المؤلفون: Rasheed, J K, Arko, R J, Feeley, J C, Chandler, F W, Thornsberry, C, Gibson, R J, Cohen, M L, Jeffries, C D, Broome, C V

المصدر: Infection and Immunity ; volume 47, issue 3, page 598-604 ; ISSN 0019-9567 1098-5522

الوصف: Staphylococcus aureus from patients with toxic shock syndrome (TSS) produce TSS toxin 1. We transferred, by a bacteriophage, the ability to produce TSS toxin 1 from a TSS toxin 1-positive to a TSS toxin 1-negative strain of S. aureus. This recombinant strain produced TSS toxin 1 as confirmed by isoelectric focusing, immunodiffusion, radioimmunoassay, and autoradiography. The recombinant produced TSS-like illness in rabbits, and was significantly (P less than 0.001) more lethal than the recipient strain. Both strains produced fever and diarrhea, but, in addition, rabbits challenged with the recombinant also developed lowered blood pressure (P = 0.002), conjunctival hyperemia, erythroderma, and respiratory distress. Histopathological findings in rabbits challenged with the recombinant strain were remarkably similar to those described for humans with TSS, e.g., erythrophagocytosis, liver "triaditis," and vasodilatation. This study demonstrates that this protein may contribute to the pathogenesis of the TSS.

الإتاحة: https://doi.org/10.1128/iai.47.3.598-604.1985Test

المؤلفون: Cohen, M L, Graves, L M, Hayes, P S, Gibson, R J, Rasheed, J K, Feeley, J C

المصدر: Journal of Clinical Microbiology ; volume 18, issue 2, page 372-375 ; ISSN 0095-1137 1098-660X

الوصف: Development of a new medium and modification of incubation conditions increased production of toxic shock syndrome marker proteins and enabled detection of small volumes of pyrogenic exotoxin C (PEC) by isoelectric focusing and staphylococcal enterotoxin F (SEF) by a newly developed solid-phase radioimmunoassay. The results were compared with those obtained with previously described methods. The results were identical, and all PEC-positive isolates were SEF positive. In a second study of 262 randomly selected Staphylococcus aureus isolates examined by isoelectric focusing and solid-phase radioimmunoassay but grown in fresh beef heart medium, 47 (17.9%) isolates were PEC and SEF positive; however, 9 (3.4%) were PEC positive and SEF negative, and 3 (1.1%) were SEF positive and PEC negative. When grown in buffered beef heart yeast extract medium, six of the previously PEC-positive and SEF-negative isolates were PEC negative. Autoradiographic analysis of selected isolates demonstrated that PEC- and SEF-positive strains bound SEF antitoxin to the protein at isoelectric point 7.2, suggesting that in staphylococci from patients with toxic shock syndrome, PEC and SEF are the same protein. In screening staphylococci for toxic shock syndrome marker proteins, isoelectric focusing to identify PEC may detect false-positive proteins and may be more susceptible to technical variation than immunological methods to detect SEF.

الإتاحة: https://doi.org/10.1128/jcm.18.2.372-375.1983Test

المؤلفون: Cohen, M L, Wong, E S, Falkow, S

المصدر: Antimicrobial Agents and Chemotherapy ; volume 21, issue 2, page 210-215 ; ISSN 0066-4804 1098-6596

الوصف: During a 7-month period in 1978 to 1979, 31 patients and personnel at a Kentucky hospital were colonized or infected with a Staphylococcus aureus strain resistant to clindamycin, erythromycin, gentamicin, methicillin, penicillin, and tetracycline. S. epidermidis with similar antibiotic resistance patterns had been isolated in this hospital in the year before the S. aureus outbreak. A 32-megadalton R-plasmid, pUW3626, mediating resistance to penicillin and gentamicin, was present in these isolates and in coisolated S. epidermidis from the same outbreak. By colony hybridization, pUW3626 was homologous to gentamicin R-plasmids from staphylococci isolated in other geographic areas. Our studies suggest that the emergency of antibiotic resistance in S. Aureus may result from genetic transfer from S. epidermidis as well as from the interhospital spread of resistant staphylococci.

