المؤلفون: Escartin, C, Galea, E, Lakatos, A, O'Callaghan, JP, Petzold, GC, Serrano-Pozo, A, Steinhäuser, C, Volterra, A, Carmignoto, G, Agarwal, A, Allen, NJ, Araque, A, Barbeito, L, Barzilai, A, Bergles, DE, Bonvento, G, Butt, AM, Chen, W-T, Cohen-Salmon, M, Cunningham, C, Deneen, B, De Strooper, B, Díaz-Castro, B, Farina, C, Freeman, M, Gallo, V, Goldman, JE, Goldman, SA, Götz, M, Gutiérrez, A, Haydon, PG, Heiland, DH, Hol, EM, Holt, MG, Iino, M, Kastanenka, KV, Kettenmann, H, Khakh, BS, Koizumi, S, Lee, CJ, Liddelow, SA, MacVicar, BA, Magistretti, P, Messing, A, Mishra, A, Molofsky, AV, Murai, KK, Norris, CM, Okada, S, Oliet, SHR, Oliveira, JF, Panatier, A, Parpura, V, Pekna, M, Pekny, M, Pellerin, L, Perea, G, Pérez-Nievas, BG, Pfrieger, FW, Poskanzer, KE, Quintana, FJ, Ransohoff, RM, Riquelme-Perez, M, Robel, S, Rose, CR, Rothstein, JD, Rouach, N, Rowitch, DH, Semyanov, A, Sirko, S, Sontheimer, H, Swanson, RA, Vitorica, J, Wanner, I-B, Wood, LB, Wu, J, Zheng, B, Zimmer, ER, Zorec, R, Sofroniew, MV, Verkhratsky, A

المصدر: Nature Neuroscience , 24 pp. 312-325. (2021)

مصطلحات موضوعية: Astrocyte, Cellular neuroscience, Diseases of the nervous system, Molecular neuroscience, Regeneration and repair in the nervous system

الوصف: Reactive astrocytes are astrocytes undergoing morphological, molecular, and functional remodeling in response to injury, disease, or infection of the CNS. Although this remodeling was first described over a century ago, uncertainties and controversies remain regarding the contribution of reactive astrocytes to CNS diseases, repair, and aging. It is also unclear whether fixed categories of reactive astrocytes exist and, if so, how to identify them. We point out the shortcomings of binary divisions of reactive astrocytes into good-vs-bad, neurotoxic-vs-neuroprotective or A1-vs-A2. We advocate, instead, that research on reactive astrocytes include assessment of multiple molecular and functional parameters-preferably in vivo-plus multivariate statistics and determination of impact on pathological hallmarks in relevant models. These guidelines may spur the discovery of astrocyte-based biomarkers as well as astrocyte-targeting therapies that abrogate detrimental actions of reactive astrocytes, potentiate their neuro- and glioprotective actions, and restore or augment their homeostatic, modulatory, and defensive functions.

وصف الملف: text

العلاقة: https://discovery.ucl.ac.uk/id/eprint/10122501/1/Whitepaper-accepted-version-corr-Fev2021.pdfTest; https://discovery.ucl.ac.uk/id/eprint/10122501Test/

الإتاحة: https://discovery.ucl.ac.uk/id/eprint/10122501/1/Whitepaper-accepted-version-corr-Fev2021.pdfTest
https://discovery.ucl.ac.uk/id/eprint/10122501Test/

المؤلفون: Mastrogiacomo, R, Trigilio, G, Devroye, C, Dautan, D, Ferretti, V, Losi, G, Caffino, L, Orso, G, Marotta, R, Maltese, F, Vitali, E, Piras, G, Forgiarini, A, Pacinelli, G, Lia, A, Rothmond, DA, Waddington, JL, Drago, F, Fumagalli, F, De Luca, MA, Leggio, GM, Carmignoto, G, Weickert, CS, Manago, F, Papaleo, F

المساهمون: Mastrogiacomo, R, Trigilio, G, Devroye, C, Dautan, D, Ferretti, V, Losi, G, Caffino, L, Orso, G, Marotta, R, Maltese, F, Vitali, E, Piras, G, Forgiarini, A, Pacinelli, G, Lia, A, Rothmond, Da, Waddington, Jl, Drago, F, Fumagalli, F, De Luca, Ma, Leggio, Gm, Carmignoto, G, Weickert, C, Manago, F, Papaleo, F

