المؤلفون: Rossignol, Jean-Michel, Abergel, Chantal, Capy, Pierre, Claverie, Jean-Michel, Vienne, Dominique de, Ehrhart, Jean-Claude, Forterre, Patrick, Feunteun, Jean, Nicolas, Jean-François

مصطلحات موضوعية: Glossaire, biologie, Glossary, biology

الوصف: A Activation de la transcription Induction de la transcription* d’un gène* (cf. Répression). La régulation de la transcription peut avoir lieu : en cis. C’est un effet positionnel dû à l’insertion à proximité du gène d’une séquence (cf. Cis Regulatory Element) capable d’augmenter sa transcription (promoteur-activateur). en trans. Dans ce cas c’est un facteur protéique qui intervient directement sur la machinerie de transcription du gène (transactivateur). Activation d’un proto-oncogène en on.

العلاقة: info:eu-repo/semantics/reference/issn/2260-3875; info:eu-repo/semantics/reference/issn/2265-786X; urn:doi:10.4000/hrc.7798; https://journals.openedition.org/hrc/7798Test

الإتاحة: https://doi.org/10.4000/hrc.7798Test
https://journals.openedition.org/hrc/7798Test

المؤلفون: Ehrhart, Jean-Claude

الوصف: Th.--Sci. nat.--Paris 11--Orsay, 1978. N°: 1969.
Extr. en partie de Biochimica et biophysica acta, 375, 1975, 434-445 ; de Biochimie, 57, 8, 1975, 987-989 et de F.E.B.S. Letters, 78, 2, 1977, 295-299.

الإتاحة: http://catalogue.bnf.fr/ark:/12148/cb36073960qTest

المؤلفون: Breton, François, Bennetau, Bernard, Lidereau, Rosette, Thomas, Laurent, Regnier, Gilles, Ehrhart, Jean-Claude, Tauc, Patrick, Tran, Phuong-Lan

المساهمون: Institut d'Alembert (IDA), École normale supérieure - Cachan (ENS Cachan)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de chimie organique et organométallique (LCOO), Université Sciences et Technologies - Bordeaux 1 (UB)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Genetique Moleculaire des Cancers d'Origine Epitheliale, Institut Curie Paris -Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Procédés et Ingénierie en Mécanique et Matériaux (PIMM), Conservatoire National des Arts et Métiers CNAM (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre National de la Recherche Scientifique (CNRS)-Arts et Métiers Sciences et Technologies, HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), Stabilité Génétique et Oncogenèse (UMR 8200), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie et de Pharmacologie Appliquée (LBPA)

المصدر: ISSN: 1387-2176.

مصطلحات موضوعية: Breast cancer, Circulating tumor cells (CTCs), Targeted cell capture, Immunosensor, Multiplex device, Long alkylsilane monolayer, [SPI.MAT]Engineering Sciences [physics]/Materials

الوصف: International audience ; We have recently reported the analytical performance of an immunosensor comprising one mm-scale parallel plate laminar flow chamber and applied to capture MCF7 breast cancer cells (Ehrhart et al., Biosens. Bioelectr. 24, 467, 2008). Herein we present a new multiplex immunosensor embodying four parallel plate laminar flow chambers that fit onto a standard, functionalized, microscopy glass slide. The four surfaces are coated with long alkyl chain spacers of 21-aminohenicosyl trichlorosilane (AHTS) and then grafted with a monoclonal anti-human epithelial cell adhesion molecule (EpCAM) antibody specific of target cells to immobilize. We first demonstrate a significantly (P < 0.01) improved capacity of each of the four flow chambers of the multiplex immunosensor to capture MCF7 cells compared to the previous single chamber device. Second, in addition to an increase of cell immobilization, the multiplex device offers a versatile tool easily grafted with various purified antibodies onto the four surfaces. Third, we obtained high cell capture rate and efficiency of various numbers of MCF7 cells spiked in buffer containing an equal number of background leukocytes. And fourth, we demonstrate isolation efficiency of circulating tumor cells (CTCs) from peripheral blood drawn from a small cohort of patients with localized or metastatic breast cancer. This new multiplex immunosensor could be tested for its potential to capture different subpopulations of CTCs.

