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1مؤتمر
المؤلفون: Chabi, Béatrice, Ost, Mario, Gama-Perez, Pau, Dahdah, Nora, Lemieux, Hélène, Holody, Claudia, Rg, Carpenter, Tepp, Kersti, Puurand, Marju, Kaambre, Tuuli, Dubouchaud, Hervé, Cortade, Fabienne, Pesta, Dominik, Calabria, Elisa, Casado, Marta, Pinna, Fernandez-Ortiz, Marisol, Acuña-Castroviejo, Dario, Villena, Josep, A, Grefte, Sander, Keijer, Jaap, O'Brien, Kristin, Sowton, Alice, Murray, Andrew, J, Campbell, Matthew, D, Marcinek, David, J, Nollet, Edgar, Wüst, Rob, C, Dayanidhi, Sudarshan, Gnaiger, Erich, Doerrier, Carolina, Garcia-Roves, Pablo, M
المساهمون: Dynamique Musculaire et Métabolisme (DMEM), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM), German Institute of Human Nutrition Potsdam-Rehbrücke (DIfE), Institut d'Investigació Biomèdica de Bellvitge = Bellvitge Biomedical Research Institute (IDIBELL), University of Alberta, National Institute of Chemical Physics and Biophysics = Keemilise ja bioloogilise füüsika instituut Estonie (NICPB, Laboratory of Fundamental and Applied Bioenergetics = Laboratoire de bioénergétique fondamentale et appliquée (LBFA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 ), German Diabetes Center, Università degli studi di Verona = University of Verona (UNIVR), Instituto de biomedicina Valencia (IBV), Consejo Superior de Investigaciones Cientificas España = Spanish National Research Council Spain (CSIC), Universidad de Granada = University of Granada (UGR), Vall d’Hebron Research Institute (VHIR), Human and Animal Physiology Wageningen, The Netherlands, Wageningen University and Research Wageningen (WUR), University of Cambridge UK (CAM), Department of Radiology, University of Washington, Faculty of Behavioural and Movement Sciences Amsterdam, The Netherlands, Rehabilitation Institute of Chicago (RIC), Rehabilitation Institute of Chicago USA, Oroboros Instruments, Mitochondrial Physiology Society
المصدر: MitoFit Preprint Archives ; MiP2019/MitoEAGLE - 14th Conference on Mitochondrial Physiology: Mitochondrial function: changes during life cycle and in noncommunicable diseases ; https://hal.inrae.fr/hal-02941367Test ; MiP2019/MitoEAGLE - 14th Conference on Mitochondrial Physiology: Mitochondrial function: changes during life cycle and in noncommunicable diseases, Mitochondrial Physiology Society, Oct 2019, Belgrade, Serbia. 3p., ⟨10.26124/mitofit:ea19.MitoEAGLE.0001⟩ ; https://www.mitoglobal.org/index.php/MiP2019/MitoEAGLE_Belgrade_RSTest
مصطلحات موضوعية: mitochondrial respiration, [SDV]Life Sciences [q-bio]
جغرافية الموضوع: Serbia
الوقت: Belgrade, Serbia
الوصف: International audience
العلاقة: hal-02941367; https://hal.inrae.fr/hal-02941367Test; https://hal.inrae.fr/hal-02941367/documentTest; https://hal.inrae.fr/hal-02941367/file/2019_Chabi_MitoFit_Preprint_Arch_doi_10.26124mitofitea19.mitoeagle.0001.pdfTest
الإتاحة: https://doi.org/10.26124/mitofit:ea19.MitoEAGLE.0001Test
https://hal.inrae.fr/hal-02941367Test
https://hal.inrae.fr/hal-02941367/documentTest -
2دورية أكاديمية
المؤلفون: Pessemesse, Laurence, Tintignac, Lionel, Blanchet, Emilie, Cortade, Fabienne, Jublanc, Elodie, Demangel, Rémi, Py, Guillaume, Sar, Chamroeun, Cabello, Gerard, Cabello, Chantal, Casas, François
المساهمون: Dynamique Musculaire et Métabolisme (DMEM), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM), Pharmazentrum, Neuromuscular Res Ctr, Dept Neurol, Dept Biomed, Université de Bâle = University of Basel = Basel Universität (Unibas), Institut National de la Recherche Agronomique (INRA), Institut des Neurosciences de Montpellier (INM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)
المصدر: ISSN: 2045-2322.
