المؤلفون: Dudley, A, Sater, M, Le, P U, Trinh, G, Sadr, M S, Bergeron, J, Deleavey, G F, Bedell, B, Damha, M J, Petrecca, K

المصدر: Oncogene ; volume 33, issue 41, page 4952-4960 ; ISSN 0950-9232 1476-5594

مصطلحات موضوعية: Cancer Research, Genetics, Molecular Biology

الإتاحة: https://doi.org/10.1038/onc.2013.436Test
https://www.nature.com/articles/onc2013436.pdfTest
https://www.nature.com/articles/onc2013436Test

المؤلفون: Lackey, J. G., Ron, D., Damha, M. J., Harding, H. P.

المصدر: Nucleic Acids Symposium Series ; volume 52, issue 1, page 475-476 ; ISSN 0261-3166 1746-8272

مصطلحات موضوعية: General Medicine

الإتاحة: https://doi.org/10.1093/nass/nrn241Test
http://academic.oup.com/nass/article-pdf/52/1/475/3867290/nrn241.pdfTest

المؤلفون: Lackey, J. G., Damha, M. J.

المصدر: Nucleic Acids Symposium Series ; volume 52, issue 1, page 35-36 ; ISSN 0261-3166 1746-8272

مصطلحات موضوعية: General Medicine

الإتاحة: https://doi.org/10.1093/nass/nrn018Test
http://academic.oup.com/nass/article-pdf/52/1/35/3862663/nrn018.pdfTest

المؤلفون: Wahba, A. S., Damha, M. J., Hudson, R. H.E.

المصدر: Nucleic Acids Symposium Series ; volume 52, issue 1, page 399-400 ; ISSN 0261-3166 1746-8272

مصطلحات موضوعية: General Medicine

الإتاحة: https://doi.org/10.1093/nass/nrn203Test
http://academic.oup.com/nass/article-pdf/52/1/399/3866190/nrn203.pdfTest

المؤلفون: Wahba, A. S., Damha, M. J., Hudson, R. H.E.

المصدر: Nucleic Acids Symposium Series ; volume 52, issue 1, page 397-398 ; ISSN 0261-3166 1746-8272

مصطلحات موضوعية: General Medicine

الإتاحة: https://doi.org/10.1093/nass/nrn202Test
http://academic.oup.com/nass/article-pdf/52/1/397/3866184/nrn202.pdfTest

المؤلفون: Kalota, A., Karabon, L., Swider, C. R., Viazovkina, E., Elzagheid, M., Damha, M. J., Gewirtz, A. M.

مصطلحات موضوعية: Articles

الوصف: To be effective in vivo , antisense oligonucleotides (AS ON) should be nuclease resistant, form stable ON/RNA duplexes and support ribonuclease H mediated heteroduplex cleavage, all with negligible non-specific effects on cell function. We report herein that AS ONs containing a 2′-deoxy-2′-fluoro-β-d-arabinonucleic acid (2′F-ANA) sugar modification not only meet these criteria, but have the added advantage of maintaining high intracellular concentrations for prolonged periods of time which appears to promote longer term gene silencing. To demonstrate this, we targeted the c-MYB protooncogene's mRNA in human leukemia cells with fully phosphorothioated 2′F-ANA–DNA chimeras (PS-2′FANA–DNA) and compared their gene silencing efficiency with AS ON containing unmodified nucleosides (PS-DNA). When delivered by nucleofection, chemically modified ON of both types effected a >90% knockdown of c-MYB mRNA and protein expression, but the PS-2′F-ANA–DNA were able to accomplish this at 20% of the dose of the PS-DNA, and in contrast to the PS-AS DNA, their silencing effect was still present after 4 days after a single administration. Therefore, our data demonstrate that PS-2′F-ANA–DNA chimeras are efficient gene silencing molecules, and suggest that they could have significant therapeutic potential.

وصف الملف: text/html

العلاقة: http://nar.oxfordjournals.org/cgi/content/short/34/2/451Test; http://dx.doi.org/10.1093/nar/gkj455Test

الإتاحة: https://doi.org/10.1093/nar/gkj455Test
http://nar.oxfordjournals.org/cgi/content/short/34/2/451Test

المؤلفون: Garavis, M., El Khoury, R., Damha, M. J., Gonzalez, C.

الإتاحة: https://doi.org/10.13018/bmr34735Test
https://bmrb.io/data_library/summary/?bmrbId=34735Test

المؤلفون: Damha, M. J., Wilds, C. J., Noronha, A., Brukner, I., Borkow, G., Arion, D., Parniak, M. A.

المصدر: Journal of the American Chemical Society ; volume 120, issue 51, page 13545-13545 ; ISSN 0002-7863 1520-5126

مصطلحات موضوعية: Colloid and Surface Chemistry, Biochemistry, General Chemistry, Catalysis

الإتاحة: https://doi.org/10.1021/ja985535vTest

المؤلفون: Macdonald, P. M., Damha, M. J., Ganeshan, K., Braich, R., Zabarylo, S. V.

مصطلحات موضوعية: Articles

الوصف: 31P cross polarization (CP) magic angle spinning (MAS) nuclear magnetic resonance (NMR) spectra were acquired for various linear and branched di- and tri-nucleotides attached to a controlled pore glass (CPG) solid support. The technique readily distinguishes the oxidation state of the phosphorus atom (phosphate versus phosphite), the presence or absence of a protecting group attached directly to phosphorus (cyanoethyl), and other large changes in the phosphorus chemistry (phosphate versus phosphorothioate). However, differences in configurational details remote from the phosphorus atom, such as the attachment position of the ribose sugar (2′5′ versus 3′5′), or the particulars of the nucleotide bases (adenine versus uridine versus thymine), could not be resolved. When different stages of the oligonucleotide synthetic cycle were examined, 31P CPMAS NMR revealed that the cyanoethyl protecting group is removed during the course of chain assembly.

وصف الملف: text/html

العلاقة: http://nar.oxfordjournals.org/cgi/content/short/24/15/2868Test; http://dx.doi.org/10.1093/nar/24.15.2868Test

الإتاحة: https://doi.org/10.1093/nar/24.15.2868Test
http://nar.oxfordjournals.org/cgi/content/short/24/15/2868Test

المؤلفون: Hudson, R. H. E., Ganeshan, K., Damha, M. J.

المصدر: ACS Symposium Series ; Carbohydrate Modifications in Antisense Research ; page 133-152 ; ISSN 0097-6156 1947-5918 ; ISBN 9780841230569 9780841215047

الإتاحة: https://doi.org/10.1021/bk-1994-0580.ch009Test