المؤلفون: Amokrane, Lucas, Pokotylo, Igor, Acket, Sébastien, Ducloy, Amélie, Troncoso-Ponce, Adrian, Cacas, Jean-Luc, Ruelland, Eric

المساهمون: Génie Enzymatique et Cellulaire (GEC), Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS), Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Conseil régional des Hauts-de-France, Programme PAUSE, AgroParisTech SURF-OMIQ APT-AO-2024-10

المصدر: ISSN: 2223-7747 ; Plants ; https://hal.science/hal-04599993Test ; Plants, 2024, 13, pp.1532. ⟨10.3390/plants13111532⟩.

مصطلحات موضوعية: crop species, lipid signaling, phosphatidic acid, phospholipase, diacylglycerol kinase, environmental stresses, signaling, protein structure, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM]

الوصف: International audience ; In plant models such as Arabidopsis thaliana, phosphatidic acid (PA), a key molecule of lipid signaling, was shown not only to be involved in stress responses, but also in plant development and nutrition. In this article, we highlight lipid signaling existing in crop species. Based on open access databases, we update the list of sequences encoding phospholipases D, phosphoinositide-dependent phospholipases C, and diacylglycerol-kinases, enzymes that lead to the production of PA. We show that structural features of these enzymes from model plants are conserved in equivalent proteins from selected crop species. We then present an in-depth discussion of the structural characteristics of these proteins before focusing on PA binding proteins. For the purpose of this article, we consider RESPIRATORY BURST OXIDASE HOMOLOGUEs (RBOHs), the most documented PA target proteins. Finally, we discuss pioneering experiments that show, by different approaches such as monitoring of gene expression, use of pharmacological agents, ectopic over-expression of genes, and the creation of silenced mutants, that lipid signaling plays major roles in crop species. Finally, we present major open questions that require attention since we have only a perception of the peak of the iceberg when it comes to the exciting field of phospholipid signaling in plants.

العلاقة: hal-04599993; https://hal.science/hal-04599993Test; https://hal.science/hal-04599993/documentTest; https://hal.science/hal-04599993/file/amokrane%20et%20al.,%202024,%20plants.pdfTest

الإتاحة: https://doi.org/10.3390/plants13111532Test
https://hal.science/hal-04599993Test
https://hal.science/hal-04599993/documentTest
https://hal.science/hal-04599993/file/amokrane%20et%20al.,%202024,%20plants.pdfTest

المؤلفون: Ducloy, Amélie, Azzopardi, Marianne, Ivsic, Caroline, Cueff, Gwendal, Sourdeval, Delphine, Charif, Delphine, Cacas, Jean-Luc

المساهمون: Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), The University of Western Australia (UWA), Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA)

المصدر: ISSN: 2352-3409 ; Data in Brief ; https://hal.inrae.fr/hal-04566255Test ; Data in Brief, 2024, 53, pp.110243. ⟨10.1016/j.dib.2024.110243⟩.

مصطلحات موضوعية: Protein homeostasis, Asparagine-linked glycosylation, Inositol-requiring enzyme-1, Tunicamycin Biological sciences, Plants, Shoots, Roots, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology

الوصف: International audience ; The Unfolded Protein Response (UPR) is a retrograde, ER-to-nucleus, signalling pathway which is conserved across kingdoms. In plants, it contributes to development, reproduction, immunity and tolerance to abiotic stress. This RNA sequencing (RNA-seq) dataset was produced from 14-day-old Arabidopsis thaliana seedlings challenged by tunicamycin (Tm), an antibiotic inhibiting Asn-linked glycosylation in the endoplasmic reticulum (ER), causing an ER stress and eventually activating the UPR. Wild-type (WT) and a double mutant deficient for two main actors of the UPR (INOSITOL-REQUIRING ENZYME 1A and INOSITOL-REQUIRING ENZYME 1B) were used as genetic backgrounds in our experimental setup, allowing to distinguish among differentially-expressed genes (DEGs) which ones are dependent on or independent on IRE1s. Also, shoots and roots were harvested separately to determine organ-specific transcriptomic responses to Tm. Library and sequencing were performed using DNBseq™ technology by the Beijing Genomics Institute. Reads were mapped and quantified against the Arabidopsis genome. Differentially-expressed genes were identified using Rflomics upon filtering and normalization by the Trimmed Mean of M-value (TMM) method. While the genotype effect was weak under mock conditions (with a total of 182 DEGs in shoots and 195 DEGs in roots), the tunicamycin effect on each genotype was characterized by several hundred of DEGs in both shoots and roots. Among these genes, 872 and 563 genes were statistically up- and down-regulated in the shoot tissues of the double mutant when compared to those of WT, respectively. In roots of Tm-challenged seedlings, 425 and 439 genes were significantly up- and down-regulated in mutants with respect to WT. We believe that our dataset could be reused for investigating any biological questions linked to ER homeostasis and its role in plant physiology.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/38533111; hal-04566255; https://hal.inrae.fr/hal-04566255Test; https://hal.inrae.fr/hal-04566255/documentTest; https://hal.inrae.fr/hal-04566255/file/2024_Ducloy_Data-in-Brief.pdfTest; PUBMED: 38533111; WOS: 001204917600001

