المؤلفون: L. Ruffier d’Epenoux, E. Tessier, A. Guillouzouic, E. Fayoux, C. Bourigault, P. Bémer, S. Corvec

المصدر: Microbiology Spectrum, Vol 10, Iss 5 (2022)

مصطلحات موضوعية: Nugent scoring, molecular test, Lactobacillus, Gardnerella vaginalis, Atopobium vaginae, bacterial vaginosis, Microbiology, QR1-502

الوصف: ABSTRACT Bacterial vaginosis (BV) is the most common cause of abnormal vaginal discharge. BV represents a dysbiosis with the acquisition of a diverse community of anaerobic bacteria and a reduction in lactobacilli burden. Our objective was to evaluate the Aptima BV assay kit for the diagnosis of BV. From May to August 2019, we enrolled outpatients and inpatients, including nonpregnant women above 18 with vaginosis symptoms, consulting at Nantes University hospital. The Aptima BV assay measures the loads of Gardnerella vaginalis, Atopobium vaginae, and Lactobacillus species in relation to overall bacterial load. The Aptima BV assay was compared to Nugent scoring (NS). A total of 456 women were enrolled, and 347 patients met the inclusion criteria with data available for the analysis. NS was used to classify the samples and 144 (41.5%) samples were classified as normal (NS = 0–3), 45 (13%) as BV (NS = 7–10), 38 (11%) presented an intermediate vaginal microbiota (3

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2165-0497Test

الوصول الحر: https://doaj.org/article/1fb5752772684972bbe151c2edd90253Test

المؤلفون: P. Bémer, C. Bourigault, A. Jolivet-Gougeon, C. Plouzeau-Jayle, C. Lemarie, R. Chenouard, A. Valentin, S. Bourdon, A. Leroy, S. Corvec

المصدر: Journal of Bone and Joint Infection, Vol 5, Pp 89-95 (2020)

مصطلحات موضوعية: Orthopedic surgery, RD701-811

الوصف: Introduction: The diagnosis of prosthetic joint infections (PJIs) can be difficult in the chronic stage and is based on clinical and paraclinical evidence. A minimally invasive serological test against the main pathogens encountered during PJI would distinguish PJI from mechanical loosening.Methods: We performed a prospective, multicentre, cross-sectional study to assess the contribution of serology in the diagnosis of PJI. Over a 2-year period, all patients undergoing prosthesis revision were included in the study. A C-reactive protein assay and a serological test specifically designed against 5 bacterial species (Staphylococcus aureus, S. epidermidis, S. lugdunensis, Streptococcus agalactiae, Cutibacterium acnes) were performed preoperatively. Five samples per patient were taken intraoperatively during surgery. The diagnosis of PJI was based on clinical and bacteriological criteria according to guidelines.Results: Between November 2015 and November 2017, 115 patients were included, 49 for a chronic PJI and 66 for a mechanical problem. Among patients with PJI, a sinus tract was observed in 32.6% and a C-reactive protein level ≥10 mg/L in 74.5%. The PJI was monomicrobial in 43 cases (targeted staphylococci, 24; S. agalactiae, 1; C. acnes, 2; others, 16), and polymicrobial in 6 cases (12.2%). Sensitivity, specificity, positive predictive value and negative predictive value were 75.0%, 82.1%, 58.3% and 90.8%, respectively, for targeted staphylococci. Specificity/negative predictive value was 97.3%/100% for S. agalactiae and 83.8% /96.9% for C. acnes.Conclusions: The serological tests are insufficient to affirm the diagnosis of PJI for the targeted bacteria. Nevertheless, the excellent NPV may help clinicians to exclude PJI.

