المؤلفون: Blanco Federico Carlos, Vazquez Cristina Lourdes, García Julia Sabio y, Rocha Rosana Valeria, Gravisaco María José, Forrellad Marina Andrea, Magistrelli Giovanni, Bigi Fabiana

المصدر: Journal of Veterinary Research, Vol 65, Iss 3, Pp 315-321 (2021)

مصطلحات موضوعية: bovine gm-csf, bovine il-4, dendritic cells, macrophages, mammalian episomal expression, Veterinary medicine, SF600-1100

الوصف: Granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) are cytokines widely used in ex vivo monocyte differentiation experiments, vaccine formulations and disease treatment. The aim of this study was to produce recombinant bovine GM-CSF and IL-4 in an episomal expression system that conserves the postransductional modification of the native proteins and to use the products to differentiate bovine monocytes into dendritic cells.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2450-8608Test

الوصول الحر: https://doaj.org/article/239419e86afa4b7bb6a838e9e1f988e6Test

المؤلفون: Villafañe, Luciana, Rocha, Rosana Valeria, Bigi, María Mercedes, Klepp, Laura Inés, Taboga, Oscar Alberto, Forrellad, Marina Andrea, López, María Gabriela, Bigi, Fabiana

المصدر: Vet Immunol Immunopathol ; ISSN:1873-2534 ; Volume:273

مصطلحات موضوعية: Antigens, EsxG, IFN-γ assay, Mb0309, Mycobacterium bovis

الوصف: Bovine tuberculosis (bTB) represents a threat to livestock production. Mycobacterium bovis is the main causative agent of bTB and a pathogen capable of infecting wildlife and humans. Eradication programs based on surveillance in slaughterhouses with mandatory testing and culling of reactive cattle have failed to eradicate bTB in many regions worldwide. Therefore, developing effective tools to control this disease is crucial. Using a computational tool, we identified proteins in the M. bovis proteome that carry predictive binding peptides to BoLADRB3.2 and selected Mb0309, Mb1090, Mb1810 and Mb3810 from all the identified proteins. The expression of these proteins in a baculovirus-insect cell expression system was successful only for Mb0309 and Mb3810. In parallel, we expressed the ESAT-6 family proteins EsxG and EsxH in this system. Among the recombinant proteins, Mb0309 and EsxG exhibited moderate performance in distinguishing between cattle that test positive and negative to bTB using the official test, the intradermal tuberculin test (IDT), when used to stimulate interferon-gamma production in blood samples from cattle. However, when combined as a protein cocktail, Mb0309 and EsxG were reactive in 50 % of positive cattle. Further assessments in cattle that evade the IDT (false negative) and cattle infected with Mycobacterium avium paratuberculosis are necessary to determine the potential utility of this cocktail as an additional tool to assist the accurate diagnosis of bTB.

العلاقة: https://doi.org/10.1016/j.vetimm.2024.110788Test; https://pubmed.ncbi.nlm.nih.gov/38838485Test

الإتاحة: https://doi.org/10.1016/j.vetimm.2024.110788Test
https://pubmed.ncbi.nlm.nih.gov/38838485Test

المؤلفون: Forrellad, Marina Andrea, Blanco, Federico Carlos, Marrero Diaz de Villegas, Rubén, Vázquez, Cristina Lourdes, Yaneff, Agustín, García, Elizabeth Andrea, Gutierrez, Maximiliano Gabriel, Durán, Rosario, Villarino, Andrea, Bigi, Fabiana

المصدر: Frontiers in Microbiology ; volume 11 ; ISSN 1664-302X

مصطلحات موضوعية: Microbiology (medical), Microbiology

الإتاحة: https://doi.org/10.3389/fmicb.2020.570794Test

المؤلفون: Soriano Pérez, Maria Laura, Funes, Javier Alejandro, Flores Bracamonte, Carolina, Ibarra, Luis Exequiel, Forrellad, Marina Andrea, Taboga, Oscar, Cariddi, Laura Noelia, Salinas, Facundo José, Ortega, Hugo Héctor, Alustiza, Fabrisio, Molina, Maria

