المؤلفون: Welsh J. A., Goberdhan D. C. I., O'Driscoll L., Buzas E. I., Blenkiron C., Bussolati B., Cai H., Di Vizio D., Driedonks T. A. P., Erdbrugger U., Falcon-Perez J. M., Fu Q. -L., Hill A. F., Lenassi M., Lim S. K., Mahoney M. y. G., Mohanty S., Moller A., Nieuwland R., Ochiya T., Sahoo S., Torrecilhas A. C., Zheng L., Zijlstra A., Abuelreich S., Bagabas R., Bergese P., Bridges E. M., Brucale M., Burger D., Carney R. P., Cocucci E., Crescitelli R., Hanser E., Harris A. L., Haughey N. J., Hendrix A., Ivanov A. R., Jovanovic-Talisman T., Kruh-Garcia N. A., Faustino V. K. -L., Kyburz D., Lasser C., Lennon K. M., Lotvall J., Maddox A. L., Martens-Uzunova E. S., Mizenko R. R., Newman L. A., Ridolfi A., Rohde E., Rojalin T., Rowland A., Saftics A., Sandau U. S., Saugstad J. A., Shekari F., Swift S., Ter-Ovanesyan D., Tosar J. P., Useckaite Z., Valle F., Varga Z., van der Pol E., van Herwijnen M. J. C., Wauben M. H. M., Wehman A. M., Williams S., Zendrini A., Zimmerman A. J., Thery C., Witwer K. W., Ahmad S., Ahmed D. A. K., Ahmed S. H., Aikawa E., Akbar N., Akiyoshi K., Al-Adra D. P., Al-Masawa M. E., Albanese M., Alberro A., Alcaraz M. J., Alexander-Brett J., Alexander K. L., Ali N., Alibhai F. J., Allelein S., Allenby M. C., Almeida F., de Almeida L. P., Almousa S. W., Altan-Bonnet N., Altei W. F., Alvarez-Llamas G., Alvarez C. L., An H. J., Anand K., Andaloussi S. E. L., Anderson J. D., Andriantsitohaina R., Ansari K. I., Anselmo A., Antoniou A., Aqil F., Arab T., Archer F., Arif S., Armstrong D. A., Arntz O. J., Arsene P., Arteaga-Blanco L., Asokan N., Aspelin T., Atkin-Smith G. K., Aubert D., Ayyar K. K., Azlan M., Azoidis I., Becot A., Bach J. -M., Bachurski D., Bae S., Bagge R. O., Baj-Krzyworzeka M., Balaj L., Balbi C., van Balkom B. W. M., Ballal A. R., Bano A., Banzet S., Bare Y., Barile L., Barman B., Barranco I., Barreca V., Bart G., Barteneva N. S., Basso M., Batish M., Bauer N. R., Baxter A. A., Bazie W. W., Bazzan E., Beaumont J. E. J., Bebawy M., Bebelman M. P., Bedina-Zavec A., Beetler D. J., Beke-Somfai T., Belleannee C., Benedikter B. J., Benediktsdottir B. E., Berardi A. C., Bergamelli M., Bertolini I., Bhattacharyya A., Bhattacharyya S. N., Biller S. J., Billottet C., Bissler J. J., Blanc-Brude O., Blijdorp C. J., Bobis-Wozowicz S., Bodart-Santos V., Bodnar B. R., Boilard E., Boireau W., Bokun V., Bollard S. M., Bollini S., Bongiovanni A., Bongiovanni L., Bonifay A., Boppart M. D., Borras F. E., Bosch S., Boselli D., Bottini M., Bouffard J., Boulanger C. M., Boutros P. C., Boyadjian O., Boysen A. T., Bozkurt B. T., Bramich K. P., Braun F., Del Carmen Bravo-Miana R., Breakefield X. O., Brenna S., Brennan K., Brennan M. A., Breyne K., Brigstock D. R., Brisson A. R., Brodie C., Bruno K. A., Bucci C., Buch S., Buck A., Bukva M., Bulte J. W. M., Buratta S., Burgy O., Burnier J. V., Burrows K., Busatto S., Buzas K., Byrd J. B., Caceres-Verschae A., Caires H. R., Campos-Silva C., Camussi G., de Candia P., Carceller C., Fernandez-Becerra C., Carrasco A. G. M., Carter D. R. F., Cavallaro S., Cavallero S., Cerda-Troncoso C., Chahwan R., Chalupska R., Chamley L. W., Chandra P. K., Chang W. -W., Charest A., Chen C., Chen H., Chen Q., Chen S., Chen Y., Cheng L., Chernyshev V. S., Chetty V. K., Chitti S. V., Cho S. -G., Cho Y. -K., Choi B. H., Chutipongtanate S., Cicardi M. E., Cifuentes-Rius A., Ciullo A., Clayton A., Cleary J. A., Cocozza F., Coffey R. J., Collino F., Colombo F., Colosetti P., Compan-Bertomeu A., Constanzo J., Corbeil D., Cordeiro-da-Silva A., Costa J., Couch Y., Courageux Y., Coutant K., Coyle B., Cretich M., Cronemberger-Andrade A., Crossland R. E., Cucher M. A., Czystowska-Kuzmicz M., D'Acunzo P., D'Agnano I., D'Agostino V. G., D'Arrigo D., D'Souza-Schorey C., Dagur R. S., Danielson K. M., Das S., Dauphin T., Davidson S. M., Davies O. G., Davies R. L., Davis C. N., Deep G., Degosserie J., Van Delen M., Deliwala V., Dellar E. R., Van Deun J., Dev A., Deville S., Devitt A., Dhondt B., Dieterich L. C., Dittmer D. P., Dobosh B., Dobra G., Dogra N., Dohi E., Dolo V., Domashevich T. V., Dominici M., Dong L., Dore E., Dragovic R. A., Drittanti L., Droste M., Duan W., Durmaz E., Dutta S., Eguchi T., Eichenberger R. M., Eitan E., Ekstrom K., Eldh M., Elie-Caille C., Enciso-Martinez A., Esmaeili R., Ettelaie C., Forsonits A. I., Fabbri M., Falasca M., Fan H., Fatima F., Fazeli A., Fernandez-Rhodes M., Fernandez-Prada C., Ferraro M. J., Ferreira J., Ferreira R. F., Figueroa-Hall L. K., Figueroa-Valdes A. I., Fioretti P. V., Flenady S., Flores-Bellver M., Fok E. K., Fonseka P., Forbes K., Ford V. J., Fornaguera C., Forte D., Forte S., Fortunato O., Franklin J. L., Freitas D., Frelet-Barrand A., Fujita Y., Gartner K., Gorgens A., Gabriel A. M., Gabrielli M., Gabrielsson S., Galinsoga A., Galisova A., Gamage T. K. J. B., Gao Y., Garcia-Contreras M., Garcia Garcia M. M., Garcia M. N., Gargiulo E., Garibotti H. G. -K., Mc Gee M. M., Genard G. C., Geraci F., Ghanam J., Ghatak S., Ghavami M., Ghebosu R. E., Gho Y. S., Ghosal S., Giamas G., Giebel B., Gilbert C., Gimona M., Girao H., Giusti I., Gizzie E. A., Glamoclija S., Glass S. E., Gobbo J., Godbole N., Goetz J. G., Gololobova O., Gomez-Florit M., Goncalves J. P., Gorgun C., Gori A., Gorska S., Graner M. W., Grau G. E., Grech L., Greening D. W., Gross R. M., Gross J. C., Gruber J., Gualerzi A., Guanzon D., Gudbergsson J. M., Guerin C. L., Guerra F., Guillen M. I., Gujar V., Guo W., Gupta V. B., Gupta V. K., Gustafson D., Gyukity-Sebestyen E., Holker P., Hade M. D., Hagey D. W., Han C., Han P., Hanayama R., Handberg A., Harada M., Harmati M., Harrison P., Harrison R. A., Haynes P. A., He M., Hegyesi H., Hisey C. L., Hochberg F. H., Nolte-'tHoen E. N. M. N., Holcar M., Holder B., Holnthoner W., Holthofer H., Hooper D. C., Hosseini-Beheshti E., Hosseinkhani B., Howard J., Howe K. L., Hoyle N. R., Hrdy J., Hu G., Huang Y., Huber V., Hudoklin S., Hufnagel A., Hulett M. D., Hunt S., Hyenne V., Di Ianni E., Iannotta D., Ibrahim A. G. E., Ibrahim S. A., Ikezu S., Ikezu T., Im H., Inal J. M., Inic-Kanada A., Inngjerdingen M., Inoshima Y., Ivanova A., Izquierdo E., Jorgensen M. M., Jackson H. K., Jacobsen S., Jadue F., Javeed N., Jay S. M., Jayachandran M., Jayasinghe M. K., Jenster G., Jeppesen D. K., Jeronimo C., Jiang L., Jin J., Jingushi K., Jo D. -G., Joerger-Messerli M. S., Jones J. C., Jones M. K., de Jong O. G., Ferrante A. W., Coleman L. G., Juncker D., Jung S., Jurek B., Jurga M., Justilien V., Kabani M., Kalluri R., Kamali-Moghaddam M., Kanada M., Kang T., Kano S. -I., Kaparakis-Liaskos M., Karnas E., Karoichan A., Kashanchi F., Kashani S. A., Kashyap N. N., Katsur M., Kau-Strebinger S., Kauffman A. C., Kaur S., Kehoe O., Kelwick R. J. R., Kenari A. N., Kestecher B. M., Keulers T. G., Van Keuren-Jensen K. R., Khalaj K., Khamari D., Khanabdali R., Khomyakova E., Khoo A., Kim D. H., Kim D., Kim H. S., Kim I. -S., Kim S., Kim Y., Kima P. E., Kislinger T., Klingeborn M., Knight R., Komuro H., Koncz