المؤلفون: Yan, Ruojin, Zhang, Hong, Ma, Yuanzhu, Lin, Ruifu, Zhou, Bo, Zhang, Tao, Fan, Chunmei, Zhang, Yuxiang, Wang, Zetao, Fang, Tianshun, Yin, Zi, Cai, Youzhi, Ouyang, Hongwei, Chen, Xiao

المساهمون: Fundamental Research Funds for the Central Universities, Natural Science Foundation of Zhejiang Province, National Natural Science Foundation of China, National Basic Research Program of China

المصدر: Research ; volume 2022 ; ISSN 2639-5274

الوصف: The myotendinous junction (MTJ) is a complex and special anatomical area that connects muscles and tendons, and it is also the key to repairing tendons. Nevertheless, the anatomical structure and connection structure of MTJ, the cluster and distribution of cells, and which cells are involved in repairing the tissue are still unclear. Here, we analyzed the cell subtype distribution and function of human MTJ at single-cell level. We identified four main subtypes, including stem cell, muscle, tendon, and muscle-tendon progenitor cells (MTP). The MTP subpopulation, which remains the characteristics of stem cells and also expresses muscle and tendon marker genes simultaneously, may have the potential for bidirectional differentiation. We also found the muscle-tendon progenitor cells were distributed in the shape of a transparent goblet; muscle cells first connect to the MTP and then to the tendon. And after being transplanted in the MTJ injury model, MTP exhibited strong regenerative capability. Finally, we also demonstrated the importance of mTOR signaling for MTP maintenance by in vitro addition of rapamycin and in vivo validation using mTOR-ko mice. Our research conducted a comprehensive analysis of the heterogeneity of myotendinous junction, discovered a special cluster called MTP, provided new insights into the biological significance of myotendinous junction, and laid the foundation for future research on myotendinous junction regeneration and restoration.

الإتاحة: https://doi.org/10.34133/2022/9760390Test
http://downloads.spj.sciencemag.org/research/2022/9760390.pdfTest
http://downloads.spj.sciencemag.org/research/2022/9760390.xmlTest

المؤلفون: Yu, Shaoyang, Yang, Yating, Hu, Gaoya, Wang, Wei, Zhuang, Wei, Wu, Yong, Huang, Rongfu, Zhang, Yongquan, Gong, Sisi, Fan, Chunmei

المصدر: Analytical Letters; 2023, Vol. 56 Issue 18, p2910-2920, 11p

مصطلحات موضوعية: ALKALINE phosphatase, ACID phosphatase, FOURIER transform infrared spectroscopy, DUAL fluorescence, TRANSMISSION electron microscopy

مستخلص: A novel fluorescence and colorimetric dual probe based upon the inner filter effect (IFE) is reported to selectively and sensitively determine alkaline phosphatase (ALP). First, up-conversion nanoparticles (UCNPs) were prepared via a facile hydrothermal method and characterized by transmission electron microscopy (TEM), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FT-IR), and zeta potential. Under near infrared (NIR) excitation at 980 nm, the prepared nanoparticles emit green light at 541 nm. The absorption band of p-phenylenediamine (PPD) overlaps the emission of UCNPs, which effectively quenches the fluorescence of UCNPs. However, ascorbic acid (AA) generated from the reaction of ascorbic acid 2-phosphate (AAP) and ALP impedes the oxidation of PPD and the fluorescence is restored. Based on this change, a "turn-on" sensor was used to determine AA and ALP with the limits of detection of 2.49 μM and 0.018 U/L, respectively. The constructed assay was applied to determine ALP in serum and the recoveries were from 93.3% to 101.3% with a relative standard deviation from 1.1% to 3.3%. A facile and efficient approach is reported for the determination ALP and offers a theoretical basis for the related diseases. [ABSTRACT FROM AUTHOR]

: Copyright of Analytical Letters is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Wang, Yian, Yan, Qijia, Fan, Chunmei, Mo, Yongzhen, Wang, Yumin, Li, Xiayu, Liao, Qianjin, Guo, Can, Li, Guiyuan, Zeng, Zhaoyang, Xiong, Wei, Huang, He