المصدر: International Journal of Pharmaceutics ; volume 630, page 122435 ; ISSN 0378-5173

مصطلحات موضوعية: Pharmaceutical Science

الإتاحة: https://doi.org/10.1016/j.ijpharm.2022.122435Test
https://api.elsevier.com/content/article/PII:S0378517322009905?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0378517322009905?httpAccept=text/plainTest

المؤلفون: Forrellad, Marina Andrea, Vazquez, Cristina Lourdes, Blanco, Federico Carlos, Klepp, Laura Ines, Garcia, Elizabeth Andrea, Rocha, Rosana Valeria, Villafañe, Luciana Maria, Bigi, María Mercedes, Gutierrez, Maximiliano Gabriel, Bigi, Fabiana

المصدر: Virulence 10 (1) : 1026-1033. (Diciembre 2019)

مصطلحات موضوعية: Mycobacterium Tuberculosis, Estrés Oxidativo, Virulencia, Patogenicidad, Oxidative Stress, Virulence, Pathogenicity, Rv2617c, Erp

الوصف: In this study, we characterized the role of Rv2617c in the virulence of Mycobacterium tuberculosis. Rv2617c is a protein of unknown function unique to M. tuberculosis complex (MTC) and Mycobacterium leprae. In vitro, this protein interacts with the virulence factor P36 (also named Erp) and KdpF, a protein linked to nitrosative stress. Here, we showed that knockout of the Rv2617c gene in M. tuberculosis CDC1551 reduced the replication of the pathogen in a mouse model of infection and favored the trafficking of mycobacteria to phagolysosomes. We also demonstrated that Rv2617c and P36 are required for resistance to in vitro hydrogen peroxide treatment in M. tuberculosis and Mycobacterium bovis, respectively. These findings indicate Rv2617c and P36 act in concert to prevent bacterial damage upon oxidative stress. ; Instituto de Biotecnología ; Fil: Forrellad, Marina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Klepp, Laura Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Rocha, Rosana Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Villafañe, Luciana ...

وصف الملف: application/pdf

العلاقة: info:eu-repograntAgreement/INTA/PNBIO/1131034/AR./Inmunología molecular y genómica funcional aplicadas a interacciones patógeno hospedador de interés pecuario.; http://hdl.handle.net/20.500.12123/6682Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930017Test/; 2150-5608 (Online); https://doi.org/10.1080/21505594.2019.1693714Test

الإتاحة: https://doi.org/20.500.12123/6682Test
https://doi.org/10.1080/21505594.2019.1693714Test
https://hdl.handle.net/20.500.12123/6682Test
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930017Test/

المؤلفون: Vazquez, Cristina Lourdes, Bianco, María Veronica, Blanco, Federico Carlos, Forrellad, Marina Andrea, Gutierrez, Maximiliano Gabriel, Bigi, Fabiana

المصدر: Infection and immunity 85 (3): e00720-16. (2017 Mar.)

مصطلحات موضوعية: Mycobacterium Bovis, Enfermedades de los Animales, Tuberculosis, Macrofagos, Ganado Bovino, Animal Diseases, Macrophages, Cattle, LprG

الوصف: Mycobacterium bovis causes tuberculosis in a wide variety of mammals, with strong tropism for cattle and eventually humans. P27, also called LprG, is among the proteins involved in the mechanisms of the virulence and persistence of M. bovis and Mycobacterium tuberculosis. Here, we describe a novel function of P27 in the interaction of M. bovis with its natural host cell, the bovine macrophage. We found that a deletion in the p27-p55 operon impairs the replication of M. bovis in bovine macrophages. Importantly, we show for the first time that M. bovis arrests phagosome maturation in a process that depends on P27. This effect is P27 specific since complementation with wild-type p27 but not p55 fully restored the wild-type phenotype of the mutant strain; this indicates that P55 plays no important role during the early events of M. bovis infection. In addition, we also showed that the presence of P27 from M. smegmatis decreases the association of LAMP-3 with bead phagosomes, indicating that P27 itself blocks phagosome-lysosome fusion by modulating the traffic machinery in the cell host. ; Instituto de Biotecnología ; Fil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Forrellad, Marina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Gutierrez, Maximiliano Gabriel. The Francis Crick Institute, Host-Pathogen Interactions in Tuberculosis Laboratory; Reino Unido ; Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina

