المؤلفون: Muhammad Wasim Haider, Muhammad Nafees, Rashid Iqbal, Habat Ullah Asad, Farrukh Azeem, Muhammad Samsam Raza, Abdel-Rhman Z. Gaafar, Mohamed S. Elshikh, Muhammad Arslan, Muhammad Habib Ur Rahman, Ayman M. S. Elshamly

المصدر: BMC Plant Biology, Vol 24, Iss 1, Pp 1-15 (2024)

مصطلحات موضوعية: Acacia niloticaLinn., Colchicine, Enzymatic and non-enzymatic antioxidants, Germination percentage, Morphology, Stomatal index, Botany, QK1-989

الوصف: Abstract Background Acacia nilotica Linn. is a widely distributed tree known for its applications in post-harvest and medicinal horticulture. However, its seed-based growth is relatively slow. Seed is a vital component for the propagation of A. nilotica due to its cost-effectiveness, genetic diversity, and ease of handling. Colchicine, commonly used for polyploidy induction in plants, may act as a pollutant at elevated levels. Its optimal concentration for Acacia nilotica's improved growth and development has not yet been determined, and the precise mechanism underlying this phenomenon has not been established. Therefore, this study investigated the impact of optimized colchicine (0.07%) seed treatment on A. nilotica's morphological, anatomical, physiological, fluorescent, and biochemical attributes under controlled conditions, comparing it with a control. Results Colchicine seed treatment significantly improved various plant attributes compared to control. This included increased shoot length (84.6%), root length (53.5%), shoot fresh weight (59.1%), root fresh weight (42.8%), shoot dry weight (51.5%), root dry weight (40%), fresh biomass (23.6%), stomatal size (35.9%), stomatal density (41.7%), stomatal index (51.2%), leaf thickness (11 times), leaf angle (2.4 times), photosynthetic rate (40%), water use efficiency (2.2 times), substomatal CO2 (36.6%), quantum yield of photosystem II (13.1%), proton flux (3.1 times), proton conductivity (2.3 times), linear electron flow (46.7%), enzymatic activities of catalase (25%), superoxide dismutase (33%), peroxidase (13.5%), and ascorbate peroxidase (28%), 2,2-diphenyl-1-picrylhydrazyl-radical scavenging activities(23%), total antioxidant capacity (59%), total phenolic (23%), and flavonoid content (37%) with less number of days to 80% germination (57.1%), transpiration rate (53.9%), stomatal conductance (67.1%), non-photochemical quenching (82.8%), non-regulatory energy dissipation (24.3%), and H2O2 (25%) and O−2 levels (30%). Conclusion These findings elucidate the intricate mechanism behind the morphological, anatomical, physiological, fluorescent, and biochemical transformative effects of colchicine seed treatment on Acacia nilotica Linn. and offer valuable insights for quick production of A. nilotica’s plants with modification and enhancement from seeds through an eco-friendly approach.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2229Test

الوصول الحر: https://doaj.org/article/13d8096255fc4c3092d0ab4d7fdb3912Test

المؤلفون: Salma ‘Ariqotul Faridah ML, Ni Luh Arpiwi

المصدر: Simbiosis: Journal of Biological Sciences, Pp 73-82 (2024)

مصطلحات موضوعية: aspergillus, blotter test, fabaceae, germination percentage, viability, Biology (General), QH301-705.5

الوصف: The existence of malapari as a potential bioenergy plant relies on the natural populations in coastal forests, which are decreasing due to erosion and human activities. The provision of high-quality malapari seedlings, mostly through generative reproduction, is necessary to enhance its population in nature. However, one of the obstacles encountered during the seed germination process is the low germination capacity or ability of seeds due to poor seed quality. The presence of seed-borne pathogens affects seed health. This study aims to identify seed-borne pathogenic fungi and seed germination capacity in three malapari accessions. The research was carried out at the Plant Taxonomy (Mycology) Laboratory, Biology Program, Faculty of Mathematics and Natural Sciences, Udayana University, from January to February 2023, using malapari seeds obtained from three accessions, namely Tangguwisia, Umeanyar, and Seririt. The seeds were sterilized, followed by the blotter test method and direct sowing method on Potato Dextrose Agar media, then incubated at room temperature. Isolates that grew were reisolated on fresh PDA media to obtain pure cultures for subsequent observation and identification at the genus and/or species level. The results revealed the presence of Aspergillus flavus, Aspergillus niger, Rhizoctonia solani, Penicillium sp., Fusarium sp., Colletotrichum sp., and Alternaria sp. The percentage of germination capacity was highest in Seririt (38%), followed by Umeanyar (35%), and Tangguwisia (31%). Keywords: Aspergillus, blotter test, fabaceae, germination percentage, viability

