المؤلفون: Encinas, Micaela, Ferrara Muñiz, Ximena, Ayelén Sammarruco, Romina, Ruiz Menna, Victoria, Javier Garro, Carlos, Delgado, Fernando, Macías, Analía, Magnano, Gabriel, Zumárraga, Martín José, Garbaccio, Sergio Gabriel, Eirin, María Emilia

المصدر: Frontiers in Veterinary Science; 2024, p1-6, 6p

مصطلحات موضوعية: TUBERCULIN test, MYCOBACTERIUM bovis, SKIN tests, COW testing, TUBERCULOSIS in cattle

مستخلص: Ante-mortem diagnosis of bovine tuberculosis (bTB) is based mainly on the tuberculin skin test (TST) and the ...-IFN release assay (IGRA). Some infected animals escape screening tests, thus, limit herd sanitation. Previous reports have suggested a predominant pattern of multi-organ lesions attributable to Mycobacterium bovis (the causative agent of bTB) bacteraemia. A case--control study was conducted to investigate blood PCR as an alternative tool for improving ante-mortem detection of TST false-negative bovines. Cases comprised 70 TST false-negative bovines (cases), which were serology positive, and controls included 81 TST positive bovines; all of them confirmed as infected with M. bovis. Detection of the IS6110 target through touchdown blood-PCR (IS6110 TD-PCR) was performed. The positivity of the blood-PCR was 27.2% in the control group. This performance was similar to the 15% obtained among cases (p = 0.134). Most cases identified by the IS6110 TD-PCR exhibited focalized lesions (p = 0.002). Results demonstrated that blood-PCR could detect TST false-negative cattle, even if they are negative for IGRA. Considering that cases exhibited humoral response to M. bovis, further studies conducted in a pre-serological stage could provide evidence about the real contribution of the technique in herds. [ABSTRACT FROM AUTHOR]

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المؤلفون: Garro, Carlos J., Morici, Gabriel E., Tomazic, Mariela L., Vilte, Daniel, Encinas, Micaela, Vega, Celina, Bok, Marina, Parreño, Viviana, Schnittger, Leonhard

المساهمون: Instituto Nacional de Tecnología Agropecuaria

المصدر: Veterinary Parasitology: Regional Studies and Reports ; volume 24, page 100567 ; ISSN 2405-9390

مصطلحات موضوعية: General Veterinary, Parasitology

الإتاحة: https://doi.org/10.1016/j.vprsr.2021.100567Test
https://api.elsevier.com/content/article/PII:S2405939021000393?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2405939021000393?httpAccept=text/plainTest

المؤلفون: Encinas, Micaela, Marfil, Maria Jimena, Garbaccio, Sergio Gabriel, Barandiaran, Soledad, Huertas, Pablo Sebastian, Morsella, Claudia Graciela, Macias, Analía, Magnano, Gabriel, Zapata, L., Bigi, Fabiana, Cataldi, Angel Adrian, Paolicchi, Fernando, Zumarraga, Martin Jose, Eirin, Maria Emilia

المصدر: Tuberculosis 111: 143–146 (Julio 2018)

مصطلحات موضوعية: Mycobacterium bovis Infections, Polymorphism, Antigens, Isolation Techniques, Infección por Mycobacterium bovis, Polimorfismo, Mycobacterium bovis, Antígenos, Técnicas de Aislamiento, Bovine Tuberculosis, Tuberculosis Bovina

الوصف: ESAT-6, CFP-10 and EspC are virulence factors that have been extensively assayed for bovine and human tuberculosis diagnosis due their potent T-cell inducing activities. While polymorphisms of ESAT-6 and CFP-10 were analyzed, with the description of CFP-10 variants in M. tuberculosis, this fact has not been explored in M. bovis field isolates. The coding sequences of esxA (ESAT-6), esxB (CFP-10) and mb3645c (EspC) from 58 M. bovis strains exhibiting genomic variability (spoligotyping) were analyzed. Two genes –esxA and esxB – remained invariant while mb3645c exhibited one synonymous polymorphism (G to A mutation, position 66bp) in one isolate, compared to M. bovis AF2122/97 reference strain. All isolates exhibited a synonymous nucleotide polymorphism simultaneously (G to A mutation, position 255bp), compared to M. tuberculosis H37Rv reference strain. This study confirms the high conservation for ESAT-6, CFP-10 and EspC in local M. bovis field isolates and reinforce the use of these three antigens in the diagnosis of bovine tuberculosis. Further studies should be performed to globally confirm these findings. ; Instituto de Biotecnología ; Fil: Encinas, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Marfil, María Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina ; Fil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Huertas, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina ; Fil: Macias, Analía. Universidad Nacional de ...

وصف الملف: application/pdf

العلاقة: info:eu-repograntAgreement/INTA/PNBIO/1131034/AR./Inmunología molecular y genómica funcional aplicadas a interacciones patógeno hospedador de interés pecuario.; info:eu-repograntAgreement/INTA/PNSA/1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional.; https://www.sciencedirect.com/science/article/pii/S1472979217304924?via%3DihubTest; http://hdl.handle.net/20.500.12123/3664Test; https://doi.org/10.1016/j.tube.2018.06.007Test

الإتاحة: https://doi.org/20.500.12123/3664Test
https://doi.org/10.1016/j.tube.2018.06.007Test
https://hdl.handle.net/20.500.12123/3664Test
https://www.sciencedirect.com/science/article/pii/S1472979217304924?via%3DihubTest

المؤلفون: Garro, Carlos, Morici, Gabriel Edgardo, Tomazic, Mariela Luján, Vilte, Daniel Alejandro, Encinas, Micaela, Vega, Celina Guadalupe, Bok, Marina, Parreño, Gladys Viviana, Schnittger, Leonhard

