المؤلفون: Biagioni Emanuela, Ferrari Elena, Gatto Ilenia, Serio Lucia, Farinelli Carlotta, Coloretti Irene, Talamonti Marta, Tosi Martina, Meschiari Marianna, Tonelli Roberto, Venturelli Claudia, Mussini Cristina, Clini Enrico, Sarti Mario, Cossarizza Andrea, Busani Stefano, Girardis Massimo.

المساهمون: Biagioni, Emanuela, Ferrari, Elena, Gatto, Ilenia, Serio, Lucia, Farinelli, Carlotta, Coloretti, Irene, Talamonti, Marta, Tosi, Martina, Meschiari, Marianna, Tonelli, Roberto, Venturelli, Claudia, Mussini, Cristina, Clini, Enrico, Sarti, Mario, Cossarizza, Andrea, Busani, Stefano, Girardis, Massimo.

مصطلحات موضوعية: COVID19, Acute Respiratory Distress Syndrome, Intensive Care Unit, Mechanical Ventilation, treatment

الوصف: The aim of our study was to evaluate whether the introduction of SDD in a structured protocol for VAP prevention was effective in reducing the occurrence of ventilator associated pneumonia (VAP) in COVID19 patients without changes in the microbiological pattern of antibiotic resistances. This observational pre-post study including adult patients requiring invasive mechanical ventilation (IMV) for severe respiratory failure related to SARS-CoV-2 admitted in three COVID19 intensive care units (ICUs) in an Italian hospital from February 22, 2020, to March 8, 2022. Selective digestive decontamination (SDD) was introduced from the end of April 2021 in the structured protocol for VAP prevention. The SDD consisted of a tobramycin sulphate, colistin sulphate and amphotericin B suspension applied in the patient oropharynx and the stomach via nasogastric tube. Three-hundred forty-eight patients were included in the study. In the 86 patients (32,9%) who received SDD the occurrence of VAP decreased by 7,7% (p = 0,192) compared to patients who did not receive SDD. Onset time of VAP, the occurrence of multidrug-resistant microorganisms AP, the length of invasive mechanical ventilation and hospital mortality were similar in patients who received and who did not receive SDD. The multivariate analysis adjusted for confounders showed that the use of SDD reduces the occurrence of VAP (HR 0,536, CI 0,338-0,851; p = 0,017) Our pre-post observational study indicates that the use of SDD in a structured protocol for VAP prevention seems to reduce the occurrence of VAP without changes in the incidence of multidrug-resistant bacteria in COVID19 patients.

العلاقة: info:eu-repo/semantics/altIdentifier/wos/WOS:000939097800001; volume:12; issue:4; firstpage:1; lastpage:9; journal:JOURNAL OF CLINICAL MEDICINE; https://hdl.handle.net/11380/1296908Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85149113342

الإتاحة: https://doi.org/10.3390/jcm12041432Test
https://hdl.handle.net/11380/1296908Test

المؤلفون: Capriello, Silvia, Ferrari, Silvia Martina, Gatto, Ilenia, Santaguida, Maria Giulia, Fallahi, Poupak, Antonelli, Alessandro, Mangino, Giorgio, Romeo, Giovanna, Virili, Camilla, Centanni, Marco

المساهمون: Capriello, Silvia, Ferrari, Silvia Martina, Gatto, Ilenia, Santaguida, Maria Giulia, Fallahi, Poupak, Antonelli, Alessandro, Mangino, Giorgio, Romeo, Giovanna, Virili, Camilla, Centanni, Marco

مصطلحات موضوعية: B regulatory cell, Hashimoto’s thyroiditi, Th17, polyautoimmunity, systemic sclerosi, human, interleukin-10, autoimmune disease, B-Lymphocytes, regulatory, Hashimoto disease, scleroderma, systemic