المصدر: SCIENCE CHINA Life Sciences; Nov2023, Vol. 66 Issue 11, p2515-2526, 12p

مستخلص: Cancer is one of the leading causes of human death worldwide. Treatment of cancer exhausts significant medical resources, and the morbidity and mortality caused by cancer is a huge social burden. Cancer has therefore become a serious economic and social problem shared globally. As an increasingly prevalent disease in China, cancer is a huge challenge for the country's healthcare system. Based on recent data published in the Journal of the National Cancer Center on cancer incidence and mortality in China in 2016, we analyzed the current trends in cancer incidence and changes in cancer mortality and survival rate in China. And also, we examined several key risk factors for cancer pathogenesis and discussed potential countermeasures for cancer prevention and treatment in China. [ABSTRACT FROM AUTHOR]

: Copyright of SCIENCE CHINA Life Sciences is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Qu, Hongke, Fan, Chunmei, Chen, Mingjian, Zhang, Xiangyan, Yan, Qijia, Wang, Yumin, Zhang, Shanshan, Gong, Zhaojian, Shi, Lei, Li, Xiayu, Liao, Qianjin, Xiang, Bo, Zhou, Ming, Guo, Can, Li, Guiyuan, Zeng, Zhaoyang, Wu, Xu, Xiong, Wei

المساهمون: National Natural Science Foundation of China, Overseas Expertise Introduction Project for Discipline Innovation, Natural Science Foundation of Hunan Province, the Fundamental Research Funds for the Central Universities of Central South University

المصدر: Journal of Nanobiotechnology ; volume 19, issue 1 ; ISSN 1477-3155

مصطلحات موضوعية: Pharmaceutical Science, Applied Microbiology and Biotechnology, Biomedical Engineering, Molecular Medicine, Medicine (miscellaneous), Bioengineering

الوصف: The cyclic signal amplification technology has been widely applied for the ultrasensitive detection of many important biomolecules, such as nucleic acids, proteins, enzymes, adenosine triphosphate (ATP), metal ions, exosome, etc. Due to their low content in the complex biological samples, traditional detection methods are insufficient to satisfy the requirements for monitoring those biomolecules. Therefore, effective and sensitive biosensors based on cyclic signal amplification technology are of great significance for the quick and simple diagnosis and treatment of diseases. Fluorescent biosensor based on cyclic signal amplification technology has become a research hotspot due to its simple operation, low cost, short time, high sensitivity and high specificity. This paper introduces several cyclic amplification methods, such as rolling circle amplification (RCA), strand displacement reactions (SDR) and enzyme-assisted amplification (EAA), and summarizes the research progress of using this technology in the detection of different biomolecules in recent years, in order to provide help for the research of more efficient and sensitive detection methods. Graphical Abstract

الإتاحة: https://doi.org/10.1186/s12951-021-01149-zTest

المؤلفون: Mo, Yongzhen, Wang, Yumin, Zhang, Shuai, Xiong, Fang, Yan, Qijia, Jiang, Xianjie, Deng, Xiangying, Wang, Yian, Fan, Chunmei, Tang, Le, Zhang, Shanshan, Gong, Zhaojian, Wang, Fuyan, Liao, Qianjin, Guo, Can, Li, Yong, Li, Xiaoling, Li, Guiyuan, Zeng, Zhaoyang, Xiong, Wei

المساهمون: national natural science foundation of china, overseas expertise introduction project for discipline innovation, natural science foundation of hunan province, Fundamental Research Funds for Central Universities of the Central South University