مصطلحات موضوعية: thyroid hormone, t3 receptor, myoblast differentiation, overexpression, proliferation, expression, plays, [SDV]Life Sciences [q-bio]
الوصف: Thyroid hormone is a major regulator of skeletal muscle development and repair, and also a key regulator of mitochondrial activity. We have previously identified a 43 kDa truncated form of the nuclear T3 receptor TR alpha 1 (p43) which stimulates mitochondrial activity and regulates skeletal muscle features. However, its role in skeletal muscle regeneration remains to be addressed. To this end, we performed acute muscle injury induced by cardiotoxin in mouse tibialis in two mouse models where p43 is overexpressed in or depleted from skeletal muscle. The measurement of muscle fiber size distribution at different time point (up to 70 days) upon injury lead us to unravel requirement of the p43 signaling pathway for satellite cells dependent muscle regeneration; strongly delayed in the absence of p43; whereas the overexpression of the receptor enhances of the regeneration process. In addition, we found that satellite cells derived from p43-Tg mice display higher proliferation rates when cultured in vitro when compared to control myoblasts, whereas p43-/- satellites shows reduced proliferation capacity. These finding strongly support that p43 plays an important role in vivo by controling the duration of skeletal muscle regeneration after acute injury, possibly through the regulation of mitochondrial activity and myoblasts proliferation.
العلاقة: info:eu-repo/semantics/altIdentifier/pmid/31439911; hal-02285621; https://hal.science/hal-02285621Test; https://hal.science/hal-02285621/documentTest; https://hal.science/hal-02285621/file/2019_Casas_ScientificReports.pdfTest; PRODINRA: 483518; PUBMED: 31439911; WOS: 000482182200024
الإتاحة: https://doi.org/10.1038/s41598-019-48703-2Test
https://hal.science/hal-02285621Test
https://hal.science/hal-02285621/documentTest
https://hal.science/hal-02285621/file/2019_Casas_ScientificReports.pdfTest -
3مؤتمر
المؤلفون: Cortade, Fabienne, Gaillard, Virginie, Chabi, Béatrice
المساهمون: Dynamique Musculaire et Métabolisme (DMEM), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM), Institut du Temps Géré
المصدر: MITOEAGLE 2017 ; https://hal.inrae.fr/hal-02941628Test ; MITOEAGLE 2017, Jul 2017, Obergurgl, Austria. , 2017 ; https://wiki.oroboros.at/index.php/MitoEAGLE_Obergurgl_2017Test
مصطلحات موضوعية: [SDV]Life Sciences [q-bio]
الوصف: International audience ; To optimize the permeabilized fibers (pf) preparation from mouse Tibialis anterior in our lab, we used the Design of Experiments (DoE) methodology thatevaluates the impact of 6 experimental conditions or factors, on the pf respiration parameters (Pyruvate Malate Succinate respiration (PMS leak) andrespiratory control ratio (RCRPMS)), to provide a maximum of information using a limited number of experiments and animals.
العلاقة: hal-02941628; https://hal.inrae.fr/hal-02941628Test; https://hal.inrae.fr/hal-02941628/documentTest; https://hal.inrae.fr/hal-02941628/file/2017_Chabi_Poster%20COST%20MITOEAGLE%202017%20final.pdfTest
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4دورية أكاديمية
المؤلفون: Britto, Florian, A, Cortade, Fabienne, Belloum, Yassine, Blaquière, Marine, Gallot, Yann, Docquier, Aurélie, Pagano, Allan, Jublanc, Elodie, Bendridi, Nadia, Koechlin-Ramonatxo, Christelle, Chabi, Béatrice, Francaux, Marc, Casas, François, Freyssenet, Damien, Rieusset, Jennifer, Giorgetti-Peraldi, Sophie, Carnac, Gilles, Ollendorff, Vincent, Favier, François Bertrand
المساهمون: Dynamique Musculaire et Métabolisme (DMEM), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM), Physiologie & médecine expérimentale du Cœur et des Muscles U 1046 (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry ), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Catholique de Louvain = Catholic University of Louvain (UCL), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)
المصدر: ISSN: 1741-7007.
