المؤلفون: Yuen Zeng, Zichan Guo, Mengqi Wu, Fulin Chen, Lihua Chen

المصدر: Cell Death Discovery, Vol 10, Iss 1, Pp 1-17 (2024)

مصطلحات موضوعية: Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282, Cytology, QH573-671

الوصف: Abstract Circadian rhythms are present in almost all cells and play a crucial role in regulating various biological processes. Maintaining a stable circadian rhythm is essential for overall health. Disruption of this rhythm can alter the expression of clock genes and cancer-related genes, and affect many metabolic pathways and factors, thereby affecting the function of the immune system and contributing to the occurrence and progression of tumors. This paper aims to elucidate the regulatory effects of BMAL1, clock and other clock genes on immune cells, and reveal the molecular mechanism of circadian rhythm’s involvement in tumor and its microenvironment regulation. A deeper understanding of circadian rhythms has the potential to provide new strategies for the treatment of cancer and other immune-related diseases.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2058-7716Test

الوصول الحر: https://doaj.org/article/48dbc677668f4ab48bf5f151ffca7c9dTest

المؤلفون: Mengdi Cheng, Yujie Nie, Min Song, Fulin Chen, Yuan Yu

المصدر: Cell Regeneration, Vol 13, Iss 1, Pp 1-23 (2024)

مصطلحات موضوعية: Stem cell fate, Self-renewal, Quiescence, Differentiation, FOXO, Medicine (General), R5-920, Biology (General), QH301-705.5

الوصف: Abstract Stem cells are pivotal players in the intricate dance of embryonic development, tissue maintenance, and regeneration. Their behavior is delicately balanced between maintaining their pluripotency and differentiating as needed. Disruptions in this balance can lead to a spectrum of diseases, underscoring the importance of unraveling the complex molecular mechanisms that govern stem cell fate. Forkhead box O (FOXO) proteins, a family of transcription factors, are at the heart of this intricate regulation, influencing a myriad of cellular processes such as survival, metabolism, and DNA repair. Their multifaceted role in steering the destiny of stem cells is evident, as they wield influence over self-renewal, quiescence, and lineage-specific differentiation in both embryonic and adult stem cells. This review delves into the structural and regulatory intricacies of FOXO transcription factors, shedding light on their pivotal roles in shaping the fate of stem cells. By providing insights into the specific functions of FOXO in determining stem cell fate, this review aims to pave the way for targeted interventions that could modulate stem cell behavior and potentially revolutionize the treatment and prevention of diseases.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-9769Test

الوصول الحر: https://doaj.org/article/eb0277fa628d4d4fbc2f8a2c5437214aTest

المؤلفون: Yixiong Hu, Fulin Chen, Shikun Zhang, Fuli He, Sihui Ouyang, Fei Peng, Zhenggang Wu, Weidong Zhang

المصدر: Journal of Materials Research and Technology, Vol 29, Iss , Pp 5425-5436 (2024)

مصطلحات موضوعية: Titanium alloy, Cryogenic pre-deformation, Twin, Mechanical properties, Mining engineering. Metallurgy, TN1-997

