المؤلفون: De Bock, Marijke, Leybaert, Luc, Giaume, Christian

المصدر: NEUROCHEMICAL RESEARCH ; ISSN: 0364-3190 ; ISSN: 1573-6903

مصطلحات موضوعية: Biology and Life Sciences, Connexin, Gliovascular interface, Astrocyte, Blood-brain barrier, Inflammation, MICROVASCULAR ENDOTHELIAL-CELLS, FIBROBLAST-GROWTH-FACTOR, GAP-JUNCTION CHANNELS, NECROSIS-FACTOR-ALPHA, EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS, DEPENDENT GLUTAMATE RELEASE, INTERCELLULAR CA2+ WAVES, LOW EXTRACELLULAR CA2+, IN-VITRO MODELS, BARRIER INTEGRITY

الوصف: Neuronal survival, electrical signaling and synaptic activity require a well-balanced micro-environment in the central nervous system. This is achieved by the blood-brain barrier (BBB), an endothelial barrier situated in the brain capillaries, that controls near-to-all passage in and out of the brain. The endothelial barrier function is highly dependent on signaling interactions with surrounding glial, neuronal and vascular cells, together forming the neuro-glio-vascular unit. Within this functional unit, connexin (Cx) channels are of utmost importance for intercellular communication between the different cellular compartments. Connexins are best known as the building blocks of gap junction (GJ) channels that enable direct cell-cell transfer of metabolic, biochemical and electric signals. In addition, beyond their role in direct intercellular communication, Cxs also form unapposed, non-junctional hemichannels in the plasma membrane that allow the passage of several paracrine messengers, complementing direct GJ communication. Within the NGVU, Cxs are expressed in vascular endothelial cells, including those that form the BBB, and are eminent in astrocytes, especially at their endfoot processes that wrap around cerebral vessels. However, despite the density of Cx channels at this so-called gliovascular interface, it remains unclear as to how Cx-based signaling between astrocytes and BBB endothelial cells may converge control over BBB permeability in health and disease. In this review we describe available evidence that supports a role for astroglial as well as endothelial Cxs in the regulation of BBB permeability during development as well as in disease states.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/8529725Test; http://hdl.handle.net/1854/LU-8529725Test; http://dx.doi.org/10.1007/s11064-017-2313-xTest; https://biblio.ugent.be/publication/8529725/file/8544198Test

الإتاحة: https://doi.org/10.1007/s11064-017-2313-xTest
https://biblio.ugent.be/publication/8529725Test
http://hdl.handle.net/1854/LU-8529725Test
https://biblio.ugent.be/publication/8529725/file/8544198Test

المؤلفون: De Bock, Marijke, Decrock, Elke, Wang, Nan, Bol, Mélissa, Vinken, Mathieu, Bultynck, Geert, Leybaert, Luc

المصدر: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH ; ISSN: 0167-4889

مصطلحات موضوعية: Medicine and Health Sciences, Connexin, Ca2+, Intercellular communication, Neuro-glio-vascular unit, Astrocyte, Neurodegeneration, SPINAL-CORD-INJURY, INTERCELLULAR CALCIUM WAVES, GAP-JUNCTIONAL COMMUNICATION, NECROSIS-FACTOR-ALPHA, CYCLIC ADP-RIBOSE, SPONTANEOUS EPILEPTIFORM ACTIVITY, CA2+-DEPENDENT GLUTAMATE RELEASE, EXTRASYNAPTIC NMDA RECEPTORS, HIPPOCAMPAL SLICE CULTURES, PANNEXIN 1 CHANNELS

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/5829813Test; http://hdl.handle.net/1854/LU-5829813Test; http://dx.doi.org/10.1016/j.bbamcr.2014.04.016Test; https://biblio.ugent.be/publication/5829813/file/6834756Test

الإتاحة: https://doi.org/10.1016/j.bbamcr.2014.04.016Test
https://biblio.ugent.be/publication/5829813Test
http://hdl.handle.net/1854/LU-5829813Test
https://biblio.ugent.be/publication/5829813/file/6834756Test

المؤلفون: Crunelli, Vincenzo, Blethyn, Kate L., Cope, David W., Hughes, Stuart W., Parri, H. Rheinallt, Turner, Jonathan P., Toth, Tibor I., Williams, Stephen R.

