المؤلفون: Paccoud, Olivier, Desnos-Ollivier, Marie, Cassaing, Sophie, Boukris-Sitbon, Karine, Alanio, Alexandre, Bellanger, Anne-Pauline, Bonnal, Christine, Bonhomme, Julie, Botterel, Françoise, Bougnoux, Marie-Elisabeth, Brun, Sophie, Chouaki, Taieb, Cornet, Muriel, Dannaoui, Eric, Demar, Magalie, Desbois-Nogard, Nicole, Durieux, Marie-Fleur, Favennec, Loïc, Fekkar, Arnaud, Gabriel, Frederic, Gangneux, Jean-Pierre, Guitard, Juliette, Hasseine, Lilia, Huguenin, Antoine, Le Gal, Solène, Letscher-Bru, Valérie, Mahinc, Caroline, Morio, Florent, Nicolas, Muriel, Rouges, Célia, Cateau, Estelle, Persat, Florence, Poirier, Philippe, Ranque, Stéphane, Roosen, Gabrielle, Roux, Anne-Laure, Sasso, Milène, Lortholary, Olivier, Lanternier, Fanny, Brieu, N, Durand, C, Bertei, D, Bouchara, J, Pihet, M, Bland, S, Bru, J, Pulik, M, Turdu, F Le, Lefrand, H, Ferrand, M, Larrouy, M, Nevez, G, Quinio, D, Bachelier, M, Coustumier, a Le, Carmagnol, F, Rivière, B, Podac, B, Augereau, O, Emond, J, Bacri, J, Berthelot, G, Dalle, F, Vallee, E, Bizet, J, Noussair, L, Herrmann, J, Brocard, C, Guiffault, P, Layet, A, Morel, A, Penn, P, Gigandon, A, Sendid, B, Cornu, M, Prades, N, Fiacre, T Benoit-Cattin A, Levy, S, Pitsch, A, Kiefer, M, Debourgogne, A, Moquet, O, Colot, J, Courtellemont, L, Poisson, D, Laurens, V, Martres, P, Godineau, N, Picot, S, Chassagne, C, Djibo, N, Devallière, R, Camin-Ravenne, A, Bissuel, F, Janvier, F, Eloy, C, Fur, A, Rezzouk, L, Mazars, E, Eloy, O, Chachaty, E, Mihaila, L, Dellion, S, Patey, O, Thouvenot, A, Limousin, L, Desplaces, N, Raguin, G, Gits-Muselli, M

المساهمون: Institut Necker Enfants-Malades (INEM - UM 111 (UMR 8253 / U1151)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté COMUE (UBFC)-Université Bourgogne Franche-Comté COMUE (UBFC), Translational Innovation in Medicine and Complexity / Recherche Translationnelle et Innovation en Médecine et Complexité - UMR 5525 (TIMC ), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique EHESP (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Centre Hospitalier Universitaire de Rennes CHU Rennes = Rennes University Hospital Ponchaillou, Mucoviscidose: physiopathologie et phénogénomique CRSA, Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), CHU Saint-Antoine AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Infections Respiratoires Fongiques (IRF), Université d'Angers (UA)-Université de Brest (UBO), SFR UA 4208 Interactions Cellulaires et Applications Thérapeutiques (ICAT), Université d'Angers (UA), Laboratoire de Parasitologie et Mycologiede CHRU Brest, Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Institut Pasteur Paris (IP), Laboratoire de Parasitologie-Mycologie CHU Angers, Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM), This research was supported by Institut Pasteur and Santé Publique France.

المصدر: ISSN: 2328-8957.

