المؤلفون: CHEN Yafei, LIU Qiong, GUAN Shuang, YU Yanan, LIU Jun, WANG Sicun, WANG Zhong

المصدر: Xiehe Yixue Zazhi, Vol 14, Iss 6, Pp 1155-1161 (2023)

مصطلحات موضوعية: digital twin, application method, medical field, research progress, Medicine

الوصف: With the rapid development of emerging biotechnologies such as high-throughput sequencing, multi-omics and multi-dimensional research models for biological big data have been initiated. Concurrently, the fast-evolving technologies including mathematical modeling, artificial intelligence, cloud computing, blockchain, big data, the Internet of Things, and 5G have enabled the development of digital twin. Digital twin, a model mapping of physical objects, processes, and systems in digital space, has shown great potential in the medical field. Digital twin technology can provide visualized 3D structures for human organs and systems to assist diagnosis and treatment, create a tangible 'skeleton' for data mining in genomics, metabolomics, and phenomics, and simulate processes for chronic disease management, drug development, and clinical trials, thereby advancing the medical field. The aim of the article, therefore, is to review the methods and applications of digital twin in the medical field, with the hope of providing reference for the development of medical digital twin research in China.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1674-9081Test

الوصول الحر: https://doaj.org/article/ef28ca36a4c84defbf7e2b28c93ae60cTest

المؤلفون: Chen Yafei, Ge Kangkang

المصدر: Applied Mathematics and Nonlinear Sciences, Vol 9, Iss 1 (2024)

مصطلحات موضوعية: interrupted finite elements, schrödinger’s kirchhoff poisson equation, variational principle, numerical simulation, existence, 00a73, Mathematics, QA1-939

الوصف: In this paper, we firstly explore the existence of solutions to the following linear Schrödinger Kirchhoff Poisson equation with critical exponential growth on the full space □3 by using the discontinuous finite element (DG) as well as the principle of centralized compactness: {−(a+b∫□3|∇u|2)Δu+V(x)u+φu-12uΔ(u2)=K(x)up−2u,x∈□3−Δφ=u2,x∈□3\left\{ {\matrix{{ - \left( {a + b\int_{{\square^3}} {{{\left| {\nabla u} \right|}^2}} } \right)\Delta u + V\left( x \right)u + \phi u - {1 \over 2}u\Delta \left( {{u^2}} \right) = K\left( x \right){u^{p - 2}}u,} & {x \in {\square^3}} \cr { - \Delta \phi = {u^2},} & {x \in {\square^3}} \cr } } \right., x ∈. Secondly, we make reasonable assumptions on the V, K , f functions of the equation, and use the principle of variational division to firstly obtain the corresponding energy generalization of this equation, and then we prove the corresponding generalizations of the equation satisfy the (C)c conditions. Finally, the existence of the solution of the equation is obtained by numerical simulation and then by using the Yamaji Lemma. The results show that the error of the finite element solution of the linear Schrödinger Kirchhoff Poisson equation in the spatial direction P1 reaches the optimal estimation under the L2 -parameter in an intermittent finite element numerical simulation environment, i.e., it is proved that there exist at least 1 and 3 positive solutions to the problem. The paper achieves rich research results which are informative for the solution of this class of linear differential equations.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2444-8656Test

الوصول الحر: https://doaj.org/article/287159b7bca643eaba2f497e347c3819Test

المؤلفون: Zhang, Haozhe, Zhou, Yi, Feng, Yating, Hou, Wenli, Chen, Yafei, Xing, Zengzhen, Zhang, Yifan, Wei, Qiang, Yin, Yu, Guo, Ju, Hu, Hailiang

المساهمون: National Natural Science Foundation of China, Natural Science Foundation of Guangdong Province

المصدر: Clinical and Translational Medicine ; volume 14, issue 5 ; ISSN 2001-1326 2001-1326

