المؤلفون: Gaelle Cuzon, Rémy A Bonnin, Patrice Nordmann

المصدر: PLoS ONE, Vol 8, Iss 4, p e61322 (2013)

مصطلحات موضوعية: Medicine, Science

الوصف: BACKGROUND: The NDM-1 carbapenemase has been identified in 2008 in Enterobacteriaceae. Since then, several reports have emphasized its rapid dissemination throughout the world. The spread of NDM carbapenemases involve several bla NDM gene variants associated with various plasmids among several Gram negative species. METHODOLOGY: A multidrug-resistant E. coli isolate recovered from urine of a patient who had travelled to Burma has been characterized genetically and biochemically. PRINCIPAL FINDINGS: E. coli COU was resistant to all antibiotics tested except amikacin, tigecycline, fosfomycin, and chloramphenicol. Analysis of the antibiotic resistance traits identified a metallo-ß-lactamase, a novel NDM variant, NDM-7. It differs from NDM-4 by a single amino acid substitution sharing an identical extended spectrum profile towards carbapenems. The bla NDM-7 gene was located on an untypeable conjugative plasmid and associated with a close genetic background similar to those described among the bla NDM-1 genes. The isolate also harbours bla CTXM-15 and bla OXA-1 genes and belonged to ST167. SIGNIFICANCE: This study highlights that spread of NDM producers correspond to spread of multiple bla NDM genes and clones and therefore will be difficult to control.

وصف الملف: electronic resource

العلاقة: http://europepmc.org/articles/PMC3625146?pdf=renderTest; https://doaj.org/toc/1932-6203Test

الوصول الحر: https://doaj.org/article/a2a3003ba63c49c08370a19a2fa24b4dTest

المؤلفون: Jean-Philippe Lavigne, Gaelle Cuzon, Christophe Combescure, Gisèle Bourg, Albert Sotto, Patrice Nordmann

المصدر: PLoS ONE, Vol 8, Iss 7, p e67847 (2013)

مصطلحات موضوعية: Medicine, Science

الوصف: Klebsiella pneumoniae carbapenemase (KPC) is a carbapenemase increasingly reported worldwide in Enterobacteriaceae. The aim of this study was to analyze the virulence of several KPC-2-producing K. pneumoniae isolates. The studied strains were (i) five KPC-2 clinical strains from different geographical origins, belonging to different ST-types and possessing plasmids of different incompatibility groups; (ii) seven transformants obtained after electroporation of either these natural KPC plasmids or a recombinant plasmid harboring only the bla KPC-2 gene into reference strains K. pneumoniae ATCC10031/CIP53153; and (iii) five clinical strains cured of plasmids. The virulence of K. pneumoniae isolates was evaluated in the Caenorhabditis elegans model. The clinical KPC producers and transformants were significantly less virulent (LT50: 5.5 days) than K. pneumoniae reference strain (LT50: 4.3 days) (p

وصف الملف: electronic resource

العلاقة: http://europepmc.org/articles/PMC3699468?pdf=renderTest; https://doaj.org/toc/1932-6203Test

الوصول الحر: https://doaj.org/article/0a1f4a7a0dc842c7a25b62280e7c0f46Test

المؤلفون: Rishma Amarsy, David Trystram, Emmanuelle Cambau, Catherine Monteil, Sandra Fournier, Juliette Oliary, Helga Junot, Pierre Sabatier, Raphaël Porcher, Jérôme Robert, Vincent Jarlier, Guillaume Arlet, Laurence Armand Lefevre, Alexandra Aubry, Laurent Belec, Béatrice Bercot, Stéphane Bonacorsi, Vincent Calvez, Etienne Carbonnelle, Stéphane Chevaliez, Jean-Winoc Decousser, Constance Delaugerre, Diane Descamps, Florence Doucet-Populaire, Jean-Louis Gaillard, Antoine Garbarg- Chenon, Elyanne Gault, Jean-Louis Herrmann, Jérôme Le Goff, Jean-Christophe Lucet, Jean-Luc Mainardi, Anne-Geneviève Marcellin, Laurence Morand-Joubert, Xavier Nassif, Jean-Michel Pawlotsky, Anne-Marie Roque Afonso, Martin Rottman, Christine Rouzioux, Flore Rozenberg, François Simon, Nicolas Veziris, David Skurnik, Jean-Ralph Zahar, Guilene Barnaud, Typhaine Billard Pomares, Gaëlle Cuzon, Dominique Decré, Alexandra Doloy, Jean-Luc Donay, Laurence Drieux-Rouzet, Isabelle Durand, Agnès Ferroni, Vincent Fihman, Nicolas Fortineau, Camille Gomart, Nathalie Grall, Christelle Guillet Caruba, Françoise Jaureguy, Valérie Lalande, Luce Landraud, Véronique Leflon, Patricia Mariani, Liliana Mihaila, Didier Moissenet, Latifa Noussair, Isabelle Podglajen, Isabelle Poilane, Hélène Poupet, Emilie Rondinaud, Valérie Sivadon Tardy, Charlotte Verdet, Emmanuelle Vigier, Sophie Vimont Billarant