مصطلحات موضوعية: mTOR, Mitochondria, Energy expenditure, Exercise, Skeletal muscle, Metabolism, MAMs, Hypoxia, Fasting, [SDV]Life Sciences [q-bio]
الوصف: International audience ; Skeletal muscle atrophy is a common feature of numerous chronic pathologies and is correlated with patient mortality. The REDD1 protein is currently recognized as a negative regulator of muscle mass through inhibition of the Akt/mTORC1 signaling pathway. REDD1 expression is notably induced following glucocorticoid secretion, which is a component of energy stress responses.
العلاقة: info:eu-repo/semantics/altIdentifier/pmid/29895328; hal-01815521; https://hal.umontpellier.fr/hal-01815521Test; https://hal.umontpellier.fr/hal-01815521/documentTest; https://hal.umontpellier.fr/hal-01815521/file/Favier_01.pdfTest; PRODINRA: 432910; PUBMED: 29895328; WOS: 000435459200001
الإتاحة: https://doi.org/10.1186/s12915-018-0525-4Test
https://hal.umontpellier.fr/hal-01815521Test
https://hal.umontpellier.fr/hal-01815521/documentTest
https://hal.umontpellier.fr/hal-01815521/file/Favier_01.pdfTest -
5دورية أكاديمية
المؤلفون: FAVIER, Francois, Britto, Florian, Belloum, Yassine, Gallot, Yann, Cortade, Fabienne, Chabi, Beatrice, Freyssenet, Damien, Carnac, Gilles, Rieusset, Jennifer, Giorgetti‐Peraldi, Sophie, Ollendorff, Vincent
المساهمون: Agence Française de Lutte contre le Dopage
المصدر: The FASEB Journal ; volume 32, issue S1 ; ISSN 0892-6638 1530-6860
الوصف: Aims and background Regulated in Development and DNA Damage 1 (REDD1) is a stress‐induced protein responsible for the inhibition of the Akt/mTORC1 pathway. This pathway integrates energetic status and oxygen availability to promote protein and glycogen synthesis as well as mitochondrial biogenesis. REDD1 is expressed in response to exercise, hypoxia or fasting and has been observed in the mitochondrial fraction in vitro. Our aim is to characterize the role of REDD1 in skeletal muscle under energetic challenge. Methods and results Wild‐type (WT) and REDD1 knockout (KO) mice were deprived of food for 16h and immediately killed before gastrocnemius and tibialis anterior muscles sampling. We observed an overactivation of the metabolic sensor AMPK and a greater glycogen depletion in skeletal muscle of REDD1 KO mice after fasting. In addition, REDD1 deficient mice displayed exacerbation of skeletal muscle atrophy in response to food deprivation compared to WT animals. We then used dexamethasone treatment to induce REDD1 protein expression and showed for the first time that REDD1 is (partly) localized at the mitochondrial‐associated endoplasmic reticulum membranes (MAM) of skeletal muscle fibers, where it is able to inhibit the Akt/mTORC1 pathway. Indeed, REDD1 binds to and reduces the interaction of MAM protein components, resulting in decreased mitochondrial O 2 consumption and protein synthesis. Conclusions Sustained activity of the Akt/mTORC1 pathway is known to promote anabolic processes and therefore to increase ATP consumption. Our results supports that REDD1 deletion contribute to maintain high fuel demand that in turn leads to skeletal muscle atrophy under fasting. We propose here that a physiological role for REDD1 is to foster skeletal muscle acclimatization during energetic challenges via reduction of O2 and ATP consumption assigned to synthesis processes. Support or Funding Information This work was supported by the Agence Française de Lutte contre le Dopage (grant #4299). This abstract is from the ...
