المؤلفون: Bajetto, Greta, Scutera, Sara, Menotti, Francesca, Banche, Giuliana, Chiaradia, Giuseppe, Turesso, Caterina, De Andrea, Marco, Vallino, Marta, van Es, Daan, Biolatti, Matteo, Dell’Oste, Valentina, Musso, Tiziana

المصدر: Polymers 16 (2024) 8 ; ISSN: 2073-4360

مصطلحات موضوعية: antiviral and antibacterial polymers, biomaterials, medical devices, plasticizers, polyvinyl chloride (PVC), vegetable oils

الوصف: The growing prevalence of bacterial and viral infections, highlighted by the recent COVID-19 pandemic, urgently calls for new antimicrobial strategies. To this end, we have synthesized and characterized a novel fatty acid epoxy-ester plasticizer for polymers, named GDE. GDE is not only sustainable and user-friendly but also demonstrates superior plasticizing properties, while its epoxy components improve the heat stability of PVC-based matrices. A key feature of GDE is its ability to confer antimicrobial properties to surfaces. Indeed, upon contact, this material can effectively kill enveloped viruses, such as herpes simplex virus type 1 (HSV-1) and the β-coronavirus prototype HCoV-OC43, but it is ineffective against nonenveloped viruses like human adenovirus (HAdV). Further analysis using transmission electron microscopy (TEM) on HSV-1 virions exposed to GDE showed significant structural damage, indicating that GDE can interfere with the viral envelope, potentially causing leakage. Moreover, GDE demonstrates antibacterial activity, albeit to a lesser extent, against notorious pathogens such as Staphylococcus aureus and Escherichia coli. Overall, this newly developed plasticizer shows significant potential as an antimicrobial agent suitable for use in both community and healthcare settings to curb the spread of infections caused by microorganisms contaminating physical surfaces.

وصف الملف: application/pdf

العلاقة: https://edepot.wur.nl/658129Test; https://research.wur.nl/en/publications/antimicrobial-efficacy-of-a-vegetable-oil-plasticizer-in-pvc-matrTest

الإتاحة: https://doi.org/10.3390/polym16081046Test
https://research.wur.nl/en/publications/antimicrobial-efficacy-of-a-vegetable-oil-plasticizer-in-pvc-matrTest

المؤلفون: Albano, Camilla, Biolatti, Matteo, Mazibrada, Jasenka, Pasquero, Selina, Gugliesi, Francesca, Lo Cigno, Irene, Calati, Federica, Bajetto, Greta, Riva, Giuseppe, Griffante, Gloria, Landolfo, Santo, Gariglio, Marisa, De Andrea, Marco, Dell’Oste, Valentina

المصدر: Frontiers in Cellular and Infection Microbiology ; volume 14 ; ISSN 2235-2988

مصطلحات موضوعية: Infectious Diseases, Microbiology (medical), Immunology, Microbiology

الوصف: Citrullination is an emerging post-translational modification catalyzed by peptidyl-arginine deiminases (PADs) that convert peptidyl-arginine into peptidyl-citrulline. In humans, the PAD family consists of five isozymes (PADs 1-4, 6) involved in multiple diseases, including cancer. Given that high-risk (hr) human papillomaviruses (HPVs) are the etiological agents of cervical cancer, in this study, we sought to determine whether PAD-mediated protein citrullination would play a functional role in the HPV-driven transformation of epithelial cells. Here we show that both total protein citrullination and PAD4 expression levels are significantly associated with cervical cancer progression. Specifically, epithelial immunostaining for PAD4 revealed an increasingly higher histoscore from low-grade (CIN1) to high-grade (CIN2, CIN3) cervical intraepithelial neoplasia, and invasive squamous cell carcinoma (SCC) lesions, raising the attractive possibility that PAD4 may be used as tumor staging markers. Furthermore, taking advantage of the epidermoid cervical cancer cell line CaSki, which harbors multiple copies of the integrated HPV16 genome, we show that the expression of E6 and E7 HPV oncoproteins is impaired by treatment with the pharmacological pan-PAD inhibitor BB-Cl-amidine. Consistently, p53 and p21, two targets of HPV oncoproteins, are upregulated by the PAD inhibitor, which undergoes cell growth arrest and apoptosis. Altogether, these findings highlight a novel mechanism by which hrHPVs alter host regulatory pathways involved in cell cycle and survival to gain viral fitness, raising the possibility that PADs may represent an attractive target for developing novel host-targeting antivirals effective in preventing cervical cancer progression.

