المؤلفون: Adrián, José A., Bermúdez-Llusá, Geidy, Caramés, José M., Rodríguez-Parra, María J., Arango-Lasprilla, Juan C.

المصدر: American Journal of Speech-Language Pathology; Nov2023, Vol. 32, p2615-2629, 15p

مصطلحات موضوعية: STATISTICS, LANGUAGE disorders in old age, MEDICAL screening, SPEECH evaluation, DISCRIMINANT analysis, AGING, DESCRIPTIVE statistics, DATA analysis, DATA analysis software, SENSITIVITY & specificity (Statistics)

مصطلحات جغرافية: SPAIN

مستخلص: Purpose: The NeuroBel is a short test that can detect cognitive decline using language tasks. This study replicated previous research using larger clinical samples from three Spanish-speaking countries. Method: Eight tasks were used to analyze verbal language functioning using a psycholinguistic approach. A total of 232 elderly, monolingual Spanish speakers from Spain, Cuba, and Colombia participated in this study. Of these, 76 had Alzheimer's disease (AD) in the initial phase, 75 had mild cognitive impairment (MCI), and 81 did not exhibit cognitive impairment (healthy controls). Results: Significant differences were observed among the three clinical groups. The participants with AD and the participants with MCI had significantly lower NeuroBel scores than the control group on most of the tasks. However, repetition (in AD vs. MCI) and auditory lexical decision (in MCI vs. control) tasks were not significant in Tukey's post hoc tests. Discriminant analysis showed that 80.6% of the participants were correctly classified into the original groups and revealed the tasks that were the best and worst for differentiating among groups. The receiver-operating characteristic curves showed high sensitivity for AD and MCI. The area under the curve was .97 in the contrast of AD versus MCI + controls, .96 in the determination of overall cognitive decline (AD + MCI vs. controls), and .93 in the contrast of MCI and control groups. Conclusion: This study confirmed that the NeuroBel is a suitable test for detecting cognitive decline based on language impairment in Spanish-speaking elderly people. [ABSTRACT FROM AUTHOR]

: Copyright of American Journal of Speech-Language Pathology is the property of American Speech-Language-Hearing Association and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Lipinski, Michal, Muñoz-Viana, Rafael, Blanco, Beatriz del, Marquez-Galera, Angel, Medrano-Relinque, Juan, Caramés, José M., Szczepankiewicz, Andrzej A., Fernandez-Albert, Jordi, Navarrón, Carmen M., Olivares, Román, Wilczyński, Grzegorz M., Canals Gamoneda, Santiago, López-Atalaya, José P., Barco, Ángel

المساهمون: Ministerio de Ciencia, Innovación y Universidades (España), Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España), European Commission, Generalitat Valenciana, Polish Academy of Sciences, Ministerio de Educación, Cultura y Deporte (España), Lipinski, Michal, Muñoz-Viana, Rafael, Szczepankiewicz, Andrzej A., Navarrón, Carmen M., Canals Gamoneda, Santiago, Barco, Ángel

الوصف: The lysine acetyltransferases type 3 (KAT3) family members CBP and p300 are important transcriptional co-activators, but their specific functions in adult post-mitotic neurons remain unclear. Here, we show that the combined elimination of both proteins in forebrain excitatory neurons of adult mice resulted in a rapidly progressing neurological phenotype associated with severe ataxia, dendritic retraction and reduced electrical activity. At the molecular level, we observed the downregulation of neuronal genes, as well as decreased H3K27 acetylation and pro-neural transcription factor binding at the promoters and enhancers of canonical neuronal genes. The combined deletion of CBP and p300 in hippocampal neurons resulted in the rapid loss of neuronal molecular identity without de- or transdifferentiation. Restoring CBP expression or lysine acetylation rescued neuronal-specific transcription in cultured neurons. Together, these experiments show that KAT3 proteins maintain the excitatory neuron identity through the regulation of histone acetylation at cell type-specific promoter and enhancer regions. ; M.L. is recipient of a Santiago Grisolia fellowship given by the Generalitat Valenciana, J.M.C. is recipient of a fellowship from the Spanish Ministry of Education, Culture and Sport (MECD), J.F.-A. and C.M.N. are recipients of fellowships from the Spanish Ministry of Science and Innovation (MICINN). The ultrastructure research was supported by the Polish National Science Center Grant UMO-2014/15/N/NZ3/04468 and by the European Regional Development Fund POIG 01.01.02-00-008/08. J.P.L.-A. research is supported by Grants RYC-2015-18056 and RTI2018-102260-B-I00 from MICINN co-financed by ERDF. A.B. research is supported by Grants SAF2017-87928-R, PCIN-2015-192-C02-01, and SEV-2017-0723 from MICINN co-financed by ERDF, PROMETEO/2016/026 from the Generalitat Valenciana, and RGP0039/2017 from the Human Frontiers Science Program Organization (HFSPO). The Instituto de Neurociencias is a “Centre of Excellence Severo Ochoa”. ...