العلاقة: hal-02428520; https://hal.science/hal-02428520Test; https://hal.science/hal-02428520/documentTest; https://hal.science/hal-02428520/file/PIMM_BM_2011_REGNIER.pdfTest

الإتاحة: https://doi.org/10.1007/s10544-010-9465-7Test
https://hal.science/hal-02428520Test
https://hal.science/hal-02428520/documentTest
https://hal.science/hal-02428520/file/PIMM_BM_2011_REGNIER.pdfTest

المؤلفون: Abergel, Chantal, Capy, Pierre, Claverie, Jean-Michel, Crespy, Cécile, Ehrhart, Jean-Claude, Feunteun, Jean, Forterre, Patrick, Fournier, Patrick, Harter, Hélène, Jacques, Guy, Nicolas, Jean-François, Recondo, Anne-Marie de, Rossignol, Jean-Michel, Vienne, Dominique de, Weill, Alain

المساهمون: Rossignol, Jean-Michel

مصطلحات موضوعية: scipo, hisphilso

العلاقة: urn:doi:10.4000/hrc.7639; https://journals.openedition.org/hrc/7639Test

الإتاحة: https://doi.org/10.4000/hrc.7639Test
https://journals.openedition.org/hrc/7639Test

المؤلفون: Bastien, Nathalie, Becerril, Cecilia, Bespalov, Vyacheslav A, Boiteux, Serge, Boszko, Ihor P, Brash, Douglas E, Cadet, Jean, Caney, Colleen, Cheung Jr, K-John, Conconi, Antonio, Culerrier, Raphael M, David, Jocelyn, Davies, R Jeremy H, Desnoyers, Julie, Douki, Thierry, Drobetsky, Elliot, Ehrhart, Jean-Claude, Fahy, Deirdre, Francis, Murray A, Gosselet, Fabien P, Grof, Pal, Lee, Dong-Hyun, Leger, Caroline, Li, Gang, Mathonnet, Geraldine, McKay, Bruce C, Mullenders, Leon H F, Oh, Dennis H, Perdiz, Daniel, Pfeifer, Gerd P, Pitsikas, Photini, Pouget, Jean-Pierre, Rainbow, Andrew J, Ramotar, Dindial, Ravanat, Jean-Luc, Reynaud-Angelin, Anne, Rochette, Patrick, Sarasin, Alain, Shah, Girish M Shah, Smerdon, Michael J, Spronck, Jennifer, Starrs, Sharon M, Stevenson, Clarke S, Taylor, John-Stephen, Vodenicharov, Momchil D

المساهمون: Sage, Evelyne, Drouin, Regen, Rouabhia, Mahmoud, Hader, Donat-P, Jori, Giulio

المصدر: ISBN 9780854043262 9780854043262 9781847552501.

الوصف: As modern day society takes an increasing interest in outdoor activities, its exposure to sunlight has never been greater. As a consequence, countries throughout the world are experiencing a dramatic increase in the incidences of skin carcinomas and melanomas. From DNA photolesions to mutations, skin cancer and cell death provides an authoritative source of information for photobiologists interested in the series of genetic events that occur in the skin, and eventually lead to cancer. With contributions from eminent scientists in the field, this book includes the latest information on DNA photolesions and repair, as well as the key mechanisms of solar UV in skin cancer initiation and development. Significant information relating to UV-induced photolesions and mechanisms of skin tumour occurrence is also included. By providing the basic phenomena underlying the science and an overview of the biological events that take place when cells are exposed to solar UV radiation, From DNA photolesions to mutations, skin cancer and cell death is suitable to all researchers interested in the process of photocarcinogenesis.

الإتاحة: https://doi.org/10.1039/9781847552501Test

المؤلفون: Breton, François, Bennetau, Bernard, Lidereau, Rosette, Thomas, Laurent, Regnier, Gilles, Ehrhart, Jean-Claude, Tauc, Patrick, Tran, Phuong-Lan

المصدر: Biomedical Microdevices ; volume 13, issue 1, page 1-9 ; ISSN 1387-2176 1572-8781

مصطلحات موضوعية: Molecular Biology, Biomedical Engineering

الإتاحة: https://doi.org/10.1007/s10544-010-9465-7Test

المؤلفون: Ehrhart, Jean-Claude, Bennetau, Bernard, Renaud, Louis, Madrange, Jean-Pierre, Thomas, Laurent, Morisot, Julie, Brosseau, Arnaud, Allano, Sylvain, Tauc, Patrick, Tran, Phuong-Lan

