المؤلفون: Chao Li, Lei Li, Yifan Li, Dehua Liu, Kaifei Han, Ranlyu Zhu, Yao Zhao, Qunshan Lu, Chunde Li

المصدر: Orthopaedic Surgery, Vol 16, Iss 7, Pp 1555-1561 (2024)

مصطلحات موضوعية: Osteoarthritis, Postoperative pain, Randomized controlled trial, Topical nonsteroidal anti‐inflammatory drug, Unicompartmental knee arthroplasty, Orthopedic surgery, RD701-811

الوصف: Objective How to minimize postoperative pain following knee replacement surgery has been a great challenge. This study was performed to evaluate the effect of applying a topical nonsteroidal anti‐inflammatory drug (NSAID) lateral to the incision for postoperative pain following unicompartmental knee arthroplasty (UKA). Methods The randomized controlled trial enrolled 100 patients from August 2023 to January 2024. One hundred patients who underwent UKA were randomized into two groups. The intervention group received a topical NSAID lateral to the incision postoperatively, and the control group received a placebo lateral to the incision postoperatively. The primary outcome measures were the amount of opioid consumption and the visual analogue scale (VAS) score (12, 24, 36, 48, and 72 h after operation) for pain. The secondary outcome measures were the American Knee Society Score (AKSS, preoperation and 1‐month follow‐up after operation), the time of first analgesic demand, side effects of opioids, operation time, postoperative stay, surgery‐related complications, and postoperative incision healing grade. Independent sample t test and paired sample t test were used to compare continuous data. Chi‐square test and Fisher's precision probability tests were used to analyze the categorical data. Results Ninety‐eight patients (intervention group, 48 patients; control group, 50 patients) were analyzed. Opioid consumption was significantly lower in the intervention group than in the control group during the first 12 h, 12 to 24 h, and 24 to 48 h postoperatively (p

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1757-7853Test; https://doaj.org/toc/1757-7861Test

الوصول الحر: https://doaj.org/article/fe30e1ae30ef4b20ac2b2a6a95768abcTest

المؤلفون: Ke Song, Liping Qi, Zongyou Mu, Houyi Sun, Shenhao Zhai, Dehua Liu, Shihao Li, Yange Luo, Peilai Liu

المصدر: Journal of Orthopaedic Surgery, Vol 32 (2024)

مصطلحات موضوعية: Orthopedic surgery, RD701-811

الوصف: Background While previous research has demonstrated potential advantages of unicompartmental knee arthroplasty (UKA) over total knee arthroplasty (TKA), particularly in terms of clinical outcomes such as function and pain relief, the specific impact on health-related quality of life (HRQOL) remains unclear. This systematic review and meta-analysis aim to address this gap by comparing HRQOL outcomes between UKA and TKA, providing valuable insights for clinical decision-making. Methods We conducted a literature search in the PubMed, Embase, Cochrane Controlled Register of Trials (CENTRAL), and Web of Science databases up to July 15, 2023. Eligible studies assessed HRQOL using EQ-5D, SF-36, or SF-12 and were assessed for methodological quality using the Newcastle-Ottawa Scale (NOS). Results Seven eligible studies were included, comprising a total of 64,585 patients with 35,809 undergoing TKA and 28,776 undergoing UKA. Patient age ranged from 52.0 to 67.7 years with an average BMI ranging from 27.2 to 31.0 kg/m 2 . Follow-up periods ranged from 6 months to 10 years. Five studies (63,829 patients) that evaluated HRQOL using EQ-5D showed significantly better outcomes for UKA compared to TKA (MD -0.04, 95% CI -0.05 to −0.02). Two studies (756 patients) that evaluated HRQOL using SF-36 showed no significant difference between TKA and UKA. Five studies (63,286 patients) that evaluated functional outcomes using Oxford Knee Score (OKS) showed significantly better functional scores for UKA compared to TKA (MD -1.29, 95% CI -1.86 to −0.72). Four studies (24,570 patients) that reported patient satisfaction showed no statistically significant difference between TKA and UKA (MD 0.97, 95% CI 0.90 to 1.05). Further subgroup analysis did not affect the conclusions. Conclusions Our meta-analysis suggests that UKA is associated with better HRQOL and knee function, as well as similar patient satisfaction, compared to TKA for patients with unicompartmental osteoarthritis.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2309-4990Test

الوصول الحر: https://doaj.org/article/49819f9389c14e5dbb880c2f0aacc4f4Test

المؤلفون: Rui Wang, Xiao Peng, Yixin Yuan, Baojie Shi, Yuan Liu, Hengxiao Ni, Wenzhi Guo, Qiwei Yang, Pingguo Liu, Jie Wang, Zhaojie Su, Shengnan Yu, Dehua Liu, Jinyan Zhang, Junjie Xia, Xueni Liu, Hao Li, Zhengfeng Yang, Zhihai Peng