الوصف: Systemic sclerosis (SSc) is a systemic autoimmune disease in which gastrointestinal disorders represent a complication in up to 90% of patients. SSc may associate with thyroid autoimmune disorders, with Hashimoto's thyroiditis (HT) being the more prevalent worldwide. Previous studies have examined the behavior of Th17 lymphocytes and Breg cells in patients with HT and concomitant autoimmune organ-specific disorders. These immune phenotypes seem to play a significant role in the pathogenesis of both these autoimmune processes, but their behavior when these two disorders coexist has not been described. We analyzed Th17 and Breg (CD24hiCD38hi) cell subsets in 50 subjects (45F/5M; median age = 49 years): 18 were healthy donors (HD), 20 had isolated HT, and 12 had SSc, seven of whom had both HT and SSc. Breg cells' function was also evaluated by measuring their IL-10 production when stimulated by specific activators. An increased percentage of Th17 lymphocytes characterized HT patients as compared to both HD and the whole group of SSc patients (p = 0.0018). On the contrary, the percentage of unstimulated Breg cells in SSc patients was higher (p = 0.0260), either associated or not with HT, as compared to both HT patients and HD, which, instead, showed a similar percentage of Breg cells. Following a specific stimulation with CpG, the percentages of Breg cells were increased in the whole sample of SSc patients (p < 0.001) as well as in isolated SSc and in SSc+HT ones as compared to isolated HT. However, qualitative analysis, obtained through the detection of the IL-10-producing phenotype, revealed that the percentage of CpG-stimulated CD24hiCD38hi-IL10+cells was significantly decreased in SSc patients (p < 0.0001) with no difference between isolated SSc and SSc+HT patients. The IL-10-producing phenotype was instead slightly increased in HT patients as compared to HD (4.1% vs. 2.8%). The presence of SSc seems to be characterized by an enrichment of total Breg cells but by a reduced Breg IL-10-producing phenotype, ...

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/35874691; info:eu-repo/semantics/altIdentifier/wos/WOS:000829250800001; volume:13; firstpage:1; lastpage:8; numberofpages:8; journal:FRONTIERS IN IMMUNOLOGY; http://hdl.handle.net/11573/1652724Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85134307246

الإتاحة: https://doi.org/10.3389/fimmu.2022.921260Test
http://hdl.handle.net/11573/1652724Test

المؤلفون: Gatto, Ilenia, Biagioni, Emanuela, Coloretti, Irene, Viappiani, Serena, Busani, Stefano, Girardis, Massimo

المصدر: Journal of Anesthesia, Analgesia and Critical Care ; volume 2, issue 1 ; ISSN 2731-3786

الوصف: The ratio between arterial blood partial pressure of oxygen and fraction of inspired oxygen (PaO 2 /FiO 2 ) was largely used for grading and managing the respiratory failure in non-mechanically ventilated COVID-19. In these patients, the assessment of the true FiO 2 in the inspired mixture may be difficult with consequent inaccuracies in PaO 2 /FiO 2 assessment. In 30 severe COVID-19 patients, we observed that PaO 2 /FiO 2 values measured immediately before and after the transition from high-flow nasal cannula (HFNC) to one commercially available Venturi mask O 2 therapy were similar (bias mean value 0, standard deviation 23 mmHg). In COVID-19 patients recovering from respiratory failure, PaO 2 /FiO 2 is not different whether measured with a commercially available Venturi mask or HFNC.

الإتاحة: https://doi.org/10.1186/s44158-022-00051-wTest

المؤلفون: Caciagli, Valeria, Coloretti, Irene, Talamonti, Marta, Farinelli, Carlotta, Gatto, Ilenia, Biagioni, Emanuela, Sarti, Mario, Franceschini, Erica, Meschiari, Marianna, Mussini, Cristina, Tonelli, Roberto, Clini, Enrico, Girardis, Massimo, Busani, Stefano

المصدر: Viruses (1999-4915); Nov2023, Vol. 15 Issue 11, p2260, 11p

مصطلحات موضوعية: PULMONARY aspergillosis, CYTOMEGALOVIRUS diseases, COVID-19, CRITICALLY ill, BACTERIAL diseases, PROPORTIONAL hazards models, CYTOMEGALOVIRUSES

