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1رسالة جامعية
المؤلفون: Zamani, Neviaty Putri
مصطلحات موضوعية: 590, Bleaching, Coral, Feulgen staining
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2دورية أكاديمية
المؤلفون: Noushin Jalayer naderi
المصدر: Iranian Journal of Pathology, Vol 13, Iss 1, Pp 106-107 (2018)
مصطلحات موضوعية: feulgen staining, staining technique, nucleus, nuclear abnormalities, Pathology, RB1-214
الوصف: Among different staining methods used to demonstrate the nuclear abnormalities, Feulgen is one of the most reliable method. Feulgen staining is specific, sensitive method for evaluating the DNA damages.It has been shown that using non-DNA specific stains for monitoring the nuclear anomalies lead to false-positive or false-negative results. From self-experience, immersing the stained slides in hematoxylin for 3-5 minutes in final step after staining the slides with light green, provides a greater ground contrast and very better visualizing of nucleus. By this method, a clear distinction can be made between micronuclei and other structures suggestive of a micronucleus.
العلاقة: https://ijp.iranpath.org/article_30054_a4ea3c6c5d2095028edcc80c84fd8475.pdfTest; https://doaj.org/toc/1735-5303Test; https://doaj.org/toc/2345-3656Test; https://doaj.org/article/85838772c85446e0bbfd56c584ebf30fTest
الإتاحة: https://doi.org/10.30699/ijp.13.1.106Test
https://doaj.org/article/85838772c85446e0bbfd56c584ebf30fTest -
3دورية أكاديمية
المؤلفون: BENJAMIN RODRIGUEZ GARAY
المساهمون: ALEJANDRA GUILLERMINA GONZALEZ GUTIERREZ
المصدر: SpringerPlus (2016) 5:1804
مصطلحات موضوعية: info:eu-repo/classification/Autor/Monosporic, Polygonum-type, Central cell, Helobial endosperm, Postament, Hypostase, Zygotic embryo, Confocal microscopy, Feulgen staining, info:eu-repo/classification/cti/6
الوصف: The genus Polianthes belongs to the subfamily Agavoideae of the Asparagaceae family formerly known as Agavaceae. The genus is endemic to México and comprises about 15 species, among them is Polianthes tuberosa L. The aim of this work was to study and characterize the embryo sac and early embryo development of this species in order to generate basic knowledge for its use in taxonomy, in vitro fertilization and production of haploid plants and to complement studies already performed in other genera and species belonging to the Agavoideae sub-family. It was found that the normal development of the P. tuberosa var. Simple embryo sac follows a monosporic pattern of the Polygonum type and starts its development from the chalazal megaspore. At maturity, the embryo sac is of a pyriform shape with a chalazal haustorial tube where the antipodals are located, just below the hypostase, which connects the embryo sac with the nucellar tissue of the ovule. The central cell nucleus shows a high polarity, being located at the chalazal extreme of the embryo sac. The position of cells inside the P. tuberosa embryo sac may be useful for in depth studies about the double fertilization. Furthermore, it was possible to make a chronological description of the events that happen from fertilization and early embryo development to the initial development of the endosperm which was classified as of the helobial type.
وصف الملف: application/pdf
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4دورية أكاديميةFocusing on genera to improve species identification: Revised systematics of the ciliate Spirostomum
المؤلفون: BOSCARO, VITTORIO, D. Carducci, G. Barbieri, M. Senra, I. Andreoli, F. Erra, PETRONI, GIULIO, VERNI, FRANCO, FOKIN, SERGEY
المساهمون: Boscaro, Vittorio, D., Carducci, G., Barbieri, M., Senra, I., Andreoli, F., Erra, Petroni, Giulio, Verni, Franco, Fokin, Sergey
مصطلحات موضوعية: Feulgen staining, Heterotrichea, ITS, macronucleus type, SSU rRNA, 18S rRNA
الوصف: Although many papers dealing with the description of new ciliate taxa are published each year, species taxonomy and identification in most groups of the phylum Ciliophora remain confused. This is largely due to a scarcity of surveys on the systematics of immediately higher levels (genera and families) providing data for old and new species together. Spirostomum is a common and distinctive inhabitant of fresh- and brackish water environments, including artificial and eutrophic ones, and is a good model for applied ecology and symbiosis research. Despite this, only 3 of the numerous species are commonly cited, and no studies have yet confirmed their monophyly, with the consequence that reproducibility of the results may be flawed. In this paper we present morphological and molecular data for 30 Spirostomum populations representing 6 different morphospecies, some of which were collected in previously unreported countries. We performed a detailed revision of Spirostomum systematics combining literature surveys, new data on hundreds of organisms and statistical and phylogenetic analyses; our results provide insights on the evolution, ecology and distribution of known morphospecies and a novel one: Spirostomum subtilis sp. n. We also offer tools for quick species identification. © 2014 Elsevier GmbH.