الوصف: Background Cyclin‐dependent kinase 12 (CDK12)‐deficient prostate cancer defines a subtype of castration‐resistant prostate cancer (CRPC) with a poor prognosis. Current therapy, including PARP inhibitors, shows minimal treatment efficacy for this subtype of CRPC, and the underlying mechanism remains elusive. Methods Based on bioinformatics analysis, we evaluated the relationship between CDK12 deficiency and prostate cancer patient's prognosis and treatment resistance. Furthermore, we used CRISPR‐Cas9 technology and mass spectrometry‐based metabolomic profiling to reveal the metabolic characteristics of CDK12‐deficient CRPC. To elucidate the specific mechanisms of CDK12 deficiency‐mediated CRPC metabolic reprogramming, we utilized cell RNA‐seq profiling and other molecular biology techniques, including cellular reactive oxygen species probes, mitochondrial function assays, ChIP‐qPCR and RNA stability analyses, to clarify the role of CDK12 in regulating mitochondrial function and its contribution to ferroptosis. Finally, through in vitro drug sensitivity testing and in vivo experiments in mice, we identified the therapeutic effects of the electron transport chain (ETC) inhibitor IACS‐010759 on CDK12‐deficient CRPC. Results CDK12‐deficient prostate cancers reprogramme cellular energy metabolism to support their aggressive progression. In particular, CDK12 deficiency enhanced the mitochondrial respiratory chain for electronic transfer and ATP synthesis to create a ferroptosis potential in CRPC cells. However, CDK12 deficiency downregulated ACSL4 expression, which counteracts the lipid oxidation stress, leading to the escape of CRPC cells from ferroptosis. Furthermore, targeting the ETC substantially inhibited the proliferation of CDK12‐deficient CRPC cells in vitro and in vivo, suggesting a potential new target for the therapy of CDK12‐deficient prostate cancer. Conclusions Our findings show that energy and lipid metabolism in CDK12‐deficient CRPC work together to drive CRPC progression and provide a ...

الإتاحة: https://doi.org/10.1002/ctm2.1678Test

المؤلفون: Chen, Yafei, Li, Xu, Zhu, Li, Wang, Yan, Gan, Liangying, Zuo, Li

المصدر: Blood Purification ; page 1-8 ; ISSN 0253-5068 1421-9735

مصطلحات موضوعية: Nephrology, Hematology, General Medicine

الوصف: Introduction: This study aimed to evaluate the predictive value of the low-frequency/high-frequency (LF/HF) ratio in all causes of death and hospitalizations in maintenance hemodialysis (MHD) patients. Methods: This is a single-center prospective study with a 48-h electrocardiograph (ECG) recording. A total of 110 patients were enrolled in the study from October 1, 2021, to September 30, 2022. ECG recordings started before initiation of the hemodialysis (HD) session and lasted for 48 h, covering the intra- as well as inter-HD period. We divided our participants into two groups based on the median value of LF/HF, one of the frequency domain parameters of heart rate variability (HRV). Patients with LF/HF <1.33 were categorized as group A and those with LF/HF ≥1.33 were group B. The endpoint of the study was a composite event of death or hospitalization. We followed all patients until the composite endpoint or the end of the study on February 28, 2023. Multivariate Cox regression was used to assess the adjusted effect of LF/HF on the composite endpoint. Results: Patients in group A were older and the number of patients with diabetes was more than that of group B. With regards to the laboratory data, group A had lower serum creatinine and uric acid and higher ferritin and NT-ProBNP. In the index HD session, systolic blood pressure was higher but diastolic blood pressure was significantly lower in group A. During the median follow-up period of 8.8 (7.6–9.8) months, 27 hospitalizations and 10 deaths were documented. Increased LF/HF ratio was an independent protective factor of composite endpoint events (HR = 0.357, 95% CI: 0.162–0.790, p = 0.011). Conclusion: Risks of mortality and hospitalizations are higher among HD patients having decreased LF/HF ratios. LF/HF in the 48-h recording can be considered as a prognostic factor for risk stratification in HD patients.

الإتاحة: https://doi.org/10.1159/000536538Test

المؤلفون: Wang, Guoyu, Chen, Yafei, Liu, Xinxin, Ma, Siyi, Jiang, Min

المصدر: Medicine ; volume 103, issue 3, page e36857 ; ISSN 0025-7974 1536-5964

الوصف: Object: This study aimed to investigate global research advances and hot trends in prediabetes in the last decade based on a bibliometric analysis of publications. Publications from 2013 to 2022 were retrieved from the Web of Science Core Collection database through a topic search. With the use of CiteSpace, VOS viewer, and Bibliometrix R software packages, the number of publications, production categories, countries/regions, institutions, authors, journals, references, and keywords were comprehensively analyzed to sort out the hot spots and directions of prediabetes and predict the future research directions. A total of 13,223 papers were recruited for this study by the end of March 3, 2023. A generally increasing trend was observed in the number of annual publications. PLOS ONE (journal), USA (national), and the University of Copenhagen (institutional) published the most papers in this research area. The top 3 contributor authors were Tuomilehto Jaakko, Rathmann Wolfgang, and Peters Annette. “Intestinal microbiota” (2020–2022) was the most populated keyword in terms of intensity, and “biomarkers,” “gut microbiota,” and “metabolomics” were the most populated keywords in the last 3 years. “Prediabetes: a high-risk state for diabetes development-2012” was the strongest burst reference. This study summarized the research hotspots and trends in prediabetes research in the last decade. Frontier research can be found in the journal Diabetes Care and Journal of Clinical Endocrinology Metabolism. Prediabetes research focuses on preventing risk factors to reduce the prevalence of prediabetes, and current research hotspots focus on gut microbes and metabolism-related biomarkers.

