المؤلفون: Jörg Mey, Concepción García-Rama, Marta Aguado-Garrido, Enrique Pérez-Rizo, Lorenzo Romero-Ramírez, Boris Kramer

المصدر: IBRO Neuroscience Reports, Vol 15, Iss , Pp S188- (2023)

مصطلحات موضوعية: Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2667242123003482Test; https://doaj.org/toc/2667-2421Test

الوصول الحر: https://doaj.org/article/36161d33dc3049d3b6641ca019791becTest

المؤلفون: Lorenzo Romero-Ramírez, Concepción García-Rama, Jörg Mey

المصدر: IBRO Neuroscience Reports, Vol 15, Iss , Pp S289-S290 (2023)

مصطلحات موضوعية: Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2667242123005729Test; https://doaj.org/toc/2667-2421Test

الوصول الحر: https://doaj.org/article/c23de41cf9fc4de6bdf0c00f068d43d0Test

المؤلفون: Vinnitsa Buzoianu-Anguiano, Alejandro Arriero-Cabañero, Alfonso Fernandez-Mayoralas, Concepción García-Rama, Jörg Mey, Ernesto Doncel-Pérez

المصدر: IBRO Neuroscience Reports, Vol 15, Iss , Pp S169- (2023)

مصطلحات موضوعية: Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S266724212300307XTest; https://doaj.org/toc/2667-2421Test

الوصول الحر: https://doaj.org/article/a5e05d42311c42cabd0c8e664c47caceTest

المؤلفون: Marta Aguado-Garrido, Concepción García-Rama, Lorenzo Romero-Ramírez, Vinnitsa Buzoianu-Anguiano, Enrique Pérez-Rizo, Boris W. Kramer, Jörg Mey

المصدر: International Journal of Molecular Sciences, Vol 25, Iss 3, p 1548 (2024)

مصطلحات موضوعية: spinal cord injury, bone marrow, stromal cells, neuroinflammation, cytoprotection, Biology (General), QH301-705.5, Chemistry, QD1-999

الوصف: The treatment of spinal cord injury (SCI) with uncultivated human bone marrow-derived stromal cells (bmSCs) prepared by negative selection has been proposed to be therapeutically superior to treatment with stem cells that were expanded in vitro. To explore their use in clinical trials, we studied the functional effects of delayed application at 7 days after SCI by testing different doses of bmSCs. Spinal cord contusion injury was induced in adult male Wistar rats at the thoracic level T9. Human bmSCs were prepared by negative selection without expansion in vitro (NeuroCellsTM). Treatment consisted of one 150 µL injection into the cisterna magna containing 0.5 or 2.5 million fresh bmSCs or 2.5 million bmSCs. The recovery of motor functions was evaluated during a surveillance period of six weeks (6 W), during which spinal cords were assessed histologically. Treatment resulted in a significant, dose-dependent therapeutic effect on the recovery of motor performance. The histological analysis revealed a lower degree of axonal degeneration and better survival of neurons and oligodendrocytes in bmSCs treated rats. Our results support delayed intrathecal application of bmSCs prepared by negative selection without expansion in vitro as a treatment of SCI.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1422-0067/25/3/1548Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test

الوصول الحر: https://doaj.org/article/6e5e0e02c7994527a5579ec659a5fcf4Test

المؤلفون: María Gómez-Cañas, Paula Morales, Valentina Satta, Carmen Rodríguez-Cueto, Concepción García, Onintza Sagredo

المصدر: Frontiers in Neuroscience, Vol 16 (2022)

مصطلحات موضوعية: neurological diseases, cannabinoid, endocannabinoid system, neuroprotection, drug discovery, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fnins.2022.1108101/fullTest; https://doaj.org/toc/1662-453XTest

الوصول الحر: https://doaj.org/article/bd1cd1d358f8481384f31afa98f338d5Test

المؤلفون: Lorenzo Romero-Ramírez, Concepción García-Rama, Siyu Wu, Jörg Mey

المصدر: Scientific Reports, Vol 12, Iss 1, Pp 1-13 (2022)

مصطلحات موضوعية: Medicine, Science

الوصف: Abstract Glycolysis is the metabolic pathway that converts glucose into pyruvate. Central nervous system (CNS) pathologies, such as spinal cord injury (SCI) and ischemia, are accompanied by an increase of the glycolytic pathway in the damaged areas as part of the inflammatory response. Pyruvate kinase is a key glycolytic enzyme that converts phosphoenolpyruvate and ADP to pyruvate and ATP. The protein has two isoforms, PKM1 and PKM2, originated from the same gene. As a homodimer, PKM2 loses the pyruvate kinase activity and acts as a transcription factor that regulates the expression of target genes involved in glycolysis and inflammation. After SCI, resident microglia and hematogenous macrophages are key inducers of the inflammatory response with deleterious effects. Activation of the bile acid receptor TGR5 inhibits the pro-inflammatory NFκB pathway in microglia and macrophages. In the present study we have investigated whether bile acids affect the expression of glycolytic enzymes and their regulation by PKM2. Bacterial lipopolysaccharide (LPS) induced the expression of PKM1, PKM2 and its target genes in primary cultures of microglial and Raw264.7 macrophage cells. SCI caused an increase of PKM2 immunoreactivity in macrophages after SCI. Pretreatment with tauroursodeoxycholic acid (TUDCA) or taurolithocholic acid (TLCA) reduced the expression of PKM2 and its target genes in cell cultures. Similarly, after SCI, TUDCA treatment reduced the expression of PKM2 in the lesion center. These results confirm the importance of PKM2 in the inflammatory response in CNS pathologies and indicate a new mechanism of bile acids as regulators of PKM2 pathway.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-2322Test