الإتاحة: https://doi.org/10.1128/aac.21.2.210Test

المؤلفون: Hayes, P S, Graves, L M, Feeley, J C, Hancock, G A, Cohen, M L, Reingold, A L, Broome, C V, Hightower, A W

المصدر: Journal of Clinical Microbiology ; volume 20, issue 1, page 43-46 ; ISSN 0095-1137 1098-660X

الوصف: A total of 281 Staphylococcus aureus strains selected from those submitted to the Centers for Disease Control for phage typing between 1956 and 1982 were tested for the production of toxic-shock-associated protein (TSAP) by isoelectric focusing (IEF) and solid-phase radioimmunoassay. The results suggest that the observed temporal trends in the incidence of toxic-shock syndrome were not primarily due to changes in the distribution of TSAP-positive strains of S. aureus. Overall, 39 (14%) were TSAP positive by both methods. The earliest positive strain was an isolate submitted in 1957. TSAP-positive strains were most prevalent in the group of isolates submitted in 1976 for which 29% reacted, but the percent positive subsequently declined for isolates submitted in later years. TSAP production was more frequent among strains of phage types 29, 29/52, and 52 than among other strains. The use of IEF to identify TSAP detected false-positive proteins. Seven strains were positive by IEF and negative by solid-phase radioimmunoassay, whereas only one was positive by solid-phase radioimmunoassay and negative by IEF.

الإتاحة: https://doi.org/10.1128/jcm.20.1.43-46.1984Test

المؤلفون: Tacket, C O, Shahid, N, Huq, M I, Alim, A R, Cohen, M L

المصدر: Journal of Clinical Microbiology ; volume 20, issue 2, page 300-301 ; ISSN 0095-1137 1098-660X

الوصف: We studied the plasmid profiles of 136 Shigella isolates in Bangladesh to determine whether plasmid profiles could be used for differentiation of strains for epidemiological studies. Many different plasmid patterns were observed within each species, indicating that many genetically different strains of Shigella are responsible for illness in Bangladesh.

الإتاحة: https://doi.org/10.1128/jcm.20.2.300-301.1984Test

المؤلفون: COHEN, M. L., FALICOV, L. M.

المصدر: Science ; volume 243, issue 4890, page 547-547 ; ISSN 0036-8075 1095-9203

مصطلحات موضوعية: Multidisciplinary

الإتاحة: https://doi.org/10.1126/science.243.4890.547Test

المؤلفون: Bellamy, N., Bachmeier, C., Brooks, P. M., Browne, C. D., Cohen, M. L., March, L., Conaghan, P. G., Day, R. O., Campbell, J.

المصدر: InflammoPharmacology ; volume 5, issue 3, page 285-295 ; ISSN 0925-4692 1568-5608

مصطلحات موضوعية: Pharmacology (medical), Pharmacology, Immunology

الإتاحة: https://doi.org/10.1007/s10787-997-0006-9Test

المؤلفون: Smith, A., Weber, A., Pandher, Rimmie, Williams-Warren, Judy, Cohen, M. L., Ramsey, B.

المصدر: Infection ; volume 25, issue 2, page 106-108 ; ISSN 0300-8126 1439-0973

مصطلحات موضوعية: Infectious Diseases, Microbiology (medical), General Medicine

الإتاحة: https://doi.org/10.1007/bf02113587Test

المؤلفون: Suarez, J. I., Cohen, M. L., Larkin, J., Kernich, C. A., Hricik, D. E., Daroff, R. B.

المصدر: Neurology ; volume 48, issue 6, page 1678-1683 ; ISSN 0028-3878 1526-632X

الإتاحة: https://doi.org/10.1212/wnl.48.6.1678Test

المؤلفون: Knittle, E., Kaner, R. B., Jeanloz, R., Cohen, M. L.

المصدر: Physical Review B ; volume 51, issue 18, page 12149-12156 ; ISSN 0163-1829 1095-3795

الإتاحة: https://doi.org/10.1103/physrevb.51.12149Test