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1دورية أكاديمية
المؤلفون: Jean, WC, Britz, GW, DiMeco, F, Elmi-Terander, A, McIntyre, C
المصدر: Neurosurgical focus. 51(2):E1
مصطلحات موضوعية: Medicin och hälsovetenskap
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2دورية أكاديمية
المؤلفون: Wang, Z, Yang, W, Britz, GW, Lombard, FW, Warner, DS, Sheng, H
مصطلحات موضوعية: Aorta, Thoracic, Spinal Cord, Neurons, Animals, Mice, Inbred C57BL, Spinal Cord Ischemia, Disease Models, Animal, Thoracotomy, Rotarod Performance Test, Random Allocation, Surgical Instruments, Cell Death, Regional Blood Flow, Locomotion, Time Factors, Male
الوصف: Use of genetically manipulated mice facilitates understanding pathological mechanisms in many diseases and contributes to therapy development. However, there is no practical and clinically relevant mouse model available for spinal cord ischemia. This report introduces a simplified long-term outcome mouse model of spinal cord ischemia. Male C57Bl/6J mice were anesthetized with isoflurane and endotracheally intubated. The middle segment of the thoracic aorta was clamped for 0, 8, 10 or 12 min via left lateral thoracotomy. Rectal temperature was maintained at 37.0+/-0.5 degrees C. A laser Doppler probe was used to measure lumbar spinal cord blood flow during thoracic aorta cross-clamping. Open field locomotor function and rotarod performance were evaluated at 1h and 1, 3, 5, and 7 days post-injury. Surviving neurons in the lumbar ventral horn were counted at 7 days post-injury. Cross-clamping the middle segment of the thoracic aorta resulted in approximately 90% blood flow reduction in the lumbar spinal cord. Neurological deficit and neuronal cell death were associated with ischemia duration. Another set of mice were subjected to 10 min aortic clamping or sham surgery and neurological function was examined at 1h and 1, 3, 5, 7, 14, and 28 days. Four of 5 mice (80%) in the injured group survived 28 days and had significant neurological deficit. This study indicates that cross-clamping of the aorta via left thoracotomy is a simple and reliable method to induce spinal cord ischemia in mice allowing definition of long-term outcome.
وصف الملف: application/pdf
العلاقة: Journal of neuroscience methods; S0165-0270(10)00189-5; https://hdl.handle.net/10161/23297Test
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3دورية أكاديمية
المؤلفون: Tenenbein, PK, Godhke, B, Britz, GW, Sekhar, LN, Lam, AM
المصدر: Journal of Neurosurgical Anesthesiology ; volume 18, issue 4, page 304 ; ISSN 0898-4921
الإتاحة: https://doi.org/10.1097/00008506-200610000-00076Test
http://journals.lww.com/00008506-200610000-00076Test -
4دورية أكاديمية
المؤلفون: Guinn NR, McDonagh DL, Borel CO, Wright DR, Zomorodi AR, Powers CJ, Warner DS, Lam AM, Britz GW
المصدر: Journal of Neurosurgical Anesthesiology; 2011 Jan, Vol. 23 Issue 1, p35-40, 6p
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5دورية أكاديمية
المؤلفون: Rozet I, Tontisirin N, Muangman S, Vavilala MS, Souter MJ, Lee LA, Kincaid MS, Britz GW, Lam AM
المصدر: Anesthesiology; 2007 Nov, Vol. 107 Issue 5, p697-704, 8p
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6دورية أكاديمية
المؤلفون: Ngai AC, Nguyen T, Meno JR, Britz GW, Ngai, Al C, Nguyen, Thien-Son, Meno, Joseph R, Britz, Gavin W
المصدر: Stroke (00392499); 2007 Jan, Vol. 38 Issue 1, p124-130, 7p
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7دورية أكاديمية
المؤلفون: Sviri GE, Newell DW, Lewis DH, Douville C, Ghodke B, Chowdhary M, Lam AM, Haynor D, Zaaroor M, Britz GW, Sviri, Gill E, Newell, David W, Lewis, David H, Douville, Colleen, Ghodke, Basavaraj, Chowdhary, Minku, Lam, Arthur M, Haynor, David, Zaaroor, Menashe, Britz, Gavin W
المصدر: Stroke (00392499); 2006 Nov, Vol. 37 Issue 11, p2738-2743, 6p
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8دورية أكاديمية