A., Konstantinou T., van der Koog L., Kooijmans S. A. A., Kornek M. T., Kosanovic M., Kostallari E., Koukoulis T. F., Kourembanas S., Krameralbers E. -M., Kralj-Iglic V., Krasemann S., Krasnodembskaya A. D., Krawczynska N. J., Kreft M. E., Kuehn M. J., Kuipers M. E., Kulaj K., Kuligowski J., Kumagai Y., Kumar A., Kumar S., Kumari M., Kundrotas G., Kurochkin I. V., Kuroda M., Kurzawa-Akanbi M., Kweskin S. J., Lazaro-Ibanez E., Lorincz A., Lai A., Lai C. P., Laitinen S., Landreville S., Lange S., Langevin S. M., Langlois M. -A., Languino L. R., Lannigan J., Lark D. S., Larregina A. T., Laurent L. C., Laurin D., Lavieu G., Lawson C., Le Lay S., Leandro K., Ledreux A., Lee C., Lee D. -S., Lee H., Lee S. Y., Lee T. R., Lee W. -L., Lefterov I., Lei X., Leivo J., Lemaire Q., Leme A. F. P., Lemon S. M., Lenzini S., Leor J., Levy E., Li B., Li G., Li J. J., Li Q., Li X., Liang X., Lim R., Limongi T., Line A., Lins P. P., Lippens L., Liu G., Llorente A., Longjohn M. N., van de Loo F. A. J., Lorenowicz M. J., Lorico A., Loudig O., Loyer X., Lozano-Andres E., Lu B., Lu Q., Lubart Q., Lucien F., Lunavat T. R., Lundberg L. E., Lundy D. J., Luoto J. C., Lyden D. C., Muller J. A., Macphee D. J., Madec E., Magana S. M., Mahairaki V., Malhi H., Malnou C. E., Mamand D. R., Man K., Manno M., Mantel P. -Y., Marangon T., Marban E., Marcilla A., Maremanda K. P., Margolis L., Marinas-Pardo L., Maric I., Martin S. S., Martinez-Martinez E., Martel C., Martin-Duque P., Martin-Jaular L., Martinez-Murillo P. A., Martinez-Pacheco S., Martins-Marques T., Mary B., Marzan A. L., Matamoros-Angles A., Mathivanan S., Matsuzaki J., Mayan M. D., Mazzeo C., Mbengue M., McCann M. J., McIlvenna L. C., McVey M. J., Meisner-Kober N., Mellergaard M., Melli G., Menck K., Menjivar N. G., Menon R., Mentkowski K. I., Miklavcic J. J., Miklosi A. G., Milutinovic B., Minciacchi V. R., Mirzaei M., Mishra S., Mitchell M. I., Mladenovic D., Mohamadi E., Momen-Heravi F., Mondal S. K., Monguio-Tortajada M., Moon J., Morandi M. I., Moreau V., Moreira L. R., Morelli A. E., Mori M. A., Morimoto M., Mosser M., Motaung T. E., Moussay E., Mugoni V., Mullier F., Muraca M., Murugesan S., Musante L., Musico A., Nemeth A., Nemeth K., Nadeau A., Nam G. -H., Naora H., Natoli R., Nawaz M., Nazarenko I., Ndukaife J. C., Nedeva C., Nejsum P., Nelissen I., Neri C., Neri T., Neviani P., Ng C. Y., van Niel G., Nikiforova N., Nimrichter L., Nitin C., Njock M. -S., Noel D., Noghero A., Nolan J. P., Noppen S., Hooten N. N., da Silva Novaes A., O'loghlen A., Oesterreicher J., Oh S. W., Olah A., Olivier M., Ong S. L., Ortiz A., Ortiz L. A., Ostergaard O., Osorio O. A., Osteikoetxea X., Ostrowski M., Otaegui D., Otahal A., Ozawa P. M. M., Ozkocak D. C., Paloczi K., Perez-Gonzalez R., Pachane B. C., Padinharayil H., Paget D., Paggetti J., Palacio P. C. L., Pallasch C. P., Palmulli R., Pang B., Paniushkina L., Pantazi P., Paolini L., Papademetrio D. L., Parisse P., Park D. J., Park J., Park Y. -G., Patton J. G., Peake N. J., Pegtel D. M., Peinado H., Perut F., Pfaffl M. W., Pfeiffer A., Phan T. K., Phinney D. G., Phylactou L. A., Picciolini S., Pietrowska M., Piffoux M., Pinheiro C., Pink R. C., Pleet M. L., Pocsfalvi G., Poh Q. H., Poojary G., Poon I. K. H., Poppa G., Del Portillo H. A., Pospichalova V., Potter S., Powell B. H., Powis S. J., Prada I., Prasadam I., Preusser C., Pua H. H., Pucci F., Puhm F., Puig B., Pulliam L., Purnianto A., Puutio J. M. M., Quilang R. C., Rabbani P. S., Rackov G., Radeghieri A., Radu C. M., Raffai R. L., Raghav A., Rahbari M., Rahman M. D. M., Rahman M. M., Rai A. J., Raimondo S., Raju S., Rak J., Ramaswamy L., Ramirezricardo J., Ramirez M. I., Rani S., Raposo G., Rather H. A., Razim A., Reale A., Reategui E., Reddel C. J., Reddy S. K., Redenti S., Reed S. L., Regevrudzki N., Reiners K. S., Resnik N., Rice G. E., Ricklefs F. L., Rilla K., Rimmer M. P., Roballo K. C. S., Robbins P. D., Roberts D. D., Roca J., Rodal A. A., Rodrigues-Junior D. M., Rodrigues M. L., Roefs M. T., Rogers R. G., Romani R., Romano M., Rome S., Romih R., Romolo A., De Rossi T., Rouschop K. M., Routenberg D. A., Roux Q., van Royen M. E., Rozo A. J., Rufino-Ramos D., Rughetti A., Russell A. E., Rutter S. F., Rysmakhanov M. S., Sanchez C. A., Sadovsky Y., Safavi-Sohi R., Sagar R., Saidu N. E. B., Saint-Pol J., Salas-Huenuleo E., Salazar-Puerta A. I., Saleem A., Salekdeh G. H., Salomon C., Salviano-Silva A., Salybekov A. A., Samuels M., Santavanond J. P., Santoro J., Santos M., Sanwlani R., Saul M. J., Schoyen T. H., Schabussova I., Scharrig E., Schekman R., Schiavi-Tritz J., Schiffelers R. M., Schmid A. M., Schneider R., Schneider S., Schoeberlein A., Schorey J. S., Seo N., Seras-Franzoso J., Shahi S., Shatnyeva O., Shea D. F., Shelke G. V., Shetty A. K., Shiba K., Shiju T. M., Shrivastava S., Shukla S., Siljander P. R. -M., Silva A. M., Singh A. P., Singh S., Skliar M., Skog J., Sluijter J. P. G., Snyder O. L., Soekmadji C., Somaida A., Somiya M., Soroczynska K., Sotillo J., Souza-Fonseca-guimaraes F., Spada S., Spiers H. V. M., Spitzberg J. D., Srivastava A., Srivastava A. K., Stepien E. L., St-Denis-bissonnette F., Stahl P. D., Stam J., Stambouli O., Stanton B. A., Stassen F. R. M., Staufer O., Steiner L., Stepanova G., Stoka V., Stoorvogel W., von Strandmann E. P., Strunk D., Stylli S. S., Su H., Subramanian S., Sui B., Sukreet S., Sulaiman E., Sung B. H., Sunkara V., Suo Z., Svenningsen P., Swatler J., Symonds E. K. C., Szeifert V., Szigyarto I. C., Toth E. A., Tasli N. P., Tahara H., Takahashi R. -U., Takakura Y., Takikawa O., Takov K., Tang V. A., Taverna S., Tawil N., Teeuwen L., Tejedor S., Tertel T., Thakur A., Thompson-Felix T., Tian C., Tikhonov A., Tiwari S., Toh W. S., Tomes J. J., Tonoli E., Trinidad C. V., Tritten L., Trivedi R., Troyer Z., Tsamchoe M., Tscherrig V., Tsering T., Turkova K., Tutanov O. S., Ueda K., Upadhya D., Urabe F., Urbanelli L., Urzi O., Vacchi E., Vader P., Vago R., Valadi H., Valkonen S., Varas-Godoy M., Vasconcelos M. H., Vechetti I. J., Veiga S. I., Vella L. J., Velot E., Verweij F. J., Vestad B., Vinay L., Viola M., Visnovitz T., Vreeland W. N., Vukman K. V., Wade P. K., van de Wakker S. I., Walther L., Wang T., Wang X., Watson D. C., Weaver A. M., Webber J. P., Weber V., Weiss L., Weiss M. L., Weiss R., Weissleder R., Wen Y., de Wever O., Wheelock A. M., White K. E., Whitehead B., Whiteside T. L., Whitley J., Wiener Z., Van Wijnen A. J., Wiklander O. P. B., Winston C. N., Wolf M., Wolfram J., Wu L., Wu Y., Wysmolek M. E., Xander P., Xavier C. P. R., Xiao Y., Xu R., Yanez-Mo M., Yamamoto T., Yamamoto Y., Yan X., Yang L., Yang Y., Yarani R., Yea K., Yedigaryan L., Yenuganti V. R., Yerneni S. S., Yeung V., Yildizhan Y., Yin H., Yokoi A., Yoshioka Y., You Y., Yuan L. -Q., Yunga S. T., Zakeri A., Zani A., Zanoni M., Zappulli V., Zarovni N., Zarubova J., Zempleni J., Zekas V., Zhang H., Zhang Q., Zhao Z., Zhou Y., Zickler A. M., Zimmermann P., Zivkovic A. M., Zocco D., Zuba-Surma E. K., Zubair H.

