المؤلفون: Lobato-Márquez, Damián, Conesa, José Javier, López-Jiménez, Ana Teresa, Divine, Michael E, Pruneda, Jonathan N, Mostowy, Serge

الوصف: During host cell invasion, Shigella escapes to the cytosol and polymerizes actin for cell-to-cell spread. To restrict cell-to-cell spread, host cells employ cell-autonomous immune responses including antibacterial autophagy and septin cage entrapment. How septins interact with the autophagy process to target Shigella for destruction is poorly understood. Here, we employed a correlative light and cryo-soft X-ray tomography (cryo-SXT) pipeline to study Shigella septin cage entrapment in its near-native state. Quantitative cryo-SXT showed that Shigella fragments mitochondria and enabled visualization of X-ray-dense structures (∼30 nm resolution) surrounding Shigella entrapped in septin cages. Using Airyscan confocal microscopy, we observed lysine 63 (K63)-linked ubiquitin chains decorating septin-cage-entrapped Shigella. Remarkably, septins and K63 chains are present in separate bacterial microdomains, indicating they are recruited separately during antibacterial autophagy. Cryo-SXT and live-cell imaging revealed an interaction between septins and LC3B-positive membranes during autophagy of Shigella. Together, these findings demonstrate how septin-caged Shigella are targeted for autophagy and provide fundamental insights into autophagy-cytoskeleton interactions.

وصف الملف: text

العلاقة: https://researchonline.lshtm.ac.uk/id/eprint/4669934/1/Marquez-etal-2023-Septins-and-k63-ubiquitin-chains.pdfTest; Lobato-Márquez, Damián; Conesa, José Javier; López-Jiménez, Ana Teresa; Divine, Michael E; Pruneda, Jonathan N; Mostowy, Serge ; (2023) Septins and K63 ubiquitin chains are present in separate bacterial microdomains during autophagy of entrapped Shigella. Journal of cell science, 136 (7). jcs261139-. ISSN 0021-9533 DOI: https://doi.org/10.1242/jcs.261139Test

الإتاحة: https://doi.org/10.1242/jcs.261139Test
https://researchonline.lshtm.ac.uk/id/eprint/4669934Test/
https://researchonline.lshtm.ac.uk/id/eprint/4669934/1/Marquez-etal-2023-Septins-and-k63-ubiquitin-chains.pdfTest

المؤلفون: Lobato-Márquez, Damián, Javier Conesa, José, López-Jiménez, Ana Teresa, Divine, Michael E., Pruneda, Jonathan N., Mostowy, Serge

المساهمون: HORIZON EUROPE European Research Council, National Institute of General Medical Sciences, Atracción de Talento, Wellcome Trust

المصدر: Autophagy Reports ; volume 2, issue 1 ; ISSN 2769-4127

الإتاحة: https://doi.org/10.1080/27694127.2023.2213541Test