المؤلفون: Klein, Jean-Philippe, Mery, Lionel, Boudard, Delphine, Ravel, Célia, Cottier, Michèle, Bitounis, Dimitrios

المساهمون: Centre Hospitalier Universitaire de Saint-Etienne CHU Saint-Etienne (CHU ST-E), Santé Ingénierie Biologie Saint-Etienne (SAINBIOSE), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Universitaire de Rennes CHU Rennes = Rennes University Hospital Ponchaillou, Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique EHESP (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), École des Hautes Études en Santé Publique EHESP (EHESP)

المصدر: ISSN: 1661-6596.

مصطلحات موضوعية: nanosized objects, testicular biodistribution, reprotoxicity, blood-testis barrier, [SDV]Life Sciences [q-bio]

الوصف: International audience ; The real impact of nanoparticles on male fertility is evaluated after a careful analysis of the available literature. The first part reviews animal models to understand the testicular biodistribution and biopersistence of nanoparticles, while the second part evaluates their in vitro and in vivo biotoxicity. Our main findings suggest that nanoparticles are generally able to reach the testicle in small quantities where they persist for several months, regardless of the route of exposure. However, there is not enough evidence that they can cross the blood-testis barrier. Of note, the majority of nanoparticles have low direct toxicity to the testis, but there are indications that some might act as endocrine disruptors. Overall, the impact on spermatogenesis in adults is generally weak and reversible, but exceptions exist and merit increased attention. Finally, we comment on several methodological or analytical biases which have led some studies to exaggerate the reprotoxicity of nanoparticles. In the future, rigorous clinical studies in tandem with mechanistic studies are needed to elucidate the real risk posed by nanoparticles on male fertility.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/36614018; hal-03967605; https://hal.science/hal-03967605Test; https://hal.science/hal-03967605/documentTest; https://hal.science/hal-03967605/file/ijms-24-00576.pdfTest; PUBMED: 36614018

الإتاحة: https://doi.org/10.3390/ijms24010576Test
https://hal.science/hal-03967605Test
https://hal.science/hal-03967605/documentTest
https://hal.science/hal-03967605/file/ijms-24-00576.pdfTest

المؤلفون: Sancey, Lucie, Sabido, Odile, He, Zhiguo, Rossetti, Fabien, Guignandon, Alain, Bin, Valérie, Coll, Jean-Luc, Cottier, Michèle, Lux, François, Tillement, Olivier, Constant, Samuel, Mas, Christophe, Boudard, Delphine

المساهمون: Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire CHU Grenoble (CHUGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Santé Ingénierie Biologie Saint-Etienne (SAINBIOSE), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Biologie, Ingénierie et Imagerie pour l'Ophtalmologie (BIIO), Université Jean Monnet - Saint-Étienne (UJM), iLM - Formation, élaboration de nanomatériaux et cristaux (iLM - FENNEC), Institut Lumière Matière Villeurbanne (ILM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), NH TherAguix SA Meylan, Epithelix SARL Geneva, Switzerland, Université de Genève = University of Geneva (UNIGE), OncoTheis SARL Geneva, Switzerland, Service de Cytologie - Histologie Rénale - Embryologie CHU Saint-Etienne (UF 6725 - UF 6726), Centre Hospitalier Universitaire de Saint-Etienne CHU Saint-Etienne (CHU ST-E)-Université Jean Monnet - Saint-Étienne (UJM)

المصدر: ISSN: 1477-3155 ; Journal of Nanobiotechnology ; https://inserm.hal.science/inserm-02941434Test ; Journal of Nanobiotechnology, 2020, 18 (1), pp.129. ⟨10.1186/s12951-020-00683-6⟩.

مصطلحات موضوعية: AGuIX ® nanoparticles, 3D human healthy and tumor airway models, Nanoparticle's toxicity, Nanoparticle's uptake, Tumor targeting, [SDV]Life Sciences [q-bio], [SDV.BIO]Life Sciences [q-bio]/Biotechnology