المساهمون: Welsh, J. A., Goberdhan, D. C. I., O'Driscoll, L., Buzas, E. I., Blenkiron, C., Bussolati, B., Cai, H., Di Vizio, D., Driedonks, T. A. P., Erdbrugger, U., Falcon-Perez, J. M., Fu, Q. -L., Hill, A. F., Lenassi, M., Lim, S. K., Mahoney, M. y. G., Mohanty, S., Moller, A., Nieuwland, R., Ochiya, T., Sahoo, S., Torrecilhas, A. C., Zheng, L., Zijlstra, A., Abuelreich, S., Bagabas, R., Bergese, P., Bridges, E. M., Brucale, M., Burger, D., Carney, R. P., Cocucci, E., Crescitelli, R., Hanser, E., Harris, A. L., Haughey, N. J., Hendrix, A., Ivanov, A. R., Jovanovic-Talisman, T., Kruh-Garcia, N. A., Faustino, V. K. -L., Kyburz, D., Lasser, C., Lennon, K. M., Lotvall, J., Maddox, A. L., Martens-Uzunova, E. S., Mizenko, R. R., Newman, L. A., Ridolfi, A., Rohde, E., Rojalin, T., Rowland, A., Saftics, A., Sandau, U. S., Saugstad, J. A., Shekari, F., Swift, S., Ter-Ovanesyan, D., Tosar, J. P., Useckaite, Z., Valle, F., Varga, Z., van der Pol, E., van Herwijnen, M. J. C., Wauben, M. H. M., Wehman, A. M., Williams, S., Zendrini, A., Zimmerman, A. J., Thery, C., Witwer, K. W., Ahmad, S., Ahmed, D. A. K., Ahmed, S. H., Aikawa, E., Akbar, N., Akiyoshi, K., Al-Adra, D. P., Al-Masawa, M. E., Albanese, M., Alberro, A., Alcaraz, M. J., Alexander-Brett, J., Alexander, K. L., Ali, N., Alibhai, F. J., Allelein, S., Allenby, M. C., Almeida, F., de Almeida, L. P., Almousa, S. W., Altan-Bonnet, N., Altei, W. F., Alvarez-Llamas, G., Alvarez, C. L., An, H. J., Anand, K., Andaloussi, S. E. L., Anderson, J. D.

