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1دورية أكاديمية
المؤلفون: Wójcik-Fatla, Angelina, Mackiewicz, Barbara, Sawczyn-Domańska, Anna, Sroka, Jacek, Siwiec, Jan, Paściak, Mariola, Szponar, Bogumiła, Pawlik, Krzysztof, Dutkiewicz, Jacek
المساهمون: Ministerstwo Nauki i Szkolnictwa Wyższego
المصدر: International Archives of Occupational and Environmental Health ; volume 95, issue 6, page 1179-1193 ; ISSN 0340-0131 1432-1246
مصطلحات موضوعية: Public Health, Environmental and Occupational Health
الوصف: Occurrence Gram-negative bacteria occur commonly in the inner tissues of stored coniferous and deciduous timber, showing a marked variation in numbers. The greatest maximal numbers are found in the sapwood of coniferous timber. The common constituents of the Gram-negative biota are potentially pathogenic species of Enterobacteriaceae family of the genera Rahnella , Pantoea , Enterobacter, and Klebsiella . The air of wood-processing facilities is polluted with the wood-borne Gram-negative bacteria and produced by them endotoxin, as demonstrated worldwide by numerous studies. Effects There are three potential pathways of the pathogenic impact of wood-borne Gram-negative bacteria on exposed woodworkers: allergic, immunotoxic, and infectious. Allergic impact has been underestimated for a long time with relation to Gram-negative bacteria. Hopefully, the recent demonstration of the first documented case of hypersensitivity pneumonitis (HP) in woodworkers caused by Pantoea agglomerans which developed in extremely large quantities in birch sapwood, would speed up finding of new wood-related cases of HP caused by Gram-negative bacteria. The second pathway is associated with endotoxin, exerting strong immunotoxic (excessively immunostimulative) action. It has been demonstrated that endotoxin is released into wood dust in the form of nano-sized microvesicles, by peeling off the outer membrane of bacteria. Endotoxin microvesicles are easily inhaled by humans together with dust because of small dimensions and aerodynamic shape. Afterwards, they cause a nonspecific activation of lung macrophages, which release numerous inflammatory mediators causing an inflammatory lung reaction, chest tightness, fever, gas exchange disorders, and bronchospasm, without radiographic changes. The resulting disease is known as “Organic Dust Toxic Syndrome” or “toxic pneumonitis.” The potential third pathway of pathogenic impact is infection. The suspected species is Klebsiella pneumoniae that may occur commonly in wood dust; however, ...
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5دورية أكاديمية
المؤلفون: Wójcik-Fatla Angelina, Sroka Jacek, Zając Violetta, Zwoliński Jacek, Sawczyn-Domańska Anna, Kloc Anna, Bilska-Zając Ewa, Chmura Robert, Dutkiewicz Jacek
المصدر: Journal of Veterinary Research, Vol 62, Iss 4, Pp 477-483 (2018)
مصطلحات موضوعية: veterinarians, toxoplasma gondii, leptospira spp., coxiella burnetii, echinococcus granulosus, Veterinary medicine, SF600-1100
الوصف: Introduction: Exposure to zoonotic factors in veterinary practice is closely related to the nature of the work. The main aim of the study was to determine the risk of selected zoonotic infections among the occupational group of veterinarians in Poland.
وصف الملف: electronic resource
العلاقة: https://doaj.org/toc/2450-8608Test
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6دورية أكاديمية
المؤلفون: Lemieszek, Marta Kinga, Sawa-Wejksza, Katarzyna, Golec, Marcin, Dutkiewicz, Jacek, Zwoliński, Jacek, Milanowski, Janusz
المساهمون: Staab-Weijnitz, Claudia, Narodowe Centrum Nauki
المصدر: PLOS ONE ; volume 16, issue 5, page e0251237 ; ISSN 1932-6203
الوصف: Cathelicidin (CRAMP) is a defence peptide with a wide range of biological responses including antimicrobial, immunomodulatory and wound healing. Due to its original properties the usefulness of CRAMP in the treatment of pulmonary fibrosis was assessed in a murine model of hypersensitivity pneumonitis (HP). The studies were conducted on mouse strain C57BL/6J exposed to a saline extract of Pantoea agglomerans cells (HP inducer). Cathelicidin was administered in the form of an aerosol during and after HP development. Changes in the composition of immune cell populations (NK cells, macrophages, lymphocytes: Tc, Th, Treg, B), were monitored in lung tissue by flow cytometry. Extracellular matrix deposition (collagens, hydroxyproline), the concentration of cytokines involved in inflammatory and the fibrosis process (IFNγ, TNFα, TGFβ1, IL1β, IL4, IL5, IL10, IL12α, IL13) were examined in lung homogenates by the ELISA method. Alterations in lung tissue morphology were examined in mouse lung sections stained with haematoxylin and eosin as well as Masson trichrome dyes. The performed studies revealed that cathelicidin did not cause any negative changes in lung morphology/structure, immune cell composition or cytokines production. At the same time, CRAMP attenuated the immune reaction induced by mice chronic exposure to P . agglomerans and inhibited hydroxyproline and collagen deposition in the lung tissue of mice treated with bacteria extract. The beneficial effect of CRAMP on HP treatment was associated with restoring the balance in quantity of immune cells, cytokines production and synthesis of extracellular matrix components. The presented study suggests the usefulness of cathelicidin in preventing lung fibrosis; however, cathelicidin was not able to reverse pathological changes completely.
