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المؤلفون: Luiz Gonzaga Tone, Rosane Gomes de Paula Queiroz, Pablo Ferreira das Chagas, Pamela Viani de Andrade, Paulo Henrique dos Santos Klinger, Elvis Terci Valera, Régia Caroline Peixoto Lira, Felipe Amstalden Trevisan, Carlos Alberto Scrideli, Ricardo Santos de Oliveira, Lara Elis Alberici Delsin, Augusto Faria Andrade, Gustavo Alencastro Veiga Cruzeiro
المصدر: Scientific Reports, Vol 10, Iss 1, Pp 1-6 (2020)
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Scientific Reportsمصطلحات موضوعية: Programmed cell death, Radiation-Sensitizing Agents, lcsh:Medicine, Apoptosis, FARMACOLOGIA, Article, chemistry.chemical_compound, Arsenic Trioxide, Cell Line, Tumor, Humans, Viability assay, Arsenic trioxide, Clonogenic assay, Cytotoxicity, Child, lcsh:Science, Multidisciplinary, Dose-Response Relationship, Drug, lcsh:R, Cell cycle, Neoplasm Proteins, CNS cancer, chemistry, Cell culture, Cancer research, lcsh:Q, Drug Screening Assays, Antitumor, Medulloblastoma
الوصف: We evaluated the potential effects of ATO in different pediatric SHH-MB cell lines (ONS-76: TP53-wild type; DAOY and UW402: TP53-mutated). MB cell lines molecular subgroup was confirmed and TP53 mutations were validated. Cell viability, clonogenicity and apoptosis were evaluated after ATO treatment at different concentrations (1–16 µM) alone or combined with irradiation doses (0.5, 1, 2 and 4 Gy). Rad51 and Ku86 proteins were evaluated by WB. ATO treatment reduced cell viability for all SHH-MB cell lines. Significant decrease of clonogenic capacity and higher apoptosis rates were also observed after ATO exposure, being cell death more pronounced (>70%) for the SHH-MB TP53-mutated. Combined treatment of ATO with irradiation also reduced colonies formation in UW402 tumor cells, which was independent of DNA damage repair proteins Rad51 and Ku86. In silico analyses suggested that a set of genes from cell cycle and p53 pathways are differentially expressed in SHH tumor subtypes, suggesting that cell lines may respond to therapies according to the gene expression profiles. Herein, we showed ATO cytotoxicity in pediatric SHH cell lines, with marked radiosensitizing effect for the MB-SHH TP53-mutated cells. These results highlight the potential of ATO, alone or in combination with radiotherapy, supporting further clinical investigations.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0bedda3250e22daa120da0a65af09586Test
http://link.springer.com/article/10.1038/s41598-020-63808-9Test -
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المؤلفون: Andressa Romualdo Rodrigues, Fernanda Maris Peria, Felipe Amstalden Trevisan, Daniela Pretti da Cunha Tirapelli, Carlos Gilberto Carlotti Júnior, Fermino Sanches Lizarte Neto, Mucio Luiz de Assis Cirino
المصدر: Rep Pract Oncol Radiother
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USPمصطلحات موضوعية: Temozolomide, business.industry, medicine.medical_treatment, Cancer, APOPTOSE, medicine.disease, 030218 nuclear medicine & medical imaging, Radiation therapy, 03 medical and health sciences, 0302 clinical medicine, Real-time polymerase chain reaction, Oncology, Apoptosis, 030220 oncology & carcinogenesis, microRNA, Gene expression, Cancer research, Medicine, Radiology, Nuclear Medicine and imaging, Viability assay, Original Research Article, business, medicine.drug
