المؤلفون: Fengli Guo, Guiming Fu, Fangxuan Li, Yitong Hua, Zhongyu Wang, Xiangqian Zheng, Jingzhu Zhao, Ming Gao

المصدر: World Journal of Surgical Oncology, Vol 22, Iss 1, Pp 1-14 (2024)

مصطلحات موضوعية: Medullary thyroid carcinoma, Calcitonin, Biochemical persistence, Biochemical recurrence, Structural recurrence, Surgery, RD1-811, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Abstract Background Medullary thyroid carcinoma (MTC) is a malignant tumor with low incidence. Currently, most studies have focused on the prognostic risk factors of MTC, whatever, time kinetic and risk factors related to calcitonin normalization (CN) and biochemical persistence/recurrence (BP) are yet to be elucidated. Methods A retrospective study was conducted for 190 MTC patients. Risk factors related to calcitonin normalization (CN) and biochemical persistence/recurrence (BP) were analyzed. The predictors of calcitonin normalization time (CNT) and biochemical persistent/recurrent time (BPT) were identified. Further, the prognostic roles of CNT and BPT were also demonstrated. Results The 5- and 10-year DFS were 86.7% and 70.2%, respectively. The 5- and 10-year OS were 97.6% and 78.8%, respectively. CN was achieved in 120 (63.2%) patients, whereas BP was presented in 76 (40.0%) patients at the last follow up. After curative surgery, 39 (32.5%) and 106 (88.3%) patients achieved CN within 1 week and 1 month. All patients who failed to achieve CN turned to BP over time and 32/70 of them developed structural recurrence. The median time of CNT and BPT was 1 month (1 day to 84 months) and 6 month (3 day to 63months), respectively. LNR > 0.23 and male gender were independent predictors for CN and BP. LNR > 0.23 (Hazard ratio (HR), 0.24; 95% CI,0.13–0.46; P 0.23 (HR,5.10; 95% CI,2.15–12.11; P 1400ng/L (HR,2.34; 95% CI,1.29–4.25; P = 0.005) for shorter BPT. In survival analysis, primary tumor size > 2 cm (HR, 5.81; 95% CI,2.20-15.38; P 1 month (HR, 5.69; 95% CI, 1.17–27.61; P = 0.031) and multifocality (HR, 3.10; 95% CI, 1.45–6.65; P = 0.004) were independent predictor of DFS. Conclusion Early changes of Ctn after curative surgery can predict the long-term risks of biochemical and structural recurrence, which provide a useful real-time prognostic information. LNR significantly affect the time kinetic of biochemical prognosis. Tumor burden and CNT play a crucial role in MTC survival, the intensity of follow-up must be tailored accordingly.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1477-7819Test

الوصول الحر: https://doaj.org/article/0396d3bb810143b9bbb9098a6707dd5eTest

المؤلفون: Fangxuan Li, Jinli Dou, Lijuan Wei, Shixia Li, Juntian Liu

المصدر: BMC Cancer, Vol 24, Iss 1, Pp 1-7 (2024)

مصطلحات موضوعية: Gastric cancer, Lipid metabolism, The risk, Clinicopathologic features, TNM stage, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Abstract Background The aim of this study was to explore the correlation between biomarkers of lipid metabolism and gastric cancer. Methods 1120 gastric cancer patients and 1134 health examiners enrolled in this study. The clinic data and serum lipid level, including Total cholesterol (TC), Triglyceride (TG), Low-density lipoprotein cholesterol (LDL-C) and High-density lipoprotein cholesterol (HDL-C), were collected. Results Serum TG and LDL-C levels in patients with gastric cancer were higher than those in the control group. HDL-C levels were lower than the control group (P

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2407Test

الوصول الحر: https://doaj.org/article/398b3212bb4e46999667192baf6bb502Test

المؤلفون: Lei Zhang, Wei Zhao, Jinke Huang, Fangxuan Li, Jindong Sheng, Hualin Song, Ying Chen

المصدر: Frontiers in Immunology, Vol 13 (2022)

مصطلحات موضوعية: ovarian cancer, dendritic cell, fusion cell membrane, CpG-ODN, cytotoxic T lymphocytes, Immunologic diseases. Allergy, RC581-607

