المؤلفون: Muhammad Sasmito Djati, Yuyun Ika Christina, Dinia Rizqi Dwijayanti, Sri Rahayu

المصدر: Veterinary World, Vol 17, Iss 3, Pp 728-734 (2024)

مصطلحات موضوعية: elephantopus scaber, inflammation, macrophage, nitric oxide, proinflammatory cytokines, sauropus androgynous, Animal culture, SF1-1100, Veterinary medicine, SF600-1100

الوصف: Background and Aim: Elephantopus scaber (ES) and Sauropus androgynus (SA) have broad biological effects and have long been used in traditional medicine. However, the anti-inflammatory properties of the combination of ES and SA have not yet been fully explored. This study aimed to investigate the anti-inflammatory activities of the combination of ES and SA ethanol extract on lipopolysaccharide (LPS)-activated RAW 264.7 macrophage cell lines by inhibiting proinflammatory mediators and cytokines. Materials and Methods: Nitric oxide (NO) production in RAW 264.7 cells was assessed using the Griess protocol. The effects of the combination of ES and SA ethanol extract on RAW 264.7 cell viability were determined using WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitro-phenyl)-2H-5-tetrazolio]-1,3-benzene sulfonate) assay. The levels of proinflammatory cytokines, including interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta (IL-β), as well as the production of inducible nitric oxide synthase (iNOS), were assessed using flow cytometry. Results: This study demonstrated that ES and SA have excellent NO, iNOS, and proinflammatory inhibitory activities on LPS-induced RAW 264.7 macrophages. The formula ratio of 2ES:1SA showed the best NO inhibitory activity without any cytotoxicity, whereas the higher dose of SA (1ES:2SA) showed the best suppression of iNOS and proinflammatory cytokines IL-1β, IFN-γ, and TNF-α. Conclusion: The combination of ES and SA ethanol extract could be an alternative agent for reducing excessive inflammation in inflammatory diseases.

وصف الملف: electronic resource

العلاقة: https://www.veterinaryworld.org/Vol.17/March-2024/26.pdfTest; https://doaj.org/toc/0972-8988Test; https://doaj.org/toc/2231-0916Test

الوصول الحر: https://doaj.org/article/e9e5fc9cd9a7414aa9d6beec286a0484Test

المؤلفون: Kharat, Sopan N.^{1, 2}, Mendhulkar, Vijay D.^{2, 1}

المصدر: Research Journal of Pharmacy and Technology 16(12):5834-5843. 2023

المؤلفون: Rui-Hong Gong, Jia-Wen Chen, Li-Sha Shen, Yu-Shan Lin, Haiyang Yu, Sibao Chen, Guo-Qing Chen

المصدر: Journal of Functional Foods, Vol 113, Iss , Pp 106009- (2024)

مصطلحات موضوعية: Elephantopus scaber, Hepatocellular carcinoma, Cell cycle, Apoptosis, Metastasis, PI3K/Akt pathway, Nutrition. Foods and food supply, TX341-641

الوصف: Elephantopus scaber, globally acknowledged for its edible and medicinal uses, remains underexplored despite the identification of active compounds. This research explores Elephantopus scaber extract's therapeutic capabilities in hepatocellular carcinoma (HCC). Initial in vitro screenings using the MTT assay revealed that ethanol extract (EEES) inhibited HCC cell proliferation while exhibiting low toxicity to normal cells. Further explorations showed EEES induced cell cycle arrest, apoptosis, and inhibited cell metastasis. A bioinformatics analysis using a network pharmacology approach identified the PI3K/Akt pathway as a key modulation for EEES's anti-HCC activities, validated through western blot analysis. In animal studies, EEES demonstrated the ability to inhibit HCC tumor growth in vivo with a high level of safety. In summary, our research positions Elephantopus scaber as a potent herb with anti-HCC activities and remarkable safety, offering promising prospects for the prevention and treatment of liver diseases, including HCC.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S1756464624000112Test; https://doaj.org/toc/1756-4646Test

