المؤلفون: Qian, Da, Liu, Yuxiang, Zheng, Jie, Cai, Jinquan

المصدر: Cell Death Discovery ; volume 10, issue 1 ; ISSN 2058-7716

مصطلحات موضوعية: Cancer Research, Cell Biology, Cellular and Molecular Neuroscience, Immunology

الوصف: Neurospagioma, arising from different glial cells such as astrocytes, oligodendrocytes, and ependymal cells, stands as the prevalent intracranial tumor within the central nervous system. Among its variants, glioblastoma (GBM) represents the most aggressive form, characterized by a notably high occurrence rate and a discouragingly low survival prognosis. The formidable challenge posed by glioblastoma underscores its critical importance as a life-threatening ailment. Currently, clinical approaches often involve surgical excision along with a combination of radiotherapy and chemotherapy. However, these treatments frequently result in a notable recurrence rate, accompanied by substantial adverse effects that significantly compromise the overall prognosis. Hence, there is a crucial need to investigate novel and dependable treatment strategies. Dendritic cells (DCs), being specialized antigen-presenting cells (APCs), hold a significant position in both innate and adaptive immune responses. Presently, DC vaccines have gained widespread application in the treatment of various tumors, including neurospoagioma. In this review, we summarize the immunomodulatory effects and related mechanisms of DC vaccines in neurospoagioma as well as the progress of clinical trials to propose possible challenges of DC vaccines and new development directions.

الإتاحة: https://doi.org/10.1038/s41420-023-01782-7Test
https://www.nature.com/articles/s41420-023-01782-7.pdfTest
https://www.nature.com/articles/s41420-023-01782-7Test

المؤلفون: Li, Shupeng, Li, Ziwei, Wang, Xinyu, Zhong, Junzhe, Yu, Daohan, Chen, Hao, Ma, Wenbin, Liu, Lingling, Ye, Minghuang, Shen, Ruofei, Jiang, Chuanlu, Meng, Xiangqi, Cai, Jinquan

المساهمون: Harbin Medical University Scientific Research Innovation Fund, National Natural Science Foundation of China, China Postdoctoral Science Foundation, Heilongjiang Postdoctoral Science Foundation, Natural Science Foundation of Heilongjiang Province, Heilongjiang Provincial Key R & D Project, Central Government Supporting Local University Reform and Development Fund for Excellent Youth Talents

المصدر: Cancer Cell International ; volume 23, issue 1 ; ISSN 1475-2867

مصطلحات موضوعية: Cancer Research, Genetics, Oncology

الوصف: Background Glioma is the most common and lethal type of brain tumor, and it is characterized by unfavorable prognosis and high recurrence rates. The reprogramming of energy metabolism and an immunosuppressive tumor microenvironment (TME) are two hallmarks of tumors. Complex and dynamic interactions between neoplastic cells and the surrounding microenvironment can generate an immunosuppressive TME, which can accelerate the malignant progression of glioma. Therefore, it is crucial to explore associations between energy metabolism and the immunosuppressive TME and to identify new biomarkers for glioma prognosis. Methods In our work, we analyzed the co-expression relationship between glycolytic genes and immune checkpoints based on the transcriptomic data from The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA) and found the correlation between HK3 expression and glioma tumor immune status. To investigate the biological role of HK3 in glioma, we performed bioinformatics analysis and established a mouse glioblastoma (GBM) xenograft model. Results Our study showed that HK3 significantly stimulated immune cell infiltration into the glioma TME. Tissue samples with higher HK3 expressive level showed increasing levels of immune cells infiltration, including M2 macrophages, neutrophils, and various subtypes of activated memory CD4 + T cells. Furthermore, HK3 expression was significantly increasing along with the elevated tumor grade, had a higher level in the mesenchymal subtype compared with those in other subtypes of GBM and could independently predict poor outcomes of GBM patients. Conclusion The present work mainly concentrated on the biological role of HK3 in glioma and offered a novel insight of HK3 regulating the activation of immune cells in the glioma microenvironment. These findings could provide a new theoretical evidence for understanding the metabolic molecular within the glioma microenvironment and identifying new therapeutic targets.