وصف الملف: STAMPA
العلاقة: info:eu-repo/semantics/altIdentifier/wos/WOS:000340583300009; volume:165; issue:4; firstpage:527; lastpage:541; numberofpages:15; journal:PROTIST; http://hdl.handle.net/11568/683066Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84903766464; https://www.sciencedirect.com/science/article/pii/S1434461014000510Test
الإتاحة: https://doi.org/10.1016/j.protis.2014.05.004Test
http://hdl.handle.net/11568/683066Test
https://www.sciencedirect.com/science/article/pii/S1434461014000510Test -
5
المؤلفون: Zhao Yang Chen, Kelly Y.P. Liu, Sarah Yuqi Zhu, Martial Guillaud, Alan Harrison, Catherine F. Poh
المصدر: PLoS ONE, Vol 16, Iss 11, p e0259529 (2021)
PLoS ONE
PLoS ONE, Vol 16, Iss 11 (2021)مصطلحات موضوعية: Male, Oncology, Epidemiology, medicine.medical_treatment, H&E stain, Metastasis, Machine Learning, Medicine and Health Sciences, Group-Specific Staining, Cutoff, Staining, Multidisciplinary, Cancer Risk Factors, Middle Aged, Primary tumor, Carcinoma, Squamous Cell, Biomarker (medicine), Medicine, Female, Anatomy, Research Article, medicine.medical_specialty, Science, Surgical and Invasive Medical Procedures, Research and Analysis Methods, Lymphatic System, Malignant Tumors, Text mining, Internal medicine, medicine, Humans, Differentiated Tumors, Aged, Cell Nucleus, Squamous Cell Carcinoma of Head and Neck, business.industry, Hematoxylin Staining, Cancers and Neoplasms, Biology and Life Sciences, Neck dissection, medicine.disease, Specimen Preparation and Treatment, Medical Risk Factors, Cancer cell, Lymph Nodes, business, Feulgen Staining
الوصف: Background Early-stage oral squamous cell carcinoma (OSCC) patients have a one-in-four risk of regional metastasis (LN+), which is also the most significant prognostic factor for survival. As there are no validated biomarkers for predicting LN+ in early-stage OSCC, elective neck dissection often leads to over-treatment and under-treatment. We present a machine-learning-based model using the quantitative nuclear phenotype of cancer cells from the primary tumor to predict the risk of nodal disease. Methods and findings Tumor specimens were obtained from 35 patients diagnosed with primary OSCC and received surgery with curative intent. Of the 35 patients, 29 had well (G1) or moderately (G2) differentiated tumors, and six had poorly differentiated tumors. From each, two consecutive sections were stained for hematoxylin & eosin and Feulgen-thionin staining. The slides were scanned, and images were processed to curate nuclear morphometric features for each nucleus, measuring nuclear morphology, DNA amount, and chromatin texture/organization. The nuclei (n = 384,041) from 15 G1 and 14 G2 tumors were randomly split into 80% training and 20% test set to build the predictive model by using Random Forest (RF) analysis which give each tumor cell a score, NRS. The area under ROC curve (AUC) was 99.6% and 90.7% for the training and test sets, respectively. At the cutoff score of 0.5 as the median NRS of each region of interest (n = 481), the AUC was 95.1%. We then developed a patient-level model based on the percentage of cells with an NRS ≥ 0.5. The prediction performance showed AUC of 97.7% among the 80% (n = 23 patient) training set and with the cutoff of 61% positive cells achieved 100% sensitivity and 91.7% specificity. When applying the 61% cutoff to the 20% test set patients, the model achieved 100% accuracy. Conclusions Our findings may have a clinical impact with an easy, accurate, and objective biomarker from routine pathology tissue, providing an unprecedented opportunity to improve neck management decisions in early-stage OSCC patients.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::38f80d1785e7d353016e0598319820a9Test
https://doaj.org/article/f1acb1bb69d64b41813b4b138b971940Test -