وصف الملف: electronic resource

العلاقة: https://ejournal3.unud.ac.id/index.php/simbiosis/article/view/188Test; https://doaj.org/toc/2337-7224Test

الوصول الحر: https://doaj.org/article/7be097c5d0ae42f39d16da1cd986e7ecTest

المؤلفون: Reza Kamaei, Mohammad Kafi, Reza Tavakkol Afshari, Saeid Malekzadeh Shafaroudi, Jafar Nabati

المصدر: BMC Plant Biology, Vol 24, Iss 1, Pp 1-15 (2024)

مصطلحات موضوعية: Electrolyte leakage, Gene expression, Germination percentage, Protein concentration, Total sugar, Botany, QK1-989

الوصف: Abstract Background Onion seeds have limited storage capacity compared to other vegetable seeds. It is crucial to identify the mechanisms that induce tolerance to storage conditions and reduce seed deterioration. To address this goal, an experiment was conducted to evaluate changes in germination, biochemical, physiological, and molecular characteristics of onion seed landraces (Horand, Kazerun landraces and Zargan cultivar) at different aging levels (control, three-days and six-days accelerated aging, and natural aging for one year). Results The findings suggest that there was an increase in glucose, fructose, total sugar, and electrolyte leakage in the Horand (HOR), Kazerun (KAZ) landraces, and Zarghan (ZAR) cultivar, with Kazerun exhibiting the greatest increase. The percentage and rate of germination of Kazerun decreased by 54% and 33%, respectively, in six-day accelerated aging compared to the control, while it decreased by 12% and 14%, respectively, in Horand. Protein content decreased with increasing levels of aging, with a decrease of 26% in Kazerun landrace at six days of aging, while it was 16% in Horand landrace. The antioxidant activities of catalase, superoxide dismutase, and glutathione peroxidase decreased more intensively in Kazerun. The expression of AMY1, BMY1, CTR1, and NPR1 genes were lower in Kazerun landraces than in Horand and Zargan at different aging levels. Conclusions The AMY1, BMY1, CTR1, and NPR1 genes play a pivotal role in onion seed germination, and their downregulation under stressful conditions has been shown to decrease germination rates. In addition, the activity of CAT, SOD, and GPx enzymes decreased by seed aging, and the amount of glucose, fructose, total sugar and electrolyte leakage increased, which ultimately led to seed deterioration. Based on the results of this experiment, it is recommended to conduct further studies into the molecular aspects involved in onion seed deterioration. More research on the genes related to this process is suggested, as well as investigating the impact of different priming treatments on the genes expression involved in the onion seed aging process.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2229Test

الوصول الحر: https://doaj.org/article/7b8225f3f7e94cdda8ec40ae19cb5203Test

المؤلفون: Soujanya, Pingidi^{1, 1}, Sathappan, CT.¹, Dhanasekaran, D.¹

المصدر: Crop Research 59(1and2):47-51. 2024

المؤلفون: DHANYA V G, BANOTH VINESH, SRIPATHY K V, UDAYABHASKAR K, A N SINGH, SUNIL KANNAUJIA, SANJAY KUMAR, AMARESH, GOPI KISHAN, AMBU V

المصدر: The Indian Journal of Agricultural Sciences, Vol 94, Iss 4 (2024)

مصطلحات موضوعية: Barley, Ciz priming, Emergence potential, Germination percentage, Heat stress tolerance, High temperature stress, Agriculture

الوصف: Present study was carried during 2021–22 and 2022–23 at ICAR-Indian Institute Seed Science, Mau, Uttar Pradesh to evaluate the effect of thermopriming on inducing heat stress tolerance in barley (Hordeum vulgare L.) grown under suboptimal conditions. The experimental material consisted of two location specific varieties of barley, DWRB 101 and DWRB 123, with two distinct lots, one of fresh seeds and the other of seeds aged for 1-year duration. Temperature regime between 30 to 45°C with 5°C increment and 6, 12, 24, 36 and 48 h duration, was selected as the treatment combinations along with the control (non-treated seeds). Results showed that the quantum of temperature exposure as well as the duration has significant impact on the germination and seedling growth of barley under lab conditions. The ANOVA and Dunken multiple range test enumerated that, the seeds primed at 30°C for 12 h, has positively improved the germination potential and seedling vigour index when compared to the respective lower and higher durations of exposure and revealed it as the best treatment combination. The subsequent year of evaluation of emergence potential of the treated seeds under an artificially induced high temperature stress revealed that the selected treatment was a good performer in inducing heat stress tolerance in barley, and can be chosen as solution to the high temperature stress induced through increasing atmospheric temperature at different stages of crop growth.