الإتاحة: https://doi.org/10.1016/j.dib.2024.110243Test
https://hal.inrae.fr/hal-04566255Test
https://hal.inrae.fr/hal-04566255/documentTest
https://hal.inrae.fr/hal-04566255/file/2024_Ducloy_Data-in-Brief.pdfTest

المؤلفون: Kacprzyk, Joanna, Burke, Rory, Armengot, Laia, Coppola, Marianna, Tattrie, Sophie B., Vahldick, Hannah, Bassham, Diane C., Bosch, Maurice, Brereton, Nicholas J. B., Cacas, Jean‐Luc, Coll, Núria S., Gallois, Patrick, Kuchitsu, Kazuyuki, Nowack, Moritz, Rogers, Hilary J., Van Breusegem, Frank, Gunawardena, Arunika H. L. A. N., McCabe, Paul F.

المصدر: New Phytologist ; ISSN: 0028-646X ; ISSN: 1469-8137

مصطلحات موضوعية: Biology and Life Sciences, Plant Science, Physiology, New Phytologist Workshop, abiotic stress, biotic stress, model systems, plant cell death, plant development, plant programmed cell death

الوصف: Programmed cell death (PCD) is fundamentally important for plant development, abiotic stress responses and immunity, but our understanding of its regulation remains fragmented. Building a stronger research community is required to accelerate progress in this area through knowledge exchange and constructive debate. In this Viewpoint, we aim to initiate a collective effort to integrate data across a diverse set of experimental models to facilitate characterisation of the fundamental mechanisms underlying plant PCD and ultimately aid the development of a new plant cell death classification system in the future. We also put forward our vision for the next decade of plant PCD research stemming from discussions held during the 31st New Phytologist workshop, 'The Life and Death Decisions of Plant Cells' that took place at University College Dublin in Ireland (14-15 June 2023). We convey the key areas of significant progress and possible future research directions identified, including resolving the spatiotemporal control of cell death, isolation of its molecular and genetic regulators, and harnessing technical advances for studying PCD events in plants. Further, we review the breadth of potential impacts of plant PCD research and highlight the promising new applications of findings from this dynamically evolving field.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/01HTPWM576APP7F1KXTPTNVEJDTest; http://hdl.handle.net/1854/LU-01HTPWM576APP7F1KXTPTNVEJDTest; http://doi.org/10.1111/nph.19709Test; https://biblio.ugent.be/publication/01HTPWM576APP7F1KXTPTNVEJD/file/01HTPWNW3SMQ4X1VTJ3ZSF2KDJTest

الإتاحة: https://doi.org/10.1111/nph.19709Test
https://biblio.ugent.be/publication/01HTPWM576APP7F1KXTPTNVEJDTest
http://hdl.handle.net/1854/LU-01HTPWM576APP7F1KXTPTNVEJDTest
https://biblio.ugent.be/publication/01HTPWM576APP7F1KXTPTNVEJD/file/01HTPWNW3SMQ4X1VTJ3ZSF2KDJTest

المؤلفون: Havé, Marien, Luo, Jie, Tellier, Frédérique, Balliau, Thierry, Cueff, Gwendal, Chardon, Fabien, Zivy, Michel, Rajjou, Loic, Cacas, Jean-Luc, Masclaux-Daubresse, Céline

المصدر: The New Phytologist, 2019 Aug 01. 223(3), 1461-1477.

الوصول الحر: https://www.jstor.org/stable/26759488Test

المؤلفون: Deruyffelaere, Carine, Purkrtova, Zita, Bouchez, Isabelle, Collet, Boris, Cacas, Jean-Luc, Chardot, Thierry, Gallois, Jean-Luc, D’Andrea, Sabine

المصدر: Plant Cell, 2018 Sep 01. 30(9), 2116-2136.