وصف الملف: electronic resource

العلاقة: https://jbji.copernicus.org/articles/5/89/2020/jbji-5-89-2020.pdfTest; https://doaj.org/toc/2206-3552Test

الوصول الحر: https://doaj.org/article/64bf1d02d302446f85a8300f1951cbfbTest

المؤلفون: C, Bouchand, C, Bourigault, F, Le Gallou, S, Corvec, A, Andréo, D, Lepelletier

المصدر: Journal of Hospital Infection. 131:126-128

مصطلحات موضوعية: Microbiology (medical), Infectious Diseases, General Medicine

الوصف: Extended-spectrum β-lactamase-producing Enterobacterales (E-ESBL) are commensal multidrug-resistant (MDR) bacteria of the digestive tract whose prevalence has risen sharply worldwide and in Europe over the past two decades.To assess digestive carriage at hospital readmission of a large cohort of 2509 patients with E-ESBL carriage over a five-year survey; 833 (33%) patients were readmitted at least once.A retrospective, single-centre survey conducted at a tertiary care hospital in France.Among patients with several hospital readmissions (range: 2-13), the proportion of patients still E-ESBL-colonized at hospital readmission, detected by systematic screening for E-ESBL colonization, was80% within an 18-month period after prior hospitalization with the first E-ESBL isolation.There is a need to reconsider the continuation of systematic screening for E-ESBL colonization because of a high rate of patients still colonized at hospital readmission over a long period of time.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::076bcfd3fc300acba0be95d14da6710cTest
https://doi.org/10.1016/j.jhin.2022.10.004Test

المؤلفون: Pascale Bémer, C. Bourigault, Anne Jolivet-Gougeon, Sandra Bourdon, Rachel Chenouard, Stéphane Corvec, Carole Lemarié, Anne-Sophie Valentin, Anne-Gaëlle Leroy, Chloé Plouzeau-Jayle

المصدر: Journal of Bone and Joint Infection, Vol 5, Pp 89-95 (2020)
Journal of Bone and Joint Infection

مصطلحات موضوعية: medicine.medical_specialty, Prosthetic joint, medicine.medical_treatment, specificity, medicine.disease_cause, Prosthesis, Serology, 03 medical and health sciences, 0302 clinical medicine, lcsh:Orthopedic surgery, Internal medicine, Medicine, anti-staphylococcal antibodies, Orthopedics and Sports Medicine, Multiplex, negative predictive value, 030212 general & internal medicine, prosthetic joint infection, 030222 orthopedics, Chronic stage, business.industry, Prosthetic joint infection, sensitivity, lcsh:RD701-811, Infectious Diseases, Streptococcus agalactiae, Staphylococcus aureus, Surgery, business, serological diagnosis, Research Paper

الوصف: Introduction: The diagnosis of prosthetic joint infections (PJIs) can be difficult in the chronic stage and is based on clinical and paraclinical evidence. A minimally invasive serological test against the main pathogens encountered during PJI would distinguish PJI from mechanical loosening.Methods: We performed a prospective, multicentre, cross-sectional study to assess the contribution of serology in the diagnosis of PJI. Over a 2-year period, all patients undergoing prosthesis revision were included in the study. A C-reactive protein assay and a serological test specifically designed against 5 bacterial species (Staphylococcus aureus, S. epidermidis, S. lugdunensis, Streptococcus agalactiae, Cutibacterium acnes) were performed preoperatively. Five samples per patient were taken intraoperatively during surgery. The diagnosis of PJI was based on clinical and bacteriological criteria according to guidelines.Results: Between November 2015 and November 2017, 115 patients were included, 49 for a chronic PJI and 66 for a mechanical problem. Among patients with PJI, a sinus tract was observed in 32.6% and a C-reactive protein level ≥10 mg/L in 74.5%. The PJI was monomicrobial in 43 cases (targeted staphylococci, 24; S. agalactiae, 1; C. acnes, 2; others, 16), and polymicrobial in 6 cases (12.2%). Sensitivity, specificity, positive predictive value and negative predictive value were 75.0%, 82.1%, 58.3% and 90.8%, respectively, for targeted staphylococci. Specificity/negative predictive value was 97.3%/100% for S. agalactiae and 83.8% /96.9% for C. acnes.Conclusions: The serological tests are insufficient to affirm the diagnosis of PJI for the targeted bacteria. Nevertheless, the excellent NPV may help clinicians to exclude PJI.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d72045d7737de1ae1fb894820addc7e7Test
https://jbji.copernicus.org/articles/5/89/2020/jbji-5-89-2020.pdfTest