مصطلحات موضوعية: cryptococcal meningitis, cryptococcosis, fungemia, human immunodeficiency virus, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology

الوصف: International audience ; Among 1107 cryptococcosis cases from the French surveillance network (2005–2020), the proportion of HIV-seronegative individuals has recently surpassed that of HIV-seropositive individuals. We observed marked differences in patient characteristics, disease presentations, cryptococcal antigen results, infecting species, and mortality according to HIV serostatus.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/38344129; hal-04473848; https://normandie-univ.hal.science/hal-04473848Test; PUBMED: 38344129

الإتاحة: https://doi.org/10.1093/ofid/ofad658Test
https://normandie-univ.hal.science/hal-04473848Test

المؤلفون: Blaize, Marion, Thizy, Guillaume, Boissonnas, Alexandre, Portalier, Anaïs, Lanternier, Fanny, de La Porte des Vaux, Clémentine, Suarez, Felipe, Bougnoux, Marie-Elisabeth, Guitard, Juliette, Jabet, Arnaud, Stocker, Nicolas, Aoudjhane, Abdelmalek, Roos-Weil, Damien, Fekkar, Arnaud

المصدر: International Journal of Infectious Diseases ; volume 142, page 107000 ; ISSN 1201-9712

مصطلحات موضوعية: Infectious Diseases, Microbiology (medical), General Medicine

الإتاحة: https://doi.org/10.1016/j.ijid.2024.107000Test
https://api.elsevier.com/content/article/PII:S1201971224000717?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S1201971224000717?httpAccept=text/plainTest

المؤلفون: Bastard, Paul, Vazquez, Sara, Liu, Jamin, Laurie, Matthew T, Wang, Chung Yu, Gervais, Adrian, Le Voyer, Tom, Bizien, Lucy, Zamecnik, Colin, Philippot, Quentin, Rosain, Jérémie, Catherinot, Emilie, Willmore, Andrew, Mitchell, Anthea M, Bair, Rebecca, Garçon, Pierre, Kenney, Heather, Fekkar, Arnaud, Salagianni, Maria, Poulakou, Garyphallia, Siouti, Eleni, Sahanic, Sabina, Tancevski, Ivan, Weiss, Günter, Nagl, Laurenz, Manry, Jérémy, Duvlis, Sotirija, Arroyo-Sánchez, Daniel, Paz Artal, Estela, Rubio, Luis, Perani, Cristiano, Bezzi, Michela, Sottini, Alessandra, Quaresima, Virginia, Roussel, Lucie, Vinh, Donald C, Reyes, Luis Felipe, Garzaro, Margaux, Hatipoglu, Nevin, Boutboul, David, Tandjaoui-Lambiotte, Yacine, Borghesi, Alessandro, Aliberti, Anna, Cassaniti, Irene, Venet, Fabienne, Monneret, Guillaume, Halwani, Rabih, Sharif-Askari, Narjes Saheb, Danielson, Jeffrey, Burrel, Sonia, Morbieu, Caroline, Stepanovskyy, Yurii, Bondarenko, Anastasia, Volokha, Alla, Boyarchuk, Oksana, Gagro, Alenka, Neuville, Mathilde, Neven, Bénédicte, Keles, Sevgi, Hernu, Romain, Bal, Antonin, Novelli, Antonio, Novelli, Giuseppe, Saker, Kahina, Ailioaie, Oana, Antolí, Arnau, Jeziorski, Eric, Rocamora-Blanch, Gemma, Teixeira, Carla, Delaunay, Clarisse, Lhuillier, Marine, Le Turnier, Paul, Zhang, Yu, Mahevas, Matthieu, Pan-Hammarström, Qiang, Abolhassani, Hassan, Bompoil, Thierry, Dorgham, Karim, Gorochov, Guy, Laouenan, Cédric, Rodríguez-Gallego, Carlos, Ng, Lisa FP, Renia, Laurent, Pujol, Aurora, Belot, Alexandre, Raffi, François, Allende, Luis M, Martinez-Picado, Javier, Ozcelik, Tayfun, Imberti, Luisa, Notarangelo, Luigi D, Troya, Jesus, Solanich, Xavier, Zhang, Shen-Ying, Puel, Anne, Wilson, Michael R, Trouillet-Assant, Sophie, Abel, Laurent, Jouanguy, Emmanuelle

المصدر: Science Immunology. 7(74)

مصطلحات موضوعية: Biomedical and Clinical Sciences, Immunology, Prevention, Coronaviruses, Pneumonia & Influenza, Lung, Biotechnology, Clinical Research, Immunization, Pneumonia, Vaccine Related, Emerging Infectious Diseases, Infectious Diseases, Coronaviruses Vaccines, Infection, Good Health and Well Being, COVID HGE Consortium, French COVID Study Group, COMET Consortium, Humans, Interferon Type I, RNA, Messenger, Vaccines, Vaccination, COVID-19, SARS-CoV-2, COVID-19 Vaccines, Clinical sciences

