المؤلفون: Benoit Le Goff, Jean-Marie Berthelot, Christelle Darrieutort-Laffite

المصدر: Joint Bone Spine. 88:105142

مصطلحات موضوعية: education.field_of_study, medicine.medical_specialty, business.industry, Population, Cohort Studies, Clinical trial, Rheumatology, Surveys and Questionnaires, Family medicine, Cohort, Humans, Medicine, education, business, Follow-Up Studies

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eff28ebe17292ceff4a7d3696c82bb69Test
https://doi.org/10.1016/j.jbspin.2021.105142Test

المؤلفون: B. Le Goff, Christelle Darrieutort-Laffite, J. De Lima, Régis Brion, Catherine Chevalier, J B Herman, Frédéric Blanchard

المصدر: Annals of the Rheumatic Diseases. 80:413.1-413

مصطلحات موضوعية: Pathology, medicine.medical_specialty, Rheumatology, Apatite crystals, business.industry, Immunology, Immunology and Allergy, Medicine, Calcific tendinopathy, business, General Biochemistry, Genetics and Molecular Biology

الوصف: Background:Calcific tendonitis of the rotator cuff is due to carbonated apatite deposits in the shoulder tendons. During the evolution of the disease, an acute inflammatory episode may occur leading to the disappearance of the calcification. Although hydroxyapatite crystals-induced inflammation has been previously studied with synthetic crystals, no data are available with calcifications extracted from patients suffering from calcific tendinopathy. The objective of the study was to explore the inflammatory properties of human calcifications and the pathways involved.Objectives:The objective of the study was to explore the inflammatory properties of human calcifications and the pathways involved.Methods:Human calcifications were obtained from patients treated for their shoulder pain related to a calcific tendinopathy of the rotator cuff. Calcifications were extracted by ultrasound-guided lavage and aspiration as previously described [1]. Human calcifications and synthetic hydroxyapatite (sHA) were used in vitro to stimulate human monocytes and macrophages, the human myeloid cell line THP-1 and human tenocytes. The release of IL-1β, IL-6 and IL-8 by cells was quantified by ELISA. Gene expression of pro- and anti-inflammatory cytokines was evaluated by quantitative PCR. NF-kB activation and NLRP3 involvement was assessed in THP-1 cells using a NF-kB inhibitor and a Caspase 1 inhibitor. The inflammatory properties were then assessed in vivo using a mouse air pouch model. The membrane thickness and infiltrate were assessed 6 and 24 hours after the injection of human calcifications or synthetic hydroxyapatite using hematoxylin and eosin staining. Macrophages, neutrophils and lymphocytes infiltrates were assessed by immunohistochemistry. Total RNA was extracted from the membranes and expression of IL-1β, IL-6 and TNFβ was quantified by PCR.Results:Human calcifications were able to induce a significant release of IL-1β when incubated with monocytes, macrophages and THP-1 only if they were first primed with LPS (lipopolysaccharide) for monocytes and macrophages or PMA (Phorbol 12-myristate 13-acetate) for THP-1. No IL-1β was detected in tenocytes’ supernatants. Stimulation of THP-1 by human calcifications led to similar levels of IL-1β when compared to synthetic hydroxyapatite although these levels were significantly inferior in monocytes and macrophages. IL-6 and IL-8 levels were not increased in the supernatants after crystal stimulation. Patient’s crystals enhanced mRNA expression of pro-IL-1β, as well as IL-18, NF-kB and TGFβ when IL-6 and TNFα expression were not. IL-1β production was reduced by the inhibition NF-kB as well as Caspase 1 indicating the role of NLRP3 inflammasome. In vivo, injection of human calcifications or synthetic hydroxyapatite in air pouch led to significant increase in membrane thickness with an infiltrate mainly composed of macrophages. Significant overexpression of IL-1β was only observed in the synthetic hydroxyapatite group.Conclusion:As synthetic hydroxyapatite, human calcifications were able to induce an inflammatory response resulting in the production of IL-1β after NF-kB activation and through NLRP3 inflammasome. In some experiments, IL-1β induction was lower with human calcifications compared to synthetic apatite. Differences in size, shape and protein content may explain this observation.References:[1]Darrieutort-Laffite C, Arnolfo P, Garraud T, Adrait A, Couté Y, Louarn G, et al. Rotator Cuff Tenocytes Differentiate into Hypertrophic Chondrocyte-Like Cells to Produce Calcium Deposits in an Alkaline Phosphatase-Dependent Manner. J Clin Med. 2019 Sep 26;8(10):1544. doi: 10.3390/jcm8101544.Acknowledgements:Fondation Arthritis, Recherche et Rhumatismes and French Society for Rheumatology for their financial supportDisclosure of Interests:None declared

