المؤلفون: De Simone S., Giacani E., Bosco M. A., Vittorio S., Ferrara M., Bertozzi G., Cipolloni L., La Russa R.

المساهمون: De Simone, S., Giacani, E., Bosco, M. A., Vittorio, S., Ferrara, M., Bertozzi, G., Cipolloni, L., La Russa, R.

مصطلحات موضوعية: miRNA, post-mortem investigation, postmortem miRNA, wound-age determination, wound-age estimation

الوصف: Background: The timing of wounds production is a significant issue in forensic pathology. Although various methods have been evaluated, obtaining an accurate dating of lesions is still a challenge. The pathologist uses many parameters to value wound age, such as histological and immunohistochemical. In recent years, there have been many studies regarding the use of miRNAs in wound-age estimation; indeed, miRNAs have multiple potential uses in forensic pathology. Scope: This review aims to verify the efficacy and feasibility of miRNAs as a tool for determining the timing of lesions. Materials and Methods: The authors conducted the systematic review according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. PubMed was used as a search engine to find articles published between January, 1st 2016 and October, 1st 2021, to evaluate the current state of the art regarding wound-age estimation. Results: A total of 256 articles were collected; after screening according to PRISMA guidelines, the systematic review included 8 articles. The studies included in this review were all Original articles evaluating the use of biomarkers for wound-age determination. Discussion and Conclusion: The literature review showed that analysis of miRNA is an innovative field of study with significant potentiality in forensic pathology. There are few studies, and almost all of them are at an early stage. The challenge is to understand how to standardize the samples' selection to obtain reliable experimental data. This observation represents a necessary prerequisite to planning further clinical trials.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/35096893; info:eu-repo/semantics/altIdentifier/wos/WOS:000758474300001; volume:8; firstpage:1; lastpage:9; numberofpages:9; journal:FRONTIERS IN MEDICINE; http://hdl.handle.net/11573/1649019Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85123940012

الإتاحة: https://doi.org/10.3389/fmed.2021.803067Test
http://hdl.handle.net/11573/1649019Test

المؤلفون: Ferrara M., Baldari B., Vittorio S., Bertozzi G., Cipolloni L., De Simone S.

المساهمون: Ferrara, M., Baldari, B., Vittorio, S., Bertozzi, G., Cipolloni, L., De Simone, S.

مصطلحات موضوعية: duty to rescue, penetrating heart wound, transcardiac injuries

الوصف: Penetrating cardiac injuries represent an increasingly frequent type of trauma in clinical and autopsy practice. The present study aims to review the specialist literature of the last decade (2010-2020) to assess whether the main features of these lesions have changed compared to previous years. The following characteristics were considered: Sex, age, cardiac structure involved, execution or not of surgery and postoperative survival, hemodynamic stability, circumstances and mechanism of production, injury and cause of death. Furthermore, the authors propose a practical appraisal of penetrating heart injury in which death was due to a delay in rescue. In line with the data obtained from the practical case, the review showed that compared to the past, the differences concern especially the mortality rate. This paper highlights that the forensic pathologist who approaches a case of transcardiac injury must consider that the circumstances of death are not always attributable to accidental events, attacks or suicides, but may also be due to clinical malpractice or failure to rescue.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/35090334; info:eu-repo/semantics/altIdentifier/wos/WOS:000766867900032; volume:27; issue:1; firstpage:1; lastpage:9; numberofpages:9; journal:FRONTIERS IN BIOSCIENCE; http://hdl.handle.net/11573/1649024Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85123875185

الإتاحة: https://doi.org/10.31083/j.fbl2701029Test
http://hdl.handle.net/11573/1649024Test

المؤلفون: Bertozzi G., Cafarelli F. P., Ferrara M., Di Fazio N., Guglielmi G., Cipolloni L., Manetti F., La Russa R., Fineschi V.

المساهمون: Bertozzi, G., Cafarelli, F. P., Ferrara, M., Di Fazio, N., Guglielmi, G., Cipolloni, L., Manetti, F., La Russa, R., Fineschi, V.

