المؤلفون: Julien Herman, Benoit Le Goff, Julien De Lima, Régis Brion, Catherine Chevalier, Frédéric Blanchard, Christelle Darrieutort-Laffite

المصدر: Arthritis Research & Therapy, Vol 23, Iss 1, Pp 1-11 (2021)

مصطلحات موضوعية: Apatite, Rotator cuff tendons, Interleukin-1, Inflammasome, Air pouch model, Diseases of the musculoskeletal system, RC925-935

الوصف: Abstract Background Calcific tendonitis of the rotator cuff is due to carbonated apatite deposits in the shoulder tendons. During the evolution of the disease, an acute inflammatory episode may occur leading to the disappearance of the calcification. Although hydroxyapatite crystal-induced inflammation has been previously studied with synthetic crystals, no data are available with calcifications extracted from patients suffering from calcific tendinopathy. The objective of the study was to explore the inflammatory properties of human calcifications and the pathways involved. Methods Human calcifications and synthetic hydroxyapatite were used in vitro to stimulate human monocytes and macrophages, the human myeloid cell line THP-1, and human tenocytes. The release of IL-1β, IL-6, and IL-8 by cells was quantified by ELISA. The gene expression of pro- and anti-inflammatory cytokines was evaluated by quantitative PCR. NF-kB activation and NLRP3 involvement were assessed in THP-1 cells using a NF-kB inhibitor and a caspase-1 inhibitor. The inflammatory properties were then assessed in vivo using a mouse air pouch model. Results Human calcifications were able to induce a significant release of IL-1β when incubated with monocytes, macrophages, and THP-1 only if they were first primed with LPS (monocytes and macrophages) or PMA (THP-1). Stimulation of THP-1 by human calcifications led to similar levels of IL-1β when compared to synthetic hydroxyapatite although these levels were significantly inferior in monocytes and macrophages. The patient’s crystals enhanced mRNA expression of pro-IL-1β, as well as IL-18, NF-kB, and TGFβ when IL-6 and TNFα expression were not. IL-1β production was reduced by the inhibition of caspase-1 indicating the role of NLRP3 inflammasome. In vivo, injection of human calcifications or synthetic hydroxyapatite in the air pouch led to a significant increase in membrane thickness although significant overexpression of IL-1β was only observed for synthetic hydroxyapatite. Conclusions As synthetic hydroxyapatite, human calcifications were able to induce an inflammatory response resulting in the production of IL-1β after NF-kB activation and through NLRP3 inflammasome. In some experiments, IL-1β induction was lower with human calcifications compared to synthetic apatite. Differences in size, shape, and protein content may explain this observation.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1478-6362Test

الوصول الحر: https://doaj.org/article/b19c7c275c67410ba2bb12dee497d5c3Test

المؤلفون: Christelle Darrieutort-Laffite, Catherine Ansquer, Hélène Aubert, Françoise Kraeber-Bodéré, Agathe Masseau, Christian Agard, Mohamed Hamidou, Claire Bernier, Jean-Marie Berthelot, Benoit Le Goff, Sébastien Barbarot, Antoine Néel

المصدر: Arthritis Research & Therapy, Vol 22, Iss 1, Pp 1-10 (2020)

مصطلحات موضوعية: Bone scan, Bone lesions, Interleukin 1 receptor antagonist, Schnitzler syndrome, Diseases of the musculoskeletal system, RC925-935

الوصف: Abstract Objective To report on the characteristics and long-term course of rheumatic manifestations in Schnitzler syndrome (SchS). Methods A retrospective cohort study of patients with SchS followed between 2000 and 2020. Inclusion criteria included a diagnosis of SchS (Strasbourg criteria). All available bone scans were reviewed and scored according to the intensity and number of pathological sites. The scintigraphic score was compared with the clinical activity score, CRP level, and treatments. Results Twenty-five patients were included. Median age at diagnosis was 68 years. Eighty patients (72%) had SchS-related rheumatic pain. Most patients had a long-standing isolated rash before constitutional and/or rheumatic symptoms appeared. The monoclonal component level was usually very low (IgMκ in 22/25). Rheumatic pain predominated around the knees. Bone scans revealed abnormal tracer uptake in 15/18 (85%). The scintigraphic score correlated with clinical activity (r = 0.4, p

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s13075-020-02318-5Test; https://doaj.org/toc/1478-6362Test