الوصف: In the present study, pre-deformation at liquid nitrogen temperature (77 K) was applied to introduce twins into Ti–Mo alloys with different contents of molybdenum. The microstructural evolution and mechanical properties with different cryogenic pre-deformation amounts (from 0 to 9 %) were characterized and analyzed. The results show that the twinning activities and the evolution of twinning systems are mainly influenced by β phase stability and cryogenic pre-deformation amount. After cryogenic pre-deformation, the primary and secondary twinning of {332}twins are observed in Ti–12Mo and Ti–15Mo alloys but absent in Ti–20Mo alloy. With the cryogenic pre-deformation amounts increasing, the width and quantity of twins increase constantly. Moreover, {112}twinning is activated in Ti–15Mo alloy pre-deformed by 6 % and 9 %. Twins and high-density dislocations induced by cryogenic pre-deformation cause a significant enhancement in strength of all these three alloys. However, the effect of cryogenic pre-deformation on the elongation of alloys is complicated. For Ti–12Mo alloys, the yield strength increases monotonically (from 527 to 778 MPa) with the increase of cryogenic pre-deformation reduction, while the elongation is completely opposite (from 47 to 20 %). For Ti–15Mo alloys, the optimal matching of strength and ductility is achieved when the amount of cryogenic pre-deformation is 6 %, with a tensile strength of 815 MPa and an elongation of 24 %. Compared with the other two alloys, Ti–20Mo alloy gets a smaller increase in strength and a slight decrease in elongation with the amount of cryogenic pre-deformation increasing.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S223878542400557XTest; https://doaj.org/toc/2238-7854Test

الوصول الحر: https://doaj.org/article/beade25c2262444eac6cf1877926110bTest

المؤلفون: Fulin CHEN

المصدر: Kuangchan zonghe liyong, Vol 45, Iss 1, Pp 139-143 (2024)

مصطلحات موضوعية: mineral processing engineering, low grade, phosphate ore, test on mineral processing, Mining engineering. Metallurgy, TN1-997

الوصف: This is an article in the field of mineral processing engineering. Chemical components, mineral compositions and embedded features of the low grade phosphate had been studied. The main valuable mineral in the ore was collophanite and the main gangue mineral was dolomite. The disseminated extents between valuable mineral and gangue mineral were very small so single reverse flotation method had been used in the article which could make the ore utilize by low lowcost. Through many tests and the beneficiation technological processes of the low grade phosphate had been ascertained that the grinding fineness was 65% below 0.074 mm, and once rougher flotation, twice well-chosen and twice scavenging, and the phosphorus ore concentrate had been gained which yield was 43.97%, P2O5 grade 33.86%, MgO grade 1.35% and the recovery of P2O5 was 81.84% when the raw ore’s P2O5 grade was 18.44% and MgO grade was 9.52%. In this way the cost was low and the low grade phosphate could be utilized effectively.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1000-6532Test

الوصول الحر: https://doaj.org/article/ef1c08a4e6a54c99bd86fefb26bb2289Test

المؤلفون: Pengzhen Cheng, Xueyi Zhao, Meige Han, Yaping Zhuang, Fenru Ning, Yaqian Hu, Weiguang Lu, Sheng Miao, Chengxiang Zhao, Liyuan Jia, Xue Hao, Meng Sun, Junxiang Wang, Fulin Chen, Liu Yang, Qiang Jie

المصدر: Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)

مصطلحات موضوعية: periodic static compression, micro-strain, critical bone defects, endochondral bone formation, biomaterials, Biotechnology, TP248.13-248.65

الوصف: Introduction: Developmental engineering based on endochondral ossification has been proposed as a potential strategy for repairing of critical bone defects. Bone development is driven by growth plate-mediated endochondral ossification. Under physiological conditions, growth plate chondrocytes undergo compressive forces characterized by micro-mechanics, but the regulatory effect of micro-mechanical loading on endochondral bone formation has not been investigated.Methods: In this study, a periodic static compression (PSC) model characterized by micro-strain (with 0.5% strain) was designed to clarify the effects of biochemical/mechanical cues on endochondral bone formation. Hydrogel scaffolds loaded with bone marrow mesenchymal stem cells (BMSCs) were incubated in proliferation medium or chondrogenic medium, and PSC was performed continuously for 14 or 28 days. Subsequently, the scaffold pretreated for 28 days was implanted into rat femoral muscle pouches and femoral condylar defect sites. The chondrogenesis and bone defect repair were evaluated 4 or 10 weeks post-operation.Results: The results showed that PSC stimulation for 14 days significantly increased the number of COL II positive cells in proliferation medium. However, the chondrogenic efficiency of BMSCs was significantly improved in chondrogenic medium, with or without PSC application. The induced chondrocytes (ichondrocytes) spontaneously underwent hypertrophy and maturation, but long-term mechanical stimulation (loading for 28 days) significantly inhibited hypertrophy and mineralization in ichondrocytes. In the heterotopic ossification model, no chondrocytes were found and no significant difference in terms of mineral deposition in each group; However, 4 weeks after implantation into the femoral defect site, all scaffolds that were subjected to biochemical/mechanical cues, either solely or synergistically, showed typical chondrocytes and endochondral bone formation. In addition, simultaneous biochemical induction/mechanical loading significantly accelerated the bone regeneration.Discussion: Our findings suggest that microstrain mechanics, biochemical cues, and in vivo microenvironment synergistically regulate the differentiation fate of BMSCs. Meanwhile, this study shows the potential of micro-strain mechanics in the treatment of critical bone defects.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fbioe.2024.1356135/fullTest; https://doaj.org/toc/2296-4185Test

