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1دورية أكاديمية
المؤلفون: Ahmed Bassiouni, Edward John Cleland, Alkis James Psaltis, Sarah Vreugde, Peter-John Wormald
المصدر: PLoS ONE, Vol 10, Iss 4, p e0123216 (2015)
الوصف: BACKGROUND:The role of the sino-nasal microbiome in CRS remains unclear. We hypothesized that the bacteria within mucosal-associated biofilms may be different from the more superficial-lying, free-floating bacteria in the sinuses and that this may impact on the microbiome results obtained. This study investigates whether there is a significant difference in the microbiota of a sinonasal mucosal tissue sample versus a swab sample. METHODS:Cross-sectional study with paired design. Mucosal biopsy and swab samples were obtained intra-operatively from the ethmoid sinuses of 6 patients with CRS. Extracted DNA was sequenced on a Roche-454 sequencer using 16S-rRNA gene targeted primers. Data were analyzed using QIIME 1.8 software package. RESULTS:At a maximum subsampling depth of 1,100 reads, the mean observed species richness was 33.3 species (30.6 for swab, versus 36 for mucosa; p > 0.05). There was no significant difference in phylogenetic and non-phylogenetic alpha diversity metrics (Faith's PD_Whole_Tree and Shannon's index) between the two sampling methods (p > 0.05). The type of sample also had no significant effect on phylogenetic and non-phylogenetic beta diversity metrics (Unifrac and Bray-Curtis; p > 0.05). CONCLUSION:We observed no significant difference between the microbiota of mucosal tissue and swab samples. This suggests that less invasive swab samples are representative of the sinonasal mucosa microbiome and can be used for future sinonasal microbiome studies.
وصف الملف: electronic resource
العلاقة: http://europepmc.org/articles/PMC4396979?pdf=renderTest; https://doaj.org/toc/1932-6203Test
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المصدر: American Journal of Rhinology & Allergy. 30:37-43
مصطلحات موضوعية: Male, 0301 basic medicine, Pathology, medicine.medical_specialty, Chronic rhinosinusitis, Inflammatory response, Treatment outcome, 03 medical and health sciences, 0302 clinical medicine, Paranasal Sinuses, Staphylococcus epidermidis, Humans, Immunology and Allergy, Medicine, Postoperative Period, Propionibacterium acnes, Microbiome, Sinusitis, 030223 otorhinolaryngology, Rhinitis, Acinetobacter, business.industry, Microbiota, Endoscopy, Biodiversity, General Medicine, medicine.disease, Treatment Outcome, 030104 developmental biology, Chronic disease, Otorhinolaryngology, Chronic Disease, Immunology, Female, Species richness, business
الوصف: Background The bacterial microbiome in chronic rhinosinusitis (CRS) remains poorly understood. Microorganisms are believed to be important contributors to the inflammatory response seen in these patients. Objective To examine the bacterial CRS microbiome by using a pyrosequencing technique and determine the diversity, richness, prevalence, and abundance of bacterial species in these patients. Furthermore, the postoperative changes that occur in the microbiome and correlations with patient outcomes are assessed. Methods Swabs were collected from 23 patients with CRS and 11 controls during surgery. Further postoperative swabs were collected in the CRS group. Bacterial DNA was extracted from the swabs and then sequenced by using 16S ribosomal DNA bacterial tag-encoded FLX amplicon pyrosequencing. Results A total of 456 unique bacterial species were detected. No difference was seen for richness or diversity between the study groups (p > 0.05). Diversity declined after surgery in the CRS group (p = 0.01). Propionibacterium acnes and Staphylococcus epidermidis were the most prevalent species. Several significant differences were determined for prevalence and mean relative abundance (MRA) between the study groups. In particular, Acinetobacter johnsonii was more prevalent and had a higher MRA in the controls. Furthermore, the MRA of this species increased after surgery and was associated with improved quality of life. Conclusion This study characterized the sinonasal microbiome in a group of controls and patients with CRS. Important differences in diversity, prevalence, abundance, and temporal changes were described. Of great interest is the potential association between A. johnsonii and health. These findings provide new insights into the interplay between the microbiome and health in the paranasal sinuses.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ab3a1b3e7d48a34de4808bcde760d73Test