الإتاحة: https://doi.org/10.1186/s12935-023-03039-wTest

المؤلفون: Huang, Fanxuan, Wang, Xinyu, Zhong, Junzhe, Chen, Hao, Song, Dan, Xu, Tianye, Tian, Kaifu, Sun, Penggang, Sun, Nan, Qin, Jie, Song, Yu, Ma, Wenbin, Liu, Yuxiang, Yu, Daohan, Meng, Xiangqi, Jiang, Chuanlu, Xuan, Hanwen, Qian, Da, Cai, Jinquan

المساهمون: Heilongjiang Provincial Natural Science Foundation, China Postdoctoral Science Foundation, Heilongjiang Postdoctoral Science Foundation, Harbin Medical University Marshal Initiative Funding, National Natural Science Foundation of China, Heilongjiang Provincial Key R & D Project, Suzhou Youth Science and Technology Program, Heilongjiang Provincial Key Project of The Educational Science 14th Five-Year Plan, Heilongjiang Provincial Authority Fund for Returned Overseas Talents

المصدر: Cancer Cell International ; volume 23, issue 1 ; ISSN 1475-2867

مصطلحات موضوعية: Cancer Research, Genetics, Oncology

الوصف: Background N 6-methyladenosine (m6A), 5-methylcytosine (m5C) and N 1-methyladenosine (m1A) are the main RNA methylation modifications involved in the progression of cancer. However, it is still unclear whether RNA methylation-related long noncoding RNAs (lncRNAs) affect the prognosis of glioma. Methods We summarized 32 m6A/m5C/m1A-related genes and downloaded RNA-seq data and clinical information from The Cancer Genome Atlas (TCGA) database. Differential expression analysis and weighted gene co-expression network analysis (WGCNA) were used to identify differentially expressed (DE-) RNA methylation-related lncRNAs in order to construct a prognostic signature of glioma and in order to determine their correlation with immune function, immune therapy and drug sensitivity. In vitro and in vivo assays were performed to elucidate the effects of RNA methylation-related lncRNAs on glioma. Results A total of ten RNA methylation-related lncRNAs were used to construct a survival and prognosis signature, which had good independent prediction ability for patients. It was found that the high-risk group had worse overall survival (OS) than the low-risk group in all cohorts. In addition, the risk group informed the immune function, immunotherapy response and drug sensitivity of patients with glioma in different subgroups. Knockdown of RP11-98I9.4 and RP11-752G15.8 induced a more invasive phenotype, accelerated cell growth and apparent resistance to temozolomide (TMZ) both in vitro and in vivo. We observed significantly elevated global RNA m5C and m6A levels in glioma cells. Conclusion Our study determined the prognostic implication of RNA methylation-related lncRNAs in gliomas, established an RNA methylation-related lncRNA prognostic model, and elucidated that RP11-98I9.4 and RP11-752G15.8 could suppress glioma proliferation, migration and TMZ resistance. In the future, these RNA methylation-related lncRNAs may become a new choice for immunotherapy of glioma.

الإتاحة: https://doi.org/10.1186/s12935-023-03001-wTest

المؤلفون: Sun, Penggang, Wang, Xinyu, Zhong, Junzhe, Yu, Daohan, Xuan, Hanwen, Xu, Tianye, Song, Dan, Yang, Changxiao, Wang, Pandeng, Liu, Yuxiang, Meng, Xiangqi, Cai, Jinquan

المساهمون: National Natural Science Foundation of China, China Postdoctoral Science Foundation, Heilongjiang Postdoctoral Science Foundation

المصدر: Frontiers in Genetics ; volume 14 ; ISSN 1664-8021

الوصف: Background: Glioma is a highly heterogeneous disease, causing the prognostic prediction a challenge. Pyroptosis, a programmed cell death mediated by gasdermin (GSDM), is characterized by cell swelling and the release of inflammatory factors. Pyroptosis occurs in several types of tumor cells, including gliomas. However, the value of pyroptosis-related genes (PRGs) in the prognosis of glioma remains to be further clarified. Methods: In this study, mRNA expression profiles and clinical data of glioma patients were acquired from TCGA and CGGA databases, and one hundred and eighteen PRGs were obtained from the Molecular Signatures Database and GeneCards. Then, consensus clustering analysis was performed to cluster glioma patients. The least absolute shrinkage and selection operator (LASSO) Cox regression model was used to establish a polygenic signature. Functional verification of the pyroptosis-related gene GSDMD was achieved by gene knockdown and western blotting. Moreover, the immune infiltration status between two different risk groups were analyzed through the “gsva” R package. Results: Our results demonstrated that the majority of PRGs (82.2%) were differentially expressed between lower-grade gliomas (LGG) and glioblastoma (GBM) in the TCGA cohort. In univariate Cox regression analysis, eighty-three PRGs were shown to be associated with overall survival (OS). A five-gene signature was constructed to divide patients into two risk groups. Compared with patients in the low-risk group, patients in the high-risk group had obviously shorter OS ( p < 0.001). Also, we found that the high-risk group showed a higher infiltrating score of immune cells and immune-related functions. Risk score was an independent predictor of OS (HR > 1, p < 0.001). Furthermore, knockdown of GSDMD decreased the expression of IL-1β and cleaved caspase-1. Conclusion: Our study constructed a new PRGs signature, which can be used to predict the prognosis of glioma patients. Targeting pyroptosis might serve as a potential ...