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6مؤتمر
المؤلفون: Britto, Florian, Belloum, Yassine, Ramonatxo, Christelle, Jublanc, Elodie, Cortade, Fabienne, Chabi, Béatrice, Freyssenet, Damien, Giorgetti-Peraldi, Sophie, Ollendorff, Vincent, Favier, François Bertrand
المساهمون: Dynamique Musculaire et Métabolisme (DMEM), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry ), Mediterranean Research Centre for Molecular Medicine, Institut National de la Santé et de la Recherche Médicale (INSERM)
المصدر: 45. European Muscle Conference
https://hal.science/hal-01837649Test
45. European Muscle Conference, Sep 2016, Montpellier, France. 1 pمصطلحات موضوعية: mice, souris, synthèse de protéine, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
جغرافية الموضوع: Montpellier, France
الوصف: Does REDD1 act as an AMPK-like in skeletal muscle ?. 45. European Muscle Conference
العلاقة: hal-01837649; https://hal.science/hal-01837649Test; https://hal.science/hal-01837649/documentTest; https://hal.science/hal-01837649/file/36DED600-0834-40B3-B7E0-A0E4C5044E5A_1Test; https://hal.science/hal-01837649/file/2016_Britto_EMC_2Test; PRODINRA: 370696
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7مؤتمر
المؤلفون: Chabi, Béatrice, Fouret, Gilles, Lecomte, Jérôme, Pessemesse, Laurence, Cortade, Fabienne, Coudray, Charles, Casas, François, Wrutniak Cabello, Chantal, Feuillet-Coudray, Christine
المساهمون: Dynamique Musculaire et Métabolisme (DMEM), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM), Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)
المصدر: Journées Francophones de Nutrition ; https://hal.science/hal-01837653Test ; Journées Francophones de Nutrition, Nov 2016, Montpellier, France. 221 p., 2016, Journées francophones de nutrition
مصطلحات موضوعية: voluntary muscle, membrane mitochondriale, muscle squelettique, cardiolipide, respiration mitochondriale, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
جغرافية الموضوع: Montpellier, France
الوصف: Introduction et but de l’étude : Des travaux récents indiquent que la fonctionnalité de la mitochondrie peut être influencée par la composition en lipides des membranes mitochondriales, en particulier le cardiolipide, un phospholipide exclusivement présent dans la membrane mitochondriale. La teneur en cardiolipide impacte la fluidité membranaire, l’organisation et le fonctionnement des complexes de la chaîne respiratoire, et la production mitochondriale d’espèces oxygénées réactives.Sirt3, une des 7 protéines de la famille des sirtuines, est une déacétylase localisée principalement dans la mitochondrie. Elle déacétyle les résidus lysine de protéines impliquées dans l’oxydation des lipides, le fonctionnement de la chaîne respiratoire, et les défenses antioxydantes, et participe ainsi à la régulation du métabolisme lipidique, de la synthèse d’ATP et du stress oxydant. Récemment, deux isoformes de Sirt3, mitochondriale (Sirt3-M1) et cytosolique (Sirt3-M3), ont été mises en évidence.Afin d’étudier l’impact de Sirt3 sur la composition lipidique de la mitochondrie et sa fonctionnalité dans muscle, nous avons étudié la composition en phospholipides et en acide gras des mitochondries de souris surexprimant chacune des isoformes de Sirt3 spécifiquement dans le muscle et mesuré la respiration et l’activité des complexes mitochondriaux.Matériel et méthodes : Un groupe de souris (5-6 mois) WT (n=6-8) et deux groupes de souris surexprimant Sirt3 SIRT3- M1 (n=7-8) ou SIRT3-M3 (n=5-6) ont été étudiés. La composition en phospholipides du muscle ou des mitochondries de muscle a été déterminée par CCM, et la composition en acide gras par CPG. La respiration mitochondriale a été mesurée par oxygraphie haute résolution et l’activité des complexes de la chaine respiratoire mitochondriale a été évaluée par spectrophotométrie.Résultats et Analyse statistique : Alors que la composition en lipides du muscle n’est pas modifiée quelques soient les groupes étudiés, nos travaux montrent que la composition en phospholipides et en acide gras ...