العلاقة: #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC-2015-18056; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-102260-B-I00; RTI2018-102260-B-I00/AEI/10.13039/501100011033; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/SAF2017-87928-R; SAF2017-87928-R/AEI/10.13039/501100011033; info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/PCIN-2015-192-C02-01; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/SEV-2017-0723; SEV-2017-0723/AEI/10.13039/501100011033; Publisher's version; https://doi.org/10.1038/s41467-020-16246-0Test; Sí; Nature Communications 11: 2588 (2020); http://hdl.handle.net/10261/217878Test; http://dx.doi.org/10.13039/501100000780Test; http://dx.doi.org/10.13039/501100011033Test; http://dx.doi.org/10.13039/501100003359Test; http://dx.doi.org/10.13039/501100003329Test; http://dx.doi.org/10.13039/501100003176Test

الإتاحة: https://doi.org/10.1038/s41467-020-16246-0Test
https://doi.org/10.13039/501100000780Test
https://doi.org/10.13039/501100011033Test
https://doi.org/10.13039/501100003359Test
https://doi.org/10.13039/501100003329Test
https://doi.org/10.13039/501100003176Test
http://hdl.handle.net/10261/217878Test

المؤلفون: Quiñones, Darío R., Fernández-Mollá, Luis Miguel, Pacheco-Torres, Jesús, Caramés, José M., Canals, Santiago, Moratal, David

المساهمون: Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica, European Commission, European Regional Development Fund, Universitat Politècnica de València, Ministerio de Economía y Competitividad

مصطلحات موضوعية: Anesthetic, Arduino, BOLD, FMRI, Mice, PID, TECNOLOGIA ELECTRONICA

الوصف: [EN] Introduction: Functional magnetic resonance imaging (fMRI) is one of the most highly regarded techniques in the neuroimaging field. This technique is based on vascular responses to neuronal activation and is extensively used in clinical and animal research studies. In preclinical settings, fMRI is usually applied to anesthetized animals. However, anesthetics cause alterations, e.g. hypothermia, in the physiology of the animals and this has the potential to disrupt fMRI signals. The current temperature control method involves a technician, as well as monitoring the acquisition MRI sequences, also controlling the temperature of the animal; this is inefficient. Methods: In order to avoid hypothermia in anesthetized rodents an Open-Source automatic temperature control device is presented. It takes signals from an intrarectal temperature sensor, as well as signals from a thermal bath, which warms up the body of the animal under study, in order to determine the mathematical model of the thermal response of the animal. Results: A Proportional-Integral-Derivative (PID) algorithm, which was discretized in an Arduino micro-controller, was developed to automatically keep stable the body temperature of the animal under study. The PID algorithm has been shown to be accurate in preserving the body temperature of the animal. Conclusion: This work presents the TherMouseDuino. It is an Open-Source automatic temperature control system and reduces temperature fluctuations, thus providing robust conditions in which to perform fMRI experiments. Furthermore, our device frees up the technician to focus solely on monitoring the MRI sequences. ; This work was supported in part by the Spanish Ministerio de Economia y Competitividad (MINECO) and FEDER funds under grants BFU2015-64380-C2-2-R (D.M.) and BFU2015-64380-C2-1-R and EU Horizon 2020 Program 668863-SyBil-AA grant (S.C.). S.C. acknowledges financial support from the Spanish State Research Agency, through the "Severo Ochoa" Programme for Centres of Excellence in R&D (ref. ...

العلاقة: Magnetic Resonance Imaging; info:eu-repo/grantAgreement/EC/H2020/668863/EU/Systems Biology of Alcohol Addiction: Modeling and validating disease state networks in human and animal brains for understanding pathophysiolgy, predicting outcomes and improving therapy/; info:eu-repo/grantAgreement/MINECO//BFU2015-64380-C2-1-R/ES/TRATAR LA ENFERMEDAD RESINTONIZANDO LA DINAMICA DE LAS REDES CEREBRALES/; info:eu-repo/grantAgreement/MINECO//SEV-2013-0317/ES/-/; info:eu-repo/grantAgreement/MINECO//BFU2015-64380-C2-2-R/ES/ANALISIS DE TEXTURAS EN IMAGEN CEREBRAL MULTIMODAL POR RESONANCIA MAGNETICA PARA UNA DETECCION TEMPRANA DE ALTERACIONES EN LA RED Y BIOMARCADORES DE ENFERMEDAD/; https://doi.org/10.1016/j.mri.2019.01.009Test; urn:issn:0730-725X; http://hdl.handle.net/10251/159217Test