المؤلفون: C. Ferriot, C. Bouchand, A. Andreo, Didier Lepelletier, A. Rahal, F. Le Gallou, Aurélie Guillouzouic, C. Gras-Leguen, S. Corvec, C. Flamant, Elise Launay, C. Bourigault

المصدر: The Journal of hospital infection. 116

مصطلحات موضوعية: Microbiology (medical), Cross Infection, Neonatal intensive care unit, biology, business.industry, Enterobacter cloacae complex, Enterobacteriaceae Infections, Infant, Newborn, Outbreak, Mothers, General Medicine, biology.organism_classification, Microbiology, Disease Outbreaks, Electrophoresis, Gel, Pulsed-Field, Infectious Diseases, Intensive Care Units, Neonatal, Enterobacter cloacae, Medicine, Humans, Female, business

الوصف: Summary We report the investigation to control an Enterobacter cloacae complex outbreak in a neonatal intensive care unit from November 2020 to February 2021. Pulsed-field gel electrophoresis showed that five of eight cases were infected with a clonal strain. Breast pumps, shared among mothers in the unit, could have contributed to the spread of the clonal spread.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4ab99ba886d6a9d3a2852eb4aebd35dTest
https://pubmed.ncbi.nlm.nih.gov/34774974Test

المؤلفون: Didier Lepelletier, Gabriel Birgand, C. Bourigault, Serge Aho, Jean-Christophe Lucet, Cyril Ong

المساهمون: Service d'épidémiologie et d'hygiène hospitalières (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Microbiotes, Hôtes, Antibiotiques et Résistances bactériennes (MiHAR) (MiHAR), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)

المصدر: Journal of Hospital Infection
Journal of Hospital Infection, WB Saunders, 2020, 106, pp.332-334. ⟨10.1016/j.jhin.2020.08.008⟩

مصطلحات موضوعية: Microbiology (medical), Staphylococcus aureus, medicine.medical_specialty, [SDV]Life Sciences [q-bio], Descriptive survey, Mupirocin, Nose, 030501 epidemiology, medicine.disease_cause, 03 medical and health sciences, chemistry.chemical_compound, Surveys and Questionnaires, medicine, Humans, Surgical Wound Infection, Infection control, Orthopedic Procedures, Povidone-Iodine, Decontamination, Qualitative Research, Infection Control, 0303 health sciences, 030306 microbiology, business.industry, General surgery, Chlorhexidine, General Medicine, Staphylococcal Infections, Thoracic Surgical Procedures, Anti-Bacterial Agents, 3. Good health, Infectious Diseases, chemistry, Carrier State, Anti-Infective Agents, Local, France, 0305 other medical science, business, Decolonization, medicine.drug

الوصف: Summary The objective was to describe French hospital nasal screening and decolonization procedures before clean surgery procedures. Information for participants was sent to the French Society for Infection Control members in June 2018. Seventy hospitals participated in the survey; 40% (N = 28) declared having institutional decolonization procedures: 64% (N = 18) in orthopaedic and 56% (N = 15) in cardiac surgeries. All hospitals used mupirocin for nasal decolonization and body decolonization with chlorhexidine (N = 16) or povidone iodine (N = 10). This study is the first to be performed in France giving information in this field. Screening/decolonization procedures are heterogeneous and the evaluation of their clinical impact remains complex.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::973680c8306500fde51bcbd86d42abe1Test
https://doi.org/10.1016/j.jhin.2020.08.008Test