الوصف: Life-threatening "breakthrough" cases of critical COVID-19 are attributed to poor or waning antibody (Ab) response to SARS-CoV-2 vaccines in individuals already at risk. Preexisting auto-Abs neutralizing type I IFNs underlie at least 15% of critical COVID-19 pneumonia cases in unvaccinated individuals; their contribution to hypoxemic breakthrough cases in vaccinated people is unknown. We studied a cohort of 48 individuals (aged 20 to 86 years) who received two doses of a messenger RNA (mRNA) vaccine and developed a breakthrough infection with hypoxemic COVID-19 pneumonia 2 weeks to 4 months later. Ab levels to the vaccine, neutralization of the virus, and auto-Abs to type I IFNs were measured in the plasma. Forty-two individuals had no known deficiency of B cell immunity and a normal Ab response to the vaccine. Among them, 10 (24%) had auto-Abs neutralizing type I IFNs (aged 43 to 86 years). Eight of these 10 patients had auto-Abs neutralizing both IFN-α2 and IFN-ω, whereas two neutralized IFN-ω only. No patient neutralized IFN-β. Seven neutralized type I IFNs at 10 ng/ml and three at 100 pg/ml only. Seven patients neutralized SARS-CoV-2 D614G and Delta efficiently, whereas one patient neutralized Delta slightly less efficiently. Two of the three patients neutralizing only type I IFNs at 100 pg/ml neutralized both D614G and Delta less efficiently. Despite two mRNA vaccine inoculations and the presence of circulating Abs capable of neutralizing SARS-CoV-2, auto-Abs neutralizing type I IFNs may underlie a notable proportion of hypoxemic COVID-19 pneumonia cases, highlighting the importance of this particularly vulnerable population.

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/8628s3dxTest
https://escholarship.org/content/qt8628s3dx/qt8628s3dx.pdfTest

المؤلفون: Bastard, Paul, Gervais, Adrian, Le Voyer, Tom, Rosain, Jérémie, Philippot, Quentin, Manry, Jérémy, Michailidis, Eleftherios, Hoffmann, Hans-Heinrich, Eto, Shohei, Garcia-Prat, Marina, Bizien, Lucy, Parra-Martínez, Alba, Yang, Rui, Haljasmägi, Liis, Migaud, Mélanie, Särekannu, Karita, Maslovskaja, Julia, de Prost, Nicolas, Tandjaoui-Lambiotte, Yacine, Luyt, Charles-Edouard, Amador-Borrero, Blanca, Gaudet, Alexandre, Poissy, Julien, Morel, Pascal, Richard, Pascale, Cognasse, Fabrice, Troya, Jesus, Trouillet-Assant, Sophie, Belot, Alexandre, Saker, Kahina, Garçon, Pierre, Rivière, Jacques G, Lagier, Jean-Christophe, Gentile, Stéphanie, Rosen, Lindsey B, Shaw, Elana, Morio, Tomohiro, Tanaka, Junko, Dalmau, David, Tharaux, Pierre-Louis, Sene, Damien, Stepanian, Alain, Megarbane, Bruno, Triantafyllia, Vasiliki, Fekkar, Arnaud, Heath, James R, Franco, José Luis, Anaya, Juan-Manuel, Solé-Violán, Jordi, Imberti, Luisa, Biondi, Andrea, Bonfanti, Paolo, Castagnoli, Riccardo, Delmonte, Ottavia M, Zhang, Yu, Snow, Andrew L, Holland, Steven M, Biggs, Catherine, Moncada-Vélez, Marcela, Arias, Andrés Augusto, Lorenzo, Lazaro, Boucherit, Soraya, Coulibaly, Boubacar, Anglicheau, Dany, Planas, Anna M, Haerynck, Filomeen, Duvlis, Sotirija, Nussbaum, Robert L, Ozcelik, Tayfun, Keles, Sevgi, Bousfiha, Ahmed A, Bakkouri, Jalila El, Ramirez-Santana, Carolina, Paul, Stéphane, Pan-Hammarström, Qiang, Hammarström, Lennart, Dupont, Annabelle, Kurolap, Alina, Metz, Christine N, Aiuti, Alessandro, Casari, Giorgio, Lampasona, Vito, Ciceri, Fabio, Barreiros, Lucila A, Dominguez-Garrido, Elena, Vidigal, Mateus, Zatz, Mayana, van de Beek, Diederik, Sahanic, Sabina, Tancevski, Ivan, Stepanovskyy, Yurii, Boyarchuk, Oksana, Nukui, Yoko, Tsumura, Miyuki, Vidaur, Loreto, Tangye, Stuart G, Burrel, Sonia, Duffy, Darragh, Quintana-Murci, Lluis, Klocperk, Adam