المصدر: International Journal of Infectious Diseases, Vol 114, Iss , Pp 90-96 (2022)

مصطلحات موضوعية: COVID-19, Blood culture, Bloodstream infection incidence, Antibiotic consumption, Antimicrobial resistance, Infectious and parasitic diseases, RC109-216

الوصف: Objectives: This study measured the impact of the first wave of COVID-19 pandemic (COVID-19) (March–April 2020) on the incidence of bloodstream infections (BSIs) at Assistance Publique – Hôpitaux de Paris (APHP), the largest multisite public healthcare institution in France. Methods: The number of patient admission blood cultures (BCs) collected, number of positive BCs, and antibiotic resistance and consumption were analysed retrospectively for the first quarter of 2020, and also for the first quarter of 2019 for comparison, in 25 APHP hospitals (ca. 14 000 beds). Results: Up to a fourth of patients admitted in March–April 2020 in these hospitals had COVID-19. The BSI rate per 100 admissions increased overall by 24% in March 2020 and 115% in April 2020, and separately for the major pathogens (Escherichia coli, Klebsiella pneumoniae, enterococci, Staphylococcus aureus, Pseudomonas aeruginosa, yeasts). A sharp increase in the rate of BSIs caused by microorganisms resistant to third-generation cephalosporins (3GC) was also observed in March–April 2020, particularly in K. pneumoniae, enterobacterial species naturally producing inducible AmpC (Enterobacter cloacae.), and P. aeruginosa. A concomitant increase in 3GC consumption occurred. Conclusions: The COVID-19 pandemic had a strong impact on hospital management and also unfavourable effects on severe infections, antimicrobial resistance, and laboratory work diagnostics.

العلاقة: http://www.sciencedirect.com/science/article/pii/S1201971221008213Test; https://doaj.org/toc/1201-9712Test; https://doaj.org/article/dbe9193e72ae446ebaf008bb25bb9ab4Test

الإتاحة: https://doi.org/10.1016/j.ijid.2021.10.034Test
https://doaj.org/article/dbe9193e72ae446ebaf008bb25bb9ab4Test

المؤلفون: Rémy A. Bonnin, Delphine Girlich, Agnès B. Jousset, Lauraine Gauthier, Gaëlle Cuzon, Pierre Bogaerts, Marisa Haenni, Jean-Yves Madec, Elodie Couvé-Deacon, Olivier Barraud, Nicolas Fortineau, Philippe Glaser, Youri Glupczynski, Laurent Dortet, Thierry Naas

المصدر: Scientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)

مصطلحات موضوعية: Medicine, Science

الوصف: In Enterobacterales, the most common carbapenemases are Ambler’s class A (KPC-like), class B (NDM-, VIM- or IMP-like) or class D (OXA-48-like) enzymes. This study describes the characterization of twenty-four OXA-23 or OXA-58 producing-Proteus mirabilis isolates recovered from human and veterinary samples from France and Belgium. Twenty-two P. mirabilis isolates producing either OXA-23 (n = 21) or OXA-58 (n = 1), collected between 2013 and 2018, as well as 2 reference strains isolated in 1996 and 2015 were fully sequenced. Phylogenetic analysis revealed that 22 of the 24 isolates, including the isolate from 1996, belonged to a single lineage that has disseminated in humans and animals over a long period of time. The bla OXA-23 gene was located on the chromosome and was part of a composite transposon, Tn6703, bracketed by two copies of IS15∆II. Sequencing using Pacbio long read technology of OXA-23-producing P. mirabilis VAC allowed the assembly of a 55.5-kb structure encompassing the bla OXA-23 gene in that isolate. By contrast to the bla OXA-23 genes, the bla OXA-58 gene of P. mirabilis CNR20130297 was identified on a 6-kb plasmid. The acquisition of the bla OXA-58 gene on this plasmid involved XerC-XerD recombinases. Our results suggest that a major clone of OXA-23-producing P. mirabilis is circulating in France and Belgium since 1996.

