المؤلفون: Asefifeyzabadi, Narges, Nguyen, Tiffany, Li, Houpu, Zhu, Kan, Yang, Hsin‐ya, Baniya, Prabhat, Lopez, Andrea Medina, Gallegos, Anthony, Hsieh, Hao‐Chieh, Dechiraju, Harika, Hernandez, Cristian, Schorger, Kaelan, Recendez, Cynthia, Tebyani, Maryam, Selberg, John, Luo, Le, Muzzy, Elana, Hsieh, Cathleen, Barbee, Alexie, Orozco, Jonathan, Alhamo, Moyasar A, Levin, Michael, Aslankoohi, Elham, Gomez, Marcella, Zhao, Min, Teodorescu, Mircea, Isseroff, Roslyn Rivkah, Rolandi, Marco

مصطلحات موضوعية: Biomedical and Clinical Sciences, Clinical Sciences, Biotechnology, Bioengineering, bioelectronic device, ion pumps, wound healing, Dermatology & Venereal Diseases, Clinical sciences

الوصف: Wound healing is a complex physiological process that requires precise control and modulation of many parameters. Therapeutic ion and biomolecule delivery has the capability to regulate the wound healing process beneficially. However, achieving controlled delivery through a compact device with the ability to deliver multiple therapeutic species can be a challenge. Bioelectronic devices have emerged as a promising approach for therapeutic delivery. Here, we present a pro-reparative bioelectronic device designed to deliver ions and biomolecules for wound healing applications. The device incorporates ion pumps for the targeted delivery of H+ and zolmitriptan to the wound site. In vivo studies using a mouse model further validated the device's potential for modulating the wound environment via H+ delivery that decreased M1/M2 macrophage ratios. Overall, this bioelectronic ion pump demonstrates potential for accelerating wound healing via targeted and controlled delivery of therapeutic agents to wounds. Continued optimization and development of this device could not only lead to significant advancements in tissue repair and wound healing strategies but also reveal new physiological information about the dynamic wound environment.

الوصول الحر: https://escholarship.org/uc/item/7c59f5hbTest

المؤلفون: Shiu, Jessica, Lentsch, Griffin, Polleys, Christopher M, Mobasher, Pezhman, Ericson, Marissa, Georgakoudi, Irene, Ganesan, Anand K, Balu, Mihaela

المصدر: Journal of Investigative Dermatology. 144(4)

مصطلحات موضوعية: Biomedical and Clinical Sciences, Clinical Sciences, Humans, Vitiligo, Keratinocytes, Hypopigmentation, Melanocytes, Treatment Outcome, Keratinocyte, Melanocyte, Multiphoton microscopy, Reflectance confocal microscopy, Oncology and Carcinogenesis, Dermatology & Venereal Diseases, Clinical sciences

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/56t4f3zcTest

المؤلفون: Juhasz, Margit LW, Csuka, Ella, Kincaid, Colin, Mesinkovska, Natasha Atanaskova

المصدر: Dermatologic Surgery. 50(3)

مصطلحات موضوعية: Biomedical and Clinical Sciences, Clinical Sciences, Dentistry, Humans, Alopecia, Hair, Lasers, Dermatology & Venereal Diseases, Clinical sciences

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/0g70159rTest

المؤلفون: Minkis, Kira, Stratman, Erik J, Shi, Victoria J, Greywal, Tanya, Hu, Jenny C, Suozzi, Kathleen C, Saikaly, Sami K, Ortiz, Arisa, Kang, Bianca Y, Alam, Murad, members of the AACD Regulatory, Education, Kourosh, A Shadi, Boucher, Alison, Suggs, Amanda K, Bar, Anna, Cahn, Brian, Robinson, Carolyn A, Schlick, Cynthia A, Hooper, Deirdre, Antonovich, Diana, Bolotin, Diana, Hoss, Elika, Ghareeb, Erica, Hisham, Farhana Ikmal, Alvarez, Gabriella, Kuhn, Helena, Luke, Janiene, Roberts, Jared E, Joo, Jayne, Orringer, Jeffrey S, Labadie, Jessica G, Shah, Kalee, Ward, Kimberley HM, Kelly, Kristen M, Shahabi, Ladan, Kole, Lauren CS, Tolaymat, Leila, Dave, Loma, Council, M Laurin, Chow, Maggie L, Yi, Michael D, Pearlstein, Michelle V, Eshaq, Milad, Deng, Min, Lawrence, Naomi, Vashi, Neelam A, Kibbi, Nour, Schenck, Olivia L, Elsanadi, Rachel, Farah, Ronda S, Wyles, Saranya P, Yoo, Simon S, Nadir, Umer, Petronic-Rosic, Vesna, Levin, Yakir S, Nawas, Zeena Y

مصطلحات موضوعية: Biomedical and Clinical Sciences, Clinical Sciences, members of the AACD Regulatory, Resident Education, and Advocacy Working Group, Dermatology & Venereal Diseases, Clinical sciences

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/5fm4g6pvTest

المؤلفون: Dixon, Anthony J, Kyrgidis, Athanassios, Sladden, Michael, Nirenberg, Alexander, Steinman, Howard K, Smith, Harvey, Zachary, Christopher B, Anderson, Stuart, Leiter‐Stöppke, Ulrike, Longo, Caterina, Apalla, Zoe