مصطلحات موضوعية: PMMRI, post-mortem imaging, sudden death

الوصف: During the last years, post-mortem imaging has gradually been assumed within research in the field of forensic pathology. This role appears to be clearly and simply applied in the trauma field with the use of conventional radiography or Post Mortem Computed Tomography (PMCT). Recently, particular attention was paid to cardiovascular imaging using Post Mortem Magnetic Resonance Imaging (PMMRI). The present experimental study aims to: (i) confirm the efficacy of a Post Mortem Cardiac Resonance Imaging (PMCRI) study protocol for the study of human hearts collected during the autopsy; (ii) apply the defined protocol on subjects who died of “SCD (sudden cardiac death)”, to identify alterations that could guide subsequent sampling. Two hearts of healthy subjects (A: male 22 years; B: female 26 years), who died from causes other than SCD were collected and compared to hearts that belonged to SCD individuals (C: male, 47 years old; D: female, 44 years old; E: male; 72 years old). The exams were performed on a 1.5 T scanner (Philips Intera Achieva, Best, the Netherlands) on hearts collected during autopsy and after a 30-day formalin fixation. Two readers analyzed the obtained images blindly and after randomization. From the comparison between the data from imaging and the macroscopic and histological investigations carried out, the present study proved the effectiveness of a PMMRI protocol to study ex-situ hearts. Moreover, it suggested the following semeiology in post-mortem SCD cases: the hyperintense area with indistinct margins in the Short Tau Inversion Recovery (STIR) sequence was linked to edema or area of pathological fibers, whereas the hypointense area in the T2-FFE sequences was linked to fibrosis. PMMRI can provide a valuable benefit to post-mortem investigations, helping to distinctly improve the success rate of histological sampling and investigations, which remains the gold standard in the diagnosis of sudden death.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/35054385; info:eu-repo/semantics/altIdentifier/wos/WOS:000747104700001; volume:12; issue:1; firstpage:1; lastpage:19; numberofpages:19; journal:DIAGNOSTICS; http://hdl.handle.net/11573/1649026Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85123076186

الإتاحة: https://doi.org/10.3390/diagnostics12010218Test
http://hdl.handle.net/11573/1649026Test

المؤلفون: Delogu, C, Fineschi, B, La Russa, R, Cipolloni, L, Morena, D, Frati, P

المساهمون: Delogu, C, Fineschi, B, La Russa, R, Cipolloni, L, Morena, D, Frati, P

مصطلحات موضوعية: Law n. 40/2004, MAP, Medically assisted procreation, embryo transfer, embryonal experimentation, informed consent

الوصف: Law n. 40/2004 regulated Medically Assisted Procreation (MAP) in Italy. The case described in this article is part of the many judicial cases that have affected this legislation. Following the marital separation process, the former husband withdrew consent to the implantation of already formed embryos in the uterus of his former wife who turned to the judiciary to obtain permission to initiate such a procedure. This paper consists of an analysis of this judicial process and deals with medical-legal and bioethical issues. In contrast to what is stated in Law n. 40/2004, the subsequent Law n. 219/2017 about informed consent, establishes the general principle of the withdrawal of consent until the healthcare procedure is carried out. Another issue concerns the difficult balance between the guarantee of personal freedom and the embryo's life protection, considering that although the embryo is already formed, it is not a person yet. In light of the existence of previous conflicting judicial sentences, the careful and separate assessment of the case under discussion, together with potential future events, is essential to reach a balanced conclusion, that can resolve disputes in the Italian constantly changing society.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/36373444; volume:173; issue:6; firstpage:507; lastpage:511; numberofpages:5; journal:LA CLINICA TERAPEUTICA; https://hdl.handle.net/11573/1677040Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85141697515

الإتاحة: https://doi.org/10.7417/CT.2022.2471Test
https://hdl.handle.net/11573/1677040Test

المؤلفون: Frisoni P., Diani L., De Simone S., Bosco M. A., Cipolloni L., Neri M.

المساهمون: Frisoni, P., Diani, L., De Simone, S., Bosco, M. A., Cipolloni, L., Neri, M.