المصدر: Science Immunology. 6(62)

مصطلحات موضوعية: Pneumonia, Lung, Infectious Diseases, Emerging Infectious Diseases, Vaccine Related, Clinical Research, Pneumonia & Influenza, Prevention, Infection, Good Health and Well Being, Adolescent, Adult, Aged, Aged, 80 and over, Antibodies, Neutralizing, Autoantibodies, COVID-19, Case-Control Studies, Child, Child, Preschool, Critical Illness, Humans, Immunoglobulin G, Infant, Infant, Newborn, Interferon Type I, Interferon-alpha, Middle Aged, Young Adult, HGID Lab, COVID Clinicians, COVID-STORM Clinicians, NIAID Immune Response to COVID Group, NH-COVAIR Study Group, Danish CHGE, Danish Blood Donor Study, St. James's Hospital, SARS CoV2 Interest group, French COVID Cohort Study Group, Imagine COVID-Group, Milieu Intérieur Consortium, CoV-Contact Cohort, Amsterdam UMC Covid-19, Biobank Investigators, COVID Human Genetic Effort, CONSTANCES cohort, 3C-Dijon Study, Cerba Health-Care, Etablissement du Sang study group

الوصف: Circulating autoantibodies (auto-Abs) neutralizing high concentrations (10 ng/mL, in plasma diluted 1 to 10) of IFN-α and/or -ω are found in about 10% of patients with critical COVID-19 pneumonia, but not in subjects with asymptomatic infections. We detect auto-Abs neutralizing 100-fold lower, more physiological, concentrations of IFN-α and/or -ω (100 pg/mL, in 1/10 dilutions of plasma) in 13.6% of 3,595 patients with critical COVID-19, including 21% of 374 patients > 80 years, and 6.5% of 522 patients with severe COVID-19. These antibodies are also detected in 18% of the 1,124 deceased patients (aged 20 days-99 years; mean: 70 years). Moreover, another 1.3% of patients with critical COVID-19 and 0.9% of the deceased patients have auto-Abs neutralizing high concentrations of IFN-β. We also show, in a sample of 34,159 uninfected subjects from the general population, that auto-Abs neutralizing high concentrations of IFN-α and/or -ω are present in 0.18% of individuals between 18 and 69 years, 1.1% between 70 and 79 years, and 3.4% >80 years. Moreover, the proportion of subjects carrying auto-Abs neutralizing lower concentrations is greater in a subsample of 10,778 uninfected individuals: 1% of individuals 80 years. By contrast, auto-Abs neutralizing IFN-β do not become more frequent with age. Auto-Abs neutralizing type I IFNs predate SARS-CoV-2 infection and sharply increase in prevalence after the age of 70 years. They account for about 20% of both critical COVID-19 cases in the over-80s, and total fatal COVID-19 cases.

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/1sp3z9cjTest

المؤلفون: Mohammad, Noshine, Normand, Anne-Cécile, Nabet, Cécile, Godmer, Alexandre, Brossas, Jean-Yves, Blaize, Marion, Bonnal, Christine, Fekkar, Arnaud, Imbert, Sébastien, Tannier, Xavier, Piarroux, Renaud

المساهمون: CHU Pitié-Salpêtrière AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), CHU Saint-Antoine AP-HP, AP-HP - Hôpital Bichat - Claude Bernard Paris, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CHU Bordeaux, Laboratoire d'Informatique Médicale et Ingénierie des Connaissances en e-Santé (LIMICS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Sorbonne Paris Nord

المصدر: ISSN: 2076-2607 ; Microorganisms ; https://hal.science/hal-04091115Test ; Microorganisms, 2023, 11 (4), pp.1071. ⟨10.3390/microorganisms11041071⟩.

