المؤلفون: Attwood K, Fleyshman D, Prendergast L, Paszkiewicz G, Omilian AR, Bshara W, Gurova K

المصدر: Breast Cancer: Targets and Therapy, Vol Volume 9, Pp 301-311 (2017)

مصطلحات موضوعية: SSRP1, SPT16, breast cancer, predictive marker, prognosis, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Kristopher Attwood,1 Daria Fleyshman,2 Laura Prendergast,2 Geraldine Paszkiewicz,2 Angela R Omilian,3 Wiam Bshara,3 Katerina Gurova2 1Department of Biostatistics and Bioinformatics, 2Department of Cell Stress Biology, 3Department of Pathology, Roswell Park Cancer Institute, Buffalo, NY, USA Abstract: Understanding the underlying reasons for tumor aggressiveness, such as why some tumors grow slowly and locally, while others rapidly progress to a lethal metastatic disease, is still limited. This is especially critical in breast cancer (BrCa) due to its high prevalence and also due to the possibility that it can be detected early. Several oncogenes and tumor suppressors have been identified and are used in the prognosis and treatment of BrCa. However, even with these markers, the outcome within BrCa subtypes is highly variable. Chromatin organization has long been acknowledged as a factor that plays an important role in tumor progression, but molecular mechanisms defining chromatin dynamics are largely missing. We have recently found that histone chaperone FACT (facilitates chromatin transcription) is overexpressed in ~18–20% of BrCa cases. FACT is elevated upon transformation of mammary epithelial cells and is essential for viability of tumor cells. BrCa cells with high FACT have a more aggressive transcriptional program than those with low FACT cells. Based on this we propose that FACT may be a marker of aggressive BrCa. In this study, we aimed to comprehensively characterize the pattern of FACT expression in BrCa in relation to other molecular and clinical prognostic markers. We developed and tested an assay for the detection and quantitation of protein levels of both FACT subunits, SSRP1, and SPT16, in clinical samples. We compared the value of mRNA and protein as potential markers of disease aggressiveness using a large cohort of patients (n=1092). We demonstrated that only SSRP1 immunohistochemical staining is a reliable indicator of FACT levels in tumor samples. High SSRP1 correlated with known markers of poor prognosis, such as negative hormone receptor status, presence of Her2, high-grade tumors, and tumors of later clinical stage. At the same time, no strong correlation between SSRP1 expression and survival was detected when all samples were analyzed together. Clear trend toward longer survival of patients with low or no SSRP1 expression in tumor samples was seen in several subgroups of patients, and most importantly significant association of high SSRP1 expression with shorter disease-free survival was detected in patients with early-stage and low-grade BrCa, the category of patients with the highest demand in predictive marker of disease progression. Keywords: SSRP1, SPT16, breast cancer, predictive marker, prognosis

وصف الملف: electronic resource

العلاقة: https://www.dovepress.com/prognostic-value-of-histone-chaperone-fact-subunits-expression-in-brea-peer-reviewed-article-BCTTTest; https://doaj.org/toc/1179-1314Test

الوصول الحر: https://doaj.org/article/7ab33489db6d4ef887c0aa924367b8ceTest

المؤلفون: Albain, K., Darke, A., Mack, P., Redman, M., Cheng, T., Moon, J., Holland, W., Borczuk, A., Chay, C., Morris, P., Vallieres, E., Kratzke, R., Molina, J., Kolesar, J., Chen, Y., Macrae, R., Matsumoto, S., Reid, M., Zirpoli, G., Davis, W., Ondracek, R., Bshara, W., Omilian, A., Gandara, D., Kelly, K., Santella, R., Ambrosone, C.