مصطلحات موضوعية: aquaporin-5, drowning, freshwater, seawater, forensic investigation, immunohistochemistry

الوصف: Background and Objectives: Aquaporins are a family of water channel proteins. In this study, the renal and intrapulmonary expression of aquaporin-5 (AQP5) was examined in forensic autopsy cases to evaluate it as a drowning marker and to differentiate between freshwater drowning and saltwater drowning. Materials and Methods: Cases were classified into three groups: freshwater drowning (FWD), saltwater drowning (SWD), and controls (CTR). Samples were obtained from forensic autopsies at less than 72 h postmortem (15 FWD cases, 15 SWD cases, and 17 other cases) and were subjected to histological and immunohistochemical investigations. Results: In FWD group, intrapulmonary AQP5 expression was significantly suppressed compared with SWD and CTR; there was no significant difference in AQP5 expression among the other two groups. The same differences in expression were also observed in the kidney. Conclusions: These observations suggest that AQP5 expression in alveolar cells was suppressed by hypotonic water to prevent hemodilution. Moreover, it is possible to hypothesize that in the kidney, with the appearance of hypo-osmotic plasma, AQP5 is hypo-expressed, as a vital reaction, to regulate the renal reabsorption of water. In conclusion, the analysis of renal and intrapulmonary AQP5 expression would be forensically useful for differentiation between FWD and SWD, or between FWD and death due to other causes.

وصف الملف: STAMPA

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/36295616; info:eu-repo/semantics/altIdentifier/wos/WOS:000873088800001; volume:58; issue:10; firstpage:1458-1; lastpage:1458-8; numberofpages:8; journal:MEDICINA; https://hdl.handle.net/11392/2504650Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85140583192; https://www.mdpi.com/1648-9144/58/10/1458Test

الإتاحة: https://doi.org/10.3390/medicina58101458Test
https://hdl.handle.net/11392/2504650Test
https://www.mdpi.com/1648-9144/58/10/1458Test

المؤلفون: Bertozzi G., Ferrara M., La Russa R., Pollice G., Gurgoglione G., Frisoni P., Alfieri L., De Simone S., Neri M., Cipolloni L.

المساهمون: Bertozzi, G., Ferrara, M., La Russa, R., Pollice, G., Gurgoglione, G., Frisoni, P., Alfieri, L., De Simone, S., Neri, M., Cipolloni, L.

مصطلحات موضوعية: decomposed body, GPA, IHC, MMP-9, wound vitality

الوصف: Background: The question about wound vitality and the estimation of wound age of production are two of the classic investigation fields of forensic sciences. To answer this, the techniques most frequently used in research studies are immunohistochemistry (IHC), molecular biology, and biochemistry. Despite the great data on the literature about the usefulness of IHC in forensic pathology, there is always a request for further studies, especially on tissues altered by putrefactive phenomena. In fact, the degradation of the tissues is intended as the main limiting factor to the use of this technique. Scope: The aim of this pilot study was to evaluate the immunohistochemical behavior of samples collected from decomposed bodies (in different putrefaction phases) and to relate these findings to wound vitality and postmortem interval. Materials and Methods: Samples of skin and soft tissues were collected during autopsies, which were executed on decomposed bodies, whose cause of death was concluded to be traumatic. An immunohistochemical study was performed using antibodies against CD15, CD45, IL-15, tryptase, and glycophorin-A MMPs (endopeptidases involved in degrading extracellular matrix proteins: MMP-9 and MMP-2). An immunohistochemistry (IHC) reaction was evaluated according to a qualitative method as the following legend: (0): not expressed, (+): isolated and disseminated expression, (++): expression in groups or widespread foci, and (+++): widespread expression. Results: Most of the tested markers (tryptase, glycophorin, IL15, CD 15, CD 45, and MMP9) showed to be highly expressed in the tissue of putrefied skin for 15 days. Discussion and Conclusion: Although certainly inconclusive, this experimental application demonstrated that a nonexclusive but combined use of multiple antibodies is appropriate to verify wound vitality in decomposed bodies. Among them, GPA exhibited major reliability.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/35004780; info:eu-repo/semantics/altIdentifier/wos/WOS:000743298800001; volume:8; firstpage:1; lastpage:7; numberofpages:7; journal:FRONTIERS IN MEDICINE; http://hdl.handle.net/11573/1649028Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85122445067

الإتاحة: https://doi.org/10.3389/fmed.2021.802841Test
http://hdl.handle.net/11573/1649028Test

المؤلفون: Baldari B., Vittorio S., Sessa F., Cipolloni L., Bertozzi G., Neri M., Cantatore S., Fineschi V., Aromatario M.