الوصول الحر: https://doaj.org/article/4eb333749cc746d59a9d3ae02b3eed42Test

المؤلفون: Concepción García-Gómez, Rosa Ana Pérez, Beatriz Albero, Ana Obrador, Patricia Almendros, María Dolores Fernández

المصدر: Agronomy, Vol 13, Iss 8, p 2004 (2023)

مصطلحات موضوعية: combined toxicity, pesticides, degradation products, soil microorganisms, oxidative stress, photosynthetic pigments, Agriculture

الوصف: The use of zinc oxide nanoparticles (ZnO NPs), applied as a possible micronutrient source, in conjunction with organic pesticides in agricultural soils has the potential to alter the environmental behavior and toxicity of these chemicals to soil biota. This research examines the joint effects of ZnO NPs and the herbicide metribuzin (MTZ) on phytotoxicity to plants, toxicity to soil microorganisms, and the accumulation of Zn and MTZ in plants. After 23 days, effects on growth, photosynthetic pigment content, and oxidative stress biomarkers in bean plants (Phaseolus vulgaris) and soil enzymatic activities were evaluated. Additionally, the amounts of Zn and MTZ (and the latter’s main metabolites) in soil and plant tissues were quantified. ZnO NPs reduced ammonium oxidase activity and growth among MTZ-stressed plants while reducing photosynthetic pigment levels and enhancing antioxidant enzymatic activities. MTZ had a marginal impact on the availability and accumulation of Zn in plant tissues, although significant effects were observed in some specific cases. In turn, ZnO NPs drastically affected MTZ degradation in soil and influenced MTZ accumulation/metabolization in the bean plants. Our findings indicate that the indirect effects of ZnO NPs, through their interaction with commonly used organic pesticides, may be relevant and should be taken into account in agricultural soils.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2073-4395/13/8/2004Test; https://doaj.org/toc/2073-4395Test

الوصول الحر: https://doaj.org/article/bafda2864bf14d1e9949df7cb064ba49Test

المؤلفون: Solange Leda Houssay, Maricel Recalde, Marcela Giménez Rey, Concepción García, Raquel Urdaneta, Fernanda Huber, Claudia Flores, Carolina Domínguez, Emilio Nader

المصدر: Revista de la Sociedad Argentina de Diabetes, Vol 55, Iss 2, Pp 37-51 (2021)

مصطلحات موضوعية: hipoglucemia inadvertida, falla autonómica asociada a la hipoglucemia, hipoglucemia severa, diagnóstico, tratamiento., Nutritional diseases. Deficiency diseases, RC620-627, Diseases of the endocrine glands. Clinical endocrinology, RC648-665

الوصف: Hypoglycemia unawereness is a complication of type 1 diabetes treatment and of type 2 diabetes treatment treated with insulin or sulfonylureas, characterized by a reduced ability to perceive the onset of episodes of hypoglycemia. In general, it coexists with an insufficient counterregulatory hormonal response to hypoglycemia called: hypoglycemia associated autonomic failure (HAAF). The development of hypoglycemia unawereness and counterregulatory failure to hypoglycemia significantly increase the risk of severe hypoglycemia. Scoring scales have been developed to identify this group of people at high risk of severe hypoglycemia in the clinic. The cornerstone of treatment is to avoid hypoglycemia through a multifactorial intervention of clinical care and structured education.

وصف الملف: electronic resource

العلاقة: https://revistasad.com/index.php/diabetes/article/view/473Test; https://doaj.org/toc/0325-5247Test; https://doaj.org/toc/2346-9420Test

الوصول الحر: https://doaj.org/article/4bdd218a70334ce7a39fa7a4cf7989c2Test

المؤلفون: Concepción García Romero

المصدر: Tavira, Iss 21 (2022)

مصطلحات موضوعية: Language and Literature, Education

وصف الملف: electronic resource

العلاقة: https://revistas.uca.es/index.php/tavira/article/view/9403Test; https://doaj.org/toc/2792-9035Test

الوصول الحر: https://doaj.org/article/08872aa764e84977bf68493048183aadTest

المؤلفون: Concepción García Romero

المصدر: Tavira, Iss 20 (2022)

مصطلحات موضوعية: Language and Literature, Education

وصف الملف: electronic resource

العلاقة: https://revistas.uca.es/index.php/tavira/article/view/9390Test; https://doaj.org/toc/2792-9035Test

الوصول الحر: https://doaj.org/article/7cd3e1c1dfe84f71b3415edc3ce6a82fTest