المؤلفون: DiFrancesco, Jacopo C., Tabaee Damavandi, Payam, Pretnar‐Oblak, Janja, Frol, Senta, de la Riva Juez, Patricia, Albajar Gomez, Ines, Jensen‐Kondering, Ulf

المصدر: European Journal of Neurology ; ISSN 1351-5101 1468-1331

الإتاحة: https://doi.org/10.1111/ene.16366Test

المؤلفون: Diaz Alarcón, Jhonathan A., Fonseca Alfonso, Paola M., Vergara Gómez, Inés, Díaz Lagos, Mercedes, Videira Quintela, Diogo Manuel, Montalvo García, Gemma

المساهمون: Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química

مصطلحات موضوعية: Potentially hazardous elements, GIS analysis, Soil contamination, Geochemical background, Química, Chemistry

الوصف: In the industrial corridor of Boyacá, Colombia, population growth is accompanied by anthropogenic activities such as industrial operations, vehicle exhaust fumes, mining, smelting, atmospheric deposition, and excessive use of chemical products to promote crop growth. These activities are known to have a significant impact on urban and rural soils, contributing significantly to elevated concentrations of potentially hazardous elements in the environment. This industrial corridor is an area of economic and social development that needs to provide reference information that will allow us to know the state of soil quality to preserve and manage the public and geoenvironmental health of this region. Anthropogenic activities have contributed to the accumulation of potentially hazardous elements in the environment, affecting various levels of life and creating risks with economic and social implications. However, igneous activity or detrital deposition also enriches soils and creates geochemical anomalies in specific locations. In these cases, the identification of potentially hazardous elements involves the determination of likely sources of contamination and their relationship to the geological setting. In this study, the concentrations of As, Cd, Pb, Mn, Fe, Zn, Hg, Cu and Ni were determined in eighty-one soil samples from the Boyacá industrial corridor (Colombia). The sequential trend of the concentrations of potentially hazardous elements was as follows: Fe > Mn > Zn > Ni > Cu> Pb > As > Cd > Hg. Furthermore, the application of spatial analysis criteria in GIS software with multivariate statistical tools and geochemical indices allowed the identification of anthropogenic and geogenic sources. Most of the potentially hazardous elements were found in soils exposed to industrial and agricultural activities, except for iron. This element showed low variability in all samples, regardless of the geological formations. Due to the lack of reference values for potentially hazardous elements in ...

وصف الملف: application/pdf

العلاقة: Ecotoxicology and Environmental Safety, 2024, v. 269, p. 115725-115737; http://hdl.handle.net/10017/60709Test; https://doi.org/10.1016/j.ecoenv.2023.115725Test; AR/0000047202; Ecotoxicology and Environmental Safety; 269; 115737; 115725

الإتاحة: https://doi.org/10.1016/j.ecoenv.2023.115725Test
http://hdl.handle.net/10017/60709Test

المؤلفون: Felgendreff, Philipp, Hosseiniasl, Seyed M., Felgendreff, Lisa, Amiot, Bruce P., Minshew, Anna, Ahmadzada, Boyukkhanim, Qu, Zhi, Wilken, Silvana, Arribas Gomez, Ines, Nyberg, Scott L., Cook, Casey N.