الوصول الحر: https://doaj.org/article/b5c6374bd5694130a45eac499fdecc59Test

المؤلفون: Riski Sulistiarini, Anggi Puranti, Wisnu Cahyo Prabowo

المصدر: Journal of Advanced Biotechnology and Experimental Therapeutics, Vol 6, Iss 2, Pp 436-444 (2023)

مصطلحات موضوعية: hemorrhoids, croton oil, elephantopus scaber, flavonoids, total flavonoid, Biotechnology, TP248.13-248.65

الوصف: Hemorrhoids occur due to inflammation of the rectoanal venous plexus, which causes inflammation, pain, and can cause lumps. This inflammation can cause difficulty in defecation and can even cause heavy bleeding. Empirically Tapak Liman (Elephantopus scaber) leaves have been used to treat hemorrhoids and have been known to contain flavonoids and terpenoid lactones which are active as an anti-inflammatory. Objective: To determine the anti hemorrhoidal activity of E. scaber leaves in reducing inflammation in hemorrhoids in Wistar rats. Material and method: The research begins with the extraction of E. scaber leaves with 70% ethanol as solvent. Extracts were analyzed for total flavonoid content with quercetin standard. Antihemorrhoidal activity was measured based on the degree of edema formed based on the weight of the rectum through the surgical process. Rats were induced with croton oil on the anorectal area for three days. The treatments for the test group were 50, 100, and 200 mg/kgW. The drug control group used oral diclofenac sodium for seven days. Result: E. scaber leaf ethanol extract contains alkaloids, flavonoids, saponins, and polyphenols. The flavonoid level was 3.433mgQE/g extract. This study showed that E. scaber leaf extract had the best anti hemorrhoidal activity at a dose of 100 mg/kgW with a significance value of p [ J Adv Biotechnol Exp Ther 2023; 6(2.000): 436-444]

وصف الملف: electronic resource

العلاقة: http://www.ejmanager.com/fulltextpdf.php?mno=146922Test; https://doaj.org/toc/2616-4760Test

الوصول الحر: https://doaj.org/article/71aa89027ce641ccbfba60d017b11728Test

المؤلفون: Wang, C, Sun, D, Deng, Q, Sun, L, Hu, L, Fang, Z, Zhao, J, Gooneratne, R

المصدر: urn:ISSN:2072-6643 ; Nutrients, 16, 2

مصطلحات موضوعية: Digestive Diseases, 2.1 Biological and endogenous factors, 2 Aetiology, Inflammatory and immune system, Animals, Mice, Gastrointestinal Microbiome, Claudin-1, Serotonin, Polysaccharides, Butyric Acid, Asteraceae, Cytokines, Glutamic Acid, Heat Stress Disorders, Elephantopus scaber L. polysaccharides, gut microbiota, heat stress, neurotransmitters, short chain fatty acids, systemic inflammation, anzsrc-for: 0908 Food Sciences, anzsrc-for: 1111 Nutrition and Dietetics

الوصف: Elephantopus scaber L. (ESL) is a Chinese herb that is used both as a food and medicine, often being added to soups in summer in south China to relieve heat stress (HS), but its exact mechanism of action is unknown. In this study, heat-stressed mice were gavaged with ESL polysaccharides (ESLP) at 0, 150, 300, and 450 mg/kg/d−1 (n = 5) for seven days. The gut microbiota composition, short-chain fatty acids (SCFAs), seven neurotransmitters in faeces, expression of intestinal epithelial tight junction (TJ) proteins (Claudin-1, Occludin), and serum inflammatory cytokines were measured. The low dose of ESLP (ESLL) improved the adverse physiological conditions; significantly reduced the cytokines (TNF-α, IL-1β, IL-6) and lipopolysaccharide (LPS) levels (p < 0.05); upregulated the expression of Claudin-1; restored the gut microbiota composition including Achromobacter and Oscillospira, which were at similar levels to those in the normal control group; significantly increased beneficial SCFAs like butyric acid and 5-HT levels in the faeces of heat-stressed mice; and significantly decreased the valeric acid and glutamic acid level. The level of inflammatory markers significantly correlated with the above-mentioned indicators (p < 0.05). Thus, ESLL reduced the HS-induced systemic inflammation by optimizing gut microbiota (Achromobacter, Oscillospira) abundance, increasing gut beneficial SCFAs like butyric acid and 5-HT levels, and reducing gut valeric and glutamic acid levels.