https://doi.org/10.2500/ajra.2016.30.4261Test -
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المؤلفون: Sarah Vreugde, Samuel Boase, Peter-John Wormald, Edward John Cleland, Scot E. Dowd, Ahmed Bassiouni
المصدر: International Forum of Allergy & Rhinology. 4:259-265
مصطلحات موضوعية: Fusarium, Pathology, medicine.medical_specialty, biology, Chronic rhinosinusitis, Disease, biology.organism_classification, Gastroenterology, Otorhinolaryngology, Internal medicine, medicine, Immunology and Allergy, Pyrosequencing, Species richness, Malassezia, Microbiome, Ribosomal DNA
الوصف: Background Our understanding of fungi in chronic rhinosinusitis (CRS) has been limited by previously employed detection techniques. This study examines the fungal component of the microbiome in CRS patients and controls using a highly sensitive culture-independent molecular technique. The aims of this study include the characterization of fungal richness, prevalence, abundance, temporal changes, and their relationship with patient outcomes. Methods Swabs were collected from the sinuses of 23 CRS patients and 11 controls. Collection occurred intraoperatively, and at 6 and 12 weeks postoperatively. DNA was extracted from the swabs and fungal outcomes were determined through 18S ribosomal DNA (rDNA) fungal tag-encoded FLX amplicon pyrosequencing. Results Fungi were ubiquitous to all patients. A total of 207 fungal genera were detected, with a mean sample richness of 8.18 and 12.14 in the control and CRS groups, respectively. Malassezia was detected in all patients at surgery and was also the most abundant. Postoperatively, fungal richness decreased (p < 0.05) and was associated with declines in the prevalence of Fusarium and Neocosmospora (p < 0.05). Neocosmospora was also less abundant postoperatively (p < 0.05). No correlations were found with quality of life. Conclusion This is the first study to use a highly sensitive pyrosequencing technique to reveal the true diversity of fungi in the sinuses of CRS patients and postoperative changes in richness. The presence of Malassezia, a genus not previously described in the sinuses, is of great interest, and its potential as a disease modifier should see further investigation given its association with atopic disease.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::ed6af79a88aa661759e8ec8723b6bd3dTest
https://doi.org/10.1002/alr.21297Test -
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المؤلفون: Camille Jardeleza, Edward John Cleland, Samuel Boase, Peter-John Wormald, Peter Speck, Sandra Morales, Neil Cheng-Wen Tan, Joshua Jervis-Bardy, Amanda Drilling, Sarah Vreugde, Craig James
المصدر: International Forum of Allergy & Rhinology. 4:176-186
مصطلحات موضوعية: Rhinology, medicine.medical_specialty, business.industry, medicine.medical_treatment, Biofilm, Histology, medicine.disease, medicine.disease_cause, Microbiology, Staining, Otorhinolaryngology, In vivo, Staphylococcus aureus, Immunology and Allergy, Medicine, business, Sinusitis, Saline
الوصف: Background Treatment of sinonasal bacterial biofilms continues to be a challenge in modern rhinology. This study's objective was to assess the safety and efficacy of topically applied Cocktail of S. aureus specific phage (CTSA) alone and in combination with ethylenediaminetetraacetic acid (EDTA) for treatment of Staphylococcus aureus biofilms in vivo. Methods Using a sheep model of sinusitis, frontal sinuses (n = 6 per treatment) were flushed once daily with a CTSA (2 × 106 plaque forming units [PFU]/mL), with or without EDTA (0.075 mg/mL), and compared to a control flush containing saline and heat-inactivated CTSA. Safety was assessed using histology and scanning electron microscopy (SEM) after treatment for 3 days. Efficacy was assessed by quantifying the generation of S. aureus biofilms in the frontal sinuses after 5 days of treatment. Biofilm mass was compared between treatment groups and controls using LIVE/DEAD BacLight staining and confocal scanning laser microscopy to visualize the tissue sections. COMSTAT2 software was used to compute the biofilm mass present on