المؤلفون: KUANG Xiaozhou, XIAO Wenjia, PAN Hao, CHEN Min, TENG Zheng

المصدر: Shanghai yufang yixue, Vol 36, Iss 2, Pp 143-149 (2024)

مصطلحات موضوعية: pediatric inpatient, group a rotavirus, g8p［8］ genotype, norovirus gⅱ, Medicine

الوصف: ObjectiveTo investigate the changes in the pathogen spectrum of viral diarrhea in local pediatric inpatients as well as any variations in genotypes of major pathogens during the COVID-19 control period.MethodsFecal samples were collected from the children

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1004-9231Test

الوصول الحر: https://doaj.org/article/4113d148e32f4a38949225047271f65dTest

المؤلفون: Osazee Izevbuwa

المصدر: Microbes and Infectious Diseases, Vol 4, Iss 3, Pp 777-791 (2023)

مصطلحات موضوعية: group a rotavirus, reverse transcription polymerase chain reaction, type vp7-g, type vp4-p, diarrhea, Infectious and parasitic diseases, RC109-216

الوصف: rotaviruses are one of the vital causative agents of acute gastroenteritis (AGE) in young children worldwide. Genotyping of detected RVA strains is needed for a more extensive knowledge of the epidemiology of rotaviral infections. This descriptive cross-sectional study aimed to evaluate the circulation of RVA genotypes in diarrheic children living in Edo State, Nigeria. A total of 400 stool samples collected from children less than five years with acute diarrhea were initially screened for RVA antigen by immumochromatographic method, and the RVA antigen-positive samples were subsequently analyzed using Reverse Transcription Polymerase Chain Reaction (RT-PCR), multiplex PCR and sequencing of the VP7 and VP4 gene segments of the RVA strains. Phylogenetic trees were constructed from the nucleotide sequences using the neighbor joining algorithm in MEGA software, version 6. Seventeen stool samples were confirmed as RVA-positive by the first round RT-PCR out of the twenty RVA antigen-positive samples that were examined. Based on RT-PCR assay, the prevalence of RVA which caused diarrhea in children less than five years was estimated at 4.25%. All the 17 stool samples that were confirmed RVA-positive by first round RT-PCR were successfully genotyped for VP7-G and VP4-P genes. Multiplex PCR revealed that G2[P6] was the most frequently found genotype combination (1.50%) rather than the G1[P8] (1.25%) which occurred most frequently worldwide. G9[P6] strains were responsible for 0.50% of RVA prevalence. Unusual RVA strains carrying genotype G2[P8] accounted for a prevalence of 0.50% while mixed infections caused by the G2G9[P6] strains also accounted for a prevalence of 0.50%.

وصف الملف: electronic resource

العلاقة: https://mid.journals.ekb.eg/article_302976_ffb9a36a5245bbf112d93ba5269f7110.pdfTest; https://doaj.org/toc/2682-4132Test; https://doaj.org/toc/2682-4140Test

الوصول الحر: https://doaj.org/article/a8ca549b257040ca8452cddb4cc91d1aTest

المؤلفون: Mariano Carossino, Maria Aldana Vissani, Maria E. Barrandeguy, Udeni B. R. Balasuriya, Viviana Parreño

المصدر: Viruses, Vol 16, Iss 1, p 130 (2024)

مصطلحات موضوعية: rotavirus, group A rotavirus, equine rotavirus A, One Health, public health, diarrhea, Microbiology, QR1-502