وصف الملف: application/pdf

العلاقة: http://hdl.handle.net/1959.4/unsworks_85596Test; https://unsworks.unsw.edu.au/bitstreams/53bc1e02-880b-4d41-8bbc-c68900552080/downloadTest; https://doi.org/10.3390/nu16020262Test

الإتاحة: https://doi.org/10.3390/nu16020262Test
http://hdl.handle.net/1959.4/unsworks_85596Test
https://unsworks.unsw.edu.au/bitstreams/53bc1e02-880b-4d41-8bbc-c68900552080/downloadTest

المؤلفون: Chen Wang, Dongfang Sun, Qi Deng, Lijun Sun, Lianhua Hu, Zhijia Fang, Jian Zhao, Ravi Gooneratne

المصدر: Nutrients, Vol 16, Iss 2, p 262 (2024)

مصطلحات موضوعية: Elephantopus scaber L. polysaccharides, heat stress, systemic inflammation, gut microbiota, short chain fatty acids, neurotransmitters, Nutrition. Foods and food supply, TX341-641

الوصف: Elephantopus scaber L. (ESL) is a Chinese herb that is used both as a food and medicine, often being added to soups in summer in south China to relieve heat stress (HS), but its exact mechanism of action is unknown. In this study, heat-stressed mice were gavaged with ESL polysaccharides (ESLP) at 0, 150, 300, and 450 mg/kg/d −1 ( n = 5) for seven days. The gut microbiota composition, short-chain fatty acids (SCFAs), seven neurotransmitters in faeces, expression of intestinal epithelial tight junction (TJ) proteins (Claudin-1, Occludin), and serum inflammatory cytokines were measured. The low dose of ESLP (ESLL) improved the adverse physiological conditions; significantly reduced the cytokines (TNF-α, IL-1β, IL-6) and lipopolysaccharide (LPS) levels ( p < 0.05); upregulated the expression of Claudin-1; restored the gut microbiota composition including Achromobacter and Oscillospira , which were at similar levels to those in the normal control group; significantly increased beneficial SCFAs like butyric acid and 5-HT levels in the faeces of heat-stressed mice; and significantly decreased the valeric acid and glutamic acid level. The level of inflammatory markers significantly correlated with the above-mentioned indicators ( p < 0.05). Thus, ESLL reduced the HS-induced systemic inflammation by optimizing gut microbiota ( Achromobacter , Oscillospira ) abundance, increasing gut beneficial SCFAs like butyric acid and 5-HT levels, and reducing gut valeric and glutamic acid levels.

العلاقة: https://www.mdpi.com/2072-6643/16/2/262Test; https://doaj.org/toc/2072-6643Test; https://doaj.org/article/e39acdb7a66a4195b7897b9e7a578ac4Test

الإتاحة: https://doi.org/10.3390/nu16020262Test
https://doaj.org/article/e39acdb7a66a4195b7897b9e7a578ac4Test

المؤلفون: Muhammad Sasmito Djati, Yuyun Ika Christina, Muhaimin Rifa'i

المصدر: Veterinary World, Vol 14, Iss 5, Pp 1398-1404 (2021)

مصطلحات موضوعية: elephantopus scaber, erythropoiesis, follicle-stimulating hormone, luteinizing hormone, sauropus androgynus, Animal culture, SF1-1100, Veterinary medicine, SF600-1100

