المؤلفون: Iglesias Gonzalez, Ana Belen, Koning, Harmen Kornelis, 1991, Tuz-Sasik, Melek Umay, van Osselen, Ilse, Manuel, Remy, Boije, Henrik

المصدر: Developmental Biology. 508:77-87

مصطلحات موضوعية: dmrt3a, Spinal cord, Evx2, Calcium binding protein, Locomotor network

الوصف: Calcium binding proteins are essential for neural development and cellular activity. Calretinin, encoded by calb2a and calb2b, plays a role during early zebrafish development and has been proposed as a marker for distinct neuronal populations within the locomotor network. We generated a calb2b:hs:eGFP transgenic reporter line to characterize calretinin expressing cells in the developing spinal cord and describe morphological and behavioral defects in calretinin knock-down larvae. eGFP was detected in primary and secondary motor neurons, as well as in dI6 and V0v interneurons. Knock-down of calretinin lead to disturbed development of motor neurons and dI6 interneurons, revealing a crucial role during early development of the locomotor network. Primary motor neurons showed delayed axon outgrowth and the distinct inhibitory CoLo neurons, originating from the dI6 lineage, were absent. These observations explain the locomotor defects we observed in calretinin knock-down animals where the velocity, acceleration and coordination were affected during escapes. Altogether, our analysis suggests an essential role for calretinin during the development of the circuits regulating escape responses and fast movements within the locomotor network.

وصف الملف: electronic

الوصول الحر: https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-528186Test
https://doi.org/10.1016/j.ydbio.2024.01.001Test
https://uu.diva-portal.org/smash/get/diva2:1858728/FULLTEXT01.pdfTest

المؤلفون: Shukla, Sankalp, Chen, Wei, Rao, Shanlin, Yang, Serim, Ou, Chenxi, Larsen, Kevin, Hummer, Gerhard, Hanson, Phyllis, Hurley, James

المصدر: Proceedings of the National Academy of Sciences of the United States of America. 121(9)

مصطلحات موضوعية: ESCRT, calcium-binding protein, lysosome, membrane repair, reconstitution, Membranes, Cell Physiological Phenomena, Intracellular Membranes, Cell Division, Endosomal Sorting Complexes Required for Transport

الوصف: Apoptosis linked Gene-2 (ALG-2) is a multifunctional intracellular Ca2+ sensor and the archetypal member of the penta-EF hand protein family. ALG-2 functions in the repair of damage to both the plasma and lysosome membranes and in COPII-dependent budding at endoplasmic reticulum exit sites (ERES). In the presence of Ca2+, ALG-2 binds to ESCRT-I and ALIX in membrane repair and to SEC31A at ERES. ALG-2 also binds directly to acidic membranes in the presence of Ca2+ by a combination of electrostatic and hydrophobic interactions. By combining giant unilamellar vesicle-based experiments and molecular dynamics simulations, we show that charge-reversed mutants of ALG-2 at these locations disrupt membrane recruitment. ALG-2 membrane binding mutants have reduced or abrogated ERES localization in response to Thapsigargin-induced Ca2+ release but still localize to lysosomes following lysosomal Ca2+ release. In vitro reconstitution shows that the ALG-2 membrane-binding defect can be rescued by binding to ESCRT-I. These data thus reveal the nature of direct Ca2+-dependent membrane binding and its interplay with Ca2+-dependent protein binding in the cellular functions of ALG-2.

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/02g5j8hxTest

المؤلفون: XUAN SHI, YUFEN WANG, CHENYU LI, WANGSHU FU, XINYUE ZHANG, AIXIA GONG

المصدر: Biocell; 2024, Vol. 48 Issue 5, p835-845, 11p

مصطلحات موضوعية: COLORECTAL cancer, APOPTOSIS, CALCIUM-binding protein gene expression, CELL survival, ISOTHIOCYANATES