الوصف: Aim To evaluate the effect of radiotherapy and temozolomide on the expression of miRNAs apoptotic (miRNAs-21, -221, -222 (anti-apoptotic) and miRNAs-15a, -16 (pro-apoptotic)) and the gene MGMT in glioblastoma cell lines. Background The limited knowledge of the molecular biology of malignant gliomas may hinder the development of therapeutic modalities. In this scenario, one of the greatest advances of recent years was the identification of microRNAs. These molecules have an important role in biological processes involving cancer, including glioblastoma. Materials and methods Trypan blue was used to verify the cell viability, and real time PCR to quantify the expression of microRNAs and gene 24, 48 and 120 h after exposure to treatments. Results There was a statistically significant decrease of expression of miR-15a between 48 and 120 h in line T98 G treated with radiation, increased expression of miR-15a between 24 and 120 h in line U251 treated with radiation and temozolomide, and increased expression of miR-16 between 24 and 120 h in line U251 treated with radiation alone and when combined with temozolomide. There was a decrease in MGMT gene expression, between 24 and 48 h in U343 cells treated with temozolomide. Conclusions Ionizing radiation and temozolomide modified the expression of miRNAs studied and MGMT.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d146d20ea28bfb6e0912dc66bd63b797Test
https://pubmed.ncbi.nlm.nih.gov/32684859Test -
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المؤلفون: Gabriela Pereira-da-Silva, Caio M. Matias, Carlos Gilberto Carlotti, Fernanda Maris Peria, Daniela Pretti da Cunha Tirapelli, Felipe Amstalden Trevisan, Mucio Luiz de Assis Cirino, Andressa Romualdo Rodrigues, Fermino Sanches Lizarte Neto
المصدر: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USPمصطلحات موضوعية: 0301 basic medicine, Male, Methyltransferase, medicine.medical_treatment, Population, RADIAÇÃO IONIZANTE, 03 medical and health sciences, 0302 clinical medicine, Cancer stem cell, Radiation, Ionizing, microRNA, medicine, Biomarkers, Tumor, Temozolomide, Humans, education, Molecular Biology, DNA Modification Methylases, Aged, Aged, 80 and over, Chemotherapy, education.field_of_study, business.industry, Brain Neoplasms, General Neuroscience, Tumor Suppressor Proteins, DNA Methylation, Middle Aged, Prognosis, Radiation therapy, MicroRNAs, 030104 developmental biology, DNA Repair Enzymes, DNA methylation, Cancer research, Neoplastic Stem Cells, Female, Neurology (clinical), business, Glioblastoma, 030217 neurology & neurosurgery, Brazil, Developmental Biology, medicine.drug
الوصف: Despite the increased understanding of the oncological mechanisms underlying Glioblastoma multiforme (GBM) pathophysiology, and recent advances in therapeutic strategies such as maximal surgical resection and post-operative radiotherapy with concomitant and adjuvant temozolomide chemotherapy, the prognosis for patients with brain tumors remains limited. Evidences indicate that the assessment of DNA methylation status in cancer stem cells would allow identifying molecules expressed in these cells, to lead to targeted elimination of this critical population from brain tumors, making the glioblastoma treatment more effective. This study aimed to analyze the role of microRNA-181d associated with the methylation status of the O6-methylguanine methyl transferase (MGMT) gene in Glioblastoma multiforme cancer stem cells subjected to treatment with temozolomide and ionizing radiation. Such responses were analyzed in terms of cell survival, evaluation of the MGMT gene methylation status by MS-HRM (Methylation-Sensitive High Resolution Melting), and analysis of miRNA-181d and MGMT gene expression by relative quantification of mRNA levels in cancer stem cells subjected to treatment with temozolomide and ionizing radiation, isolated or combined. We showed that ionizing radiation and temozolomide reduced the viability of cancer stem cells from GBM patients, as well as modified MGMT gene and miRNA-181d expression in cancer stem cells, suggesting that miRNA-181d interferes in the glioblastoma cancer stem cell response to treatment with temozolomide and ionizing radiation.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8115620fb4ee9b72eacf9d55def32ec2Test