المصدر: Journal of Thoracic Oncology ; volume 13, issue 10, page S333 ; ISSN 1556-0864

مصطلحات موضوعية: Pulmonary and Respiratory Medicine, Oncology

الإتاحة: https://doi.org/10.1016/j.jtho.2018.08.263Test
https://api.elsevier.com/content/article/PII:S1556086418312218?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S1556086418312218?httpAccept=text/plainTest

المؤلفون: Bianchi-Smiraglia, A, Wawrzyniak, J A, Bagati, A, Marvin, E K, Ackroyd, J, Moparthy, S, Bshara, W, Fink, E E, Foley, C E, Morozevich, G E, Berman, A E, Shewach, D S, Nikiforov, M A

المصدر: Cell Death & Differentiation ; volume 22, issue 11, page 1858-1864 ; ISSN 1350-9047 1476-5403

مصطلحات موضوعية: Cell Biology, Molecular Biology

الإتاحة: https://doi.org/10.1038/cdd.2015.47Test
http://www.nature.com/articles/cdd201547.pdfTest
http://www.nature.com/articles/cdd201547Test

المؤلفون: Köbel, M, Madore, J, Ramus, S J, Clarke, B A, Pharoah, P D P, Deen, S, Bowtell, D D, Odunsi, K, Menon, U, Morrison, C, Lele, S, Bshara, W, Sucheston, L, Beckmann, M W, Hein, A, Thiel, F C, Hartmann, A, Wachter, D L, Anglesio, M S, Høgdall, E, Jensen, A, Høgdall, C, Kalli, K R, Fridley, B L, Keeney, G L, Fogarty, Z C, Vierkant, R A, Liu, S, Cho, S, Nelson, G, Ghatage, P, Gentry-Maharaj, A, Gayther, S A, Benjamin, E, Widschwendter, M, Intermaggio, M P, Rosen, B, Bernardini, M Q, Mackay, H, Oza, A, Shaw, P, Jimenez-Linan, M, Driver, K E, Alsop, J, Mack, M, Koziak, J M, Steed, H, Ewanowich, C, DeFazio, A, Chenevix-Trench, G

المصدر: British Journal of Cancer ; volume 111, issue 12, page 2297-2307 ; ISSN 0007-0920 1532-1827

مصطلحات موضوعية: Cancer Research, Oncology

الإتاحة: https://doi.org/10.1038/bjc.2014.567Test
http://www.nature.com/articles/bjc2014567.pdfTest
http://www.nature.com/articles/bjc2014567Test

المؤلفون: Chinnam, M., Wang, Y., Zhang, X., Gold, D. L., Khoury, T., Nikitin, A. Y., Foster, B. A., Li, Y., Bshara, W., Morrison, C. D., Payne Ondracek, R. D., Mohler, J. L., Goodrich, D. W.

المصدر: JNCI Journal of the National Cancer Institute ; volume 106, issue 11, page dju306-dju306 ; ISSN 0027-8874 1460-2105

مصطلحات موضوعية: Cancer Research, Oncology

الإتاحة: https://doi.org/10.1093/jnci/dju306Test
http://academic.oup.com/jnci/article-pdf/106/11/dju306/17313409/dju306.pdfTest

المؤلفون: Francis, J, Smiley, S, Battiwalla, M, Wetzler, M, Barcos, M, Bshara, W, Paplham, P, Brown, K, Syta, M, Lamonica, D, Loud, P, McCarthy, P

المصدر: Bone Marrow Transplantation ; volume 44, issue 12, page 827-828 ; ISSN 0268-3369 1476-5365

مصطلحات موضوعية: Transplantation, Hematology

الإتاحة: https://doi.org/10.1038/bmt.2009.183Test
https://www.nature.com/articles/bmt2009183.pdfTest
https://www.nature.com/articles/bmt2009183Test

المؤلفون: Khoury, T, Zirpoli, G, Cohen, S.M, Geradts, J, Omilian, A, Davis, W, Bshara, W, Miller, R, Mathews, M.M, Troester, M, Palmer, J.R, Ambrosone, C.B

المصدر: American Journal of Clinical Pathology, 148(2)