مصطلحات موضوعية: ectosome, exosome, extracellular particle, extracellular vesicle, guideline, microparticle, microvesicle, minimal information requirement, MISEV, reproducibility, rigor, standardisation

الوصف: Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

وصف الملف: ELETTRONICO

العلاقة: info:eu-repo/semantics/altIdentifier/wos/WOS:001157744000001; volume:13; journal:JOURNAL OF EXTRACELLULAR VESICLES; https://hdl.handle.net/11587/517326Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85183359756

الإتاحة: https://doi.org/10.1002/jev2.12404Test
https://hdl.handle.net/11587/517326Test

المؤلفون: Thery, C, Witwer, KW, Aikawa, E, Alcaraz, MJ, Anderson, JD, Andriantsitohaina, R, Antoniou, A, Arab, T, Archer, F, Atkin-Smith, GK, Ayre, DC, Bach, JM, Bachurski, D, Baharvand, H, Balaj, L, Baldacchino, S, Bauer, NN, Baxter, AA, Bebawy, M, Beckham, C, Zavec, AB, Benmoussa, A, Berardi, AC, Bergese, P, Bielska, E, Blenkiron, C, Bobis-Wozowicz, S, Boilard, E, Boireau, W, Bongiovanni, A, Borras, FE, Bosch, S, Boulanger, CM, Breakefield, X, Breglio, AM, Brennan, MA, Brigstock, DR, Brisson, A, Broekman, MLD, Bromberg, JF, Bryl-Gorecka, P, Buch, S, Buck, AH, Burger, D, Busatto, S, Buschmann, D, Bussolati, B, Buzas, EI, Byrd, JB, Camussi, G, Carter, DRF, Caruso, S, Chamley, LW, Chang, YT, Chen, CC, Chen, S, Cheng, L, Chin, AR, Clayton, A, Clerici, SP, Cocks, A, Cocucci, E, Coffey, RJ, Cordeiro-da-Silva, A, Couch, Y, Coumans, FAW, Coyle, B, Crescitelli, R, Criado, MF, D'Souza-Schorey, C, Das, S, Chaudhuri, AD, de Candia, P, De Santana, EF, De Wever, O, del Portillo, HA, Demaret, T, Deville, S, Devitt, A, Dhondt, B, Di Vizio, D, Dieterich, LC, Dolo, V, Rubio, APD, Dominici, M, Dourado, MR, Driedonks, TAP, Duarte, FV, Duncan, HM, Eichenberger, RM, Ekstrom, K, Andaloussi, SEL, Elie-Caille, C, Erdbrugger, U, Falcon-Perez, JM, Fatima, F, Fish, JE, Flores-Bellver, M, Forsonits, A, Frelet-Barrand, A, Fricke, F, Fuhrmann, G, Gabrielsson, S, Gamez-Valero, A, Gardiner, C, Gartner, K, Gaudin, R, Gho, YS, Giebel, B, Gilbert, C, Gimona, M, Giusti, I, Goberdhan, DCI, Gorgens, A, Gorski, SM, Greening, DW, Gross, JC, Gualerzi, A, Gupta, GN, Gustafson, D, Handberg, A, Haraszti, RA, Harrison, P, Hegyesi, H, Hendrix, A, Hill, AF, Hochberg, FH, Hoffmann, KF, Holder, B, Holthofer, H, Hosseinkhani, B, Hu, GK, Huang, YY, Huber, V, Hunt, S, Ibrahim, AGE, Ikezu, T, Inal, JM, Isin, M, Ivanova, A, Jackson, HK, Jacobsen, S, Jay, SM, Jayachandran, M, Jenster, G, Jiang, LZ, Johnson, SM, Jones, JC, Jong, A, Jovanovic-Talisman, T, Jung, S, Kalluri, R, Kano, S, Kaur, S, Kawamura, Y, Keller, ET, Khamari, D, Khomyakova, E, Khvorova, A, Kierulf, P, Kim, KP, Kislinger, T, Klingeborn, M, Klinke, DJ, Kornek, M, Kosanovic, MM, Kovacs, AF, Kramer-Albers, EM, Krasemann, S, Krause, M, Kurochkin, IV, Kusuma, GD, Kuypers, S, Laitinen, S, Langevin, SM, Languino, LR, Lannigan, J, Lasser, C, Laurent, LC, Lavieu, G, Lazaro-Ibanez, E, Le Lay, S, Lee, MS, Lee, YXF, Lemos, DS, Lenassi, M, Leszczynska, A, Li, ITS, Liao, K, Libregts, SF, Ligeti, E, Lim, R, Lim, SK, Line, A, Linnemannstons, K, Llorente, A, Lombard, CA, Lorenowicz, MJ, Lorincz, AM, Lotvall, J, Lovett, J, Lowry, MC, Loyer, X, Lu, Q, Lukomska, B, Lunavat, TR, Maas, SLN, Malhi, H, Marcilla, A, Mariani, J, Mariscal, J, Martens-Uzunova, ES, Martin-Jaular, L, Martinez, MC, Martins, VR, Mathieu, M, Mathivanan, S, Maugeri, M, McGinnis, LK, McVey, MJ, Meckes, DG, Meehan, KL, Mertens, I, Minciacchi, VR, Moller, A, Jorgensen, MM, Morales-Kastresana, A, Morhayim, J, Mullier, F, Muraca, M, Musante, L, Mussack, V, Muth, DC, Myburgh, KH, Najrana, T, Nawaz, M, Nazarenko, I, Nejsum, P, Neri, C, Neri, T, Nieuwland, R, Nimrichter, L, Nolan, JP, Nolte-'t Hoen, ENM, Noren Hooten, N, O'Driscoll, L, O'Grady, T, O'Loghlen, A, Ochiya, T, Olivier, M, Ortiz, A, Ortiz, LA, Osteikoetxea, X, Ostegaard, O, Ostrowski, M, Park, J, Pegtel, DM, Peinado, H, Perut, F, Pfaffl, MW, Phinney, DG, Pieters, BCH, Pink, RC, Pisetsky, DS, von Strandmann, EP, Polakovicova, I, Poon, IKH, Powell, BH, Prada, I, Pulliam, L, Quesenberry, P, Radeghieri, A, Raffai, RL, Raimondo, S, Rak, J, Ramirez, MI, Raposo, G, Rayyan, MS, Regev-Rudzki, N, Ricklefs, FL, Robbins, PD, Roberts, DD, Rodrigues, SC, Rohde, E, Rome, S, Rouschop, KMA, Rughetti, A, Russell, AE, Saa, P, Sahoo, S, Salas-Huenuleo, E, Sanchez, C, Saugstad, JA, Saul, MJ, Schiffelers, RM, Schneider, R, Schoyen, TH, Scott, A, Shahaj, E, Sharma, S, Shatnyeva, O, Shekari, F, Shelke, GV, Shetty, AK, Shiba, K, Siljander, PRM, Silva, AM, Skowronek, A, Snyder, OL, Soares, RP, Sodar, BW, Soekmadji, C, Sotillo, J, Stahl, PD, Stoorvogel, W, Stott, SL, Strasser, EF, Swift, S, Tahara, H, Tewari, M, Timms, K, Tiwari, S, Tixeira, R, Tkach, M, Toh, WS, Tomasini, R, Torrecilhas, AC, Tosar, JP, Toxavidis, V, Urbanelli, L, Vader, P, van Balkom, BWM, van der Grein, SG, Van Deun, J, van Herwijnen, MJC, Van Keuren-Jensen, K, van Niel, G, van Royen, ME, van Wijnen, AJ, Vasconcelos, MH, Vechetti, IJ, Veit, TD, Vella, LJ, Velot, E, Verweij, FJ, Vestad, B, Vinas, JL, Visnovitz, T, Vukman, KV, Wahlgren, J, Watson, DC, Wauben, MHM, Weaver, A, Webber, JP, Weber, V, Wehman, AM, Weiss, DJ, Welsh, JA, Wendt, S, Wheelock, AM, Wiener, Z, Witte, L, Wolfram, J, Xagorari, A, Xander, P, Xu, J, Yan, XM, Yanez-Mo, M, Yin, H, Yuana, Y, Zappulli, V, Zarubova, J, Zekas, V, Zhang, JY, Zhao, ZZ, Zheng, L, Zheutlin, AR, Zickler, AM, Zimmermann, P, Zivkovic, AM, Zocco, D, Zuba-Surma, EK

المصدر: Journal of extracellular vesicles. 7(1):1535750

مصطلحات موضوعية: Medicin och hälsovetenskap

الوصول الحر: http://kipublications.ki.se/Default.aspx?queryparsed=id:139732839Test

المؤلفون: van Solinge, T.S., Abels, E., Dusoswa, S., Verhoeff, J., Garcia-Vallejo, J., Breakefield, X., Broekman, M.