الوصف: Group A rotaviruses are a well-known cause of viral gastroenteritis in infants and children, as well as in many mammalian species and birds, affecting them at a young age. This group of viruses has a double-stranded, segmented RNA genome with high genetic diversity linked to point mutations, recombination, and, importantly, reassortment. While initial molecular investigations undertaken in the 1900s suggested host range restriction among group A rotaviruses based on the fact that different gene segments were distributed among different animal species, recent molecular surveillance and genome constellation genotyping studies conducted by the Rotavirus Classification Working Group (RCWG) have shown that animal rotaviruses serve as a source of diversification of human rotavirus A, highlighting their zoonotic potential. Rotaviruses occurring in various animal species have been linked with contributing genetic material to human rotaviruses, including horses, with the most recent identification of equine-like G3 rotavirus A infecting children. The goal of this article is to review relevant information related to rotavirus structure/genomic organization, epidemiology (with a focus on human and equine rotavirus A), evolution, inter-species transmission, and the potential zoonotic role of equine and other animal rotaviruses. Diagnostics, surveillance and the current status of human and livestock vaccines against RVA are also reviewed.

العلاقة: https://www.mdpi.com/1999-4915/16/1/130Test; https://doaj.org/toc/1999-4915Test; https://doaj.org/article/40c0e064060b45a0b0163d5916c6d49eTest

الإتاحة: https://doi.org/10.3390/v16010130Test
https://doaj.org/article/40c0e064060b45a0b0163d5916c6d49eTest

المؤلفون: Han Wu, Bingzhe Li, Ziping Miao, Linjie Hu, Lu Zhou, Yihan Lu

المصدر: BMC Genomics, Vol 23, Iss 1, Pp 1-10 (2022)

مصطلحات موضوعية: Group A rotavirus, P genotype, VP4, Complete coding sequence, Codon usage bias, Evolution, Biotechnology, TP248.13-248.65, Genetics, QH426-470

الوصف: Abstract Background Group A rotavirus (RVA) is a common causative agent of acute gastroenteritis in infants and young children worldwide. RVA P genotypes, determined by VP4 sequences, have been confirmed to infect humans and animals. However, their codon usage patterns that are essential to obtain insights into the viral evolution, host adaptability, and genetic characterization remained unclear, especially across animal hosts. Results We performed a comprehensive codon usage analysis of eight host-specific RVA P genotypes, including human RVA (P[4] and P[8]), porcine RVA (P[13] and P[23]), and zoonotic RVA (P[1], P[6], P[7] and P[19]), based on 233 VP4 complete coding sequences. Nucleotide composition, relative synonymous codon usage (RSCU), and effective number of codons (ENC) were calculated. Principal component analysis (PCA) based on RSCU values was used to explore the codon usage patterns of different RVA P genotypes. In addition, mutation pressure and natural selection were identified by using ENC-plot, parity rule 2 plot, and neutrality plot analyses. All VP4 sequences preferred using A/U nucleotides (A: 0.354-0.377, U: 0.267-0.314) than G/C nucleotides across genotypes. Similarly, majority of commonly used synonymous codons were likely to end with A/U nucleotides (A: 9/18-12/18, U: 6/18-9/18). In PCA, human, porcine, and zoonotic genotypes clustered separately in terms of RSCU values, indicating the host-specific codon usage patterns; however, porcine and zoonotic genotypes were partly overlapped. Human genotypes, P[4] and P[8], had stronger codon usage bias, as indicated by more over-represented codons and lower ENC, compared to porcine and zoonotic genotypes. Moreover, natural selection was determined to be a predominant driver in shaping the codon usage bias across the eight P genotypes. In addition, mutation pressure contributed to the codon usage bias of human genotypes. Conclusions Our study identified a strong codon usage bias of human RVA P genotypes attributable to both natural selection and mutation pressure, whereas similar codon usage bias between porcine and zoonotic genotypes predominantly attributable to natural selection. It further suggests possible cross-species transmission. Therefore, it warrants further surveillance of RVA P genotypes for early identification of zoonotic infection.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2164Test

الوصول الحر: https://doaj.org/article/371b72cf142540a09103cdd8013a7f9eTest

المؤلفون: Xuanpeng Wang, Jing Yu, Xin Zhang

المصدر: Frontiers in Cellular and Infection Microbiology, Vol 12 (2022)