الوصف: Background and Aim: Escherichia coli infection produces an adverse effect on the erythrocyte lineage and hormone levels during pregnancy. This study aimed to evaluate the effects of Elephantopus scaber (ES) and Sauropus androgynus (SA) in combination on circulating follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels and erythropoiesis changes in E. coli-infected pregnant mice. Materials and Methods: Female Balb/c mice were mated with normal male mice and pregnancies were identified by the formation of vaginal plugs. Twenty-eight pregnant mice were divided randomly into seven groups: A control group (N), E. coli-infected pregnant mice (K+), and infected pregnant mice received the following five treatments: (1) Only ES; (2) ESSA1 (75:25); (3) ESSA2 (50:50); (4) ESSA3 (25:75); and (5) only SA, beginning from the 1st to the 16th day of pregnancy. Pregnant mice were infected with 107 CFU/mL of E. coli on day 4. Blood serum was collected on days 8, 12, and 16 of pregnancy and LH and FSH levels were measured by enzyme-linked immunosorbent assay. Bone marrow was isolated to determine the relative number of TER-119+VLA4+ and TER-119+CD34+ using flow cytometry. Results: The ESSA1 and SA groups exhibited a marked increase in LH levels. The combination of ES and SA administered at a 25:75 ratio (ESSA3) altered FSH levels and the relative number of TER-119+VLA4+ in infected pregnant mice. Combined with SA at an equal ratio (50:50), ESSA2 group exhibited a significant increase in the expression of TER119+CD34+ compared with the other treatment groups. Conclusion: ES and SA combined at a ratio of 25:75 exhibited optimal results in altering hormonal and erythropoiesis in infected pregnant mice.

وصف الملف: electronic resource

العلاقة: http://www.veterinaryworld.org/Vol.14/May-2021/43.pdfTest; https://doaj.org/toc/0972-8988Test; https://doaj.org/toc/2231-0916Test

الوصول الحر: https://doaj.org/article/bbe3a0e1416944d3bf9b083966568cadTest

المؤلفون: Yuyun Ika Christina, Meyla Restia Diana, Eka Noviya Fuzianingsih, Nurhayati, Faradisa Noviani Ridwan, Widodo, Muhaimin Rifa’i, Muhammad Sasmito Djati

المصدر: Journal of Ayurveda and Integrative Medicine, Vol 12, Iss 2, Pp 245-253 (2021)

مصطلحات موضوعية: E. coli, Elephantopus scaber, Hormonal, Pregnant, Sauropus androgynous, Miscellaneous systems and treatments, RZ409.7-999

الوصف: Background: Elephantopus scaber (ES) and Sauropus androgynous (SA) have been frequently reported to possess antibacterial activity through in vitro, but in vivo studies about the protective effect of combined ES and SA have acquired less attention. Objectives: To evaluate protective effect of combined ethanol extract of ES and SA on hormone imbalance and renal and hepatic necrosis formation in Escherichia coli-infected pregnant mice. Materials and methods: A total of 28 pregnant Balb/c mice were divided into seven groups (n = 4): control, E. coli-infected pregnant mice, infected pregnant mice received 200 mg/kg ES, infected pregnant mice received combined 150 mg/kg ES and 37.5 mg/kg SA (75:25), 100 mg/kg ES and 75 mg/kg SA (50:50), 50 mg/kg ES and 112.5 mg/kg SA (25:75), and only 150 mg/kg SA. Pregnant mice were orally treated with combined ES and SA on day 1–4th of pregnancy. On the 4th day, mice were infected with 107 CFU/mL of E. coli and continuously treated with ES and SA until the 16th day of pregnancy. After treatment, the kidney and liver were prepared for histological examination using H&E staining. The blood serum was collected in each stage of pregnancy and measured by ELISA assays. Results: Combined ES and SA gave an impact on altering the prolactin level. Combined ES and SA at ratio dose 75:25 was able to restore progesterone to normal levels (P

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S097594762030084XTest; https://doaj.org/toc/0975-9476Test

الوصول الحر: https://doaj.org/article/c9556c58a0b5472990aed181eb307f9fTest

المؤلفون: Yuanhui Gao, Zhenyu Nie, Hui Cao, Denggao Huang, Mei Chen, Yang Xiang, Xiaolong Yu, Shufang Zhang