الوصول الحر: https://doaj.org/article/033b889a11564c54b4bdad7566e40eddTest

المؤلفون: Fulin Chen, Xianyan Cai, Shuo Li, Ning Wu, Zhijie Wang

المصدر: Kuangchan zonghe liyong, Vol 44, Iss 3, Pp 1-6 (2023)

مصطلحات موضوعية: vanadium titanium iron concentrate, mineral characterristics, improving quality, mineral processing engineering, mineralogy, Mining engineering. Metallurgy, TN1-997

الوصف: This is a paper in the field of mineral processing engineering. The mineral characteristics of a vanadium titanium iron concentrate sample from Panxi were studied using sieve analysis, polarizing microscope, X-ray diffractometer, Zeiss Sigma 500 scanning electron microscope, Bruker energy spectrometer, and AMICS automatic mineral analysis system. The main gangue minerals in the sample are magnetite pyrite, granular ilmenite, pyroxene, feldspar, etc. The contents of coarse and fine products TFe, S, SiO2, Al2O3, MgO, and gangue minerals are quite different. A small amount of Fe exists in ilmenite, magnetic pyrite, and non-metallic minerals. Ti mainly exists in the form of titanium magnetite. ilmenite and magnesium aluminum spinel are inlaid on the base of titanium magnetite in lamellar, veinlet, thin strip, grid, and needle shapes, The particle size of the guest crystal mineral is very fine, making it difficult to grind, separate, and post treat the product. The use of grinding magnetic separation can increase the TFe grade of vanadium titanium iron concentrate by 2-3 percentage points. The essence of the improvement is a significant decrease in the content of SiO2, Al2O3, and MgO in the concentrate, with a decrease of SiO2>MgO>Al2O3. The TiO2 content of the concentrate does not change much during the improvement process, with Fe/TiO2 increasing from 4.29 to 4.50, V2O5 grade slightly increasing, and S grade slightly decreasing.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1000-6532Test

الوصول الحر: https://doaj.org/article/7586a6cd8b524286a17cfafb799731aaTest

المؤلفون: Jibin Ma, Zepei Zhang, Jie Lan, Jiwei Tian, Fulin Chen, Jun Miao

المصدر: Frontiers in Surgery, Vol 10 (2023)

مصطلحات موضوعية: tuberculosis, spine, upper thoracic spine, spinal fusion, minimally invasive surgery, Surgery, RD1-811

