المؤلفون: Laurent Misery, Markéta Saint Aroman, Asmaa Zkik, Alain Briant, Ludovic Martin, Michèle-Léa Sigal, Christine Bodemer, Gérard Guillet, Martine Bagot, Charles Taïeb

المصدر: Acta Dermato-Venereologica, Vol 97, Iss 8, Pp 986-988 (2017)

مصطلحات موضوعية: Dermatology, RL1-803

وصف الملف: electronic resource

العلاقة: https://www.medicaljournals.se/acta/content/html/10.2340/00015555-2694Test; https://doaj.org/toc/0001-5555Test; https://doaj.org/toc/1651-2057Test

الوصول الحر: https://doaj.org/article/b3b25efbb4b6400591d819456861e7cdTest

المؤلفون: Elodie Couderc, Franck Morel, Pierre Levillain, Amandine Buffière-Morgado, Magalie Camus, Camille Paquier, Charles Bodet, Jean-François Jégou, Mathilde Pohin, Laure Favot, Martine Garcia, Vincent Huguier, Jiad Mcheik, Corinne Lacombe, Hans Yssel, Gérard Guillet, François-Xavier Bernard, Jean-Claude Lecron

المصدر: PLoS ONE, Vol 12, Iss 7, p e0181486 (2017)

مصطلحات موضوعية: Medicine, Science

الوصف: Acute-serum Amyloid A (A-SAA), one of the major acute-phase proteins, is mainly produced in the liver but extra-hepatic synthesis involving the skin has been reported. Its expression is regulated by the transcription factors NF-κB, C/EBPβ, STAT3 activated by proinflammatory cytokines.We investigated A-SAA synthesis by resting and cytokine-activated Normal Human Epidermal Keratinocytes (NHEK), and their inflammatory response to A-SAA stimulation. A-SAA expression was also studied in mouse skin and liver in a model mimicking psoriasis and in the skin and sera of psoriatic and atopic dermatitis (AD) patients.NHEK were stimulated by A-SAA or the cytokines IL-1α, IL-17A, IL-22, OSM, TNF-α alone or in combination, previously reported to reproduce features of psoriasis. Murine skins were treated by imiquimod cream. Human skins and sera were obtained from patients with psoriasis and AD. A-SAA mRNA was quantified by RT qPCR. A-SAA proteins were dosed by ELISA or immunonephelemetry assay.IL-1α, TNF-α and mainly IL-17A induced A-SAA expression by NHEK. A-SAA induced its own production and the synthesis of hBD2 and CCL20, both ligands for CCR6, a chemokine receptor involved in the trafficking of Th17 lymphocytes. A-SAA expression was increased in skins and livers from imiquimod-treated mice and in patient skins with psoriasis, but not significantly in those with AD. Correlations between A-SAA and psoriasis severity and duration were observed.Keratinocytes could contribute to psoriasis pathogenesis via A-SAA production, maintaining a cutaneous inflammatory environment, activating innate immunity and Th17 lymphocyte recruitment.

وصف الملف: electronic resource

العلاقة: http://europepmc.org/articles/PMC5510841?pdf=renderTest; https://doaj.org/toc/1932-6203Test

الوصول الحر: https://doaj.org/article/3f290c89fca648e39fea7fd29d34aa73Test

المؤلفون: Hanitriniaina Rabeony, Isabelle Petit-Paris, Julien Garnier, Christine Barrault, Nathalie Pedretti, Karline Guilloteau, Jean-François Jegou, Gérard Guillet, Vincent Huguier, Jean-Claude Lecron, François-Xavier Bernard, Franck Morel

المصدر: PLoS ONE, Vol 9, Iss 7, p e101937 (2014)

مصطلحات موضوعية: Medicine, Science

الوصف: Keratinocyte differentiation program leading to an organized epidermis plays a key role in maintaining the first line of defense of the skin. Epidermal integrity is regulated by a tight communication between keratinocytes and leucocytes, particularly under cytokine control. Imbalance of the cytokine network leads to inflammatory diseases such as psoriasis. Our attempt to model skin inflammation showed that the combination of IL-17A, IL-22, IL-1α, OSM and TNFα (Mix M5) synergistically increases chemokine and antimicrobial-peptide expression, recapitulating some features of psoriasis. Other characteristics of psoriasis are acanthosis and down-regulation of keratinocyte differentiation markers. Our aim was to characterize the specific roles of these cytokines on keratinocyte differentiation, and to compare with psoriatic lesion features. All cytokines decrease keratinocyte differentiation markers, but IL-22 and OSM were the most powerful, and the M5 strongly synergized the effects. In addition, IL-22 and OSM induced epidermal hyperplasia in vitro and M5 induced epidermal thickening and decreased differentiation marker expression in a mouse model, as observed in human psoriatic skin lesions. This study highlights the precise role of cytokines in the skin inflammatory response. IL-22 and OSM more specifically drive epidermal hyperplasia and differentiation loss while IL-1α, IL-17A and TNFα were more involved in the activation of innate immunity.

