المؤلفون: Jones, MB, Highlander, SK, Anderson, EL, Li, W, Dayrit, M, Klitgord, N, Fabani, MM, Seguritan, V, Green, J, Pride, DT, Yooseph, S, Biggs, W, Nelson, KE, Craig Venter, J

المصدر: Proceedings of the National Academy of Sciences of the United States of America. 112(45)

مصطلحات موضوعية: MD Multidisciplinary

الوصف: Observations from human microbiome studies are often conflicting or inconclusive. Many factors likely contribute to these issues including small cohort sizes, sample collection, and handling and processing differences. The field of microbiome research is moving from 16S rDNA gene sequencing to a more comprehensive genomic and functional representation through whole-genome sequencing (WGS) of complete communities. Here we performed quantitative and qualitative analyses comparingWGS metagenomic data from human stool specimens using the Illumina Nextera XT and Illumina TruSeq DNA PCR-free kits, and the KAPA Biosystems Hyper Prep PCR and PCR-free systems. Significant differences in taxonomy are observed among the four different next-generation sequencing library preparations using a DNA mock community and a cell control of known concentration. We also revealed biases in error profiles, duplication rates, and loss of reads representing organisms that have a high %G+C content that can significantly impact results. As with all methods, the use of benchmarking controls has revealed critical differences among methods that impact sequencing results and later would impact study interpretation. We recommend that the community adopt PCR-free-based approaches to reduce PCR bias that affects calculations of abundance and to improve assemblies for accurate taxonomic assignment. Furthermore, the inclusion of a known-input cell spike-in control provides accurate quantitation of organisms in clinical samples.

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/8j8874m1Test

المؤلفون: Miranda, M. E., Ksiazek, T. G., Retuya, T. J., Khan, Ali S., Sanchez, Anthony, Fulhorst, Charles F., Rollin, Pierre E., Calaor, A. B., Manalo, D. L., Roces, M. C., Dayrit, M. M., Peters, C. J.

المصدر: The Journal of Infectious Diseases, 1999 Feb 01. 179, S115-S119.

الوصول الحر: https://www.jstor.org/stable/30117612Test

المؤلفون: Samb, B., Evans, T., Dybul, M., Atun, R., Moatti, Jp, Nishtar, S., Wright, A., Celletti, F., Hsu, J., Kim, Jy, Brugha, R., Russell, A., Etienne, C., De, S, Mwase, T., Wang, Wj, Wright, J., Dare, L., Delfraissy, Jf, Boillot, F., Miege, P., Zhang, Xl, Rhatigan, J., Weintraub, R., Pun, S., Abe, C., Caceres, C., Camara, M., Coriat, B., D Almeida, C., Aleshkina, J., Murzalieva, G., Kadzandira, J., Hammami, N., Mwapasa, V., Chkhatarashvili, K., Buch, E., Miti, K., Kamenga, C., Elouma, Mse, Schwalbe, N., Greenberg, A., Frehywot, S., Markus, A., Goeman, L., Khan, A., Amati, J., Mwaura-Muiru, E., Ivers, Lc, Ellner, A., Shakow, A., Kley, Nc, Irwin, A., Sullivan, E., Baker, B., Cohn, J., Davis, P., Headley, J., Siplon, P., Dickinson, C., Pearson, M., Waddington, C., Boyer, S., Eboko, F., Orsi, F., Larouze, B., Le Loup, G., Ndubani, P., Simbaya, J., Boelaert, M., Cavalli, A., Ooms, G., Pirard, M., Polman, K., Damme, W., Dormael, M., Vermeiren, P., Teokul, W., Dlodlo, R., Fujiwara, P., Ruppol, S., Vella, S., Semigina, T., Corbett, E., Godfrey-Faussett, P., Spicer, N., Walt, G., Kanchanachitra, C., Philips, M., Riva, G., Kabuayi, Jp, Kiputsu, Ak, Ndongosieme, A., Koulla-Shiro, S., Samake, S., Bamba, Si, Coulibaly, Y., Traore, Mn, Berthe, Ibi, Wibulpolprasert, S., Chunharas, S., Jerome, G., Mukherjee, J., Nguini, Meo, Rao, Kd, Reddy, S., Nyirenda, L., Biesma, R., Bruen, C., Dicker, P., Walsh, A., Parsons, A., Mathole, T., Sanders, D., Raghavan, Ss, Mwanza, F., Michael, Eb, Richard, W., Banati, P., Blakley, M., Ingenkamp, N., Lansang, Ma, Low-Beer, D., Shakarishvili, G., Kayongo, A., Buse, K., Martineau, T., Dehne, K., Pett, I., Salama, P., Chilundo, B., Ndumbe, P., Atashili, J., Sow, Ps, Peter Hill, Brenzel, L., Babaley, M., Dayrit, M., Zoysa, I., Dyrhauge, J., Galichet, B., Kadama, P., Porignon, D., Russell, S., Tata, H., Lerberghe, W.