العلاقة: https://doi.org/10.1038/s41598-020-66161-zTest; https://doaj.org/toc/2045-2322Test; https://doaj.org/article/a61067770b2c4ff9b0017099f6999791Test

الإتاحة: https://doi.org/10.1038/s41598-020-66161-zTest
https://doaj.org/article/a61067770b2c4ff9b0017099f6999791Test

المؤلفون: Philippe Bidet, Anne Vachee, Aurélie Cointe, André Birgy, Emmanuel Cixous, Camille Jung, Philippe Traore, Sarah Ducrocq, Catherine Branger, Marion Decobert, Jocelyne Caillon, Benoit Starck, Said Aberrane, Sandra Timsit, Franck Labbee, Agnès Ferroni, Isabelle Andriantahina, M.-A. Dommergues, Isabelle Breant, Valérie Soussan-Banini, Marleèe Amara, François Dubos, Corinne Levy, Abdelmalek Belgaid, Hélène Garrec, Elsa Sobral, Rodrigue Dessein, Florence Doucet-Populaire, Vincent Gajdos, Irina Craiu, Valérie Sivadon-Tardy, Robert M. Cohen, Jack Breuil, Fouad Madhi, Olivier Vignaud, Stéphane Béchet, Hoang Vu-Thien, Marie Desmarest, H. Haas, Elise Launay, Sandra Biscardi, Emmanuel Grimprel, Didier Pinquier, Claire Amaris Hobson, Emilie Georget, Stéphane Bonacorsi, Anne Farges-Berth, Alain Fiacre, Aurélia Pitsch, Sophie Boyer, Bogdan Cojocaru, Laure Hees, Isabelle Poilane, Gaelle Cuzon, Laetitia Beraud, Marie-Noëlle Adam, Cécile Farrugia, Aurélien Galerne

المصدر: The Journal of antimicrobial chemotherapy. 76(11)

مصطلحات موضوعية: Microbiology (medical), medicine.medical_specialty, Urinary system, Urine, Cefpodoxime, Gastroenterology, Pharmacokinetics, Cefixime, Internal medicine, medicine, Humans, Pharmacology (medical), Mecillinam, Child, Clavulanic Acid, Pharmacology, business.industry, Ceftizoxime, Amdinocillin, Amoxicillin, Anti-Bacterial Agents, Infectious Diseases, Urinary Tract Infections, Amoxicillin-Potassium Clavulanate Combination, business, medicine.drug

الوصف: Objectives Oral treatment of febrile urinary tract infections (FUTIs) can be impaired by MDR Enterobacterales often combining ESBL and inhibitor-resistant genes. We studied the impact of β-lactamases and Enterobacterales’ genotypes on the cefixime, cefpodoxime and mecillinam ± amoxicillin/clavulanate MICs. Materials and methods In this multicentric study, we included 251 previously whole-genome-sequenced ESBL-producing Enterobacterales, isolated in French children with FUTIs. The MICs of cefixime, cefpodoxime, mecillinam alone and combined with amoxicillin/clavulanate were determined and analysed with respect to genomic data. We focused especially on the isolates’ ST and their type of β-lactamases. Clinical outcomes of patients who received cefixime + amoxicillin/clavulanate were also analysed. Results All isolates were cefixime and cefpodoxime resistant. Disparities depending on blaCTX-M variants were observed for cefixime. The addition of amoxicillin/clavulanate restored susceptibility for cefixime and cefpodoxime in 97.2% (MIC50/90 of 0.38/0.75 mg/L) and 55.4% (MIC50/90 of 1/2 mg/L) of isolates, respectively, whatever the ST, the blaCTX-M variants or the association with inhibitor-resistant β-lactamases (34.2%). All isolates were susceptible to mecillinam + amoxicillin/clavulanate with MIC50/90 of 0.19/0.25 mg/L, respectively. Neither therapeutic failure nor any subsequent positive control urine culture were reported for patients who received cefixime + amoxicillin/clavulanate as an oral relay therapy (n = 54). Conclusions Despite the frequent association of ESBL genes with inhibitor-resistant β-lactamases, the cefixime + amoxicillin/clavulanate MICs remain low. The in vivo efficacy of this combination was satisfying even when first-line treatment was ineffective. Considering the MIC distributions and pharmacokinetic parameters, mecillinam + amoxicillin/clavulanate should also be an alternative to consider when treating FUTIs in children.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd69c25b82377647a488e041974b4009Test
https://pubmed.ncbi.nlm.nih.gov/34453533Test