الوصول الحر: https://doaj.org/article/fabbff27aafb4999bfe695d9fd6de2a6Test

المؤلفون: Yves Maugars, Pascale Guillot, Joëlle Glémarec, Jean-Marie Berthelot, Benoit Le Goff, Christelle Darrieutort-Laffite

المصدر: Journal of Medical Case Reports, Vol 14, Iss 1, Pp 1-7 (2020)

مصطلحات موضوعية: Osteoporosis, Denosumab rebound, Fracture, Hypercalcemia, Hyperparathyroidism, Medicine

الوصف: Abstract Background The rebound effect after stopping treatment with denosumab may be associated with rapid loss of the gains in bone mineral density achieved with treatment, high levels of bone remodeling markers, the occurrence of vertebral fractures, and even hypercalcemia. Case presentation A 64-year-old osteoporotic Caucasian woman suffered from a fracture of her second lumbar vertebra in 2004. From January 2005, she was treated with denosumab for 9 years, with good densitometry results for her hip and lumbar areas, and no fractures over the last 6 years of treatment. Ten months after the treatment with denosumab was stopped, a cascade of vertebral fractures, including some in unusual locations (third thoracic vertebra), and multiple rib fractures in a context of hypercalcemia, suggested possible malignancy. A complete evaluation, including systemic, biological, and biopsy analyses, ruled out this hypothesis. The hypercalcemia was associated with normal plasma phosphate and vitamin D concentrations, and a high parathyroid hormone level, with an abnormal fixation of the lower lobe of the thyroid on sesta-methoxy-isobutyl-isonitrile scintigraphy. Histological analysis of the excised parathyroid tissue revealed hyperplasia. The associated thyroidectomy (goiter) led to the discovery of a thyroid papillary microcarcinoma. Conclusions We consider the consequences of this rebound effect, not only in terms of the major loss of bone density (return to basal values within 3 years) and the multiple disabling fracture episodes, but also in terms of the hypercalcemia observed in association with apparently autonomous tertiary hyperparathyroidism. Several cases of spontaneous reversion have been reported in children, but the intervention in our patient precluded any assessment of the possible natural course. The discovery of an associated thyroid neoplasm appears to be fortuitous. Better understanding of the various presentations of the rebound effect after stopping treatment with denosumab would improve diagnostic management of misleading forms, as in this case. Bisphosphonates could partially prevent this rebound effect.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s13256-020-02401-0Test; https://doaj.org/toc/1752-1947Test

الوصول الحر: https://doaj.org/article/2aa7eb9965a0448c98dcb1d8a7eb6751Test

المؤلفون: Yves Maugars, Jean-Marie Berthelot, Benoit Le Goff, Christelle Darrieutort-Laffite

المصدر: Frontiers in Medicine, Vol 8 (2021)

مصطلحات موضوعية: fibromyalgia, pain, fatigue, diagnosis, psyche, neuro-imaging, Medicine (General), R5-920

الوصف: The concept of fibromyalgia has progressed to achieve a certain consensus regarding the definition of the condition. We summarize what is known in 2020, be it in terms of diagnosis, with the criteria that have changed over the years, or at the level of the psychological profile, via the notions of “catastrophizing” and “coping” and post-traumatic syndrome. The importance of fatigue and sleep disorders is underlined, with the chronological sequence of post-traumatic syndrome, chronic fatigue, and then amplification of the pain and the onset of multiple associated symptoms. The etiopathogenic debate has been enriched thanks to neuro-imaging data to discover the start of the central neurological signature. The many associated symptoms are reanalyzed in the context of so-called sister conditions which form sometimes more or less separate entities, such as chronic fatigue syndrome or restless legs syndrome for example. What these conditions have in common is hypersensitivity, not just to pain, but also to all exteroceptive stimuli, from deep sensitivity in the neuro-vegetative system, the sense organs and certain functions of the central nervous system, to the psychological aspects and sleep control. In summary, it is possible to define fibromyalgia as a cognitive disorder of cortical integration of chronic pain, with amplification of painful and sensory nociception, decrease in the threshold for the perception of pain, and persistence of a stimulus that maintains the process in chronicity. Fibromyalgia is part of a group of chronic hypersensitivity syndromes of central origin, with a very wide range of means of expression.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fmed.2021.666914/fullTest; https://doaj.org/toc/2296-858XTest