مصطلحات موضوعية: MALDI TOF, epidemiology, Candida parapsilosis, neural network, artificial intelligence, outbreak, deep learning, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Identifying fungal clones propagated during outbreaks in hospital settings is a problem that increasingly confronts biologists. Current tools based on DNA sequencing or microsatellite analysis require specific manipulations that are difficult to implement in the context of routine diagnosis. Using deep learning to classify the mass spectra obtained during the routine identification of fungi by MALDI-TOF mass spectrometry could be of interest to differentiate isolates belonging to epidemic clones from others. As part of the management of a nosocomial outbreak due to Candida parapsilosis in two Parisian hospitals, we studied the impact of the preparation of the spectra on the performance of a deep neural network. Our purpose was to differentiate 39 otherwise fluconazole-resistant isolates belonging to a clonal subset from 56 other isolates, most of which were fluconazole-susceptible, collected during the same period and not belonging to the clonal subset. Our study carried out on spectra obtained on four different machines from isolates cultured for 24 or 48 h on three different culture media showed that each of these parameters had a significant impact on the performance of the classifier. In particular, using different culture times between learning and testing steps could lead to a collapse in the accuracy of the predictions. On the other hand, including spectra obtained after 24 and 48 h of growth during the learning step restored the good results. Finally, we showed that the deleterious effect of the device variability used for learning and testing could be largely improved by including a spectra alignment step during preprocessing before submitting them to the neural network. Taken together, these experiments show the great potential of deep learning models to identify spectra of specific clones, providing that crucial parameters are controlled during both culture and preparation steps before submitting spectra to a classifier.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/37110493; hal-04091115; https://hal.science/hal-04091115Test; https://hal.science/hal-04091115/documentTest; https://hal.science/hal-04091115/file/microorganisms-11-01071.pdfTest; PUBMED: 37110493; PUBMEDCENTRAL: PMC10146746

الإتاحة: https://doi.org/10.3390/microorganisms11041071Test
https://hal.science/hal-04091115Test
https://hal.science/hal-04091115/documentTest
https://hal.science/hal-04091115/file/microorganisms-11-01071.pdfTest

المؤلفون: Vanker, Martti, Särekannu, Karita, Fekkar, Arnaud, Jørgensen, Sofie Eg, Haljasmägi, Liis, Kallaste, Anne, Kisand, Kalle, Lember, Margus, Peterson, Pärt, Menon, Madhvi, Hussell, Tracy, Knight, Sean, Moore-Stanley, James, Bastard, Paul, Zhang, Shen-Ying, Mogensen, Trine H, Philippot, Quentin, Zhang, Qian, Puel, Anne, Casanova, Jean-Laurent, Kisand, Kai

المصدر: Vanker , M , Särekannu , K , Fekkar , A , Jørgensen , S E , Haljasmägi , L , Kallaste , A , Kisand , K , Lember , M , Peterson , P , Menon , M , Hussell , T , Knight , S , Moore-Stanley , J , Bastard , P , Zhang , S-Y , Mogensen , T H , Philippot , Q , Zhang , Q , Puel , A , Casanova , J-L & Kisand , K 2023 , ' Autoantibodies Neutralizing Type III Interferons Are Uncommon in Patients with Severe ....

مصطلحات موضوعية: Humans, COVID-19, Interferon Lambda, SARS-CoV-2, Autoantibodies, Interferon-alpha, Interferon Type I, Interferons

