المؤلفون: Dini, Germana, Schiavone, Nicola, Magnelli, Lucia

المصدر: The FASEB Journal ; volume 23, issue S1 ; ISSN 0892-6638 1530-6860

الوصف: The effect on drug sensitivity of FGF‐2 expression was evaluated in a murine fibroblast (Balb/A31) and in a human melanoma (MC) cell line. Human metastatic melanoma cells, negative for FGF‐2 expression, were transfected in order to express low (18 kDa), high molecular weight (HMW) and all (ALL) isoforms. Clonogenic assay showed that 18 kDa expression was related to chemoresistance to Dacarbazine (DITC), both in 18 kDa and ALL MC cells with respect to the mock transfected cell line. In contrast, the expression of High Molecular Weigth FGF‐2 isoforms alone produced a chemosensitive phenotype, even if compared to that of FGF‐2‐negative clones. Dacarbazine‐resistant clones were obtained following drug selection confirming that resistance was induced by 18 kDa low molecular weight isoform while high molecular weight isoforms produced a sensitive phenotype. Accordingly high molecular weight isoforms were lost and low molecular isoform was gained from DITC resistant ALL and 18 kDa cell line, respectively. Also we failed to obtain resistant clones from HMW cells. Balb/A31 exogenously expressing different human FGF‐2 isoforms were treated with increasing concentration of doxorubicin, cisplatin and 5‐fluorouracyl. Clonogenic survival was evaluated to determine drug resistance. Results showed a highly variable response dependent on both FGF‐2 isoform expression pattern and treatment. Grant: CRF 2003.0808

الإتاحة: https://doi.org/10.1096/fasebj.23.1_supplement.925.17Test

المؤلفون: Fibbi, Gabriella, Barletta, Emanuela, Dini, Germana, Del Rosso, Angela, Pucci, Marco, Cerletti, Massimiliano, Del Rosso, Mario

المصدر: Laboratory Investigation ; volume 81, issue 1, page 27-39 ; ISSN 0023-6837

مصطلحات موضوعية: Cell Biology, Molecular Biology, Pathology and Forensic Medicine

الإتاحة: https://doi.org/10.1038/labinvest.3780209Test
http://www.nature.com/articles/3780209.pdfTest
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المؤلفون: Del Rosso, Mario, Fibbi, Gabriella, Dini, Germana, Grappone, Cecilia, Pucci, Marco, Caldini, Riccardo, Magnelli, Lucia, Fimiani, Michele, Lotti, Torello, Panconesi, Emiliano

المصدر: Journal of Investigative Dermatology ; volume 94, issue 3, page 310-316 ; ISSN 0022-202X

مصطلحات موضوعية: Cell Biology, Dermatology, Molecular Biology, Biochemistry

الإتاحة: https://doi.org/10.1111/1523-1747.ep12874442Test
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المؤلفون: Dini, Germana, Funghini, Silvia, Witort, Ewa, Magnelli, Lucia, Fanti, Elena, Rifkin, Daniel B., Del Rosso, Mario

المصدر: Journal of Cellular Physiology ; volume 193, issue 1, page 64-72 ; ISSN 0021-9541 1097-4652

الوصف: We investigated the role of low molecular weight (LMW) and high molecular weight (HMW) isoforms of basic fibroblast growth factor 2 (FGF‐2) in the expression of transformation‐related phenotypic alterations, drug sensitivity modulation, and gene amplification potential. For this purpose, we used NIH 3T3 and A31 cells transfected with different cDNA FGF‐2 constructs allowing expression of the different proteins. Both cell lines showed marked phenotypic alterations when expressing the LMW FGF‐2 or the four HMW FGF‐2 isoforms: they acquired a transformed morphology, grew at higher saturation densities in 10% serum, and exhibited anchorage‐independent growth and increased invasive potential. However, HMW FGF‐2‐expressing cells also grew in 1% serum and their invasive potential was lower than in cells expressing all FGF‐2 forms or LMW FGF‐2 alone. We have grown the different cell lines under a selective pressure of N ‐(phosphonacetyl)‐ l ‐aspartate (PALA), a drug which specifically inhibits the aspartate transcarbamylase activity of the multifunctional carbamyl‐ P ‐synthetase/aspartate transcarbamylase/dihydro‐orotase genes (CAD) enzyme (and thus inhibits de novo pyrimidine biosynthesis) and selects for cells with amplified copies of the CAD gene. Our results demonstrate that aberrant expression of the LMW FGF‐2 and/or HMW FGF‐2 isoforms differently modulates drug resistance and gene amplification properties in the NIH 3T3 and A31 cell lines by differential amplification of the CAD gene. Coexpression of all isoforms appears to be necessary to obtain cumulative effects and nuclear‐targeted HMW FGF‐2 has a pivotal role in such a cooperation. J. Cell. Physiol. 193: 64–72, 2002. © 2002 Wiley‐Liss, Inc.