وصف الملف: electronic resource

العلاقة: https://epubs.icar.org.in/index.php/IJAgS/article/view/142065Test; https://doaj.org/toc/0019-5022Test; https://doaj.org/toc/2394-3319Test

الوصول الحر: https://doaj.org/article/b4c5c6553a484d8a9dab7bdc286f783cTest

المؤلفون: Abdelghani Bouchyoua, Mohamed Kouighat, Azzedine Hafid, Laila Ouardi, Abdelmajid Khabbach, Khalil Hammani, Abdelghani Nabloussi

المصدر: Journal of Agriculture and Food Research, Vol 15, Iss , Pp 100928- (2024)

مصطلحات موضوعية: Germination percentage, osmotic potential, Rapeseed, Root traits, Water stress, Agriculture (General), S1-972, Nutrition. Foods and food supply, TX341-641

الوصف: Rapeseed (Brassica napus L.) is an important oilseed crop well adapted to Mediterranean area. However, increasing drought, due to climate change, impairs its growth and development, mainly when this stress occurs early during germination. This study carried out in vitro conditions aimed to investigate the effect of water deficit on seed germination and seedling growth traits in 12 genotypes to select the most drought-tolerant ones. Drought conditions were created using polyethylene glycol 6000 to induce three osmotic potential levels, namely −0.7 MPa for moderate stress, −0.9 MPa for intermediate stress, and −1.1 MPa for severe stress. Germination percentage (GP), germination rate (GR), mean germination time (MGT), root length (RL), shoot length (SL), root-to-shoot ratio (RSR), seedling vigor index (SVI), shoot elongation rate (SER), and root elongation rate (RER) were measured/calculated. ANOVA and PCA were performed to analyze the data gathered. Results showed a significant effect of genotype, drought, and drought × genotype interaction on all studied parameters. Under severe drought conditions, overall values of GP, GR, RL, SL, SER, RER, and SVI decreased by 87.15 %, 53.29 %, 9.33 %, 4.73 %, 78 %, 82 %, and 96 %, respectively, while those of MGT and RSR increased by 56 % and 76 %, respectively, when compared to unstressed conditions. However, the genotypes studied reacted differently to the three levels of water stress. Based on PCA results, RL, RER, and GP proved to be the most discriminating of drought-tolerant genotypes. Overall, higher drought tolerance was associated with higher values in RL, SL, and SVI. Accordingly, the variety ‘Nap10’ and, to a lesser extent, ‘Baraka’ and ‘Nap9’ exhibited higher drought tolerance than the rest of genotypes, which makes them potential cultivars for dry areas. However, further studies in real field conditions are needed to confirm their tolerance to drought occurring at germination as well as during other stages, mainly flowering.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2666154323004350Test; https://doaj.org/toc/2666-1543Test

الوصول الحر: https://doaj.org/article/0676cf8fe8224ab599b0cd3507b2f9f8Test

المؤلفون: Govind, Mukesh Kumar, Manish Kumar, Hardeep, Deepak Sangwan

المصدر: Environment Conservation Journal, Vol 25, Iss 1 (2024)

مصطلحات موضوعية: Azotobacter, Bael, Bioinoculants, Germination percentage, Gibberellic acid, Seed priming, Environmental sciences, GE1-350

الوصف: The current experiment was conducted during 2020-21 at the Microbiology Laboratory, CCS Haryana Agricultural University, Regional Research Station Bawal to examine the impact of bio-inoculants and chemicals seed priming on bael seed germination. There were 15 seed priming treatments i.e., control, IBA @ 100 ppm and 50 ppm for 24 hours, NAA @ 50 and 100 ppm for 24 hours, GA3 @ 50 and 100 ppm for 24 hours, KNO3 @ 1 per cent for 24 hours, Azotobacter (HT 54) for 30 minutes, Trichoderma viride for 30 minutes, Rhizobium (CK 16) for 30 minutes, PSB (P 36) for 30 minutes, hot water for 30 minutes, nitric acid for 3 minutes, sulphuric acid for 3 minutes. Among different seed priming treatments, shortest germination time (12.7 days) was recorded with sulphuric acid for 3 minutes in agar medium at 28 ⁰C under laboratory conditions and the highest germination percentage (83.3 %), dry weight per seedling (153.2 mg), seedling length (12.2 cm) and vigour index I (976) and II (12256) were observed when bael seeds primed with GA3 @ 100 ppm for 24 hours under laboratory conditions.