مستخلص: The incidence of colorectal cancer (CRC) has been increasing in recent years. Thus, the discovery of factors that can assist in alleviating CRC is urgently warranted. Methods: To identify a potential factor involved in the development of CRC, we screened the upregulated genes in tumor tissues through four datasets from an online database. The expression of reticulocalbin 1 (RCN1), a Ca²⁺-binding protein, was upregulated in the four datasets. Based on loss-offunction experiments, the effect of RCN1 on cell viability was assessed by Cell Counting Kit-8 (CCK-8) assay. The regulatory effect of RCN1 on apoptosis was evaluated through Annexin V-fluorescein 5-isothiocyanate (FITC)/propidium iodide (PI) staining assay and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay in RKO and SW480 cells. Activation of endoplasmic reticulum (ER) stress signaling pathways was confirmed by estimating the phosphorylation and expression of PRKR-like ER kinase (PERK), inositol-requiring kinase-1 (IRE1), transcription factor 6 (ACT6), and CCAAT/enhancer-binding protein-homologous protein (CHOP). The intracellular Ca²⁺ homeostasis regulated by RCN1 was determined through the detection of Ca²⁺ concentration and mitochondrial membrane potential (MMP) measurement. Moreover, whether inositol 1,4,5-trisphosphate receptor type 1 (IP3R1) was involved in the regulation of RCN1 in CRC was verified through the depletion of IP3R1 in RKO cells. Results: Knockdown of RCN1 reduced cell viability and facilitated apoptosis in RKO and SW480 cells. Phosphorylation of PERK and IRE1, activation of ATF6, and upregulation of CHOP were induced by the absence of RCN1, suggesting that the unfolded protein response (UPR) was activated in CRC cells. The concentration of Ca²⁺ in mitochondria was increased after RCN1 depletion, followed by reduction in the MMP and release of cytochrome c from mitochondria to the cytoplasm in RKO and SW480 cells. Moreover, it was demonstrated that IP3R1 mediates the effect of RCN1 on apoptosis induced by ER stress in CRC cells. The downregulation of IP3R1 restored the RCN1 loss-induced apoptosis and the increased Ca²⁺ concentration. Conclusion: Taken together, our results confirmed that silencing of RCN1 disrupted intracellular Ca²⁺ homeostasis and promoted cell apoptosis caused by TG-induced ER stress by regulating IP3R1 and activating the UPR signaling pathways. [ABSTRACT FROM AUTHOR]

: Copyright of Biocell is the property of Tech Science Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Asmaa Sabbah, Mohamed Elsayed, Hanan Helmy, Riham Hamdy Mostafa, Ghada Hatem

المصدر: The Egyptian Journal of Neurology, Psychiatry and Neurosurgery, Vol 60, Iss 1, Pp 1-9 (2024)

مصطلحات موضوعية: Biomarkers, C-reactive protein, Matrix metalloproteinase 9, S100 calcium-binding protein B, Brain natriuretic peptide, d-Dimer, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571

الوصف: Abstract Background Stroke is one of the most frequent causes of mortality and disability, blood protein biomarkers are used to determine patients at high risk for a severe illness and to estimate the outcome. This study aimed to detect the relation between serum levels of C-reactive protein, matrix metalloproteinase 9, S100 calcium-binding protein B, brain natriuretic peptide, D-dimer and stroke severity and outcome in acute ischemic stroke patients. Results One hundred eighty-six patients with acute ischemic stroke participated and were subjected to complete general, neurological examination, assessment of stroke severity clinically and radiologically using National Institute of Health Stroke Scale (NIHSS), and Alberta Stroke Program Early CT (ASPECT) score and assessment of functional outcome using (modified Rankin Scale). C-reactive protein, matrix metalloproteinase 9, S100 calcium-binding protein B, brain natriuretic peptide and D-dimer were assessed. Higher C-reactive protein was found in patients with ASPECT score ≤ 7 and in patients with cerebral edema, seizures and was positively correlated with stroke severity according to NIHSS and modified Rankin Scale. C-reactive protein serum level at onset was negatively correlated with NIHSS at onset and is a significant predictor for mortality. D-dimer was negatively correlated with NIHSS. S100 calcium-binding protein B was significantly elevated in patients who developed hemorrhagic transformation. Conclusions Serum C-reactive protein level can be used as a predictor for mortality and higher S100 calcium-binding protein B was detected in patients with hemorrhagic transformation.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1687-8329Test