وصف الملف: application/pdf

العلاقة: http://hdl.handle.net/20.500.12123/552Test; http://iai.asm.org/content/85/3/e00720-16.full.pdf+htmlTest; https://doi.org/10.1128/IAI.00720-16Test

الإتاحة: https://doi.org/20.500.12123/552Test
https://doi.org/10.1128/IAI.00720-16Test
https://hdl.handle.net/20.500.12123/552Test
http://iai.asm.org/content/85/3/e00720-16.full.pdf+htmlTest

المؤلفون: Forrellad, Marina Andrea, Klepp, Laura Ines, Gioffre, Andrea, Sabio y Garcia, Julia Veronica, Morbidoni, Hector R., Santangelo, María De La Paz, Cataldi, Angel Adrian, Bigi, Fabiana

المصدر: Virulence 4 (1) : 3-66 (2013)

مصطلحات موضوعية: Mycobacterium tuberculosis, Virulencia, Patogenicidad, Control de Enfermedades, Vacuna, Virulence, Pathogenicity, Disease Control, Vaccines

الوصف: The Mycobacterium tuberculosis complex (MTBC) consists of closely related species that cause tuberculosis in both humans and animals. This illness, still today, remains to be one of the leading causes of morbidity and mortality throughout the world. The mycobacteria enter the host by air, and, once in the lungs, are phagocytated by macrophages. This may lead to the rapid elimination of the bacillus or to the triggering of an active tuberculosis infection. A large number of different virulence factors have evolved in MTBC members as a response to the host immune reaction. The aim of this review is to describe the bacterial genes/proteins that are essential for the virulence of MTBC species, and that have been demonstrated in an in vivo model of infection. Knowledge of MTBC virulence factors is essential for the development of new vaccines and drugs to help manage the disease toward an increasingly more tuberculosis-free world. ; Instituto de Biotecnología ; Fil: Forrellad, Marina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Klepp, Laura Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Gioffre, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Sabio y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Morbidoni, Hector R. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Cátedra de Microbiología; Argentina ; Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. ; Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina

وصف الملف: application/pdf

العلاقة: http://hdl.handle.net/20.500.12123/4293Test; https://doi.org/10.4161/viru.22329Test

الإتاحة: https://doi.org/20.500.12123/4293Test
https://doi.org/10.4161/viru.22329Test
https://hdl.handle.net/20.500.12123/4293Test

المؤلفون: Forrellad, Marina Andrea, Bianco, María Veronica, Blanco, Federico Carlos, Nuñez, Javier, Klepp, Laura Ines, Vazquez, Cristina Lourdes, Santangelo, María De La Paz, Rocha, Rosana Valeria, Soria, Marcelo, Golby, Paul, Gutierrez, Maximiliano Gabriel, Bigi, Fabiana

المصدر: BMC Microbiology 13 : 200 (2013)

مصطلحات موضوعية: Mycobacterium tuberculosis, Enfermedades Humanas, Tuberculosis, Experimentación en Laboratorio, Proteínas, Human Diseases, Laboratory Experimentation, Proteins