الإتاحة: https://doi.org/10.1016/j.mri.2019.01.009Test
http://hdl.handle.net/10251/159217Test

المؤلفون: Cabo, Fernando, Caramés, José M., Almaraz, Julián, Espinet, Alfredo

المصدر: Psicológica Journal ; volume 39, issue 1, page 25-40 ; ISSN 1576-8597

الوصف: Extinction of the A↔X association after blocked preexposure to AX-BX was studied in two experiments. In Experiment 1, two groups of rats received long (14 trials) or short (4 trials) blocked preexposure to AX-BX and subsequent conditioning of X. The results showed that the AX association was equally preserved after long and short preexposure. Experiment 2 studied the effect of blocked preexposure to 0, 1 or 2 ruptures of the AX association on extinction. In this experiment a “rupture” is produced when, in subsequent blocks, one element of the original compound is presented in compound with a different element. A significant extinction was observed only when the AX association was broken twice

الإتاحة: https://doi.org/10.2478/psicolj-2018-0002Test
https://content.sciendo.com/view/journals/psicolj/39/1/article-p25.xmlTest

المؤلفون: García-Hernández, Raquel, Caramés, José M., Pérez-Montoyo, Elena, Canals Gamoneda, Santiago

المساهمون: Ministerio de Economía y Competitividad (España), European Commission, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España)

الوصف: Trabajo presentado al 12th Federation of European Neuroscience Societies (FENS): Virtual Forum of NeuroScience, celebrado del 11 al 15 de julio de 2020. ; Granule cells in the dentate gyrus (DGgc), a brain region important for spatial learning, are part of the engrams formed when an animal explores a new context. Previous work showed that modulation of DGgc activity by perisomatic inhibition bidirectionally regulates memory encoding. Whether this result is due to a differential recruitment of experience-relevant neuronal assemblies or the functional connectivity between them, is not yet known. We combined pharmacogenetic tools (DREADDs) to increase or decrease the activity of parvalbumin (PV)-interneurons in DG while mice encoded spatial information in the Novel Object Location task (NOL). Sixty min after memory encoding in the NOL task animals were sacrificed and their brains processed and quantified for c-Fos staining. Exploration in the NOL task induced a robust increase in the number of c-Fos+ cells across hippocampal subfields. However, the number of c-Fos+ cells, both in the hippocampus and extra-hippocampal structures like the medial prefrontal cortex (mPFC) and the nucleus accumbens, was constant regardless of the inhibitory tone in the DG. Only a moderate increase in c-Fos intensity per cell in DGgc was found in the PV-cell inhibition group. In contrast, we found a significant increase in the correlation between the number of c-Fos+ cells in all quantified neuronal assemblies during PV-inhibition, and a decrease during activation. Together, these data reveal a critical regulatory role of perisomatic inhibition in the dentate gyrus in binding experience-relevant neuronal assemblies in memory. ; This work was supported by the Spanish Ministerio de Economía y Competitividad (MINECO) and FEDER funds under the grant PGC2018-101055-B-100). ; Peer reviewed

العلاقة: #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-101055-B-100; PGC2018-101055-B-100/AEI/10.13039/501100011033; Publisher's version; Sí; 12th FENS: Virtual Forum of NeuroScience (2020); http://hdl.handle.net/10261/221839Test; http://dx.doi.org/10.13039/501100003329Test; http://dx.doi.org/10.13039/501100011033Test; http://dx.doi.org/10.13039/501100000780Test

الإتاحة: https://doi.org/10.13039/501100003329Test
https://doi.org/10.13039/501100011033Test
https://doi.org/10.13039/501100000780Test
http://hdl.handle.net/10261/221839Test

المؤلفون: Espinet, Alfredo, Caramés, José M., Cabo, Fernando

المصدر: Behavioural Processes ; volume 120, page 94-100 ; ISSN 0376-6357

مصطلحات موضوعية: Behavioral Neuroscience, Animal Science and Zoology, General Medicine

الإتاحة: https://doi.org/10.1016/j.beproc.2015.09.003Test
https://api.elsevier.com/content/article/PII:S0376635715300334?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0376635715300334?httpAccept=text/plainTest

المؤلفون: Suárez, Luz M., Solís, Oscar, Caramés, Jose M., Taravini, Irene R., Solís, Jose M., Murer, Mario G., Moratalla, Rosario

المصدر: Biological Psychiatry ; volume 75, issue 9, page 711-722 ; ISSN 0006-3223

مصطلحات موضوعية: Biological Psychiatry

الإتاحة: https://doi.org/10.1016/j.biopsych.2013.05.006Test
https://api.elsevier.com/content/article/PII:S0006322313004162?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0006322313004162?httpAccept=text/plainTest

المؤلفون: Espinet, Alfredo, Caramés, José M., Chamizo, Victoria D.