الوصف: The mechanisms underlying the dichotomic cortical/basal ganglia dopaminergic abnormalities in schizophrenia are unclear. Astrocytes are important non-neuronal modulators of brain circuits, but their role in dopaminergic system remains poorly explored. Microarray analyses, immunohistochemistry, and two-photon laser scanning microscopy revealed that Dys1 hypofunction increases the reactivity of astrocytes, which express only the Dys1A isoform. Notably, behavioral and electrochemical assessments in mice selectively lacking the Dys1A isoform unraveled a more prominent impact of Dys1A in behavioral and dopaminergic/D2 alterations related to basal ganglia, but not cortical functioning. Ex vivo electron microscopy and protein expression analyses indicated that selective Dys1A disruption might alter intracellular trafficking in astrocytes, but not in neurons. In agreement, Dys1A disruption only in astrocytes resulted in decreased motivation and sensorimotor gating deficits, increased astrocytic dopamine D2 receptors and decreased dopaminergic tone within basal ganglia. These processes might have clinical relevance because the caudate, but not the cortex, of patients with schizophrenia shows a reduction of the Dys1A isoform. Therefore, we started to show a hitherto unknown role for the Dys1A isoform in astrocytic-related modulation of basal ganglia behavioral and dopaminergic phenotypes, with relevance to schizophrenia.

وصف الملف: ELETTRONICO

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/35821415; info:eu-repo/semantics/altIdentifier/wos/WOS:000823357600001; journal:MOLECULAR PSYCHIATRY; http://hdl.handle.net/11577/3454151Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85134262464

الإتاحة: https://doi.org/10.1038/s41380-022-01683-8Test
http://hdl.handle.net/11577/3454151Test

المؤلفون: Grumelli, C, Berghuis, P, Pozzi, D, Caleo, M, Antonucci, F, Bonanno, G, Carmignoto, G, Dobszay, MB, Harkany, T, Matteoli, M, Verderio, C

المصدر: Molecular and cellular neurosciences. 39(3):314-323

مصطلحات موضوعية: Medicin och hälsovetenskap

الوصول الحر: http://kipublications.ki.se/Default.aspx?queryparsed=id:117843024Test

المؤلفون: MERIGHI, Adalberto, CARMIGNOTO G, GOBBO S, LOSSI, Laura, SALIO, Chiara, VERGNANO AM, ZONTA M.

المساهمون: MERIGHI A, CARMIGNOTO G, GOBBO S, LOSSI L, SALIO C, VERGNANO AM, ZONTA M

مصطلحات موضوعية: Neurotrofine, NGF, BDNF, Dolore, Neuroanatomia, Neurofisiologia

الوصف: Neurotrophins are a well-known family of growth factors for the central and peripheral nervous systems. In the course of the last years, several lines of evidence converged to indicate that some members of the family, particularly NGF and BDNF, also participate in structural and functional plasticity of nociceptive pathways within the dorsal root ganglia and spinal cord. A subpopulation of small-sized dorsal root ganglion neurons is sensitive to NGF and responds to peripheral NGF stimulation with up-regulation of BDNF synthesis and increased anterograde transport to the dorsal horn. In the latter, release of BDNF appears to modulate or even mediate nociceptive sensory inputs and pain hypersensitivity. We summarize here the status of the art on the role of neurotrophins in nociceptive pathways, with special emphasis on short-term synaptic and intracellular events that are mediated by this novel class of neuromessengers in the dorsal horn. Under this perspective we review the findings obtained through an array of techniques in naive and transgenic animals that provide insight into the modulatory mechanisms of BDNF at central synapses. We also report on the results obtained after immunocytochemistry, in situ hybridization, and monitoring intracellular calcium levels by confocal microscopy, that led to hypothesize that also NGF might have a direct central effect in pain modulation. Although it is unclear whether or not NGF may be released at dorsal horn endings of certain nociceptors in vivo, we believe that these findings offer a clue for further studies aiming to elucidate the putative central effects of NGF and other neurotrophins in nociceptive pathways.