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::5443020e047ba8052350547fb3fa2cb6Test
https://doi.org/10.1136/annrheumdis-2021-eular.2624Test

المؤلفون: Géraldine Bart, Lucie Planche, Yves Maugars, Joëlle Glémarec, Benoit Le Goff, Christelle Darrieutort-Laffite

المصدر: Revue du Rhumatisme. 84:61-67

مصطلحات موضوعية: 030203 arthritis & rheumatology, 03 medical and health sciences, 0302 clinical medicine, Rheumatology, 030202 anesthesiology

الوصف: Resume L’echographie est couramment utilisee en rhumatologie pour etudier et guider les infiltrations des articulations peripheriques. Cette technique peut egalement fournir des informations utiles sur l’anatomie du rachis lombaire. Des etudes ont montre que l’examen echographique du rachis etait un outil efficace pour faciliter la realisation de l’anesthesie epidurale. L’objectif de cette etude etait de determiner si le choix du niveau optimal d’infiltration par echographie pouvait faciliter l’injection epidurale de corticoides dans les cas ou l’infiltration etait presumee difficile (IMC > 30 kg/m2, âge > 60 ans ou scoliose lombaire). Methodes Nous avons realise un essai comparatif randomise. Quatre-vingts patients ont ete randomises dans deux groupes : le groupe echographie (n = 40) qui a beneficie d’une echographie rachidienne prealable pour determiner le niveau lombaire optimal pour l’injection, ou le groupe de temoin (n = 40) chez qui le niveau d’injection a ete determine par palpation. Le critere d’evaluation principal etait la douleur ressentie durant la procedure, evaluee par l’echelle visuelle analogique (EVA). Resultats Nous avons constate une correlation positive entre la profondeur de l’espace epidural et l’IMC (p Conclusion L’echographie du rachis lombaire a pu etre realisee chez les patients presentant une affection lombaire, meme obeses et âges, et a permis de visualiser l’espace epidural. Toutefois, l’examen echographique prealable n’a pas permis d’attenuer la douleur lors de la procedure.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::f62b65bff239107bf2bdc497d0307d40Test
https://doi.org/10.1016/j.rhum.2016.11.006Test

المؤلفون: Benoit Le Goff, Jean-Marie Berthelot, Noura Biha, Yves Maugars, Christelle Darrieutort-Laffite

المصدر: Pain Reports
PAIN Reports, Vol 4, Iss 3, p e739 (2019)

مصطلحات موضوعية: Osteoporosis, Tendinopathies, 02 engineering and technology, Osteoarthritis, Daily, 01 natural sciences, lcsh:RD78.3-87.3, Tendinitis, Fibromyalgia, 0103 physical sciences, 0202 electrical engineering, electronic engineering, information engineering, Back pain, Medicine, 010306 general physics, Back, business.industry, Arthritis, Spondylarthrosis, medicine.disease, Anesthesiology and Pain Medicine, Duration, lcsh:Anesthesiology, Rheumatoid arthritis, Anesthesia, Musculoskeletal, PainDetect, 020201 artificial intelligence & image processing, medicine.symptom, Neuropathic, business, Radiculopathies, Research Paper

