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1دورية أكاديمية
المؤلفون: Heitor Bernardes Pereira Delfino, Marcela Augusta de Souza Pinhel, Flávia Campos Ferreira, Carolina Ferreira Nicoletti, Sofia Teixeira Prates de Oliveira, Lidia Barbieri Belarmino Baumgartner, Caroline Rossi Welendorf, Thaís Alves de Azevedo Chaves Pastore, Carolina Hunger Malek-Zadeh, Ligia Moriguchi Watanabe, Natália Yumi Noronha, Wilson Salgado Junior, Carla Barbosa Nonino
المصدر: Obesities, Vol 4, Iss 2, Pp 55-65 (2024)
مصطلحات موضوعية: binge eating disorder, obesity, bariatric surgery, anxiety, depression, Food processing and manufacture, TP368-456, Nutritional diseases. Deficiency diseases, RC620-627
الوصف: Obesity is a complex disease with a multifactorial etiology and could be associated with psychiatric disorders, such as Binge Eating Disorder (BED), characterized by recurrent episodes of binge eating in the absence of compensatory behaviors. The present study aimed to analyze anthropometric and body composition changes over five years after bariatric surgery in patients diagnosed or not diagnosed with BED, depression, and anxiety in the preoperative period. One hundred and eighteen patients undergoing bariatric surgery were evaluated and divided into two groups according to the presence or absence of BED. The patients were submitted to anthropometric and body composition evaluation. We also analyzed BED diagnosis, depression, and anxiety according to the DSM-5 and validated questionnaires. The Kolmogorov–Smirnov, t-test, Fisher’s, and chi-square tests were used for statistical analysis. Over five years after bariatric surgery, only the BED group exhibited an increased weight and BMI (p < 0.05). In the preoperative period, patients with BED had severe depression (13,11%, p = 0.0079) and a higher frequency of moderate (22.95%, p < 0.01) or severe (14.75%, p < 0.01) anxiety. In conclusion, patients with BED had more intense symptoms of depression and anxiety in the preoperative period, and this disorder may persist in the postoperative period of bariatric surgery, contributing to weight gain and increased BMI.
وصف الملف: electronic resource
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2دورية أكاديمية
المؤلفون: Natália Yumi Noronha, Luísa Maria Diani, Guilherme da Silva Rodrigues, Isabela Harumi Yonehara Noma, Vanessa Aparecida Batista Pereira, Marcela Augusta de Souza Pinhel, Lígia Moriguchi Watanabe, Déborah Araújo Morais, Fernando Barbosa, Carla Barbosa Nonino
المصدر: Obesities, Vol 4, Iss 2, Pp 85-92 (2024)
مصطلحات موضوعية: cobalt, DNA methylation, obesity, inductively coupled plasma mass spectrometry, epigenetics, Food processing and manufacture, TP368-456, Nutritional diseases. Deficiency diseases, RC620-627
الوصف: Obesity, a multifactorial disorder, has been associated with alterations in metal metabolismand epigenetic modifications. This pilot case–control study aimed to investigate serum cobalt concentrations and associated DNA methylation patterns in women with obesity. Serum cobalt levels were measured using inductively coupled plasma mass spectrometry (ICP-MS), revealing significantly higher cobalt concentrations in participants with normal weight than in participants with obesity. Additionally, DNA methylation analysis identified differentially methylated positions (DMPs) associated with cobalt exposure, and DMPs between groups highlighted hypomethylation in the top DMPs in individuals with obesity. Functional enrichment analysis of these DMPs unveiled potential pathways implicated in apoptosis, cancer, and metabolic signaling, warranting further investigation into the mechanistic links. This study provides preliminary insights into the interplay between cobalt exposure, DNA methylation, and potential implications for obesity management.