مستخلص: COVID-19-associated invasive pulmonary aspergillosis (CAPA) is common and is associated with poor outcomes in critically ill patients. This prospective observational study aimed to explore the association between CAPA development and the incidence and prognosis of cytomegalovirus (CMV) reactivation in critically ill COVID-19 patients. We included all consecutive critically ill adult patients with confirmed COVID-19 infection who were admitted to three COVID-19 intensive care units (ICUs) in an Italian hospital from 25 February 2020 to 8 May 2022. A standardized procedure was employed for early detection of CAPA. Risk factors associated with CAPA and CMV reactivation and the association between CMV recurrence and mortality were estimated using adjusted Cox proportional hazard regression models. CAPA occurred in 96 patients (16.6%) of the 579 patients analyzed. Among the CAPA population, 40 (41.7%) patients developed CMV blood reactivation with a median time of 18 days (IQR 7–27). The CAPA+CMV group did not exhibit a significantly higher 90-day mortality rate (62.5% vs. 48.2%) than the CAPA alone group (p = 0.166). The CAPA+CMV group had a longer ICU stay, fewer ventilation-free days, and a higher rate of secondary bacterial infections than the control group of CAPA alone. In the CAPA population, prior immunosuppression was the only independent risk factor for CMV reactivation (HR 2.33, 95% C.I. 1.21–4.48, p = 0.011). In critically ill COVID-19 patients, CMV reactivation is common in those with a previous CAPA diagnosis. Basal immunosuppression before COVID-19 appeared to be the primary independent variable affecting CMV reactivation in patients with CAPA. Furthermore, the association of CAPA+CMV versus CAPA alone appears to impact ICU length of stay and secondary bacterial infections but not mortality. [ABSTRACT FROM AUTHOR]

: Copyright of Viruses (1999-4915) is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Campani, Simone, Talamonti, Marta, Dall'Ara, Lorenzo, Coloretti, Irene, Gatto, Ilenia, Biagioni, Emanuela, Tosi, Martina, Meschiari, Marianna, Tonelli, Roberto, Clini, Enrico, Cossarizza, Andrea, Guaraldi, Giovanni, Mussini, Cristina, Sarti, Mario, Trenti, Tommaso, Girardis, Massimo

المصدر: Antibiotics (2079-6382); Oct2023, Vol. 12 Issue 10, p1536, 9p

مصطلحات موضوعية: BACTERIAL proteins, INTENSIVE care units, BACTERIAL diseases, C-reactive protein, ETIOLOGY of diseases

مستخلص: In COVID-19 patients, procalcitonin (PCT) and C-reactive protein (CRP) performance in identifying bacterial infections remains unclear. Our study aimed to evaluate the association of PCT and CRP with secondary infections acquired during ICU stay in critically ill COVID-19 patients. This observational study included adult patients admitted to three COVID-19 intensive care units (ICUs) from February 2020 to May 2022 with respiratory failure caused by SARS-CoV-2 infection and ICU stay ≥ 11 days. The values of PCT and CRP collected on the day of infection diagnosis were compared to those collected on day 11 after ICU admission, the median time for infection occurrence, in patients without secondary infection. The receiver operating characteristic curve (ROC) and multivariate logistic model were used to assess PCT and CRP association with secondary infections. Two hundred and seventy-nine patients were included, of whom 169 (60.6%) developed secondary infection after ICU admission. The PCT and CRP values observed on the day of the infection diagnosis were larger (p < 0.001) than those observed on day 11 after ICU admission in patients without secondary infections. The ROC analysis calculated an AUC of 0.744 (95%CI 0.685–0.803) and 0.754 (95%CI 0.695–0.812) for PCT and CRP, respectively. Multivariate logistic models showed that PCT ≥ 0.16 ng/mL and CRP ≥ 1.35 mg/dL were associated (p < 0.001) with infections acquired during ICU stay. Our results indicated that in COVID-19 patients, PCT and CRP values were associated with infections acquired during the ICU stay and can be used to support, together with clinical signs, rather than predict or rule out, the diagnosis of these infections. [ABSTRACT FROM AUTHOR]

: Copyright of Antibiotics (2079-6382) is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Santaguida, Maria Giulia, Gatto, Ilenia, Mangino, Giorgio, Virili, Camilla, Stramazzo, Ilaria, Fallahi, Poupak, Antonelli, Alessandro, Gargiulo, Patrizia, Romeo, Giovanna, Centanni, Marco

المساهمون: Santaguida, Maria Giulia, Gatto, Ilenia, Mangino, Giorgio, Virili, Camilla, Stramazzo, Ilaria, Fallahi, Poupak, Antonelli, Alessandro, Gargiulo, Patrizia, Romeo, Giovanna, Centanni, Marco