العلاقة: info:eu-repo/semantics/altIdentifier/wos/WOS:000189432300019; ispartofseries:Progress in Brain Research; ispartofbook:NGF and Related Molecules in Health and Disease; volume:146; firstpage:291; lastpage:321; numberofpages:31; http://hdl.handle.net/2318/1568Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0346849770

الإتاحة: http://hdl.handle.net/2318/1568Test

المؤلفون: Scheggia D., Manago F., Maltese F., Bruni S., Nigro M., Dautan D., Latuske P., Contarini G., Gomez-Gonzalo M., Requie L. M., Ferretti V., Castellani G., Mauro D., Bonavia A., Carmignoto G., Yizhar O., Papaleo F.

المساهمون: Scheggia, D., Manago, F., Maltese, F., Bruni, S., Nigro, M., Dautan, D., Latuske, P., Contarini, G., Gomez-Gonzalo, M., Requie, L. M., Ferretti, V., Castellani, G., Mauro, D., Bonavia, A., Carmignoto, G., Yizhar, O., Papaleo, F.

مصطلحات موضوعية: Affect, Animal, Interneuron, Male, Mice, Prefrontal Cortex, Somatostatin, Social Behavior

الوصف: The prefrontal cortex (PFC) is implicated in processing of the affective state of others through non-verbal communication. This social cognitive function is thought to rely on an intact cortical neuronal excitatory and inhibitory balance. Here combining in vivo electrophysiology with a behavioral task for affective state discrimination in mice, we show a differential activation of medial PFC (mPFC) neurons during social exploration that depends on the affective state of the conspecific. Optogenetic manipulations revealed a double dissociation between the role of interneurons in social cognition. Specifically, inhibition of mPFC somatostatin (SOM+), but not of parvalbumin (PV+) interneurons, abolishes affective state discrimination. Accordingly, synchronized activation of mPFC SOM+ interneurons selectively induces social discrimination. As visualized by in vivo single-cell microendoscopic Ca2+ imaging, an increased synchronous activity of mPFC SOM+ interneurons, guiding inhibition of pyramidal neurons, is associated with affective state discrimination. Our findings provide new insights into the neurobiological mechanisms of affective state discrimination.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/31844317; info:eu-repo/semantics/altIdentifier/wos/WOS:000507601600006; volume:23; issue:1; firstpage:47; lastpage:60; numberofpages:14; journal:NATURE NEUROSCIENCE; https://hdl.handle.net/11573/1415989Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85076929984

الإتاحة: https://doi.org/10.1038/s41593-019-0551-8Test
https://hdl.handle.net/11573/1415989Test

المؤلفون: Escartin, C., Galea, E., Lakatos, A., O'Callaghan, J.P., Petzold, G.C., Serrano-Pozo, A., Steinhäuser, C., Volterra, A., Carmignoto, G., Agarwal, A., Allen, N.J., Araque, A., Barbeito, L., Barzilai, A., Bergles, D.E., Bonvento, G., Butt, A.M., Chen, W.T., Cohen-Salmon, M., Cunningham, C., Deneen, B., De Strooper, B., Díaz-Castro, B., Farina, C., Freeman, M., Gallo, V., Goldman, J.E., Goldman, S.A., Götz, M., Gutiérrez, A., Haydon, P.G., Heiland, D.H., Hol, E.M., Holt, M.G., Iino, M., Kastanenka, K.V., Kettenmann, H., Khakh, B.S., Koizumi, S., Lee, C.J., Liddelow, S.A., MacVicar, B.A., Magistretti, P., Messing, A., Mishra, A., Molofsky, A.V., Murai, K.K., Norris, C.M., Okada, S., Oliet, S.H.R., Oliveira, J.F., Panatier, A., Parpura, V., Pekna, M., Pekny, M., Pellerin, L., Perea, G., Pérez-Nievas, B.G., Pfrieger, F.W., Poskanzer, K.E., Quintana, F.J., Ransohoff, R.M., Riquelme-Perez, M., Robel, S., Rose, C.R., Rothstein, J.D., Rouach, N., Rowitch, D.H., Semyanov, A., Sirko, S., Sontheimer, H., Swanson, R.A., Vitorica, J., Wanner, I.B., Wood, L.B., Wu, J., Zheng, B., Zimmer, E.R., Zorec, R., Sofroniew, M.V., Verkhratsky, A.