المؤلفون: Seher Yilmaz, Gaelle Cuzon, Laurent Dortet, Cécile Emeraud, Nicolas Fortineau

المصدر: Journal of Medical Microbiology

مصطلحات موضوعية: Microbiology (medical), medicine.medical_specialty, Time Factors, Sample (material), Blood volume, Microbiology, BacT/AlertVirtuo, accreditation, Specimen Handling, Bloodstream infection, Sepsis, medicine, Humans, Blood culture, Quality Indicators, Health Care, medicine.diagnostic_test, Bacteria, business.industry, Bact alert, General Medicine, quality indicators, Contamination rate, Clinical microbiology, Clinical Microbiology, Blood Culture, Emergency medicine, France, business

الوصف: Introduction. Blood culture (BC) remains the gold standard for the diagnosis of bloodstream infection. Clinical microbiology laboratories must ensure the quality of their BC process from receipt to definitive results. Aim. In this study, we followed the evolution of different quality indicators for BCs over the first year of implementation of the BacT/Alert Virtuo system in a French hospital. Methodology. In our laboratory, we instituted regular monitoring of several quality indicators to track (i) delays in sample registration, (ii) delays in loading BC bottles in our incubating system (BacT/Alert Virtuo) after registration, (iii) the volume of blood in bottles and (iv) the contamination rates. Results. For 53 892 BC bottles loaded in the BacT/Alert Virtuo from 23 January to 31 December 2019, the delays in sample registration, loading and unloading were respectively 3.5 h±0.016, 44 min±0.209 and 5.8 h±0.0727. Intriguingly, the automated process performed by the BacT/Alert Virtuo system to check the blood volume in bottles was only performed for 60 % of the loaded bottles. Among these, 30 % contained the recommended volume of blood (between 7 and 13 ml). Finally, the contamination rate was found to be 27.2 % for samples at our institution. Conclusions. The delays in sample registration, loading and unloading were found to be acceptable, even though they could be improved by ensuring a continuous service during the night duty period. Furthermore, the percentage of volumes measured is insufficient and must be improved and the majority of bottles do not contain the recommended blood volume.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a173fe999661dc2050dea234e93dd315Test
http://europepmc.org/articles/PMC8346724Test

المؤلفون: Rémy A. Bonnin, Gaëlle Cuzon, Laurent Poirel, Patrice Nordmann

المصدر: Emerging Infectious Diseases, Vol 19, Iss 5, Pp 822-823 (2013)

مصطلحات موضوعية: Acinetobacter baumannii, carbapenemase, NDM-1, ST85, bacteria, France, Medicine, Infectious and parasitic diseases, RC109-216

العلاقة: https://wwwnc.cdc.gov/eid/article/19/5/12-1618_articleTest; https://doaj.org/toc/1080-6040Test; https://doaj.org/toc/1080-6059Test; https://doaj.org/article/f330330af1a849b084c52ab9de4254caTest

الإتاحة: https://doi.org/10.3201/eid1905.121618Test
https://doaj.org/article/f330330af1a849b084c52ab9de4254caTest

المؤلفون: Pierre Bogaerts, Marisa Haenni, Elodie Couvé-Deacon, Rémy A. Bonnin, Jean-Yves Madec, Olivier Barraud, Lauraine Gauthier, Nicolas Fortineau, Philippe Glaser, Gaelle Cuzon, Laurent Dortet, Agnès B Jousset, Delphine Girlich, Thierry Naas, Youri Glupczynski