المصدر: ResearcherID

الوصف: Since 2000, the emergence of several large disease-specific global health initiatives (GHIs) has changed the way in which international donors provide assistance for public health. Some critics have claimed that these initiatives burden health systems that are already fragile in countries with few resources, whereas others have asserted that weak health systems prevent progress in meeting disease-specific targets. So far, most of the evidence for this debate has been provided by speculation and anecdotes. We use a review and analysis of existing data, and 15 new studies that were submitted to WHO for the purpose of writing this Report to describe the complex nature of the interplay between country health systems and GHIs. We suggest that this Report provides the most detailed compilation of published and emerging evidence so far, and provides a basis for identification of the ways in which GHIs and health systems can interact to mutually reinforce their effects. On the basis of the findings, we make some general recommendations and identify a series of action points for international partners, governments, and other stakeholders that will help ensure that investments in GHIs and country health systems can fulfil their potential to produce comprehensive and lasting results in disease-specific work, and advance the general public health agenda. The target date for achievement of the health-related Millennium Development Goals is drawing close, and the economic downturn threatens to undermine the improvements in health outcomes that have been achieved in the past few years. If adjustments to the interactions between GHIs and country health systems will improve efficiency, equity value for money, and outcomes in global public health, then these opportunities should not be missed.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::b7693f7aaceb22a6394cb6d642b9f9ffTest
http://www.documentation.ird.fr/hor/fdi:010046188Test

المؤلفون: Dayrit, M. M.

مصطلحات موضوعية: Research Article

جغرافية الموضوع: Geneva

الوصف: 80 ; 108

العلاقة: Bulletin of the World Health Organization; Suppl 2; 76; https://apps.who.int/iris/handle/10665/260618Test; PMC2305668

الإتاحة: https://apps.who.int/iris/handle/10665/260618Test

المؤلفون: Trabajo, Marianne E., Viola, G., Magboo, F., Roces, M.C., Dayrit, M.

المصدر: Journal of Clinical Epidemiology ; volume 50, page S33 ; ISSN 0895-4356

مصطلحات موضوعية: Epidemiology

الإتاحة: https://doi.org/10.1016/s0895-4356Test(97)87276-9
https://api.elsevier.com/content/article/PII:S0895435697872769?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0895435697872769?httpAccept=text/plainTest

المؤلفون: Zimicki, S., Hornik, R. C., Verzosa, C. C., Hernandez, J. R., de Guzman, E., Dayrit, M., Fausto, A., Lee, M. B., Abad, M.

مصطلحات موضوعية: Research Article

جغرافية الموضوع: Geneva

الوصف: 409 ; 422

العلاقة: Bulletin of the World Health Organization; 72; https://apps.who.int/iris/handle/10665/264050Test; PMC2486710; 8062399

الإتاحة: https://apps.who.int/iris/handle/10665/264050Test

المؤلفون: Lim-Quizon, M. C., Benabaye, R. M., White, F. M., Dayrit, M. M., White, M. E.

مصطلحات موضوعية: Research Article

جغرافية الموضوع: Geneva

الوصف: 745 ; 749

العلاقة: Bulletin of the World Health Organization; 72; https://apps.who.int/iris/handle/10665/263934Test; PMC2486571; 7955024

الإتاحة: https://apps.who.int/iris/handle/10665/263934Test

المؤلفون: Roces, M. C., White, M. E., Dayrit, M. M., Durkin, M. E.

مصطلحات موضوعية: Research Article

جغرافية الموضوع: Geneva

الوصف: 509 ; 514

العلاقة: Bulletin of the World Health Organization; 70; https://apps.who.int/iris/handle/10665/261562Test; PMC2393385; 1394785

الإتاحة: https://apps.who.int/iris/handle/10665/261562Test

المؤلفون: Dayrit, M. M.¹

المصدر: MMWR: Morbidity & Mortality Weekly Report. 12/30/1999, Vol. 48, p105-111. 7p.

مصطلحات موضوعية: *CONFERENCES & conventions, *DISEASES, *HEALTH promotion, *POISONS, *MALNUTRITION

مصطلحات جغرافية: ATLANTA (Ga.), GEORGIA

مستخلص: The article presents a report prepared by the Workgroup on Noninfectious Diseases for the "Conference on Global Disease Elimination and Eradication as Public Health Strategies," held in Atlanta, Georgia. It says that the implementation of programmes for the diseases are being affected by the degree of interdependence among communities in terms of community interventions and the resources from the health system budget. The workgroup noted the benefits of addressing toxic exposures, protein-energy malnutrition and micronutrient malnutrition.

المؤلفون: Dayrit M (AUTHOR), Taylor A (AUTHOR), Yan J (AUTHOR), Braichet J (AUTHOR), Zurn P (AUTHOR), Shainblum E (AUTHOR)

المصدر: Bulletin of the World Health Organization. Oct2008, Vol. 86 Issue 10, p739-739. 1p.

URL: 34727373