المؤلفون: J. Giraud, M. Foroutan, J. Boubaker-Vitre, F. Grillet, Z. Homayed, U. Jadhav, P. Crespy, C. Breuker, J-F. Bourgaux, J. Hazerbroucq, C. Pignodel, B. Brulin, R.A. Shivdasani, P. Jay, F. Hollande, J. Pannequin

المصدر: Translational Oncology, Vol 14, Iss 2, Pp 101001- (2021)

مصطلحات موضوعية: Lgr5 (GPR49), Progastrin, Intestinal stem cells, Adenomas, Tumor initiating cells, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Progastrin is an unprocessed soluble peptide precursor with a well-described tumor-promoting role in colorectal cancer. It is expressed at small levels in the healthy intestinal mucosa, and its expression is enhanced at early stages of intestinal tumor development, with high levels of this peptide in hyperplastic intestinal polyps being associated with poor neoplasm-free survival in patients. Yet, the precise type of progastrin-producing cells in the healthy intestinal mucosa and in early adenomas remains unclear. Here, we used a combination of immunostaining, RNAscope labelling and retrospective analysis of single cell RNAseq results to demonstrate that progastrin is produced within intestinal crypts by a subset of Bmi1+/Prox1+/LGR5low endocrine cells, previously shown to act as replacement stem cells in case of mucosal injury. In contrast, our findings indicate that intestinal stem cells, specified by expression of the Wnt signaling target LGR5, become the main source of progastrin production in early mouse and human intestinal adenomas. Collectively our results suggest that the previously identified feed-forward mechanisms between progastrin and Wnt signaling is a hallmark of early neoplastic transformation in mouse and human colonic adenomas.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S1936523320304939Test; https://doaj.org/toc/1936-5233Test

الوصول الحر: https://doaj.org/article/49e193f5170640d2bfda38cb18e81de5Test

المؤلفون: Nicolas Pirro, G. Godlewski, Bernard Sastre, Mehdi Ouaissi, Pierre Champsaur, T. Le Corroller, C. Pignodel, Aude Lagier, Igor Sielezneff, Michel Prudhomme

المصدر: Morphologie. 94:9-12

مصطلحات موضوعية: Gynecology, medicine.medical_specialty, business.industry, medicine, Neoplasm staging, Anatomy, business

الوصف: Resume L’envahissement des nœuds lymphatiques (NL) du rectum est un facteur pronostique essentiel pour les malades ayant un cancer du rectum. Malgre une meilleure connaissance des mecanismes de propagation des cancers colorectaux, le drainage lymphatique du rectum reste mal connu. Le but de ce travail etait de determiner le nombre de NL situes entre l’origine et la terminaison de l’artere rectale superieure et d’evaluer la frequence du NL de Mondor. Patients et methodes Vingt-cinq sujets anatomiques ont ete etudies. Les prelevements etaient faits par la technique de l’exerese totale du mesorectum. Les NL etaient recherches par methode manuelle dans le tissu cellulolymphatique entourant l’artere rectale superieure jusqu’a 2 cm sous sa terminaison. Tous les prelevements avaient une analyse histologique. La correlation entre le nombre de NL et le volume et le poids du tissu entourant l’artere rectale superieure etait evalue par un test non parametrique. Resultats Le nombre moyen de NL situes entre l’origine et la terminaison de l’artere rectale superieure etait de 2,8 ± 1,4. La taille des NL variait entre 1 et 7 mm. La plupart des NL mesuraient moins de 3 mm (56 %). Sept sujets avaient un ganglion de Mondor. La taille moyenne du ganglion de Mondor etait de 3,4 ± 2,1 cm. Conclusion Le nombre de NL situes autour de l’artere rectale superieure est faible, variant entre 1 et 5. Le ganglion de Mondor est un NL inconstant du rectum dont la seule caracteristique est sa localisation dans la bifurcation ou trifurcation de l’artere rectale superieure.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::5cb53e67975f4aa7923ddb824f348c90Test
https://doi.org/10.1016/j.morpho.2009.11.001Test

المؤلفون: M Prudhomme, H Ould Said, C Pignodel, H Emptas, J Poilleux, G. Godlewski, O Philippe

المصدر: Annales de Chirurgie. 125:844-849

مصطلحات موضوعية: Gynecology, medicine.medical_specialty, Tomography x ray computed, business.industry, Treatment outcome, medicine, Surgery, Rectal diseases, business