https://pubmed.ncbi.nlm.nih.gov/31226325Test -
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المؤلفون: D.P.C. Tirapelli, G. Pereira-da-Silva, M.F.G.S. Tazima, Fernanda Maris Peria, Mucio Luiz de Assis Cirino, Breno Nery, Daniel Guimarães Tiezzi, Fermino Sanzes Lizarte Neto, L.F. Tirapelli, Felipe Amstalden Trevisan, L.G. Lourenço, Andressa Romualdo Rodrigues, C.G. Carlotti Junior
المصدر: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USPمصطلحات موضوعية: Temozolomide, Glioblastoma cell line, RADIAÇÃO IONIZANTE, General Medicine, Biology, Ionizing radiation, Neurosphere, microRNA, Genetics, medicine, Cancer research, Research article, Molecular Biology, After treatment, medicine.drug
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1fcf29c3faaeb8b071c21207fa255cbfTest
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المؤلفون: Michele Ceccarelli, Floris P. Barthel, Tathiane M. Malta, Thais S. Sabedot, Sofie R. Salama, Bradley A. Murray, Olena Morozova, Yulia Newton, Amie Radenbaugh, Stefano M. Pagnotta, Samreen Anjum, Jiguang Wang, Ganiraju Manyam, Pietro Zoppoli, Shiyun Ling, Arjun A. Rao, Mia Grifford, Andrew D. Cherniack, Hailei Zhang, Laila Poisson, Carlos Gilberto Carlotti, Daniela Pretti da Cunha Tirapelli, Arvind Rao, Tom Mikkelsen, Ching C. Lau, W.K. Alfred Yung, Raul Rabadan, Jason Huse, Daniel J. Brat, Norman L. Lehman, Jill S. Barnholtz-Sloan, Siyuan Zheng, Kenneth Hess, Ganesh Rao, Matthew Meyerson, Rameen Beroukhim, Lee Cooper, Rehan Akbani, Margaret Wrensch, David Haussler, Kenneth D. Aldape, Peter W. Laird, David H. Gutmann, Houtan Noushmehr, Antonio Iavarone, Roel G.W. Verhaak, Harindra Arachchi, J. Todd Auman, Miruna Balasundaram, Saianand Balu, Gene Barnett, Stephen Baylin, Sue Bell, Christopher Benz, Natalie Bir, Keith L. Black, Tom Bodenheimer, Lori Boice, Moiz S. Bootwalla, Jay Bowen, Christopher A. Bristow, Yaron S.N. Butterfield, Qing-Rong Chen, Lynda Chin, Juok Cho, Eric Chuah, Sudha Chudamani, Simon G. Coetzee, Mark L. Cohen, Howard Colman, Marta Couce, Fulvio D’Angelo, Tanja Davidsen, Amy Davis, John A. Demchok, Karen Devine, Li Ding, Rebecca Duell, J. Bradley Elder, Jennifer M. Eschbacher, Ashley Fehrenbach, Martin Ferguson, Scott Frazer, Gregory Fuller, Jordonna Fulop, Stacey B. Gabriel, Luciano Garofano, Julie M. Gastier-Foster, Nils Gehlenborg, Mark Gerken, Gad Getz, Caterina Giannini, William J. Gibson, Angela Hadjipanayis, D. Neil Hayes, David I. Heiman, Beth Hermes, Joe Hilty, Katherine A. Hoadley, Alan P. Hoyle, Mei Huang, Stuart R. Jefferys, Corbin D. Jones, Steven J.M. Jones, Zhenlin Ju, Alison Kastl, Ady Kendler, Jaegil Kim, Raju Kucherlapati, Phillip H. Lai, Michael S. Lawrence, Semin Lee, Kristen M. Leraas, Tara M. Lichtenberg, Pei Lin, Yuexin Liu, Jia Liu, Julia Y. Ljubimova, Yiling Lu, Yussanne Ma, Dennis T. Maglinte, Harshad S. Mahadeshwar, Marco A. Marra, Mary McGraw, Christopher McPherson, Shaowu Meng, Piotr A. Mieczkowski, C. Ryan Miller, Gordon B. Mills, Richard A. Moore, Lisle E. Mose, Andrew J. Mungall, Rashi Naresh, Theresa Naska, Luciano Neder, Michael S. Noble, Ardene Noss, Brian Patrick O’Neill, Quinn T. Ostrom, Cheryl Palmer, Angeliki Pantazi, Michael Parfenov, Peter J. Park, Joel S. Parker, Charles M. Perou, Christopher R. Pierson, Todd Pihl, Alexei Protopopov, Nilsa C. Ramirez, W. Kimryn Rathmell, Xiaojia Ren, Jeffrey Roach, A. Gordon Robertson, Gordon Saksena, Jacqueline E. Schein, Steven E. Schumacher, Jonathan Seidman, Kelly Senecal, Sahil Seth, Hui Shen, Yan Shi, Juliann Shih, Kristen Shimmel, Hugues Sicotte, Suzanne Sifri, Tiago Silva, Janae V. Simons, Rosy Singh, Tara Skelly, Andrew E. Sloan, Heidi J. Sofia, Matthew G. Soloway, Xingzhi Song, Carrie Sougnez, Camila Souza, Susan M. Staugaitis, Huandong Sun, Charlie Sun, Donghui Tan, Jiabin Tang, Yufang Tang, Leigh Thorne, Felipe Amstalden Trevisan, Timothy Triche, David J. Van Den Berg, Umadevi Veluvolu, Doug Voet, Yunhu Wan, Zhining Wang, Ronald Warnick, John N. Weinstein, Daniel J. Weisenberger, Matthew D. Wilkerson, Felicia Williams, Lisa Wise, Yingli Wolinsky, Junyuan Wu, Andrew W. Xu, Lixing Yang, Liming Yang, Travis I. Zack, Jean C. Zenklusen, Jianhua Zhang, Wei Zhang, Jiashan Zhang, Erik Zmuda