الوصف: BackgroundThe complex anatomical structure of the upper thoracic spine makes it challenging to achieve surgical exposure, resulting in significant surgical risks and difficulties. Posterior surgery alone fails to adequately address and reconstruct upper thoracic lesions due to limited exposure. While the anterior approach offers advantages in fully exposing the anterior thoracic lesions, the surgical procedure itself is highly intricate. Although there exist various anterior approaches for the upper thoracic spine, the incidence of upper thoracic spine lesions is relatively low. Consequently, there are limited reports on the treatment and reconstruction of upper thoracic spine lesions using the third rib small incision approach in the context of upper thoracic tuberculosis.MethodsWe collected data from four patients with upper thoracic tuberculosis who were admitted to our department between July 2017 and November 2022. The treatment for upper thoracic tuberculosis involved utilizing the third rib small incision approach, which included two cases of thoracic 3–4 vertebral tuberculosis, one case of thoracic 4 vertebral tuberculosis, and one case of thoracic 5 vertebral tuberculosis. Among the patients, three were positioned in the left lateral position, while one was positioned in the right lateral position. Prior to admission, all four patients received a two-week course of oral medication, consisting of isoniazid, rifampicin, pyrazinamide, and ethambutol. After the surgical procedure, they continued receiving anti-tuberculosis treatment for a duration of 12 months.ResultsThe average duration of the surgical procedure was 150 min, with an average blood loss of 500 ml. One patient exhibited symptoms of brachial plexus injury, which gradually improved after careful observation. All patients experienced primary wound healing, and no complications such as pulmonary infection, respiratory failure, or other adverse events were observed. Additionally, one patient showed elevated transaminase levels, leading to a modification in the anti-tuberculosis drug regimen from quadruple therapy to triple therapy.ConclusionThe treatment of upper thoracic tuberculosis through the third rib small incision technique is a very good surgical approach, which has the advantages of safety and effectiveness.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fsurg.2023.1236611/fullTest; https://doaj.org/toc/2296-875XTest

الوصول الحر: https://doaj.org/article/dd24ec17f294455b8f1c693ede3f12b0Test

المؤلفون: Tingting Geng, Miao Li, Rong Chen, Shuangyu Yang, Guoquan Jin, Tinabo Jin, Fulin Chen

المصدر: BMC Cancer, Vol 22, Iss 1, Pp 1-10 (2022)

مصطلحات موضوعية: Lung cancer, Gene polymorphisms, GTF2H1, RAD54L2, MDR analysis, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Abstract Background Repair pathway genes play an important role in the development of lung cancer. The study aimed to assess the correlation between single nucleotide polymorphisms (SNPs) in DNA repair gene (GTF2H1 and RAD54L2) and the risk of lung cancer. Methods Five SNPs in GTF2H1 and four SNPs in RAD54L2 in 506 patients with lung cancer and 510 age-and gender-matched healthy controls were genotyped via the Agena MassARRAY platform. The influence of GTF2H1 and RAD54L2 polymorphisms on lung cancer susceptibility was assessed using logistic regression analysis by calculating odds ratios (ORs) and their corresponding 95% confidence intervals (CIs). Results RAD54L2 rs9864693 GC genotype increased the risk of lung cancer (OR = 1.33, 95%CI: 1.01–1.77, p = 0.045). Stratified analysis found that associations of RAD54L2 rs11720298, RAD54L2 rs4687592, RAD54L2 rs9864693 and GTF2H1 rs4150667 with lung cancer risk were found in subjects aged ≤ 59 years. Precisely, a protective effect of RAD54L2 rs11720298 on the occurrence of lung cancer was observed in non-smokers and drinkers. GTF2H1 rs4150667 was associated with a decreased risk of lung cancer in subjects with BMI ≤ 24 kg/m2. RAD54L2 rs4687592 was associated with an increased risk of lung cancer in drinkers. In addition, GTF2H1 rs3802967 was associated with a reduced risk of lung squamous cell carcinoma. Conclusion Our study first revealed that RAD54L2 rs9864693 was associated with an increased risk of lung cancer in the Chinese Han population. This study may increase the understanding of the effect of RAD54L2 and GTF2H1 polymorphisms on lung cancer occurrence.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2407Test