الوصف: Objectives:. We aimed to compare painDETECT scores in outpatients seen in a rheumatology department over a 1-month period and search for correlations between painDETECT scores and the estimated duration of daily pain and time elapsed since the onset of current pain. Patients and Methods:. A total of 529 of 738 outpatients agreed to complete a set of questionnaires, including painDETECT. Results:. The mean painDETECT score was 14.14 ± 7.59, and 31% of the patients had painDETECT scores of >18. Fibromyalgia ranked first (21.2 ± 6.0), followed by osteoarthritis of the lower limbs (17.8 ± 8.2), back pain and radiculopathies (16.1 ± 6.8), osteoarthritis of the upper limbs (15.7 ± 8.1), spondylarthrosis (15.1 ± 7.2), entrapment neuropathies (14.1 ± 2.4), rheumatoid arthritis (13.8 ± 7.1), miscellaneous conditions (13.8 ± 8.2), tendinitis (13.4 ± 7.9), connectivitis (11.5 ± 6.7), and osteoporosis (8.5 ± 6.9). The duration of daily pain was much longer in patients with painDETECT scores of >18 (12.41 ± 8.45 vs 6.53 ± 7.45 hours) (t = 0.0000), but very similar painDETECT scores were observed for patients suffering from pain for less than 1 week (13.7 ± 8.2; 38% > 18), for 1 month (14.5 ± 8.2; 25% > 18), several months (12.7 ± 7.3; 23% > 18), 1 year (13.8 ± 7.7; 29% > 18), or several years (14.7 ± 7.4; 33% > 18). Conclusion:. PainDETECT scores differed little depending on the musculoskeletal condition, strongly correlated with the duration of daily pain, and appeared to be as high in patients with recent pain as in those suffering for years.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26400756998df32727fc18cf0b18d300Test
http://europepmc.org/articles/PMC6749904Test

المؤلفون: Frédéric Blanchard, B. Le Goff, Aurélie Najm, Paul Arnolfo, T Garraud, Christelle Darrieutort-Laffite

المصدر: Abstracts.

مصطلحات موضوعية: business.industry, Scleraxis, Phosphodiesterase, chemistry.chemical_element, Calcium, Phenotype, Tendon, Tenomodulin, Staining, Andrology, medicine.anatomical_structure, chemistry, medicine, Rotator cuff, business

الوصف: Career situation of first and presenting author Resident. Introduction Calcific tendinopathy represents 10% to 42% of chronic painful shoulders. These calcium deposits are composed of carbonated apatite. Although the disease is frequent, its origin stays still largely unknown. Our previous results showed that calcific deposits are surrounded by chondrocyte-like cells expressing TNAP (Tissue Nonspecific Alkaline Phosphatase) and ENPP1 (Ectonucleotidepyrophosphatase/phosphodiesterase 1), two key enzymes involved in the mineralization process. Objectives To study the ability of cells extracted from rotator cuff tendons to produce apatite crystals and to analyze the phenotype of these mineralizing cells. Methods Tenocytes were extracted from rotator cuff tendons removed during shoulder total replacement. To evaluate their ability to mineralize, they were cultured in an osteogenic medium (OM) for 21 days. Mineral deposition then was assessed by staining with Alizarin red. Tenocytes total RNA was extracted and analyzed by RT-qPCRs. TNAP enzymatic activity was also assessed in the cells. A TNAP inhibitor was used to delineate its implication in the mineralization process. Results Tendon samples were obtained from 5 patients (age 69.6±5.13 years). Cells extracted from these tendons expressed collagen I, collagen III, Scleraxis and Mkx (Mohawk homeobox), as expected for tenocytes. However, Tenomodulin was very weakly expressed and lost after passage 1. These cells were able to mineralize in the OM although no mineralization was observed in the control medium. qPCR analyses showed a significant increase of TNAP and ENPP1 expression by cells cultured in OM (p Conclusions Tenocyte-like cells extracted from tendons of the rotator cuff are able to induce mineralization in an osteogenic medium. The cells express genes associated with a hypertrophic chondrocyte phenotype (TNAP, COL10 and MMP13) and TNAP seems to have a crucial role in the induced mineralization. These results suggest that tenocytes could differentiate into hypertrophic chondrocyte which induce TNAP-dependent apatite deposition in calcific tendonitis. Acknowledgements This study was supported by Inserm and the French Society of Rheumatology. Disclosure of Interest None declared.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::51240f83d4c3bae4a1881771adfcb93dTest
https://doi.org/10.1136/annrheumdis-2018-ewrr2019.99Test