الوصف: Ovarian cancer (OC) is a malignant tumor that seriously affects women’s health. In recent years, immunotherapy has shown great potential in tumor treatment. As a major contributor of immunotherapy, dendritic cells (DCs) - based tumor vaccine has been demonstrated to have a positive effect in inducing immune responses in animal experiments. However, the effect of tumor vaccines in clinical trials is not ideal. Therefore, it is urgent to improve the existing tumor vaccines for tumor treatment. Here, we developed a fusion cell membrane (FCM) nano-vaccine FCM-NPs, which is prepared by fusing DCs and OC cells and coating the FCM on the poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) loaded with the immune adjuvant CpG-oligodeoxynucleotide (CpG-ODN). The fusion process promoted the maturation of DCs, thus up-regulating the expression of costimulatory molecule CD80/CD86 and accelerating lymph node homing of DCs. Furthermore, FCM-NPs has both the immunogenicity of tumor cells and the antigen presenting ability of DCs, it can stimulate naive T lymphocytes to produce a large number of tumor-specific cytotoxic CD8+ T lymphocytes. FCM-NPs exhibited strong immuno-activating effect both in vitro and in vivo. By establishing subcutaneous transplanted tumor model, patient-derived xenograft tumor model and abdominal metastatic tumor model, FCM-NPs was proved to have the effect of delaying the growth and inhibiting the metastasis of OC. FCM-NPs is expected to become a new tumor vaccine for the treatment of ovarian cancer.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fimmu.2022.828263/fullTest; https://doaj.org/toc/1664-3224Test

الوصول الحر: https://doaj.org/article/7394365bf6864f8786f5a294c67c19cdTest

المؤلفون: Lianrun Huang, Fuxuan Zhang, Fangxuan Li, Yin Jia, Minghua Wang, Xiude Hua, Limin Wang

المصدر: Biosensors, Vol 12, Iss 11, p 1058 (2022)

مصطلحات موضوعية: cypermethrin, monoclonal antibody, colloidal gold, lateral flow immunoassay, Biotechnology, TP248.13-248.65

الوصف: Cypermethrin (CYP) is an insecticide in the pyrethroid family and is used widely in agriculture and for public health purposes. However, CYP has been shown to have negative impacts on reproduction, immunity and nerves in mammals. In this study, a monoclonal antibody (mAb) against CYP was prepared and used to establish an indirect competitive immunosorbent assay (ic-ELISA) and colloidal gold lateral flow immunoassay (LFIA) for the quantitative and qualitative determination of CYP residues in agricultural products. The half inhibition concentration of the ic-ELISA was 2.49 ng/mL, and the cut-off value and visual limit of detection of the LFIA were 0.6 and 0.3 μg/mL, respectively. The recovery rates of the ic-ELISA ranged from 78.8% to 87.6% in tomato, cabbage and romaine lettuce. The qualitative results of LFIA and quantitative results of ic-ELISA and HPLC were in good agreement in blind samples. Overall, the established ic-ELISA and LFIA proved to be accurate and rapid methods for the determination of CYP in agricultural products.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2079-6374/12/11/1058Test; https://doaj.org/toc/2079-6374Test

الوصول الحر: https://doaj.org/article/2bbcc2356f1049349ee925e22f04ae4dTest

المؤلفون: Lihui Zhao, Yiran Mao, Jie Mu, Jing Zhao, Fangxuan Li, Sheng Zhang, Xiaojie Xin

المصدر: BMC Medical Imaging, Vol 20, Iss 1, Pp 1-10 (2020)

مصطلحات موضوعية: Superb microvascular imaging, Parotid gland tumors, Pleomorphic adenoma, Warthin’s tumor, Basal cell adenoma, Medical technology, R855-855.5

الوصف: Abstract Background We compared the ultrasound features, superb microvascular imaging (SMI) and micro vessel density (MVD) of pleomorphic adenoma (PA), Warthin’s tumor (WT) and basal cell adenoma (BCA) to explore the clinic value of SMI in differential diagnosis of benign tumors of parotid gland. Methods The vascular distributions and grade by color doppler flow imaging (CDFI) and SMI, as well as vascular index (VI) of 249 parotid gland masses from 217 patients were analyzed. Results The internal echogenicity of BCA are more homogeneous in comparing with WT and PA(P

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s12880-020-00506-yTest; https://doaj.org/toc/1471-2342Test

الوصول الحر: https://doaj.org/article/1b2de79d10604c8fa78824a6f6e40a8eTest

المؤلفون: Fangxuan Li, Yao Sun, Jinchao Huang, Wengui Xu, Juntian Liu, Zhiyong Yuan

المصدر: Cancer Medicine, Vol 8, Iss 17, Pp 7330-7344 (2019)

