المؤلفون: Bryce Lim, Katrin Domsch, Moritz Mall, Ingrid Lohmann

المصدر: Molecular Systems Biology, Vol 20, Iss 3, Pp 144-161 (2024)

مصطلحات موضوعية: Alternative Fate Repression, Cell Identity, Cell Fate Plasticity, Epigenetic Silencing, Transcriptional Repressor, Biology (General), QH301-705.5, Medicine (General), R5-920

الوصف: Abstract Precision in the establishment and maintenance of cellular identities is crucial for the development of multicellular organisms and requires tight regulation of gene expression. While extensive research has focused on understanding cell type-specific gene activation, the complex mechanisms underlying the transcriptional repression of alternative fates are not fully understood. Here, we provide an overview of the repressive mechanisms involved in cell fate regulation. We discuss the molecular machinery responsible for suppressing alternative fates and highlight the crucial role of sequence-specific transcription factors (TFs) in this process. Depletion of these TFs can result in unwanted gene expression and increased cellular plasticity. We suggest that these TFs recruit cell type-specific repressive complexes to their cis-regulatory elements, enabling them to modulate chromatin accessibility in a context-dependent manner. This modulation effectively suppresses master regulators of alternative fate programs and their downstream targets. The modularity and dynamic behavior of these repressive complexes enables a limited number of repressors to canalize and maintain major and minor cell fate decisions at different stages of development.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1744-4292Test

الوصول الحر: https://doaj.org/article/4c8d1ce2da324abd8138a54f285ee6a3Test

المؤلفون: Lewis A. Fraser, Andrew B. Kinghorn, Marco S. L. Tang, Yee-Wai Cheung, Bryce Lim, Shaolin Liang, Roderick M. Dirkzwager, Julian A. Tanner

المصدر: Molecules, Vol 20, Iss 12, Pp 21298-21312 (2015)

مصطلحات موضوعية: aptamer, SELEX, microbead, monoclonal bead, ePCR, FACS, Organic chemistry, QD241-441

الوصف: The functionalisation of microbeads with oligonucleotides has become an indispensable technique for high-throughput aptamer selection in SELEX protocols. In addition to simplifying the separation of binding and non-binding aptamer candidates, microbeads have facilitated the integration of other technologies such as emulsion PCR (ePCR) and Fluorescence Activated Cell Sorting (FACS) to high-throughput selection techniques. Within these systems, monoclonal aptamer microbeads can be individually generated and assayed to assess aptamer candidate fitness thereby helping eliminate stochastic effects which are common to classical SELEX techniques. Such techniques have given rise to aptamers with 1000 times greater binding affinities when compared to traditional SELEX. Another emerging technique is Fluorescence Activated Droplet Sorting (FADS) whereby selection does not rely on binding capture allowing evolution of a greater diversity of aptamer properties such as fluorescence or enzymatic activity. Within this review we explore examples and applications of oligonucleotide functionalised microbeads in aptamer selection and reflect upon new opportunities arising for aptamer science.

وصف الملف: electronic resource

العلاقة: http://www.mdpi.com/1420-3049/20/12/19766Test; https://doaj.org/toc/1420-3049Test

الوصول الحر: https://doaj.org/article/b1ccaa4f090c49ba86e40cc0e412673fTest

المؤلفون: Lintang Wahyu Charisa Raharjo, Mike Natanael Norend Mandagie, Ariel Angelo Nanlohy, Jeremy Bryce Lim, Aldilla Ranita Purba

المصدر: JURNAL HUKUM, POLITIK DAN ILMU SOSIAL; Vol. 2 No. 1 (2023): MARET : JURNAL HUKUM, POLITIK DAN ILMU SOSIAL; 103-112 ; 2963-7651 ; 2963-8704 ; 10.55606/jhpis.v2i1

مصطلحات موضوعية: Pengungsi, Jerman, Uni Eropa, Identitas, Problematika

الوصف: Perang yang terjadi antara dua negara maupun lebih akan mengakibatkan terjadinya pengungsian secara besar guna mencari kehidupan yang lebih layak dan melarikan dari wilayah konflik. Negara-negara anggota Uni Eropa merupakan negara yang mau membuka perbatasan dan menerima pengungsi dari wilayah konflik terutama dari wilayah Timur Tengah. Jerman dengan kebijakan pintu terbuka yang dicetuskan pada masa kepemimpinan Angela Merkel meenrima banyak apresiasi dari banyak pihak. Akan tetapi dampak negative muncul dengan membeludaknya angka kriminalitas di Jerman dan hal ini membuat Jerman secara tidak langsung memikirkan keputusan mereka dalam menerima pengungsi. Artikel ini akan membahas problematika yang muncul dari penerimaan pengungsi bagi Jerman dan bagaimana identitas masyarakat Eropa memiliki peranan yang cukup penting.