المساهمون: Team Resist [Le Kremlin-Bicêtre], Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes (IMVA-HB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, French National Reference Center for Antibiotic Resistance: Carbapenemase producing Enterobacteriaceae [Le Kremlin-Bicêtre], Ecologie et Evolution de la Résistance aux Antibiotiques / Ecology and Evolution of Antibiotics Resistance (EERA), Institut Pasteur [Paris]-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Sud Orsay-Centre National de la Recherche Scientifique (CNRS), Bacteriology-Hygiene unit [Le Kremlin-Bicêtre], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), CHU UCL Namur, Unité Antibiorésistance et Virulence Bactériennes, Laboratoire de Lyon [ANSES], Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques (RESINFIT), CHU Limoges-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), This work was partially funded by the University Paris-Sud, France. LD, TN and RAB are members of the Laboratory of Excellence in Research on Medication and Innovative Therapeutics (LERMIT) supported by a grant from the French National Research Agency (ANR-10-LABX-33) and by the Joint Programming Initiative on Antimicrobial Resistance (JPIAMR) DesInMBL [ANR-14-JAMR-002]., We want to thanks Pasteur International Bioressources Networking (PibNet, Paris, France) for providing whole genome sequencing facilities. We would like to thank the Transposon Registry for transposon nomenclature (https://transposon.lstmed.ac.uk/tn-registryTest). We thank INTEGRALL database for integron and gene cassette nomenclatures., ANR-10-LABX-0033,LERMIT,Research Laboratory on Drugs and Therapeutic Innovation(2010), ANR-14-JAMR-0002,DesInMBL,Structure-guided design of pan inhibitors of metallo-ß-lactamases(2014), Université Paris-Sud - Paris 11 (UP11)-Institut Pasteur [Paris] (IP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre National de la Recherche Scientifique (CNRS), Unité Antibiorésistance et Virulence Bactériennes (AVB), Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), UCL - (MGD) Laboratoire de biologie clinique, UCL - SSS/IREC/MONT - Pôle Mont Godinne, Institut Pasteur [Paris] (IP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Sud Orsay-Centre National de la Recherche Scientifique (CNRS), Bodescot, Myriam, Research Laboratory on Drugs and Therapeutic Innovation - - LERMIT2010 - ANR-10-LABX-0033 - LABX - VALID, programmation conjointe européenne sur la résistance antimicrobienne - Structure-guided design of pan inhibitors of metallo-ß-lactamases - - DesInMBL2014 - ANR-14-JAMR-0002 - JPI AMR - VALID

المصدر: Scientific Reports
Scientific Reports, Nature Publishing Group, 2020, 10 (1), pp.9160. ⟨10.1038/s41598-020-66161-z⟩
Scientific Reports, 2020, 10 (1), pp.9160. ⟨10.1038/s41598-020-66161-z⟩
Scientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
Scientific reports, Vol. 10, no. 1, p. 9160 [1-9] (2020)

مصطلحات موضوعية: DNA, Bacterial, 0301 basic medicine, clone (Java method), Lineage (genetic), Science, 030106 microbiology, Antimicrobial resistance, beta-Lactamases, Article, 03 medical and health sciences, Plasmid, Bacterial Proteins, Belgium, Recombinase, polycyclic compounds, Animals, Humans, Clinical microbiology, Proteus mirabilis, Gene, Genetics, Multidisciplinary, biology, Phylogenetic tree, integumentary system, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Chromosomes, Bacterial, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, 030104 developmental biology, Composite transposon, Genes, Bacterial, DNA Transposable Elements, bacteria, Medicine, France, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Plasmids

الوصف: In Enterobacterales, the most common carbapenemases are Ambler’s class A (KPC-like), class B (NDM-, VIM- or IMP-like) or class D (OXA-48-like) enzymes. This study describes the characterization of twenty-four OXA-23 or OXA-58 producing-Proteus mirabilis isolates recovered from human and veterinary samples from France and Belgium. Twenty-two P. mirabilis isolates producing either OXA-23 (n = 21) or OXA-58 (n = 1), collected between 2013 and 2018, as well as 2 reference strains isolated in 1996 and 2015 were fully sequenced. Phylogenetic analysis revealed that 22 of the 24 isolates, including the isolate from 1996, belonged to a single lineage that has disseminated in humans and animals over a long period of time. The blaOXA-23 gene was located on the chromosome and was part of a composite transposon, Tn6703, bracketed by two copies of IS15∆II. Sequencing using Pacbio long read technology of OXA-23-producing P. mirabilis VAC allowed the assembly of a 55.5-kb structure encompassing the blaOXA-23 gene in that isolate. By contrast to the blaOXA-23 genes, the blaOXA-58 gene of P. mirabilis CNR20130297 was identified on a 6-kb plasmid. The acquisition of the blaOXA-58 gene on this plasmid involved XerC-XerD recombinases. Our results suggest that a major clone of OXA-23-producing P. mirabilis is circulating in France and Belgium since 1996.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d440f191465409b9394bb12862e729cTest
https://www.hal.inserm.fr/inserm-02889214/documentTest