المصدر: Molecular Cancer ; volume 20, issue 1 ; ISSN 1476-4598

مصطلحات موضوعية: Cancer Research, Oncology, Molecular Medicine

الوصف: Background Circular RNAs (circRNAs) are widely expressed in human cells and are closely associated with cancer development. However, they have rarely been investigated in the context of nasopharyngeal carcinoma (NPC). Methods We screened a new circRNA, circRNF13 , in NPC cells using next-generation sequencing of mRNA. Reverse transcription polymerase chain reaction and RNA fluorescence in situ hybridization were used to detect circRNF13 expression in 12 non-tumor nasopharyngeal epithelial (NPE) tissues and 36 NPC samples. Cell proliferation was detected using MTT and flow cytometry assays, and colony formation capability was detected using colony formation assays. Cell migration and invasion were analyzed using wound-healing and Transwell assays, respectively. Cell glycolysis was analyzed using the Seahorse glycolytic stress test. Glucose transporter type 1 (GLUT1) ubiquitination and SUMOylation modifications were analyzed using co-immunoprecipitation and western blotting. CircRNF13 and Small Ubiquitin-like Modifier 2 (SUMO2) interactions were analyzed using RNA pull-down and luciferase reporter assays. Finally, to test whether circRNF13 inhibited NPC proliferation and metastasis in vivo, we used a xenograft nude mouse model generated by means of subcutaneous or tail vein injection. Results We found that circRNF13 was stably expressed at low levels in NPC clinical tissues and NPC cells. In vitro and in vivo experiments showed that circRNF13 inhibited NPC proliferation and metastasis. Moreover, circRNF13 activated the SUMO2 protein by binding to the 3′- Untranslated Region (3′-UTR) of the SUMO2 gene and prolonging the half-life of SUMO2 mRNA. Upregulation of SUMO2 promotes GLUT1 degradation through SUMOylation and ubiquitination of GLUT1, which regulates the AMPK-mTOR pathway by inhibiting glycolysis, ultimately resulting in the proliferation and metastasis of NPC. Conclusions Our results revealed that a novel circRNF13 plays an important role in the development of NPC through the circRNF13 -SUMO2-GLUT1 ...

الإتاحة: https://doi.org/10.1186/s12943-021-01409-4Test

المؤلفون: Li, Miao, Chen, Rong, Ji, Baoyan, Fan, Chunmei, Wang, Guanying, Yue, Chenli, Li, Guoquan

المصدر: Public Health Genomics ; volume 24, issue 3-4, page 189-198 ; ISSN 1662-4246 1662-8063

مصطلحات موضوعية: Genetics (clinical), Public Health, Environmental and Occupational Health

الوصف: Background: We aimed to explore the relation of XPD and XPF variants with non-small cell lung cancer (NSCLC) risk and the effect of these variants on the sensitivity to cisplatin-based chemotherapy among the Chinese Han population in high-altitude areas. Methods: Eight single-nucleotide polymorphisms (SNPs) in XPD and XPF were genotyped by Agena MassARRAY platform among 506 NSCLC cases and 510 healthy controls. Correlation of XPD and XPF gene polymorphisms with NSCLC susceptibility and the response of cis­platin-based chemotherapy were analyzed with logistic regression by calculating odds ratios (ORs) and 95% confidence intervals (CIs). Results: XPD rs13181 (OR = 1.53, 95% CI: 1.04–2.24, p = 0.029) and rs1052555 (OR = 1.63, 95% CI: 1.05–2.53, p = 0.029) possibly contributed to the increased risk of lung adenocarcinoma, while XPD rs238406 (OR = 0.63, 95% CI: 0.43–0.94, p = 0.024) was a protective factor for lung squamous cell carcinoma. Age, gender, BMI, smoking, and drinking might affect the correlation of XPD and XPF polymorphisms with NSCLC risk. More importantly, XPD rs13181 (OR = 2.91, p = 0.015), XPD rs1052555 (OR = 2.67, p = 0.022), and XPF rs231127 (OR = 4.15, p = 0.008) were associated with treatment response in NSCLC patients underwent cisplatin-based chemotherapy. Conclusion: This study found that XPD and XPF variants might contribute to NSCLC risk and the response of cisplatin-based chemotherapy among the Chinese Han population in high-altitude areas.