المصدر: Brain and Spine ; volume 1, page 100485 ; ISSN 2772-5294

الإتاحة: https://doi.org/10.1016/j.bas.2021.100485Test
https://api.elsevier.com/content/article/PII:S2772529421004859?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2772529421004859?httpAccept=text/plainTest

المؤلفون: van Solinge, T.S. (Thomas S.), Abels, E.R. (Erik R.), van de Haar, L.L. (Lieke L.), Hanlon, K.S. (Killian S.), Maas, S.L.N. (Sybren), Schnoor, R. (Rosalie), Vrij, J. (Jeroen), Breakefield, X. (Xandra), Broekman, M.L.D. (Marike)

المصدر: Frontiers in Molecular Biosciences vol. 7

مصطلحات موضوعية: exosomes, extracellular vesicles, glioblastoma, glioma, Rab27a, tumor microenvironment

الوصف: Introduction: Glioma cells exert influence over the tumor-microenvironment in part through the release of extracellular vesicles (EVs), membrane-enclosed structures containing proteins, lipids, and RNAs. In this study, we evaluated the function of Ras-associated protein 27a (Rab27a) in glioma and evaluated the feasibility of assessing its role in EV release in glioma cells in vitro and in vivo. Methods: Rab27a was knocked down via a short hairpin RNA (shRNA) stably expressed in mouse glioma cell line GL261, with a scrambled shRNA as control. EVs were isolated by ultracentrifugation and quantified with Nanoparticle Tracking Analysis (NTA) and Tunable Resistive Pulse Sensing (TRPS). CellTiter-Glo viability assays and cytokine arrays were used to evaluate the impact of Rab27a knockdown. GL261.shRab27a cells and GL261.shControl were implanted into the left striatum of eight mice to assess tumor growth and changes in the tumor microenvironment. Results: Knockdown of Rab27a in GL261 glioma cells decreased the release of small EVs isolated at 100,000 × g in vitro (p = 0.005), but not the release of larger EVs, isolated at 10,000 × g. GL261.shRab27a cells were less viable compared to the scramble control in vitro (p < 0.005). A significant increase in CCL2 expression in shRab27a GL261 cells was also observed (p < 0.001). However, in vivo there was no difference in tumor growth or overall survival between t

وصف الملف: application/pdf

العلاقة: http://repub.eur.nl/pub/132105Test; urn:hdl:1765/132105

الإتاحة: https://doi.org/10.3389/fmolb.2020.554649Test
http://repub.eur.nl/pub/132105Test

المؤلفون: Bearer, E. L., Breakefield, X. O., Schuback, D., Reese, T. S., LaVail, J. H.

المصدر: Proceedings of the National Academy of Sciences of the United States of America, 2000 Jul 01. 97(14), 8146-8150.