الوصول الحر: https://doaj.org/article/14c64d5f87f843e3ab71a95eaf49bad4Test

المؤلفون: 杨会杰1，赵倩1，杜洁1，吕晓飞1，彭莹娟1，齐林

المصدر: Zhongguo cuzhong zazhi, Vol 18, Iss 11, Pp 1268-1276 (2023)

مصطلحات موضوعية: 桥接治疗, 脑梗死, 临床疗效, 神经损伤, 神经元特异性烯醇化酶, s100钙结合蛋白b, 胶质纤维酸性蛋白, bridging therapy, cerebral infarction, clinical effect, nerve injury, neuron-specific enolase, s100 calcium-binding protein b, glial fibrillary acidic protein, Neurology. Diseases of the nervous system, RC346-429

الوصف: 目的 探讨桥接治疗对前循环大血管闭塞急性脑梗死患者血清神经损伤相关生化标志物的影响以及临床疗效，为急性脑梗死治疗方式的选择提供参考依据。 方法 回顾性分析2018年10月—2020年6月前循环大血管闭塞的急性脑梗死患者的病历资料，根据是否采取桥接治疗分为对照组（静脉溶栓组）和观察组（桥接治疗组）。统计两组在治疗后24 h、14 d的治疗有效（NIHSS评分下降≥4分或NIHSS评分0分）率，治疗后30 d、90 d及180 d的良好预后 （mRS评分≤2分）率及日常生活能力自理（Barthel指数＞60分）率，住院期间脑出血发生率，治疗前以及治疗后3 d、7 d及14 d神经元特异性烯醇化酶（neuron-specific enolase，NSE）、S100钙结合蛋白B（S100 calcium-binding protein B，S100B）及胶质纤维酸性蛋白（glial fibrillary acidic protein，GFAP）水平。采用基于混合效应的logistic回归模型、线性混合效应模型进行预后指标的统计分析。 结果 多因素分析结果显示：观察组的治疗有效率是对照组的3.35倍（OR 3.35，95%CI 1.10～10.13，P=0.041）；观察组的良好预后率是对照组的4.12倍（OR 4.12，95%CI 1.14～14.82，P=0.035）；观察组的日常生活能力自理率与对照组相当（OR 1.47，95%CI 0.28～7.68，P=0.648）；观察组与对照组脑出血发生率差异无统计学意义（7.4% vs. 8.7%，P=1.000）；观察组NSE、S100B、GFAP水平低于对照组，差异具有统计学意义（P60) rate of normal living ability between two groups at 30 d, 90 d and 180 d after treatment, the incidence of intracerebral hemorrhage during hospitalization, and the related biochemical markers of serum nerve injury [neuron-specific enolase (NSE), S100 calcium-binding protein B (S100B) and glial fibrillary acidic protein (GFAP)] before and at 3 d, 7 d and 14 d after treatment were also analyzed. Logistic regression model based on mixed effects and linear mixed effects model were used for statistical analysis of prognostic indicators. Results Multivariate analysis showed that the treatment effective rate of the observation group was 3.35 times higher than that of the control group (OR 3.35, 95%CI 1.10-10.13, P=0.041). The good prognosis rate of the observation group may be 4.12 times higher than that of the control group (OR 4.12, 95%CI 1.14-14.82, P=0.035). The self-care rate of normal living ability in the observation group was similar to that in the control group (OR 1.47, 95%CI 0.28-7.68, P=0.648). There was no significant difference in the incidence of intracerebral hemorrhage between the observation group and the control group (7.4% vs. 8.7%, P=1.000). The levels of NSE, S100B and GFAP in the observation group were lower than those in the control group, and the differences were statistically significant (P

وصف الملف: electronic resource

العلاقة: http://www.chinastroke.org.cn/CN/article/openArticlePDF.jsp?id=3919Test; https://doaj.org/toc/1673-5765Test

الوصول الحر: https://doaj.org/article/40c1d227aa464358b4273c009647ed92Test

المؤلفون: Hamed Zarei, Shayan Roshdi Dizaji, Amirmohammad Toloui, Mahmoud Yousefifard, Alireza Esmaeili

المصدر: Archives of Academic Emergency Medicine, Vol 12, Iss 1 (2024)