الوصول الحر: https://doaj.org/article/6c08d0195e3341ddbf76754242d3fb51Test

المؤلفون: Yuying Han, Xiaoliang Gao, Nan Wu, Yirong Jin, He Zhou, Weijie Wang, Hao Liu, Yi Chu, Jiayi Cao, Mingzuo Jiang, Suzhen Yang, Yanting Shi, Xin Xie, Fulin Chen, Ying Han, Wen Qin, Bing Xu, Jie Liang

المصدر: Cell Death and Disease, Vol 13, Iss 8, Pp 1-13 (2022)

مصطلحات موضوعية: Cytology, QH573-671

الوصف: Abstract Ferroptosis, a novel regulated cell death induced by iron-dependent lipid peroxidation, plays an important role in tumor development and drug resistance. Long noncoding RNAs (lncRNAs) are associated with various types of cancer. However, the precise roles of many lncRNAs in tumorigenesis remain elusive. Here we explored the transcriptomic profiles of lncRNAs in primary CRC tissues and corresponding paired adjacent non-tumor tissues by RNA-seq and found that LINC00239 was significantly overexpressed in colorectal cancer tissues. Abnormally high expression of LINC00239 predicts poorer survival and prognosis in colorectal cancer patients. Concurrently, we elucidated the role of LINC00239 as a tumor-promoting factor in CRC through in vitro functional studies and in vivo tumor xenograft models. Importantly, overexpression of LINC00239 decreased the anti-tumor activity of erastin and RSL3 by inhibiting ferroptosis. Collectively, these data suggest that LINC00239 plays a novel and indispensable role in ferroptosis by nucleotides 1–315 of LINC00239 to interact with the Kelch domain (Nrf2-binding site) of Keap1, inhibiting Nrf2 ubiquitination and increasing Nrf2 protein stability. Considering the recurrence and chemoresistance constitute the leading cause of death in colorectal cancer (CRC), ferroptosis induction may be a promising therapeutic strategy for CRC patients with low LINC00239 expression.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2041-4889Test

الوصول الحر: https://doaj.org/article/2b8756e63065444d981331ee3bddd4ceTest

المؤلفون: Xin Leng, Mengyao She, Xilang Jin, Jiao Chen, Xuehao Ma, Fulin Chen, Jianli Li, Bingqin Yang

المصدر: Frontiers in Nutrition, Vol 9 (2022)

مصطلحات موضوعية: trace metal, fluorescent probe, copper ion, test strips, cells imaging, Nutrition. Foods and food supply, TX341-641

الوصف: Copper is a vital trace metal in human body, which plays the significant roles in amounts of physiological and pathological processes. The application of copper-selective probe has attracted great interests from environmental tests to life process research, yet a few of sensitive Cu2+ tests based on on-site analysis have been reported. In this paper, a novel fluorescein-based fluorescent probe N4 was designed, synthesized, and characterized, which exhibited high selectivity and sensitivity to Cu2+ comparing with other metal ions in ethanol–water (1/1, v/v) solution. The probe N4 bonded with Cu2+ to facilitate the ring-opening, and an obvious new band at 525 nm in the fluorescence spectroscopy appeared, which could be used for naked-eye detection of Cu2+ within a broad pH range of 6–9. Meanwhile, a good linearity between the fluorescence intensity and the concentrations of Cu2+ ranged 0.1–1.5 eq. was observed, and the limit of detection of N4 to Cu2+ was calculated to be as low as 1.20 μm. In addition, the interaction mode between N4 and Cu2+ was found to be 1:1 by the Job's plot and mass experiment. Biological experiments showed that the probe N4 exhibited low biological toxicity and could be applied for Cu2+ imaging in living cells. The significant color shift associated with the production of the N4-Cu2+ complex at low micromolar concentrations under UV light endows N4 with a promising probe for field testing of trace Cu2+ ions.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fnut.2022.932826/fullTest; https://doaj.org/toc/2296-861XTest

الوصول الحر: https://doaj.org/article/6705e054fefd455590601d2d05406ad4Test