مصطلحات موضوعية: intestinal pathogens, host immunity, yersinia, salmonella, zika virus, group A rotavirus, Microbiology, QR1-502

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fcimb.2022.1053792/fullTest; https://doaj.org/toc/2235-2988Test

الوصول الحر: https://doaj.org/article/09ba0b2870674389abfebe9c771c89e6Test

المؤلفون: Lv YJ, Hu QL, Huang R, Zhang L, Wu LF, Fu S

المصدر: International Journal of General Medicine, Vol Volume 14, Pp 3611-3617 (2021)

مصطلحات موضوعية: group a rotavirus, serum amyloid a, high sensitivity, c-reactive protein, white blood cell, diagnostic efficacy, Medicine (General), R5-920

الوصف: Yin-Jiang Lv, Qi-Lei Hu, Rong Huang, Liang Zhang, Li-Feng Wu, Shui Fu Department of Clinical Laboratory, The First People’s Hospital of Yuhang District, Hangzhou, Hangzhou, Zhejiang, 311100, People’s Republic of ChinaCorrespondence: Shui FuDepartment of Clinical Laboratory, The First People’s Hospital of Yuhang District, Hangzhou, No. 369 Yingbin Road of Nanyuan Street, Yuhang District, Hangzhou, 311100, People’s Republic of ChinaTel +86 571 89369404Email fushuiolo@163.comObjective: This study explores the significance of serum amyloid A (SAA), C-reactive protein (CRP), and white blood cell (WBC) in the diagnosis and treatment of diarrhea in infants.Methods: Specimens were collected from 126 children with diarrhea and 66 healthy children undergoing health examination. According to the results of stool culture and rotavirus (RV) antigen, these children were divided into three groups: rotavirus group (70 cases), bacterial infection (56 cases), and control groups (66 cases). On the fourth day of admission, children in the RV group underwent stool culture again. Based on the subsequent results, they were further divided into two groups, ie, no secondary bacterial infection and secondary bacterial infection groups. The levels of RV antigen, bacterial antigen, SAA, CRP, and WBC were detected in all children. Then, ROC curve analysis was performed to determine the diagnostic efficacy of SAA, CRP and WBC.Results: The levels of SAA, CRP, and WBC for the RV group were lower than those of the bacterial infection group, but higher compared with the control group (P< 0.05). The diagnostic efficacy of SAA was higher than that of CRP and WBC, with the area under the curve of 0.876, 0.803, and 0.765, respectively. The positive and negative predictive values, specificity, and sensitivity of SAA were slightly better compared with CRP and WBC. The SAA, CRP, and WBC levels of children with a bacterial infection in the RV group on the fourth and seventh days after admission were also significantly higher compared with children without bacterial infection.Conclusion: Serum amyloid A, CRP, and WBC levels had a high value in the differential diagnosis of infantile diarrhea. As such, they can be used in the early diagnosis and curative efficacy assessment of children with diarrhea.Keywords: group A rotavirus, serum amyloid A, C-reactive protein, white blood cell, diagnostic efficacy

وصف الملف: electronic resource

العلاقة: https://www.dovepress.com/the-diagnostic-and-therapeutic-value-of-the-detection-of-serum-amyloid-peer-reviewed-fulltext-article-IJGMTest; https://doaj.org/toc/1178-7074Test

الوصول الحر: https://doaj.org/article/333c9b470af240eda9785c7b64eee7dcTest

المؤلفون: Lifeng Zhao, Xiaohong Shi, Dequan Meng, Jiane Guo, Yiping Li, Lirong Liang, Xiaofang Guo, Ran Tao, Xiaohua Zhang, Ruihong Gao, Li Gao, Jitao Wang

المصدر: BMC Infectious Diseases, Vol 21, Iss 1, Pp 1-14 (2021)

مصطلحات موضوعية: Group a rotavirus, Active surveillance, Acute gastroenteritis, Genotypes, Infectious and parasitic diseases, RC109-216