وصف الملف: electronic resource
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3دورية أكاديمية
المؤلفون: Natália Yumi Noronha, Luzânia dos Santos Martins, Lígia Moriguchi Watanabe, Marcela Augusta de Souza Pinhel, Guilherme da Silva Rodrigues, Isabelle Mello Schneider, Carolina Ferreira Nicoletti, Heitor Bernardes Pereira Delfino, Daniela Carlos, Carla Barbosa Nonino
المصدر: Obesities, Vol 4, Iss 2, Pp 45-54 (2024)
مصطلحات موضوعية: home-based intervention, obesity, dietary intake, women, Food processing and manufacture, TP368-456, Nutritional diseases. Deficiency diseases, RC620-627
الوصف: Background: Previous studies have suggested that changes in the composition of intestinal microbiota may be influenced by dietary quality. Objective: This study aimed to assess the impact of a hypocaloric diet on the relationship between microbiota and clinical/dietary variables. Methods: This was a longitudinal study. Ten women with obesity (Obese Group, ObG) participated in an 8-week home-based hypocaloric diet intervention. Anthropometric, dietary intake, biochemical, and gut microbiota assessments were conducted before and after the intervention. Microbiota relative abundance was determined using real-time PCR in triplicate. Results: In the ObG, the hypocaloric diet intervention led to significant weight loss (before: 119.5 ± 10.3 kg; after: 114.9 ± 10.2 kg; p = 0.003). Following the intervention, positive correlations were observed between nutrient intake and phyla composition: Actinobacteria phylum with fibers (r = 0.787; p = 0.012), Firmicutes phylum with proteins (r = 0.736; p = 0.024), and Proteobacteria phylum with lipids (r = 0.669; p = 0.049). Conclusions: The hypocaloric diet intervention improved health parameters associated with obesity and its comorbidities, demonstrating associations between nutrient intake and specific phyla.
وصف الملف: electronic resource
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4دورية أكاديمية
المؤلفون: Natália Yumi Noronha, Isabella Harumi Yonehara Noma, Rafael Fernandes Ferreira, Guilherme da Silva Rodrigues, Luzania dos Santos Martins, Lígia Moriguchi Watanabe, Marcela Augusta de Souza Pinhel, Isabelle Mello Schineider, Luísa Maria Diani, Daniela Carlos, Carla Barbosa Nonino
المصدر: Frontiers in Nutrition, Vol 11 (2024)
مصطلحات موضوعية: DNA methylation, Actinobacteria, vitamin C, immune response and nutrients, epigenetic, Nutrition. Foods and food supply, TX341-641
الوصف: IntroductionThere is an emerging body of evidence that vitamin C consumption can modulate microbiota abundance and can also impact DNA methylation in the host, and this could be a link between diet, microbiota, and immune response. The objective of this study was to evaluate common CpG sites associated with both vitamin C and microbiota phyla abundance.MethodsSix healthy women participated in this cohort study. They were divided into two groups, according to the amount of vitamin C they ingested. Ingestion was evaluated using the 24-h recall method. The Illumina 450 k BeadChip was used to evaluate DNA methylation. Singular value decomposition analyses were used to evaluate the principal components of this dataset. Associations were evaluated using the differentially methylated position function from the Champ package for R Studio.Results and discussionThe group with higher vitamin C (HVC) ingestion also had a higher relative abundance of Actinobacteria. There was a positive correlation between those variables (r = 0.84, p = 0.01). The HVC group also had higher granulocytes, and regarding DNA methylation, there were 207 CpG sites commonly related to vitamin C ingestion and the relative abundance of Actinobacteria. From these sites, there were 13 sites hypomethylated and 103 hypermethylated. The hypomethylated targets involved the respective processes: immune function, glucose homeostasis, and general cellular metabolism. The hypermethylated sites were also enriched in immune function-related processes, and interestingly, more immune responses against pathogens were detected. These findings contribute to understanding the interaction between nutrients, microbiota, DNA methylation, and the immune response.