الوصف: Hashimoto's thyroiditis (HT) may occur associated with celiac disease (CD). Regulatory B cells (Breg) subsets have been shown to play a significant role in autoimmune processes. Therefore, we have characterized their distribution in the peripheral blood obtained from 10 patients with isolated HT, 10 patients with HT + CD, 9 patients with isolated CD, and 9 healthy donors (HD). Th17 cells were significantly increased in patients with HT and in patients bearing both HT and CD, while patients with isolated CD exhibited a lower percentage of Th17, as compared with healthy donors. CD24hiCD38hi Breg cells were significantly higher in patients with HT + CD and in patients with isolated CD as compared to both HD patients and patients with isolated HT (p = 0.0010). On the contrary, Breg memory phenotypes (CD24hiCD38- and CD24hiCD27+) significantly decreased in patients with HT + CD as compared with the isolated disorders. Following CpG oligodeoxynucleotide stimulation, IL-10+ CD24hiCD38hi Breg cells were similar in all groups of patients, despite these cells would have been higher in CD patients. In conclusion, celiac disease, isolated and even more when associated with HT, determines a peculiar behavior of Breg cells which are increased in number but possibly functionally defective. Furthermore, the association CD + HT was characterized by a reduction of Breg memory subsets as compared with the isolated disorders. The behavior of Th17 subset in patients with celiac disease associated with HT might have been sensitive to the effect of long-lasting GFD, and it is essentially determined by the presence of thyroid autoimmunity.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/30402096; info:eu-repo/semantics/altIdentifier/wos/WOS:000447904100001; volume:2018; firstpage:1; lastpage:6; numberofpages:6; journal:INTERNATIONAL JOURNAL OF ENDOCRINOLOGY; http://hdl.handle.net/11568/936592Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85060611407; https://www.hindawi.com/journals/ije/2018/5290865Test/

الإتاحة: https://doi.org/10.1155/2018/5290865Test
http://hdl.handle.net/11568/936592Test
https://www.hindawi.com/journals/ije/2018/5290865Test/

المؤلفون: Biagioni, Emanuela, Ferrari, Elena, Gatto, Ilenia, Serio, Lucia, Farinelli, Carlotta, Coloretti, Irene, Talamonti, Marta, Tosi, Martina, Meschiari, Marianna, Tonelli, Roberto, Venturelli, Claudia, Mussini, Cristina, Clini, Enrico, Sarti, Mario, Cossarizza, Andrea, Busani, Stefano, Girardis, Massimo.

مصطلحات موضوعية: treatment, COVID19, Mechanical Ventilation, Acute Respiratory Distress Syndrome, Intensive Care Unit

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=od______3674::38b5f957304b171ef19d37569fb702abTest
https://hdl.handle.net/11380/1296908Test

المؤلفون: Coloretti, Irene, Farinelli, Carlotta, Emanuela, Biagioni, Gatto, Ilenia, Munari, Elena, Dall'Ara, Lorenzo, Busani, Stefano, Meschiari, Marianna, Tonelli, Roberto, Mussini, Cristina, Guaraldi, Giovanni, Cossarizza, Andrea, Clini, Enrico, Girardis, Massimo

مصطلحات موضوعية: treatment, COVID19, Mechanical Ventilation, Acute Respiratory Distress Syndrome, Intensive Care Unit

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=od______3674::8825a0bafd1b364bb6729b2fa2227ad0Test
https://jtd.amegroups.com/article/view/75482/pdfTest

المؤلفون: SANTAGUIDA, MARIA GIULIA, GATTO, ILENIA, MANGINO, GIORGIO, VIRILI, CAMILLA, Stramazzo, Ilaria, Fallahi, Poupak, Antonelli, Alessandro, SEGNI, Maria, ROMEO, Giovanna, CENTANNI, Marco

المساهمون: Santaguida, MARIA GIULIA, Gatto, Ilenia, Mangino, Giorgio, Virili, Camilla, Stramazzo, Ilaria, Fallahi, Poupak, Antonelli, Alessandro, Segni, Maria, Romeo, Giovanna, Centanni, Marco