الإتاحة: https://doi.org/10.1002/jcp.10152Test

المؤلفون: Cerinic, Marco Matucci, Generini, Sergio, Partsch, Gerald, Pignone, Alberto, Dini, Germana, Konttinen, Yrjo T., Del Rosso, Mario

المصدر: Life Sciences ; volume 63, issue 6, page 441-453 ; ISSN 0024-3205

مصطلحات موضوعية: General Pharmacology, Toxicology and Pharmaceutics, General Biochemistry, Genetics and Molecular Biology, General Medicine

الإتاحة: https://doi.org/10.1016/s0024-3205Test(98)00293-8
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المؤلفون: Baccaro, Cecilia, Bennardini, F., Dini, Germana, Franconi, Flavia, Giotti, A., Matucci, Rosanna, Minuti, Paola

المصدر: British Journal of Pharmacology ; volume 87, issue 4, page 649-656 ; ISSN 0007-1188 1476-5381

الإتاحة: https://doi.org/10.1111/j.1476-5381.1986.tb14581.xTest

المؤلفون: GHERSETICH, ILARIA, LOTTI, TORELLO, CAMPANILE, GRAZIA, GRAPPONE, CECILIA, DINI, GERMANA

المصدر: International Journal of Dermatology ; volume 33, issue 2, page 119-122 ; ISSN 0011-9059 1365-4632

الوصف: Background. In elderly individuals all components of the skin and subcutaneous tissue undergo histologic and ultra‐structural changes. The turgidity of the dermis appears decreased, presumably due to altered patterns and levels of glycosaminoglycans (GAGS), especially hyaluronic acid and dermatan sulfate that are the most common. A linear, age‐related decrease in the content of GAGS (mainly hyaluronic acid) has been hypothesized in human aged skin. Methods. We used the cationic dye Alcian Blue to selectively stain hyaluronic acid within the dermis in old and young subjects to compare ultrastructurally its topography and variations with age. Results. We demonstrated a progressive reduction in the number of electron‐dense granules of hyaluronic acid and of their filaments until they were completely absent in subjects aged 60. Conclusions. We propose that the variations of the levels of hyaluronic acid in the dermis in aging could account for some of the most striking alterations of the aged skin, including decreased turgidity, less support for microvessles, wrinkling, and altered elasticity.

الإتاحة: https://doi.org/10.1111/j.1365-4362.1994.tb01540.xTest

المؤلفون: Petruzzi, Enrico, Orlando, Claudio, Pinzani, Pamela, Sestini, Roberta, Del Rosso, Attilio, Dini, Germana, Tanganelli, Elisabetta, Buggiani, Antonio, Pazzagli, Mario

المصدر: Metabolism ; volume 43, issue 4, page 435-440 ; ISSN 0026-0495

مصطلحات موضوعية: Endocrinology, Endocrinology, Diabetes and Metabolism

الإتاحة: https://doi.org/10.1016/0026-0495Test(94)90072-8
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المؤلفون: Lodovici, Maura, Dolara, Piero, Amerini, Sandra, Mantelli, Laura, Ledda, Fabrizio, Bennardini, Federico, Fazi, Marilena, Montereggi, Alessio, Dini, Germana

المصدر: European Journal of Pharmacology ; volume 243, issue 3, page 255-263 ; ISSN 0014-2999

مصطلحات موضوعية: Pharmacology

الإتاحة: https://doi.org/10.1016/0014-2999Test(93)90183-i
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المؤلفون: Rosso, Mario Del, Pedersen, Nina, Fibbi, Gabriella, Pucci, Marco, Dini, Germana, Anichini, Enrica, Blasi, Francesco

المصدر: Experimental Cell Research ; volume 203, issue 2, page 427-434 ; ISSN 0014-4827

مصطلحات موضوعية: Cell Biology

الإتاحة: https://doi.org/10.1016/0014-4827Test(92)90017-3
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