الإتاحة: https://doi.org/10.3389/fcimb.2024.1359367Test

المؤلفون: Albano Camilla, Biolatti Matteo, Mazibrada Jasenka, Pasquero Selina, Gugliesi Francesca, Griffante Gloria, Lo Cigno Irene, Cat Genova Elena, Bajetto Greta, Landolfo Santo, Gariglio Marisa, De Andrea Marco, Dell'Oste Valentina

المساهمون: Albano Camilla, Biolatti Matteo, Mazibrada Jasenka, Pasquero Selina, Gugliesi Francesca, Griffante Gloria, Lo Cigno Irene, Cat Genova Elena, Bajetto Greta, Landolfo Santo, Gariglio Marisa, De Andrea Marco, Dell'Oste Valentina

العلاقة: ispartofbook:50° Congresso Nazionale della Società Italiana di Microbiologia - Programma scientifico; 50° Congresso Nazionale della Società Italiana di Microbiologia; firstpage:38; lastpage:38; numberofpages:1; https://hdl.handle.net/2318/1877058Test

الإتاحة: https://hdl.handle.net/2318/1877058Test

المؤلفون: Griffante Gloria, Hewelt-Belka Weronika, Albano Camilla, Gugliesi Francesca, Pasquero Selina, Castillo Pacheco Sergio F., Bajetto Greta, Trifiro' Linda, Porporato Paolo E., Mina Erica, Vallino Marta, Krapp Christian, Jakobsen Martin R., Purdy John, Von Einem Jens, Landolfo Santo, Dell'oste Valentina, Biolatti Matteo

المساهمون: Griffante Gloria, Hewelt-Belka Weronika, Albano Camilla, Gugliesi Francesca, Pasquero Selina, Castillo Pacheco Sergio F., Bajetto Greta, Trifiro' Linda, Porporato Paolo E., Mina Erica, Vallino Marta, Krapp Christian, Jakobsen Martin R., Purdy John, Von Einem Jens, Landolfo Santo, Dell'oste Valentina, Biolatti Matteo

العلاقة: ispartofbook:50° Congresso Nazionale della Società Italiana di Microbiologia - Abstract Book; 50° Congresso Nazionale della Società Italiana di Microbiologia; firstpage:45; lastpage:45; numberofpages:1; http://hdl.handle.net/2318/1876818Test

الإتاحة: http://hdl.handle.net/2318/1876818Test

المؤلفون: Pasquero, Selina, Gugliesi, Francesca, Biolatti, Matteo, Dell’Oste, Valentina, Albano, Camilla, Bajetto, Greta, Griffante, Gloria, Trifirò, Linda, Brugo, Bianca, Raviola, Stefano, Lacarbonara, Davide, Yang, Qiao, Sudeshna, Sen, Barasa, Leonard, Haniff, Hafeez, Thompson, Paul R., Landolfo, Santo, De Andrea, Marco

المساهمون: Neumann, Donna M, Università degli Studi di Torino, Ministero dell’Istruzione, dell’Università e della Ricerca, Fondazione Cassa di Risparmio di Vercelli

المصدر: PLOS Pathogens ; volume 19, issue 12, page e1011849 ; ISSN 1553-7374

الوصف: Herpes simplex virus 1 (HSV-1) is a neurotropic virus that remains latent in neuronal cell bodies but reactivates throughout an individual’s life, causing severe adverse reactions, such as herpes simplex encephalitis (HSE). Recently, it has also been implicated in the etiology of Alzheimer’s disease (AD). The absence of an effective vaccine and the emergence of numerous drug-resistant variants have called for the development of new antiviral agents that can tackle HSV-1 infection. Host-targeting antivirals (HTAs) have recently emerged as promising antiviral compounds that act on host-cell factors essential for viral replication. Here we show that a new class of HTAs targeting peptidylarginine deiminases (PADs), a family of calcium-dependent enzymes catalyzing protein citrullination, exhibits a marked inhibitory activity against HSV-1. Furthermore, we show that HSV-1 infection leads to enhanced protein citrullination through transcriptional activation of three PAD isoforms: PAD2, PAD3, and PAD4. Interestingly, PAD3-depletion by specific drugs or siRNAs dramatically inhibits HSV-1 replication. Finally, an analysis of the citrullinome reveals significant changes in the deimination levels of both cellular and viral proteins, with the interferon (IFN)-inducible proteins IFIT1 and IFIT2 being among the most heavily deiminated ones. As genetic depletion of IFIT1 and IFIT2 strongly enhances HSV-1 growth, we propose that viral-induced citrullination of IFIT1 and 2 is a highly efficient HSV-1 evasion mechanism from host antiviral resistance. Overall, our findings point to a crucial role of citrullination in subverting cellular responses to viral infection and demonstrate that PAD inhibitors efficiently suppress HSV-1 infection in vitro , which may provide the rationale for their repurposing as HSV-1 antiviral drugs.

الإتاحة: https://doi.org/10.1371/journal.ppat.1011849Test

المؤلفون: Fanunza, Elisa, Cheng, Adam Z., Auerbach, Ashley A., Stefanovska, Bojana, Moraes, Sofia N., Lokensgard, James R., Biolatti, Matteo, Dell'Oste, Valentina, Bierle, Craig J., Bresnahan, Wade A., Harris, Reuben S.