مصطلحات موضوعية: Brain Injuries, Traumatic, Brain Concussion, Brain Contusion, Brain Hemorrhage, Traumatic, S100 Calcium Binding Protein Beta Subunit, S100 Proteins, Medical emergencies. Critical care. Intensive care. First aid, RC86-88.9

الوصف: Introduction: Traumatic brain injury (TBI) represents a significant global health burden. This systematic review delves into the comparison of S100B and Neuron-Specific Enolase (NSE) regarding their diagnostic and prognostic accuracy in TBI within the adult population. Methods: Conducted on October 21, 2023, the search identified 24 studies encompassing 6454 adult patients. QUADAS-2 and QUAPAS tools were employed to assess the risk of bias. The analyses aimed to evaluate the diagnostic and prognostic performance of S100B and NSE based on sensitivity, specificity, and area under the curve (AUC). The outcomes were detecting intracranial injury, mortality, and unfavorable outcome. Results: Pooled data analysis tended towards favoring S100B for diagnostic and prognostic purposes. S100B exhibited a diagnostic AUC of 0.74 (95% confidence interval (CI): 0.70-0.78), sensitivity of 80% (95% CI: 63%-90%), and specificity of 59% (95% CI: 45%-72%), outperforming NSE with an AUC of 0.66 (95% CI: 0.61–0.70), sensitivity of 74% (95% CI: 53%-88%), and specificity of 46% (95% CI: 24%-69%). Notably, both biomarkers demonstrated enhanced diagnostic value when blood samples were collected within 12 hours post-injury. The analyses also revealed the excellent diagnostic ability of S100B with a sensitivity of 99% (95% CI: 4%-100%) and a specificity of 76% (95% CI: 51%-91%) in mild TBI patients (AUC = 0.89 [0.86–0.91]). In predicting mortality, S100B showed a sensitivity of 90% (95% CI: 65%-98%) and specificity of 61% (95% CI: 39%-79%), slightly surpassing NSE's performance with a sensitivity of 88% (95% CI: 76%-95%) and specificity of 56% (95% CI: 47%-65%). For predicting unfavorable outcomes, S100B exhibited a sensitivity of 83% (95% CI: 74%-90%) and specificity of 51% (95% CI: 30%-72%), while NSE had a sensitivity of 80% (95% CI: 64%-90%) and specificity of 59% (95% CI: 46%-71%). Conclusion: Although neither biomarker has shown promising diagnostic performance in detecting abnormal computed tomography (CT) findings, they have displayed acceptable outcome prediction capabilities, particularly with regard to mortality.

وصف الملف: electronic resource

العلاقة: https://journals.sbmu.ac.ir/aaem/index.php/AAEM/article/view/2222Test; https://doaj.org/toc/2645-4904Test

الوصول الحر: https://doaj.org/article/70403aff62b7476bba46a06c5fd0e2b7Test

المؤلفون: Régis Nouvian

المصدر: eLife, Vol 13 (2024)

مصطلحات موضوعية: hearing, hair cells, calcium channel, calcium binding protein, glutamate, Medicine, Science, Biology (General), QH301-705.5

الوصف: Two calcium-binding proteins, CaBP1 and CaBP2, cooperate to keep calcium channels in the hair cells of the inner ear open.

وصف الملف: electronic resource

العلاقة: https://elifesciences.org/articles/96139Test; https://doaj.org/toc/2050-084XTest

الوصول الحر: https://doaj.org/article/10037ab8c9df440288b126ac28ebd16dTest

المؤلفون: Karaer, D., Durak, T., Karaer, K.

مصطلحات موضوعية: CCBE1 protein, human, calcium binding protein, tumor suppressor protein, case report, craniofacial malformation, intestine lymphangiectasia, lymphedema, mutation, Calcium-Binding Proteins, Craniofacial Abnormalities, Hennekam lymphangiectasia lymphedema syndrome, Humans, Lymphangiectasis, Intestinal, Tumor Suppressor Proteins

الوصف: [No abstract available]

العلاقة: Clinical Dysmorphology; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://hdl.handle.net/11499/56860Test; https://doi.org/10.1097/MCD.0000000000000488Test; 33; 87; 89; 2-s2.0-85186840507