مصطلحات موضوعية: Function and Dysfunction of the Nervous System, Topic 2: Molecular Processes and Therapies

الوصف: Reactive astrocytes are astrocytes undergoing morphological, molecular, and functional remodeling in response to injury, disease, or infection of the CNS. Although this remodeling was first described over a century ago, uncertainties and controversies remain regarding the contribution of reactive astrocytes to CNS diseases, repair, and aging. It is also unclear whether fixed categories of reactive astrocytes exist and, if so, how to identify them. We point out the shortcomings of binary divisions of reactive astrocytes into good-vs-bad, neurotoxic-vs-neuroprotective or A1-vs-A2. We advocate, instead, that research on reactive astrocytes include assessment of multiple molecular and functional parameters-preferably in vivo-plus multivariate statistics and determination of impact on pathological hallmarks in relevant models. These guidelines may spur the discovery of astrocyte-based biomarkers as well as astrocyte-targeting therapies that abrogate detrimental actions of reactive astrocytes, potentiate their neuro- and glioprotective actions, and restore or augment their homeostatic, modulatory, and defensive functions.

العلاقة: Reactive astrocyte nomenclature, definitions, and future directions. Escartin, C. and Galea, E. and Lakatos, A. and O'Callaghan, J.P. and Petzold, G.C. and Serrano-Pozo, A. and Steinhäuser, C. and Volterra, A. and Carmignoto, G. and Agarwal, A. and Allen, N.J. and Araque, A. and Barbeito, L. and Barzilai, A. and Bergles, D.E. and Bonvento, G. and Butt, A.M. and Chen, W.T. and Cohen-Salmon, M. and Cunningham, C. and Deneen, B. and De Strooper, B. and Díaz-Castro, B. and Farina, C. and Freeman, M. and Gallo, V. and Goldman, J.E. and Goldman, S.A. and Götz, M. and Gutiérrez, A. and Haydon, P.G. and Heiland, D.H. and Hol, E.M. and Holt, M.G. and Iino, M. and Kastanenka, K.V. and Kettenmann, H. and Khakh, B.S. and Koizumi, S. and Lee, C.J. and Liddelow, S.A. and MacVicar, B.A. and Magistretti, P. and Messing, A. and Mishra, A. and Molofsky, A.V. and Murai, K.K. and Norris, C.M. and Okada, S. and Oliet, S.H.R. and Oliveira, J.F. and Panatier, A. and Parpura, V. and Pekna, M. and Pekny, M. and Pellerin, L. and Perea, G. and Pérez-Nievas, B.G. and Pfrieger, F.W. and Poskanzer, K.E. and Quintana, F.J. and Ransohoff, R.M. and Riquelme-Perez, M. and Robel, S. and Rose, C.R. and Rothstein, J.D. and Rouach, N. and Rowitch, D.H. and Semyanov, A. and Sirko, S. and Sontheimer, H. and Swanson, R.A. and Vitorica, J. and Wanner, I.B. and Wood, L.B. and Wu, J. and Zheng, B. and Zimmer, E.R. and Zorec, R. and Sofroniew, M.V. and Verkhratsky, A. Nature Neuroscience 24 (3): 312-325. March 2021

الإتاحة: https://doi.org/10.1038/s41593-020-00783-4Test
http://edoc.mdc-berlin.de/20008Test/
https://edoc.mdc-berlin.de/20008Test/

المؤلفون: Araque, A., Carmignoto, G., Haydon, P.G., Oliet, S.H., Robitaille, R., Volterra, A.

المصدر: Neuron, vol. 81, no. 4, pp. 728-739

مصطلحات موضوعية: Animals, Astrocytes/physiology, Cell Communication/physiology, Humans, Neuronal Plasticity/physiology, Neurons/physiology, Synapses/metabolism, Synaptic Transmission/physiology

الوصف: The identification of the presence of active signaling between astrocytes and neurons in a process termed gliotransmission has caused a paradigm shift in our thinking about brain function. However, we are still in the early days of the conceptualization of how astrocytes influence synapses, neurons, networks, and ultimately behavior. In this Perspective, our goal is to identify emerging principles governing gliotransmission and consider the specific properties of this process that endow the astrocyte with unique functions in brain signal integration. We develop and present hypotheses aimed at reconciling confounding reports and define open questions to provide a conceptual framework for future studies. We propose that astrocytes mainly signal through high-affinity slowly desensitizing receptors to modulate neurons and perform integration in spatiotemporal domains complementary to those of neurons.