الوصف: Resume But de l’etude : Cette etude retrospective avait pour but de rapporter trois observations de kyste vestigial retrorectal (KVR). Patients et methode : De 1977 a 1999, trois cas de KVR ont ete observes chez des femmes de 28, 57 et 53 ans, reveles par des douleurs ( n = 2) survenues dans un cas au cours d’une grossesse et par une occlusion intestinale aigue ( n = 1). Ces patientes ont ete operees par voie perineale dans deux cas et par voie abdominale dans un cas. Resultats : Il s’agissait d’un kyste epidermoide ( n = 1) et d’un kyste mixte ( n = 2) sans signe de malignite. Les suites operatoires ont ete simples dans deux cas et compliquees dans le 3 e par une fistule ureterovaginale qui a necessite une reimplantation ureterovesicale. Les deux premieres patientes revues avec un an de recul n’avaient aucun trouble et aucune recidive decelable. Conclusion : Les kystes vestigiaux retrorectaux sont des tumeurs rares qui risquent de degenerer. L’imagerie par scanner ou resonance magnetique et par echographie endorectale precise leur structure et leur topographie et guide leur abord chirurgical par voie perineale ou abdominale. En l’absence de degenerescence, l’exerese monobloc, si possible sans ouverture de la paroi kystique, donne de bons resultats.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::5ca331d9c320d263e2778690a375ce11Test
https://doi.org/10.1016/s0003-3944Test(00)00016-x

المؤلفون: S Bastide, C Pignodel, P Pouderoux, A Thibault

المصدر: Endoscopy. 45

مصطلحات موضوعية: Gynecology, medicine.medical_specialty, business.industry, Gastroenterology, Medicine, business

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::8bde8e3a2c87037c3ca07ffa88a048dfTest
https://doi.org/10.1055/s-0033-1336114Test

المؤلفون: Francis Poulat, C. Rammah, J. Giraud, Rana Abdel-Samad, Corinne Quittau-Prévostel, C Bonnans, Philippe Blache, H. Zalzali, C. Naudin, Sébastien Dupasquier, S Lumbroso, Pierre Raynaud, C Pignodel, Philippe Fort, Brigitte Boizet-Bonhoure, K Mouzat

المساهمون: Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biochimie [CHRU Nîmes], Centre Hospitalier Universitaire de Nîmes (CHU Nîmes), Centre de recherches de biochimie macromoléculaire (CRBM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-IFR122-Centre National de la Recherche Scientifique (CNRS)

المصدر: Oncogene
Oncogene, Nature Publishing Group, 2011, 30 (22), pp.2493-2503. ⟨10.1038/onc.2010.621⟩

مصطلحات موضوعية: Cancer Research, medicine.medical_specialty, Cell signaling, Protein Kinase C-alpha, Colorectal cancer, Molecular Sequence Data, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Biology, Adenocarcinoma, medicine.disease_cause, [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, Malignant transformation, 03 medical and health sciences, Transactivation, Mice, 0302 clinical medicine, Internal medicine, Cell Line, Tumor, Genetics, medicine, Animals, Humans, Promoter Regions, Genetic, Molecular Biology, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Genes, Dominant, 0303 health sciences, [SDV.GEN]Life Sciences [q-bio]/Genetics, Base Sequence, Wnt signaling pathway, Cancer, SOX9 Transcription Factor, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, medicine.disease, Introns, Mice, Mutant Strains, 3. Good health, Wnt Proteins, Alternative Splicing, Endocrinology, [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis, 030220 oncology & carcinogenesis, Colonic Neoplasms, Mutation, Cancer research, Carcinogenesis

الوصف: Inherited and acquired changes in pre-mRNA processing have significant roles in human diseases, especially cancer. Characterization of aberrantly spliced mRNAs may thus contribute to understand malignant transformation. We recently reported an anti-oncogenic potential for the SOX9 transcription factor in the colon. For instance, the Sox9 gene knock out in the mouse intestine results in an excess of proliferation with appearance of hyperplasia. SOX9 is expressed in colon cancer cells but its endogenous activity is weak. We looked for SOX9 variants that may impair SOX9 activity in colon cancer cells and we discovered MiniSOX9, a truncated version of SOX9 devoid of transactivation domain as a result of retention of the second intron. A significant overexpression of MiniSOX9 mRNA in human tumor samples compared with their matched normal tissues was observed by real-time reverse transcriptase-PCR. Immunohistochemistry revealed that MiniSOX9 is expressed at high levels in human colon cancer samples whereas it is undetectable in the surrounding healthy tissues. Finally, we discovered that MiniSOX9 behaves as a SOX9 inhibitor, inhibits protein kinase Cα promoter activity and stimulates the canonical Wnt pathway. This potential oncogenic activity of the SOX9 locus gives new insights on its role in colon cancer.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ac2b34c3807884398e71590adaf4ee8Test
https://hal.archives-ouvertes.fr/hal-00615517Test