الوصول الحر: https://www.jstor.org/stable/123064Test

المؤلفون: Thery, C., Witwer, K. (Kenneth), Aikawa, E. (Elena), Alcaraz, M.J. (Maria Jose), Anderson, J.D. (Johnathon D), Andriantsitohaina, R. (Ramaroson), Antoniou, A. (Anna), Arab, T. (Tanina), Archer, F. (Fabienne), Atkin-Smith, G.K. (Georgia K), Ayre, D.C. (D Craig), Bach, J.-M. (Jean-Marie), Bachurski, D. (Daniel), Baharvand, H. (Hossein), Balaj, L. (Leonora), Baldacchino, S. (Shawn), Bauer, N.N. (Natalie N), Baxter, A.A. (Amy A), Bebawy, M. (Mary), Beckham, C. (Carla), Bedina Zavec, A. (Apolonija), Benmoussa, A. (Abderrahim), Berardi, A.C. (Anna C), Bergese, P. (Paolo), Bielska, E. (Ewa), Blenkiron, C. (Cherie), Bobis-Wozowicz, S. (Sylwia), Boilard, E. (Eric), Boireau, W. (Wilfrid), Bongiovanni, A. (Antonella), Borràs, F.E. (Francesc), Bosch, S. (Steffi), Boulanger, C.M. (Chantal), Breakefield, X. (Xandra), Breglio, A.M. (Andrew M), Brennan, M.Á. (Meadhbh Á), Brigstock, D.R. (David R), Brisson, A. (Alain), Broekman, M.L.D. (Marike), Bromberg, J.F. (Jacqueline F), Bryl-Górecka, P. (Paulina), Buch, S. (Shilpa), Buck, A.H. (Amy H), Burger, D. (Dylan), Busatto, S. (Sara), Buschmann, D. (Dominik), Bussolati, B. (Benedetta), Buzas, E. (Edit), Byrd, J.B. (James Bryan), Camussi, G. (Giovanni), Carter, D.R.F. (David RF), Caruso, S. (Sarah), Chamley, L.W. (Lawrence W), Chang, Y.-T. (Yu-Ting), Chaudhuri, A.D. (Amrita Datta), Chen, C. (Chihchen), Chen, S. (Shuai), Cheng, L. (Lesley), Chin, A.R. (Andrew R), Clayton, A. (Aled), Clerici, S.P. (Stefano P), Cocks, A. (Alex), Cocucci, E. (Emanuele), Coffey, R.J. (Robert J), Cordeiro-da-Silva, A. (Anabela), Couch, Y. (Yvonne), Coumans, F.A.W. (Frank AW), Coyle, B. (Beth), Crescitelli, R. (Rossella), Criado, M.F. (Miria Ferreira), D’Souza-Schorey, C. (Crislyn), Das, S. (Saumya), de Candia, P. (Paola), De Santana, E.F. (Eliezer F), De Wever, O. (Olivier), Del Portillo, H. (Hernando), Demaret, T. (Tanguy), Deville, S. (Sarah), Devitt, A. (Andrew), Dhondt, B. (Bert), Di Vizio, D. (Dolores), Dieterich, L.C. (Lothar C), Dolo, V. (Vincenza), Dominguez Rubio, A.P. (Ana Paula), Dominici, M. (Massimo), Dourado, M.R. (Mauricio R), Driedonks, T.A.P. (Tom AP), Duarte, F.V. (Filipe V), Duncan, H.M. (Heather M), Eichenberger, R.M. (Ramon M), Ekström, K. (Karin), EL Andaloussi, S. (Samir), Elie-Caille, C. (Celine), Erdbrügger, U. (Uta), Falcon-Perez, J.M. (Juan), Fatima, F. (Farah), Fish, J.E. (Jason E), Flores-Bellver, M. (Miguel), Försönits, A. (András), Frelet-Barrand, A. (Annie), Fricke, F. (Fabia), Fuhrmann, G. (Gregor), Gabrielsson, S. (Susanne), Gámez-Valero, A. (Ana), Gardiner, C. (Chris), Gärtner, K. (Kathrin), Gaudin, R. (Raphael), Gho, Y.S. (Yong Song), Giebel, B. (B.), Gilbert, C. (Caroline), Gimona, M. (Mario), Giusti, I. (Ilaria), Goberdhan, D.C.I. (Deborah CI), Görgens, A. (André), Gorski, S.M. (Sharon M), Greening, D.W. (David W.), Gross, J.C. (Julia Christina), Gualerzi, A. (Alice), Gupta, G.N. (Gopal N), Gustafson, D. (Dakota), Handberg, A. (Aase), Haraszti, R.A. (Reka A), Harrison, P. (Paul), Hegyesi, H. (Hargita), Hendrix, A. (An), Hill, A.F. (Andrew F), Hochberg, F.H. (Fred H), Hoffmann, K.F. (Karl F), Holder, B. (Beth), Holthofer, H. (Harry), Hosseinkhani, B. (Baharak), Hu, G. (Guoku), Huang, Y. (Yiyao), Huber, V. (Veronica), Hunt, S. (Stuart), Ibrahim, A.G.-E. (Ahmed Gamal-Eldin), Ikezu, T. (Tsuneya), Inal, J.M. (Jameel), Isin, M. (Mustafa), Ivanova, A. (Alena), Jackson, H.K. (Hannah K), Jacobsen, S. (Soren), Jay, S.M. (Steven M), Jayachandran, M. (Muthuvel), Jenster, G.W. (Guido), Jiang, L. (Lanzhou), Johnson, S.M. (Suzanne M), Jones, J.C. (Jennifer C), Jong, A. (Ambrose), Jovanovic-Talisman, T. (Tijana), Jung, S. (Stephanie), Kalluri, R. (Raghu), Kano, S.-I. (Shin-ichi), Kaur, S. (Sukhbir), Kawamura, Y. (Yumi), Keller, E.T. (Evan T), Khamari, D. (Delaram), Khomyakova, E. (Elena), Khvorova, A. (Anastasia), Kierulf, P. (Peter), Kim, K.P. (Kwang Pyo), Kislinger, T. (Thomas), Klingeborn, M. (Mikael), Klinke, D.J. (David J), Kornek, M. (Miroslaw), Kosanović, M.M. (Maja M), Kovács, Á.F. (Árpád Ferenc), Krämer-Albers, E.-M. (Eva-Maria), Krasemann, S. (Susanne), Krause, M. (Mirja), Kurochkin, I.V. (Igor V), Kusuma, G.D. (Gina D), Kuypers, S. (Sören), Laitinen, S. (Saara), Langevin, S.M. (Scott M), Languino, L.R. (Lucia R), Lannigan, J. (Joanne), Lässer, C. (Cecilia), Laurent, L.C. (Louise C), Lavieu, G. (Gregory), Lázaro-Ibáñez, E. (Elisa), Le Lay, S. (Soazig), Lee, M.-S. (Myung-Shin), Lee, Y.X.F. (Yi Xin Fiona), Lemos, D.S. (Debora S), Lenassi, M. (Metka), Leszczynska, A. (Aleksandra), Li, I.T.S. (Isaac TS), Liao, K. (Ke), Libregts, S.F. (Sten), Ligeti, E. (Erzsebet), Lim, R. (Rebecca), Lim, S.K. (Sai Kiang), Linē, A. (Aija), Linnemannstöns, K. (Karen), Llorente, A. (Alicia), Lombard, C.A. (Catherine A), Lorenowicz, M.J. (Magdalena J), Lörincz, Á.M. (Ákos M), Lötvall, J. (Jan), Lovett, J. (Jason), Lowry, M.C. (Michelle C), Loyer, X. (Xavier), Lu, Q. (Quan), Lukomska, B. (Barbara), Lunavat, T.R. (Taral R), Maas, S.L.N. (Sybren), Malhi, H. (Harmeet), Marcilla, A. (Antonio), Mariani, J. (Jacopo), Mariscal, J. (Javier), Martens-Uzunova, E.S. (Elena), Martin-Jaular, L. (Lorena), Martinez, M.C. (M Carmen), Martins, V.R. (Vilma Regina), Mathieu, M. (Mathilde), Mathivanan, S. (Suresh), Maugeri, M. (Marco), McGinnis, L.K. (Lynda K), McVey, M.J. (Mark J), Meckes, D.G. (David G), Meehan, K.L. (Katie L), Mertens, I. (Inge), Minciacchi, V.R. (Valentina R), Möller, A. (Andreas), Møller Jørgensen, M. (Malene), Morales-Kastresana, A. (Aizea), Morhayim, J. (Jess), Mullier, F. (Francois), Muraca, M. (Maurizio), Musante, L. (Luca), Mussack, V. (Veronika), Muth, D.C. (Dillon C), Myburgh, K.H. (Kathryn H), Najrana, T. (Tanbir), Nawaz, M. (Muhammad), Nazarenko, I. (Irina), Nejsum, P. (Peter), Neri, C. (Christian), Neri, T. (Tommaso), Nieuwland, C.C.M. (Carolien) van, Nimrichter, L. (Leonardo), Nolan, J.P. (John P), Nolte-’t Hoen, E.N.M. (Esther NM), Hooten, N.N. (Nicole Noren), O’Driscoll, L. (Lorraine), O’Grady, T. (Tina), O’Loghlen, A. (Ana), Ochiya, T. (Takahiro), Olivier, M. (Martin), Ortiz, A. (Alberto), Ortiz, L.A. (Luis A), Osteikoetxea, X. (Xabier), Ostegaard, O. (Ole), Ostrowski, M. (Matias), Park, J. (Jaesung), Pegtel, D.M. (D. Michiel), Peinado, H. (Hector), Perut, F. (Francesca), Pfaffl, M.W. (Michael W), Phinney, D.G. (Donald G), Pieters, B.C.H. (Bartijn CH), Pink, R.C. (Ryan C), Pisetsky, D.S. (David S), Pogge von Strandmann, E. (Elke), Polakovicova, I. (Iva), Poon, I.K.H. (Ivan KH), Powell, B.H. (Bonita H), Prada, I. (Ilaria), Pulliam, L. (Lynn), Quesenberry, P. (Peter), Radeghieri, A. (Annalisa), Raffai, R.L. (Robert L), Raimondo, S. (Stefania), Rak, J. (Janusz), Ramirez, M.I. (Marcel I.), Raposo, L. (Luís), Rayyan, M.S. (Morsi S), Regev-Rudzki, N. (Neta), Ricklefs, F.L. (Franz L), Robbins, P.D. (Paul D), Roberts, D.D. (David D), Rodrigues, S.C. (Silvia C), Rohde, E. (Eva), Rome, S. (Sophie), Rouschop, K.M.A. (Kasper MA), Rughetti, A. (Aurelia), Russell, A.E. (Ashley E), Saá, P. (Paula), Sahoo, S. (Susmita), Salas-Huenuleo, E. (Edison), Sánchez, C. (Catherine), Saugstad, J.A. (Julie A), Saul, M.J. (Meike J), Schiffelers, R.M. (Raymond), Schneider, R. (Raphael), Schøyen, T.H. (Tine Hiorth), Scott, A. (Aaron), Shahaj, E. (Eriomina), Sharma, S. (Shivani), Shatnyeva, O. (Olga), Shekari, F. (Faezeh), Shelke, G.V. (Ganesh Vilas), Shetty, A.K. (Ashok K), Shiba, K. (Kiyotaka), Siljander, P. (Pia), Silva, A.M. (Andreia M), Skowronek, A. (Agata), Snyder, O.L. (Orman L), Soares, R.P. (Rodrigo Pedro), Sódar, B.W. (Barbara W), Soekmadji, C. (Carolina), Sotillo, J. (Javier), Stahl, P.D. (Philip D), Stoorvogel, W. (Willem), Stott, S.L. (Shannon L), Strasser, E.F. (Erwin F), Swift, S. (Simon), Tahara, H. (Hidetoshi), Tewari, M. (Muneesh), Timms, K. (Kate), Tiwari, S. (Swasti), Tixeira, R. (Rochelle), Tkach, M. (Mercedes), Toh, W.S. (Wei Seong), Tomasini, R. (Richard), Torrecilhas, A.C. (Ana Claudia), Tosar, J.P. (Juan Pablo), Toxavidis, V. (Vasilis), Urbanelli, L. (Lorena), Vader, P. (Pieter), Balkom, B.W.M. (Bas) van, van der Grein, S.G. (Susanne G), Van Deun, J. (Jan), van Herwijnen, M.J.C. (Martijn JC), Van Keuren-Jensen, K. (Kendall), van Niel, G. (Guillaume), Royen, M.E. (Martin), van Wijnen, A.J. (Andre J), Vasconcelos, M.H. (M Helena), Vechetti, I.J. (Ivan J), Veit, T.D. (Tiago D), Vella, L.J. (Laura J.), Velot, É. (Émilie), Verweij, F.J. (Frederik J), Vestad, B. (Beate), Viñas, J.L. (Jose L), Visnovitz, T. (Tamás), Vukman, K.V. (Krisztina V), Wahlgren, J. (Jessica), Watson, D.C. (Dionysios C), Wauben, M.H.M. (Marca), Weaver, A. (Alissa), Webber, J.P. (Jason P), Weber, V. (Viktoria), Wehman, A.M. (Ann M), Weiss, D.J. (Daniel J), Welsh, J.A. (Joshua A), Wendt, S. (Sebastian), Wheelock, A.M. (Asa M), Wiener, Z. (Zoltán), Witte, L. (Leonie), Wolfram, J. (Joy), Xagorari, A. (Angeliki), Xander, P. (Patricia), Xu, J. (Jing), Yan, X. (Xiaomei), Yáñez-Mó, M. (María), Yin, H. (Hang), Yuana, Y., Zappulli, V. (Valentina), Zarubova, J. (Jana), Žėkas, V. (Vytautas), Zhang, J.-Y. (Jian-ye), Zhao, Z. (Zezhou), Zheng, L. (Lei), Zheutlin, A.R. (Alexander R), Zickler, A.M. (Antje M), Zimmermann, P. (Pascale), Zivkovic, A.M. (Angela M), Zocco, D. (Davide), Zuba-Surma, E.K. (Ewa K)