الوصول الحر: https://doaj.org/article/d6872a6fc67c4a24b92c5823ecbd5825Test

المؤلفون: Jean-Marie Berthelot, Noura Biha, Christelle Darrieutort-Laffite, Benoît Le Goff, Yves Maugars

المصدر: PAIN Reports, Vol 4, Iss 3, p e739 (2019)

مصطلحات موضوعية: Anesthesiology, RD78.3-87.3

الوصف: Abstract. Objectives:. We aimed to compare painDETECT scores in outpatients seen in a rheumatology department over a 1-month period and search for correlations between painDETECT scores and the estimated duration of daily pain and time elapsed since the onset of current pain. Patients and Methods:. A total of 529 of 738 outpatients agreed to complete a set of questionnaires, including painDETECT. Results:. The mean painDETECT score was 14.14 ± 7.59, and 31% of the patients had painDETECT scores of >18. Fibromyalgia ranked first (21.2 ± 6.0), followed by osteoarthritis of the lower limbs (17.8 ± 8.2), back pain and radiculopathies (16.1 ± 6.8), osteoarthritis of the upper limbs (15.7 ± 8.1), spondylarthrosis (15.1 ± 7.2), entrapment neuropathies (14.1 ± 2.4), rheumatoid arthritis (13.8 ± 7.1), miscellaneous conditions (13.8 ± 8.2), tendinitis (13.4 ± 7.9), connectivitis (11.5 ± 6.7), and osteoporosis (8.5 ± 6.9). The duration of daily pain was much longer in patients with painDETECT scores of >18 (12.41 ± 8.45 vs 6.53 ± 7.45 hours) (t = 0.0000), but very similar painDETECT scores were observed for patients suffering from pain for less than 1 week (13.7 ± 8.2; 38% > 18), for 1 month (14.5 ± 8.2; 25% > 18), several months (12.7 ± 7.3; 23% > 18), 1 year (13.8 ± 7.7; 29% > 18), or several years (14.7 ± 7.4; 33% > 18). Conclusion:. PainDETECT scores differed little depending on the musculoskeletal condition, strongly correlated with the duration of daily pain, and appeared to be as high in patients with recent pain as in those suffering for years.

وصف الملف: electronic resource

العلاقة: http://journals.lww.com/painrpts/fulltext/10.1097/PR9.0000000000000739Test; https://doaj.org/toc/2471-2531Test

الوصول الحر: https://doaj.org/article/c38f5f2ce33440f99aa5c6c3608c6111Test

المؤلفون: Christelle Darrieutort-Laffite, Louis J. Soslowsky, Benoit Le Goff

المصدر: International Journal of Molecular Sciences, Vol 21, Iss 19, p 7000 (2020)

مصطلحات موضوعية: Achilles tendinopathy, ultrasound-guided injections, platelet-rich plasma, stem cells, dry needling, Biology (General), QH301-705.5, Chemistry, QD1-999

الوصف: Achilles tendinopathy (AT) is a common problem, especially in people of working age, as well as in the elderly. Although the pathogenesis of tendinopathy is better known, therapeutic management of AT remains challenging. Various percutaneous treatments have been applied to tendon lesions: e.g., injectable treatments, platelet-rich plasma (PRP), corticosteroids, stem cells, MMP inhibitors, and anti-angiogenic agents), as well as percutaneous procedures without any injection (percutaneous soft tissue release and dry needling). In this review, we will describe and comment on data about the molecular and structural effects of these treatments obtained in vitro and in vivo and report their efficacy in clinical trials. Local treatments have some impact on neovascularization, inflammation or tissue remodeling in animal models, but evidence from clinical trials remains too weak to establish an accurate management plan, and further studies will be necessary to evaluate their value.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1422-0067/21/19/7000Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test

الوصول الحر: https://doaj.org/article/9751dbc1fa734c0ebd72bb9ee0362936Test

المؤلفون: Christelle Darrieutort-Laffite, Paul Arnolfo, Thomas Garraud, Annie Adrait, Yohann Couté, Guy Louarn, Valérie Trichet, Pierre Layrolle, Benoit Le Goff, Frédéric Blanchard

المصدر: Journal of Clinical Medicine, Vol 8, Iss 10, p 1544 (2019)

مصطلحات موضوعية: calcific tendonitis, rotator cuff, apatite, hypertrophic chondrocytes, tissue non-specific alkaline phosphatase, Medicine