مصطلحات موضوعية: Lateral Geniculate-Nucleus, Low-Frequency Rhythms, Thalamic Relay Cells, Gap-Junctional Communication, Dependent Glutamate Release, Less-Than-1 Hz Oscillation, Central-Nervous-System, In-Vitro, Calcium Waves, Rat-Brain

الوصف: In this review, we summarize three sets of findings that have recently been observed in thalamic astrocytes and neurons, and discuss their significance for thalamocortical loop dynamics.(i) A physiologically relevant 'window' component of the low-voltage-activated, T-type Ca2+ current (I-Twindow) plays an essential part in the slow (less than 1 Hz) sleep oscillation in adult thalamocortical (TC) neurons, indicating that the expression of this fundamental sleep rhythm in these neurons is not a simple reflection of cortical network activity, It is also likely that I-Twindow underlies one of the cellular mechanisms enabling TC neurons to produce burst firing in response to novel sensory stimuli.(ii) Both electrophysiological and dye-injection experiments support the existence of gap junction-mediated coupling among young and adult TC neurons. This finding indicates that electrical coupling-mediated synchronization might be implicated in the high and low frequency oscillatory activities expressed by this type of thalamic neuron.(iii) Spontaneous intracellular Ca2+ ([Ca2+],) waves propagating among thalamic astrocytes are able to elicit large and long-lasting X-methyl-D-aspartate-mediated currents in TC neurons. The peculiar developmental profile within the first two postnatal weeks of these astrocytic [Ca2+], transients and the selective activation of these glutamate receptors point to a role for this astrocyte-to-neuron signalling mechanism in the topographic wiring of the thalamocortical loop. As some of these novel cellular and intracellular properties are not restricted to thalamic astrocytes and neurons, their significance may well apply to (patho) physiological functions of glial and neuronal elements in other brain areas.

العلاقة: orcid:0000-0001-5352-7947

الإتاحة: https://doi.org/10.1098/rstb.2002.1155Test
https://espace.library.uq.edu.au/view/UQ:6be8c39Test

المؤلفون: Liu, Tao, Sun, Lei, Xiong, Yingfei, Shang, Shujiang, Guo, Ning, Teng, Sasa, Wang, Yeshi, Liu, Bin, Wang, Changhe, Wang, Li, Zheng, Lianghong, Zhang, Claire Xi, Han, Weiping, Zhou, Zhuan

المساهمون: Zhang, CX (reprint author), Peking Univ, Inst Mol Med, State Key Lab Biomembrane & Membrane Biotechnol, 5 Yiheyuan Rd, Beijing 100871, Peoples R China., Peking Univ, Inst Mol Med, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100871, Peoples R China., Agcy Sci Technol & Res, Singapore Bioimaging Consortium, Lab Metab Med, Singapore 138667, Singapore., Peking Univ, Inst Mol Med, State Key Lab Biomembrane & Membrane Biotechnol, 5 Yiheyuan Rd, Beijing 100871, Peoples R China.

المصدر: PubMed ; SCI

مصطلحات موضوعية: DEPENDENT GLUTAMATE RELEASE, LONG-TERM POTENTIATION, SYNAPTIC VESICLES, VESICULAR COMPARTMENT, REGULATED EXOCYTOSIS, LYSOSOMAL EXOCYTOSIS, CA2+ SENSOR, D-SERINE, RECEPTOR, FUSION