الوصف: Abstract Background Group A rotavirus (RVA), despite being a leading cause of gastroenteritis in infants and young children, is less studied in Shanxi Province, China. The current study was conducted to determine the prevalence and genetic characterization of RVA in hospitalized children younger than 10 years of age with the diagnosis of acute gastroenteritis in Shanxi Province, China. Methods A hospital-based active surveillance of rotavirus gastroenteritis was conducted at Children’s Hospital of Shanxi from Jan 1, 2015, through Dec 31, 2019. Rotavirus was detected in stool samples by real-time quantitative reverse transcription PCR (qRT-PCR). G- and P-genotypes were determined by reverse transcription PCR (RT-PCR) and nucleotide sequencing. Results A total of 961 children younger than 10 years of age was enrolled over the study period, of whom 183 (19.0%) were positive for RVA. The highest RVA-infection frequency (23.7%) was found among children aged 12–23 months, and the seasonal peak was in December. G9P[8] was most prevalent (76.0%), followed by G3P[8] (7.1%), G2P[4] (3.3%), G1P[8] (0.5%) and G9P[4] (0.5%). Conclusions These results report for the first time that RVA was one of the main causes of severe infectious gastroenteritis in children, and a high proportion of G9P[8] strains circulating in most areas of Shanxi Province. While the protective efficacy of the rotavirus vaccines has been demonstrated against G9P[8] strains, our results highlight that the dominant strains have not been effectively controlled in China.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2334Test

الوصول الحر: https://doaj.org/article/992f5b329f7c4e3eacc5ff565f3ebddeTest

المؤلفون: Xiaozhou Kuang, Xiaohuan Gong, Xi Zhang, Hao Pan, Zheng Teng

المصدر: BMC Infectious Diseases, Vol 20, Iss 1, Pp 1-11 (2020)

مصطلحات موضوعية: Group A rotavirus, Acute gastroenteritis, Outpatients, Genotype, Antibiotics, Fecal shedding, Infectious and parasitic diseases, RC109-216

الوصف: Abstract Background Group A Rotavirus (RVA), despite being an important pathogen in hospitalized children, is less studied in pediatric outpatients, and even rarely investigated in adults. This study aims to understand the genetic diversity of RVA in outpatients across all age groups in Shanghai, and thus providing a molecular basis for vaccine implementation and evaluation. Methods Stool samples were first screened by Real-time Reverse Transcription Polymerase Chain Reaction (rRT-PCR). RVA genotyping was performed through the amplification of partial VP7 and VP4 gene. Strains of interest were further sequenced and analyzed using MEGA 6.0. Results Four thousand nine hundred one samples were collected, from which 7.61% (373 cases) were screened positive for RVA. RVA prevalence was higher in children (9.30%) than in adults (7.21%) (χ2 = 4.72, P

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s12879-020-05279-xTest; https://doaj.org/toc/1471-2334Test

الوصول الحر: https://doaj.org/article/190bd3a890304d4dafd997ddd86e2463Test

المؤلفون: Mwangi, Peter N., Page, Nicola Anne, Seheri, Mapaseka L., Mphahlele, M., Nadan, Sandrama, Esona, Mathew D., Kumwenda, Benjamin, Kamng’ona, Arox W., Donato, Celeste M., Steele, Duncan A., Ndze, Valantine N., Dennis, Francis E., Jere, Khuzwayo C., Nyaga, Martin M.