المؤلفون: A. Hamel, P. Corre, Christelle Darrieutort Laffite, B. Le Goff, B. Champs

المساهمون: Hôtel-Dieu

المصدر: Journal of Stomatology, Oral and Maxillofacial Surgery
Journal of Stomatology, Oral and Maxillofacial Surgery, Elsevier Masson, 2019, 120, pp.67-70. ⟨10.1016/j.jormas.2018.10.008⟩

مصطلحات موضوعية: musculoskeletal diseases, TMJ disorders, [SDV]Life Sciences [q-bio], Imaging technic, Injections, Intra-Articular, 03 medical and health sciences, 0302 clinical medicine, Cadaver, Daily practice, Humans, Medicine, In patient, 030223 otorhinolaryngology, Ultrasonography, Interventional, Ultrasonography, Temporomandibular Joint, business.industry, 030206 dentistry, Temporomandibular Joint Disorders, medicine.disease, 3. Good health, Temporomandibular joint, In plane, medicine.anatomical_structure, Otorhinolaryngology, Surgery, Oral Surgery, business, Nuclear medicine

الوصف: Ultrasonography of the temporomandibular joint is a non-invasive imaging technic, easy to perform in daily practice. It can be used for diagnosis and to guide intra-articular injections. The objective was to validate a longitudinal in-plane US injection approach of the joint and assess its accuracy. We performed a study in 13 non-embalmed cadavers. The injection was done under real-time US guidance using a needle inserted in-plane with an angulation of 30°and positioned under the capsule until the injection was feasible without resistance. The intra-articular injection was successful in all cases and confirmed by a liquid backflow in 96% of cases. The median duration between skin puncture and the intra-articular injection was 23 seconds. Our technique allows a direct visualization of the needle throughout its course to the joint with a high accuracy. Other studies will be needed to confirm its feasibility and usefulness in patients with TMJ disorders.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1902922cc1b83d28483972a256b6e321Test
https://hal.archives-ouvertes.fr/hal-03486615/file/S2468785518302192.pdfTest

المؤلفون: Guillaume Coiffier, Sébastien Ottaviani, A. Le Pluart, Gaetane Nocturne, B. Le Goff, Christelle Darrieutort-Laffite, Denis Mulleman, Sophie Godot, Marion Couderc, Emmanuel Hoppé, Grégoire Cormier, Géraldine Bart, O. Mérot

