المؤلفون: Kalkanci Ayse, Dizbay Murat, Sari Nuran, Yalcin Burce, Fidan Isil, Arman Dilek, Kustimur Semra

المصدر: Open Medicine, Vol 5, Iss 2, Pp 194-197 (2010)

مصطلحات موضوعية: antifungals, chequerboard, e-test, Medicine

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2391-5463Test

الوصول الحر: https://doaj.org/article/f7644296069f46d9a42a82560decf7dcTest

المؤلفون: GÜZEL TUNÇCAN, ÖZLEM, KALKANCI, AYŞE, Salimi, Duygu Deniz Usta, Escan, Funda, FİDAN, IŞIL, YAR SAĞLAM, ATİYE SEDA

الوصف: There are limited number of studies investigating the role of microRNAs (miRNAs) in Aspergillus infections. In this study, we designed an in vitro aspergillosis model to identify differentially expressed Aspergillus-related miRNAs. For this purpose, carcinoma cell lines “A549” and “Calu-3” were infected with Aspergillus fumigatus. Total miRNA was isolated at 0, 1, 6, and 24 h post-infection. Quantitative real-time PCR assay was conducted to screen 31 human miRNAs that were possibly related to aspergillosis. Up- and downregulated miRNAs were detected in the infected cells. Highest level of miRNA expression was detected at 6 h post-infection. miR-21, hsa-miR-186-5p, hsa-miR-490-5p, miR-26a-5p, miR-26b-5p, hsa-miR-424-5p, hsa-miR-548d-3p, hsa-miR-196a-5p, miR-150-5p, miR-17-5p, and hsa-miR-99b-5p were found to be significantly upregulated (p < 0.001) at 6 h after A. fumigatus infection compared with the controls. Among the screened miRNAs, hsa-miR-145-5p (p < 0.001); hsa-miR-583 and hsa-miR-3978 (p < 0.01); and miR-21-5p, hsa-miR-4488, and hsa-miR-4454 (p < 0.05) were found to be downregulated compared with the controls. In conclusion, screening the identified miRNAs may reveal the personal predisposition to aspergillosis, which might be valuable from the perspective of personalized medicine.

العلاقة: eb60dfc7-d5a6-4779-98e8-09aa4c65c594; https://avesis.gazi.edu.tr/publication/details/eb60dfc7-d5a6-4779-98e8-09aa4c65c594/oaiTest

الإتاحة: https://doi.org/10.1007/s13353-024-00871-2Test
https://avesis.gazi.edu.tr/publication/details/eb60dfc7-d5a6-4779-98e8-09aa4c65c594/oaiTest

المؤلفون: Fidan, IŞIL, Bozdayi, GÜLENDAM, Tug, ESRA, Akdogan, Dogan, Lale, Zubeyde, Tunccan, ÖZLEM, Yildirim, Fatma

الوصف: Background. The viral spike (S) protein and host ACE2 and TMPRSS2 genetic variations may act as a barrier to viral infections or determine susceptibility to severe acute respiratory syndrome coronavirus 2 (SARSCoV-2) infections.Objectives. We investigated the relationship between the expression patterns and polymorphisms of the ACE2 and TMPRSS2 receptor genes associated with coronavirus disease 2019 (COVID-19) and the clinical course of SARS-CoV-2 infections.Materials and methods. We examined 147 COVID-19 patients (41 asymptomatic, 53 symptomatic and 53 cases treated in the intensive care unit (ICU)) and 33 healthy controls. The ACE2 and TMPRSS2 expression was determined using the One-Run RT-qPCR kit. Genotypic distributions of single nucleotide polymorphisms (SNPs) of ACE2 and TMPRSS2 were obtained using reverse transcription quantitative polymerase chain reaction (RT-qPCR).Results. The expressions of ACE2 and TMPRSS2 were different between SARS-CoV-2-positive and-negative groups. The ACE2 rs714205GG genotype and G-allele showed significant differences in the asymptomatic SARSCoV-2-positive group. A significant correlation was found between the expression of TMPRSS2 rs8134378GA, rs2070788GA, rs7364083GA, and rs9974589AC genotypes and SARS-CoV-2 positivity. The rs1978124 C allele and rs8134378 A-allele expressions were significant in the symptomatic SARS-CoV-2-positive group. The TMPRSS2 rs2070788GA expression was different in all patient groups compared to the control group. There was a difference between SARS-CoV-2-positive and-negative groups regarding the CTTA haplotype formed by ACE2 variants. The AGCAG and AGAAG haplotypes formed by the TMPRSS2 variants were more common in the asymptomatic patient group than in other patient groups.Conclusions. Identifying the relationship between host genetic variants and COVID-19 susceptibility will contribute to further studies, enabling new vaccines and potential therapeutic approaches to be discovered.