الإتاحة: https://doi.org/10.3389/fgene.2023.1087563Test

المؤلفون: Xuan, Hanwen, Zhong, Junzhe, Wang, Xinyu, Song, Yu, Shen, Ruofei, Liu, Yuxiang, Zhang, Sijia, Cai, Jinquan, Liu, Meichen

المصدر: BMC Medical Education; 5/7/2024, Vol. 24 Issue 1, p1-9, 9p

مصطلحات موضوعية: DATABASES, ORGANS (Anatomy), TEACHING methods, BLOOD vessels, SURGICAL & topographical anatomy, UNDERGRADUATES

مستخلص: Background: In this era of rapid technological development, medical schools have had to use modern technology to enhance traditional teaching. Online teaching was preferred by many medical schools. However due to the complexity of intracranial anatomy, it was challenging for the students to study this part online, and the students were likely to be tired of neurosurgery, which is disadvantageous to the development of neurosurgery. Therefore, we developed this database to help students learn better neuroanatomy. Main body: The data were sourced from Rhoton's Cranial Anatomy and Surgical Approaches and Neurosurgery Tricks of the Trade in this database. Then we designed many hand gesture figures connected with the atlas of anatomy. Our database was divided into three parts: intracranial arteries, intracranial veins, and neurosurgery approaches. Each section below contains an atlas of anatomy, and gestures represent vessels and nerves. Pictures of hand gestures and atlas of anatomy are available to view on GRAVEN (www.graven.cn) without restrictions for all teachers and students. We recruited 50 undergraduate students and randomly divided them into two groups: using traditional teaching methods or GRAVEN database combined with above traditional teaching methods. Results revealed a significant improvement in academic performance in using GRAVEN database combined with traditional teaching methods compared to the traditional teaching methods. Conclusion: This database was vital to help students learn about intracranial anatomy and neurosurgical approaches. Gesture teaching can effectively simulate the relationship between human organs and tissues through the flexibility of hands and fingers, improving anatomy interest and education. [ABSTRACT FROM AUTHOR]

: Copyright of BMC Medical Education is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Chen, Hao, Xuan, Hanwen, Cai, Jinquan, Liu, Meichen, Shi, Lei

المصدر: BMC Medical Education; 4/25/2024, Vol. 24 Issue 1, p1-15, 15p

مصطلحات موضوعية: MEDICAL students, EMPATHY, CLINICAL competence, MEDICAL education, COMMUNICATIVE competence

مستخلص: Introduction: Empathy is considered the ability to understand or feel others emotions or experiences. As an important part of medical education, empathy can affect medical students in many ways. It is still lacking a comprehensive evaluation of the existing articles on empathy's impact on medical students, despite the existence of many articles on the topic. Objectives: To summarize the impact of empathy on medical students during medical education from four perspectives: mental health, academic performance, clinical competence, and specialty preference. Methods: The search terms used for retrieval were "empathy", "medical student", "mental health", "depression", "anxiety", "burnout", "examinations", "academic performance", "clinical competence", "specialty preference" on PubMed, EBSCO, and Web of Science before January 2024. The search was carried out by two reviewers. Titles and abstracts were screened independently and reviewed based on inclusion/exclusion criteria. A consensus was drawn on which articles were included. Results: Our results indicated that high empathy was a positive factor for mental health, However, students with high affective empathy were more likely to suffer from depression, anxiety, and burnout. Empathy was found to be unrelated to academic performance, but positively correlated with clinical competence, particularly in terms of communication skills. Medical students with high levels of empathy tended to prefer people-oriented majors. Conclusions: Medical students who score higher on the self-reported empathy scales often have better mental health, better communication skills, and tend to choose people-oriented specialties. But empathy is not related to academic performance. Additionally, the different dimensions of empathy have different impacts on medical students. It is necessary to design targeted courses and training for medical students to enhance their empathy. [ABSTRACT FROM AUTHOR]

: Copyright of BMC Medical Education is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Li, Shupeng, Dong, Jiawei, Wang, Xinyu, Meng, Xiangqi, Jiang, Chuanlu, Cai, Jinquan

المساهمون: National Natural Science Foundation of China, China Postdoctoral Science Foundation, Heilongjiang Provincial Key R & D Project, Central Government Supporting Local University Reform and Development Fund for Excellent Youth Talents, Heilongjiang Provincial Postdoctoral Science Foundation, Harbin Medical University Scientific Research Innovation Fund