المؤلفون: Didier Lepelletier, V. Bompas, M. Quimbre, C. Bourigault, C. Bouchand, F. Le Gallou, C. Ferriot, A. Rahal, A-C. Guille des Buttes, N. Bodet, A. Andreo

المصدر: The Journal of hospital infection. 114

مصطلحات موضوعية: Microbiology (medical), medicine.medical_specialty, business.industry, General Medicine, Surgery, Infectious Diseases, Text mining, Ambulatory Surgical Procedures, Risk Factors, Outpatients, Medicine, Humans, Surgical Wound Infection, business, Surgical site infection

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb08e4a8e52b667c7e7cf005f7a33805Test
https://pubmed.ncbi.nlm.nih.gov/33901581Test

المؤلفون: Charlotte Mascart, Guillaume Chapelet, Pascale Bémer, C. Bourigault, Eric Batard, Laure de Decker, Anne Sophie Boureau, Emmanuel Montassier, Gilles Berrut, Raphaële Bachtarzi

المصدر: Infection and Drug Resistance. 12:1763-1770

مصطلحات موضوعية: Pharmacology, medicine.medical_specialty, biology, business.industry, Psychoactive drug, Urine, Antimicrobial, biology.organism_classification, Enterobacteriaceae, Infectious Diseases, Carriage, Antibiotic resistance, Internal medicine, Medicine, Pharmacology (medical), Colonization, Medical prescription, business, medicine.drug

الوصف: Background The worldwide dissemination of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae has become a major health concern. Previous studies have shown that psychoactive drugs have intrinsic antimicrobial activity and may play a role in the dissemination of antibiotic resistance. The objective of this study was to assess the association between prescriptions for psychoactive drug and urine colonization with ESBL-producing Enterobacteriaceae. Subjects Ninety-five patients were included; 19 cases (urine colonization with an ESBL-producing Enterobacteriaceae) and 76 controls (urine colonization with non ESBL-producing Enterobacteriaceae); and were matched for age and gender. Methods A retrospective 1:4 matched case-control study design was used. All patients colonized with an Enterobacteriaceae isolate in Nantes University Hospital from March to November 2014, were screened before inclusion in the study. Prescriptions data for psychoactive drugs were collected from the electronic medical records. Univariate and multivariate conditional logistic regression analyses were performed. Results Thirty-seven patients (38.9%) were treated with psychoactive drugs, of whom 10 (52.6%) were in the ESBL-producing group and 27 (35.5%) were in the non-ESBL group. Mean (SD) age was 71.2 (23.1) years. In multivariate analyses, previous antimicrobial therapy within 6 months (OR=7.12, 95% CI 1.15-44.18; p=0.035) and previous colonization with an ESBL-producing organism (OR=44.87, 95% CI 1.26-1594.19; p=0.037) were associated with urine colonization with ESBL-producing Enterobacteriaceae. Conclusions Our findings revealed that a history of previous antimicrobial therapy and previous colonization with ESBL-producing organisms are important risk factors in an elderly population. Psychoactive drugs were not associated with urinary carriage of ESBL-producing Enterobacteriaceae. Further studies are required to explore the relationship between psychoactive drugs and colonization with ESBL-producing Enterobacteriaceae.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::7f1c275f70a8d8ddfb875e4af7918290Test
https://doi.org/10.2147/idr.s200029Test

المؤلفون: M.-E. Juvin, S. Corvec, Didier Lepelletier, S. Evain, C. Bourigault

المساهمون: Microbiotes, Hôtes, Antibiotiques et Résistances bactériennes (MiHAR) (MiHAR), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)

المصدر: Journal of Hospital Infection
Journal of Hospital Infection, WB Saunders, 2019, 102, pp.25-30. ⟨10.1016/j.jhin.2019.02.002⟩