الوصف: Background: Tuberculosis is one of the leading causes of mortality throughout the world. Mycobacterium tuberculosis, the agent of human tuberculosis, has developed strategies involving proteins and other compounds called virulence factors to subvert human host defences and damage and invade the human host. Among these virulence-related proteins are the Mce proteins, which are encoded in the mce1, mce2, mce3 and mce4 operons of M. tuberculosis. The expression of the mce2 operon is negatively regulated by the Mce2R transcriptional repressor. Here we evaluated the role of Mce2R during the infection of M. tuberculosis in mice and macrophages and defined the genes whose expression is in vitro regulated by this transcriptional repressor. Results: We used a specialized transduction method for generating a mce2R mutant of M. tuberculosis H37Rv. Although we found equivalent replication of the MtΔmce2R mutant and the wild type strains in mouse lungs, overexpression of Mce2R in the complemented strain (MtΔmce2RComp) significantly impaired its replication. During in vitro infection of macrophages, we observed a significantly increased association of the late endosomal marker LAMP-2 to MtΔmce2RComp-containing phagosomes as compared to MtΔmce2R and the wild type strains. Whole transcriptional analysis showed that Mce2R regulates mainly the expression of the mce2 operon, in the in vitro conditions studied. Conclusions; The findings of the current study indicate that Mce2R weakly represses the in vivo expression of the mce2 operon in the studied conditions and argue for a role of the proteins encoded in Mce2R regulon in the arrest of phagosome maturation induced by M. tuberculosis. ; Instituto de Biotecnología ; Fil: Forrellad, Marina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología ...

وصف الملف: application/pdf

العلاقة: https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-13-200Test; http://hdl.handle.net/20.500.12123/4265Test; https://doi.org/10.1186/1471-2180-13-200Test

الإتاحة: https://doi.org/20.500.12123/4265Test
https://doi.org/10.1186/1471-2180-13-200Test
https://hdl.handle.net/20.500.12123/4265Test

المؤلفون: Bianco, María Verónica, Blanco, Federico Carlos, Forrellad, Marina Andrea, Aguilar, Diana, Campos, Eleonora, Klepp, Laura Inés, Hernández-Pando, Rogelio, Cataldi, Angel Adrian, Bigi, Fabiana

المصدر: Virulence ; volume 2, issue 3, page 233-237 ; ISSN 2150-5594 2150-5608

الإتاحة: https://doi.org/10.4161/viru.2.3.15888Test

المؤلفون: Villafañe, Luciana, Forrellad, Marina Andrea, López, María Gabriela, Garbaccio, Sergio, Garro, Carlos, Rocha, Rosana Valeria, Eirin, María Emilia, Singh, Mahavir, Taboga, Oscar A., Bigi, Fabiana

المصدر: Microbial Physiology ; volume 29, issue 1-6, page 83-90 ; ISSN 2673-1665 2673-1673

مصطلحات موضوعية: Cell Biology, Applied Microbiology and Biotechnology, Physiology, Biochemistry, Microbiology, Biotechnology

الوصف: Bovine tuberculosis (bTB) is a disease produced by Mycobacterium bovis that affects livestock, wild animals, and humans. The classical diagnostic method to detect bTB is measuring the response induced with the intradermal injection of purified protein derivative of M. bovis (PPDb). Another ancillary bTB test detects IFN-γ produced in whole blood upon stimulation with PPDb, protein/peptide cocktails, or individual antigens. Among the most used M. bovis antigens in IFN-γ assays are the secreted proteins ESAT-6 and CFP-10, which together with antigen Rv3615c improve the sensitivity of the test in comparison to PPDb. Protein reagents for immune stimulation are generally obtained from Escherichia coli , because this bacterium produces a high level of recombinant proteins. However, E. coli recombinant antigens are in general contaminated with lipopolysaccharides and other components that produce non-specific IFN-γ secretion in in vitro assays. In this work, we produced the relevant ESAT-6, CFP-10, and Rv3615c M. bovis antigens as fusions to the polyhedrin protein from the baculovirus AcMNPV. We obtained chimeric proteins effectively incorporated to the occlusion bodies and easily purified the recombinant polyhedra with no reactive contaminants. In an IFN-γ assay, these fusion proteins showed equivalent sensibility but better specificity than the same M. bovis proteins produced in E. coli .

الإتاحة: https://doi.org/10.1159/000506687Test
https://www.karger.com/Article/Pdf/506687Test