وصف الملف: electronic resource

العلاقة: http://europepmc.org/articles/PMC4092099?pdf=renderTest; https://doaj.org/toc/1932-6203Test

الوصول الحر: https://doaj.org/article/bdd797a88a164d73ada9d21afd75f734Test

المؤلفون: P.-A. Becherel, Michèle Debons-Peyroutet, Jean-Philippe Lacour, B. Pelvet, M. Ruer-Mulard, Audrey Lamirand, Frédéric Bérard, Pauline Pralong, Matthieu Greco, Françoise Giordano-Labadie, Gérard Guillet, Omar Outtas

المصدر: European Journal of Dermatology. 31:217-224

مصطلحات موضوعية: Adult, Male, medicine.medical_specialty, Drug Resistance, Efficiency, Omalizumab, Dermatology, Disease, Severity of Illness Index, Treatment satisfaction, Patient satisfaction, Cost of Illness, Drug Therapy, Quality of life, Internal medicine, Anti-Allergic Agents, medicine, Humans, Chronic Urticaria, Patient Reported Outcome Measures, Prospective Studies, Chronic urticaria, Angioedema, Adult patients, business.industry, Middle Aged, Patient Satisfaction, Histamine H1 Antagonists, Quality of Life, Health Resources, Female, France, Sick Leave, medicine.symptom, business, Follow-Up Studies, medicine.drug

الوصف: It is important to assess the burden of chronic urticaria (CU) with real-life studies. The AWARE study was performed in 36 countries over two years in CU patients resistant to H1-antihistamines.To correlate patient-reported outcomes and available therapeutic options in CU patients.The AWARE study was a prospective, non-interventional, international study that included adult patients who have had H1-antihistamine-resistant CU for at least two months. The primary endpoints were the evolution of disease activity (UAS7), urticaria control (UCT), dermatological quality of life (DLQI) and treatment satisfaction (visual analogic scale) during a two-year follow-up. The data from French centres are reported.Ninety-two patients were included (mean age: 47.8 years; women: 70.7%; mean disease duration: 6.5 years; angioedema: 34.1%). The percentage of patients with CU treatment increased from 56.5% at inclusion to 86.0% after two years (for patients with non-sedative H1-antihistamines from 52.2% to 74.4%, and omalizumab from 2.2% to 25.6%). During the follow-up, the percentage of patients with UAS7 score6 increased from 12.5% to 60.9%, and patients with well-controlled CU (UCT score12) increased from 11.1% to 62.2%. The negative impact on quality of life (DLQI10) decreased from 34.1% to 10.5%. The mean score of patient satisfaction for treatment increased from 4.6 to 7.6.The management of CU patients resistant to H1-antihistamines was not optimal at inclusion with uncontrolled disease, impaired quality of life and insufficient treatment. After a two-year follow-up, disease symptoms and quality of life improved, but the therapeutic management could be further optimized.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::074b0f19e4abb6ea0ed9e3f53a6e7d5eTest
https://doi.org/10.1684/ejd.2021.4023Test

المؤلفون: Christine Bodemer, Gerard Guillet, Frederic Cambazard, Franck Boralevi, Stefania Ballarini, Christian Milliet, Paola Bertuccio, Carlo La Vecchia, Jean-François Bach, Yves de Prost

المصدر: PLoS ONE, Vol 12, Iss 3, p e0161555 (2017)