6Focusing on Genera to Improve Species Identification: Revised Systematics of the Ciliate Spirostomum
المؤلفون: Vittorio Boscaro, Daniela Carducci, Ilaria Andreoli, Giulio Petroni, Marcus Vinicius Xavier Senra, Sergei I. Fokin, Fabrizio Erra, Franco Verni, Giovanna Barbieri
المصدر: Protist. 165:527-541
مصطلحات موضوعية: Systematics, biology, Phylogenetic tree, Ecology, Applied ecology, Spirostomum, biology.organism_classification, Microbiology, Heterotrichea, 18S ribosomal RNA, 18S rRNA, Monophyly, Taxon, Feulgen staining, macronucleus type, RNA, Ribosomal, 18S, Taxonomy (biology), ITS, Ciliophora, Feulgen staining, Heterotrichea, ITS, macronucleus type, SSU rRNA, 18S rRNA, SSU rRNA, Phylogeny
الوصف: Although many papers dealing with the description of new ciliate taxa are published each year, species taxonomy and identification in most groups of the phylum Ciliophora remain confused. This is largely due to a scarcity of surveys on the systematics of immediately higher levels (genera and families) providing data for old and new species together. Spirostomum is a common and distinctive inhabitant of fresh- and brackish water environments, including artificial and eutrophic ones, and is a good model for applied ecology and symbiosis research. Despite this, only 3 of the numerous species are commonly cited, and no studies have yet confirmed their monophyly, with the consequence that reproducibility of the results may be flawed. In this paper we present morphological and molecular data for 30 Spirostomum populations representing 6 different morphospecies, some of which were collected in previously unreported countries. We performed a detailed revision of Spirostomum systematics combining literature surveys, new data on hundreds of organisms and statistical and phylogenetic analyses; our results provide insights on the evolution, ecology and distribution of known morphospecies and a novel one: Spirostomum subtilis sp. n. We also offer tools for quick species identification.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::603dfe4b6b9c38dbb0a5148c54baef43Test
https://doi.org/10.1016/j.protis.2014.05.004Test -
7دورية أكاديمية
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8
المصدر: SpringerPlus
مصطلحات موضوعية: Confocal microscopy, Polygonum-type, animal structures, Research, Feulgen staining, embryonic structures, Hypostase, Postament, Zygotic embryo, Monosporic, Central cell, Helobial endosperm
الوصف: The genus Polianthes belongs to the subfamily Agavoideae of the Asparagaceae family formerly known as Agavaceae. The genus is endemic to México and comprises about 15 species, among them is Polianthes tuberosa L. The aim of this work was to study and characterize the embryo sac and early embryo development of this species in order to generate basic knowledge for its use in taxonomy, in vitro fertilization and production of haploid plants and to complement studies already performed in other genera and species belonging to the Agavoideae sub-family. It was found that the normal development of the P. tuberosa var. Simple embryo sac follows a monosporic pattern of the Polygonum type and starts its development from the chalazal megaspore. At maturity, the embryo sac is of a pyriform shape with a chalazal haustorial tube where the antipodals are located, just below the hypostase, which connects the embryo sac with the nucellar tissue of the ovule. The central cell nucleus shows a high polarity, being located at the chalazal extreme of the embryo sac. The position of cells inside the P. tuberosa embryo sac may be useful for in depth studies about the double fertilization. Furthermore, it was possible to make a chronological description of the events that happen from fertilization and early embryo development to the initial development of the endosperm which was classified as of the helobial type.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::86bbd9285d74a28ad32b298ac3de970bTest