المؤلفون: Christian Dohna Schwake, Mohamed-Rida Benissa, Jordi Miatello, Pierre Tissieres, Philippe Durand, Cécile Dubois, Tamazoust Guiddir, Zied Merchaoui, Gaelle Cuzon

المساهمون: Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Endotoxines, Structures et Réponses de l'hôte (ESHR), Département Microbiologie (Dpt Microbio), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)

المصدر: Transplant Infectious Disease
Transplant Infectious Disease, 2019, ⟨10.1111/tid.13208⟩
Transplant Infectious Disease, Wiley, 2019, ⟨10.1111/tid.13208⟩

مصطلحات موضوعية: Male, medicine.medical_specialty, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Medizin, 030230 surgery, Liver transplantation, Single Center, Sepsis, sepsis, 03 medical and health sciences, 0302 clinical medicine, children, Risk Factors, Internal medicine, medicine, Humans, Risk factor, Child, Cause of death, Transplantation, liver transplantation, business.industry, Septic shock, Bacterial pneumonia, Bacterial Infections, Pneumonia, medicine.disease, 3. Good health, Infectious Diseases, multiresistant bacteria, Child, Preschool, nosocomial infection, Epidemiological Monitoring, Multivariate Analysis, Urinary Tract Infections, Female, 030211 gastroenterology & hepatology, business, Immunosuppressive Agents

الوصف: BACKGROUND: Infectious complications after pediatric liver transplantation frequently occur and are potentially serious. Data concerning strictly defined bacterial infections and their associated risk factors are lacking. METHODS: For the pediatric liver transplant postoperative period we analyzed data from the nosocomial infection surveillance (2006-2015). RESULTS: A total of 235 bacterial infections in 162 transplantations (47%) occurred, including 32 bacterial pneumonia cases, 104 surgical site infections, 27 urinary tract infections and 40 bloodstream infections. Sepsis was diagnosed in 127 cases (54%), severe sepsis in 22 (9%) cases and septic shock in 41 (17%) cases. Thirty patients (9%) died, and septic shock was the leading cause of death. The carrier status of multi-drug resistant bacteria and a tacrolimus level \textgreater20 ng/ml were independent risk factors for surgical site infections and the occurrence of severe sepsis or septic shock. The length of mechanical ventilation was an independent risk factor for pneumonia and surgical site infection. CONCLUSION: Bacterial infections in the early postoperative period after pediatric liver transplantation are associated with high morbidity and mortality. Physicians involved in the medical care of these patients should be aware of the specific risk factors, and further development of prevention programs is highly recommended.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::383ed8bd6c9539c7e178f3ebab520ec8Test
https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&origin=inward&scp=85076362027Test

المؤلفون: Eric Farfour, Rémy A. Bonnin, Isabelle Rosinski-Chupin, Philippe Glaser, Agnès B Jousset, Laurent Dortet, Delphine Girlich, Hélène Frech, Thierry Naas, Gaelle Cuzon, Nicolas Cabanel

المساهمون: Centre National de Référence Associé de la Résistance aux Antibiotiques, AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), Hôpital Bicêtre, Hôpital Bicêtre-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris-Sud - Paris 11 (UP11), Ecologie et Evolution de la Résistance aux Antibiotiques / Ecology and Evolution of Antibiotics Resistance (EERA), Institut Pasteur [Paris]-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Sud Orsay-Centre National de la Recherche Scientifique (CNRS), Structure, Dynamique, Fonction Et Expression Des Beta-Lactamases À Large Spectre, Université Paris-Sud - Paris 11 - Faculté de médecine (UP11 UFR Médecine), Université Paris-Sud - Paris 11 (UP11)-Université Paris-Sud - Paris 11 (UP11)-Centre National de Référence de la Résistance aux Antibiotiques (CNR), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Université de Bordeaux (UB), CHI Poissy-Saint-Germain, Hôpital Foch [Suresnes], This work was supported by the Assistance Publique–Hôpitaux de Paris, Université Paris Sud (grant number EA 7361), LabEx Laboratoire d’Excellence en Recherche sur le Médicament et l’InnovationThérapeutique, supported by the French National Research Agency (grant number Agence Nationale de la Recherche [ANR]-10-LABX-33), by a project of ANR LabEx Integrative Biology of Emerging Infectious Diseases, and the Joint Program Initiative on Antimicrobial Resistance (grant number ANR-14-JAMR-0002)., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-14-JAMR-0002,DesInMBL,Structure-guided design of pan inhibitors of metallo-ß-lactamases(2014), Centre National de Référence Associé de la Résistance aux Antibiotiques [Hôpital Bicêtre AP-HP] (CNRARA/Service de Microbiologie), Université Paris-Sud - Paris 11 (UP11)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Bicêtre, Université Paris-Sud - Paris 11 (UP11)-Institut Pasteur [Paris] (IP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre National de la Recherche Scientifique (CNRS)