الوصف: International audience ; Liquid deposit mimicking surface aerosolization in the airway is a promising strategy for targeting bronchopulmonary tumors with reduced doses of nanoparticle (NPs). In mimicking and studying such delivery approaches, the use of human in vitro 3D culture models can bridge the gap between 2D cell culture and small animal investigations. Here, we exposed airway epithelia to liquid-apical gadolinium-based AGuIX ® NPs in order to determine their safety profile. We used a multiparametric methodology to investigate the NP's distribution over time in both healthy and tumor-bearing 3D models. AGuIX ® NPs were able to target tumor cells in the absence of specific surface functionalization, without evidence of toxicity. Finally, we validated the therapeutic potential of this hybrid theranostic AGuIX ® NPs upon radiation exposure in this model. In conclusion, 3D cell cultures can efficiently mimic the normal and tumor-bearing airway epitheliums, providing an ethical and accessible model for the investigation of nebulized NPs.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/32912214; inserm-02941434; https://inserm.hal.science/inserm-02941434Test; https://inserm.hal.science/inserm-02941434/documentTest; https://inserm.hal.science/inserm-02941434/file/80-2020-Mucil_OncoCilAir.pdfTest; PUBMED: 32912214

الإتاحة: https://doi.org/10.1186/s12951-020-00683-6Test
https://inserm.hal.science/inserm-02941434Test
https://inserm.hal.science/inserm-02941434/documentTest
https://inserm.hal.science/inserm-02941434/file/80-2020-Mucil_OncoCilAir.pdfTest

المؤلفون: Sabido, Odile, Figarol, Agathe, Klein, Jean-Philippe, Bin, Valérie, Forest, Valérie, Pourchez, Jérémie, Fubini, Bice, Cottier, Michèle, Tomatis, Maura, Boudard, Delphine

المساهمون: Santé Ingénierie Biologie Saint-Etienne (SAINBIOSE), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Lyon, Centre commun de Cytométrie en Flux (CCCF), Université Jean Monnet - Saint-Étienne (UJM), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté COMUE (UBFC)-Université Bourgogne Franche-Comté COMUE (UBFC), Università degli studi di Torino = University of Turin (UNITO)

المصدر: ISSN: 2079-4991.

مصطلحات موضوعية: +33-477421441 (O.S.), carbon nanotubes, acid functionalization, annealing treatment, oxidative stress, mitochondrial membrane potential collapse, cytotoxicity, chromatin decondensation, scavenging capacity, flow cytometry and spin trapping in-cell free system, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Conventional nanotoxicological assays are subjected to various interferences with nanoparticles and especially carbon nanotubes. A multiparametric flow cytometry (FCM) methodology was developed here as an alternative to quantify oxidative stress, mitochondrial impairment, and later cytotoxic and genotoxic events. The experiments were conducted on RAW264.7 macrophages, exposed for 90 min or 24 h-exposure with three types of multiwalled carbon nanotubes (MWCNTs): pristine (Nanocyl™ CNT), acid functionalized (CNTf), or annealed treatment (CNTa). An original combination of reactive oxygen species (ROS) probes allowed the simultaneous quantifications of broad-spectrum ROS, superoxide anion (O2•−), and hydroxyl radical (•OH). All MWCNTs types induced a slight increase of broad ROS levels regardless of earlier antioxidant catalase activity. CNTf strongly stimulated the O2•− production. The •OH production was downregulated for all MWCNTs due to their scavenging capacity. The latter was quantified in a cell-free system by electron paramagnetic resonance spectroscopy (EPR). Further FCM-based assessment revealed early biological damages with a mitochondrial membrane potential collapse, followed by late cytotoxicity with chromatin decondensation. The combined evaluation by FCM analysis and cell-free techniques led to a better understanding of the impacts of MWCNTs surface treatments on the oxidative stress and related biological response.

العلاقة: hal-03740357; https://hal.science/hal-03740357Test; https://hal.science/hal-03740357/documentTest; https://hal.science/hal-03740357/file/Sabido%20et%20al,%202020,%20Nanomaterials.pdfTest

الإتاحة: https://doi.org/10.3390/nano10020319Test
https://hal.science/hal-03740357Test
https://hal.science/hal-03740357/documentTest
https://hal.science/hal-03740357/file/Sabido%20et%20al,%202020,%20Nanomaterials.pdfTest

المؤلفون: Boudard, Delphine, Aureli, Federica, Laurent, Blandine, Sturm, Nathalie, Raggi, Andrea, Antier, Emilie, Lakhdar, Latifa, Marche, Patrice N., Cottier, Michèle, Cubadda, Francesco, Bencsik, Anna

المصدر: Kidney International Reports ; volume 5, issue 4, page 554-558 ; ISSN 2468-0249

مصطلحات موضوعية: Nephrology

الإتاحة: https://doi.org/10.1016/j.ekir.2019.12.005Test
https://api.elsevier.com/content/article/PII:S2468024919315906?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2468024919315906?httpAccept=text/plainTest

المؤلفون: Mercier, Clément, Jacqueroux, Elodie, He, Zhiguo, Hodin, Sophie, Constant, Samuel, Perek, Nathalie, Boudard, Delphine, Delavenne, Xavier

المساهمون: Biologie Intégrative du Tissu Osseux (LBTO), Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'Études de l'Europe Médiane (CEEM EA 2521), Institut National des Langues et Civilisations Orientales (Inalco)

المصدر: ISSN: 0939-6411 ; European Journal of Pharmaceutics and Biopharmaceutics ; https://hal.science/hal-03484728Test ; European Journal of Pharmaceutics and Biopharmaceutics, 2019, 139, pp.186 - 196. ⟨10.1016/j.ejpb.2019.04.002⟩.