المصدر: The Innovation, Vol 5, Iss 3, Pp 100599- (2024)

مصطلحات موضوعية: Science (General), Q1-390

الوصف: Elucidating the temporal process of immune remodeling under immunosuppressive treatment after liver transplantation (LT) is critical for precise clinical management strategies. Here, we performed a single-cell multi-omics analysis of peripheral blood mononuclear cells (PBMCs) collected from LT patients (with and without acute cellular rejection [ACR]) at 13 time points. Validation was performed in two independent cohorts with additional LT patients and healthy controls. Our study revealed a four-phase recovery process after LT and delineated changes in immune cell composition, expression programs, and interactions along this process. The intensity of the immune response differs between the ACR and non-ACR patients. Notably, the newly identified inflamed NK cells, CD14+RNASE2+ monocytes, and FOS-expressing monocytes emerged as predictive indicators of ACR. This study illuminates the longitudinal evolution of the immune cell landscape under tacrolimus-based immunosuppressive treatment during LT recovery, providing a four-phase framework that aids the clinical management of LT patients.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2666675824000377Test; https://doaj.org/toc/2666-6758Test

الوصول الحر: https://doaj.org/article/55c575f009f44537ba33cc793f350174Test

المؤلفون: Dehua Liu, Yongqin Tian, Yibo Xu, Wenyi Zhao, Xipeng Pan, Xu Ji, Mu Yang, Huihua Yang

المصدر: Cognitive Robotics, Vol 4, Iss , Pp 74-87 (2024)

مصطلحات موضوعية: Tire sidewall, Multi-scale, Diverse characters, YOLO, Character detection, Electronic computers. Computer science, QA75.5-76.95

الوصف: Driving safety is significant to building a people-oriented and harmonious society, Tires are one of the key components of a vehicle and the character information on the tire sidewall is critical to their storage and usage. However, due to the diverse and differentiated features of typographic fonts, simultaneously extracting comprehensive characteristics is an extremely challenging task. To effectively break through these performance degradation issues, a multi-scale tire sidewall text region detection algorithm based on YOLOv5 is introduced, called YOLOT, which fuses comprehensive feature information in both width and depth directions. In this study, we firstly propose the Width and Depth Awareness (WDA) module in the text region detection field and successfully integrated it with the FPN structure to form the WDA-FPN. The purpose of WDA-FPN is to empower the network to capture multi-scale and multi-shape features in images, thereby augmenting the algorithm’s abstraction and representation of image features and concurrently boosting its robustness and generalization performance. Experimental findings indicate that, compared to the primary algorithm, YOLOT achieves significant improvement in accuracy, providing a higher detection reliability. The dataset and code for the paper are available at: https://github.com/CloudeTest-dehua/YOLOT.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S266724132400003XTest; https://doaj.org/toc/2667-2413Test

الوصول الحر: https://doaj.org/article/ac11d1bcc1e043b98a1a6fc3ee64e550Test

المؤلفون: Ting Liu, Congjie Zhai, Bo Tian, Chao Li, Shuangshuang Han, Shihui Wang, Mingda Xuan, Dehua Liu, Yunxia Zhao, Hongyan Zhao, Weifang Yu, Jia Wang

المصدر: Scientific Reports, Vol 14, Iss 1, Pp 1-12 (2024)

مصطلحات موضوعية: Medicine, Science

الوصف: Abstract Roundabout guidance receptor 2 (Robo2) is closely related to malignant tumors such as pancreatic cancer and liver fibrosis, but there is no relevant research on the role of Robo2 in HCC. The study will further explore the function and mechanism of Robo2 and its downstream target genes in HCC. Firstly, Robo2 protein levels in human HCC tissues and paired adjacent normal liver tissues were detected. Then we established HepG2 and Huh7 hepatoma cell lines with knock-down Robo2 by transfection with lentiviral vectors, and examined the occurrence of EMT, proliferation and apoptosis abilities in HCC cells by western blot, flow cytometry, wound healing assay and TUNEL staining. Then we verified the interaction between Robo2 and its target gene by Co-IP and immunofluorescence co-staining, and further explored the mechanism of Robo2 and YB-1 by rescue study. The protein expression level of Robo2 in HCC was considerably higher than that in the normal liver tissues. After successfully constructing hepatoma cells with knock-down Robo2, it was confirmed that down-regulated Robo2 suppressed EMT and proliferation of hepatoma cells, and accelerated the cell apoptosis. High-throughput sequencing and validation experiments verified that YB-1 was the downstream target gene of Robo2, and over-expression of YB-1 could reverse the apoptosis induced by Robo2 down-regulation and its inhibitory effect on EMT and proliferation. Robo2 deficiency inhibits EMT and proliferation of hepatoma cells and augments the cell apoptosis by regulating YB-1, thus inhibits the occurrence of HCC and provides a new strategy for the treatment of HCC.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-2322Test