الوصف: Autoantibodies (AABs) neutralizing type I interferons (IFN) underlie about 15% of cases of critical coronavirus disease 2019 (COVID-19) pneumonia. The impact of autoimmunity toward type III IFNs remains unexplored. We included samples from 1,002 patients with COVID-19 (50% with severe disease) and 1,489 SARS-CoV-2-naive individuals. We studied the prevalence and neutralizing capacity of AABs toward IFNλ and IFNα. Luciferase-based immunoprecipitation method was applied using pooled IFNα (subtypes 1, 2, 8, and 21) or pooled IFNλ1-IFNλ3 as antigens, followed by reporter cell-based neutralization assay. In the SARS-CoV-2-naive cohort, IFNλ AABs were more common (8.5%) than those targeting IFNα2 (2.9%) and were related with older age. In the COVID-19 cohort the presence of autoreactivity to IFNλ did not associate with severe disease [odds ratio (OR) 0.84; 95% confidence interval (CI) 0.40-1.73], unlike to IFNα (OR 4.88; 95% CI 2.40-11.06; P < 0.001). Most IFNλ AAB-positive COVID-19 samples (67%) did not neutralize any of the 3 IFNλ subtypes. Pan-IFNλ neutralization occurred in 5 patients (0.50%), who all suffered from severe COVID-19 pneumonia, and 4 of them neutralized IFNα2 in addition to IFNλ. Overall, AABs to type III IFNs are rarely neutralizing, and do not seem to predispose to severe COVID-19 pneumonia on their own.

العلاقة: https://research.manchester.ac.uk/en/publications/7953380a-c167-4c88-8540-854c0a01ca67Test

الإتاحة: https://doi.org/10.1089/jir.2023.0003Test
https://research.manchester.ac.uk/en/publications/7953380a-c167-4c88-8540-854c0a01ca67Test
http://europepmc.org/abstract/med/37253131Test

المؤلفون: Bastard, Paul, Vazquez, Sara E., Liu, Jamin, Laurie, Matthew T., Wang, Chung Yu, Gervais, Adrian, Voyer, Tom Le, Bizien, Lucy, Zamecnik, Colin, Philippot, Quentin, Rosain, Jérémie, Catherinot, Emilie, Willmore, Andrew, Mitchell, Anthea M., Bair, Rebecca, Garçon, Pierre, Kenney, Heather, Fekkar, Arnaud, Salagianni, Maria, Poulakou, Garyphallia, Siouti, Eleni, Sahanic, Sabina, Tancevski, Ivan, Weiss, Günter, Nagl, Laurenz, Manry, Jérémy, Duvlis, Sotirija, Arroyo-Sánchez, Daniel, Artal, Estela Paz, Rubio, Luis, Perani, Cristiano, Bezzi, Michela, Sottini, Alessandra, Quaresima, Virginia, Roussel, Lucie, Vinh, Donald C., Reyes, Luis Felipe, Garzaro, Margaux, Hatipoglu, Nevin, Boutboul, David, Tandjaoui-Lambiotte, Yacine, Borghesi, Alessandro, Aliberti, Anna, Cassaniti, Irene, Venet, Fabienne, Monneret, Guillaume, Halwani, Rabih, Sharif-Askari, Narjes Saheb, Casanova, Jean Laurent

المصدر: Bastard , P , Vazquez , S E , Liu , J , Laurie , M T , Wang , C Y , Gervais , A , Voyer , T L , Bizien , L , Zamecnik , C , Philippot , Q , Rosain , J , Catherinot , E , Willmore , A , Mitchell , A M , Bair , R , Garçon , P , Kenney , H , Fekkar , A , Salagianni , M , Poulakou , G , Siouti , E , Sahanic , S , Tancevski , I , Weiss , ....

الوصف: Life-threatening "breakthrough"cases of critical COVID-19 are attributed to poor or waning antibody (Ab) response to SARS-CoV-2 vaccines in individuals already at risk. Preexisting auto-Abs neutralizing type I IFNs underlie at least 15% of critical COVID-19 pneumonia cases in unvaccinated individuals; their contribution to hypoxemic breakthrough cases in vaccinated people is unknown. We studied a cohort of 48 individuals (aged 20 to 86 years) who received two doses of a messenger RNA (mRNA) vaccine and developed a breakthrough infection with hypoxemic COVID-19 pneumonia 2 weeks to 4 months later. Ab levels to the vaccine, neutralization of the virus, and auto-Abs to type I IFNs were measured in the plasma. Forty-two individuals had no known deficiency of B cell immunity and a normal Ab response to the vaccine. Among them, 10 (24%) had auto-Abs neutralizing type I IFNs (aged 43 to 86 years). Eight of these 10 patients had auto-Abs neutralizing both IFN-a2 and IFN-w, whereas two neutralized IFN-w only. No patient neutralized IFN-b. Seven neutralized type I IFNs at 10 ng/ml and three at 100 pg/ml only. Seven patients neutralized SARS-CoV-2 D614G and Delta efficiently, whereas one patient neutralized Delta slightly less efficiently. Two of the three patients neutralizing only type I IFNs at 100 pg/ml neutralized both D614G and Delta less efficiently. Despite two mRNA vaccine inoculations and the presence of circulating Abs capable of neutralizing SARS-CoV-2, auto-Abs neutralizing type I IFNs may underlie a notable proportion of hypoxemic COVID-19 pneumonia cases, highlighting the importance of this particularly vulnerable population.