المساهمون: Ceccarelli, M, Barthel, Fp, Malta, Tm, Sabedot, T, Salama, Sr, Murray, Ba, Morozova, O, Newton, Y, Radenbaugh, A, Pagnotta, Sm, Anjum, S, Wang, Jg, Manyam, G, Zoppoli, P, Ling, S, Rao, Aa, Grifford, M, Cherniack, Ad, Zhang, Hl, Poisson, L, Carlotti, Cg, Tirapelli, Dpd, Rao, A, Mikkelsen, T, Lau, Cc, Yung, Wka, Rabadan, R, Huse, J, Brat, Dj, Lehman, Nl, Barnholtz-Sloan, J, Zheng, S, Hess, K, Rao, G, Meyerson, M, Beroukhim, R, Cooper, L, Akbani, R, Wrensch, M, Haussler, D, Aldape, Kd, Laird, Pw, Gutmann, Dh, Noushmehr, H, Iavarone, A, Verhaak, Rgw, Neurosurgery
المصدر: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Ceccarelli, M, Barthel, F P, Malta, T M, Sabedot, T S, Salama, S R, Murray, B A, Morozova, O, Newton, Y, Radenbaugh, A, Pagnotta, S M, Anjum, S, Wang, J, Manyam, G, Zoppoli, P, Ling, S, Rao, A A, Grifford, M, Cherniack, A D, Zhang, H, Poisson, L, Carlotti, C G J, Tirapelli, D P D C, Rao, A, Mikkelsen, T, Lau, C C, Yung, W K A, Rabadan, R, Huse, J, Brat, D J, Lehman, N L, Barnholtz-Sloan, J S, Zheng, S, Hess, K, Rao, G, Meyerson, M, Beroukhim, R, Cooper, L, Akbani, R, Wrensch, M, Haussler, D, Aldape, K D, Laird, P W, Gutmann, D H, Noushmehr, H, Iavarone, A & Verhaak, R G W 2016, ' Molecular Profiling Reveals Biologically Discrete Subsets and Pathways of Progression in Diffuse Glioma ', Cell, vol. 164, no. 3, pp. 550-563 . https://doi.org/10.1016/j.cell.2015.12.028Test
Cell, 164(3), 550-563. Cell Pressمصطلحات موضوعية: 0301 basic medicine, Adult, X-linked Nuclear Protein, Biology, General Biochemistry, Genetics and Molecular Biology, Article, Epigenesis, Genetic, Transcriptome, 03 medical and health sciences, Diffuse Glioma, Glioma, medicine, Cluster Analysis, Humans, Promoter Regions, Genetic, Gene, Telomerase, ATRX, Cell Proliferation, Pilocytic astrocytoma, Biochemistry, Genetics and Molecular Biology(all), Brain Neoplasms, MUTAÇÃO GENÉTICA, DNA Helicases, Nuclear Proteins, DNA Methylation, Middle Aged, Telomere, medicine.disease, Isocitrate Dehydrogenase, 3. Good health, 030104 developmental biology, DNA demethylation, DNA methylation, Mutation, Cancer research, Signal Transduction
الوصف: Therapy development for adult diffuse glioma is hindered by incomplete knowledge of somatic glioma driving alterations and suboptimal disease classification. We defined the complete set of genes associated with 1,122 diffuse grade II-III-IV gliomas from The Cancer Genome Atlas and used molecular profiles to improve disease classification, identify molecular correlations, and provide insights into the progression from low- to high-grade disease. Whole-genome sequencing data analysis determined that ATRX but not TERT promoter mutations are associated with increased telomere length. Recent advances in glioma classification based on IDH mutation and 1p/19q co-deletion status were recapitulated through analysis of DNA methylation profiles, which identified clinically relevant molecular subsets. A subtype of IDH mutant glioma was associated with DNA demethylation and poor outcome; a group of IDH-wild-type diffuse glioma showed molecular similarity to pilocytic astrocytoma and relatively favorable survival. Understanding of cohesive disease groups may aid improved clinical outcomes.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::632ac454203f4f8d554b72918f8683a4Test