المصدر: Clinical Infectious Diseases
Clinical Infectious Diseases, Oxford University Press (OUP), 2018, 67 (9), pp.1388-1394. ⟨10.1093/cid/ciy293⟩
Clinical Infectious Diseases, 2018, 67 (9), pp.1388-1394. ⟨10.1093/cid/ciy293⟩

مصطلحات موضوعية: Male, 0301 basic medicine, MESH: Fatal Outcome, Klebsiella pneumoniae, MESH: Klebsiella pneumoniae, Respiratory chain, MESH: beta-Lactamases, Bacteremia, Drug resistance, phylogeny, Fatal Outcome, carrier, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, polycyclic compounds, Medicine, MESH: Bacteremia, MESH: Bacterial Proteins, MESH: Microbial Sensitivity Tests, biology, MESH: Polymorphism, Single Nucleotide, within-host evolution, Anti-Bacterial Agents, 3. Good health, MESH: Klebsiella Infections, Infectious Diseases, NGS, Carrier State, MESH: Equipment Contamination, MESH: Carrier State, MESH: Whole Genome Sequencing, medicine.drug, Microbiology (medical), MESH: Mutation, 030106 microbiology, Virulence, Microbial Sensitivity Tests, MESH: Biofilms, Polymorphism, Single Nucleotide, beta-Lactamases, MESH: Endoscopy, MESH: Fimbriae, Bacterial, Microbiology, Evolution, Molecular, 03 medical and health sciences, Antibiotic resistance, Bacterial Proteins, MESH: Anti-Bacterial Agents, Drug Resistance, Bacterial, MESH: Drug Resistance, Bacterial, Humans, MESH: Humans, Whole Genome Sequencing, Colistin, business.industry, Endoscopy, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, MESH: Colistin, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, biology.organism_classification, medicine.disease, MESH: Male, Klebsiella Infections, KPC, Carriage, Biofilms, Fimbriae, Bacterial, Mutation, Equipment Contamination, business

الوصف: International audience; Background. Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-Kp) has emerged globally over the last decade as a major nosocomial pathogen that threatens patient care. These highly resistant bacteria are mostly associated with a single Kp clonal group, CG258, but the reasons for its host and hospital adaptation remain largely unknown. Methods. We analyzed the in vivo evolution of a colistin-resistant KPC-Kp CG258 strain that contaminated a patient following an endoscopy and was responsible for a fatal bacteremia 4.5 years later. Whole-genome sequencing was performed on 17 KPC-Kp isolates from this patient; single-nucleotide polymorphisms were analyzed and their implication in antimicrobial resistance and bacterial host adaptation investigated. Results. The patient KPC-Kp strain diversified over 4.5 years at a rate of 7.5 substitutions per genome per year, resulting in broad phenotypic modifications. After 2 years of carriage, all isolates restored susceptibility to colistin. Higher expression of the fimbriae conferred the ability to produce more biofilm, and the isolate responsible for a bacteremia grew in human serum. The convergent mutations occurring in specific pathways, such as the respiratory chain and the cell envelope, revealed a complex long-term adaptation of KPC-Kp. Conclusions. Broad genomic and phenotypic diversification and the parallel selection of pathoadaptive mutations might contribute to long-term carriage and virulence of KPC-Kp CG258 strains and to the dissemination of this clone.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60134fa1435e7d2c8e925dfa54da9578Test
https://doi.org/10.1093/cid/ciy293Test