المساهمون: Baldari, B., Vittorio, S., Sessa, F., Cipolloni, L., Bertozzi, G., Neri, M., Cantatore, S., Fineschi, V., Aromatario, M.

مصطلحات موضوعية: forensic pathology, glycophorin A investigation, vitality

الوصف: Glycophorins are an important group of red blood cell (RBC) transmembrane proteins. Monoclonal antibodies against GPA are employed in immunohistochemical staining during postmortem examination: Through this method, it is possible to point out the RBC presence in tissues. This experimental study aims to investigate anti-GPA immunohistochemical staining in order to evaluate the vitality of the lesion from corpses in different decomposition state. Six cases were selected, analyzing autopsies’ documentation performed by the Institute of Legal Medicine of Rome in 2010-2018: four samples of fractured bones and three samples of soft tissues. For the control case, the fracture region of the femur was sampled. The results of the present study confirm the preliminary results of other studies, remarking the importance of the GPA immunohistochemical staining to highlight signs of survival. Moreover, this study suggests that the use of this technique should be routinely applied in cases of corpses with advanced putrefaction phenomena, even when the radiological investigation is performed, the macroscopic investigation is inconclusive, the H&E staining is not reliable. This experimental application demonstrated that the use of monoclonal antibody anti-human GPA on bone fractures and soft tissues could be important to verify whether the lesion is vital or not.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/33946627; info:eu-repo/semantics/altIdentifier/wos/WOS:000653942700001; volume:9; issue:5; firstpage:1; lastpage:10; numberofpages:10; journal:HEALTHCARE; http://hdl.handle.net/11573/1575469Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85105597544

الإتاحة: https://doi.org/10.3390/healthcare9050514Test
http://hdl.handle.net/11573/1575469Test

المؤلفون: Bertozzi G., Maiese A., Passaro G., Tosoni A., Mirijello A., De Simone S., Baldari B., Cipolloni L., La Russa R.

المساهمون: Bertozzi, G., Maiese, A., Passaro, G., Tosoni, A., Mirijello, A., De Simone, S., Baldari, B., Cipolloni, L., La Russa, R.

مصطلحات موضوعية: Chemotherapy-induced damage, Neutropenic enterocoliti, Sepsi, Adult, Child, Human, Prognosi, Enterocolitis, Neutropenic, Lymphoma, Neoplasm, Sepsis

الوصف: Background: Neutropenic enterocolitis (NE), which in the past was also known as typhlitis or ileocecal syndrome for the segment of the gastrointestinal tract most affected, is a nosological entity that is difficult to diagnose and whose pathogenesis is not fully known to date. Initially described in pediatric patients with leukemic diseases, it has been gradually reported in adults with hematological malignancies and non-hematological conditions, such as leukemia, lymphoma, multiple myeloma, aplastic anemia, and also myelodysplastic syndromes, as well as being associated with other immunosuppressive causes such as AIDS treatment, therapy for solid tumors, and organ transplantation. Therefore, it is associated with high mortality due to the rapid evolution in worse clinical pictures: Rapid progression to ischemia, necrosis, hemorrhage, perforation, multisystem organ failure, and sepsis. Case report: A case report is included to exemplify the clinical profile of patients with NE who develop sepsis. Literature Review: To identify a specific profile of subjects affected by neutropenic enterocolitis and the entity of the clinical condition most frequently associated with septic evolution, a systematic review of the literature was conducted. The inclusion criteria were as follows: English language, full-text availability, human subjects, and adult subjects. Finally, the papers were selected after the evaluation of the title and abstract to evaluate their congruity with the subject of this manuscript. Following these procedures, 19 eligible empirical studies were included in the present review. Conclusions: Despite the recent interest and the growing number of publications targeting sepsis and intending to identify biomarkers useful for its diagnosis, prognosis, and for the understanding of its pathogenesis, and especially for multi-organ dysfunction, and despite the extensive research period of the literature review, the number of publications on the topic “neutropenic enterocolitis and sepsis” appears to be very ...

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/34203105; info:eu-repo/semantics/altIdentifier/wos/WOS:000666806400001; volume:57; issue:6; numberofpages:10; journal:MEDICINA; http://hdl.handle.net/11568/1121558Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85109134910; https://www.mdpi.com/1648-9144/57/6/638Test

الإتاحة: https://doi.org/10.3390/medicina57060638Test
http://hdl.handle.net/11568/1121558Test
https://www.mdpi.com/1648-9144/57/6/638Test

المؤلفون: Aromatario M., Torsello A., D'errico S., Bertozzi G., Sessa F., Cipolloni L., Baldari B.