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المؤلفون: Piotr A. Mieczkowski, Jianhua Zhang, Saianand Balu, Marc Ladanyi, Victor E. Reuter, Johanna Gardner, Junyuan Wu, Troy Shelton, Simone Chevalier, Marco A. Marra, Anuradha Gopalan, Roy Tarnuzzer, Pei Lin, Joel S. Parker, Umadevi Veluvolu, Lisle E. Mose, Rebecca Carlsen, Michel Carmel, Shalini S. Yadav, Joseph Paulauskis, Fadi Brimo, Sheila Reynolds, Scott Frazer, Natalya Dyakova, Christopher A. Bristow, Brian D. Robinson, Lucie Hamel, Yaron S.N. Butterfield, Yao Fu, Syed Haider, Christopher J. Logothetis, Michael Parfenov, Jeff Boyd, Katherine Tarvin, Kjong-Van Lehmann, Stuart R. Jefferys, Alan P. Hoyle, Arshi Arora, A. Gordon Robertson, Matti Annala, Thomas L. Bauer, Jianhua Luo, Rodolfo Borges Dos Reis, Louis Lacombe, Andrew Salner, Matti Nykter, Alexandre Doueik, Mei Huang, Alireza Moinzadeh, Joshua Armenia, Andre Kahles, Rehan Akbani, Todd Pihl, Gordon Saksena, Robert A. Holt, Felipe Amstalden Trevisan, Mario Berrios, Nina Thiessen, Ronglai Shen, Carrie Sougnez, Xiaojia Ren, Matthew G. Soloway, Lixing Yang, W. Marston Linehan, Chip Stewart, Angeliki Pantazi, Alain Bergeron, Andrea Sboner, Angela Hadjipanayis, Xingzhi Song, Carlos Gilberto Carlotti, Manaswi Gupta, Rashi Naresh, Yiling Lu, Hartmut Juhl, Mark Gerstein, Robert Leung, Sahil Seth, Teri A. Longacre, Ignaty Leshchiner, Chris Sander, Andrew Wei Xu, Anne Marie Mes-Masson, Patrick Teebagy, Julian M. Hess, Matthew Meyerson, Adrian Ally, Jiabin Tang, Ye Wu, Janae V. Simons, David Van Den Berg, Kenna R. Mills Shaw, Patricia Troncoso, Thomas Gribbin, Shannon J. McCall, Andrew K. Godwin, Daniel J. Weisenberger, Kimberly Rieger-Christ, Nilsa C. Ramirez, Peter J. Park, Margi Sheth, Mark E. Sherman, Eric S. Lander, Yunhu Wan, Lisa Iype, Robert Penny, J. Todd Auman, Ekta Khurana, Stephen J. Freedland, Jeffry Simko, Nils Weinhold, Bradley M. Broom, Angela Tam, Nikolaus Schultz, Erik Zmuda, Wei Zhang, Wenyi Wang, Jeffrey Roach, Christopher E. Barbieri, Alan H. Bryce, Qingguo Wang, Lawrence D. True, Christopher C. Benz, Mathieu Latour, Richard A. Moore, Michael Mayo, Yi Zhong, Tara M. Lichtenberg, Jacqueline E. Schein, Svitlana Tyekucheva, Ranabir Guin, Scott L. Carter, Michael Kerger, David Canes, Scott M. Lippman, Alexei Protopopov, Katayoon Kasaian, Peter A. Pinto, Peter S. Nelson, W. Kimryn Rathmell, David Mallery, Brett S. Carver, David I. Heiman, Juok Cho, Doug Voet, Thorsten Schlomm, Janet E. Cowan, Heidi J. Sofia, Peggy Yena, Matthew D. Wilkerson, Glenn Bubley, Tucker W. LeBien, Yan Shi, Lucas Lochovsky, Gordon B. Mills, Hailei Zhang, Andrea Holbrook, Christopher M. Hovens, Christina Yau, Jonathan G. Seidman, Samirkumar B. Amin, Corbin D. Jones, Andrew D. Cherniack, Lori Boice, Laxmi Lolla, Kristen M. Leraas, Denise Brooks, Francesca Demichelis, Maria Christina Tsourlakis, Katherine A. Hoadley, Charles Saller, Leigh B. Thorne, Julie M. Gastier-Foster, Jaegil Kim, Rameen Beroukhim, Peter R. Carroll, John A. Demchok, Christopher J. Kane, Jay Bowen, Massimo Loda, Richard Cartun, Ina Felau, Carl Morrison, Kerstin David, Eliezer M. Van Allen, Laura S. Schmidt, D. Neil Hayes, Adrian Bivol, Michael S. Lawrence, Raju Kucherlapati, Kelsey Zhu, Wen-Bin Liu, Matthew R. Cooperberg, Houtan Noushmehr, Daniel Crain, Luciano Neder, Lynda Chin, Barry S. Taylor, Jiashan Zhang, Bradley A. Murray, Ginette McKercher, Miruna Balasundaram, Chia Chin Wu, Peter T. Scardino, Suhn K. Rhie, Mark A. Rubin, Jean C. Zenklusen, Ronald Simon, Jaeil Ahn, Markus Graefen, Phillip H. Lai, Michael Ittmann, June M. Chan, Maria J. Merino, Andy Chu, Liming Yang, Charles M. Perou, Steven J.M. Jones, Gad Getz, Stacey Gabriel, Martin L. Ferguson, Jianjiong Gao, Payal Sipahimalani, Lisa Wise, Serghei Stepa, Arvind