الوصف: Calcific tendonitis is a frequent cause of chronic shoulder pain. Its cause is currently poorly known. The objectives of this study were to better characterize the cells and mechanisms involved in depositing apatite crystals in human tendons. Histologic sections of cadaveric calcified tendons were analyzed, and human calcific deposits from patients undergoing lavage of their calcification were obtained to perform infrared spectroscopy and mass spectrometry-based proteomic characterizations. In vitro, the mineralization ability of human rotator cuff cells from osteoarthritis donors was assessed by alizarin red or Von Kossa staining. Calcifications were amorphous areas surrounded by a fibrocartilaginous metaplasia containing hypertrophic chondrocyte-like cells that expressed tissue non-specific alkaline phosphatase (TNAP) and ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), which are two key enzymes of the mineralization process. Calcific deposits were composed of apatite crystals associated with proteins involved in bone and cartilage development and endochondral bone growth. In vitro, tenocyte-like cells extracted from the rotator cuff were able to mineralize in osteogenic cultures, and expressed TNAP, type X COLLAGEN, and MMP13, which are hypertrophic chondrocytes markers. The use of a TNAP inhibitor significantly prevented mineral deposits. We provide evidence that tenocytes have a propensity to differentiate into hypertrophic chondrocyte-like cells to produce TNAP-dependent calcium deposits. We believe that these results may pave the way to identifying regulating factors that might represent valuable targets in calcific tendonitis.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2077-0383/8/10/1544Test; https://doaj.org/toc/2077-0383Test

الوصول الحر: https://doaj.org/article/4a09b66646784ec894c792bfb5b3e3d4Test

المؤلفون: Christelle Darrieutort-Laffite, Marie-Astrid Boutet, Mathias Chatelais, Régis Brion, Frédéric Blanchard, Dominique Heymann, Benoit Le Goff

المصدر: Mediators of Inflammation, Vol 2014 (2014)

مصطلحات موضوعية: Pathology, RB1-214

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/0962-9351Test; https://doaj.org/toc/1466-1861Test

الوصول الحر: https://doaj.org/article/9e36fab9d15c42f3bc21b2580a2d08aeTest

المؤلفون: Julien Herman (10727197), Benoit Le Goff (9658398), Julien De Lima (8640990), Régis Brion (530868), Catherine Chevalier (197486), Frédéric Blanchard (9511728), Christelle Darrieutort-Laffite (9658389)

مصطلحات موضوعية: Biochemistry, Medicine, Microbiology, Cell Biology, Molecular Biology, Physiology, Immunology, Developmental Biology, Cancer, Infectious Diseases, Space Science, Chemical Sciences not elsewhere classified, Apatite, Rotator cuff tendons, Interleukin-1, Inflammasome, Air pouch model

الوصف: Additional file 1: Supplementary Figure S1. IL-1β release after 6h stimulation of CD14+ monocytes, MCSF-Macrophages, GM-CSF/IFNγ-Macrophages by crystals at 250, 500 and 1000 ng/ml in presence of LPS (lipopolysaccharide) and parallel cell death rate. sHA= synthetic hydroxyapatite, CAL = human calcification CT= control group without crystal; LPS= lipopolysaccharide. n = 3. * p≤ 0,05. Supplementary Figure S2. Western Blot raw images of sHA (positive control) and three patients. Cleaved IL-1β was studied in cellular lysates (CL) and in supernatants (SN). iNF-κB= NF-κB inhibitor; iCasp1= caspase-1 inhibitor; inh= inhibitor; sHA= synthetic hydroxyapatite; CAL= human calcification; CT = control condition without crystals. Supplementary Table 1. Primers used for quantitative Polymerase Chain Reaction.

العلاقة: https://figshare.com/articles/journal_contribution/Additional_file_1_of_Pro-inflammatory_effects_of_human_apatite_crystals_extracted_from_patients_suffering_from_calcific_tendinopathy/14516698Test

الإتاحة: https://doi.org/10.6084/m9.figshare.14516698.v1Test

المؤلفون: Jean-Marie Berthelot, Christelle Darrieutort-Laffite, Caroline Trang, Yves Maugars, Benoît Le Goff

المصدر: Revue du Rhumatisme. 89:359-364

مصطلحات موضوعية: Rheumatology

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::e15856339c877000ec3f5a23e36640b4Test
https://doi.org/10.1016/j.rhum.2022.03.007Test