العلاقة: hal-01837653; https://hal.science/hal-01837653Test; https://hal.science/hal-01837653/documentTest; https://hal.science/hal-01837653/file/Chabi-JFN-2016_1.pdfTest; PRODINRA: 381179
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8مؤتمرCommitment to reproducibility in mitochondrial respiration studies with permeabilized muscle fibers.
المؤلفون: Doerrier, Carolina, Gama Perez, Pau, Chabi, Béatrice, Mario, Ost, Distefano, Giovanna, Pesta, Dominik, Dahdah Norma, Ramon, Lemieux, Helene, Holody, Claudia, Rg, Carpenter, Tepp, Kersti, Puurand, Marju, Käämbre, Tuuli, Dubouchaud, Herve, Cortade, Fabienne
المساهمون: Oroboros Instruments, University of Barcelona, Dynamique Musculaire et Métabolisme (DMEM), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université de Montpellier (UM), University Hospital Leipzig = Universitätsklinikum Leipzig, Florida Hospital, DLR Institute of Aerospace Medicine, Deutsches Zentrum für Luft- und Raumfahrt Köln (DLR), University of Alberta, National Institute of Chemical Physics and Biophysics = Keemilise ja bioloogilise füüsika instituut Estonie (NICPB, Laboratory of Bioenergetics, BrainTech Laboratory CHU Grenoble Alpes - Inserm U1205 (Brain Tech Lab ), Centre Hospitalier Universitaire CHU Grenoble (CHUGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA)
المصدر: 2020 Padua Muscle Days Padova IT
https://hal.inrae.fr/hal-04233642Test
2020 Padua Muscle Days Padova IT, Nov 2020, Padova, Italy.
https://wiki.oroboros.at/index.php/Doerrier_2020_PaduaMuscleDays_PosterTestمصطلحات موضوعية: Mitochondria, mitochondrial database, mitochondrial respiration, reproducibility crisis, permeabilized muscle fibers, reference values, [SDV]Life Sciences [q-bio]
جغرافية الموضوع: Italy
الوقت: Padova, Italy
الوصف: International audience ; The exponential increase of scientific publications in the mitochondrial field shows the growing interest in mitochondria. However, the lack of methodological consistency in many published projects on mitochondrial respiratory function complicates a quantitative inter/intra-laboratory comparison of datasets. This deficiency manifests the need to improve the quality in science[1]. In this context, the MitoEAGLE COST Action is a powerful framework committed to evaluate and enhance the reproducibility in mitochondrial physiology as a basis to establish a novel mitochondrial database related to Evolution, Age, Gender, Lifestyle and Environment. Permeabilized muscle fibers are widely used to evaluate mitochondrial function in health and disease[2]. Therefore, our main goals are to: 1) compare protocols used in different research laboratories, 2) analyze factors which contribute to experimental variability, 3) define optimal experimental conditions in muscle studies, 4) elaborate guidelines for evaluating mitochondrial function in muscle tissue, 5) establish reference values on mitochondrial respiration, particularly as a test of the skills in preparing high-quality permeabilized muscle fibers, and 6) generate a database. To achieve our aims, two unique studies are currently in progress: 1) 17 international research groups performing independently experiments on respiration in permeabilized fibers of mouse soleus muscle, following the same experimental procedure[3]; 2) a blinded international study measuring simultaneously in the same laboratory respiration of permeabilized human skeletal fibers by high-resolution respirometry and assessing the effect of different experimental conditions[4]. Our results contribute to face the reproducibility crisis and provide the basis for establishing the first database on mitochondrial respiratory parameters in muscle tissues.
العلاقة: hal-04233642; https://hal.inrae.fr/hal-04233642Test
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9دورية أكاديمية
المساهمون: Dynamique Musculaire et Métabolisme (DMEM), Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Association Française contre les Myopathies
المصدر: ISSN: 1065-6995.