Rao, Bettina F. Drake, Antonia H. Holway, Armaz Mariamidze, Kiley Graim, Juliann Shih, Guido Sauter, Sarah Minner, Amanda Clarke, Harshad S. Mahadeshwar, Andrew J. Mungall, Alain Piché, Cathy D. Vocke, Sudha Chudamani, Yulia Newton, Davide Prandi, Roeland Verhaak, Ilya Shmulevich, Matthew T. Chang, Sara Sadeghi, Michael Button, Joshua M. Stuart, Anne Breggia, Kristian Cibulskis, Giovanni Ciriello, Paul C. Boutros, Yu Chen, Zhining Wang, Jerome Myers, Heidi Dvinge, Ashutosh Tewari, Scott Morris, Andrea Sorcini, Tara Skelly, Niall M. Corcoran, Michael S. Noble, Anthony J. Costello, Timothy C. Thompson, Eric Chuah, Carolyn M. Hutter, Robert K. Bradley, Fred Saad, John A. Libertino, Reanne Bowlby, John A. Stewart, Shiyun Ling, Gunnar Rätsch, George E. Sandusky, Alexis Sharp, Eric J. Burks, Thomas Zeng, Erin Curley, Charlie Sun, Rajiv Dhir, Yussanne Ma, Huihui Ye, Hui Shen, Nils Gehlenborg, Imelda Tenggara, Travis Sullivan, Tom Bodenheimer, Jia Liu, Christopher D. Andry, Adam Abeshouse, Daniela P. Tirapelli, John N. Weinstein, Kevin Lau, Francisco Sanchez-Vega, Armen Aprikian, Peter W. Laird, Noreen Dhalla, Candace Shelton, Joel Nelson, Bernard Têtu, Darlene Lee, Eleonora Scarlata, Mark Nelson, Steven E. Schumacher, Robert J. Klein, Mark Gerken, Donghui Tan, Semin Lee, John S. Witte, Shaowu Meng, Huandong Sun, Moiz S. Bootwalla, Scott A. Tomlins
المساهمون: Institute for Medical Engineering and Science, Broad Institute of MIT and Harvard, Massachusetts Institute of Technology. Department of Biology, Bradley, Robert K, Carmell, Michelle, Carter, Scott, Chin, Lynda, Cibulskis, Kristian, Getz, Gad Asher, Heiman, David, Lander, Eric Steven, Lin, Pei, Loda, Massimo, Meyerson, Matthew L, Park, Peter J, Seidman, Jonathan, Sougnez, Carrie L, Verhaak, Roel, Voet, Douglas
المصدر: PMC
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USPمصطلحات موضوعية: 0301 basic medicine, Oncology, Male, medicine.medical_specialty, DNA Repair, DNA repair, Urology, MEDLINE, Biology, SPOP, Bioinformatics, Molecular taxonomy, General Biochemistry, Genetics and Molecular Biology, Article, Epigenesis, Genetic, Fusion gene, 03 medical and health sciences, Prostate cancer, Phosphatidylinositol 3-Kinases, 0302 clinical medicine, Prostate, Internal medicine, medicine, Humans, Neoplasm Metastasis, Gene, 030304 developmental biology, Gynecology, 0303 health sciences, Primary (chemistry), business.industry, Prostatic Neoplasms, medicine.disease, 3. Good health, Androgen receptor, Gene Expression Regulation, Neoplastic, GENÉTICA, 030104 developmental biology, medicine.anatomical_structure, Receptors, Androgen, 030220 oncology & carcinogenesis, DNA methylation, Mutation, Cancer research, ras Proteins, Gene Fusion, business, Signal Transduction
الوصف: There is substantial heterogeneity among primary prostate cancers, evident in the spectrum of molecular abnormalities and its variable clinical course. As part of The Cancer Genome Atlas (TCGA), we present a comprehensive molecular analysis of 333 primary prostate carcinomas. Our results revealed a molecular taxonomy in which 74% of these tumors fell into one of seven subtypes defined by specific gene fusions (ERG, ETV1/4, and FLI1) or mutations (SPOP, FOXA1, and IDH1). Epigenetic profiles showed substantial heterogeneity, including an IDH1 mutant subset with a methylator phenotype. Androgen receptor (AR) activity varied widely and in a subtype-specific manner, with SPOP and FOXA1 mutant tumors having the highest levels of AR-induced transcripts. 25% of the prostate cancers had a presumed actionable lesion in the PI3K or MAPK signaling pathways, and DNA repair genes were inactivated in 19%. Our analysis reveals molecular heterogeneity among primary prostate cancers, as well as potentially actionable molecular defects.