الوصف: Astrocytes release a variety of signaling molecules including glutamate, D-serine, and ATP in a regulated manner. Although the functions of these molecules, from regulating synaptic transmission to controlling specific behavior, are well documented, the identity of their cellular compartment(s) is still unclear. Here we set out to study vesicular exocytosis and glutamate release in mouse hippocampal astrocytes. We found that small vesicles and lysosomes coexisted in the same freshly isolated or cultured astrocytes. Both small vesicles and lysosome fused with the plasma membrane in the same astrocytes in a Ca2+-regulated manner, although small vesicles were exocytosed more efficiently than lysosomes. Blockade of the vesicle glutamate transporter or cleavage of synaptobrevin 2 and cellubrevin (both are vesicle-associated membrane proteins) with a clostridial toxin greatly inhibited glutamate release from astrocytes, while lysosome exocytosis remained intact. Thus, both small vesicles and lysosomes contribute to Ca2+-dependent vesicular exocytosis, and small vesicles support glutamate release from astrocytes. ; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000292921100018&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Test ; Neurosciences ; SCI(E) ; PubMed ; 47 ; ARTICLE ; 29 ; 10593-10601 ; 31

العلاقة: JOURNAL OF NEUROSCIENCE.2011,31,(29),10593-10601.; 662539; http://hdl.handle.net/20.500.11897/343642Test; WOS:000292921100018

الإتاحة: https://doi.org/20.500.11897/343642Test
https://doi.org/10.1523/JNEUROSCI.6401-10.2011Test
https://hdl.handle.net/20.500.11897/343642Test

المؤلفون: Chen, Xiao-Ke, Xiong, Ying-Fei, Zhou, Zhuan

المساهمون: Zhou, Z (reprint author), Peking Univ, Inst Mol Med, 5 Yi He Yuan Rd, Beijing 100871, Peoples R China., Peking Univ, Inst Mol Med, Beijing 100871, Peoples R China., Peking Univ, Coll Life Sci, State Key Lab Biomembrane Engn, Beijing 100871, Peoples R China., Fourth Mil Med Univ, Inst Neurosci, Xian 710032, Peoples R China., Peking Univ, Inst Mol Med, 5 Yi He Yuan Rd, Beijing 100871, Peoples R China.

المصدر: PubMed ; SCI

مصطلحات موضوعية: kiss-and-run, fusion pore, amperometry, glutamate, astrocyte, ADRENAL CHROMAFFIN CELLS, DEPENDENT GLUTAMATE RELEASE, SINGLE SYNAPTIC VESICLES, CALCIUM OSCILLATIONS, TRANSMITTER RELEASE, QUANTAL SECRETION, MEMBRANE-FUSION, GROWTH CONES, IN-SITU, NEURONS

الوصف: Neurons and glia are the principal cellular components of the nervous system. Although the glia are 10 times more numerous than neurons, until recently they were thought to be passive cells that monitor and support the active neurons by taking up used neurotransmitters from the synapses. In the past few years, this concept has been challenged by the findings that Ca2+ waves spread from one astrocyte to another via Ca2+- and SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor)-dependent gliotransmitter release in pure cultures of astrocytes, raising the possibility that glia are not so passive as previously thought. This hypothesis was further advanced by two recent reports, which demonstrated that astrocytes release glutamate via vesicular exocytosis in response to stimuli. The kinetics of single vesicle exocytosis is distinct from its neural equivalent, because in response to physiological stimulation, gliotransmitter release is exclusively in the mode of "kiss and run." These advances were made possible by newly available techniques for single vesicle recordings, which will also be briefly reviewed here. ; Clinical Neurology ; Neurosciences ; SCI(E) ; PubMed ; 5 ; ARTICLE ; 5 ; 375-378 ; 12

العلاقة: NEUROSCIENTIST.2006,12,(5),375-378.; 674518; http://hdl.handle.net/20.500.11897/345765Test; WOS:000240679400009

الإتاحة: https://doi.org/20.500.11897/345765Test
https://doi.org/10.1177/1073858406291588Test
https://hdl.handle.net/20.500.11897/345765Test

المؤلفون: Braet, Katleen, Cabooter, Liesbet, Paemeleire, Koen, Leybaert, Luc

مصطلحات موضوعية: Cell Biology, neuron, glial cell, brain vessel, neuroglial, neurovascular, gliovascular, neurobarrier, gap junctions, paracrine signaling, Cultured Hippocampal-neurons, Dependent Glutamate Release, Spinal-cord Astrocytes, Methyl-d-aspartate, Cyclic Adp-ribose, Gap Junctional Communication, Platelet-activating-factor, Intercellular Ca2+ Waves, Brain Endothelial-cells, Long-term Potentiation, 03 Chemical Sciences, 0304 Medicinal and Biomolecular Chemistry