المصدر: Journal of Extracellular Vesicles vol. 8 no. 1

مصطلحات موضوعية: ectosomes, exosomes, extracellular vesicles, guidelines, microparticles, microvesicles, minimal information requirements, reproducibility, rigor, standardization

الوصف: The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make the

وصف الملف: application/pdf

العلاقة: http://repub.eur.nl/pub/112565Test; urn:hdl:1765/112565

الإتاحة: https://doi.org/10.1080/20013078.2018.1535750Test
http://repub.eur.nl/pub/112565Test

المؤلفون: Gustafsson, G., Lööv, C., Persson, E., Lázaro, D., Takeda, S., Bergström, J., Erlandsson, A., Sehlin, D., Balaj, L., György, B., Hallbeck, M., Outeiro, T., Breakefield, X., Hyman, B., Ingelsson, M.

المصدر: Cellular and Molecular Neurobiology

الوصف: In Parkinson’s disease and other Lewy body disorders, the propagation of pathology has been accredited to the spreading of extracellular α-synuclein (α-syn). Although the pathogenic mechanisms are not fully understood, cell-to-cell transfer of α-syn via exosomes and other extracellular vesicles (EVs) has been reported. Here, we investigated whether altered molecular properties of α-syn can influence the distribution and secretion of α-syn in human neuroblastoma cells. Different α-syn variants, including α-syn:hemi-Venus and disease-causing mutants, were overexpressed and EVs were isolated from the conditioned medium. Of the secreted α-syn, 0.1–2% was associated with vesicles. The major part of EV α-syn was attached to the outer membrane of vesicles, whereas a smaller fraction was found in their lumen. For α-syn expressed with N-terminal hemi-Venus, the relative levels associated with EVs were higher than for WT α-syn. Moreover, such EV-associated α-syn:hemi-Venus species were internalized in recipient cells to a higher degree than the corresponding free-floating forms. Among the disease-causing mutants, A53T α-syn displayed an increased association with EVs. Taken together, our data suggest that α-syn species with presumably lost physiological functions or altered aggregation properties may shift the cellular processing towards vesicular secretion. Our findings thus lend further support to the tenet that EVs can mediate spreading of harmful α-syn species and thereby contribute to the pathology in α-synucleinopathies.

وصف الملف: application/pdf

العلاقة: http://hdl.handle.net/21.11116/0000-0002-8736-9Test; http://hdl.handle.net/21.11116/0000-0002-8738-7Test

الإتاحة: https://doi.org/10.1007/s10571-018-0622-5Test
http://hdl.handle.net/21.11116/0000-0002-8736-9Test
http://hdl.handle.net/21.11116/0000-0002-8738-7Test

المؤلفون: Mathivanan, S., Maugeri, M., McGinnis, L. K., McVey, M. J., Meckes, D. G., Meehan, K. L., Mertens, I., Minciacchi, V. R., Moller, A., Moller Jorgensen, M., Morales-Kastresana, A., Morhayim, J., Mullier, F., Muraca, M., Musante, L., Mussack, V., Muth, D. C., Myburgh, K. H., Najrana, T., NAWAZ, M., Nazarenko, I., Nejsum, P., Neri, C., Neri, T., Nieuwland, R., Nimrichter, L., Nolan, J. P., Nolte-'t Hoen, E. N., Noren Hooten, N., O'Driscoll, L., O'Grady, T., O'Loghlen, A., Ochiya, T., Olivier, M., Ortiz, A., Ortiz, L. A., Osteikoetxea, X., Ostergaard, O., Ostrowski, M., Park, J., Thery, C., Pegtel, D. M., Witwer, K. W., Peinado, H., Aikawa, E., Perut, F., Alcaraz, M. J., Pfaffl, M. W., Anderson, J. D., Phinney, D. G., Andriantsitohaina, R., Pieters, B. C., Antoniou, A., Pink, R. C., Arab, T., Pisetsky, D. S., Archer, Fabienne, Strandmann, E., Atkin-Smith, G. K., Polakovicova, I., Ayre, D. C., Poon, I. K., Bach, J. M., Powell, B. H., Bachurski, D., Prada, I., Baharvand, H., Pulliam, L., Balaj, L., Quesenberry, P., Baldacchino, S., Radeghieri, A., Bauer, N. N., Raffai, R. L., Baxter, A. A., Raimondo, S., Bebawy, M., Rak, J., Beckham, C., Ramirez, M. I., Bedina Zavec, A., Raposo, G., Benmoussa, A., Rayyan, M. S., Berardi, A. C., Regev-Rudzki, N., Bergese, P., Ricklefs, F. L., Bielska, E., Robbins, P. D., Blenkiron, C., Roberts, D. D., Bobis-Wozowicz, S., Rodrigues, S. C., Boilard, E., Rohde, E., Boireau, W., Rome, S., Bongiovanni, A., Rouschop, K. M., Borras, F. E., Rughetti, A., Bosch, S., Russell, A. E., Boulanger, C. M., Saa, P., Breakefield, X., Sahoo, S., Breglio, A. M., Salas-Huenuleo, E., Brennan, M. A., Brigstock, D. R., Saugstad, J. A., Brisson, A., Saul, M. J., Broekman, M. L., Schiffelers, R. M., Bromberg, J. F., Schneider, R., Bryl-Gorecka, P., Schoyen, T. H., Buch, S., Scott, A., Buck, A. H., Shahaj, E., Burger, D., Sharma, S., Busatto, S., Shatnyeva, O., Buschmann, D., Shekari, F., Bussolati, B., Shelke, G. V., Buzas, E. I., Shetty, A. K., Byrd, J. B., Shiba, K., Camussi, G., Siljander, P. R., Carter, D. R., Silva, A. M., Caruso, S., Skowronek, A., Chamley, L. W., Snyder, O. L., Chang, Y. T., Soares, R. P., Chen, C., Sodar, B. W., Chen, S., Soekmadji, C., Cheng, L., Sotillo, J., Chin, A. R., Stahl, P. D., Clayton, A., Stoorvogel, W., Clerici, S. P., Stott, S. L., Cocks, A., Strasser, E. F., Cocucci, E., Swift, S., Coffey, R. J., Tahara, H., Cordeiro-Da-Silva, A., Tewari, M., Couch, Y., Timms, K., Coumans, F. A., Tiwari, S., Coyle, B., Tixeira, R., Crescitelli, R., Tkach, M., Criado, M. F., Toh, W. S., D'Souza-Schorey, C., Tomasini, R., Das, S., Torrecilhas, A. C., Datta Chaudhuri, A., Tosar, J. P., Candia, P., Toxavidis, V., De Santana, E. F., Urbanelli, L., De Wever, O., Vader, P., Del Portillo, H. A., Balkom, B. W., Demaret, T., Grein, S. G., Deville, S., Van Deun, J., Devitt, A., Herwijnen, M. J., Dhondt, B., Van Keuren-Jensen, K., Di Vizio, D., Niel, G., Dieterich, L. C., Royen, M. E., Dolo, V., Wijnen, A. J., Dominguez Rubio, A. P., Vasconcelos, M. H., Dominici, M., Vechetti, I. J., Dourado, M. R., Veit, T. D., Driedonks, T. A., Vella, L. J., Duarte, F. V., Velot, E., Duncan, H. M., Verweij, F. J., Eichenberger, R. M., Vestad, B., Ekstrom, K., Vinas, J. L., El Andaloussi, S., Visnovitz, T., Elie-Caille, C., Vukman, K. V., Erdbrugger, U., Wahlgren, J., Falcon-Perez, J. M., Watson, D. C., Fatima, F., Wauben, M. H., Fish, J. E., Weaver, A., Flores-Bellver, M., Webber, J. P., Forsonits, A., Weber, V., Frelet-Barrand, A., Wehman, A. M., Fricke, F., Weiss, D. J., Fuhrmann, G., Welsh, J. A., Gabrielsson, S., Wendt, S., Gamez-Valero, A., Wheelock, A. M., Gardiner, C., Wiener, Z., Gartner, K., Witte, L., Gaudin, R., Wolfram, J., Gho, Y. S., Xagorari, A., Giebel, B., Xander, P., Gilbert, C., Xu, J., Gimona, M., Yan, X., Giusti, I., Yanez-Mo, M., Goberdhan, D. C., Yin, H., Gorgens, A., Yuana, Y., Gorski, S. M., Zappulli, V., Greening, D. W., Zarubova, J., Gross, J. C., Zekas, V., Gualerzi, A., Zhang, J. Y., Gupta, G. N., Zhao, Z., Gustafson, D., Zheng, L., Handberg, A., Zheutlin, A. R., Haraszti, R. A., Zickler, A. M., Harrison, P., Zimmermann, P., Hegyesi, H., Zivkovic, A. M., Hendrix, A., Zocco, D., Hill, A. F., Zuba-Surma, E. K., Hochberg, F. H., Hoffmann, K. F., Holder, B., Holthofer, H., Hosseinkhani, B., Hu, G., Huang, Y., Huber, V., Hunt, S., Ibrahim, A. G., Ikezu, T., Inal, J. M., Isin, M., Ivanova, A., Jackson, H. K., Jacobsen, S., Jay, S. M., Jayachandran, M., Jenster, G., Jiang, L., Johnson, S. M., Jones, J. C., Jong, A., Jovanovic-Talisman, T., Jung, S., Kalluri, R., Kano, S. I., Kaur, S., Kawamura, Y., Keller, E. T., Khamari, D., Khomyakova, E., Khvorova, A., Kierulf, P., Kim, K. P., Kislinger, T., Klingeborn, M., Klinke, D. J., Kornek, M., Kosanovic, M. M., Kovacs, A. F., Kramer-Albers, E. M., Krasemann, S., Krause, M., Kurochkin, I. V., Kusuma, G. D., Kuypers, S., Laitinen, S., Langevin, S. M., Languino, L. R., Lannigan, J., Lasser, C., Laurent, L. C., Lavieu, G., Lazaro-Ibanez, E., Le Lay, S., Lee, M. S., Lee, Y. X. F., Lemos, D. S., Lenassi, M., Leszczynska, A., Li, I. T., Liao, K., Libregts, S. F., Ligeti, E., Lim, R., Lim, S. K., Line, A., Linnemannstons, K., Llorente, A., Lombard, C. A., Lorenowicz, M. J., Lorincz, A. M., Lotvall, J., Lovett, J., Lowry, M. C., Loyer, X., Lu, Q., Lukomska, B., Lunavat, T. R., Maas, S. L., Malhi, H., Marcilla, A., Mariani, J., Mariscal, J., Martens-Uzunova, E. S., Martin-Jaular, L., Martinez, M. C., Martins, V. R., Mathieu, M., Sanchez, Catherine