المصدر: Revue du Rhumatisme. 87:A8

مصطلحات موضوعية: Rheumatology

الوصف: Introduction Dans une precedente etude, nous avons montre que la prevalence des complications neurologiques au cours de la spondylodiscite infectieuse (SPI) etait de 40 %. L’immobilisation rachidienne est recommandee pour prevenir la survenue de ces complications, mais avec un faible niveau de preuve dans la litterature. Le but de notre etude etait de decrire la pratique de l’immobilisation dans une large cohorte de patients atteints de SPI et d’evaluer l’association entre l’immobilisation et la survenue de complication neurologique. Patients et methodes Nous avons recrute des patients de facon prospective entre 2016 et 2019, dans 11 centres hospitaliers. Ont ete inclus les patients adultes avec SPI confirmee a l’imagerie et avec identification d’un germe, ou avec une bonne reponse a une antibiotherapie en cas de prelevement sterile. Nous avons recueilli les donnees cliniques, biologiques, d’imagerie et de traitement a l’admission et durant le suivi a 3 et 6 mois. Nous avons defini une immobilisation rachidienne stricte comme une immobilisation pour corset rigide pour une duree minimale de 6 semaines. Resultats 250 patients ont ete inclus. L’âge moyen etait de 66,7 ± 15 ans, principalement des hommes (67,2 %, n = 168). La duree mediane des symptomes avant le diagnostic etait de 25 (0 a 427) jours. Au diagnostic, 25,6 % des patients (n = 64) presentaient des signes neurologiques mineurs et 9,2 % (n = 23) une atteinte neurologique majeure (deficit moteur, trouble sphincterien). Au cours du suivi, l’apparition de signes mineurs est survenue chez 9,2 % (n = 23) des patients, les complications majeures sont apparues dans 6,8 % (n = 17) des cas, la duree mediane de survenue des complications majeures etait de 11 (1 a 45) jours. Une immobilisation rachidienne par corset rigide a ete prescrite dans 64,8 % (n = 162) des cas et pour 44 % (n = 110) des patients pour une duree minimale de 6 semaines. En analyse multivariee, les facteurs associes a la prescription d’une immobilisation stricte etaient : le centre (p = 0,035), le jeune âge (p = 0,005), la compression du sac dural (p = 0,005) et les signes neurologiques majeurs au diagnostic (p = 0,036). Il n’y avait pas de difference significative dans l’apparition secondaire des complications neurologiques mineures entre les patients immobilises strictement et les autres (respectivement 10,9 % (n = 12) et 5,9 % (n = 7), log Rank = 0,315), de meme pour les complications neurologiques majeures (4,5 % (n = 5) dans le groupe immobilisation stricte et 6,7 % (n = 8) chez les autres patients, log Rank = 0,913). La duree de l’immobilisation prescrite n’etait pas associee a l’apparition de complications neurologiques. Chez les patients n’ayant pas recu d’immobilisation rigide, l’effacement de l’espace sous arachnoidien etait significativement associe a l’apparition de complication neurologique majeure en analyse multivariee (p = 0,016). Il n’y avait pas de difference significative sur l’evolution fonctionnelle (score Oswestry) ou sur l’EVA douleur a 3 et 6 mois entre les 2 groupes de patients. Conclusion Dans notre etude, la majorite des patients atteints de SPI ont beneficie d’une immobilisation rachidienne, mais pour une duree variable. Il n’y avait pas de difference significative en termes d’apparition de complications neurologiques ou de pronostic fonctionnel entre les patients immobilises de facon stricte et les autres.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::d3c22ba9bc4a4ce25373c1d5dd3c0880Test
https://doi.org/10.1016/j.rhum.2020.10.017Test

المؤلفون: Benoit Le Goff, Lucie Planche, Christelle Darrieutort-Laffite, Géraldine Bart, Yves Maugars, Joëlle Glémarec

المصدر: Joint Bone Spine. 82:356-361

مصطلحات موضوعية: Anesthesia, Epidural, Male, medicine.medical_specialty, Hydrocortisone, Visual analogue scale, medicine.medical_treatment, Anti-Inflammatory Agents, Injections, Epidural, Spinal Puncture, Palpation, law.invention, Sciatica, Lumbar, Rheumatology, Randomized controlled trial, law, medicine, Clinical endpoint, Humans, Single-Blind Method, Prospective Studies, Pain Measurement, Ultrasonography, Lumbar Vertebrae, medicine.diagnostic_test, Epidural steroid injection, business.industry, Lumbosacral Region, Middle Aged, Epidural space, Surgery, Treatment Outcome, medicine.anatomical_structure, Anesthesia, Feasibility Studies, Female, medicine.symptom, business, Low Back Pain