مصطلحات موضوعية: [SDV]Life Sciences [q-bio]

الوصف: International audience

العلاقة: hal-03484728; https://hal.science/hal-03484728Test; https://hal.science/hal-03484728/documentTest; https://hal.science/hal-03484728/file/S0939641118314681.pdfTest; PII: S0939-6411(18)31468-1

الإتاحة: https://doi.org/10.1016/j.ejpb.2019.04.002Test
https://hal.science/hal-03484728Test
https://hal.science/hal-03484728/documentTest
https://hal.science/hal-03484728/file/S0939641118314681.pdfTest

المؤلفون: Boudard, Delphine, Aureli, Federica, Laurent, Blandine, Sturm, Nathalie, Raggi, Andrea, Antier, Emilie, Lakhdar, Latifa, Marche, Patrice, N., Cottier, Michèle, Cubadda, Francesco, Bencsik, Anna

المساهمون: Service de Cytologie - Histologie Rénale - Embryologie CHU Saint-Etienne (UF 6725 - UF 6726), Centre Hospitalier Universitaire de Saint-Etienne CHU Saint-Etienne (CHU ST-E)-Université Jean Monnet - Saint-Étienne (UJM), Biologie Intégrative du Tissu Osseux (LBTO), Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Istituto Superiore di Sanità = National Institute of Health (ISS), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire CHU Grenoble (CHUGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 ), Plateforme d’expérimentation animale (PFEA), Laboratoire de Lyon ANSES, Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université de Lyon, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)

المصدر: ISSN: 2468-0249 ; Kidney International Reports ; https://inserm.hal.science/inserm-02334051Test ; Kidney International Reports, 2019, 4 (10), pp.1463-1471. ⟨10.1016/j.ekir.2019.06.007⟩.

مصطلحات موضوعية: chronic oral exposure, E551, kidney, mice, nanoparticles, silica, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, [SDV.MHEP.UN]Life Sciences [q-bio]/Human health and pathology/Urology and Nephrology

الوصف: International audience ; Introduction: Silicon dioxide, produced as synthetic amorphous silica (SAS), is made of nanoparticles (NPs), either present as such or as agglomerates and aggregates, and is widely used in many types of food processes and products as an additive. To assess whether repeated, long-term exposure to SAS NPs may result in adverse effects, mice were exposed for 18 months via drinking water to NM-200, one of the reference nanostructured silica used for applications related to food, at 4.8 mg NM-200/kg body weight per day, a dose relevant to the estimated dietary exposure to SAS in humans.Methods: The experiment focused on the kidney and liver as target organs and was carried out in parallel using 3 mouse lines (wild type and transgenic) differing for the expression of a-synuclein, that is, murine and human mutated (A53T). Sensitive determination of silicon revealed higher contents in liver and kidneys of NM-200–exposed mice compared with unexposed aged-matched controls.Results: Histological abnormalities, such as vacuolization of tubular epithelial cells, were detected in all kidneys, as well as inflammatory responses that were also detected in livers of exposed animals. Less frequent but more deleterious, amyloidosis lesions were observed in glomeruli, associated with perivascular amyloid accumulation in liver.Conclusion: These histological findings, in conjunction with the observation of detectable deposition of silica, highlight that chronic oral intake of SAS may pose a health risk to humans and need to be examined further.