المصدر: Frontiers in Medicine ; volume 11 ; ISSN 2296-858X

مصطلحات موضوعية: General Medicine

الوصف: Introduction Acute liver failure (ALF) is defined as acute loss of liver function leading to hepatic encephalopathy associated with a high risk of patient death. Brain injury markers in serum and tissue can help detect and monitor ALF-associated brain injury. This study compares different brain injury parameters in plasma and tissue along with the progression of ALF. Method ALF was induced by performing an 85% liver resection. Following the resection, animals were recovered and monitored for up to 48 h or until reaching the predefined endpoint of receiving standard medical therapy (SMT). Blood and serum samples were taken at T baseline , T 24 , and upon reaching the endpoint (T end ). Control animals were euthanized by exsanguination following plasma sampling. Postmortem brain tissue samples were collected from the frontal cortex (FCTx) and cerebellum (Cb) of all animals. Glial fibrillary acidic protein (GFAP) and tau protein and mRNA levels were quantified using ELISA and qRT-PCR in all plasma and brain samples. Plasma neurofilament light (NFL) was also measured using ELISA. Results All ALF animals ( n = 4) were euthanized upon showing signs of brain herniation. Evaluation of brain injury biomarkers revealed that GFAP was elevated in ALF animals at T 24h and T end , while Tau and NFL concentrations were unchanged. Moreover, plasma glial fibrillary acidic protein (GFAP) levels were negatively correlated with total protein and positively correlated with both aspartate transaminase (AST) and alkaline phosphatase (AP). Additionally, lower GFAP and tau RNA expressions were observed in the FCTx of the ALF group but not in the CB tissue. Conclusion The current large animal study has identified a strong correlation between GFAP concentration in the blood and markers of ALF. Additionally, the protein and gene expression analyses in the FCTx revealed that this area appears to be susceptible, while the CB is protected from the detrimental impacts of ALF-associated brain swelling. These results warrant further studies to ...

الإتاحة: https://doi.org/10.3389/fmed.2024.1363979Test

المؤلفون: Pérez-Camacho, Paola Marsela, Patiño-Niño, Jaime A., Restrepo Cedeño, Alejandro, Gómez, Inés Elvira, Sandoval-Calle, Lina M.

المساهمون: Fundacion Valle del Lili

المصدر: Travel Medicine and Infectious Disease ; volume 59, page 102706 ; ISSN 1477-8939

مصطلحات موضوعية: Infectious Diseases, Public Health, Environmental and Occupational Health

الإتاحة: https://doi.org/10.1016/j.tmaid.2024.102706Test
https://api.elsevier.com/content/article/PII:S1477893924000206?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S1477893924000206?httpAccept=text/plainTest

المؤلفون: Diaz Lagos, Mercedes, López Aparicio, Johana Hosyris, Alvarado Sánchez, Juliana Andrea, Vergara Gómez, Inés, Díaz Alarcón, Jhonathan Andrés

المصدر: UPTC Editorial; ; Editorial UPTC

الوصف: In this research work, investigate on physical, chemical, mineralogical and petrographic properties of clay mineral resources converges to contribute to solve the problems of artisanal pottery production of the Association of Women Miners and Entrepreneurs (ASOAMME) of the “Pantanitos” sidewalk in Sogamoso, Colombia. The composition of the clays, their thermal, plastic and mineralogical properties are determined from samples from the three mining titles exploited by the Association. X-ray fluorescence (FRX) analyses determine the preliminary chemical composition and lead the definition of mineral groups identified by X-ray diffraction (DRX) and Fourier transform infrared spectroscopy (FTIR). The interpretation of mineral phases is complemented by optical microscopy to identify materials such as cryptocrystalline quartz and some minerals with granulometries greater than 5 μm. Scanning electron microscopy (SEM) is also implemented to recognize the laminar structures of kaolinite and validate the results of analytical techniques. The thermal behavior was performed through of a thermogravimetric analysis (TGA) that was adjusted to the mineralogical properties of the clays and the infrastructure in the pottery workshop of the Association. The results point out that the clays used by the Association present differential chemical contents of kaolinite and montmorillonite, which modifies their properties during cooking. It is concluded that mixtures between the different levels of clay are not recommended for making ceramic pieces. The authors make a diagnosis and suggest about the current artisanal production process, without affecting traditional ones. Aspects such as the handling of humidity, cooking temperature controls and the convenience of using fixtures or curvatures are addressed. ; En esta obra de divulgación converge la investigación sobre propiedades físicas, químicas, mineralógicas y petrográficas de recursos minerales arcillosos para contribuir en las problemáticas de producción de alfarería artesanal de ...