العلاقة: 160bdf95-573f-4a4d-9441-fd94be2304d7; https://avesis.gazi.edu.tr/publication/details/160bdf95-573f-4a4d-9441-fd94be2304d7/oaiTest

الإتاحة: https://doi.org/10.17219/acem/163409Test
https://avesis.gazi.edu.tr/publication/details/160bdf95-573f-4a4d-9441-fd94be2304d7/oaiTest

المؤلفون: Ulucakoy, Rezan Kocak, FİDAN, IŞIL, GÜNENDİ, ZAFER, GÖĞÜŞ, FERİDE NUR, BOZDAYI, GÜLENDAM, BATIBAY, SEVİLAY

الوصف: Objectives To determine the seroconversion (SC) rate after CoronaVac and BNT162b2 vaccines in adults with inflammatory rheumatic disease (IRD). Methods Patients who were followed up with IRD and who received two doses of either CoronaVac or BNT162b2 vaccines were included in this prospective observational single-center study. Subjects with two doses of CoronaVac or BNT162b2 without known IRD were included in the healthy controls. The blood samples were taken at a minimum of two and a maximum of 12 weeks after the second dose of vaccine. Results A total of 81 patients with IRD (61 CoronaVac, 20 BNT162b2) and 100 healthy controls (70 CoronaVac, 30 BNT162b2) were included. The SC rate was slightly lower among patients with IRD versus controls (84 vs 97%, p = 0.002). The SC rate was 100% in all participants who received BNT162b2 both in the patient and control group. The IgG antibody level after CoronaVac in the patient group was significantly lower than both the BNT162b2 (p = 0.031) and the healthy group (p < 0.001). Among patients with IRD, those on rituximab (RTX) (12/81,14.8%) had significantly less SC rate (5/12, 41.7%). The median neutralizing antibody titers were significantly higher in patients with BNT162b2 compared with CoronaVac (1.97 vs. 16.34, p < 0.001). Conclusions This study showed that all patients with BNT162b2 vaccine developed immunogenicity in patients with IRD, while there was a decreased antibody response with CoronaVac vaccine compared to that of BNT162b2. In particular, RTX significantly reduces the SC rate.

العلاقة: 1923e0c0-c7c2-4ad7-836a-027782e2aa72; https://avesis.gazi.edu.tr/publication/details/1923e0c0-c7c2-4ad7-836a-027782e2aa72/oaiTest

الإتاحة: https://doi.org/10.1007/s10787-022-01089-6Test
https://avesis.gazi.edu.tr/publication/details/1923e0c0-c7c2-4ad7-836a-027782e2aa72/oaiTest

المؤلفون: Tug, Esra, Fidan, Isil, Bozdayi, Gulendam, Yildirim, Fatma, Tunccan, Ozlem Guzel, Lale, Zubeyde, Akdogan, Dogan

المصدر: Advances in Clinical & Experimental Medicine; Jan2024, Vol. 33 Issue 1, p39-51, 13p

مصطلحات موضوعية: SARS-CoV-2, GENE expression, ANGIOTENSIN converting enzyme, REVERSE transcriptase polymerase chain reaction, CORONAVIRUS diseases, COVID-19