الإتاحة: https://doi.org/10.1097/md.0000000000036857Test

المؤلفون: Li, Honglin, Paka Lubamba, Grace, Zhang, Gaowei, Li, Chunjie, Han, Bo, Gao, Ning, Wang, Lei, Chen, Yafei, Yan, Bing

المصدر: Journal of Craniofacial Surgery ; volume 35, issue 4, page e371-e374 ; ISSN 1049-2275

الوصف: Purpose: Iatrogenic lip injury may occur during oral and maxillofacial surgical procedures. This study aimed to evaluate the effect of oral retractors on iatrogenic lip injury prevention during intraoral procedures of oral and maxillofacial surgery. Methods: We conducted a randomized controlled trial and included patients who underwent intraoral procedures of oral and maxillofacial surgery. Patients were randomly allocated to receive oral retractor (intervention group) or traditional procedure without lip protection (control group). The incidence of lip injury was the outcome variable. Other study variables included surgical time and satisfaction of patients and surgeons with treatment experience evaluated by visual analog scale (VAS). Student t test and χ 2 test were used to compare both groups’ variables and measure the relationship between the predictor variable and the outcome variable. P <0.05 was considered significant for all analyses. Results: A total of 114 patients were included, with 56 allocated to intervention group and 58 to control group. The results showed that the application of an oral retractor did not significantly increase surgical time ( P =0.318). A total of 12 patients had lip injury, with 1 in the intervention group and 11 in the control group ( P =0.003). For the assessment of satisfaction with treatment experience, the intervention group had significantly higher VAS scores for doctors and patients ( P <0.05). Conclusions: We found that the oral retractor was a good tool for iatrogenic lip injury prevention in oral and maxillofacial surgical procedures and could be considered in clinical treatment.

الإتاحة: https://doi.org/10.1097/scs.0000000000010112Test

المؤلفون: Xu, Shuyin, Ju, Yumeng, Wei, Xiyu, Ou, Wenwen, Ma, Mohan, Lv, Guanyi, Zhao, Xiaotian, Qin, Yaqi, Li, Yunjing, Li, Liang, Huang, Mei, Yang, Siqi, Lu, Yimei, Chen, Yafei, Liu, Junwu, Liu, Jin, Liu, Bangshan, Zhang, Yan

المساهمون: the National Key Research and Development Program of China, Postgraduate Innovative Project of Central South University, National Natural Science Foundation of China, the science and technology innovation Program of Hunan Province, the Central South University Postdoctoral Programme

المصدر: General Psychiatry ; volume 37, issue 2, page e101225 ; ISSN 2517-729X

الوصف: Background The co-occurrence of depression and anxiety among adolescents is typically associated with suicide ideation. Aims The study aimed to investigate the symptom-level relationship between suicide ideation and the comorbidity of depression and anxiety. Methods 1501 adolescents aged 12–19 years were assessed using the Patient Health Questionnaire (PHQ-9) and the Generalized Anxiety Disorder Scale, and 716 adolescents who scored ≥5 on both scales were selected as participants. Network analysis was used to identify the network structure of depressive symptoms and anxiety symptoms. Participants were categorised into either the suicide ideation or non-suicide ideation groups based on their scoring on the suicide-related item in PHQ-9. A comparison was made between the depression–anxiety symptom networks of the two groups. Results ‘Restlessness’, ‘sad mood’ and ‘trouble relaxing’ were the most prominent central symptoms in the depression–anxiety symptom network, and ‘restlessness’, ‘nervousness’ and ‘reduced movement’ were the bridge symptoms in this network. ‘Sad mood’ was found to be directly related to ‘suicide ideation’ with the highest variance. The network structure was significantly different in properties between the suicide ideation group and the non-suicide ideation group, with ‘restlessness’ and ‘sad mood’ exhibiting significantly higher influence in the network of the suicide ideation group than that in the non-suicide ideation group. Conclusion Restlessness and sad mood could be targeted for the intervention of depression–anxiety symptoms among adolescents with suicide ideation.