مصطلحات موضوعية: G2P[4] Group A rotavirus strains, Rotavirus, Sub-lineages, Whole-genome analysis

الوصف: DATA STATEMENT : All supporting data, code and protocols have been provided within the article or through supplementary data files. ; The transient upsurge of G2P[4] group A rotavirus (RVA) after Rotarix vaccine introduction in several countries has been a matter of concern. To gain insight into the diversity and evolution of G2P[4] strains in South Africa pre- and post-RVA vaccination introduction, whole-genome sequencing was performed for RVA positive faecal specimens collected between 2003 and 2017 and samples previously sequenced were obtained from GenBank (n=103; 56 pre- and 47 post-vaccine). Pre-vaccine G2 sequences predominantly clustered within sub-lineage IVa-1. In contrast, post-vaccine G2 sequences clustered mainly within sub-lineage IVa-3, whereby a radical amino acid (AA) substitution, S15F, was observed between the two sub-lineages. Pre-vaccine P[4] sequences predominantly segregated within sub-lineage IVa while post-vaccine sequences clustered mostly within sub-lineage IVb, with a radical AA substitution R162G. Both S15F and R162G occurred outside recognised antigenic sites. The AA residue at position 15 is found within the signal sequence domain of Viral Protein 7 (VP7) involved in translocation of VP7 into endoplasmic reticulum during infection process. The 162 AA residue lies within the hemagglutination domain of Viral Protein 4 (VP4) engaged in interaction with sialic acid-containing structure during attachment to the target cell. Free energy change analysis on VP7 indicated accumulation of stable point mutations in both antigenic and non-antigenic regions. The segregation of South African G2P[4] strains into pre- and post-vaccination sub-lineages is likely due to erstwhile hypothesized stepwise lineage/sub-lineage evolution of G2P[4] strains rather than RVA vaccine introduction. Our findings reinforce the need for continuous whole-genome RVA surveillance and investigation of contribution of AA substitutions in understanding the dynamic G2P[4] epidemiology. ; The Bill and Melinda Gates ...

وصف الملف: application/pdf; application/vnd.openxmlformats-officedocument.spreadsheetml.sheet

العلاقة: Mwangi, P.N., Page, N.A., Seheri, M.L. et al. 2022, 'Evolutionary changes between pre- and post-vaccine South African group A G2P[4] rotavirus strains, 2003–2017', Microbial Genomics, vol. 2022, no. 8, pp. 1-14. DOI 10.1099/mgen.0.000809.; http://hdl.handle.net/2263/92310Test

الإتاحة: https://doi.org/10.1099/mgen.0.000809Test
http://hdl.handle.net/2263/92310Test

المؤلفون: Ruixue Jiao, Zhaoyang Ji, Xiaoyuan Zhu, Hongyan Shi, Jianfei Chen, Da Shi, Jianbo Liu, Zhaoyang Jing, Jiyu Zhang, Liaoyuan Zhang, Shufeng Feng, Xin Zhang, Li Feng

المصدر: Animals; Volume 12; Issue 21; Pages: 2951

مصطلحات موضوعية: emerging, co-infection, porcine group C rotavirus, recombination

جغرافية الموضوع: agris

الوصف: Swine enteric disease is the predominant cause of morbidity and mortality, and viral species involved in swine enteric disease include rotaviruses and coronaviruses, among others. Awareness of the circulating porcine rotavirus group C (PoRVC) in pig herds is critical to evaluate the potential impact of infection. At present, due to the lack of disease awareness and molecular diagnostic means, the research on RVC infection in China is not well-studied. In this study, diarrhea samples collected from pig farms were detected positive for RVC by PCR, and the full-length RVC was not previously reported for Chinese pig farms. This rotavirus strain was designated as RVC/Pig/CHN/JS02/2018/G6P6. A natural recombination event was observed with breakpoints at nucleotides (nt) 2509 to 2748 of the VP2 gene. Phylogenetic analysis based on nsp1 revealed that a new branch A10 formed. Collectively, our data suggest a potentially novel gene recombination event of RVC in the VP2 gene. These findings provide a new insight into the evolution of the rotavirus.

وصف الملف: application/pdf

العلاقة: Pigs; https://dx.doi.org/10.3390/ani12212951Test

الإتاحة: https://doi.org/10.3390/ani12212951Test