العلاقة: https://pure.au.dk/portal/en/publications/b0f8f5e9-a431-4e04-baea-bacb62e13123Test

الإتاحة: https://doi.org/10.1126/sciimmunol.abp8966Test
https://pure.au.dk/portal/en/publications/b0f8f5e9-a431-4e04-baea-bacb62e13123Test
http://www.scopus.com/inward/record.url?scp=85181178475&partnerID=8YFLogxKTest

المؤلفون: Philippot, Quentin, Fekkar, Arnaud, Gervais, Adrian, Le Voyer, Tom, Boers, Leonoor S., Conil, Clément, Bizien, Lucy, de Brabander, Justin, Duitman, Jan Willem, Romano, Alessia, Rosain, Jérémie, Blaize, Marion, Migaud, Mélanie, Jeljeli, Maxime, Hammadi, Boualem, Desmons, Aurore, Marchal, Astrid, Nossent, Esther J., Saris, Anno, De Vries, Heder, Meijboom, Lilian J., Blok, Siebe G., Schuurman, Alex R., Reijnders, Tom D. Y., Hugenholtz, F., Vallejo, Juan J. Garcia, Bontkes, Hetty, Vlaar, Alexander P. J., Wiersinga, Joost, Lutter, René, van der Poll, Tom, Bogaard, Harm Jan, Kullberg, Robert F. J., Zhang, Shiqi, Heunks, Leo M. A., Tuinman, Pieter Roel, Bonta, Peter I., Abel, Laurent, Al-Muhsen, Saleh, Arias, Andrés A., Bogunovic, Dusan, Bolze, Alexandre, Bousfiha, Ahmed A., Mansouri, Davood, Meyts, Isabelle, de Diego, Rebeca Perez, Sancho-Shimizu, Vanessa, Spaan, András N., Tangye, Stuart G.

المساهمون: National Institutes of Health, NIH Clinical and Translational Science Award, French National Research Agency (ANR) under the “Investments for the Future”, Integrative Biology of Emerging Infectious Diseases Laboratory of Excellence, French Foundation for Medical Research, ANR GENVIR, ANR AABIFNCOV, ANR-RHU COVIFERON Program, FRM, Amsterdam UMC through the fellowship program, European Respiratory Society

المصدر: Journal of Clinical Immunology ; volume 43, issue 6, page 1093-1103 ; ISSN 0271-9142 1573-2592

مصطلحات موضوعية: Immunology, Immunology and Allergy

الوصف: Autoantibodies (auto-Abs) neutralizing type I interferons (IFNs) are found in the blood of at least 15% of unvaccinated patients with life-threatening COVID-19 pneumonia. We report here the presence of auto-Abs neutralizing type I IFNs in the bronchoalveolar lavage (BAL) of 54 of the 415 unvaccinated patients (13%) with life-threatening COVID-19 pneumonia tested. The 54 individuals with neutralizing auto-Abs in the BAL included 45 (11%) with auto-Abs against IFN-α2, 37 (9%) with auto-Abs against IFN-ω, 54 (13%) with auto-Abs against IFN-α2 and/or ω, and five (1%) with auto-Abs against IFN-β, including three (0.7%) with auto-Abs neutralizing IFN-α2, IFN-ω, and IFN-β, and two (0.5%) with auto-Abs neutralizing IFN-α2 and IFN-β. Auto-Abs against IFN-α2 also neutralize the other 12 subtypes of IFN-α. Paired plasma samples were available for 95 patients. All seven patients with paired samples who had detectable auto-Abs in BAL also had detectable auto-Abs in plasma, and one patient had auto-Abs detectable only in blood. Auto-Abs neutralizing type I IFNs are, therefore, present in the alveolar space of at least 10% of patients with life-threatening COVID-19 pneumonia. These findings suggest that these auto-Abs impair type I IFN immunity in the lower respiratory tract, thereby contributing to hypoxemic COVID-19 pneumonia.