مصطلحات موضوعية: Biomedical and Clinical Sciences, Clinical Sciences, Dermatology & Venereal Diseases, Clinical sciences

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/9wq3x3n1Test

المؤلفون: Soliman, John, Elsanadi, Rachel, Messele, Feben, Kelly, Kristen M

المصدر: Lasers in Medical Science. 39(1)

مصطلحات موضوعية: Engineering, Biomedical Engineering, Clinical Research, Pain Research, Clinical Trials and Supportive Activities, Chronic Pain, Humans, Low-Level Light Therapy, Prospective Studies, Wound Healing, Skin, Pain, Ablative fractional laser, LED treatment, Light-emitting diode, Wound healing, Dermatology & Venereal Diseases, Biomedical engineering

الوصف: Objective of the study is to assess the effects of wound healing with a commercially available light emitting diode (LED) photo biomodulation (PBM) device that emits three wavelengths (465, 640 and 880nm) after ablative fractional laser (AFL) treatment to healthy skin on the bilateral inner biceps. We conducted a prospective intraindividual randomized controlled study with 25 volunteers. AFL treatment was performed on healthy skin of the bilateral inner biceps. Subjects applied the LED light device for 30 min to the assigned bicep 3 times a week over 4 weeks, beginning on day 0. Subjects were followed up on days 2, 4, 6, 9, 13, 20 and 27 for treatment with the PBM device, clinical digital photography of the test and control sites, and in-person subject assessment, with follow ups on days 34 and 55 for clinical photography and assessment. Three blinded evaluators were asked to determine which bicep healed faster between day 0 to day 13. Pain, discomfort, and itch were also assessed. The three blinded evaluators chose the treatment arm as the faster healed arm in greater than 50% of the images, although the results were not statistically significant. There was no statistically significant difference between test and control arms in terms of pain, discomfort and itch. In conclusion, PBM therapy has the potential to improve wound healing. In this study, a three wavelength PBM device resulted in some subjects achieving faster healing after AFL but the results were not statistically significant.

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/8pp489z6Test

المؤلفون: King, Brett, Soung, Jennifer, Tziotzios, Christos, Rudnicka, Lidia, Joly, Pascal, Gooderham, Melinda, Sinclair, Rodney, Mesinkovska, Natasha A, Paul, Carle, Gong, Yankun, Anway, Susan D, Tran, Helen, Wolk, Robert, Zwillich, Samuel H, Lejeune, Alexandre

مصطلحات موضوعية: Clinical Sciences, Dermatology & Venereal Diseases, Clinical sciences

الوصف: BackgroundThe ALLEGRO phase 2a and 2b/3 studies demonstrated that ritlecitinib, an oral JAK3/TEC family kinase inhibitor, is efficacious at doses of ≥ 30 mg in patients aged ≥ 12 years with alopecia areata (AA).ObjectiveThe objective of this study was to evaluate the safety of ritlecitinib in an integrated analysis of four studies in AA.MethodsTwo cohorts were analyzed: a placebo-controlled and an all-exposure cohort. Proportions and study size-adjusted incidence rates (IRs) of adverse events (AEs) of interest and laboratory abnormalities are reported.ResultsIn the placebo-controlled cohort (n = 881; median exposure: 169 days), the proportion of ritlecitinib-treated patients with AEs was 70.2-75.4% across doses versus 69.5% in the placebo group; serious AEs occurred in 0-3.2% versus 1.9% for the placebo. A total of 19 patients permanently discontinued due to AEs (5 while receiving the placebo). In the all-exposure cohort (n = 1294), median ritlecitinib exposure was 624 days [2091.7 total patient-years (PY)]. AEs were reported in 1094 patients (84.5%) and serious AEs in 57 (4.4%); 78 (6.0%) permanently discontinued due to AEs. The most common AEs were headache (17.7%; 11.9/100 PY), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) positive test (15.5%; 9.8/100 PY), and nasopharyngitis (12.4%; 8.2/100 PY). There were two deaths (breast cancer and acute respiratory failure/cardiorespiratory arrest). Proportions (IRs) were < 0.1% (0.05/100 PY) for opportunistic infections, 1.5% (0.9/100 PY) for herpes zoster, 0.5% (0.3/100 PY) for malignancies (excluding nonmelanoma skin cancer), and 0.2% (0.1/100 PY) for major adverse cardiovascular events.ConclusionsRitlecitinib is well tolerated with an acceptable safety profile up to 24 months in patients aged ≥ 12 years with AA (video abstract and graphical plain language summary available).Trial registriesClinicalTrials.gov: NCT02974868 (date of registration: 11/29/2016), NCT04517864 (08/18/2020), NCT03732807 (11/07/2018), and NCT04006457 (07/05/2019).