الوصول الحر: https://doaj.org/article/46c41747d2624a4db8c7ef1c380f90e9Test

المؤلفون: Tong Zheng, Dehua Liu, Ziyue Chu, Yange Luo, Qunshan Lu, Baoqing Zhang, Peilai Liu

المصدر: BMC Musculoskeletal Disorders, Vol 25, Iss 1, Pp 1-7 (2024)

مصطلحات موضوعية: Lateral unicompartmental knee arthroplasty, Valgus, Lower limb alignment, Patients’ outcome, Diseases of the musculoskeletal system, RC925-935

الوصف: Abstract Purpose The objective of this study was to investigate the correlation between lower limb alignment and patient outcomes after lateral unicompartmental knee arthroplasty (LUKA). Methods In this retrospective study, the information of 51 patients who underwent lateral UKA was collected after an average of 27months of follow-up (13 to 60 months). Evaluation indicators include the AKS and WOMAC score. The Kellgren-Lawrence grade is used to evaluate the severity of osteoarthritis, while the hip-knee-ankle (HKA) angle is utilized to measure the valgus angle of lower limb alignment. Result Patients with postoperative valgus (≥ 3°) alignment had the best outcomes, while those with varus (≤-3°) alignment had the worst outcomes (p

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2474Test

الوصول الحر: https://doaj.org/article/e8bac9d21e9d4aaf9903dcb7e9761ce8Test

المؤلفون: Xuebin Xu, Meng Lei, Dehua Liu, Muyu Wang, Longbin Lu

المصدر: IET Image Processing, Vol 17, Iss 14, Pp 4129-4141 (2023)

مصطلحات موضوعية: computer vision, convolutional neural nets, image segmentation, Photography, TR1-1050, Computer software, QA76.75-76.765

الوصف: Abstract Lung segmentation is an essential step in a computer‐aided diagnosis system for chest radiographs. The lung parenchyma is first segmented in pulmonary computer‐aided diagnosis systems to remove the interference of non‐lung regions while increasing the effectiveness of the subsequent work. Nevertheless, most medical image segmentation methods nowadays use U‐Net and its variants. These variant networks perform poorly in segmentation to detect smaller structures and cannot accurately segment boundary regions. A multi‐interaction feature fusion network model based on Kiu‐Net is presented in this paper to address this problem. Specifically, U‐Net and Ki‐Net are first utilized to extract high‐level and detailed features of chest images, respectively. Then, cross‐residual fusion modules are employed in the network encoding stage to obtain complementary features from these two networks. Second, the global information module is introduced to guarantee the segmented region's integrity. Finally, in the network decoding stage, the multi‐interaction module is presented, which allows to interact with multiple kinds of information, such as global contextual information, branching features, and fused features, to obtain more practical information. The performance of the proposed model was assessed on both the Montgomery County (MC) and Shenzhen datasets, demonstrating its superiority over existing methods according to the experimental results.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1751-9659Test; https://doaj.org/toc/1751-9667Test

الوصول الحر: https://doaj.org/article/d39816629e2e46f8b03332a8d5ea3692Test

المؤلفون: Dehua Liu, Tao Wang, Qingguo Wang, Peikang Dong, Xiaohong Liu, Qiang Li, Youkui Shi, Jingtian Li, Jin Zhou, Quan Zhang

المصدر: PeerJ, Vol 11, p e16222 (2023)

مصطلحات موضوعية: Sepsis-induced cardiomyopathy, TPT1, PPI, Hub gene, Medicine, Biology (General), QH301-705.5

الوصف: Introduction Sepsis is a life-threatening disease that damages multiple organs and induced by the host’s dysregulated response to infection with high morbidity and mortality. Heart remains one of the most vulnerable targets of sepsis-induced organ damage, and sepsis-induced cardiomyopathy (SIC) is an important factor that exacerbates the death of patients. However, the underlying genetic mechanism of SIC disease needs further research. Methods The transcriptomic dataset, GSE171564, was downloaded from NCBI for further analysis. Gene expression matrices for the sample group were obtained by quartile standardization and log2 logarithm conversion prior to analysis. The time series, protein-protein interaction (PPI) network, and functional enrichment analysis via Gene Ontology and KEGG Pathway Databases were used to identify key gene clusters and their potential interactions. Predicted miRNA-mRNA relationships from multiple databases facilitated the construction of a TF-miRNA-mRNA regulatory network. In vivo experiments, along with qPCR and western blot assays, provided experimental validation. Results The transcriptome data analysis between SIC and healthy samples revealed 221 down-regulated, and 342 up-regulated expressed genes across two distinct clusters. Among these, Tpt1, Mmp9 and Fth1 were of particular significance. Functional analysis revealed their role in several biological processes and pathways, subsequently, in vivo experiments confirmed their overexpression in SIC samples. Notably, we found TPT1 play a pivotal role in the progression of SIC, and silencing TPT1 showed a protective effect against LPS-induced SIC. Conclusion In our study, we demonstrated that Tpt1, Mmp9 and Fth1 have great potential to be biomarker of SIC. These findings will facilitated to understand the occurrence and development mechanism of SIC.