مصطلحات موضوعية: Breg cell, cellular immunity, hashimoto's thyroiditi, polyglandular autoimmune syndrome, thyroid autoimmunity

الوصف: Hashimoto thyroiditis (HT) may occur isolated or associated with other non-endocrine autoimmune disorders (NEAD). No data are available about Breg cells in these disorders and this represented the aim of the study. Th17 and Breg cells subset were characterized on peripheral blood mononuclear cells isolated from 18 healthy donors (HD), 19 patients with isolated HT and 26 patients with HT+NEAD. Th17 were higher in patients with isolated HT than in HD but no further changes were seen in patients with HT+NEAD. CD24(hi)CD38(hi) unstimulated Breg cells were similar in HT patients and in HD, but significantly higher in patients with HT+NEAD than in both HT and in HD. CD19(+)CD24(hi)CD27(+) Breg memory phenotype was similar in HD and in HT patients, but decreased in patients with HT+NEAD (23.4%vs38.5%). Upon CpG-stimulation, CD24(hi)CD38(hi) IL-10(+) Breg cells were higher in HT patients than in HD (3.9%vs1.8%) but similar in patients with HT+NEAD (2.4%).

وصف الملف: STAMPA

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/28461108; info:eu-repo/semantics/altIdentifier/wos/WOS:000414888300005; volume:184; firstpage:42; lastpage:47; numberofpages:6; journal:CLINICAL IMMUNOLOGY; http://hdl.handle.net/11573/953877Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85018985338

الإتاحة: https://doi.org/10.1016/j.clim.2017.04.012Test
http://hdl.handle.net/11573/953877Test

المؤلفون: SICILIANO, CAMILLA, BORDIN, ANTONELLA, IBRAHIM, MOHSEN, CHIMENTI, ISOTTA, CASSIANO, FRANCESCO, GATTO, ILENIA, MANGINO, GIORGIO, COCCIA, ANDREA, MIGLIETTA, SELENIA, BASTIANELLI, DANIELA, PETROZZA, Vincenzo, CALOGERO, ANTONELLA, FRATI, GIACOMO, DE FALCO, ELENA

المساهمون: Siciliano, Camilla, Bordin, Antonella, Ibrahim, Mohsen, Chimenti, Isotta, Cassiano, Francesco, Gatto, Ilenia, Mangino, Giorgio, Coccia, Andrea, Miglietta, Selenia, Bastianelli, Daniela, Petrozza, Vincenzo, Calogero, Antonella, Frati, Giacomo, DE FALCO, Elena

مصطلحات موضوعية: mesenchymal stem cell, adipose tissue, platelet lysate angiogenesi, transmission electron microscopy

الوصف: Indirect evidence suggests that adipose tissue-derived stromal cells (ASCs) possess different physiological and biological variations related to the anatomical localization of the adipose depots. Accordingly, to investigate the influence of the tissue origin on the intrinsic properties of ASCs and to assess their response to specific stimuli, we compared the biological, functional and ultrastructural properties of two ASC pools derived from mediastinal and subcutaneous depots (thoracic compartment) by means of supplements such as platelet lysate (PL) and FBS. Subcutaneous ASCs exhibited higher proliferative and clonogenic abilities than mediastinal counterpart, as well as increased secreted levels of IL-6 combined with lower amount of VEGF-C. In contrast, mediastinal ASCs displayed enhanced pro-angiogenic and adipogenic differentiation properties, increased cell diameter and early autophagic processes, highlighted by electron microscopy. Our results further support the hypothesis that the origin of adipose tissue significantly defines the biological properties of ASCs, and that a homogeneric function for all ASCs cannot be assumed.

وصف الملف: ELETTRONICO

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/27614132; info:eu-repo/semantics/altIdentifier/wos/WOS:000389523200020; volume:17; issue:2; firstpage:342; lastpage:351; numberofpages:10; journal:STEM CELL RESEARCH; http://hdl.handle.net/11573/878709Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84986000905; http://www.sciencedirect.com/science/article/pii/S1873506116300988Test

الإتاحة: https://doi.org/10.1016/j.scr.2016.07.010Test
http://hdl.handle.net/11573/878709Test
http://www.sciencedirect.com/science/article/pii/S1873506116300988Test