المساهمون: Aromatario, M., Torsello, A., D'Errico, S., Bertozzi, G., Sessa, F., Cipolloni, L., Baldari, B.

مصطلحات موضوعية: epidural hematoma, forensic histopathology, interdural hematoma, subdural hematoma, adult, hematoma, subdural, human, retrospective studie, brain injuries, traumatic, epidural, cranial, suicide

الوصف: Epidural hematomas (EDHs) and subdural hematomas (SDHs), or so-called extra-axial bleedings, are common clinical entities after a traumatic brain injury (TBI). A forensic pathologist often analyzes cases of traumatic EDHs or SDHs due to road accidents, suicides, homicides, assaults, domestic or on-the-job accidents, and even in a medical responsibility scenario. The aim of this review is to give an overview of the published data in the medical literature, useful to forensic pathologists. We mainly focused on the data from the last 15 years, and considered the most updated protocols and diagnostic-therapeutic tools. This study reviews the epidemiology, outcome, and dating of extra-axial hematomas in the adult population; studies on the controversial interdural hematoma are also included.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/33535407; info:eu-repo/semantics/altIdentifier/wos/WOS:000622725000001; volume:57; issue:2; firstpage:1; lastpage:16; numberofpages:16; journal:MEDICINA; http://hdl.handle.net/11573/1558571Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85100548256

الإتاحة: https://doi.org/10.3390/medicina57020125Test
http://hdl.handle.net/11573/1558571Test

المؤلفون: Fineschi V., Aprile A., Aquila I., Arcangeli M., Asmundo A., Bacci M., Cingolani M., Cipolloni L., D'Errico S., de Casamassimi I., Mizio G. D., Paolo M. D., Focardi M., Frati P., Gabbrielli M., Russa R. L., Maiese A., Manetti F., Martelloni M., Mazzeo E., Montana A., Neri M., Padovano M., Pinchi V., Pomara C., Ricci P., Salerno M., Santurro A., Scopetti M., Testi R., Turillazzi E., Vacchiano G., Crivelli F., Bonoldi E., Facchetti F., Nebuloni M., Sapino A.

المساهمون: Fineschi, V., Aprile, A., Aquila, I., Arcangeli, M., Asmundo, A., Bacci, M., Cingolani, M., Cipolloni, L., D'Errico, S., de Casamassimi, I., Mizio, G. D., Paolo, M. D., Focardi, M., Frati, P., Gabbrielli, M., Russa, R. L., Maiese, A., Manetti, F., Martelloni, M., Mazzeo, E., Montana, A., Neri, M., Padovano, M., Pinchi, V., Pomara, C., Ricci, P., Salerno, M., Santurro, A., Scopetti, M., Testi, R., Turillazzi, E., Vacchiano, G., Crivelli, F., Bonoldi, E., Facchetti, F., Nebuloni, M., Sapino, A.

مصطلحات موضوعية: Autopsy, Body Fluids, COVID-19, Cadaver, Coronavirus Infections, Health Personnel, Humans, Italy, Morgue, Pandemics, Personal Protective Equipment

الوصف: The COVID-19 (Coronavirus Disease-19) is the most urgent health emergency worldwide and all professionals are called to give support in the diagnosis and treatment of patients affected by this disease. The Scientific Society of Hospital Legal Medicine of the National Health System (COMLAS) and the Italian Society of Anatomical Pathology and Cytology (SIAPEC) produced this document with the intent of offering a technical support to professional involved in the autoptic activities during the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) epidemic infection.

وصف الملف: ELETTRONICO

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/32324727; info:eu-repo/semantics/altIdentifier/wos/WOS:000605747900004; volume:112; issue:2; firstpage:64; lastpage:77; numberofpages:14; journal:PATHOLOGICA; http://hdl.handle.net/11392/2433399Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85083062580; https://www.pathologica.it/article/view/101Test

الإتاحة: https://doi.org/10.32074/1591-951X-13-20Test
http://hdl.handle.net/11392/2433399Test
https://www.pathologica.it/article/view/101Test