National Institutes of Health (U.S.). (grant 5U24CA143799)
National Institutes of Health (U.S.). (grant 5U24CA143835)
National Institutes of Health (U.S.). (grant 5U24CA143840)
National Institutes of Health (U.S.). (grant 5U24CA143843)
National Institutes of Health (U.S.). (grant 5U24CA143845)
National Institutes of Health (U.S.). (grant 5U24CA143848)
National Institutes of Health (U.S.). (grant 5U24CA143858)
National Institutes of Health (U.S.). (grant 5U24CA143866)
National Institutes of Health (U.S.). (grant 5U24CA143867)
National Institutes of Health (U.S.). (grant 5U24CA143882)
National Institutes of Health (U.S.). (grant 5U24CA143883)
National Institutes of Health (U.S.). (grant 5U24CA144025)
National Institutes of Health (U.S.). (grant U54HG003067)
National Institutes of Health (U.S.). (grant U54HG003079)
National Institutes of Health (U.S.). (grant U54HG003273)
National Institutes of Health (U.S.). (grant P30CA16672)وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ffb3a83a71664a609e3d3c1681b25814Test
https://europepmc.org/articles/PMC4695400Test/ -
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المؤلفون: Ricardo Akiyoshi Nakamura, Carlos Roberto Monti, Felipe Amstalden Trevisan, Alexandre Arthur Jacinto
المصدر: Radiologia Brasileira, Vol 42, Iss 2, Pp 75-82 (2009)
مصطلحات موضوعية: lcsh:Medical physics. Medical radiology. Nuclear medicine, Radioterapia, Radiotherapy, Toxicity, lcsh:R895-920, Prostate, Câncer, Toxicidade, Próstata, Prognostic factors, Fatores prognósticos, Cancer
الوصف: OBJETIVO: Reportar resultados de tratamentos do câncer de próstata com radioterapia conformada 3D realizados em uma única instituição. MATERIAIS E MÉTODOS: De julho de 1997 a janeiro de 2002, 285 pacientes consecutivos com câncer de próstata foram submetidos a radioterapia conformada 3D com dose mediana de 7.920 cGy na próstata e analisados retrospectivamente. A distribuição segundo o grupo de risco foi a seguinte: baixo risco - 95 (33,7%); risco intermediário - 66 (23,4%); alto risco - 121 (42,9%) pacientes. RESULTADOS: Em seguimento mediano de 53,6 meses (3,6-95,3 meses), sobrevidas atuariais global, causa específica, livre de metástases a distância e livre de recidiva bioquímica em cinco anos foram de 85,1%, 97,0%, 94,2% e 75,8%, respectivamente. Sobrevidas atuariais livre de toxicidade retal e urinária tardias em cinco anos foram de 96,4% e 91,1%, respectivamente. Ressecção transuretral pré-radioterapia conformada 3D e doses > 70 Gy em 30% do volume da bexiga implicaram maior toxicidade urinária tardia grau 2-3 em cinco anos (p = 0,0002 e p = 0,0264, respectivamente). CONCLUSÃO: A primeira experiência relatada de radioterapia conformada 3D no Brasil permitiu altas doses de radiação, com toxicidades retal e urinária aceitáveis. A existência de ressecção transuretral de próstata pré-radioterapia conformada 3D pode sinalizar maior risco de toxicidade urinária tardia grau 2-3 após irradiação. Restrição da dose < 70 Gy em 30% do volume da bexiga à tomografia de planejamento pode reduzir complicações urinárias tardias.OBJECTIVE: To report the outcomes of 3D conformal radiation therapy for prostate cancer in a single institution. MATERIALS AND METHODS: From July 1997 to January 2002, 285 consecutive patients with prostate cancer were submitted to 3D conformal radiation therapy receiving a median dose of 7,920 cGy to the prostate, and were retrospectively evaluated. The patients distribution according to the level of risk was the following: low risk - 95 (33.7%); intermediate risk - 66 (23.4%); high risk - 121 (42.9%) patients. RESULTS: Median follow-up of 53.6 months (3.6-95.3 months) demonstrated 85.1% actuarial five-year overall survival, 97.0% specific cause survival, 94.2% five-year distant metastasis-free survival, and 75.8% five-year biochemical recurrence-free survival. Rates of five-year actuarial survival free from late rectal and urinary toxicity were 96.4% and 91.1% respectively. Pre-3D conformal radiation therapy transurethral resection of the prostate and doses > 70 Gy in 30% of the bladder volume implied a higher grade 2-3 late urinary toxicity in five years (p = 0.0002 and p = 0.0264, respectively). CONCLUSION: The first experiment with 3D conformal radiation therapy reported in Brazil allowed high radiation doses with acceptable levels of urinary and rectal toxicity. Pre-3D conformal radiation therapy transurethral resection of prostate may determine a higher risk for post-irradiation grade 2-3 late urinary toxicity. At the tomography planning, the reduction of the radiation dose to < 70 Gy in 30% of the bladder volume may reduce the risk for late urinary complications.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doajarticles::25f698e79a4f8bcaa880703378c10542Test