المساهمون: Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre de recherche cerveau et cognition (CERCO), Institut des sciences du cerveau de Toulouse. (ISCT), Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Department of Physics, University of Wales, Systèmes et Applications des Technologies de l'Information et de l'Energie (SATIE), École normale supérieure - Cachan (ENS Cachan)-Université Paris-Sud - Paris 11 (UP11)-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-École normale supérieure - Rennes (ENS Rennes)-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Conservatoire National des Arts et Métiers CNAM (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Photophysique et Photochimie Supramoléculaires et Macromoléculaires (PPSM), École normale supérieure - Cachan (ENS Cachan)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie et de Pharmacologie Appliquée (LBPA), École normale supérieure - Cachan (ENS Cachan)-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 0956-5663 ; Biosensors and Bioelectronics ; https://hal.science/hal-00421165Test ; Biosensors and Bioelectronics, 2008, 24 (3), pp.467-74. ⟨10.1016/j.bios.2008.04.027⟩.

مصطلحات موضوعية: MESH: Antibodies, Monoclonal, MESH: Antigen-Antibody Reactions, MESH: Antigens, Neoplasm, MESH: Biosensing Techniques, MESH: Breast Neoplasms, MESH: Cell Adhesion Molecules, MESH: Cell Line, Tumor, MESH: Female, MESH: Humans, MESH: Silanes, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics

الوصف: International audience ; We designed a new efficient and reliable immunosensor and demonstrated its analytic performance to capture breast cancer MCF7 and T47D cells, under laminar flow, onto antibody-coated long alkylsilane self-assembled monolayers (SAMs) in a parallel plate flow chamber. The surface floor of the laminar flow chamber was grafted with an amino-terminated long alkyl chain spacer, 21-aminohenicosyl trichlorosilane (AHTS) followed by tethering a specific monoclonal antibody directed against the human epithelial cell adhesion molecule (EpCAM) antigen, which is overexpressed in primary breast cancer. Properties of the AHTS- and antibody-grafted surface floor were compared to that of surface floors coated with the short alkyl spacers 3-glycidoxy-propyl trimethoxysilane (GPTS) or 3-aminopropyl triethoxysilane (APTES) and antibodies. A theoretical model was constructed according to the geometry of the flow chamber in order to calculate the trajectories that would use cell flows. Cell capture experiments demonstrated that cell immobilization was optimized throughout the whole flow chamber. High cell capture was yielded on antibody-tethered long alkyl AHTS surface. This new procedure offers multiple advantages: a versatile tool readily applied to a panel of purified antibodies, an enrichment of cell immobilization using repetitive cell flow, and a stable capturing surface suitable for long term storage and handling.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/18547800; hal-00421165; https://hal.science/hal-00421165Test; PUBMED: 18547800

الإتاحة: https://doi.org/10.1016/j.bios.2008.04.027Test
https://hal.science/hal-00421165Test

المؤلفون: Gosselet, Fabien P., Magnaldo, Thierry, Culerrier, Raphaël M., Sarasin, Alain, Ehrhart, Jean-Claude

المصدر: Cellular Signalling ; volume 19, issue 4, page 731-739 ; ISSN 0898-6568

مصطلحات موضوعية: Cell Biology

الإتاحة: https://doi.org/10.1016/j.cellsig.2006.09.006Test
https://api.elsevier.com/content/article/PII:S0898656806002658?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0898656806002658?httpAccept=text/plainTest

المؤلفون: Ehrhart, Jean-Claude, Gosselet, Fabien P., Culerrier, Raphaël M., Sarasin, Alain

المصدر: Photochemical & Photobiological Sciences ; volume 2, issue 8, page 825-834 ; ISSN 1474-905X 1474-9092

مصطلحات موضوعية: Physical and Theoretical Chemistry

الإتاحة: https://doi.org/10.1039/b302281aTest
http://pubs.rsc.org/en/content/articlepdf/2003/PP/B302281ATest

المؤلفون: Ehrhart, Jean-Claude, Chauveau, Jean

المصدر: FEBS Letters ; volume 78, issue 2, page 295-299 ; ISSN 0014-5793 1873-3468

الإتاحة: https://doi.org/10.1016/0014-5793Test(77)80327-x