المساهمون: Goodrum, Felicia, HHS | NIH | National Institute of Allergy and Infectious Diseases, HHS | NIH | National Cancer Institute

المصدر: Journal of Virology ; volume 97, issue 8 ; ISSN 0022-538X 1098-5514

الوصف: The APOBEC3 family of DNA cytosine deaminases comprises an important arm of the innate antiviral defense system. The gamma-herpesviruses Epstein-Barr virus and Kaposi’s sarcoma-associated herpesvirus and the alpha-herpesviruses herpes simplex virus (HSV)-1 and HSV-2 have evolved an efficient mechanism to avoid APOBEC3 restriction by directly binding to APOBEC3B and facilitating its exclusion from the nuclear compartment. The only viral protein required for APOBEC3B relocalization is the large subunit of the ribonucleotide reductase (RNR). Here, we ask whether this APOBEC3B relocalization mechanism is conserved with the beta-herpesvirus human cytomegalovirus (HCMV). Although HCMV infection causes APOBEC3B relocalization from the nucleus to the cytoplasm in multiple cell types, the viral RNR (UL45) is not required. APOBEC3B relocalization occurs rapidly following infection suggesting the involvement of an immediate early or early (IE/E) viral protein. In support of this possibility, genetic (IE1 mutant) and pharmacologic (cycloheximide) strategies that prevent the expression of IE/E viral proteins also block APOBEC3B relocalization. In comparison, the treatment of infected cells with phosphonoacetic acid, which interferes with viral late protein expression, still permits A3B relocalization. These results combine to indicate that the beta-herpesvirus HCMV uses an RNR-independent, yet phenotypically similar, molecular mechanism to antagonize APOBEC3B. IMPORTANCE Human cytomegalovirus (HCMV) infections can range from asymptomatic to severe, particularly in neonates and immunocompromised patients. HCMV has evolved strategies to overcome host-encoded antiviral defenses to achieve lytic viral DNA replication and dissemination and, under some conditions, latency and long-term persistence. Here, we show that HCMV infection causes the antiviral factor, APOBEC3B, to relocalize from the nuclear compartment to the cytoplasm. This overall strategy resembles that used by related herpesviruses. However, the HCMV ...

الإتاحة: https://doi.org/10.1128/jvi.00781-23Test

المؤلفون: Pasquero, Selina, Gugliesi, Francesca, Griffante, Gloria, Dell’Oste, Valentina, Biolatti, Matteo, Albano, Camilla, Bajetto, Greta, Delbue, Serena, Signorini, Lucia, Dolci, Maria, Landolfo, Santo, De Andrea, Marco

المساهمون: Pasquero, Selina, Gugliesi, Francesca, Griffante, Gloria, Dell’Oste, Valentina, Biolatti, Matteo, Albano, Camilla, Bajetto, Greta, Delbue, Serena, Signorini, Lucia, Dolci, Maria, Landolfo, Santo, De Andrea, Marco

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/35288208; firstpage:105278; lastpage:105288; numberofpages:11; journal:ANTIVIRAL RESEARCH; http://hdl.handle.net/2318/1849302Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85126284506

الإتاحة: https://doi.org/10.1016/j.antiviral.2022.105278Test
http://hdl.handle.net/2318/1849302Test

المؤلفون: Spadavecchia Alessia, Leone Agata, Dell’Oste Valentina, Biolatti Matteo, Coscia Alessandra, Cresi Francesco, Peila Chiara, Rubino Carlotta, Bertino Enrico

المساهمون: Spadavecchia Alessia, Leone Agata, Dell’Oste Valentina, Biolatti Matteo, Coscia Alessandra, Cresi Francesco, Peila Chiara, Rubino Carlotta, Bertino Enrico

العلاقة: ispartofbook:17th International Workshop of Neonatology and Pediatrics; 17th International Workshop of Neonatology and Pediatrics; firstpage:1; lastpage:1; numberofpages:1; http://hdl.handle.net/2318/1837530Test

الإتاحة: http://hdl.handle.net/2318/1837530Test

المؤلفون: Spadavecchia Alessia, Leone Agata, Dell’Oste Valentina, Biolatti Matteo, Coscia Alessandra, Cresi Francesco, Peila Chiara, Rubino Carlotta, Bertino Enrico

المساهمون: Spadavecchia Alessia, Leone Agata, Dell’Oste Valentina, Biolatti Matteo, Coscia Alessandra, Cresi Francesco, Peila Chiara, Rubino Carlotta, Bertino Enrico

العلاقة: ispartofbook:CMV 2021-2nd Congress on Congenital CMV; CMV 2021-2nd Congress on Congenital CMV; firstpage:1; lastpage:1; numberofpages:1; http://hdl.handle.net/2318/1837538Test

الإتاحة: http://hdl.handle.net/2318/1837538Test

المؤلفون: Spadavecchia Alessia, Leone Agata, Dell’Oste Valentina, Biolatti Matteo, Coscia Alessandra, Cresi Francesco, Peila Chiara, Rubino Carlotta, Bertino Enrico

المساهمون: Spadavecchia Alessia, Leone Agata, Dell’Oste Valentina, Biolatti Matteo, Coscia Alessandra, Cresi Francesco, Peila Chiara, Rubino Carlotta, Bertino Enrico

العلاقة: ispartofbook:XVII Congresso Nazionale della Società italiana di Neonatologia; XVII Congresso Nazionale della Società italiana di Neonatologia; firstpage:1; lastpage:1; numberofpages:1; http://hdl.handle.net/2318/1837539Test

الإتاحة: http://hdl.handle.net/2318/1837539Test