المؤلفون: N, Pirro, T, Le Corroller, A, Lagier, M, Ouaissi, I, Sielezneff, C, Pignodel, G, Godlewski, B, Sastre, P, Champsaur, M, Prudhomme

المصدر: Morphologie : bulletin de l'Association des anatomistes. 94(305)

مصطلحات موضوعية: Male, Mesenteric Artery, Superior, Rectal Neoplasms, Dissection, Humans, Female, Mesenteric Artery, Inferior, Lymph Nodes, Neoplasm Staging

الوصف: Lymph node involvement is one of the most significant prognostic factors of patients with rectal cancer. Despite major advances in our understanding of the propagation of the rectal cancer, the lymphatic drainage of the rectum remains unclear. This study was designed to assess the number of lymph nodes located around the superior rectal artery and to assess the frequency of Mondor's lymph nodes.Twenty-five anatomic subjects were studied. All resections were performed using total mesorectal excision. Lymph nodes were sought in the tissue surrounding the superior rectal artery up to 2 cm under the ending of the superior rectal artery by manual dissection and were submitted for histological examination. The correlation between the number of lymph nodes, and the volume and weight of the tissue surrounding the superior rectal artery was evaluated by non-parametric Spearman test.The mean number of lymph nodes per specimen was 2.7 +/- 1.4. The size of the lymph nodes varied between 1 and 7 mm. The lymph nodes were mostly smaller than 3 mm (56%). The number of lymph nodes in the superior rectal mesentery was independent of its volume and its weight. Seven subjects had a Mondor's lymph node. The mean size of Mondor's lymph node was 3.4 +/- 2.1 cm.The number of NL located around the superior rectal artery is small, varying between 1 and 5. The Mondor's lymph node is an inconstant rectal NL. Its only characteristic is its location in the bifurcation or trifurcation of the superior rectal artery.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::dcdec70946745bdb27872b80f2e497bbTest
https://pubmed.ncbi.nlm.nih.gov/20149707Test

المؤلفون: J.P. Balducchi, J. Fabre, C. Barbuat, C. Pignodel, J. Fourcade, J. Jourdan, J.M. Joujoux, A. Dubois, F. Flaisler

المصدر: La Revue de Médecine Interne. 12:295-298

مصطلحات موضوعية: Gynecology, medicine.medical_specialty, business.industry, Gastroenterology, Internal Medicine, Medicine, business

الوصف: Resume L'hypertension portale secondaire a une hypervitaminose A n'a que rarement ete bien documentee. Les auteurs rapportent deux observations. Dans la premiere observation, une femme de 39 ans a absorbe pour bronzer de la meladinine (8 methoxypsoralene). Dans la seconde observation, une femme de 43 ans a absorbe pour maigrir du Plethoryl®. Les 2 observations associent des lesions histologiques hepatiques evocatrices de surcharge en vitamine A (hypertrophie des cellules de Ito, fibrose perisinusoidale, autofluorescence spontanee), un gradient de pression veineuse sus-hepatique egal ou superieur a 10 mmHg, des concentrations hepatiques de Vitamine A elevees. Si la responsabilite du Plethoryl® ne fait aucun doute dans l'observation 2, le mecanisme de l'hypervitaminose dans l'observation 1 est discute. Une revue de la litterature permet de preciser les signes des surcharges hepatiques en vitamine A, dont la frequence est probablement sous-estimee. Devant une hypertension portale dont l'etiologie reste inconnue, une hypervitaminose A doit etre recherchee.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::e475c56363cf5d2d65b534d99afdd6c3Test
https://doi.org/10.1016/s0248-8663Test(05)82867-x

المؤلفون: Pascale Fabbro-Peray, N. Pirro, C. Pignodel, P. Cathala, Michel Prudhomme, G. Godlewski

المصدر: Surgical and radiologic anatomy : SRA. 30(4)

مصطلحات موضوعية: Male, medicine.medical_specialty, Pathology, Colorectal cancer, Rectum, Mesorectum, Statistics, Nonparametric, Pathology and Forensic Medicine, medicine, Humans, Radiology, Nuclear Medicine and imaging, Mesentery, Lymph node, Mesorectal, Aged, Aged, 80 and over, business.industry, Rectal Neoplasms, Dissection, Cancer, Organ Size, Weights and Measures, medicine.disease, medicine.anatomical_structure, Lymphatic Metastasis, Surgery, Female, Lymph, Radiology, Lymph Nodes, Anatomy, business