tissue sections. Results Tissue morphology was conserved, with no significant signs of inflammation, when comparing control and test treatments. Furthermore, SEM analysis indicated test treatments were not toxic or damaging to mucosal cilia. COMSTAT2 quantification of biofilm showed a significant reduction in biofilm levels when comparing the control with CTSA (p = 0.0043), EDTA (p = 0.0095), and CTSA-EDTA (p = 0.0022) treatments. Conclusion Results indicate that CTSA and EDTA are safe and efficacious for short-term topical application against S. aureus infection in a sheep sinusitis model, and have the potential to be translated to a clinical setting.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::be5b74611abde5b7f1c8312f78ff8b08Test
https://doi.org/10.1002/alr.21270Test -
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المؤلفون: Ahmed Bassiouni, Sarah Vreugde, Edward John Cleland, Craig James, Amanda Drilling, Peter-John Wormald
المصدر: International Forum of Allergy & Rhinology. 4:309-314
مصطلحات موضوعية: Goblet cell, biology, business.industry, Pathogenic bacteria, medicine.disease, medicine.disease_cause, biology.organism_classification, law.invention, Microbiology, Probiotic, medicine.anatomical_structure, Otorhinolaryngology, law, Staphylococcus epidermidis, Staphylococcus aureus, Immunology, medicine, Immunology and Allergy, Nasal administration, Microbiome, Sinusitis, business
الوصف: Background Staphylococcus aureus (SA) is a key pathogenic component of the chronic rhinosinusitis (CRS) microbiome and is associated with increased disease severity and poor postoperative outcomes. Probiotic treatments potentially offer a novel approach to the management of pathogenic bacteria in these recalcitrant patients through supporting a healthy community of commensal species. This study aims to investigate the probiotic properties of Staphylococcus epidermidis (SE) against SA in a mouse model of sinusitis. Methods Twenty C57/BL6 mice received intranasal inoculations of phosphate buffered saline (PBS), SE, SA, or a combination of SE and SA (SE+SA) for 3 days. Following euthanasia, the mouse snouts were harvested and prepared for histological analysis. Counts of periodic acid–Schiff (PAS)-positive goblet cells were the primary outcome measure. Results Goblet cell counts were significantly higher in both the SA and SE+SA groups compared to those receiving PBS or SE alone (p 0.05). The presence of SA postinoculation was confirmed by culture in both the SA and SE+SA groups. Conclusion This study confirms the probiotic potential of SE against SA in a mouse model of sinusitis. Although the interactions that occur between many probiotic species and pathogens are yet to be fully understood, studies such as this support further exploration of ecologically-based treatment paradigms for the management of CRS.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::f19784cb6896d5134d227ba75c9b04f0Test
https://doi.org/10.1002/alr.21279Test -
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المؤلفون: Edward John Cleland, Peter-John Wormald, Ahmed Bassiouni
المصدر: International Forum of Allergy & Rhinology. 3:642-646
مصطلحات موضوعية: medicine.medical_specialty, biology, Pseudomonas aeruginosa, business.industry, Retrospective cohort study, biology.organism_classification, medicine.disease_cause, medicine.disease, Surgery, Pneumonia, Otorhinolaryngology, Staphylococcus aureus, Internal medicine, Haemophilus, Cohort, medicine, Bacteriology, Immunology and Allergy, business, Asthma
الوصف: Background The role of bacteria in the etiopathogenesis of chronic rhinosinusitis (CRS) remains an area of interest. The impact of surgery and factors such as the presence of polyps, asthma, and aspirin sensitivity on the bacterial state are poorly understood. To determine the effect of these factors, this study examines the culture results from a large cohort of CRS patients. Methods This retrospective study used the culture results from 513 CRS patients, which were analyzed for species growth and compared to factors such as previous surgery, presence of polyps, aspirin sensitivity, and asthma. Univariate and multivariate logistic regression models were used for statistical analysis. Results Eighty-three percent (83%) of patients had a positive culture result. The average number