وصف الملف: Digital (DA)

العلاقة: https://librosaccesoabierto.uptc.edu.co/index.php/editorial-uptc/catalog/view/390/285/7621Test; https://librosaccesoabierto.uptc.edu.co/index.php/editorial-uptc/catalog/view/390/285/7622Test; https://librosaccesoabierto.uptc.edu.co/index.php/editorial-uptc/catalog/view/390/285/7623Test; https://librosaccesoabierto.uptc.edu.co/index.php/editorial-uptc/catalog/view/390/285/7624Test; https://librosaccesoabierto.uptc.edu.co/index.php/editorial-uptc/catalog/view/390/285/7625Test; https://librosaccesoabierto.uptc.edu.co/index.php/editorial-uptc/catalog/view/390/285/7626Test; https://librosaccesoabierto.uptc.edu.co/index.php/editorial-uptc/catalog/book/390Test

الإتاحة: https://librosaccesoabierto.uptc.edu.co/index.php/editorial-uptc/catalog/book/390Test

المؤلفون: Baniahmad, Seyed Farzad, Oliverio, Romane, Obregón Gómez, Inés, Robert, Alma, Lenferink, Anne, Pazos, Elena, Virgilio, Nick, Banquy, Xavier, De Crescenzo, Gregory, Durocher, Yves

مصطلحات موضوعية: Affinity, Coiled-coils, Hydrogels, Monoclonal antibodies, Sustained release

الوصف: [Abstract] Long-term delivery is a successful strategy used to reduce the adverse effects of monoclonal antibody (mAb)-based treatments. Macroporous hydrogels and affinity-based strategies have shown promising results in sustained and localized delivery of the mAbs. Among the potential tools for affinity-based delivery systems, the de novo designed Ecoil and Kcoil peptides are engineered to form a high-affinity, heterodimeric coiled-coil complex under physiological conditions. In this study, we created a set of trastuzumab molecules tagged with various Ecoil peptides and evaluated their manufacturability and characteristics. Our data show that addition of an Ecoil tag at the C-termini of the antibody chains (light chains, heavy chains, or both) does not hinder the production of chimeric trastuzumab in CHO cells or affect antibody binding to its antigen. We also evaluated the influence of the number, length, and position of the Ecoil tags on the capture and release of Ecoil-tagged trastuzumab from macroporous dextran hydrogels functionalized with Kcoil peptide (the Ecoil peptide-binding partner). Notably, our data show that antibodies are released from the macroporous hydrogels in a biphasic manner; the first phase corresponding to the rapid release of residual, unbound trastuzumab from the macropores, followed by the affinity-controlled, slow-rate release of antibodies from the Kcoil-functionalized macropore surface. ; The work was supported by the Agencia Estatal de Investigación [RYC2019-027199-I]; Canada First Research Excellence Fund [TransMedTech]; Canada Research Chairs European Research Council [851179]; Fonds de recherche du Québec – Nature et technologies Natural Sciences and Engineering Research Council of Canada [NSERC-CREATE PrEEmiuM program]; Xunta de Galicia [ED431C 2018/39, ED431C 2022/39, 508/2020]; Xunta de Galicia [ED481A-2021/008] ; Xunta de Galicia; ED431C 2018/39 ; Xunta de Galicia; ED431C 2022/39 ; Xunta de Galicia; ED481A-2021/008

العلاقة: info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RYC2019-027199-I/ES/; info:eu-repo/grantAgreement/EC/H2020/851179; https://doi.org/10.1080/19420862.2023.2218951Test; Seyed Farzad Baniahmad, Romane Oliverio, Ines Obregon-Gomez, Alma Robert, Anne E.G. Lenferink, Elena Pazos, Nick Virgilio, Xavier Banquy, Gregory De Crescenzo & Yves Durocher (2023) Affinity-controlled capture and release of engineered monoclonal antibodies by macroporous dextran hydrogels using coiled-coil interactions, mAbs, 15:1, DOI:10.1080/19420862.2023.2218951; http://hdl.handle.net/2183/34206Test

الإتاحة: https://doi.org/10.1080/19420862.2023.2218951Test
http://hdl.handle.net/2183/34206Test

