporal após o tratamento dos três grupos estudados foi: nasal (3,30% G1, 4,49% G2 e 3,38% G3) e temporal (3,30% G1, 4,46% G2 e 4,14% G3) não mostrando diferença significativa. A cariometria do núcleo dos fibroblastos foi: Vl nasal (792,1 µ3 G1, 605,1 µ3 G2, e 549,9 µ3 G3) e a Ar (100,58 µ2 G1, 83,13 µ2 G2, e 78,41 µ2 G3). Os três grupos mostraram uma diferença significativa p=0,039 e p=0,035, respectivamente do Vl e da Ar no lado nasal. Após seis meses de tratamento, os três grupos apresentaram a seguinte taxa de recidiva: 22,22% G1, 18,18%, G2 e 33,33% G3 respectivamente. CONCLUSÃO: O uso da MMC não interferiu nas células epiteliais positivas para o antígeno Ki-67 no pterígio recidivado, mas acarretou diminuição do volume e área dos núcleos dos fibroblastos no lado nasal do pterígio. As células epiteliais positivas para o antígeno Ki-67 parecem não ter relação com a recidiva do pterígio após seis meses da cirurgia. Outros estudos devem ser realizados para avaliar o papel da proliferação das células epiteliais na recorrência do pterígio. PURPOSE: To evaluate the effectiveness of mitomycin C (MMC) in preventing recurrence of pterygium following conjunctival autograft transplantation (CAT). Ki-67 antigen to evaluate epithelial cell proliferation and fibroblast nuclear kariometry were used to assist treatment evaluation. METHODS: Twenty-nine patients with recurrent pterygium were divided into three groups: Group (G) 1 - CAT and placebo eyedrops (PED); G2 - CAT, 0.015% MMC subconjunctivally, and PED; G3 - CAT and 0.02% MMC eyedrops. Immunohistochemistry for the Ki-67 antigen and fibroblast nuclei kariometry were performed on the excised tissue, divided into nasal and temporal sides. Kariometry was evaluated in terms of volume (Vl) and area (Ar) using at least 50 cells/patient. RESULTS: The percentage of positive epithelial cells for the Ki-67 antigen on the nasal and temporal side after treatment of the three groups were: nasal (5.39% G1, 4.49% G2, and 3.88% G3); temporal (3.30% G1, 4.46% G2, 4.14% G3), did not show significant differences. Fibroblast nucleus kariometry was: nasal Vl (792.1 µ3 G1, 605.1 µ3 G2, and 549.9 µ3 G3) and Ar (100.58 µ2 G1, 83.13 µ2 G2, and 78.41 µ2 G3). The three groups showed significant differences: p=0.039 and p=0.035, for respectively Vl and Ar, on the nasal side. After a six month of treatment, the three groups presented the following recurrence rates: G1, 22.22%, G2, 18.18% and G3, 33.33%, respectively. CONCLUSION: MMC did not reduce the number of positive epithelial cells for the Ki-67 antigen in recurrent pterygium, but decreased fibroblast nucleus volume and area on the nasal side of the pterygia. The number of positive epithelial cells for the Ki-67 antigen seemed not to be related to pterygium recurrence observed over a six-month post-surgery period. The role of epithelial cell proliferation in pterygium recurrence should be evaluated by further studies. Faculdade de Medicina de São José do Rio Preto (FAMERP) FAMERP FAMERP Departamento de Patologia Universidade Estadual Paulista Júlio de Mesquita Filho Departamento de Biologia Universidade Estadual Paulista Júlio de Mesquita Filho Departamento de Biologia

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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492008000400019Test

المؤلفون: Luiz Kazuo Kashiwabuchi, Gildásio Castello de Almeida Júnior, Gustavo Nishimura Aragaki, Marta Ferrari Teixeira, Eliane Terumi Inada

المصدر: Arquivos Brasileiros de Oftalmologia, Volume: 66, Issue: 4, Pages: 473-476, Published: AUG 2003
Arquivos Brasileiros de Oftalmologia, Vol 66, Iss 4, Pp 473-476 (2003)
Arquivos Brasileiros de Oftalmologia v.66 n.4 2003
Arquivos brasileiros de oftalmologia
Conselho Brasileiro de Oftalmologia (CBO)
instacron:CBO

مصطلحات موضوعية: Saúde ocular, Accidents occupational, Eye health, Acidentes ocupacionais, Eye injuries, Construction materials, General Medicine, Blindness, Traumatismos oculares, Materiais de construção, Ophthalmology, lcsh:Ophthalmology, lcsh:RE1-994, Cegueira, Traumatismos oculares/etiologia, Cegueira/prevenção & controle

الوصف: OBJETIVOS: Estudar a prevalência e as circunstâncias do trauma ocular grave em um Hospital Universitário de São José do Rio Preto - SP. MÉTODOS: Realizou-se estudo retrospectivo de 216 pacientes no período de setembro de 1986 a setembro de 2000 com traumatismo ocular grave que evoluiu para cirurgia. Foram avaliados o sexo, idade, lateralidade, causa do trauma, tipo de trauma, atividade no momento do acidente e acuidade visual pós-trauma. RESULTADOS: Foram estudados 216 pacientes sendo 173 (80%) do sexo masculino. A faixa etária mais acometida foi a menor de 20 anos em 94 (43,5%) pacientes. As lesões unilaterais predominaram em 209 (96,8%). A atividade predominante no momento do trauma foi o lazer em 88 (40,7%) pacientes e os principais agentes causais foram os materiais de construção em 82 (38%) dos casos. A perfuração ocular foi o tipo de trauma mais comum em 84 (85,2%) pacientes. CONCLUSÕES: As perfurações oculares unilaterais por materiais de construção predominaram em crianças, adolescentes e idosos do sexo masculino em atividades de lazer. PURPOSE: To study the prevalence and circumstances of severe ocular trauma in a University Hospital of São José do Rio Preto - SP. METHODS: A retrospective study was performed on 216 patients with severe ocular trauma who underwent surgery between September, 1986 to September, 2000. Sex, age, la-terality, cause of trauma, type of trauma, activity during the accident and final vision after trauma were evaluated. RESULTS: 216 patients of whom 173 (80%) were male, were studied. The most frequent age was below 20 years in 94 (43.5%) patients. The unilateral injuries predominated in 209 (96.8%). The major activity during the accident was leisure in 88 (40.7%) patients. The most frequent agent was construction materials in 82 (38%) cases. Ocular perforation was the most frequent type of trauma in 84 (85.2%) patients. CONCLUSIONS: Unilateral ocular perforations by construction materials predominated in male children, teenagers and old people during leisure.

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