of isolates detected per patient was 0.95. S. aureus was the most frequently cultured organism (35%), followed by P. aeruginosa (9%), Haemophilus spp. (7%), and S. pneumonia (5%). Revision patients were more likely to grow S. aureus (p = 0.001), P. aeruginosa (p = 0.044) and have a positive culture (p = 0.001). Asthma was correlated with a positive culture (p = 0.039). No difference was determined between polyp and nonpolyp patients for any of the bacterial outcomes. Conclusion This study highlights important factors in the bacteriology of CRS patients. S. aureus was the most prevalent species identified in our cohort, followed by P. aeruginosa. S. aureus rates of isolation were also significantly higher in patients undergoing revision surgery. No association was found between the presence of nasal polyposis and culture rates.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::01f1009480cc416182e085e02e98d01eTest
https://doi.org/10.1002/alr.21159Test -
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المؤلفون: Peter-John Wormald, Lorwai Tan, Joshua Jervis-Bardy, Sam Boase, Edward John Cleland, Harshita Pant
المصدر: International Forum of Allergy & Rhinology. 3:341-348
مصطلحات موضوعية: biology, Toxin, Biofilm, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, medicine.disease, medicine.disease_cause, Aspergillus fumigatus, Haemophilus influenzae, Microbiology, Fungal sinusitis, Epithelial Damage, Otorhinolaryngology, medicine, Immunology and Allergy, Sinusitis, Bacteria
الوصف: Background Fungal biofilms have been discovered in chronic rhinosinusitis (CRS) patients, but factors contributing to their establishment are obscure. A recent animal study showed bacterial co-inoculation was required. We examine the role of 4 bacterial species and a cilia toxin on fungal biofilm formation in a sheep sinusitis model. The importance of epithelial integrity on fungal biofilm formation is also examined. Methods Forty-eight frontal sinuses were inoculated with Aspergillus fumigatus alone, with 1 of 4 bacteria, or a cilia toxin. Bacterial and fungal biofilm was determined using confocal scanning laser microscopy. Inflammation and cilia integrity were assessed using light microscopy and transmission electron microscopy, respectively. Results No fungal biofilm formed when inoculated alone. Florid fungal biofilm developed in more than 75% of sinuses associated with bacterial biofilm of all species, except Haemophilus influenzae, which failed to establish bacterial biofilm. Fungal biofilm also established in association with cilia toxin. Significant cilial damage was incited by all bacterial biofilms and cilia toxin, and was associated with fungal proliferation. Fungal biofilm formation did not significantly increase mucosal inflammation or epithelial damage over that caused by the bacteria or cilia toxin alone. Conclusion Bacterial biofilms cause sinonasal mucosal inflammation and epithelial injury, which provides conditions appropriate for fungal biofilm proliferation. The role of cilia in sinonasal mucosal defense against fungal organisms has been demonstrated. Without such an insult, fungal biofilms fail to proliferate in occluded sinuses. Improving cilial recovery postoperatively and treating bacterial biofilms may be key factors in reducing recalcitrance in allergic fungal rhinosinusitis patients.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::f37b6bb64e29bb950c5643725b6c2a7aTest
https://doi.org/10.1002/alr.21138Test -
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المؤلفون: Edward John, Cleland, Amanda, Drilling, Ahmed, Bassiouni, Craig, James, Sarah, Vreugde, Peter-John, Wormald
المصدر: International forum of allergyrhinology. 4(4)
مصطلحات موضوعية: Mice, Inbred C57BL, Mice, Staphylococcus aureus, Hyperplasia, Microbiota, Probiotics, Chronic Disease, Staphylococcus epidermidis, Animals, Cell Count, Goblet Cells, Staphylococcal Infections, Rhinitis