المؤلفون: Torrejon-Rodriguez, Laura, Pinilla-Gonzalez, Alejandro, Lara Cantón, Inmaculada, Albiach-Delgado, Abel, Cascant-Vilaplana, Mari Merce, Cernada, María, Kuligowski, Julia, Solves Alcaina, Maria Pilar, Gómez, Inés, Vento, Maximo, Aguar Carrascosa, Marta

المصدر: Frontiers in Pediatrics ; volume 11 ; ISSN 2296-2360

مصطلحات موضوعية: Pediatrics, Perinatology and Child Health

الوصف: Background Currently, the treatment of anemia in preterm infants is based on packed red blood cell (RBC) transfusions from adult donors. Oxygen (O2) is mainly transported to the tissues bound to hemoglobin (Hb). In extremely low gestational age neonates (ELGANs), fetal hemoglobin (HbF), which has a higher affinity for O2, represents up to 95% of circulating hemoglobin. During the first month of life, the majority of ELGANs will require an adult-donor RBC transfusion causing HbF levels to rapidly drop. HbA releases 50% more oxygen in peripheral tissues than HbF. Increased release of O2 in the retina is one of the main factors related to the development of retinopathy of prematurity (ROP). Collecting umbilical cord blood and using autologous umbilical cord whole blood (UCB) transfusions would contribute to maintaining physiological HbF concentrations in newborns and avoid oxygen-in-excess derived damage. Methods This is a randomized, double-blinded, multicenter clinical trial. ELGANs ≤28 weeks of gestational age will be randomized 1:1 to receive an autologous umbilical cord blood transfusion (intervention arm) or standard transfusion of packed RBC from an adult donor (control arm) to assess ROP development. Assuming a 50% reduction in ROP incidence, 134 patients (67 per group) will be recruited. When blood transfusion is indicated, the Blook Bank will supply UCB or RCB according to the patient's group. The primary endpoint is the incidence of any ROP. Secondary endpoints are assessessment of treatment safety, results of biomarkers related to ROP and its chronology, and urine oxidative stress markers. In addition, the cellular composition of umbilical cord blood and its relationship with prematurity-related pathologies will be analyzed. All patients will be followed-up to 24 months of corrected age to evaluate their neurodevelopment. Discussion ROP is a major cause of irreversible blindness in preterm newborns. Transfusions with adult donor blood can lead to complications, including ROP. UCB transfusions offer ...

الإتاحة: https://doi.org/10.3389/fped.2023.1269797Test

المؤلفون: Cordón, Lourdes, Chorão, Pedro, Martín-Herreros, Beatriz, Montoro, Juan, Balaguer, Aitana, Guerreiro, Manuel, Villalba, Marta, Facal, Ana, Asensi, Pedro, Solves, Pilar, Gómez, Inés, Santiago, Marta, Lamas, Brais, Bataller, Ana, Granados, Pablo, Sempere, Amparo, Sanz, Guillermo F., Sanz, Miguel A., Sanz, Jaime

المصدر: Annals of Hematology; Jul2024, Vol. 103 Issue 7, p2475-2484, 10p

مصطلحات موضوعية: CORD blood transplantation, HEMATOLOGIC malignancies, T cells, KILLER cells, ADULTS

مستخلص: This study aimed to investigate the kinetics of immune recovery following umbilical cord blood transplantation (UCBT) in adults who received a myeloablative conditioning (MAC) regimen and antithymocyte globulin (ATG). While the immune recovery kinetics has been extensively studied in pediatric UCBT recipients, limited data exist for adults. We conducted a comprehensive analysis of 221 consecutive adult patients who underwent UCBT with MAC and ATG at a single institution. Our objective was to evaluate the influence of patient, disease, and transplant factors, along with acute graft-versus-host disease (aGVHD), on immune reconstitution and overall survival. Our findings confirm a delayed recovery of T cells, while B and NK cell reconstitution exhibited rapid progress, with NK cell counts reaching normal levels within 3 months post-transplantation and B cells within 6 months. Within CD3⁺ T cells, CD8⁺ T cells also experienced a delayed recovery (12 months), but to a lesser extent compared to CD4⁺ T cells (18 months). Delayed immune recovery of T-cell subsets was associated with the development of aGVHD grade II-IV, older age, CMV negativity, and a female donor. Patients with lymphoproliferative diseases showed slower NK cell recovery. Our study demonstrates that adult patients undergoing MAC with ATG and receiving a single unit UCBT for hematologic malignancies experienced rapid reconstitution of NK and B cells. However, T cell recovery, particularly CD4⁺ T cells, was significantly delayed. To enhance T cell recovery, it may be crucial to consider UCB units with higher cellularity and optimize ATG doses in conditioning. [ABSTRACT FROM AUTHOR]