الوصف: Ultrasound (US) is widely used in rheumatology to study and guide injection of peripheral joints. It can also provide useful information about the anatomy of the lumbar spine. Studies have shown that US examination of the spine was a useful tool to help perform epidural anaesthesia. The purpose of the study was to determine if the selection of the optimum puncture level by US may facilitate epidural steroid injection in case of presumed difficult puncture (BMI30 kg/m(2), age60 years or lumbar scoliosis).We performed a prospective randomized controlled study. Eighty patients were randomized in two groups: US group (n=40) which underwent a pre-procedure spinal US to determine the optimal lumbar level for injection or control group (n=40) for which the level of injection was determined by palpation. Primary endpoint was the pain during the procedure assessed using the Visual Analogue Scale (VAS).We found a positive correlation between depth of the epidural space and BMI (P0.001) and a negative correlation between size of the interspinous spaces and age (P0.01). Visibility of the epidural space was not altered by obesity or age. We observed a trend toward a reduction in pain intensity during the procedure in the US group compared to the control group with a mean difference at -0.94 [-1.90; 0.02] but the difference was not significant (P=0.054).US of the lumbar spine was feasible in patients with lumbar conditions even in obese and old ones and allowed the visualization of the epidural space. However, pre-procedure US examination did not reduce pain during the procedure.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19e267d8a378100ff867b09137e81d59Test
https://doi.org/10.1016/j.jbspin.2015.02.001Test

المؤلفون: Benoit Le Goff, Yves Maugars, Jean-Marie Berthelot, Christelle Darrieutort-Laffite

المصدر: Revue du Rhumatisme Monographies. 82:233-236

مصطلحات موضوعية: Rheumatology

الوصف: Resume L’echographie interventionnelle osteo-articulaire prend une place de plus en plus grande en rhumatologie. Le maniement simultane de la sonde et de l’aiguille pourrait laisser penser qu’il faut prendre des mesures de precaution d’asepsie plus grandes par rapport aux gestes realises habituellement sous reperage anatomique. Sont-elles justifiees ? Nous n’avons aucune donnee allant en ce sens et une etude randomisee est impossible du fait de la grande rarete des evenements. Nous passons en revue les differents elements de l’asepsie, concernant le patient (essentiellement la desinfection de la peau), le medecin (essentiellement le port de gants steriles ou non) et le materiel (protection de la sonde echographique et distance par rapport a l’aiguille). La procedure est probablement au moins aussi importante que les moyens. L’attention doit se porter surtout sur l’integrite de l’asepsie de l’aiguille. Il ne faut pas piquer dans le gel sous peine d’apporter des polyacrylates non biodegradables ou du propylene glycol dans les tissus. Nous ne pouvons conclure a une justification de l’inflation des moyens d’asepsie mis en œuvre lors d’un geste osteo-articulaire echoguide. Certes, pour certains gestes plus complexes et agressifs, sur certains terrains fragilises, et en fonction du lieu de pratique, on peut considerer des mesures d’asepsie plus importantes, mais de la meme maniere que pour un geste habituel. Seul un observatoire des pratiques, non seulement des procedures de reparation du dommage corporel administrees par les assurances, mais surtout prospectivement et sur une tres grande echelle au niveau national, pourra nous apporter des reponses plus precises.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::5f21914dd086a11c1f8c641546334cebTest
https://doi.org/10.1016/j.monrhu.2015.06.004Test

المؤلفون: Christelle Darrieutort-Laffite, Benoit Le Goff

المصدر: Revue du Rhumatisme Monographies. 82:196-201

مصطلحات موضوعية: Rheumatology

الوصف: Resume L’echographie musculo-squelettique est aujourd’hui largement utilisee en rhumatologie pour la prise en charge diagnostique et therapeutique des pathologies mecaniques et inflammatoires. Bien que moins utilisee en pratique courante dans l’exploration du squelette axial, l’echographie est un outil interessant pour aborder le rachis lombaire et les articulations sacro-iliaques. Ainsi, l’echographie peut etre utilisee pour le guidage des infiltrations sacro-iliaques et du rachis (epidurales, articulaires posterieures, peri-radiculaires). Elle peut egalement apporter des elements diagnostiques en faveur d’une pathologie inflammatoire des sacro-iliaques dans le cadre des spondyloarthrites. Cependant, ces structures sont profondes et parfois difficiles a explorer ; c’est pourquoi il est necessaire de bien connaitre les elements de l’echo-anatomie. Cette revue fait le point sur les donnees recentes de la litterature concernant l’apport de l’echographie dans la prise en charge des pathologies rachidiennes et des sacro-iliaques.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::28348eddc0395b3f8e4dd7e90329db78Test
https://doi.org/10.1016/j.monrhu.2015.05.001Test