وصف الملف: application/pdf

العلاقة: https://ejurnal.politeknikpratama.ac.id/index.php/jhpis/article/view/1025/1010Test; https://ejurnal.politeknikpratama.ac.id/index.php/jhpis/article/view/1025Test

الإتاحة: https://doi.org/10.55606/jhpis.v2i1.1025Test
https://doi.org/10.55606/jhpis.v2i1Test
https://ejurnal.politeknikpratama.ac.id/index.php/jhpis/article/view/1025Test

المؤلفون: Bettina Weigel, Jana F. Tegethoff, Sarah D. Grieder, Bryce Lim, Bhuvaneswari Nagarajan, Yu-Chao Liu, Jule Truberg, Dimitris Papageorgiou, Juan M. Adrian-Segarra, Laura K. Schmidt, Janina Kaspar, Eric Poisel, Elisa Heinzelmann, Manu Saraswat, Marleen Christ, Christian Arnold, Ignacio L. Ibarra, Joaquin Campos, Jeroen Krijgsveld, Hannah Monyer, Judith B. Zaugg, Claudio Acuna, Moritz Mall

المصدر: Molecular Psychiatry.

مصطلحات موضوعية: Cellular and Molecular Neuroscience, Psychiatry and Mental health, Molecular Biology

الوصف: MYT1L is an autism spectrum disorder (ASD)-associated transcription factor that is expressed in virtually all neurons throughout life. How MYT1L mutations cause neurological phenotypes and whether they can be targeted remains enigmatic. Here, we examine the effects of MYT1L deficiency in human neurons and mice. Mutant mice exhibit neurodevelopmental delays with thinner cortices, behavioural phenotypes, and gene expression changes that resemble those of ASD patients. MYT1L target genes, including WNT and NOTCH, are activated upon MYT1L depletion and their chemical inhibition can rescue delayed neurogenesis in vitro. MYT1L deficiency also causes upregulation of the main cardiac sodium channel, SCN5A, and neuronal hyperactivity, which could be restored by shRNA-mediated knockdown of SCN5A or MYT1L overexpression in postmitotic neurons. Acute application of the sodium channel blocker, lamotrigine, also rescued electrophysiological defects in vitro and behaviour phenotypes in vivo. Hence, MYT1L mutation causes both developmental and postmitotic neurological defects. However, acute intervention can normalise resulting electrophysiological and behavioural phenotypes in adulthood.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::883ef535719f02352bc496a78c75f176Test
https://doi.org/10.1038/s41380-023-01959-7Test

المؤلفون: Julian A. Tanner, S Liang, Joseph Manitta, Jack S. Richards, AB Kinghorn, Simon Chi-Chin Shiu, Yee-Wai Cheung, Lewis A. Fraser, Marco S. L. Tang, Bryce Lim, Roderick M. Dirkzwager, Dean Andrew

المصدر: Biosensors and Bioelectronics. 100:591-596

مصطلحات موضوعية: Models, Molecular, Aptamer, Plasmodium falciparum, Microfluidics, Biomedical Engineering, Biophysics, Nanotechnology, Biosensing Techniques, macromolecular substances, 02 engineering and technology, Microfluidic biosensor, 01 natural sciences, Limit of Detection, Electrochemistry, medicine, Humans, 3d print, L-Lactate Dehydrogenase, biology, Chemistry, 010401 analytical chemistry, technology, industry, and agriculture, General Medicine, Aptamers, Nucleotide, Microfluidic Analytical Techniques, 021001 nanoscience & nanotechnology, biology.organism_classification, medicine.disease, Malaria, 0104 chemical sciences, Diagnosis of malaria, Printing, Three-Dimensional, Colorimetry, 0210 nano-technology, Biosensor, Biotechnology