مستخلص: Background. The viral spike (S) protein and host ACE2 and TMPRSS2 genetic variations may act as a barrier to viral infections or determine susceptibility to severe acute respiratory syndrome coronavirus 2 (SARSCoV-2) infections. Objectives. We investigated the relationship between the expression patterns and polymorphisms of the ACE2 and TMPRSS2 receptor genes associated with coronavirus disease 2019 (COVID-19) and the clinical course of SARS-CoV-2 infections. Materials and methods. We examined 147 COVID-19 patients (41 asymptomatic, 53 symptomatic and 53 cases treated in the intensive care unit (ICU)) and 33 healthy controls. The ACE2 and TMPRSS2 expression was determined using the One-Run RT-qPCR kit. Genotypic distributions of single nucleotide polymorphisms (SNPs) of ACE2 and TMPRSS2 were obtained using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Results. The expressions of ACE2 and TMPRSS2 were different between SARS-CoV-2-positive and -negative groups. The ACE2 rs714205GG genotype and G-allele showed significant differences in the asymptomatic SARSCoV-2-positive group. A significant correlation was found between the expression of TMPRSS2 rs8134378GA, rs2070788GA, rs7364083GA, and rs9974589AC genotypes and SARS-CoV-2 positivity. The rs1978124 Callele and rs8134378 A-allele expressions were significant in the symptomatic SARS-CoV-2-positive group. The TMPRSS2 rs2070788GA expression was different in all patient groups compared to the control group. There was a difference between SARS-CoV-2-positive and -negative groups regarding the CTTA haplotype formed by ACE2 variants. The AGCAG and AGAAG haplotypes formed by the TMPRSS2 variants were more common in the asymptomatic patient group than in other patient groups. Conclusions. Identifying the relationship between host genetic variants and COVID-19 susceptibility will contribute to further studies, enabling new vaccines and potential therapeutic approaches to be discovered. [ABSTRACT FROM AUTHOR]

: Copyright of Advances in Clinical & Experimental Medicine is the property of Wroclaw Medical University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Kuralay, Zeynep Koc, Fidan, Işıl, Tuğ, Esra

الوصف: Some single nucleotide polymorphisms (SNPs) of the gene encoding interleukin 28B (IL28B) may increase susceptibility to infection and chronicity in humans with hepatitis B and C viruses. In our study, we aimed to investigate the prevalence of rs12979860, rs8099917 and rs12980275 SNPs in IL28B in patients with hepatitis B (HBV) or hepatitis C Virus (HCV) infection and to determine the relationship of these polymorphisms with plasma IL28B levels. For this purpose, 64 HBV-infected and 66 HCV-infected patients and 70 healthy individuals were included in the study. The SNPs were investigated by real time PCR (Polimerase Chain Reaction, Rt-PCR) using TaqMan SNP Genotyping Assay. The plasma levels of IL28B were detected by 'Enzyme Linked Immunosorbent Assay (ELISA)'. The frequencies of the rs12980275AG genotype and G allele (p= 0.003 and p= 0.04, respectively), and the rs12979860CT genotype and T allele (p= 0.01 and p= 0.04, respectively) were lower in HBV-infected patients. In HCV-infected patients, the rs8099917TG genotype and G allele frequencies (p= 0.04) were higher and the TGG haplotype showed a statistically significant difference (p= 0.04). The mean of IL28B plasma levels were higher in the control group than the HBV or HCV-infected patient groups (p= 0.001 and p= 0.01, respectively). However, HBV-infected patients with the rs12980275AG genotype showed a significant difference in plasma IL28B levels compared to the other genotypes (p= 0.0001) and these patients had lower viral loads (<105 IU/ml). According to the results of the study, it can be stated that rs12979860CT and rs12980275AG genotypes may play a role in preventing the chronicity of HBV infection, while rs8099917TG genotype may contribute to the transformation of HCV infection into chronic infection. In this study, it was observed that the presence of the G allele for the rs8099917 polymorphism could be evaluated as a risk allele for chronic HCV infection and that the TGG haplotype could have a strong predictive effect on increasing susceptibility ...