مصطلحات موضوعية: major clinical study, antigen expression, estrogen receptor, priority journal, Tissue Array Analysis, middle aged, human, biological marker, correlation analysis, cancer classification, diagnostic test accuracy study, data base, tumor cell, intraductal carcinoma, full section, tissue fixation, breast cancer, sensitivity and specificity, epidermal growth factor receptor 2, tissue microarray, immunohistochemistry, adult, tumor marker, medical society, procedures, staining, female, diagnostic accuracy, breast biopsy, Biomarkers

الوصف: Objectives Ki-67 has been proposed to be used as a surrogate marker to differentiate luminal breast carcinomas (BCs). The purpose of this study was to determine the utility of and best approaches for using tissue microarrays (TMAs) and Ki-67 staining to distinguish luminal subtypes in large epidemiology studies of luminal/human epidermal growth factor receptor 2 (HER2)-negative BC. Methods Full-section and TMA (three 0.6-mm cores and two 1.0-mm cores) slides of 109 cases were stained with Ki-67 antibody. We assessed two ways of collapsing TMA cores: a weighted approach and mitotically active approach. Results For cases with at least a single 0.6-mm TMA core (n = 107), 16% were misclassified using a mitotically active approach and 11% using a weighted approach. For cases with at least a single 1.0-mm TMA core (n = 101), 5% were misclassified using either approach. For the 0.6-mm core group, there were 33.3% discordant cases. The number of discordant cases increased from 18% in the group of two cores to 40% in the group of three cores (P =.039). Conclusions Ki-67 tumor heterogeneity was common in luminal/HER2- BC. Using a weighted approach was better than using a mitotically active approach for core to case collapsing. At least a single 1.0-mm core or three 0.6-mm cores are required when designing a study using TMA.

العلاقة: https://doi.org/10.17615/s59a-5b63Test; https://cdr.lib.unc.edu/downloads/tx31qv971?file=thumbnailTest; https://cdr.lib.unc.edu/downloads/tx31qv971Test

الإتاحة: https://doi.org/10.17615/s59a-5b63Test
https://cdr.lib.unc.edu/downloads/tx31qv971?file=thumbnailTest
https://cdr.lib.unc.edu/downloads/tx31qv971Test

المؤلفون: Bianchi-Smiraglia, A, Bagati, A, Fink, E E, Moparthy, S, Wawrzyniak, J A, Marvin, E K, Battaglia, S, Jowdy, P, Kolesnikova, M, Foley, C E, Berman, A E, Kozlova, N I, Lipchick, B C, Paul-Rosner, L M, Bshara, W, Ackroyd, J J, Shewach, D S, Nikiforov, M A

المصدر: Oncogene ; volume 36, issue 1, page 84-96 ; ISSN 0950-9232 1476-5594

مصطلحات موضوعية: Cancer Research, Genetics, Molecular Biology

الإتاحة: https://doi.org/10.1038/onc.2016.178Test
https://www.nature.com/articles/onc2016178.pdfTest
https://www.nature.com/articles/onc2016178Test

المؤلفون: Dubil, E.A., Bshara, W., Odunsi, K.O., Morrison, C., Tian, C., Risinger, J.I., Maxwell, G.L., Hamilton, C.A., Darcy, K.M., Consortium, W.

المصدر: Gynecologic Oncology ; volume 133, page 114-115 ; ISSN 0090-8258

مصطلحات موضوعية: Obstetrics and Gynecology, Oncology

الإتاحة: https://doi.org/10.1016/j.ygyno.2014.03.302Test
https://api.elsevier.com/content/article/PII:S0090825814005381?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0090825814005381?httpAccept=text/plainTest

المؤلفون: Byrd, K.A., Maxwell, G.L., Bshara, W., Risinger, J.I., Tian, C., Omilian, A.R., Odunsi, K.O., Morrison, C., Conrads, T.P., Consortium, W.

المصدر: Gynecologic Oncology ; volume 133, page 93-94 ; ISSN 0090-8258

مصطلحات موضوعية: Obstetrics and Gynecology, Oncology

الإتاحة: https://doi.org/10.1016/j.ygyno.2014.03.249Test
https://api.elsevier.com/content/article/PII:S0090825814004855?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0090825814004855?httpAccept=text/plainTest