المصدر: Cancers, Vol 14, Iss 23, p 5976 (2022)

مصطلحات موضوعية: scabertopin, bladder cancer, reactive oxygen species, necroptosis, Elephantopus scaber L., Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Bladder cancer remains one of the most common malignant tumors that threatens human health worldwide. It imposes a heavy burden on patients and society due to the high medical costs associated with its easy metastasis and recurrence. Although several treatment options for bladder cancer are available, their clinical efficacy remains unsatisfactory. Therefore, actively exploring new drugs and their mechanisms of action for the clinical treatment of bladder cancer is very important. Scabertopin is one of the major sesquiterpene lactones found in Elephantopus scaber L. Sesquiterpene lactones are thought to have fairly strong anti-cancer efficacy. However, the anticancer effect of sesquiterpenoid scabertopin on bladder cancer and its mechanism are still unclear. The aim of this study is to evaluate the antitumor activity of scabertopin in bladder cancer and its potential molecular mechanism in vitro. Our results suggest that scabertopin can induce RIP1/RIP3-dependent necroptosis in bladder cancer cells by promoting the production of mitochondrial reactive oxygen species (ROS), inhibit the expression of MMP-9 by inhibiting the FAK/PI3K/Akt signaling pathway, and ultimately inhibit the migration and invasion ability of bladder cancer cells. At the same time, we also demonstrated that the half-inhibition concentration (IC50) of scabertopin on various bladder cancer cell lines (J82, T24, RT4 and 5637) is much lower than that on human ureteral epithelial immortalized cells (SV-HUC-1). The above observations indicate that scabertopin is a potential therapeutic agent for bladder cancer that acts by inducing necroptosis and inhibiting metastasis.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2072-6694/14/23/5976Test; https://doaj.org/toc/2072-6694Test

الوصول الحر: https://doaj.org/article/b3e0f767f14740ca80b78029aa222939Test

المؤلفون: Tri Agusti Sholikah, Sri Wulandari, Indah Ariesta, Muhammad Arif Rakhman Hakim, Muhammad Hafizhan

المصدر: JKKI (Jurnal Kedokteran dan Kesehatan Indonesia), Pp 172-179 (2020)

مصطلحات موضوعية: elephantopus scaber l, flavanoid, dm rat model, Medicine

الوصف: Background: Diabetes mellitus (DM) is among the diseases with high morbidity and mortality The pathogenesis of DM involves pancreatic ß-cell damage or insulin sensitivity disorder that result in hyperglycemia. Tapak liman (Elephantopus scaber L) is known to have high flavonoid content. Flavonoids are antioxidants that play a role in reducing pancreatic ß-cell damage or damage to other tissues, which potentially reduce blood glucose levels. Objective: To determine the hypoglycemic effects of tapak liman using the DM rat models. Methods: Twenty-eight Wistar albino rats (Rattus novergicus) were divided into four groups: the normal control group (KKn), not induced by streptozotocin-nicotinamide (STZ-NA) intraperitoneal; negative control group (KK-), induced by STZ-NA; treatment group 1 (KP1), induced by STZ-NA and 150 mg/kg body weight of tapak liman plant extract; and the treatment group 2 (KP2), induced by STZ-NA and 300 mg/kg body weight of tapak liman. Blood glucose levels were measured on the 7th day after STZ-NA induction and the 28th day after the administration of tapak liman plant extract. The results were analyzed for statistical significance. Results: There was a significant decrease in blood glucose levels in KP1 and KP2 (p= 0.05). The decrease in blood glucose in KP1 had not reached normal levels compared to KP2. Conclusion: Administration of tapak liman plant extract at a dose of 300 mg/kg body weight in DM rat models reduced blood glucose levels to normal.

وصف الملف: electronic resource

العلاقة: https://journal.uii.ac.id/JKKI/article/view/13115Test; https://doaj.org/toc/2085-4145Test; https://doaj.org/toc/2527-2950Test

الوصول الحر: https://doaj.org/article/a641db96fae84316b717559942ab67bfTest