الوصف: Lymph node involvement is one of the most significant prognostic factors of patients with rectal cancer. However, the distribution of mesorectal lymph nodes is not well known. This study was designed to assess lymph nodes in the mesorectum and to evaluate the correlation between the volume and weight of the mesorectum and the number of lymph nodes.The mesorectums of 20 human cadavers were studied. The volume and weight of the superior rectal mesentery, superior mesorectum and inferior mesorectum were measured. Lymph nodes were sought by manual dissection and were submitted for histological examination. The correlation between the number of lymph nodes and the volume and weight of the mesorectum was evaluated by non-parametric Spearman test.A total of 178 lymph nodes were identified. The mean number of lymph nodes per specimen was 9.2 +/- 4.5. The lymph nodes were mostly smaller than 3 mm and located in the superior and posterior parts of the mesorectum. A positive correlation was found between the number of mesorectal lymph nodes and the volume and weight of the mesorectum. The number of lymph nodes in the superior rectal mesentery was independent of its volume and its weight.Mesorectal lymph nodes are mainly located above the peritoneal reflection within the posterior mesorectum. The positive correlation between the volume or the weight of the mesorectum and the number of mesorectal lymph nodes should be considered as a possible means to determine the minimum number of mesorectal lymph nodes required for histological examination.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ecad4145ce18e6b9aa34c72245461288Test
https://pubmed.ncbi.nlm.nih.gov/18309450Test

المؤلفون: G, Godlewski, O, Philippe, H, Ould Said, M, Prudhomme, J, Poilleux, C, Pignodel, H, Emptas

المصدر: Annales de chirurgie. 125(9)

مصطلحات موضوعية: Adult, Biopsy, Epidermal Cyst, Pain, Middle Aged, Magnetic Resonance Imaging, Rectal Diseases, Treatment Outcome, Risk Factors, Acute Disease, Humans, Female, Tomography, X-Ray Computed, Intestinal Obstruction, Retrospective Studies

الوصف: The aim of this retrospective study was to report three cases of retrorectal vestigial cyst in adults.From 1977 to 1999 retrorectal vestigial cyst (RVC) was diagnosed in our department in three women who were 28, 57 and 53 years of age, respectively. RVC was revealed by either pain (n = 2) that occurred in one case in a pregnant woman, or acute intestinal obstruction (n = 1). The patients were operated on using a perineal approach in two cases and an abdominal approach in one case.One epidermoid cyst and two mixed cysts without any sign of malignancy were observed. Postoperative follow-up was simple in two patients, while complications occurred in the third one via an uretero-vaginal fistula which required uretero-vesical reimplantation. The first two patients, reviewed after a 1-year follow-up, had no functional trouble and no sign of recurrence.Retrorectal vestigial cysts are very rare tumors with a risk for degeneration. Computerized tomography on nuclear magnetic resonance and endorectal ultrasonography allow detection of their structure and topography and help guide their surgical approach. In the absence of malignancy, wide excision, if possible without opening of the cystic wall, leads to good results.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::a7f34fd315dc3675c8c8367d5f8f1f27Test
https://pubmed.ncbi.nlm.nih.gov/11244591Test

المؤلفون: A, Polge, J F, Bourgaux, E, Bancel, C, Pignodel, J C, Boyer, S, Poirey, B M, de Bornier, J L, Balmes, J P, Bali

المصدر: Digestive diseases and sciences. 43(7)

مصطلحات موضوعية: Aged, 80 and over, Male, Enzyme-Linked Immunosorbent Assay, DNA, Neoplasm, Adenocarcinoma, Middle Aged, Genes, p53, Polymerase Chain Reaction, Case-Control Studies, Mutation, Biomarkers, Tumor, Humans, Female, Tumor Suppressor Protein p53, Colorectal Neoplasms, Polymorphism, Single-Stranded Conformational, Aged, Follow-Up Studies

الوصف: Circulating p53 antibodies (ELISA method), p53 genetic alterations (SSCP), and protein overexpression (immunohistochemistry) were studied in 41 patients with colorectal adenocarcinomas and 10 control patients. Carcinoembryonic antigen (CEA) and carbohydrate antigen 19.9 (CA 19-9) were evaluated in parallel. Ten patients with p53 antibodies and p53 overexpression were selected. Tumor DNA extracts from these 10 patients were analyzed by SSCP. Of all 41 patients, 10 (24%) showed significant levels of p53 antibodies, and p53 accumulation was detected in 20 (48%) patients. In six patients, p53 antibody concentrations decreased rapidly after surgery; in two patients, these levels returned to normal values. Of the 10 selected tumors, eight revealed TP53 gene mutations. Only two patients with high values of both CEA and CA 19-9 developed p53 antibodies. In conclusion, beside classical tumor markers, circulating p53 antibodies may be considered as additional markers for the management of patients with colorectal adenocarcinomas.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::caf98f144c19310ab049cb3c4e8b7ae7Test
https://pubmed.ncbi.nlm.nih.gov/9690377Test