وصف الملف: electronic resource

العلاقة: https://journal.environcj.in/index.php/ecj/article/view/2439Test; https://doaj.org/toc/0972-3099Test; https://doaj.org/toc/2278-5124Test

الوصول الحر: https://doaj.org/article/cb82241a302c485ba83d852a7e188eedTest

المؤلفون: Huangpan He, Handong Gao, Xiaoming Xue, Jiahui Ren, Xueqi Chen, Ben Niu

مصطلحات موضوعية: Ecology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, slight increase followed, relative electrical conductivity, raffinose family oligosaccharides, raffinose also increased, div >< p, initial moisture content, trehalose also exhibited, phoebe chekiangensis <, germination percentage drops, correlation analysis revealed, significant negative correlation, seed viability caused, natural desiccation treatment, seed moisture content, moisture content, p <, germination percentage, chekiangensis <, seed viability, results revealed, natural desiccation, seed germination, significant relationship, significant decrease, seed desiccation, seed decreased

الوصف: One-way ANOVA analysis was used for the data. Different lowercase letters represent significant differences at the p < 0.05 level according to the Duncan test. Data represent mean ± SE. “-” means the data is not detected. (DOCX)

العلاقة: https://figshare.com/articles/journal_contribution/Changes_of_soluble_carbohydrates_in_seeds_of_i_Phoebe_chekiangensis_i_during_desiccation_/25364604Test

الإتاحة: https://doi.org/10.1371/journal.pone.0299669.s001Test

المؤلفون: Huangpan He, Handong Gao, Xiaoming Xue, Jiahui Ren, Xueqi Chen, Ben Niu

مصطلحات موضوعية: Ecology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, slight increase followed, relative electrical conductivity, raffinose family oligosaccharides, raffinose also increased, div >< p, initial moisture content, trehalose also exhibited, phoebe chekiangensis <, germination percentage drops, correlation analysis revealed, significant negative correlation, seed viability caused, natural desiccation treatment, seed moisture content, moisture content, p <, germination percentage, chekiangensis <, seed viability, results revealed, natural desiccation, seed germination, significant relationship, significant decrease, seed desiccation, seed decreased

الوصف: The regression line is represented by the black line. GP: Germination percentage, SS: Soluble sugar. (DOCX)

العلاقة: https://figshare.com/articles/journal_contribution/Linear_regression_analysis_between_germination_percentage_and_some_sugar_compounds_/25364607Test

الإتاحة: https://doi.org/10.1371/journal.pone.0299669.s002Test

المؤلفون: Cué-García, Jorge Luis, Ramírez-López, Jorge-Luis, Chagna Ávila, Eduardo Jaime

المصدر: Ciência Florestal; Vol. 34 No. 1 (2024): Publicação Contínua; e83757 ; Ciência Florestal; v. 34 n. 1 (2024): Publicação Contínua; e83757 ; 1980-5098 ; 0103-9954

مصطلحات موضوعية: Scarification, Walnut, Dormancy, Germination percentage, Germination vigor, Escarificación, Nuez, Latencia, Porcentaje de germinación, Vigor de germinación

الوصف: The objective was to determine the treatments and substrates that show the best behavior in relation to the germination of Juglans neotropica seeds. Five physical treatments were used in experiment one and in experiment two three physical treatments and three substrates were combined with completely randomized designs. Germination was monitored daily from 30 days after sowing. The evaluation was related to the germination capacity and the distribution of germination over time, such as: germination percentage, germination vigor, average germination time, speed coefficient, germination speed index and germination uniformity coefficient. The pre-germination treatments with a temperature of 40 and 50 oC in a stove with forced ventilation for two hours and the control, showed significant differences for all the variables, except the coefficient of uniformity, with respect to the scarification treatments with sanding. The highest accumulated germination percentage, 76%, was reached with pre-germination treatments at a temperature of 40 oC in an oven with forced ventilation for two hours. The interaction of substrates with pre-germination treatments do not show significant differences in the germination variables analyzed. Juglans neotropica seeds show asynchronous germination. ; Se indagó determinar los tratamientos y sustratos más efectivos para la germinación de semillas de Juglans neotropica. Se realizaron dos experimentos: en el primero se utilizaron cinco tratamientos físicos, mientras que en el segundo se combinaron tres tratamientos físicos y tres sustratos, en un diseño completamente al azar. Se monitoreó la germinación de forma diaria, a partir de los 30 días después de la siembra, y se evaluó la capacidad de germinación y la distribución de la germinación en el tiempo, considerando variables como: el porcentaje y vigor de germinación, tiempo promedio de germinación, coeficiente e índice de velocidad de germinación y coeficiente de uniformidad de germinación. Se encontró que los tratamientos de pregerminación ...

وصف الملف: application/pdf

العلاقة: https://periodicos.ufsm.br/cienciaflorestal/article/view/83757/63459Test; https://periodicos.ufsm.br/cienciaflorestal/article/view/83757Test

الإتاحة: https://doi.org/10.5902/1980509883757Test
https://periodicos.ufsm.br/cienciaflorestal/article/view/83757Test