مصطلحات موضوعية: dendritic cell, gastric cancer, indoleamine 2,3‐dioxygenase, regulator T cell, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Abstract Background The tumor microenvironment represents an abnormal niche containing numerous factors, such as T cells, dendritic cells (DCs), regulatory T cells (Tregs), and indoleamine 2,3‐dioxygenase (IDO), involved in maintaining immune homeostasis and tolerance. All these factors may influence the choice of therapy and the clinical outcomes. Methods Flow cytometry was performed to identify CD4+/CD8 + T cells and DCs, and immunohistochemistry was used to evaluate IDO and Forkhead Box P3 (Foxp3) expression; these experiments were performed in order to explore the clinical and prognostic significance of CD4/CD8 + T cells, DCs, Tregs, and IDO expression in gastric carcinoma. Results Smaller tumor size was correlated with higher expression levels of peripheral CD4 + T cells (P = .003) and CD8 + T cells (P = .002), and lower IDO expression (P = .044) in tumors. Well‐differentiated gastric carcinomas displayed higher peripheral (P = .029) and tumor‐infiltrating CD4 + T cell (P = .009) populations and a higher tumor‐infiltrating DC1/DC2 ratio (P = .048). Gastric cancer in the early T stages exhibited higher populations of peripheral DC2s (P = .044) and a higher tumor‐infiltrating DC1/DC2 ratio (P = .012). Gastric cancer at the N0 stage had lower tumor‐infiltrating DC2s (P = .032) and a higher DC1/DC2 ratio (P = .037). IDO expression was positively correlated with tumor‐infiltrating Foxp3 + Tregs (P

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-7634Test

الوصول الحر: https://doaj.org/article/8d842ed36808410aaa066e63a16f5912Test

المؤلفون: Fangxuan Li, Jinchao Huang, Juntian Liu, Wengui Xu, Zhiyong Yuan

المصدر: Cancer Cell International, Vol 19, Iss 1, Pp 1-10 (2019)

مصطلحات موضوعية: miRNA 106b, miRNA 93, miRNA 25, Gastric cancer, PCA, PLS-DA, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282, Cytology, QH573-671

الوصف: Abstract Background miRNA 106b~25 cluster were demonstrated to be an oncogene. In previous study, we had analyzed the diagnostic significance of miRNA 106b~25 based on its carcinogenesis effect. The significance of miRNA 106b~25 for prognosis of gastric cancer were not researched. Methods We applied multivariate analysis of PCA, PLS-DA and Cox Regression for clinicopathological features and survival time to explore the significance of miRNA 106b~25 expression in plasma and cancer tissues for gastric cancer. Results The expression of miRNA 106b, miRNA 93 and miRNA 25 in plasma were positively correlated with their expression in tumor tissues. Via PCA analysis, it was found that miRNA 106b~25 expression in plasma and tumor, T, N and TNM stage were correlated with each other. Via PLS-DA analysis, we identified that T, N and TNM stage were important factors for miRNA 106b~25 expression both in plasma and tumor (all VIP value > 1.2). According to loading weights of variables for the first and second components, it was found that the importance of the miRNA 106b~25s expression carried with the progressed stage of gastric cancer. In the survival analysis, COX regression showed that T stage, plasma miRNA 106b and tumor miRNA 93 were significant risk factors for overall survival [HR: 0.400 (0.205–0.780); P = 0.007; HR: 0.371 (0.142–0.969), P = 0.043; 0.295 (0.134–0.650), P = 0.002]. Conclusion Plasma and tumor miRNA 106b~25 expression correlated with T, N and TNM stage. Increased miRNA 106b~25 expression was important characters carried with gastric cancer progression. T stage, plasma miRNA106b and tumor miRNA 93 significant risk factors for overall survival.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s12935-019-0918-7Test; https://doaj.org/toc/1475-2867Test

الوصول الحر: https://doaj.org/article/1e327b5cc87d4da2b30b66942a3fc7c7Test

المؤلفون: Lijuan Li, Nan Wu, Fangxuan Li, Lingmei Li, Lijuan Wei, Juntian Liu

المصدر: Cancer Biology & Medicine, Vol 16, Iss 1, Pp 139-146 (2019)