الإتاحة: https://doi.org/10.1097/MCD.0000000000000488Test
https://hdl.handle.net/11499/56860Test

المؤلفون: Monroe, Derek C, Thomas, Elizabeth A, Cecchi, Nicholas J, Granger, Douglas A, Hicks, James W, Small, Steven L

المصدر: Scientific Reports. 12(1)

مصطلحات موضوعية: Biomedical and Clinical Sciences, Commercial Services, Commerce, Management, Tourism and Services, Brain Disorders, Physical Injury - Accidents and Adverse Effects, Clinical Research, Traumatic Head and Spine Injury, Neurosciences, Traumatic Brain Injury (TBI), Athletes, Biomarkers, Brain Concussion, Female, Humans, Intermediate Filaments, Male, S100 Calcium Binding Protein beta Subunit, Water Sports

الوصف: Blood-based biomarkers of brain injury may be useful for monitoring brain health in athletes at risk for concussions. Two putative biomarkers of sport-related concussion, neurofilament light (NfL), an axonal structural protein, and S100 calcium-binding protein beta (S100B), an astrocyte-derived protein, were measured in saliva, a biofluid which can be sampled in an athletic setting without the risks and burdens associated with blood sampled by venipuncture. Samples were collected from men's and women's collegiate water polo players (n = 65) before and after a competitive tournament. Head impacts were measured using sensors previously evaluated for use in water polo, and video recordings were independently reviewed for the purpose of validating impacts recorded by the sensors. Athletes sustained a total of 107 head impacts, all of which were asymptomatic (i.e., no athlete was diagnosed with a concussion or more serious). Post-tournament salivary NfL was directly associated with head impact frequency (RR = 1.151, p = 0.025) and cumulative head impact magnitude (RR = 1.008, p = 0.014), while controlling for baseline salivary NfL. Change in S100B was not associated with head impact exposure (RR  0.483). These patterns suggest that repeated head impacts may cause axonal injury, even in asymptomatic athletes.

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/6rj7m2w2Test

المؤلفون: Shu-Hui Wang, Yan-Jie Xia, Jing Yu, Chun-Yan He, Jie-Ru Han, Ji-Xiang Bai

المصدر: Journal of Investigative Surgery, Vol 36, Iss 1 (2023)

مصطلحات موضوعية: renal cell carcinoma, s100 calcium-binding protein a8, proliferation, apoptosis, nf-κb, Surgery, RD1-811

الوصف: Background Renal cell carcinoma (RCC), arising from the renal tubular epithelium, is one of the most common types of genitourinary malignancies. Based on the Gene Expression Omnibus (GEO) database (GSE100666), S100 calcium-binding protein A8 (S100A8) was highly expressed in RCC tissues. S100A8, an inflammatory regulatory factor, has emerged as an important mediator associated with the occurrence and development of cancer. Materials and Methods The Gene Expression Omnibus (GEO) database was used to identify the key genes and investigate the main signaling pathways in RCC. Human RCC samples and corresponding adjacent normal tissues were collected in our hospital. The expression of S100A8 in human RCC samples was detected using western blotting and immunohistochemical analysis. S100A8 overexpression or knockdown was mediated by using Lipofectamine 3000 in human renal cell carcinoma cell line 786-O and ACHN cells. Basic experiments, including MTT and cell apoptosis assays, were utilized for investigating the function of S100A8 in RCC. Furthermore, the levels of inflammation were also evaluated in 786-O and ACHN cells. Results In the current study, we found that downregulation of S100A8 inhibited proliferation and promoted apoptosis in 786-O and ACHN RCC cells. Of note, S100A8 silencing downregulated the phosphorylation of NF-κB p65, thereby decreasing the levels of TNF-α, cleaved caspase1, and MMP9. By contrast, S100A8 upregulation could increase these expressions. Conclusion Overall, S100A8 knockdown restrained RCC malignant biological properties, which was associated with the deactivation of the NF-κB signaling pathway. This present study demonstrates new insights that S100A8 may be a potential therapeutic target in RCC.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/0894-1939Test; https://doaj.org/toc/1521-0553Test

الوصول الحر: https://doaj.org/article/cca4f60f79974177a037f7ccd9bd4014Test