المساهمون: Infections Virales et Pathologie Comparée - UMR 754 (IVPC), Institut National de la Recherche Agronomique (INRA)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, INSERM, Institut Curie, INCa INCA-11548, French National Research Agency ANR-10-IDEX-0001-02 PSL*, ANR-11-LABX-0043, SIDACTION 17-1-AAE-1138, Fondation ARC PGA1 RF20180206962, PJA 20171206453, NIDA DA040385, DA047807, Ministry of Education, NIA AG057430, NIMH MH118164

المصدر: Journal of Extracellular Vesicles ; https://hal-univ-lyon1.archives-ouvertes.fr/hal-02191898Test ; Journal of Extracellular Vesicles, 2018, 7 (1), pp.1535750. ⟨10.1080/20013078.2018.1535750⟩

مصطلحات موضوعية: ectosomes, exosomes, extracellular vesicles, guidelines, microparticles, microvesicles, minimal information requirements, reproducibility, rigor, standardization, [SDV]Life Sciences [q-bio]

الوصف: International audience ; The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/30637094; hal-02191898; https://hal-univ-lyon1.archives-ouvertes.fr/hal-02191898Test; https://hal-univ-lyon1.archives-ouvertes.fr/hal-02191898/documentTest; https://hal-univ-lyon1.archives-ouvertes.fr/hal-02191898/file/2018_Thery_Journal%20of%20Extracellular%20Vesicles_1.pdfTest; PUBMEDCENTRAL: PMC6322352; PRODINRA: 472082; WOS: 000452068900001; PUBMED: 30637094

الإتاحة: https://doi.org/10.1080/20013078.2018.1535750Test
https://hal-univ-lyon1.archives-ouvertes.fr/hal-02191898Test
https://hal-univ-lyon1.archives-ouvertes.fr/hal-02191898/documentTest
https://hal-univ-lyon1.archives-ouvertes.fr/hal-02191898/file/2018_Thery_Journal%20of%20Extracellular%20Vesicles_1.pdfTest

المؤلفون: Ricklefs, F. L., Speranza, M. C., Hayes, J., Balaj, L., Breakefield, X. O., Bronisz, A., Carter, B., Freeman, G., Lawler, S., Chiocca, E. A.

المصدر: Neuro-Oncology ; volume 19, issue suppl_3, page iii50-iii50 ; ISSN 1522-8517 1523-5866

مصطلحات موضوعية: Cancer Research, Neurology (clinical), Oncology

الإتاحة: https://doi.org/10.1093/neuonc/nox036.181Test
http://academic.oup.com/neuro-oncology/article-pdf/19/suppl_3/iii50/17679073/nox036.181.pdfTest

المؤلفون: Kalra, H, Simpson, RJ, Ji, H, Aikawa, E, Altevogt, P, Askenase, P, Bond, VC, Borras, FE, Breakefield, X, Budnik, V, Buzas, E, Camussi, G, Clayton, A, Cocucci, E, Falcon-Perez, JM, Gabrielsson, S, Gho, YS, Gupta, D, Harsha, HC, Hendrix, A, Hill, AF, Inal, JM, Jenster, G, Kramer-Albers, EM, Lim, SK, Llorente, A, Lotvall, J, Marcilla, A, Mincheva-Nilsson, L, Nazarenko, I, Nieuwland, R, Hoen, ENM, Pandey, A, Patel, T, Piper, MG, Pluchino, S, Prasad, TSK, Rajendran, L, Raposo, G, Record, M, Reid, GE, Sanchez-Madrid, F, Schiffelers, RM, Siljander, P, Stensballe, A, Stoorvogel, W, Taylor, D, Thery, C, Valadi, H, van Balkom, BWM, Vazquez, J, Vidal, M, Wauben, MHM, Yanez-Mo, M, Zoeller, M, Mathivanan, S

المصدر: PLoS biology. 10(12):e1001450

مصطلحات موضوعية: Medicin och hälsovetenskap

الوصول الحر: http://kipublications.ki.se/Default.aspx?queryparsed=id:125920551Test