وصف الملف: electronic resource
العلاقة: https://www.frontiersin.org/articles/10.3389/fnut.2024.1373499/fullTest; https://doaj.org/toc/2296-861XTest
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5دورية أكاديمية
المؤلفون: Guilherme da Silva Rodrigues, Natália Yumi Noronha, Isabella Harumi Yonehara Noma, João Gabriel Ribeiro de Lima, Andressa Crystine da Silva Sobrinho, Marcela Augusta de Souza Pinhel, Mariana Luciano de Almeida, Lígia Moriguchi Watanabe, Carla Barbosa Nonino, Carlos Roberto Bueno Júnior
المصدر: Experimental Gerontology, Vol 186, Iss , Pp 112362- (2024)
مصطلحات موضوعية: Epigenetic, Combined training, Women, Physical activity, Medicine, Biology (General), QH301-705.5
الوصف: Exercise training emerges as a key strategy in lifestyle modification, capable of reducing the risk of developing Alzheimer's disease (AD) due to risk factors such as age, family history, genetics and low level of education associated with AD. We aim to analyze the effect of a 14-week combined exercise training (CT) on the methylation of genes associated with AD in non-alzheimer's disease women. CT sessions lasted 60 min, occurring three times a week for 14 weeks. Forty non-Alzheimer's disease women aged 50 to 70 years (60.7 ± 4.1 years) with a mean height of 1.6 ± 0.1 m, mean weight of 73.12 ± 9.0 kg and a mean body mass index of 29.69 ± 3.5 kg/m2, underwent two physical assessments: pre and post the 14 weeks. DNA methylation assays utilized the EPIC Infinium Methylation BeadChip from Illumina. We observed that 14 weeks of CT led to reductions in systolic (p = 0.001) and diastolic (p = 0.017) blood pressure and improved motor skills post-intervention. Among 25 genes linked to AD, CT induced differentially methylated sites in 12 genes, predominantly showing hypomethylated sites (negative β values). Interestingly, despite hypomethylated sites, some genes exhibited hypermethylated sites (positive β values), such as ABCA7, BDNF, and WWOX. A 14-week CT regimen was adequate to induce differential methylation in 12 CE-related genes in healthy older women, alongside improvements in motor skills and blood pressure. In conclusion, this study suggest that combined training can be a strategy to improve physical fitness in older individuals, especially able to induce methylation alterations in genes sites related to development of AD. It is important to highlight that training should act as protective factor in older adults.
وصف الملف: electronic resource
العلاقة: http://www.sciencedirect.com/science/article/pii/S0531556524000044Test; https://doaj.org/toc/1873-6815Test
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6دورية أكاديمية
المؤلفون: Ligia Moriguchi Watanabe, Lisete Sousa, Francisco M. Couto, Natália Yumi Noronha, Marcela Augusta de Souza Pinhel, Gleyson Francisco da Silva Carvalho, Guilherme da Silva Rodrigues, Carlos Roberto Bueno Júnior, Leslie Domenici Kulikowski, Fernando Barbosa Júnior, Carla Barbosa Nonino
المصدر: Nutrients, Vol 16, Iss 11, p 1627 (2024)
مصطلحات موضوعية: selenium deficiency, GWAS, admixed population, Nutrition. Foods and food supply, TX341-641
الوصف: Blood selenium (Se) concentrations differ substantially by population and could be influenced by genetic variants, increasing Se deficiency-related diseases. We conducted a genome-wide association study (GWAS) to identify single nucleotide polymorphisms (SNPs) associated with serum Se deficiency in 382 adults with admixed ancestry. Genotyping arrays were combined to yield 90,937 SNPs. R packages were applied to quality control and imputation. We also performed the ancestral proportion analysis. The Search Tool for the Retrieval of Interacting Genes was used to interrogate known protein–protein interaction networks (PPIs). Our ancestral proportion analysis estimated 71% of the genome was from Caucasians, 22% was from Africans, and 8% was from East Asians. We identified the SNP rs1561573 in the TraB domain containing 2B (TRABD2B), rs425664 in MAF bZIP transcription factor (MAF), rs10444656 in spermatogenesis-associated 13 (SPATA13), and rs6592284 in heat shock protein nuclear import factor (HIKESHI) genes. The PPI analysis showed functional associations of Se deficiency, thyroid hormone metabolism, NRF2-ARE and the Wnt pathway, and heat stress. Our findings show evidence of a genetic association between Se deficiency and metabolic pathways indirectly linked to Se regulation, reinforcing the complex relationship between Se intake and the endogenous factors affecting the Se requirements for optimal health.