الوصول الحر: https://www.jstor.org/stable/90025554Test

المؤلفون: Gomez, Rodrigo, Enrique, Lupette, Josselin, Chambaud, Clément, Castets, Julie, Ducloy, Amélie, Cacas, Jean-Luc, Masclaux-Daubresse, Céline, Bernard, Amélie

المساهمون: Laboratoire de biogenèse membranaire (LBM), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), This work was supported by the European Research Council (ERC) under European Union’s Horizon 2020 research and innovation program (grant agreement No 852136, LIP-ATG to AB). The IJPB benefits from the support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007)., ANR-17-EURE-0007,SPS-GSR,Ecole Universitaire de Recherche de Sciences des Plantes de Paris-Saclay(2017), European Project: 852136,LIP-ATG

المصدر: ISSN: 2073-4409 ; Cells ; https://agroparistech.hal.science/hal-03883291Test ; Cells, 2021, 10 (6), pp.1272. ⟨10.3390/cells10061272⟩.

مصطلحات موضوعية: autophagy, environmental stresses, lipids, ATG proteins, autophagosomes, ER-stress, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Throughout their life cycle, plants face a tremendous number of environmental and developmental stresses. To respond to these different constraints, they have developed a set of refined intracellular systems including autophagy. This pathway, highly conserved among eukaryotes, is induced by a wide range of biotic and abiotic stresses upon which it mediates the degradation and recycling of cytoplasmic material. Central to autophagy is the formation of highly specialized double membrane vesicles called autophagosomes which select, engulf, and traffic cargo to the lytic vacuole for degradation. The biogenesis of these structures requires a series of membrane remodeling events during which both the quantity and quality of lipids are critical to sustain autophagy activity. This review highlights our knowledge, and raises current questions, regarding the mechanism of autophagy, and its induction and regulation upon environmental stresses with a particular focus on the fundamental contribution of lipids. How autophagy regulates metabolism and the recycling of resources, including lipids, to promote plant acclimation and resistance to stresses is further discussed.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/34063958; info:eu-repo/grantAgreement//852136/EU/The missing link: how do membrane lipids interplay with ATG proteins to instruct plant autophagy/LIP-ATG; hal-03883291; https://agroparistech.hal.science/hal-03883291Test; https://agroparistech.hal.science/hal-03883291/documentTest; https://agroparistech.hal.science/hal-03883291/file/cells-10-01272vf.pdfTest; PUBMED: 34063958; WOS: 000665365300001

الإتاحة: https://doi.org/10.3390/cells10061272Test
https://agroparistech.hal.science/hal-03883291Test
https://agroparistech.hal.science/hal-03883291/documentTest
https://agroparistech.hal.science/hal-03883291/file/cells-10-01272vf.pdfTest

المؤلفون: D’andrea, Sabine, Deruyffelaere, Carine, C., Bouchez, Isabelle, Cacas, Jean-Luc, Gallois, Jean-Luc

المساهمون: Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Génétique et Amélioration des Fruits et Légumes (GAFL), Institut National de la Recherche Agronomique (INRA)

المصدر: 9th European Symposium on Plant Lipids
https://hal.science/hal-04518499Test
9th European Symposium on Plant Lipids, Jul 2019, Marseille, France

مصطلحات موضوعية: [SDV]Life Sciences [q-bio]

جغرافية الموضوع: Marseille, France

الوصف: International audience ; Seed lipid droplets (LDs) of many dicotyledonous plants, including Arabidopsis thaliana, are a good model to investigate the mechanisms controlling LD dynamics. Indeed, LD degradation occurs rapidly and massively in germinated seeds, to provide carbon and energy for seedling growth. Post-germinative mobilization of storage lipids is preceded by the degradation of oleosins, the major structural LD proteins which stabilize LDs in dry seeds. We previously showed that oleosins are marked for degradation by ubiquitination, and extracted from LDs before proteolysis. The mechanisms underlying the dislocation of these LD-anchored proteins from the phospholipid monolayer are yet unknown. We found that a protein called PUX10 is required for proper extraction of ubiquitinated oleosins from the LD surface in young Arabidopsis seedlings. PUX10 localizes to LDs and binds to ubiquitinated proteins. PUX10 also interacts with CDC48A, the ATPase which selectively extract misfolded proteins from the ER in the degradation process known as ERAD (for ER-associated degradation). We propose that PUX10 is an adaptor protein recruiting CDC48A to ubiquitinated oleosins, thus promoting the extraction of oleosins from LDs by the segregase activity of CDC48A. PUX10 and CDC48A are core components of a new LD-associated machinery that we named LD-associated degradation (LDAD) system. Moreover, we observed that PUX10 and CDC48A localize to a specific subpopulation of LDs in germinated seeds, suggesting a functional differentiation of LDs in plants. We also showed that a deubiquitinating enzyme known for its role in intracellular trafficking and vacuolar biogenesis is necessary for the ubiquitination of oleosins.