https://pubmed.ncbi.nlm.nih.gov/27812444Test -
9
المؤلفون: Gabriel Landini, Gionata De Vico, Francesca Carella
المساهمون: Carella, Francesca, DE VICO, Gionata, Landini, Gabriel
المصدر: PLoS ONE, Vol 12, Iss 3, p e0173219 (2017)
PLoS ONEمصطلحات موضوعية: 0301 basic medicine, Pathology, B Cells, Hemocytes, lcsh:Medicine, Severity of Illness Index, White Blood Cells, Spectrum Analysis Techniques, Ploidy, Animal Cells, Neoplasms, Medicine and Health Sciences, Mussels, lcsh:Science, Staining, education.field_of_study, Multidisciplinary, biology, Mytilus, medicine.anatomical_structure, Spectrophotometry, Cellular Types, Anatomy, Algorithms, Research Article, Bivalves, medicine.medical_specialty, Histology, Imaging Techniques, Immune Cells, Immunology, Population, Research and Analysis Methods, 03 medical and health sciences, Multinucleate, Genetics, medicine, Animals, Antibody-Producing Cells, education, Cell Nucleus, Evolutionary Biology, Blood Cells, Ploidies, Population Biology, Morphometry, lcsh:R, Organisms, Biology and Life Sciences, Cell Biology, Molluscs, Mussel, biology.organism_classification, Bivalvia, Invertebrates, Cell nucleus, 030104 developmental biology, Specimen Preparation and Treatment, lcsh:Q, Densitometry, Population Genetics, Feulgen Staining
الوصف: Haemic neoplasia (HN) in bivalves has been reported in association with mass mortality events in various species of molluscs. The aim of this work was to quantify the nuclear morphometry and DNA content of neoplastic cells of mussels Mytilus galloprovincialis affected by HN using nuclear densitometry in Feulgen-stained preparations. The results were also compared with a population of normal mussel haemocytes. We captured 256 images of 3 different neoplasia stages and 120 images of normal haemocytes; thus, a total of 120,166 nuclei were analysed. We extracted 21 morphological parameters from normal and neoplastic nuclei. Eighteen of these parameters were different (P
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b2d6b582e19d8bab018572e12281302Test
https://doi.org/10.1371/journal.pone.0173219Test -
10
المؤلفون: DD Mandana, Selvamani, GS Madhushankari, SB Praveen, Mohsin Gadiwan, DS Pradeep
المصدر: Journal of Oral and Maxillofacial Pathology : JOMFP
مصطلحات موضوعية: Epithelial dysplasia, Pathology, medicine.medical_specialty, nuclear morphometry, Nuclear area, Context (language use), Biological potential, Biology, medicine.disease, Pathology and Forensic Medicine, Otorhinolaryngology, Dysplasia, oral epithelial dysplasia, Feulgen staining, medicine, Original Article, Feulgen stain, General Dentistry, Moderate Dysplasia, Leukoplakia
الوصف: Context: Abnormalities in nuclear morphology are very frequently seen in dysplasia, such as nuclear area, diameter, shape, number of nucleoli and membrane outline. The purpose of this study was to observe and compare the nuclear features in different grades of epithelial dysplasia in leukoplakia and to evaluate the use of Feulgen stain for observing the nuclear features in oral epithelial dysplasia in leukoplakia. Seventy paraffin embedded tissue section (20 mild, 20 moderate, 20 severe dysplasia cases and 10 control specimens) were analyzed for nuclear morphology using Feulgen stain under trinocular research microscope. Statistically significant results were obtained with P > 0.001, when intergroup comparison was done except in case of nuclear area and diameter between mild and moderate dysplasia. Nuclear features reflect cell's biological potential and its morphometry was found to be a useful tool for differentiating different grades of dysplasia.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a0fd035588b314cb1c3f53e7d06bfdeTest
https://pubmed.ncbi.nlm.nih.gov/25328298Test
النص الكامل