وصف الملف: application/pdf

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/24559669; info:eu-repo/semantics/altIdentifier/eissn/1097-4199; info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_028C9626FD9E9; https://serval.unil.ch/notice/serval:BIB_028C9626FD9ETest; urn:issn:0896-6273; https://serval.unil.ch/resource/serval:BIB_028C9626FD9E.P001/REF.pdfTest; http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_028C9626FD9E9Test

الإتاحة: https://doi.org/10.1016/j.neuron.2014.02.007Test
https://serval.unil.ch/notice/serval:BIB_028C9626FD9ETest
https://serval.unil.ch/resource/serval:BIB_028C9626FD9E.P001/REF.pdfTest
http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_028C9626FD9E9Test

المؤلفون: Maffei, L., Carmignoto, G., Perry, V. H., Candeo, P., Ferrari, G.

المصدر: Proceedings of the National Academy of Sciences of the United States of America, 1990 Mar 01. 87(5), 1855-1859.

الوصول الحر: https://www.jstor.org/stable/2353944Test

المؤلفون: Greotti E., Fortunati I., Pendin D., Ferrante C., Galla L., Zentilin L., Giacca M., Kaludercic N., Di Sante M., Mariotti L., Lia A., Gomez-Gonzalo M., Sessolo M., Carmignoto G., Bozio R., Pozzan T.

المساهمون: Greotti, E., Fortunati, I., Pendin, D., Ferrante, C., Galla, L., Zentilin, L., Giacca, M., Kaludercic, N., Di Sante, M., Mariotti, L., Lia, A., Gomez-Gonzalo, M., Sessolo, M., Carmignoto, G., Bozio, R., Pozzan, T.

مصطلحات موضوعية: Biological Sciences Tool, Cell Biology, Optical Imaging

الوصف: Genetically Encoded Ca2+ Indicators (GECIs) are extensively used to study organelle Ca2+ homeosta- sis, although some available probes are still plagued by a number of problems, e.g., low fluorescence intensity, partial mistargeting, and pH sensitivity. Furthermore, in the most commonly used mitochon- drial Fo ̈ rster Resonance Energy Transfer based-GECIs, the donor protein ECFP is characterized by a double exponential lifetime that complicates the fluorescence lifetime analysis. We have modified the cytosolic and mitochondria-targeted Cameleon GECIs by (1) substituting the donor ECFP with mCerulean3, a brighter and more stable fluorescent protein with a single exponential lifetime; (2) extensively modifying the constructs to improve targeting efficiency and fluorescence changes caused by Ca2+ binding; and (3) inserting the cDNAs into adeno-associated viral vectors for in vivo expression. The probes have been thoroughly characterized in situ by fluorescence microscopy and Fluorescence Lifetime Imaging Microscopy, and examples of their ex vivo and in vivo applications are described.

وصف الملف: STAMPA

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/31203189; info:eu-repo/semantics/altIdentifier/wos/WOS:000473321700027; volume:16; firstpage:340; lastpage:355; numberofpages:16; journal:ISCIENCE; https://hdl.handle.net/11571/1467702Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85067210752; https://www.cell.com/iscience/pdf/S2589-0042Test(19)30168-3.pdf

الإتاحة: https://doi.org/10.1016/j.isci.2019.05.031Test
https://hdl.handle.net/11571/1467702Test
https://www.cell.com/iscience/pdf/S2589-0042Test(19)30168-3.pdf

المؤلفون: Majnaric, M., Zonta, M., Lia, A., Carmignoto, G.

المصدر: European Neuropsychopharmacology ; volume 31, page S12 ; ISSN 0924-977X

مصطلحات موضوعية: Pharmacology (medical), Biological Psychiatry, Psychiatry and Mental health, Neurology (clinical), Neurology, Pharmacology

الإتاحة: https://doi.org/10.1016/j.euroneuro.2019.12.017Test
https://api.elsevier.com/content/article/PII:S0924977X1931781X?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0924977X1931781X?httpAccept=text/plainTest