الإتاحة: https://doi.org/10.1007/s10875-023-01512-9Test

المؤلفون: Paccoud, Olivier, Desnos-Ollivier, Marie, Cassaing, Sophie, Boukris-Sitbon, Karine, Alanio, Alexandre, Bellanger, Anne-Pauline, Bonnal, Christine, Bonhomme, Julie, Botterel, Françoise, Bougnoux, Marie-Elisabeth, Brun, Sophie, Chouaki, Taieb, Cornet, Muriel, Dannaoui, Eric, Demar, Magalie, Desbois-Nogard, Nicole, Durieux, Marie-Fleur, Favennec, Loïc, Fekkar, Arnaud, Gabriel, Frederic, Gangneux, Jean-Pierre, Guitard, Juliette, Hasseine, Lilia, Huguenin, Antoine, Le Gal, Solène, Letscher-Bru, Valérie, Mahinc, Caroline, Morio, Florent, Nicolas, Muriel, Rouges, Célia, Cateau, Estelle, Persat, Florence, Poirier, Philippe, Ranque, Stéphane, Roosen, Gabrielle, Roux, Anne-Laure, Sasso, Milène, Lortholary, Olivier, Lanternier, Fanny, Brieu, N, Durand, C, Bertei, D, Bouchara, J P, Pihet, M, Bland, S, Bru, J P, Pulik, M, Turdu, F Le, Lefrand, H, Ferrand, M

المساهمون: and Santé Publique

المصدر: Open Forum Infectious Diseases ; volume 11, issue 2 ; ISSN 2328-8957

مصطلحات موضوعية: Infectious Diseases, Oncology

الوصف: Among 1107 cryptococcosis cases from the French surveillance network (2005–2020), the proportion of HIV-seronegative individuals has recently surpassed that of HIV-seropositive individuals. We observed marked differences in patient characteristics, disease presentations, cryptococcal antigen results, infecting species, and mortality according to HIV serostatus.

الإتاحة: https://doi.org/10.1093/ofid/ofad658Test
https://academic.oup.com/ofid/article-pdf/11/2/ofad658/56672607/ofad658.pdfTest

المؤلفون: Mercier, Victor, Letscher-Bru, Valérie, Bougnoux, Marie-Elisabeth, Delhaes, Laurence, Botterel, Francoise, Maubon, Danièle, Dalle, Frédéric, Alanio, Alexandre, Houzé, Sandrine, Dannaoui, Eric, Cassagne, Carole, Cassaing, Sophie, Durieux, Marie-Fleur, Fekkar, Arnaud, Bouchara, Jean-Philippe, Gangneux, Jean-Pierre, Bonhomme, Julie, Dupont, Damien, Costa, Damien, Sendid, Boualem, Chouaki, Taieb, Bourgeois, Nathalie, Huguenin, Antoine, Brun, Sophie, Mahinc, Caroline, Hasseine, Lilia, Le Gal, Solène, Bellanger, Anne-Pauline, Bailly, Eric, Morio, Florent, Nourrisson, Céline, Desbois-Nogard, Nicole, Perraud-Cateau, Estelle, Debourgogne, Anne, Yéra, Hélène, Lachaud, Laurence, Sasso, Milène

المصدر: Clinical Microbiology and Infection ; volume 29, issue 5, page 652.e9-652.e15 ; ISSN 1198-743X

مصطلحات موضوعية: Infectious Diseases, Microbiology (medical), General Medicine

الإتاحة: https://doi.org/10.1016/j.cmi.2022.11.030Test
https://api.elsevier.com/content/article/PII:S1198743X22006036?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S1198743X22006036?httpAccept=text/plainTest