وصف الملف: electronic resource

العلاقة: https://peerj.com/articles/16222.pdfTest; https://peerj.com/articles/16222Test/; https://doaj.org/toc/2167-8359Test

الوصول الحر: https://doaj.org/article/199d37f9854f40eb971baa568f55991eTest

المؤلفون: Min Zhang, Mei Zhao, Pei Qiao, Dehua Liu, Qingrong Bai, Wei Guan, Yuwen Yang, Tingchang Zhao

المصدر: Microorganisms, Vol 12, Iss 4, p 682 (2024)

مصطلحات موضوعية: Acidovorax citrulli, bacterial fruit blotch, copper tolerance, copA, Biology (General), QH301-705.5

الوصف: Acidovorax citrulli populations exhibit genetic and phenotypic variations, particularly in terms of copper tolerance. Group I strains of A. citrulli generally exhibit higher copper tolerance compared to group II strains. This study aims to identify genes involved in copper tolerance to better understand the differences in copper tolerance between group I and group II strains. Representative strains pslb65 (group I) and pslbtw14 (group II) were selected for comparison. Deletion mutants of putative copper-tolerance genes and their corresponding complementary strains were constructed. The copper tolerance of each strain was evaluated using the minimum inhibitory concentration method. The results showed that the copA, copZ, cueR, and cueO genes played major roles in copper tolerance in A. citrulli, while cusC-like, cusA-like, and cusB-like genes had minor effects. The different expression levels of copper-tolerance-related genes in pslb65 and pslbtw14 under copper stress indicated that they had different mechanisms for coping with copper stress. Overall, this study provides insights into the mechanisms of copper tolerance in A. citrulli and highlights the importance of specific genes in copper tolerance.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2076-2607/12/4/682Test; https://doaj.org/toc/2076-2607Test

الوصول الحر: https://doaj.org/article/9169f19e544f4143a42d8a9c286b2420Test

المؤلفون: Yang Gao, Dehua Liu, Sichen Li, Liang Cheng, Jing Sun

المصدر: Applied Sciences, Vol 14, Iss 6, p 2411 (2024)

مصطلحات موضوعية: shale oil, CO2, huff and puff, on-line NMR, parameter optimization, Technology, Engineering (General). Civil engineering (General), TA1-2040, Biology (General), QH301-705.5, Physics, QC1-999, Chemistry, QD1-999

الوصف: The pore mobilization characteristics of CO2 when in shale reservoirs is an important indicator for evaluating the effectiveness of its application for enhanced recovery in shale reservoirs, and it is important to develop a comprehensive set of physical simulation methods that are consistent with actual field operations. This has underscored the need for efficient development techniques in the energy industry. The huff-n-puff seepage oil recovery method is crucial for developing tight oil reservoirs, including shale oil. However, the small pore size and low permeability of shale render conventional indoor experiments unsuitable for shale oil cores. Consequently, there is a need to establish a fully enclosed experimental method with a high detection accuracy to optimize the huff and puff process parameters. The NMR technique identifies oil and gas transport features in nanogaps, and in this study, we use low-field nuclear magnetic resonance (NMR) online displacement technology to conduct CO2 huff and puff experiments on shale oil, covering the gas injection, well stewing, and production stages. After conducting four rounds of huff-n-puff experiments, key process parameters were optimized, including the simmering time, huff-n-puff timing, number of huff-n-puff rounds, and the amount of percolant injected. The findings reveal that as the number of huff-n-puff rounds increases, the time required for well stabilization decreases correspondingly. However, the enhancement in recovery from additional huff-n-puff rounds becomes negligible after three rounds, showing only a 1.16% improvement. CO2 re-injection is required when the pressure falls to 70% of the initiaformation pressure to ensure efficient shale oil well development. This study also indicates that the most economically beneficial results are achieved when the injection volume of the huff-n-puff process is 0.44 pore volumes (PVs).

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2076-3417/14/6/2411Test; https://doaj.org/toc/2076-3417Test

الوصول الحر: https://doaj.org/article/8faa917d4544432aa177387b54de458aTest