المصدر: Chinese Neurosurgical Journal ; volume 8, issue 1 ; ISSN 2057-4967

مصطلحات موضوعية: Neurology (clinical), Neurology, Surgery

الوصف: TTFields is a novel treating modality of glioblastoma (GBM) which can significantly prolong the overall survival (OS) of newly diagnosed or recurrent glioblastoma. Some researchers have revealed that a variety of factors can affect the efficacy of TTFields. So, we review the available literature about the influencing factors on efficacy of TTFields and then choose two experimentally supported factors: the dose of dexamethasone and compliance of TTFields to perform a meta-analysis. The PubMed, Embase, and the Cochrane Library are searched. Five articles are identified between 2014 and 2017. Three articles are about the compliance of TTFields. Two articles are about the dose of dexamethasone. The Newcastle-Ottawa Quality Assessment Scale (NOS) is used as an assessment tool to evaluate the methodological quality of all included trials. The scale’s range varies from 0 to 9 stars. According to the Cochrane Handbook for Systematic Reviews of Interventions, articles are graded in six items to evaluate the risk of bias. Two reviewers rate the studies independently and the final decision is reached by consensus. Our data shows that the median OS is conspicuously longer in the TTFields group in which the dose of dexamethasone is ≤ 4.1 mg, WMD = 9.23 [95% CI 5.69–12.78]; P < 0.05). And the patients whose compliance of TTFields treatment ≥ 75% (≥ 18 h per day) have a significant lower overall survival risk than the patients whose compliance of TTFields treatment < 75% (HR = 0.57 [95% CI 0.46–0.70]; P < 0.00001).TTFields is a safe and efficient novel treatment modality. The dose of dexamethasone ≤ 4.1 mg of TTFields treatment and the compliance of TTFields treatment ≥ 75%, ≥ 18 h per day are beneficial to the prognosis of the glioblastoma patients.

الإتاحة: https://doi.org/10.1186/s41016-022-00294-0Test

المؤلفون: Zhao, Shihong, Xu, Boya, Ma, Wenbin, Chen, Hao, Jiang, Chuanlu, Cai, Jinquan, Meng, Xiangqi

المساهمون: National Natural Science Foundation of China, China Postdoctoral Science Foundation, Karolinska Institutet, Heilongjiang Provincial Postdoctoral Science Foundation, Second Affiliated Hospital of Harbin Medical University, Health and Family Planning Commission of Heilongjiang Province

المصدر: Frontiers in Immunology ; volume 12 ; ISSN 1664-3224

مصطلحات موضوعية: Immunology, Immunology and Allergy

الوصف: With the gradual understanding of tumor development, many tumor therapies have been invented and applied in clinical work, and immunotherapy has been widely concerned as an emerging hot topic in the last decade. It is worth noting that immunotherapy is nowadays applied under too harsh conditions, and many tumors are defined as “cold tumors” that are not sensitive to immunotherapy, and brain tumors are typical of them. However, there is much evidence that suggests a link between DNA damage repair mechanisms and immunotherapy. This may be a breakthrough for the application of immunotherapy in brain tumors. Therefore, in this review, first, we will describe the common pathways of DNA damage repair. Second, we will focus on immunotherapy and analyze the mechanisms of DNA damage repair involved in the immune process. Third, we will review biomarkers that have been or may be used to evaluate immunotherapy for brain tumors, such as TAMs, RPA, and other molecules that may provide a precursor assessment for the rational implementation of immunotherapy for brain tumors. Finally, we will discuss the rational combination of immunotherapy with other therapeutic approaches that have an impact on the DNA damage repair process in order to open new pathways for the application of immunotherapy in brain tumors, to maximize the effect of immunotherapy on DNA damage repair mechanisms, and to provide ideas and guidance for immunotherapy in brain tumors.

الإتاحة: https://doi.org/10.3389/fimmu.2021.829268Test

المؤلفون: Yang, Xiaofeng, Chen, Lin, Pu, Jiangbo, Li, Yusheng, Cai, Jinquan, Chen, Lukui, Feng, Shiqing, He, Jianghong, Wang, Yunliang, Zhang, Sai, Cheng, Shixiang, Huang, Hongyun

المصدر: Journal of Neurorestoratology ; volume 10, issue 2, page 100005 ; ISSN 2324-2426

الإتاحة: https://doi.org/10.1016/j.jnrt.2022.100005Test
https://api.elsevier.com/content/article/PII:S2324242622001012?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2324242622001012?httpAccept=text/plainTest

المؤلفون: Chen, Lukui, Zhang, Guilong, Feng, Shiqing, Xue, Mengzhou, Cai, Jinquan, Chen, Lin, Deng, Yubin, Wang, Yunliang

المصدر: Journal of Neurorestoratology ; volume 10, issue 2, page 100001 ; ISSN 2324-2426

الإتاحة: https://doi.org/10.1016/j.jnrt.2022.100001Test
https://api.elsevier.com/content/article/PII:S2324242622000973?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2324242622000973?httpAccept=text/plainTest