الوصف: There is a critical need for better biosensors for the detection and diagnosis of malaria. We previously developed a DNA aptamer that recognises the Plasmodium falciparum lactate dehydrogenase (PfLDH) enzyme with high sensitivity and specificity. The aptamer was integrated into an Aptamer-Tethered Enzyme Capture (APTEC) assay as a laboratory-based diagnostic approach. However, a portable equipment-free point-of-care aptamer-mediated biosensor could have a significant impact on malaria diagnosis in endemic regions. Here, we present a new concept for a malaria biosensor whereby aptamers are coated onto magnetic microbeads for magnet-guided capture, wash and detection of the biomarker. A biosensor incorporating three separate microfluidic chambers was designed to enable such magnet-guided equipment-free colorimetric detection of PfLDH. A series of microfluidic biosensor prototypes were optimised to lower rates of inter-chamber diffusion, increase sensitivity, and provide a method for point-of-care sample testing. The biosensor showed high sensitivity and specificity when detecting PfLDH using both in vitro cultured parasite samples and using clinical samples from malaria patients. The high performance of the biosensor provides a proof-of-principle for a portable biosensor that could be adaptable for a variety of aptamer-mediated diagnostic scenarios.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2930c8860f5124bd51eed00ebb9c519eTest
https://doi.org/10.1016/j.bios.2017.10.001Test

المؤلفون: Julian A. Tanner, Bryce Lim, Lewis A. Fraser, Roderick M. Dirkzwager, Marco S. L. Tang, S Liang, Yee-Wai Cheung, AB Kinghorn

المصدر: Molecules
Molecules, Vol 20, Iss 12, Pp 21298-21312 (2015)

مصطلحات موضوعية: ePCR, Aptamer, FACS, Oligonucleotides, Pharmaceutical Science, Nanotechnology, Review, Biology, microbead, Analytical Chemistry, lcsh:QD241-441, Fluorescence-Activated Cell Sorting, lcsh:Organic chemistry, Drug Discovery, High-Throughput Screening Assays, Humans, Physical and Theoretical Chemistry, Selection (genetic algorithm), SELEX, Oligonucleotide, SELEX Aptamer Technique, Organic Chemistry, aptamer, monoclonal bead, Microbead (research), Aptamers, Nucleotide, Microspheres, Chemistry (miscellaneous), Molecular Medicine, Systematic evolution of ligands by exponential enrichment

الوصف: The functionalisation of microbeads with oligonucleotides has become an indispensable technique for high-throughput aptamer selection in SELEX protocols. In addition to simplifying the separation of binding and non-binding aptamer candidates, microbeads have facilitated the integration of other technologies such as emulsion PCR (ePCR) and Fluorescence Activated Cell Sorting (FACS) to high-throughput selection techniques. Within these systems, monoclonal aptamer microbeads can be individually generated and assayed to assess aptamer candidate fitness thereby helping eliminate stochastic effects which are common to classical SELEX techniques. Such techniques have given rise to aptamers with 1000 times greater binding affinities when compared to traditional SELEX. Another emerging technique is Fluorescence Activated Droplet Sorting (FADS) whereby selection does not rely on binding capture allowing evolution of a greater diversity of aptamer properties such as fluorescence or enzymatic activity. Within this review we explore examples and applications of oligonucleotide functionalised microbeads in aptamer selection and reflect upon new opportunities arising for aptamer science.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c70fee9c4760cdae49521886725df071Test
https://doi.org/10.3390/molecules201219766Test

المؤلفون: Fraser, Lewis A., Kinghorn, Andrew B., Tang, Marco S. L., Yee-Wai Cheung, Bryce Lim, Shaolin Liang, Dirkzwager, Roderick M., Tanner, Julian A.

المصدر: Molecules; Dec2015, Vol. 20 Issue 12, p21298-21312, 15p, 5 Diagrams

مصطلحات موضوعية: OLIGONUCLEOTIDE analysis, ENZYME analysis, CELL analysis, APTAMERS, EMULSIONS, MATHEMATICAL models

مستخلص: The functionalisation of microbeads with oligonucleotides has become an indispensable technique for high-throughput aptamer selection in SELEX protocols. In addition to simplifying the separation of binding and non-binding aptamer candidates, microbeads have facilitated the integration of other technologies such as emulsion PCR (ePCR) and Fluorescence Activated Cell Sorting (FACS) to high-throughput selection techniques. Within these systems, monoclonal aptamer microbeads can be individually generated and assayed to assess aptamer candidate fitness thereby helping eliminate stochastic effects which are common to classical SELEX techniques. Such techniques have given rise to aptamers with 1000 times greater binding affinities when compared to traditional SELEX. Another emerging technique is Fluorescence Activated Droplet Sorting (FADS) whereby selection does not rely on binding capture allowing evolution of a greater diversity of aptamer properties such as fluorescence or enzymatic activity. Within this review we explore examples and applications of oligonucleotide functionalised microbeads in aptamer selection and reflect upon new opportunities arising for aptamer science. [ABSTRACT FROM AUTHOR]

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