وصف الملف: electronic resource
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7دورية أكاديمية
المؤلفون: Amanda A Ribeiro, Lucas M Carvalho, Jhulia C.N.L. da Mota, Carla Barbosa Nonino, Bruno Gualano, Juan A.V Nunes, José Alfredo Martinez, Carolina F Nicoletti
المصدر: Lifestyle Genomics, Pp 1-1 (2024)
الوصف: Background: The pathoetiology of systemic lupus erythematosus (SLE) involves a multifactorial interaction consisting of various genetic, epigenetic and environmental factors. Considering epigenetic characteristics, notable alterations in DNA methylation, particularly hypomethylation in immune-related pathways, such as T cell receptor have been observed. In turn, these alterations are associated with the overexpression of genes related to autoimmunity and a loss of immunological self-tolerance. Furthermore, DNA hypomethylation levels in SLE may contribute to disease progression and also impact disease activity and clinical manifestations. Summary: It is well-established that nutritional epigenetics elucidates the role of nutrition and dietary factors on the interactions of metabolic systems with the molecules that bind to DNA, regulating gene expression. Specific nutritional interventions may reverse initial epigenetic patterns, thereby significantly impacting the chronic disease´s treatment and prognostic. In fact, dietary nutrients and bioactive food compounds may influence DNA methylation patterns by inhibiting enzymes related to DNA methylation reactions or by altering the availability of different substrates involved in DNA methylation process (e.g methyl-donor nutrients). Key Messages: The knowledge of how diet plays a role in changing DNA methylation patterns in SLE is in the early stages. While a few studies in the literature have assessed the effects of nutrient intake, supplementation, or treatment on DNA methylation levels and have demonstrated their relevance, further research is imperative to deepen our comprehension of the interactions between epigenetics and nutrients, which is vital for the development of novel precision nutrition approaches.
وصف الملف: electronic resource
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8دورية أكاديمية
المؤلفون: Tanu-udom Maneesing, Atchara Dawangpa, Pechngam Chaivanit, Sudjai Songsakul, Piyapong Prasertsri, Natália Yumi Noronha, Lígia Moriguchi Watanabe, Carla Barbosa Nonino, Busadee Pratumvinit, Chanachai Sae-Lee
المصدر: Frontiers in Nutrition, Vol 10 (2023)
مصطلحات موضوعية: portioned meal box, dietary counselling, type 2 diabetes mellitus, fasting blood glucose, HbA1c, Nutrition. Foods and food supply, TX341-641
الوصف: BackgroundThe impact of dietary factors on glycaemic control in type 2 diabetes mellitus (T2DM) is well established. However, the effectiveness of transforming portion control into a practical innovation for glycaemic control in T2DM has not yet been established for counselling in nutrition. The aim of this study was to compare the effect of general counselling in nutrition (GCN) and a portioned meal box (PMB) on fasting blood glucose, glycated haemoglobin (HbA1c) and body composition.MethodsA randomised, parallel intervention trial was conducted over 12 weeks, with GCN: carbohydrate portion control concept by using food exchange lists (n = 25) and PMB: portioned meal box was set by energy requirements (n = 25).ResultsBoth GCN and PMB demonstrated reductions in HbA1c levels at the 6th and 12th weeks compared to baseline. However, no significant difference in HbA1c was observed between GCN and PMB at either the 6th or 12th week. Using PMB at least four times a week significantly decreased HbA1c during the intervention period (p = 0.021 and p
وصف الملف: electronic resource
العلاقة: https://www.frontiersin.org/articles/10.3389/fnut.2023.1216753/fullTest; https://doaj.org/toc/2296-861XTest
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9دورية أكاديمية
المؤلفون: Guilherme da Silva Rodrigues, Natalia Yumi Noronha, João Gabriel Ribeiro de Lima, Isabela Harumi Yonehara Noma, Andressa Crystine da Silva Sobrinho, Luísa Maria Diani, Ana P. Pinto, Karine Pereira Rodrigues, Marcela Augusta de Souza Pinhel, Carla Barbosa Nonino, Lígia Moriguchi Watanabe, Carlos Roberto Bueno Júnior
المصدر: Epigenetics, Vol 19, Iss 1 (2024)
مصطلحات موضوعية: Physical exercise, epigenetics, women, DNA methylation, Genetics, QH426-470
الوصف: The mechanisms by which the ageing process is associated to an unhealthy lifestyle and how they play an essential role in the aetiology of systemic arterial hypertension have not yet been completely elucidated. Our objective is to investigate the influence of NOS3 polymorphisms [−786T > C and (Glu298Asp)] on systolic blood pressure (SBP) and diastolic blood pressure (DBP) response, differentially methylated regions (DMRs), and physical fitness of adult and older women after a 14-week combined training intervention. The combined training was carried out for 14 weeks, performed 3 times a week, totalling 180 minutes weekly. The genotyping experiment used Illumina Infinium Global Screening Array version 2.0 (GSA V2.0) and Illumina’s EPIC Infinium Methylation BeadChip. The participants were separated into SNP rs2070744 in TT (59.7 ± 6.2 years) and TC + CC (60.0 ± 5.2 years), and SNP rs17999 in GluGlu (58.8 ± 5.7 years) and GluAsp + AspAsp (61.6 ± 4.9 years). We observed an effect of time for variables BP, physical capacities, and cholesterol. DMRs related to SBP and DBP were identified for the rs2070744 and rs17999 groups pre- and decreased numbers of DMRs post-training. When we analysed the effect of exercise training in pre- and post-comparisons, the GluGlu SNP (rs17999) showed 10 DMRs, and after enrichment, we identified several biological biases. The combined training improved the SBP and DBP values of the participants regardless of the SNPs. In addition, exercise training affected DNA methylation differently between the groups of NOS3 polymorphisms.