العلاقة: hal-04518499; https://hal.science/hal-04518499Test; https://hal.science/hal-04518499v2/documentTest; https://hal.science/hal-04518499v2/file/20190707_EuroPlantLipids_Marseille_DAndrea.pdfTest

الإتاحة: https://hal.science/hal-04518499Test
https://hal.science/hal-04518499v2/documentTest
https://hal.science/hal-04518499v2/file/20190707_EuroPlantLipids_Marseille_DAndrea.pdfTest

المؤلفون: D’andrea, Sabine, Deruyffelaere, Carine, C., Bouchez, Isabelle, Cacas, Jean-Luc, Gallois, Jean-Luc

المساهمون: Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Génétique et Amélioration des Fruits et Légumes (GAFL), Institut National de la Recherche Agronomique (INRA)

المصدر: 9th European Symposium on Plant Lipids
https://hal.science/hal-04518499Test
9th European Symposium on Plant Lipids, Jul 2019, Marseille, France

مصطلحات موضوعية: [SDV]Life Sciences [q-bio]

جغرافية الموضوع: Marseille, France

الوصف: International audience

العلاقة: hal-04518499; https://hal.science/hal-04518499Test; https://hal.science/hal-04518499/documentTest; https://hal.science/hal-04518499/file/20190707_EuroPlantLipids_Marseille_DAndrea.pdfTest

الإتاحة: https://hal.science/hal-04518499Test
https://hal.science/hal-04518499/documentTest
https://hal.science/hal-04518499/file/20190707_EuroPlantLipids_Marseille_DAndrea.pdfTest

المؤلفون: Masclaux‐daubresse, Céline, D’andrea, Sabine, Bouchez, Isabelle, Cacas, Jean-Luc

المساهمون: Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ANR-17-EURE-0007,SPS-GSR,Ecole Universitaire de Recherche de Sciences des Plantes de Paris-Saclay(2017)

المصدر: ISSN: 0022-0957.

مصطلحات موضوعية: Autophagy, beta-oxidation, endoplasmic reticulum, leaf senescence, lipidomic, lipid droplet, lipids, oil body, oleosome, triacylglycerol, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM]

الوصف: International audience ; Autophagy is a universal mechanism that facilitates the degradation of unwanted cytoplasmic components in eukaryotic cells. In this review, we highlight recent developments in the investigation of the role of autophagy in lipid homeostasis in plants by comparison with algae, yeast, and animals. We consider the storage compartments that form the sources of lipids in plants, and the roles that autophagy plays in the synthesis of triacylglycerols and in the formation and maintenance of lipid droplets. We also consider the relationship between lipids and the biogenesis of autophagosomes, and the role of autophagy in the degradation of lipids in plants.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/32080724; hal-03659044; https://agroparistech.hal.science/hal-03659044Test; https://agroparistech.hal.science/hal-03659044/documentTest; https://agroparistech.hal.science/hal-03659044/file/2020%20J%20Exp%20Bot%20Masclaux.pdfTest; PUBMED: 32080724; WOS: 000538790700004

الإتاحة: https://doi.org/10.1093/jxb/eraa082Test
https://agroparistech.hal.science/hal-03659044Test
https://agroparistech.hal.science/hal-03659044/documentTest
https://agroparistech.hal.science/hal-03659044/file/2020%20J%20Exp%20Bot%20Masclaux.pdfTest

المؤلفون: Cacas, Jean-Luc, Buré, Corinne, Grosjean, Kevin, Gerbeau-Pissot, Patricia, Lherminier, Jeannine, Rombouts, Yoann, Maes, Emmanuel, Bossard, Claire, Gronnier, Julien, Furt, Fabienne, Fouillen, Laetitia, Germain, Véronique, Bayer, Emmanuelle, Cluzet, Stéphanie, Robert, Franck, Schmitter, Jean-Marie, Deleu, Magali, Lins, Laurence, Simon-Plas, Françoise, Mongrand, Sébastien

المصدر: Plant Physiology, 2016 Jan 01. 170(1), 367-384.

الوصول الحر: https://www.jstor.org/stable/24807230Test