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/03f3968vTest

المؤلفون: Shan, Shengzhou, He, Jiahao, Sun, Qin, Zhu, Kan, Li, Yuanyuan, Reid, Brian, Li, Qingfeng, Zhao, Min

المصدر: Experimental Dermatology. 32(12)

مصطلحات موضوعية: Biomedical and Clinical Sciences, Clinical Sciences, Bioengineering, Underpinning research, 1.1 Normal biological development and functioning, Skin, Optical Fibers, Epidermis, Cell Proliferation, Oxygen, Stress, Mechanical, cutaneous oxygen uptake, mechanical stretch, optical fiber sensor, skin expansion, surgery, Dermatology & Venereal Diseases, Clinical sciences

الوصف: Skin expands and regenerates in response to mechanical stretch. This important homeostasis process is critical for skin biology and can be exploited to generate extra skin for reconstructive surgery. Atmospheric oxygen uptake is important in skin homeostasis. However, whether and how cutaneous atmospheric oxygen uptake changes during mechanical stretch remains unclear, and relevant research tools to quantify oxygen flux are limited. Herein, we used the scanning micro-optrode technique (SMOT), a non-invasive self-referencing optical fiber microsensor, to achieve real-time measurement of cutaneous oxygen uptake from the atmosphere. An in vivo mechanical stretch-induced skin expansion model was established, and an in vitro Flexcell Tension system was used to stretch epidermal cells. We found that oxygen influx of skin increased dramatically after stretching for 1 to 3 days and decreased to the non-stretched level after 7 days. The enhanced oxygen influx of stretched skin was associated with increased epidermal basal cell proliferation and impaired epidermal barrier. In conclusion, mechanical stretch increases cutaneous oxygen uptake with spatial-temporal characteristics, correlating with cell proliferation and barrier changes, suggesting a fundamental mechanistic role of oxygen uptake in the skin in response to mechanical stretch. Optical fiber microsensor-based oxygen uptake detection provides a non-invasive approach to understand skin homeostasis.

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/367100w7Test

المؤلفون: Elsanadi, Rachel, Esse, Ilhan, Phong, Celine, Ortega, Alyssa Ashbaugh, Yale, Katerina, Mesinkovska, Natasha Atanaskova

المصدر: Journal of the American Academy of Dermatology. 89(6)

مصطلحات موضوعية: Biomedical and Clinical Sciences, Clinical Sciences, Humans, Alopecia Areata, Cross-Sectional Studies, Racial Groups, Ethnicity, Patient Selection, Clinical Trials as Topic, alopecia areata, clinical trial enroll-ment, clinical trial retention, race, racial dis-parities, skin of color, clinical trial enrollment, racial disparities, Dermatology & Venereal Diseases, Clinical sciences

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/22m1q4pdTest

المؤلفون: Widgerow, Alan D, Ziegler, Mary E, Garruto, John A, Shafiq, Faiza

مصطلحات موضوعية: Biomedical and Clinical Sciences, Clinical Sciences, Nutrition, Complementary and Integrative Health, Vit C salt, antioxidant, elastin-conserving, reactive oxygen species, Dermatology & Venereal Diseases, Clinical sciences

الوصف: BackgroundThis paper describes the background research and validation related to the formulation of a novel antioxidant product. Two defined outcomes were sought. Firstly, a combined efficacy of antioxidant ingredients in quenching free oxygen radicals. Secondly, the investigation into whether a vitamin C derivative sodium salt was elastin conserving in contrast to current vitamin C/l-ascorbic acid variations that have been reported to negatively affect elastin constitution and regeneration.Materials and methodsA leading l-ascorbic acid antioxidant available on the market was compared with the experimental new product in two studies. In the first experiment, the products were compared to assess their antioxidant properties. The evaluated products TOPICAL ANTIOXIDANT 1 and TOPICAL ANTIOXIDANT 2 were applied to human skin cultures (25-30 mg/cm2 ) for a total of 72 h of treatment and exposed to oxidative stress. The generation of free radicals was semi-quantitatively assessed by measuring the fluorescence intensity of the deacetylation and oxidation of the probe dichlorofluorescein diacetate (DCFH-DA). In the second experiment, an ex vivo skin model (derived from patients undergoing facelift procedures) was used to assess elastin preservation. Three skin explants were topically subjected to the two formulations daily for 7 days. The skin was then prepared and fixed for immunofluorescent assessment after staining with CD44 and tropoelastin antibodies. Images were then analyzed using ImageJ.ResultsA full description of the different components selected for the new formulation is presented. In the first study, the experimental formulation performed with absolute equivalence to the comparator in its radical quenching capacity; both showed extremely effective antioxidant function. In the second study, the comparator negatively affected the existing elastin with areas of breakdown and diminished staining. In contrast, the new formulation showed good conservation of healthy elastin in all sections demonstrating elastin preservation.ConclusionA new antioxidant formulation was carefully designed with multiple actives that show an equivalent antioxidant capacity to a leading product on the market. More importantly, the vitamin C component shows direct elastin conservation and improvement as opposed to the comparator, which had negative effects on elastin preservation. This is in keeping with little-known literature reports on vitamin C and its negative effects on elastin and validates the use of a sodium salt derivative, which appears to have protective effects on elastin. These findings support the overall regenerative extracellular matrix changes seen with TriHex® technology in other products.

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/4bf2j0hfTest