مصطلحات موضوعية: Medicine, Science

الوصف: BACKGROUND:Environmental factors play a major role on atopic dermatitis (AD) which shows a constant rise in prevalence in western countries over the last decades. The Hygiene Hypothesis suggesting an inverse relationship between incidence of infections and the increase in atopic diseases in these countries, is one of the working hypothesis proposed to explain this trend. OBJECTIVE:This study tested the efficacy and safety of oral administration of the bacterial lysate OM-85 (Broncho-Vaxom®, Broncho-Munal®, Ommunal®, Paxoral®, Vaxoral®), in the treatment of established AD in children. METHODS:Children aged 6 months to 7 years, with confirmed AD diagnosis, were randomized in a double-blind, placebo-controlled trial to receive, in addition to conventional treatment with emollients and topical corticosteroids, 3.5mg of the bacterial extract OM-85 or placebo daily for 9 months. The primary end-point was the difference between groups in the occurrence of new flares (NF) during the study period, evaluated by Hazard Ratio (HR) derived from conditional Cox proportional hazard regression models accounting for repeated events. RESULTS:Among the 179 randomized children, 170 were analysed, 88 in the OM-85 and 82 in the placebo group. As expected most children in both treatment groups experienced at least 1 NF during the study period (75 (85%) patients in the OM-85 group and 72 (88%) in the placebo group). Patients treated with OM-85 as adjuvant therapy had significantly fewer and delayed NFs (HR of repeated flares = 0.80; 95% confidence interval (CI): 0.67-0.96), also when potential confounding factors, as family history of atopy and corticosteroids use, were taken into account (HR = 0.82; 95% CI: 0.69-0.98). No major side effect was reported, with comparable and good tolerability for OM-85 and placebo. CONCLUSIONS:Results show an adjuvant therapeutic effect of a well standardized bacterial lysate OM-85 on established AD.

العلاقة: http://europepmc.org/articles/PMC5363804?pdf=renderTest; https://doaj.org/toc/1932-6203Test; https://doaj.org/article/a1100eb25db84e279461d63589689980Test

الإتاحة: https://doi.org/10.1371/journal.pone.0161555Test
https://doaj.org/article/a1100eb25db84e279461d63589689980Test

المؤلفون: Alain Venet, Martine Sinet, Jean-gerard Guillet, Didier Sicard, Dominique Salmon, Marc Dalod, Broad Intense Anti-human

المساهمون: The Pennsylvania State University CiteSeerX Archives

المصدر: http://jvi.asm.org/content/73/9/7108.full.pdfTest.

الوصف: This article cites 40 articles, 28 of which can be accessed free

وصف الملف: application/pdf

العلاقة: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.620.8483Test; http://jvi.asm.org/content/73/9/7108.full.pdfTest

الإتاحة: http://jvi.asm.org/content/73/9/7108.full.pdfTest

المؤلفون: Pierre Levillain, M. Fournet, Gérard Guillet, P. Roblot, Laurent Misery, Laurent Machet

المصدر: Annales de Dermatologie et de Vénéréologie. 145:564-571

مصطلحات موضوعية: Gynecology, medicine.medical_specialty, business.industry, Paraneoplastic disease, Dermatology, medicine.disease, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, Paraneoplastic pemphigus, 030220 oncology & carcinogenesis, Medicine, Bullous disease, business

الوصف: Resume Contexte Le pemphigus paraneoplasique (PPN) est une maladie tres rare et de mauvais pronostic associant un pemphigus, particulier par certains criteres cliniques, immunologiques et histologiques, a une neoplasie. Methodes Nous avons retrospectivement analyse les dossiers de patients presentant un PPN dans la region Poitou-Charentes entre 2000 et 2015. Resultats Sept patients presentaient neuf neoplasies diagnostiquees de 4 mois avant a 25 mois apres l’apparition des lesions cutanees (6/7) ou muqueuses (6/7) de pemphigus. Les lesions etaient cliniquement polymorphes. L’examen histologique revelait une acantholyse epidermique (7/7), des necroses keratinocytaires (4/7) et une dermite lichenoide d’interface (5/7). On mettait en evidence des depots d’IgG et de C3 intercellulaires ou le long de la jonction dermo-epidermique en immunofluorescence directe (IFD) (7/7), des anticorps anti-substance intercellulaire en immunofluorescence indirecte (IFI) sur vessie de rat (4/6), un immunotransfert reconnaissant les cibles antigeniques du PPN (2/4). La duree du suivi allait de 1 a 132 mois, la survie a 1 an etait de 85,7 %. Discussion Les presentations cliniques et histopathologiques observees chez nos patients sont polymorphes, avec un chevauchement entre les manifestations cliniques et histologiques de PPN et de pemphigus classique. Le pronostic est meilleur dans notre serie que dans la litterature. Les neoplasies associees sont diverses. Il semble exister des associations fortuites de pemphigus a des neoplasies, de meilleur pronostic que les veritables PPN. Un nouveau consensus pour les criteres diagnostiques du PPN est necessaire, qui aiderait les praticiens a diagnostiquer le PPN de maniere plus homogene pour realiser des etudes pronostiques ou des essais therapeutiques.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::da0cb3763cb0a2d10e41c5b527b8d47cTest
https://doi.org/10.1016/j.annder.2018.01.050Test