مصطلحات موضوعية: mitochondria, myogenic differentiation, respiratory complexes, ROS, [SDV.BC]Life Sciences [q-bio]/Cellular Biology
الوصف: International audience ; During myoblast differentiation, mitochondria undergo numerous changes that are necessary for the progression of the myogenic program. Notably, we previously showed that alteration in mitochondrial activity was able to control the expression of keys regulator of cell cycle withdrawal and terminal differentiation. Here, we assessed whether inhibition of one of the respiratory complexes was a key factor in the regulation of myogenic differentiation in C2C12 cells, and was associated with alteration in reactive oxygen species (ROS) production. C2C12 cells were treated from proliferation to differentiation with specific inhibitors of mitochondrial complexes at a concentration that were inhibiting respiration but not altering cell morphology. Proliferation was significantly repressed with inhibition of complexes I, II, and III, or mitochondrial protein synthesis (using Chloramphenicol treatment), while complex IV inhibition did not alter myoblast proliferation compared to control cells. Moreover, inhibition of complexes I and II altered cell cycle regulators, with p21 protein expression upregulated since proliferation and p27 protein expression reduced at differentiation. Myotubes formation and myogenin expression were blunted with complexes I and II inhibitors while MyoD protein expression was maintained, suggesting an alteration in its transcriptional activity. Finally, a decrease in overall ROS production was observed with continuous inhibition of mitochondrial complexes I-IV. In summary, our data provide evidence that complexes I and II may be the primary regulators of C2C12 myogenic differentiation. This occurs through specific regulation of myogenic rather than cell cycle regulators expression and ROS production at mitochondrial rather than cell level.
العلاقة: info:eu-repo/semantics/altIdentifier/pmid/33764610; hal-03201669; https://hal.inrae.fr/hal-03201669Test; PUBMED: 33764610; WOS: 000635694300001
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10دورية أكاديمية
المؤلفون: Abdel Khalek, Waed, Cortade, Fabienne, Ollendorff, Vincent, Lapasset, Laure, Tintignac, Lionel, Chabi, Béatrice, Cabello, Chantal
المساهمون: Dynamique Musculaire et Métabolisme (DMEM), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM), Institut de Génétique Moléculaire de Montpellier (IGMM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Biozentrum
المصدر: ISSN: 1932-6203.
مصطلحات موضوعية: [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis
الوصف: Sirtuin 3 (SIRT3), one of the seven mammalian sirtuins, is a mitochondrial NAD(+)-dependent deacetylase known to control key metabolic pathways. SIRT3 deacetylases and activates a large number of mitochondrial enzymes involved in the respiratory chain, in ATP production, and in both the citric acid and urea cycles. We have previously shown that the regulation of myoblast differentiation is tightly linked to mitochondrial activity. Since SIRT3 modulates mitochondrial activity, we decide to address its role during myoblast differentiation. For this purpose, we first investigated the expression of endogenous SIRT3 during C2C12 myoblast differentiation. We further studied the impact of SIRT3 silencing on both the myogenic potential and the mitochondrial activity of C2C12 cells. We showed that SIRT3 protein expression peaked at the onset of myoblast differentiation. The inhibition of SIRT3 expression mediated by the stable integration of SIRT3 short inhibitory RNA (SIRT3shRNA) in C2C12 myoblasts, resulted in: 1) abrogation of terminal differentiation - as evidenced by a marked decrease in the myoblast fusion index and a significant reduction of Myogenin, MyoD, Sirtuin 1 and Troponin T protein expression - restored upon MyoD overexpression; 2) a decrease in peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1 alpha) and citrate synthase protein expression reflecting an alteration of mitochondrial density; and 3) an increased production of reactive oxygen species (ROS) mirrored by the decreased activity of manganese superoxide dismutase (MnSOD). Altogether our data demonstrate that SIRT3 mainly regulates myoblast differentiation via its influence on mitochondrial activity.
العلاقة: hal-01135770; https://hal.science/hal-01135770Test; https://hal.science/hal-01135770/documentTest; https://hal.science/hal-01135770/file/Khalek-PlosOne-2014_%7B68F718AD-B85D-42B3-8672-913932956268%7D.pdfTest; PRODINRA: 286921; WOS: 000347515300034
الإتاحة: https://doi.org/10.1371/journal.pone.0114388Test
https://hal.science/hal-01135770Test
https://hal.science/hal-01135770/documentTest
https://hal.science/hal-01135770/file/Khalek-PlosOne-2014_%7B68F718AD-B85D-42B3-8672-913932956268%7D.pdfTest