وصف الملف: electronic resource
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10دورية أكاديمية
المؤلفون: Bruna Morais Faleiros de Paula, Marcela Augusta de Souza Pinhel, Carolina Ferreira Nicoletti, Carla Barbosa Nonino, Flávia Siqueira, Helio Vannucchi
المصدر: Clinical Nutrition Open Science, Vol 44, Iss , Pp 15-27 (2022)
مصطلحات موضوعية: Epigenetics, DNA Methylation, Pparγ, Foxo1, Folate supplementation, Energy metabolism, Nutrition. Foods and food supply, TX341-641
الوصف: Summary: Background & aims: Nutritional epigenetics involves essential mechanisms for proper embryonic development in mammals. Changes in this stage cause various metabolic disorders. Under healthy conditions, epigenetic processes and adequate homeostasis, especially energy metabolism, are balanced. However, interruption or imbalance of metabolic homeostasis, or both, leads to several pathologies, such as insulin resistance, cardiovascular diseases, and obesity. This study investigates the methylation and expression patterns of the peroxisome proliferator-activated receptor gamma (Pparγ) and forkhead Box 1 (Foxo1) genes in rat offspring submitted to folate-deficient or folate-supplemented diet. Methods: Groups of male and female Wistar rat offspring (n=40 per group, 3 groups) were fed folate-normal (control), a folate-deficient, or a folate-supplemented diet (2.0, 0.5, or 8.0 mg of folic acid/kg of feed, respectively) for 13 weeks. The diet was the same diet the mothers had received during pregnancy and lactation. Liver, white adipose tissue (WAT), and brown adipose tissue (BAT) samples were collected. Specific commercial kits were used to extract RNA, DNA, and protein from these samples. Gene expression, DNA methylation, and protein expression were analyzed by polymerase chain reaction (PCR), Pyrosequencing, and Western Blot, respectively. Results: In liver, the Pparγ and Foxo1 genes did not differ in terms of DNA methylation; however, Pparγ gene expression was higher (P=0.03) in the folate-supplemented diet group than in the other groups. Hepatic Pparγ protein expression was higher (P=0.00) in the folate-supplemented and folate-deficient diet groups than in the control diet group. Hepatic Foxo1 protein expression was lower (P=0.01) in the folate-supplemented diet group than in the other groups. In WAT, Foxo1 methylation was lower (P=0.00) in the folate-supplemented diet group than in the control diet group; Pparγ methylation was lower (P=0.02) in the folate-deficient diet group than in the control diet group; and Foxo1 gene expression was higher (P=0.01) in the folate-supplemented diet group than in the folate-deficient diet group. In BAT, the folate-deficient and folate-supplemented diet groups had lower Pparγ gene expression (P=0.00) and protein (P=0.00) expression than the control diet group. Conclusion: Depending on offspring sex and tissue, folate-supplemented diet generally modulates genes in a beneficial way because these genes are involved in energy metabolism and may participate in some pathways related to metabolic diseases.
وصف الملف: electronic resource
العلاقة: http://www.sciencedirect.com/science/article/pii/S2667268522000122Test; https://doaj.org/toc/2667-2685Test
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