العلاقة: inserm-02334051; https://inserm.hal.science/inserm-02334051Test; https://inserm.hal.science/inserm-02334051/documentTest; https://inserm.hal.science/inserm-02334051/file/Boudard_KIReports_2019_in%20press.pdfTest

الإتاحة: https://doi.org/10.1016/j.ekir.2019.06.007Test
https://inserm.hal.science/inserm-02334051Test
https://inserm.hal.science/inserm-02334051/documentTest
https://inserm.hal.science/inserm-02334051/file/Boudard_KIReports_2019_in%20press.pdfTest

المؤلفون: Klein, Jean-Philippe, Mery, Lionel, Boudard, Delphine, Ravel, Célia, Cottier, Michèle, Bitounis, Dimitrios

المساهمون: Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E), Santé Ingénierie Biologie Saint-Etienne (SAINBIOSE), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Pontchaillou [Rennes], Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), École des Hautes Études en Santé Publique [EHESP] (EHESP)

المصدر: International Journal of Molecular Sciences
International Journal of Molecular Sciences, 2023, 24 (1), ⟨10.3390/ijms24010576⟩

مصطلحات موضوعية: nanosized objects, blood-testis barrier, [SDV]Life Sciences [q-bio], reprotoxicity, testicular biodistribution

الوصف: International audience; The real impact of nanoparticles on male fertility is evaluated after a careful analysis of the available literature. The first part reviews animal models to understand the testicular biodistribution and biopersistence of nanoparticles, while the second part evaluates their in vitro and in vivo biotoxicity. Our main findings suggest that nanoparticles are generally able to reach the testicle in small quantities where they persist for several months, regardless of the route of exposure. However, there is not enough evidence that they can cross the blood-testis barrier. Of note, the majority of nanoparticles have low direct toxicity to the testis, but there are indications that some might act as endocrine disruptors. Overall, the impact on spermatogenesis in adults is generally weak and reversible, but exceptions exist and merit increased attention. Finally, we comment on several methodological or analytical biases which have led some studies to exaggerate the reprotoxicity of nanoparticles. In the future, rigorous clinical studies in tandem with mechanistic studies are needed to elucidate the real risk posed by nanoparticles on male fertility.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::2126cbe8eb209c88b880441c52965856Test
https://pubmed.ncbi.nlm.nih.gov/36614018Test

المؤلفون: Bitounis, Dimitrios, Klein, Jean-Philippe, Mery, Lionel, Maurice Edouard, El Merhie, Amira, Forest, Valérie, Boudard, Delphine, Pourchez, Jérémie, Cottier, Michèle

المساهمون: Santé Ingénierie Biologie Saint-Etienne (SAINBIOSE), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Biologie de la Reproduction CHU Saint Etienne, Centre Hospitalier Universitaire de Saint-Etienne CHU Saint-Etienne (CHU ST-E)-Université Jean Monnet - Saint-Étienne (UJM), Université de Lyon, F-69000 Lyon, France, Service de Cytologie - Histologie Rénale - Embryologie CHU Saint-Etienne (UF 6725 - UF 6726)

المصدر: ISSN: 0003-2654.

مصطلحات موضوعية: [SDV]Life Sciences [q-bio], [SDV.IB]Life Sciences [q-bio]/Bioengineering

الوصف: International audience ; Increasing consumption of engineered nanoparticles and occupational exposure to novel, ultrafine airborne particles during the last decades has coincided with deterioration of sperm parameters and delayed fecundity. In order to prevent possible adverse health effects and ensure a sustainable growth for the nanoparticle industry, the ability to investigate the nanosized, mineralogical load of human reproductive systems is becoming a real clinical need. Toward this goal, the current study proposes two methods for the detection and quantification of engineered nanoparticles in human follicular and seminal fluid, developed with the use of well-defined 60 nm Au particles. Despite the complexity of these biological fluids, simple physical and chemical treatments allow for the precise quantification of more than 50 and 70% wt of the spiked Au nanoparticles at low μg ml−1 levels in follicular and seminal fluids, respectively. The use of electron microscopy for the detailed observation of the detected analytes is also enabled. The proposed method is applied on a small patient cohort in order to demonstrate its clinical applicability by exploring the differences in the metal and particulate content between patients with normal and low sperm count.

العلاقة: hal-01896549; https://hal.science/hal-01896549Test; https://hal.science/hal-01896549/documentTest; https://hal.science/hal-01896549/file/HAL_Bitounis%20et%20al_2018_Analyst.pdfTest

الإتاحة: https://doi.org/10.1039/c7an01641gTest
https://hal.science/hal-01896549Test
https://hal.science/hal-01896549/documentTest
https://hal.science/hal-01896549/file/HAL_Bitounis%20et%20al_2018_Analyst.pdfTest

المؤلفون: Bitounis, Dimitrios, Barnier, Vincent, Guibert, Cyril, Pourchez, Jérémie, Forest, Valérie, Boudard, Delphine, Hochepied, Jean-François, Chelle, Pierre, Vergnon, Jean-Michel, Cottier, Michèle

المساهمون: Santé Ingénierie Biologie Saint-Etienne (SAINBIOSE), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Georges Friedel (LGF-ENSMSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Service de Pneumologie et Oncologie thoracique CHU Saint-Etienne, Centre Hospitalier Universitaire de Saint-Etienne CHU Saint-Etienne (CHU ST-E)-Université Jean Monnet - Saint-Étienne (UJM), Université de Lyon, F-69000 Lyon, France, Service de Cytologie - Histologie Rénale - Embryologie CHU Saint-Etienne (UF 6725 - UF 6726), Centre des Matériaux (CDM), Mines Paris - PSL (École nationale supérieure des mines de Paris), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 2040-3364.