الوصف: Staphylococcus aureus (SA) is a key pathogenic component of the chronic rhinosinusitis (CRS) microbiome and is associated with increased disease severity and poor postoperative outcomes. Probiotic treatments potentially offer a novel approach to the management of pathogenic bacteria in these recalcitrant patients through supporting a healthy community of commensal species. This study aims to investigate the probiotic properties of Staphylococcus epidermidis (SE) against SA in a mouse model of sinusitis.Twenty C57/BL6 mice received intranasal inoculations of phosphate buffered saline (PBS), SE, SA, or a combination of SE and SA (SE+SA) for 3 days. Following euthanasia, the mouse snouts were harvested and prepared for histological analysis. Counts of periodic acid-Schiff (PAS)-positive goblet cells were the primary outcome measure.Goblet cell counts were significantly higher in both the SA and SE+SA groups compared to those receiving PBS or SE alone (p0.05). However, the SE+SA group demonstrated significantly lower goblet cell counts compared to the SA group (p0.05). Mice receiving SE alone did not show a significant difference to those receiving PBS (p0.05). The presence of SA postinoculation was confirmed by culture in both the SA and SE+SA groups.This study confirms the probiotic potential of SE against SA in a mouse model of sinusitis. Although the interactions that occur between many probiotic species and pathogens are yet to be fully understood, studies such as this support further exploration of ecologically-based treatment paradigms for the management of CRS.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::cb94a36846929368afa36c3d436dc275Test
https://pubmed.ncbi.nlm.nih.gov/24415658Test -
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المؤلفون: Edward John, Cleland, Ahmed, Bassiouni, Ahmed, Bassioni, Samuel, Boase, Scot, Dowd, Sarah, Vreugde, Peter-John, Wormald
المصدر: International forum of allergyrhinology. 4(4)
مصطلحات موضوعية: Male, Microbiota, Chronic Disease, Fungi, Humans, Female, Biodiversity, Postoperative Period, Middle Aged, Sinusitis, DNA, Fungal, DNA, Ribosomal, Rhinitis
الوصف: Our understanding of fungi in chronic rhinosinusitis (CRS) has been limited by previously employed detection techniques. This study examines the fungal component of the microbiome in CRS patients and controls using a highly sensitive culture-independent molecular technique. The aims of this study include the characterization of fungal richness, prevalence, abundance, temporal changes, and their relationship with patient outcomes.Swabs were collected from the sinuses of 23 CRS patients and 11 controls. Collection occurred intraoperatively, and at 6 and 12 weeks postoperatively. DNA was extracted from the swabs and fungal outcomes were determined through 18S ribosomal DNA (rDNA) fungal tag-encoded FLX amplicon pyrosequencing.Fungi were ubiquitous to all patients. A total of 207 fungal genera were detected, with a mean sample richness of 8.18 and 12.14 in the control and CRS groups, respectively. Malassezia was detected in all patients at surgery and was also the most abundant. Postoperatively, fungal richness decreased (p0.05) and was associated with declines in the prevalence of Fusarium and Neocosmospora (p0.05). Neocosmospora was also less abundant postoperatively (p0.05). No correlations were found with quality of life.This is the first study to use a highly sensitive pyrosequencing technique to reveal the true diversity of fungi in the sinuses of CRS patients and postoperative changes in richness. The presence of Malassezia, a genus not previously described in the sinuses, is of great interest, and its potential as a disease modifier should see further investigation given its association with atopic disease.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::25a743ddd040445a707f56bdf221b67bTest
https://pubmed.ncbi.nlm.nih.gov/24500871Test -
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المؤلفون: Peter J. Wormald, Edward John Cleland, Neil C.-W. Tan, Brendan C. Hanna, Camille Jardaleza, Ahmed Bassiouni
المصدر: Otolaryngology–Head and Neck Surgery. 147
مصطلحات موضوعية: Via canine fossa, medicine.medical_specialty, Maxillary sinus, business.industry, Dentistry, Surgery, medicine.anatomical_structure, Otorhinolaryngology, Canine fossa, medicine, business, Cadaveric spasm, Sinus (anatomy)
الوصف: Objective: 1) To assess how much of the maxilary sinus could be accessed through a 5-mm endoscopic canine fossa trephination (eCFT) in a cadaveric model. 2) Review the clinical benefits as well as ...
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::6f4a37845b925db7c9f8dbf5edaf38b0Test
https://doi.org/10.1177/0194599812451426a368Test