الوصف: Benjamin se réjouit des vacances de février, mois de carnaval. Comme lui, ses amis et ses cousins préparent la fête et leurs déguisements… La météo perturbe leurs plans. La pluie tombe depuis si longtemps. Elle tombe si fort qu'une inondation extraordinaire noie presque le village. Des animaux viennent se réfugier de partout, envahissent les rues, pillent les jardins, les caves, les boutiques… Un monstre mystérieux sème la panique. Certains croient à une malédiction, le jeune Raphaël, ami de Benjamin, serait le sorcier coupable de cette catastrophe! Sauver Raphaël. Une tâche très difficile pour Benjamin et ses camarades, dans ce drôle de climat…

المؤلفون: Gérard Guillet

نوع المادة: eBook.

تصنيفات: FICTION / General, JUVENILE FICTION / General

الوصف: La cosmétologie prétend s'inspirer de l'harmonie cosmique pour la reproduire sur la peau, à l'image du paon bleu dont les plumes miment avec élégance un ciel étoilé. Son histoire est très riche et, après des siècles, la science s'en est rapprochée pour mener une recherche affinée et l'aider à se renouveler. Le marché est immense avec une multitude de produits d'hygiène, de soin, de parure ou de parfumerie dont la société aurait du mal à se passer. Du choix des ingrédients à la commercialisation du produit fini, cet ouvrage aborde avec une vision critique et constructive toutes ses faces cachées : origine des « actifs », indications, relation avec la dermatologie, enjeux, défis, tests, publicité, tendances, dérives et marché.

المؤلفون: Gérard Guillet

نوع المادة: eBook.

تصنيفات: MEDICAL / General

المؤلفون: Simon Jacobelli, Fedoua Sanaa, Micheline Moyal-Barracco, Monique Pelisse, Sophie Berville, Pascale Villefroy, Marie Odile North, Suzanne Figueiredo, Bénédicte Charmeteau, Thierry Clerici, Françoise Plantier, Françoise Arnold, Antoine Touzé, Nicolas Dupin, Marie-Françoise Avril, Jean-Gérard Guillet, Rémi Cheynier, Isabelle Bourgault-Villada

المصدر: PLoS ONE, Vol 7, Iss 5, p e36651 (2012)

مصطلحات موضوعية: Medicine, Science

الوصف: T-cell responses (proliferation, intracellular cytokine synthesis and IFNγ ELISPOT) against human papillomavirus 16 (HPV16) E2 peptides were tested during 18 months in a longitudinal study in eight women presenting with HPV16-related usual vulvar intraepithelial neoplasia (VIN) and their healthy male partners. In six women, anti-E2 proliferative responses and cytokine production (single IFNγ and/or dual IFNγ/IL2 and/or single IL2) by CD4+ T lymphocytes became detectable after treating and healing of the usual VIN. In the women presenting with persistent lesions despite therapy, no proliferation was observed. Anti-E2 proliferative responses were also observed with dual IFNγ/IL2 production by CD4+ T-cells in six male partners who did not exhibit any genital HPV-related diseases. Ex vivo IFNγ ELISPOT showed numerous effector T-cells producing IFNγ after stimulation by a dominant E2 peptide in all men and women. Since the E2 protein is absent from the viral particles but is required for viral DNA replication, these results suggest a recent infection with replicative HPV16 in male partners. The presence of polyfunctional anti-E2 T-cell responses in the blood of asymptomatic men unambiguously establishes HPV infection even without detectable lesions. These results, despite the small size of the studied group, provide an argument in favor of prophylactic HPV vaccination of young men in order to prevent HPV16 infection and viral transmission from men to women.

العلاقة: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22590583/pdf/?tool=EBITest; https://doaj.org/toc/1932-6203Test; https://doaj.org/article/b1e1d0787b6c43e08ef4199b8c66e3eeTest

الإتاحة: https://doi.org/10.1371/journal.pone.0036651Test
https://doaj.org/article/b1e1d0787b6c43e08ef4199b8c66e3eeTest