الإتاحة: https://doi.org/10.1136/gpsych-2023-101225Test

المؤلفون: Peng, Hongxin, Chen, Pinpin, Guo, Rui, Zhou, Zheng, Chen, Yafei, Xu, Ping, Su, Huawei, Xiao, Yuncai, Jiang, Hui

المصدر: Frontiers in Microbiology ; volume 15 ; ISSN 1664-302X

مصطلحات موضوعية: Microbiology (medical), Microbiology

الوصف: This study investigated the effects of defective pear fermentation (DPF) diets on growth performance and gastrointestinal microbial communities in 60 healthy male small-tailed Han sheep, aged 90 days. The sheep were randomly divided into four groups, each consisting of three replicates with five sheep per replicate. Initially, all groups received a basal diet for seven days during the adaptation stage. Subsequently, for 60 days, group C (control) was fed a basal diet, group X received a basal diet with 2% DPF, group Y had a basal diet with 4% DPF, and group Z was fed a basal diet with 6% DPF. The results indicated that group Y experienced a significant increase in average daily gain (ADG) and average daily feed intake (ADFI). The addition of DPF significantly elevated the levels of GSH-Px and notably reduced MDA content compared to group C. Analysis of gastrointestinal microbiota showed that groups receiving DPF had increased relative abundances of Lachnospiraceae_NK3A20_group, norank_f p-2534-18B5_gut_group, Acetitomaculum, Actinobacteriota, Bacteroidota and Ruminococcus_gauvreauii_group , and decreased abundances of Proteobacteria, Prevotella, Staphylococcus , and Psychrobacter compared to group C. Group X exhibited the highest relative abundance of Olsenella , while group Y showed a significant increase in unclassified_f Lachnospiraceae compared to the other groups. Bacterial function prediction indicated that pathways related to energy metabolism were more prevalent in group X and Y. This study preliminarily confirms the feasibility of using DPF as feed additives, providing a foundation for further research and evaluation of DPF's application in animal production.

الإتاحة: https://doi.org/10.3389/fmicb.2024.1358033Test

المؤلفون: Chen, Jiaxian, Yang, Lan, Chen, Yafei, Yuan, Wei, Chen, Yao, Liang, Hong, Miao, Maohua, He, Gengsheng, Wang, Ziliang

المساهمون: National Key Research and Development Program of China, National Natural Science Foundation of China, Science and Technology Commission of Shanghai Municipality

المصدر: Frontiers in Endocrinology ; volume 15 ; ISSN 1664-2392

مصطلحات موضوعية: Endocrinology, Diabetes and Metabolism

الوصف: Background Kisspeptin has been indicated to be a biomarker of fetal growth. Although some evidence suggested that maternal kisspeptin concentrations in early pregnancy were associated with increased fetal growth, studies are still limited and the effect of kisspeptin in late pregnancy remains unknown. This study aimed to investigate the associations between maternal kisspeptin in late pregnancy and fetal growth. Methods Based on the Shanghai-Minhang Birth Cohort study, 724 mother-neonate pairs were included in this study. We measured maternal kisspeptin concentrations in the urine samples collected in late pregnancy and neonatal anthropometric indices at birth. The associations between maternal kisspeptin and neonatal anthropometry were investigated using multiple linear regression models. Results Higher maternal urinary kisspeptin concentrations were associated with lower neonatal birth weight, head circumference, upper arm circumference, abdominal skinfold thickness, triceps skinfold thickness, and back skinfold thickness. The inverse associations were more pronounced for the highest kisspeptin levels versus the lowest. These patterns were consistent in analyses stratified by neonatal sex, with notably stable associations between maternal kisspeptin concentrations and skinfold thickness. Conclusion The present study suggested that maternal kisspeptin concentrations in late pregnancy might be inversely associated with fetal growth. The physiological mechanisms of maternal kisspeptin might differ from those in early pregnancy. Further studies are required to assess associations between maternal kisspeptin and energy homeostasis and explore the physiological roles of kisspeptin in late pregnancy.

الإتاحة: https://doi.org/10.3389/fendo.2024.1257248Test

المؤلفون: Yin, Hong, Chen, Ziqiang, Chen, Yafei, Tan, Hua, Wang, Mingyuan, He, Donglin, Ouyang, Ping, Gong, Haifeng

المساهمون: Chongqing Municipal Science and Technology Bureau, Chongqing Municipal Education Commission, Natural Science Foundation of Chongqing Municipality, National Natural Science Foundation of China

المصدر: ACS Omega ; ISSN 2470-1343 2470-1343

الإتاحة: https://doi.org/10.1021/acsomega.4c01032Test