مصطلحات موضوعية: CALF DIARRHEA, CRYPTOSPORIDIUM PARVUM, CRYPTOSPORIDIUM SPP, ENTEROPATHOGENS, ODDS RATIO, ROTAVIRUS, https://purl.org/becyt/ford/4.3Test, https://purl.org/becyt/ford/4Test

الوصف: Cryptosporidiosis of neonatal dairy calves causes diarrhea, resulting in important economic losses. In Argentina, prevalence values of Cryptosporidium spp. and other enteropathogens such as group A rotavirus (RVA), bovine coronavirus (BCoV) and enterotoxigenic Escherichia coli (ETEC, endotoxin STa+), have been independently studied in different regions. However, an integrative epidemiological investigation on large-scale farms has not been carried out. In this study, fecal samples (n = 908) were randomly collected from diarrheic and healthy calves from 42 dairy farms, and analyzed for the presence of Cryptosporidium spp., RVA, BCoV, ETEC (STa+) and Salmonella spp. In all sampled dairy farms, dams had been vaccinated against rotavirus and gram-negative bacteria to protect calves against neonatal diarrhea. The proportion of calves shedding Cryptosporidium spp., RVA, and BCoV in animals younger than 20 days of age were 29.8%, 12.4% and 6.4%, and in calves aged between 21 and 90 days, 5.6%, 3.9%, and 1.8%, respectively. ETEC was absent in the younger, and occurred only sporadically in the older group (0.9%), whereas Salmonella spp. was absent in both. The observed sporadic finding or even absence of bacterial pathogens might be explained by the frequent use of parenteral antibiotics in 25.3% and 6.5% of the younger and the older group of calves, respectively, within 2 days prior to sampling and/or vaccination of dams against gram-negative bacteria. Diarrhea was observed in 28.8% (95% CI, 24.7–32.8%) of the younger calves and 11.7% (95% CI, 9.1–15.5%) of the older calves. Importantly, Cryptosporidium spp. (odds ratio (OR) = 5.7; 95% CI, 3.3–9.9; p < 0.0001) and RVA (OR = 2.5; 95% CI, 1.2–5.1; p < 0.05) were both found to be risk factors for diarrhea in calves younger than 20 days old. Based on its high prevalence and OR, our results strongly suggest that Cryptosporidium spp. is the principal causative factor for diarrhea in the group of neonatal calves, whereas RVA seems to play a secondary role in the etiology ...

وصف الملف: application/pdf

العلاقة: info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2405939021000393Test; http://hdl.handle.net/11336/162148Test; Garro, Carlos; Morici, Gabriel Edgardo; Tomazic, Mariela Luján; Vilte, Daniel Alejandro; Encinas, Micaela; et al.; Occurrence of Cryptosporidium and other enteropathogens and their association with diarrhea in dairy calves of Buenos Aires province, Argentina; Elsevier Science; Veterinary Parasitology: Regional Studies and Reports; 24; 100567; 4-2021; 1-7; CONICET Digital; CONICET

الإتاحة: https://doi.org/10.1016/j.vprsr.2021.100567Test
http://hdl.handle.net/11336/162148Test

المؤلفون: Encinas, Micaela, Marfil, Maria Jimena, Garbaccio, Sergio Gabriel, Barandiaran, Soledad, Huertas, Pablo Sebastián, Morsella, C., Macías, A., Magnano, Gabriel Gustavo, Zapata, L., Bigi, Fabiana, Cataldi, Ángel Adrián, Paolicchi, Fernando Alberto, Zumárraga, Martín José, Eirin, Maria Emilia

مصطلحات موضوعية: BOVINE TUBERCULOSIS, CFP-10, ESAT-6, ESPC, FIELD ISOLATES, MYCOBACTERIUM BOVIS, POLYMORPHISM, https://purl.org/becyt/ford/4.3Test, https://purl.org/becyt/ford/4Test

الوصف: ESAT-6, CFP-10 and EspC are virulence factors that have been extensively assayed for bovine and human tuberculosis diagnosis due their potent T-cell inducing activities. While polymorphisms of ESAT-6 and CFP-10 were analyzed, with the description of CFP-10 variants in M. tuberculosis, this fact has not been explored in M. bovis field isolates. The coding sequences of esxA (ESAT-6), esxB (CFP-10) and mb3645c (EspC) from 58 M. bovis strains exhibiting genomic variability (spoligotyping) were analyzed. Two genes –esxA and esxB – remained invariant while mb3645c exhibited one synonymous polymorphism (G to A mutation, position 66bp) in one isolate, compared to M. bovis AF2122/97 reference strain. All isolates exhibited a synonymous nucleotide polymorphism simultaneously (G to A mutation, position 255bp), compared to M. tuberculosis H37Rv reference strain. This study confirms the high conservation for ESAT-6, CFP-10 and EspC in local M. bovis field isolates and reinforce the use of these three antigens in the diagnosis of bovine tuberculosis. Further studies should be performed to globally confirm these findings. ; Fil: Encinas, Micaela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Marfil, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina ; Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina ; Fil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y ...

وصف الملف: application/pdf

العلاقة: info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1472979217304924Test; http://hdl.handle.net/11336/97548Test; Encinas, Micaela; Marfil, Maria Jimena; Garbaccio, Sergio Gabriel; Barandiaran, Soledad; Huertas, Pablo Sebastián; et al.; Mycobacterium bovis ESAT-6, CFP-10 and EspC antigens show high conservation among field isolates; Churchill Livingstone; Tuberculosis (Edinb); 111; 7-2018; 143-146; CONICET Digital; CONICET

الإتاحة: https://doi.org/10.1016/j.tube.2018.06.007Test
http://hdl.handle.net/11336/97548Test