الإتاحة: https://doi.org/10.1159/000512641Test
https://www.karger.com/Article/Pdf/512641Test

المؤلفون: Yan, Ruojin, Fan, Chunmei, Yin, Zi, Wang, Tingzhang, Chen, Xiao

المساهمون: Fundamental Research Funds for the Central Universities, National key research and development program of China, NSFC, Zhejiang Provincial Natural Science Foundation of China, National Basic Research Program of China, National Natural Science Foundation of China

المصدر: Stem Cells ; volume 39, issue 5, page 511-521 ; ISSN 1066-5099 1549-4918

مصطلحات موضوعية: Cell Biology, Developmental Biology, Molecular Medicine

الوصف: When used in cell therapy and regenerative medicine strategies, stem cells have potential to treat many previously incurable diseases. However, current application methods using stem cells are underdeveloped, as these cells are used directly regardless of their culture medium and subgroup. For example, when using mesenchymal stem cells (MSCs) in cell therapy, researchers do not consider their source and culture method nor their application angle and function (soft tissue regeneration, hard tissue regeneration, suppression of immune function, or promotion of immune function). By combining machine learning methods (such as deep learning) with data sets obtained through single-cell RNA sequencing (scRNA-seq) technology, we can discover the hidden structure of these cells, predict their effects more accurately, and effectively use subpopulations with differentiation potential for stem cell therapy. scRNA-seq technology has changed the study of transcription, because it can express single-cell genes with single-cell anatomical resolution. However, this powerful technology is sensitive to biological and technical noise. The subsequent data analysis can be computationally difficult for a variety of reasons, such as denoising single cell data, reducing dimensionality, imputing missing values, and accounting for the zero-inflated nature. In this review, we discussed how deep learning methods combined with scRNA-seq data for research, how to interpret scRNA-seq data in more depth, improve the follow-up analysis of stem cells, identify potential subgroups, and promote the implementation of cell therapy and regenerative medicine measures.

الإتاحة: https://doi.org/10.1002/stem.3336Test
https://academic.oup.com/stmcls/article-pdf/39/5/511/42605573/stmcls_39_5_511.pdfTest

المؤلفون: Zhou, Shan, Fan, Chunmei, Zeng, Zhaoyang, Young, Ken H., Li, Yong

المصدر: Frontiers in Cell and Developmental Biology ; volume 9 ; ISSN 2296-634X

الوصف: Immunotherapy, including immune checkpoint blockade and chimeric antigen receptor T cells, is one of the most promising approaches to treat cancer. Vaccines have been effective in preventing cancers like liver cancer and cervical cancer with a viral etiology. Instead of preventing disease, therapeutic cancer vaccines mobilize the immune system to attack existing cancer. p53 is dysregulated in the majority of human cancers and is a highly promising target for cancer vaccines. Over twenty clinical trials have targeted p53 in malignant diseases using vaccines. In this work, we review the progress of vaccinations with p53 or its peptides as the antigens and summarize the clinical and immunological effects of p53-targeting vaccines from clinical trials. The delivery platforms include p53 peptides, viral vectors, and dendritic cells pulsed with short peptides or transduced by p53-encoding viruses. These studies shed light on the feasibility, safety, and clinical benefit of p53 vaccination in select groups of patients, implicating that p53-targeting vaccines warrant further investigations in experimental animals and human studies.

الإتاحة: https://doi.org/10.3389/fcell.2021.762796Test

المؤلفون: Fan, Chunmei

المصدر: 2023 3rd International Conference on Educational Technology (ICET)

الإتاحة: https://doi.org/10.1109/icet59358.2023.10424394Test
http://xplorestaging.ieee.org/ielx7/10424030/10424021/10424394.pdf?arnumber=10424394Test

المؤلفون: Yin, Zi, Lin, Junxin, Yan, Ruojin, Liu, Richun, Liu, Mengfei, Zhou, Bo, Zhou, Wenyan, An, Chengrui, Chen, Yangwu, Hu, Yejun, Fan, Chunmei, Zhao, Kun, Wu, Bingbing, Zou, Xiaohui, Zhang, Jin, El‐Hashash, Ahmed H., Chen, Xiao, Ouyang, Hongwei

المصدر: Advanced Science ; volume 7, issue 23 ; ISSN 2198-3844 2198-3844

الإتاحة: https://doi.org/10.1002/advs.202070129Test