مصطلحات موضوعية: Adult, Male, Microbiology (medical), medicine.medical_specialty, medicine.drug_class, [SDV]Life Sciences [q-bio], viruses, Antibiotics, 030501 epidemiology, Patient Readmission, 03 medical and health sciences, 0302 clinical medicine, stomatognathic system, Surveys and Questionnaires, Internal medicine, Epidemiology, Prevalence, medicine, Humans, Colonization, 030212 general & internal medicine, Aged, Retrospective Studies, Aged, 80 and over, Hospital readmission, urogenital system, business.industry, Enterobacteriaceae Infections, Antibiotic exposure, Outbreak, General Medicine, Middle Aged, biochemical phenomena, metabolism, and nutrition, Anti-Bacterial Agents, 3. Good health, Carbapenem-Resistant Enterobacteriaceae, Infectious Diseases, Carriage, Carrier State, Cohort, Female, 0305 other medical science, business

الوصف: Colonization by carbapenemase-producing Enterobacteriaceae (CPE) may persist for several months after hospital discharge, especially in patients with altered microbiota.To identify how many previously OXA-48 CPE-positive patients identified during an outbreak period were readmitted; to evaluate their CPE-positive or -negative digestive tract colonization at hospital readmission and during readmission stay; and to assess the role of antibiotic exposure on their CPE colonization status during readmission.All CPE cohort patients from June 2013 to May 2016 (N = 189) were registered in a survey database and were systematically identified at readmission by a daily informatics and alert program using specific hospital population number. Each cohort patient was systematically screened for CPE colonization on the day of readmission and then weekly if the length of stay was more than six days.In all, 114 (60.3%) patients previously CPE-colonized were readmitted to our hospital. Excluding the 12 patients who were not screened because their period of readmission was24 h, 88 patients were negative (86.3%) and 14 were positive (13.7%) for CPE colonization at first hospital readmission. The 14 CPE-positive patients did not change their infectious status and remained CPE-positive during the study period. Of the 88 negative patients, 65 remained negative during the study period, and 23 subsequently became CPE-positive after the negative readmission screening. CPE-positive colonization was significantly associated with antibiotic exposure during readmission periods (P0.001).Negative screens at hospital readmission did not necessarily predict resolution of CPE carriage. Antibiotic exposure appears to influence the risk of remaining CPE positive.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::746024e8c9b28499be2accf0d44acb9aTest
https://doi.org/10.1016/j.jhin.2019.02.002Test

المؤلفون: N. Ferronnière, Jérôme Gournay, C. Bourigault, M. Le Rhun, Nicolas Musquer, Didier Lepelletier, Gabriel Birgand, M.-E. Juvin, S. Corvec, N. Bodet, S. Wiesel, F. Le Gallou

المصدر: Journal of Hospital Infection. 99:422-426

مصطلحات موضوعية: Adult, Male, Microbiology (medical), Carbapenemase-Producing Enterobacteriaceae, medicine.medical_specialty, Endoscope, Gastrointestinal Diseases, Klebsiella pneumoniae, Carbapenem-resistant enterobacteriaceae, Gastroenterology, Disease Outbreaks, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Disease Transmission, Infectious, medicine, Humans, 030212 general & internal medicine, Duodenoscopy, Aged, Aged, 80 and over, medicine.diagnostic_test, biology, business.industry, Transmission (medicine), Outbreak, General Medicine, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Klebsiella Infections, Endoscopy, Carbapenem-Resistant Enterobacteriaceae, Infectious Diseases, Female, 030211 gastroenterology & hepatology, business

الوصف: Carbapenemase-producing Klebsiella pneumoniae (OXA-48 CPE) were identified in five patients who underwent an endoscopy with the same duodenoscope in October 2015. The endoscope was the only epidemiological link between these cases. A transient contamination of the duodenoscope following a failure in the disinfection process may have been the cause of transmission.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25c0b5e96f3eb2e047a145b9eddfa95eTest
https://doi.org/10.1016/j.jhin.2018.04.015Test