مصطلحات موضوعية: Breast cancer, pure secretory breast carcinoma, clinicopathologic feature, therapeutics and prognosis, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Objective Secretory breast carcinoma (SBC) is a rare type of breast malignancy, accounting for less than 0.02% of all infiltrating breast malignancies. The pure SBC, a type of SBC without another type of breast malignant neoplasm, is particularly rare. This study aimed to investigate the clinicopathologic and molecular features of pure SBC. Methods The main pathological parameters such as estrogen receptor (ER), progesterone receptor (PR), and human epithelial growth factor receptor 2 (C-erbB-2) were detected by immunohistochemistry (IHC), and the clinicopathologic and prognostic difference were compared with invasive ductal carcinoma (IDC). Fluorescent in situ hybridization (FISH) and reverse transcription polymerase chain reaction (RT-PCR) was performed to identify the ETV6-NTRK3 rearrangement of SBC. Results We found that the positivity rates of ER, PR, C-erbB-2, p53, and S-100 were 47.7% (21/44), 52.3% (23/44), 36.4% (16/44), 27.3% (12/44), and 95.5% (42/44), respectively, which were higher than those reported in previous studies. Special periodic acid-Schiff analysis was performed in 36 patients, and the value of the Ki-67 index ranged from 1% to 50% (mean value: 10%). Interestingly, most patients with pure SBC harbored an ETV6-NTRK3 rearrangement with an 88.6% (39/44) expression rate. Compared with IDC, the tumor size of most patients with SBC was larger than 2 cm (P = 0.024). Ultrasound showed benign lesions, and the total misdiagnosis rate was higher (P = 0.020). Although the pathological classification was mostly triple-negative breast cancers (P = 0.036), there was less metastasis (P = 0.029), and the overall prognosis was better than that of the IDC group. Conclusions Although axillary lymph node metastasis, local recurrence, or distant metastasis may occur, SBC is also considered an indolent neoplasm with a good prognosis. Once diagnosed, surgical treatment should be performed as soon as possible, followed by appropriate adjuvant chemotherapy, irradiation, and endocrine therapies.

وصف الملف: electronic resource

العلاقة: http://www.cancerbiomed.org/index.php/cocr/article/view/1355Test; https://doaj.org/toc/2095-3941Test

الوصول الحر: https://doaj.org/article/c9da34db7cba47c8b39dc69d97f5dfedTest

المؤلفون: Jiali Mu, Xiaofeng Liang, Fangxuan Li, Juntian Liu, Sheng Zhang, Jing Tian

المصدر: Cancer Biology & Medicine, Vol 15, Iss 2, Pp 171-177 (2018)

مصطلحات موضوعية: Extranodal extension, papillary thyroid cancer, ultrasound, lymph node metastasis, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Objective: Extranodal extension in cervical lymph nodes is an important risk factor for the progression and prognosis of papillary thyroid cancer. The purpose of this study was to identify the common and characteristic preoperative ultrasonography features that are associated with the pathologic extranodal extension of metastatic papillary thyroid carcinoma.Methods: We retrospectively assessed and compared clinicopathologic and ultrasound features between 60 papillary thyroid cancer patients with extranodal extension and 120 control patients with papillary thyroid cancer without extranodal extension. Results: With respect to the pathological N stage and clinicopathologic features, N1b stage papillary thyroid carcinomas were more frequently found in patients who were extranodal extension-positive, in comparison with those who were extranodal extension-negative (78.3% vs. 63.3%, P=0.043). Extranodal extension was detected most frequently in level VI cervical lymph nodes (48.7%). In our univariate analysis of patients with papillary thyroid carcinoma, cervical lymph nodes with extranodal extension showed higher incidences of node matting, microcalcification, cystic area, aspect ratio POR): 4.751, 95% confidence interval (CI): 1.212~18.626, P=0.025; OR: 2.707, 95% CI: 1.127~6.502, P=0.026].Conclusions: Common ultrasound features may indicate the presence of extranodal extension in patients with metastatic cervical lymph nodes of papillary thyroid carcinoma.

وصف الملف: electronic resource

العلاقة: http://www.cancerbiomed.org/index.php/cocr/article/view/1130Test; https://doaj.org/toc/2095-3941Test

الوصول الحر: https://doaj.org/article/a90266be09a0432c9d868026b3495440Test

المؤلفون: Lei Zhang, Ying Chen, Fangxuan Li, Lewen Bao, Wenxin Liu

المصدر: Frontiers in Immunology, Vol 10 (2019)

مصطلحات موضوعية: PD-L1, Atezolizumab, angiogenesis, Bevacizumab, ovarian cancer, Immunologic diseases. Allergy, RC581-607

الوصف: The AURELIA trial demonstrated that adding Bevacizumab to chemotherapy significantly improved progression-free survival (PFS) for platinum resistant recurrent ovarian cancer. Recently, immunotherapy also presented potential anti-tumor effects in several malignant solid tumors. This study aimed to investigate whether combining anti-PD-L1 Atezolizumab with BEV may have a synergistic effect and enhance the efficacy of both treatments in cisplatin resistant epithelial ovarian cancer (CREOC). We retrospectively analyzed 124 epithelial ovarian cancer (EOC) patients from Gynecologic Oncology Department of Tianjin Cancer Hospital between January 2013 and June 2018, who all were diagnosed with cisplatin resistance due to progressing

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/article/10.3389/fimmu.2019.00867/fullTest; https://doaj.org/toc/1664-3224Test

الوصول الحر: https://doaj.org/article/6db39fcdf6ed4f92b78c299c500580a6Test