العلاقة: 09a12598-7552-4dfa-a914-0b86e1239544; https://avesis.gazi.edu.tr/publication/details/09a12598-7552-4dfa-a914-0b86e1239544/oaiTest

الإتاحة: https://doi.org/10.5578/mb.20219807Test
https://avesis.gazi.edu.tr/publication/details/09a12598-7552-4dfa-a914-0b86e1239544/oaiTest

المؤلفون: Sahin, Erdem, Bozdayi, Gulendam, Yigit, Selin, Muftah, Hager, Dizbay, Murat, Tunccan, Ozlem G., Fidan, Isil, Caglar, Kayhan

المصدر: Journal of Medical Virology ; volume 93, issue 10, page 6016-6026 ; ISSN 0146-6615 1096-9071

الوصف: Novel mutations have been emerging in the genome of severe acute respiratory syndrome‐coronavirus 2 (SARS‐CoV‐2); consequently, the evolving of more virulent and treatment resistance strains have the potential to increase transmissibility and mortality rates. The characterization of full‐length SARS‐CoV‐2 genomes is critical for understanding the origin and transmission pathways of the virus, as well as identifying mutations that affect the transmissibility and pathogenicity of the virus. We present an analysis of the mutation pattern and clade distribution of full‐length SARS‐CoV‐2 genome sequences obtained from specimens tested at Gazi University Medical Virology Laboratory. Viral RNA was extracted from nasopharyngeal specimens. Next‐generation sequencing libraries were prepared and sequenced on Illumina iSeq 100 platform. Raw sequencing data were processed to obtain full‐length genome sequences and variant calling was performed to analyze amino acid changes. Clade distribution was determined to understand the phylogenetic background in relation to global data. A total of 293 distinct mutations were identified, of which 152 missense, 124 synonymous, 12 noncoding, and 5 deletions. The most frequent mutations were P323L (nsp12), D614G (ORF2/S), and 2421C>T (5′‐untranslated region) found simultaneously in all sequences. Novel mutations were found in nsp12 (V111A, H133R, Y453C, M626K) and ORF2/S (R995G, V1068L). Nine different Pangolin lineages were detected. The most frequently assigned lineage was B.1.1 (17 sequences), followed by B.1 (7 sequences) and B.1.1.36 (3 sequences). Sequence information is essential for revealing genomic diversity. Mutations might have significant functional implications and analysis of these mutations provides valuable information for therapeutic and vaccine development studies. Our findings point to the introduction of the virus into Turkey through various sources and the subsequent spread of several key variants.

الإتاحة: https://doi.org/10.1002/jmv.27188Test

المؤلفون: Bozdayi, Gulendam, Yildirim, Fatma, Fidan, Isil, Tug, Esra, Akdogan, Dogan, Tunccan, ÖZLEM, Lale, Zubeyde

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=od_____10046::1528de9c6c810df772dbe9d7d9d6122aTest
https://avesis.gazi.edu.tr/publication/details/160bdf95-573f-4a4d-9441-fd94be2304d7/oaiTest

المؤلفون: Dalgıç, Buket, Ekinci, Özgür, Sarı, Sinan, Polat, Meltem, Tezer, Hasan, Tapısız, Anıl, Teker Düztaş, Demet, Fidan, Işıl, Eğritaş Gürkan, Ödül, Bedir Demirdağ, Tuğba

العلاقة: f3d427d5-9864-4a88-bf08-b9844b763fd6; https://avesis.gazi.edu.tr/publication/details/f3d427d5-9864-4a88-bf08-b9844b763fd6/oaiTest

الإتاحة: https://avesis.gazi.edu.tr/publication/details/f3d427d5-9864-4a88-bf08-b9844b763fd6/oaiTest

المؤلفون: Fidan, Işıl

العلاقة: e90adbc8-0b4b-4805-adb1-caaec10e95a3; https://avesis.gazi.edu.tr/publication/details/e90adbc8-0b4b-4805-adb1-caaec10e95a3/oaiTest

الإتاحة: https://avesis.gazi.edu.tr/publication/details/e90adbc8-0b4b-4805-adb1-caaec10e95a3/oaiTest