الوصف: The communication of calcium signals between cells is known to be operative between neurons where these signals integrate intimately with electrical and chemical signal communication at synapses. Recently, it has become clear that glial cells also exchange calcium signals between each other in cultures and in brain slices. This communication pathway has received utmost attention since it is known that astrocytic calcium signals can be induced by neuronal stimulation and can be communicated back to the neurons to modulate synaptic transmission. In addition to this, cells that are generally not considered as brain cells become progressively incorporated in the picture, as astrocytic calcium signals are reported to be communicated to endothelial cells of the vessel wall and can affect smooth muscle cell tone to influence the vessel diameter and thus blood flow. We review the available evidence for calcium signal communication in the central nervous system, taking into account a basic functional unit -the brain cell tripartite- consisting of neurons, glial cells and vascular cells and with emphasis on glial-vascular calcium signaling aspects. (C) 2003 Elsevier SAS. All rights reserved.

الإتاحة: https://doi.org/10.1016/j.biolcel.2003.10.007Test
https://espace.library.uq.edu.au/view/UQ:122975Test

المؤلفون: Rossi, D., Volterra, A.

المصدر: Brain Research Bulletin, vol. 80, no. 4-5, pp. 224-232

مصطلحات موضوعية: Astrocyte, Calcium, Glutamate, AIDS dementia complex, Alzheimer's disease, Amyotrophic lateral sclerosis, Amyotrophic-Lateral-Sclerosis, Motor-Neuron Degeneration, Dependent Glutamate Release, Cu,Zn Superoxide-Dismutase, Alzheimers-Disease Brain, Necrosis-Factor-Alpha, Fibrillary Acidic Protein, Predict Cognitive Status, Central-Nervous-System, Glial Tnf-Alpha

الوصف: For decades, astrocytes have been regarded as passive partners of neurons in central nervous system (CNS) function. Studies of the last 20 years, however, challenged this view by demonstrating that astrocytes possess functional receptors for neurotransmitters and respond to their stimulation via release of gliotransmitters, including glutamate. Notably, astrocytes react to synaptically released neurotransmitters with intracellular calcium ([Ca(2+)]) elevations, which result in the release of glutamate via regulated exocytosis and, possibly, other mechanisms. These findings have led to a new concept of neuron-glia intercommunication where astrocytes play an unsuspected dynamic role by integrating neuronal inputs and modulating synaptic activity. The additional observation that glutamate release from astrocytes is controlled by molecules linked to inflammatory reactions, such as the cytokine tumor necrosis factor alpha (TNFalpha) and prostaglandins (PGs), suggests that glia-to-neuron signalling may be sensitive to changes in the production of these mediators occurring in pathological conditions. Indeed, a local, parenchymal brain inflammatory reaction (neuroinflammation) characterized by astrocytic and microglial activation has been reported in several neurodegenerative disorders, including AIDS dementia complex, Alzheimer's disease and amyotrophic lateral sclerosis. This transition may be accompanied by functional de-regulation and even degeneration of the astrocytes with the consequent disruption of the cross-talk normally occurring between these cells and neurons. Incorrect neuron-astrocyte interactions may be involved in neuronal derangement and contribute to disease development. The findings reported in this review suggest that a better comprehension of the glutamatergic interplay between neurons and astrocytes may provide information about normal brain function and also highlight potential molecular targets for therapeutic interventions in pathology.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/19631259; info:eu-repo/semantics/altIdentifier/pissn/1873-2747[electronic]; https://serval.unil.ch/notice/serval:BIB_45382C0454FDTest

الإتاحة: https://doi.org/10.1016/j.brainresbull.2009.07.012Test
https://serval.unil.ch/notice/serval:BIB_45382C0454FDTest