المساهمون: Ministerio de Educación y Ciencia

المصدر: Behavioural Processes ; volume 88, issue 2, page 94-100 ; ISSN 0376-6357

مصطلحات موضوعية: Behavioral Neuroscience, Animal Science and Zoology, General Medicine

الإتاحة: https://doi.org/10.1016/j.beproc.2011.08.001Test
https://api.elsevier.com/content/article/PII:S0376635711001549?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0376635711001549?httpAccept=text/plainTest

المؤلفون: Aguilar, Raúl, Caramés, José M., Espinet, Alfredo

المساهمون: Spanish Ministerio de Educacion y Ciencia, Universidad de Málaga

المصدر: Journal of Comparative Psychology ; volume 123, issue 4, page 347-356 ; ISSN 1939-2087 0735-7036

الإتاحة: https://doi.org/10.1037/a0016437Test
http://psycnet.apa.org/journals/com/123/4/347.pdfTest

المؤلفون: Suárez, Luz M., Solís, Oscar, Caramés, Jose M., Taravini, Irene Rita Eloisa, Solís, Jose M., Murer, Mario Gustavo, Moratalla, Rosario

مصطلحات موضوعية: Behavioral Sensitization, Dyskinesia, L-Dopa, Medium Spiny Neuron, Parkinson'S Disease, Striatum, Three-Dimensional Neuronal Recontruction, https://purl.org/becyt/ford/3.1Test, https://purl.org/becyt/ford/3Test

الوصف: Background: L-3,4-dihydroxyphenylalanine (L-DOPA)–induced dyskinesia is an incapacitating complication of L-DOPA therapy that affects most patients with Parkinson’s disease. Previous work indicating that molecular sensitization to dopamine receptor D1 (D1R) stimulation is involved in dyskinesias prompted us to perform electrophysiological recordings of striatal projection “medium spiny neurons” (MSN). Moreover, because enhanced D1R signaling in drug abuse induces changes in spine density in striatum, we investigated whether the dyskinesia is related to morphological changes in MSNs. Methods: Wild-type and bacterial artificial chromosome transgenic mice (D1R-tomato and D2R–green fluorescent protein) mice were lesioned with 6-hydroxydopamine and subsequently treated with L-DOPA to induce dyskinesia. Functional, molecular, and structural changes were assessed in corticostriatal slices. Individual MSNs injected with Lucifer-Yellow were detected by immunohistochemistry for three-dimensional reconstructions with Neurolucida software. Intracellular current-clamp recordings with high-resistance micropipettes were used to characterize electrophysiological parameters. Results: Both D1R-MSNs and D2R-MSNs showed diminished spine density in totally denervated striatal regions in parkinsonian mice. Chronic L-DOPA treatment, which induced dyskinesia and aberrant FosB expression, restored spine density in D2R-MSNs but not in D1R-MSNs. In basal conditions, MSNs are more excitable in parkinsonian than in sham mice, and excitability decreases toward normal values after L-DOPA treatment. Despite this normalization of basal excitability, in dyskinetic mice, the selective D1R agonist SKF38393 increased the number of evoked action potentials in MSNs, compared with sham animals. Conclusions: Chronic L-DOPA induces abnormal spine re-growth exclusively in D2R-MSNs and robust supersensitization to D1R-activated excitability in denervated striatal MSNs. These changes might constitute the anatomical and electrophysiological substrates of ...

وصف الملف: application/pdf

العلاقة: info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0006322313004162Test; http://hdl.handle.net/11336/13605Test; Suárez, Luz M.; Solís, Oscar; Caramés, Jose M.; Taravini, Irene Rita Eloisa; Solís, Jose M.; et al.; L-DOPA treatment selectively restores spine density in dopamine receptor d2-expressing projection neurons in dyskinetic mice; Elsevier; Biological Psychiatry; 75; 9; 5-2014; 711-722

الإتاحة: https://doi.org/10.1016/j.biopsych.2013.05.006Test
http://hdl.handle.net/11336/13605Test