مصطلحات موضوعية: [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], [SDV]Life Sciences [q-bio], [SDV.IB]Life Sciences [q-bio]/Bioengineering

الوصف: International audience ; Bronchoalveolar lavage (BAL) is a diagnostic procedure which samples the cellular and non-cellular components of the pulmonary epithelial surface. The inherent biological noise of BAL fluids inhibits their direct mineralogical analysis while currently available particle retrieval protocols are suspected to impose quantitative and qualitative bias on the studied particle load. This study presents a simple method for the near-lossless extraction of citrate-capped gold nanoparticles from human BAL fluids at sub-ppm levels which enables their quantitation and surface characterization. This procedure was modeled according to fundamental principles of particle sedimentation and liquid–liquid interdiffusion and was evaluated by a battery of analytical techniques. The extraction yield of gold nanoparticles ranged from 61 to 86%, with a quantitation limit at 0.5 μg ml−1, as measured by inductively-coupled optical emission spectroscopy. Dynamic light scattering could resolve the hydrodynamic size distribution of extracted particles which returned significantly different photon count rates at various concentrations. Their shape and primary size were easily observable by electron microscopy while atomic force microscopy, Auger electron spectroscopy and X-ray photoelectron spectroscopy could respectively probe the particles’ biomolecular corona, detect surface-adsorbed S- and N- species, and identify carbon-based covalent bonds.

العلاقة: hal-01806118; https://minesparis-psl.hal.science/hal-01806118Test; https://minesparis-psl.hal.science/hal-01806118/documentTest; https://minesparis-psl.hal.science/hal-01806118/file/HAL_Bitounis%20et%20al_2018_Nanoscale.pdfTest

الإتاحة: https://doi.org/10.1039/C7NR04484DTest
https://minesparis-psl.hal.science/hal-01806118Test
https://minesparis-psl.hal.science/hal-01806118/documentTest
https://minesparis-psl.hal.science/hal-01806118/file/HAL_Bitounis%20et%20al_2018_Nanoscale.pdfTest

المؤلفون: Kurtz-Chalot, Andréa, Villiers, Christian, L., Pourchez, Jérémie, Boudard, Delphine, Martini, Matteo, Marche, Patrice, Cottier, Michèle, Forest, Valérie

المساهمون: Santé Ingénierie Biologie Saint-Etienne (SAINBIOSE), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire CHU Grenoble (CHUGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 ), Institut Lumière Matière Villeurbanne (ILM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 0928-4931.

مصطلحات موضوعية: [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering

الوصف: International audience ; Nanoparticles (NP) physico-chemical features greatly influence NP/cell interactions. NP surface functionalization is often used to improve NP biocompatibility or to enhance cellular uptake. But in biological media, the formation of a protein corona adds a level of complexity. The aim of this study was to investigate in vitro the influence of NP surface functionalization on their cellular uptake and the biological response induced. 50nm fluorescent silica NP were functionalized either with amine or carboxylic groups, in presence or in absence of polyethylene glycol (PEG). NP were incubated with macrophages, cellular uptake and cellular response were assessed in terms of cytotoxicity, pro-inflammatory response and oxidative stress. The NP protein corona was also characterized by protein mass spectroscopy. Results showed that NP uptake was enhanced in absence of PEG, while NP adsorption at the cell membrane was fostered by an initial positively charged NP surface. NP toxicity was not correlated with NP uptake. NP surface functionalization also influenced the formation of the protein corona as the profile of protein binding differed among the NP types.

العلاقة: hal-01680498; https://hal.science/hal-01680498Test; https://hal.science/hal-01680498/documentTest; https://hal.science/hal-01680498/file/2017_HAL_Kurtz-Chalot_MSEC.pdfTest

الإتاحة: https://doi.org/10.1016/j.msec.2017.02.028Test
https://hal.science/hal-01680498Test
https://hal.science/hal-01680498/documentTest
https://hal.science/hal-01680498/file/2017_HAL_Kurtz-Chalot_MSEC.pdfTest