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7دورية أكاديمية
المؤلفون: Sroka, Jacek, Karamon, Jacek, Wójcik-Fatla, Angelina, Piotrowska, Weronika, Dutkiewicz, Jacek, Bilska-Zając, Ewa, Zając, Violetta, Kochanowski, Maciej, Dąbrowska, Joanna, Cencek, Tomasz
المصدر: Parasites & Vectors ; volume 13, issue 1 ; ISSN 1756-3305
مصطلحات موضوعية: Infectious Diseases, Parasitology
الوصف: Background Toxoplasma gondii infection may pose a severe medical problem especially in a congenital form and as an acquired infection in immunocompromised persons. Raw and undercooked meat of slaughtered animals is regarded as an important source of parasite infection; however, data concerning this issue in Poland are still insufficient. The aim of this study was to estimate the prevalence of T. gondii infection in pigs and cattle slaughtered for human consumption in Poland using serological and molecular methods. Methods Sera of 3111 pigs and 2411 cattle from 16 regions (voivodeships) of the country were examined for the presence of anti- T. gondii IgG using the direct agglutination test (DAT). Pepsin-digested samples of diaphragm and heart of seropositive animals were examined for the presence of T. gondii DNA (B1 gene) by nested PCR and real-time PCR, while non-digested samples were only examined by nested PCR. The B1 gene DNA samples were genotyped at 11 genetic markers using multilocus nested PCR-RFLP (Mn-PCR-RFLP) and sequencing. Results Seropositive DAT results were found in 11.9% of pigs and 13.0% of cattle. The highest seroprevalence was found in pigs from Podkarpackie (32.6%) and in cattle from Mazowieckie (44.6%). Data analysis showed that cattle > 5–10 years-old, as well as cattle and pigs from small farms, and pigs from farms with open production systems, had higher odds of testing seropositive ( P < 0.05). Among the examined tissue samples, positive PCR results were found in samples from 12.2% and 10.2% of seropositive pigs and cattle, respectively. Among the samples successfully genotyped by Mn-PCR-RFLP and sequenced, four samples were identified as T. gondii type II and one sample as type I. Conclusions The presence of T. gondii antibodies in a substantial proportion of examined pigs and cattle as well as the detection of parasite DNA in their tissues highlight a potential health risk to the consumers in Poland.
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8دورية أكاديمية
المؤلفون: Sroka, Jacek, Bilska-Zając, Ewa, Wójcik-Fatla, Angelina, Zając, Violetta, Dutkiewicz, Jacek, Karamon, Jacek, Piotrowska, Weronika, Cencek, Tomasz
المصدر: Foodborne Pathogens and Disease ; volume 16, issue 3, page 195-204 ; ISSN 1535-3141 1556-7125
مصطلحات موضوعية: Animal Science and Zoology, Applied Microbiology and Biotechnology, Food Science, Microbiology
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9كتاب
المؤلفون: Dutkiewicz, Jacek (1982-2015).
المساهمون: Ogdowski, Marcin (1976- )., Wydawnictwo Ender Sławomir Brudny. pbl
مصطلحات موضوعية: Polska. Wojsko Polskie (1944- ). 17 Wielkopolska Brygada Zmechanizowana im. gen. broni Józefa Dowbor-Muśnickiego -- relacje osobiste
Subject Person: Dutkiewicz, Jacek (1982-2015) -- dzienniki intymne.
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المؤلفون: Pomorska, Dorota, Larsson, Lennart, Skorska, Czeslawa, Sitkowska, Jolanta, Dutkiewicz, Jacek
المصدر: Annals of Agricultural and Environmental Medicine. 14(2):291-298
مصطلحات موضوعية: 3-hydroxy fatty acids, Limulus test, spectrometry, mass, gas chromatography, animal houses, air, endotoxin, organic dust, Medicin och hälsovetenskap, Medicinska och farmaceutiska grundvetenskaper, Mikrobiologi inom det medicinska området, Medical and Health Sciences, Basic Medicine, Microbiology in the medical area
الوصف: Air samples were collected on glass fibre filters in 22 animal houses and 3 hay storage barns and examined for the presence of bacterial endotoxin with the Limulus (LAL) test and the gas chromatography - tandem mass spectrometry (GC-MSMS) technique, based on detection of 3-hydroxy fatty acids (3-OH-FAs) as chemical markers of the endotoxin lipopolysaccharide. The median concentrations of airborne endotoxin determined with LAL test in poultry houses, sheep sheds, piggeries, cow barns, and horse stables were respectively 62.49 mu g/m(3), 26.2 mu g/m(3), 3.8 mu g/m(3), 1.65 mu g/m(3), and 1. 14 mu g/m(3), while those determined with the GC-MSMS technique were respectively 1.06 mu g/m(3), 7.91 mu g/m(3), 0.2 mu g/m(3), 0.31 mu g/m(3), and 1.42 mu g/m(3). The median concentrations of airborne endotoxin determined with LAL test and GC-MSMS technique in hay storage barns were much smaller, 0.09 mu g/m(3) and 0.03 mu g/m(3), respectively. The concentrations of airborne endotoxin (LPS) detected with GC-MSMS method in the air of sheep sheds were significantly greater than in all other examined facilities, while those detected in hay storage barns were significantly smaller than in all other examined facilities (p<0.05). The concentrations of airborne endotoxin determined with LAL test and GC-MSMS analysis exceeded in most of animal houses examined (91% by each method) the threshold limit value for airborne endotoxin of 0.1 mu g/m(3) proposed by various authors. A significant correlation (p<0.05) between the concentrations of endotoxin determined with the LAL and GC-MSMS techniques was found in the air samples collected in poultry houses and sheep sheds, but not in other examined facilities. 3-OH FAs with C-14-C-18 chains were predominant in the air of the facilities under study. A significant correlation (p<0.05) was found between the concentrations of endotoxin determined with LAL test and the amounts of 3-OH FAs with C-14-C-16 chains. In conclusion, endotoxin in the concentrations detected in this study may present a respiratory hazard to both humans and livestock animals.
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