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-39842009000200004Test -
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المؤلفون: Ricardo Pires de Souza, Joel Pinheiro de Brito Júnior, Olger de Souza Tornin, Ademar José de Oliveira Paes Junior, Cristiano Ventorim de Barros, Felipe Amstalden Trevisan, Carlos Neutzling Lehn
المصدر: Radiologia Brasileira, Vol 39, Iss 5, Pp 367-372 (2006)
مصطلحات موضوعية: lcsh:Medical physics. Medical radiology. Nuclear medicine, Magnetic resonance imaging, Tomografia computadorizada, lcsh:R895-920, Nasal sinuses, Nasossinusal, Ressonância magnética, Computed tomography
الوصف: Este estudo propõe-se a avaliar o complexo nasossinusal, a fim de identificar os principais achados e determinar as doenças desta área. A análise precisa da extensão local e disseminação tumoral, dada pela tomografia computadorizada e ressonância magnética, desempenha papel importante no planejamento terapêutico, influenciando também o prognóstico.The aim of this study is to evaluate the sinonasal complex to identify the main findings and to determine the diseases in this area. An accurate analysis of the local extent and tumoral dissemination through computed tomography and magnetic resonance imaging plays a significant role in the therapeutic planning, also affecting the prognosis.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doajarticles::425f5294e6d34d5bade9bb3760ddc8ddTest
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-39842006000500013Test -
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المؤلفون: Ademar José de Oliveira Paes Junior, Cristiano Ventorim de Barros, Olger de Souza Tornin, Felipe Amstalden Trevisan, Carlos Neutzling Lehn, Ricardo Pires de Souza, Joel Pinheiro de Brito Júnior
المصدر: Radiologia Brasileira v.39 n.5 2006
Radiologia Brasileira
Colégio Brasileiro de Radiologia e Diagnóstico por Imagem (CBR)
instacron:CBRمصطلحات موضوعية: Tomografia computadorizada, Radiology, Nuclear Medicine and imaging, Nasossinusal, Ressonância magnética
الوصف: Este estudo propõe-se a avaliar o complexo nasossinusal, a fim de identificar os principais achados e determinar as doenças desta área. A análise precisa da extensão local e disseminação tumoral, dada pela tomografia computadorizada e ressonância magnética, desempenha papel importante no planejamento terapêutico, influenciando também o prognóstico.
وصف الملف: text/html
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6abfdf693994544de77e598502e8e8c1Test
https://doi.org/10.1590/s0100-39842006000500013Test -
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المؤلفون: Felipe Amstalden Trevisan, Luiz Gonzaga Tone, Pamela Viani de Andrade, Rosane Gomes de Paula Queiroz, Augusto Faria Andrade, Elvis Terci Valera
المصدر: Central nervous system agents in medicinal chemistry. 14(2)
مصطلحات موضوعية: Histone Acetyltransferases, Pathology, medicine.medical_specialty, Clinical Trials as Topic, biology, Brain Neoplasms, General Neuroscience, Cancer, medicine.disease, Histone Deacetylase Inhibitors, Neuropsychology and Physiological Psychology, Histone, Treatment Outcome, Acetylation, Gene expression, biology.protein, medicine, Cancer research, Molecular Medicine, Animals, Humans, Histone deacetylase, Epigenetics, Cancer epigenetics, Child
الوصف: Pediatric brain tumors (BT) represent a broad group of malignancies that affect children, displaying different degrees of aggressiveness and prognosis. Current studies demonstrate a crosslink between genetic and epigenetic changes within these tumors. Histone modifications are key elements in the pathogenesis of cancer in general and in brain tumors in particular. It is well documented that at least two classes of enzymes control acetylation of histones: histone acetyltransferases (HATs) and histone deacetylase (HDACs). Transformed HAT or HDAC action was identified in a number of human tumors. It has been hypothesized that HDACs regulate gene expression by deacetylating important genes for cell maintenance. Several HDACs inhibitors have been characterized in the last years and have been shown to promote growth blockage, differentiation and apoptosis in various types of tumors, including glioblastomas, medulloblastomas, neuroblastomas, melanomas, and leukemias. Some of these inhibitors are currently under clinical investigation for different cancer treatments. This review summarizes important mechanisms of histone modifications and discusses recent discoveries with impact on the pre-clinical and clinical field of pediatric brain tumor treatment.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0100b0bca2080030fdf402b8d4e08b4Test
https://pubmed.ncbi.nlm.nih.gov/25388206Test