: Copyright of Annals of Hematology is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Chorão, Pedro, Henriques, Marta, Villalba, Marta, Montoro, Juan, Balaguer-Roselló, Aitana, González, Eva María, Gómez, María Dolores, Gómez, Inés, Solves, Pilar, Santiago, Marta, Asensi, Pedro, Lamas, Brais, Bataller, Ana, Granados, Pablo, Eiris, Juan, Martínez, David, Louro, Alberto, Rebollar, Paula, Perla, Aurora, Salavert, Miguel, de la Rubia, Javier, Sanz, Miguel Ángel, Sanz, Jaime

المصدر: Transplantation and Cellular Therapy; May 2024, Vol. 30 Issue: 5 p538.e1-538.e10, 48430p

مستخلص: • First CMV reactivations in HSCT with PTCy without letermovir prophylaxis occurred in 46% of patients.• The reactivation rate was 73% for CMV seropositive recipients and 49% for CMV-seropositive donors.• Risk factors for reactivation were CMV seropositivity, haploidentical HSCT, older patient, and grade II-IV acute GVHD.• Second and third CMV reactivations occurred in 13% and 4.4% of patients, independent of donor type.

المؤلفون: Sanz, Miguel Ángel, Montoro, Juan, Balaguer-Roselló, Aitana, Chorão, Pedro, Villalba, Marta, Gómez, Inés, Solves, Pilar, Santiago, Marta, Asensi, Pedro, Lamas, Brais, Bataller, Ana, Granados, Pablo, Eiris, Juan, Martinez, David, Lloret, Pilar, Louro, Alberto, Rebollar, Paula, Perla, Aurora, de la Rubia, Javier, Sanz, Jaime

المصدر: Bone Marrow Transplant ; ISSN:1476-5365

الوصف: This 45-year study (1978-2022) at a single institution evaluated HSCT outcomes and complications, emphasizing recent advances, with to provide insights into HSCT's evolving field and ongoing efforts to enhance patient outcomes. Involving 1707 patients, the study revealed an initial phase (1978-1987) with a limited activity that yielded modest outcomes, a nearly three-decade span (1988-2016) with a substantial increase in transplant activity, emphasizing umbilical cord blood transplantation (UCBT) for patients lacking a suitable matched sibling donor. In addition to a gradual increase in recipient age, significant improvement in outcomes emerged in the recent period (2017-2022), marked by UCBT replacement with haploidentical transplants, introduction of PTCY-based GVHD prophylaxis for all type of transplants, and increased use of conditioning regimens with thiotepa, busulfan, and fludarabine. In this period, reductions in GVHD, non-relapse mortality, and relapse rates significantly contributed to improved overall survival, event-free survival, and GVHD-free/relapse-free survival. The study identified specific factors, including GVHD prophylaxis and donor selection changes, associated with these positive trends. This four-decade study provides a unique perspective on allogeneic HSCT, showcasing the dynamic evolution of transplantation practices and their impact on outcomes, offering valuable insights for personalized treatment approaches and emphasizing continual innovation in this critical therapeutic modality.

العلاقة: https://doi.org/10.1038/s41409-024-02319-xTest; https://pubmed.ncbi.nlm.nih.gov/38918495Test

الإتاحة: https://doi.org/10.1038/s41409-024-02319-xTest
https://pubmed.ncbi.nlm.nih.gov/38918495Test